Betel nut biotin extraction process

Abstract

The present application relates to the field of biotin extraction, and particularly relates to a betel nut biotin extraction process, comprising: detecting the dehydration stability and the dehydration reference value to determine the biotin dehydration state; determining to detect and analyze the defective dehydrated substance or to adjust the filtering parameter according to the biotin dehydration state; detecting the proportion of the number of each defect point and the maximum penetration depth to determine the defect point state of the defective dehydrated substance; determining to adjust the belt rotating speed or to adjust the heating parameter according to the defect point state; adjusting the filtering parameter according to the dehydration stability of the defective dehydrated substance; and effectively selecting the dehydration parameter of the betel nut biotin according to the actual dehydration effect, so as to avoid the problems that the solid betel nut biotin is prone to have uneven internal and external humidity or the internal molecular activity of the betel nut biotin is lost due to the setting deviation of the dehydration parameter, and the extraction effect of the betel nut biotin is improved.

Description

Technical Field

[0001] This invention relates to the field of biotin extraction, and more particularly to a biotin extraction process from areca nut. Background Technology

[0002] Areca biotin refers to the bioactive components found in areca nuts, mainly including arecoline, flavonoids, tannins, triterpenoids, polysaccharides, and polyphenols. These components possess various biological activities, such as antioxidant, antibacterial, anti-inflammatory, and antidepressant effects. Among them, arecoline is the main active ingredient in areca nuts, with anthelmintic and antidepressant effects. Areca flavonoids and polysaccharides are also important bioactive components, possessing antioxidant and immunomodulatory effects. In addition, areca nuts also contain various vitamins and minerals, such as vitamin C, vitamin B6, calcium, and iron, which are also beneficial to human health. Currently, the areca nut food processing industry is gradually adopting areca biotin as an additive in some foods. However, in the actual extraction process of areca biotin, the processing and extraction parameters cannot be adjusted according to the actual extraction effect. Therefore, how to effectively extract areca biotin is a problem that urgently needs to be solved by those skilled in the art.

[0003] Chinese Patent Publication No. CN111793098A discloses a method for extracting areca biotin from young areca nuts, comprising: (1) taking young areca nuts, drying them at low temperature, and pulverizing them; (2) extracting them sequentially with 35-40%, 55-60%, and 80-85% ethanol solutions at 15-30 MPa for 8-12 hours, combining the filtrates; adding 2-3 times the amount of water and a compound bio-enzyme, adjusting the pH, enzymatically extracting, filtering, and obtaining a crude extract; (3) in a water bath at 35-40℃, at 60-90 r / Under magnetic stirring at a speed of min, adjust the pH and keep stirring for 10-20 min, centrifuge and take the supernatant extract; (4) concentrate and dry at low temperature under reduced pressure to the water content; It can be seen that the method of extracting areca biotin from areca nut fruit has the following problems: low temperature reduced pressure concentration and drying are slow for dehydrating areca biotin, and the obtained solid areca biotin is prone to uneven internal and external humidity. However, the drying parameters with higher temperatures in the prior art are prone to over-drying and causing loss of molecular activity in areca biotin. Summary of the Invention

[0004] Therefore, the present invention provides a biotin extraction process for areca nut to overcome the problem in the prior art that the dehydration parameters of areca nut biotin cannot be effectively selected according to the actual dehydration effect, which leads to uneven internal and external humidity or loss of molecular activity in solid areca nut biotin.

[0005] To achieve the above objectives, the present invention provides a biotin extraction process for areca nut, comprising:

[0006] Dehydration stability and dehydration reference values ​​were measured to determine the dehydration status of biotin.

[0007] Based on the biotin dehydration status, determine the detection and analysis of defective dehydrated products, or adjust the filtration parameters;

[0008] The defect status of the dehydrated material is determined by detecting the percentage of each defect location and the maximum puncture depth.

[0009] Depending on the condition of the defect location, adjustments can be made to either the belt speed or the heating parameters.

[0010] The filtration parameters are adjusted based on the dehydration stability of the defective dehydrated material.

[0011] The amount of belt speed adjustment is related to the proportion of defect locations;

[0012] Heating parameters include peak heating temperature and heating rate;

[0013] The filtration parameters include the settling filtration time and the centrifugal filtration time.

[0014] Furthermore, the analytical processing method is determined based on the biotin dehydration status, including detecting and analyzing defective dehydrated products, or adjusting filtration parameters.

[0015] Furthermore, the dehydration state of biotin is determined based on the dehydration stability and the dehydration reference value, including the dehydration reference value being greater than the preset dehydration reference value and the dehydration stability being greater than the preset dehydration stability, or the dehydration reference value being less than or equal to the preset dehydration reference value.

[0016] Furthermore, in the detection and analysis of defective dehydrated products, the adjustment method of heating parameters is determined according to the defect location status of the defective dehydrated products, including adjusting the belt speed or adjusting the heating parameters.

[0017] Furthermore, the defect status of the dehydrated material is determined based on the proportion of each defect status and the maximum puncture depth. The defect status includes either a proportion of defect status greater than or equal to a preset proportion of defect status and a maximum puncture depth less than a preset maximum puncture depth, or a maximum puncture depth greater than or equal to a preset maximum puncture depth.

[0018] Furthermore, in adjusting the belt speed, the belt speed is reduced based on the proportion of defect locations.

[0019] The decrease in belt speed is positively correlated with the proportion of defect locations.

[0020] Furthermore, in adjusting the heating parameters, the peak heating temperature is increased based on the maximum puncture depth, and the heating rate is decreased based on the proportion of defect locations.

[0021] The increase in the peak heating temperature is positively correlated with the maximum penetration depth;

[0022] The decrease in heating rate is positively correlated with the proportion of defect locations.

[0023] Furthermore, in adjusting the dehydration time, the filtration parameters are adjusted according to the dehydration stability of the defective dehydrated material, including adjusting the settling filtration time or the centrifugal filtration time.

[0024] Furthermore, in adjusting the settling and filtration time, the settling and filtration time is increased based on the dehydration stability.

[0025] The increase in the static filtration time is negatively correlated with the dehydration stability.

[0026] The dehydration stability is within a first preset dehydration stability range.

[0027] Furthermore, in adjusting the centrifugal filtration time, the centrifugal filtration time is increased based on the dehydration stability.

[0028] The increase in centrifugal filtration time is negatively correlated with the dehydration stability.

[0029] The dehydration stability is within a second preset dehydration stability range.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows: In the technical solution of the present invention, the dehydration state of biotin is determined according to the dehydration stability and the dehydration reference value. The dehydration state of biotin reflects whether the dehydration effect of solid areca biotin meets the standard. Among them, the dehydration stability reflects the difference in dehydration effect between different batches of solid areca biotin, and the dehydration reference value reflects the water content of a single batch of solid areca biotin. Different analysis and processing methods are selected according to different biotin dehydration states, making the analysis and processing methods more in line with the actual working scenario, further improving the selection accuracy of areca biotin dehydration parameters, and thus improving the extraction efficiency of areca biotin.

[0031] Furthermore, in this invention, the state of defect points in the dehydrated material is determined based on the proportion of each defect point and the maximum puncture depth. The maximum puncture depth reflects the difference in the uniformity of dehydration between the inside and outside of the solid areca biotin, and the heating parameters are adjusted accordingly to avoid the problem that the surface of the solid areca biotin is too dehydrated due to the high initial heating temperature, resulting in poor internal dehydration effect, thereby improving the uniformity of dehydration of areca biotin.

[0032] Furthermore, in this invention, the overall dehydration effect of areca biotin is reflected by the proportion of defect locations. Compared with dehydration detection by a single test, this improves the effectiveness of data judgment. By reducing the belt speed, the dehydration effect of areca biotin is improved.

[0033] Furthermore, in this invention, the filtration parameters are adjusted based on the dehydration stability of the defective dehydrated material. The dehydration stability of the defective dehydrated material reflects the degree of filter cloth blockage in the belt dehydrator, and thus reflects the filtration effect of the areca biotin solution. Different filtration parameters are selected and adjusted according to different degrees of filtration effect, so that the filtration parameters are more in line with the actual working scenario. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the areca biotin extraction process according to an embodiment of the present invention;

[0035] Figure 2 This is a flowchart illustrating how the analytical processing method is determined based on the biotin dehydration state, as described in an embodiment of the present invention.

[0036] Figure 3 This is a flowchart illustrating how filtration parameters are adjusted based on the dehydration stability of the defective dehydrated material according to an embodiment of the present invention. Detailed Implementation

[0037] To make the objectives and advantages of the present invention clearer, the present invention will be further described below with reference to embodiments; it should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention.

[0038] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0039] It should be noted that in the description of this invention, the terms "upper", "lower", "left", "right", "inner", "outer", etc., which indicate directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. This is only for the convenience of description and is not intended to indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention.

[0040] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0041] Please see Figures 1 to 3 As shown, the present invention provides a biotin extraction process for areca nut, comprising:

[0042] Dehydration stability and dehydration reference values ​​were measured to determine the dehydration status of biotin.

[0043] Based on the biotin dehydration status, determine the detection and analysis of defective dehydrated products, or adjust the filtration parameters;

[0044] The defect status of the dehydrated material is determined by detecting the percentage of each defect location and the maximum puncture depth.

[0045] Depending on the condition of the defect location, adjustments can be made to either the belt speed or the heating parameters.

[0046] The filtration parameters are adjusted based on the dehydration stability of the defective dehydrated material.

[0047] The amount of belt speed adjustment is related to the proportion of defect locations;

[0048] Heating parameters include peak heating temperature and heating rate;

[0049] The filtration parameters include the settling filtration time and the centrifugal filtration time.

[0050] The extraction of areca biotin in this invention includes:

[0051] Wash the fresh areca nuts, crush them, and pass them through a 25-mesh sieve to obtain fresh areca nut powder;

[0052] The areca nut powder was successively extracted with ethanol solutions of 30%, 50% and 80% by mass concentration, and cold-soaked for 12 hours at 30 MPa pressure. The filtrates were then combined and the ratio of areca nut powder to ethanol solution was 1:20 g / mL.

[0053] Add 3 times the amount of water to the filtrate, add 0.5% of compound biological enzyme by weight, adjust the pH to 6.0 with 5% hydrochloric acid solution, and carry out enzymatic extraction at 40℃ for 3 hours. Filter to obtain crude extract, and let the crude extract settle.

[0054] The crude extract that had been allowed to stand for 1 hour was centrifuged. The crude extract was then stirred for 20 minutes in a 40°C water bath with magnetic stirring at 10000 r / min. The supernatant was collected and recorded as areca biotin solution.

[0055] A belt vacuum dehydrator was used to dry the areca biotin solution to obtain a solid areca biotin extract. The solid areca biotin extract was then pulverized to obtain the areca biotin to be prepared.

[0056] Specifically, the analytical processing method is determined based on the biotin dehydration status, including detecting and analyzing defective dehydrated products, or adjusting filtration parameters.

[0057] Specifically, the biotin dehydration state is determined based on the dehydration stability and the dehydration reference value, including a first preset biotin dehydration state in which the dehydration reference value is greater than the preset dehydration reference value and the dehydration stability is greater than the preset dehydration stability, or a second preset biotin dehydration state in which the dehydration reference value is less than or equal to the preset dehydration reference value.

[0058] If the dehydration reference value is greater than the preset dehydration reference value and the dehydration stability is greater than the preset dehydration stability, the filtration parameters are adjusted. If the dehydration reference value is less than or equal to the preset dehydration reference value, the defective dehydrated material is detected and analyzed.

[0059] If the dehydration reference value is greater than the preset dehydration reference value and the dehydration stability is less than or equal to the preset dehydration stability, then the biotin dehydration status is qualified.

[0060] Specifically, a monitoring cycle is set for the extraction process, and the dehydration status of biotin is tested at the end of each monitoring cycle. A near-infrared spectrometer is installed above the conveyor section of the belt vacuum dehydrator. Near-infrared spectroscopy analysis technology is used to achieve non-contact detection of the water content of areca biotin extract on the conveyor belt. This includes the near-infrared spectrometer collecting the spectrum of solid areca biotin extract on the conveyor belt, comparing the collected spectral data with a standard spectral database, and determining the water content of areca biotin extract through spectral matching and algorithm calculation. The unit is %, and the dehydration reference value is 1 / water content. The preset dehydration reference value is 1 / (5%). The dehydration stability is the water collection volume corresponding to the current monitoring cycle - the water collection volume corresponding to the most recent monitoring cycle. The preset dehydration stability is 10% of the water collection volume corresponding to the most recent monitoring cycle. The water collection volume is the water collected by the water collection device of the belt vacuum dehydrator.

[0061] Specifically, in the detection and analysis of defective dehydrated products, the adjustment method of heating parameters is determined according to the defect location status of the defective dehydrated products, including adjusting the belt speed or adjusting the heating parameters.

[0062] Specifically, the defect status of the dehydrated material is determined based on the proportion of each defect location and the maximum puncture depth. The defect status includes either a proportion of defect locations greater than or equal to a preset proportion of defect locations and a maximum puncture depth less than a preset maximum puncture depth, or a maximum puncture depth greater than or equal to a preset maximum puncture depth.

[0063] If the proportion of defective points is greater than or equal to the preset proportion of defective points and the maximum puncture depth is less than the preset maximum puncture depth, the belt speed will be adjusted; if the maximum puncture depth is greater than or equal to the preset maximum puncture depth, the heating parameters will be adjusted.

[0064] If the percentage of defective points is greater than or equal to the preset percentage of defective points and the maximum puncture depth is greater than or equal to the preset maximum puncture depth, then the belt dewatering machine will be tested for faults.

[0065] Specifically, at the end of each monitoring cycle, a preset weight of areca biotin sample is extracted from the conveyor section of the vacuum dehydrator. A texture analyzer is used to randomly select several detection points on the areca biotin sample at a preset pressure, and the puncture depth is recorded. For a single detection point, if the corresponding puncture depth is greater than the preset puncture depth, then that detection point is a defect point. The maximum puncture depth is the maximum value of the puncture depths corresponding to several detection points for a single areca biotin sample. The preset puncture depth is 30% of the thickness of the areca biotin sample, and the preset maximum puncture depth is 50% of the thickness of the areca biotin sample. The preset pressure value allows the user to use different pressures for texture analyzer testing of solid areca biotin extract that meets the user's dehydration requirements. The maximum pressure of the texture analyzer corresponding to a puncture depth less than 30% of the thickness of the areca biotin sample is recorded as the preset pressure. The percentage of defect points = number of defect points / number of detection points.

[0066] Specifically, in adjusting the belt speed, the belt speed is reduced according to the proportion of defect points.

[0067] The decrease in belt speed is positively correlated with the proportion of defect locations.

[0068] Specifically, the belt rotation speed is the speed at which the conveyor belt of the belt vacuum dewatering machine moves.

[0069] Specifically, in adjusting the heating parameters, the peak heating temperature is increased based on the maximum puncture depth, and the heating rate is decreased based on the proportion of defect locations.

[0070] The increase in the peak heating temperature is positively correlated with the maximum penetration depth;

[0071] The decrease in heating rate is positively correlated with the proportion of defect locations.

[0072] Specifically, the processing environment of the belt vacuum dehydrator is equipped with a temperature control device to control the processing environment temperature. The temperature of the temperature control device changes cyclically. Specifically, the temperature control device is set with a minimum heating temperature and a peak heating temperature. In a single heating process, the heating temperature of the temperature control device gradually increases from the minimum heating temperature to the peak heating temperature, and then gradually decreases from the peak heating temperature to the minimum heating temperature. This heating process is repeated, and the heating rate is the time required for a single heating process.

[0073] Specifically, in adjusting the dehydration time, the filtration parameters are adjusted according to the dehydration stability of the defective dehydrated material, including adjusting the settling filtration time or the centrifugal filtration time.

[0074] Specifically, in adjusting the settling and filtration time, the settling and filtration time is increased based on the dehydration stability.

[0075] The increase in the static filtration time is negatively correlated with the dehydration stability.

[0076] The dehydration stability is within a first preset dehydration stability range.

[0077] Specifically, in adjusting the centrifugal filtration time, the centrifugal filtration time is increased based on the dehydration stability.

[0078] The increase in centrifugal filtration time is negatively correlated with the dehydration stability.

[0079] The dehydration stability is within a second preset dehydration stability range.

[0080] Specifically, the preset dehydration stability range includes a first preset dehydration stability range and a second preset dehydration stability range. The values ​​in the first preset dehydration stability range are all greater than (1.5 × preset dehydration stability) and less than or equal to (2 × preset dehydration stability). The values ​​in the second preset dehydration stability range are all greater than (2 × preset dehydration stability).

[0081] The settling and filtration time is the same as the settling time of the crude extract, and the centrifugal filtration time is the same as the centrifugal separation time of the crude extract.

[0082] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of the present invention.

[0083] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A biotin extraction process from areca nut, characterized in that, include: Dehydration stability and dehydration reference values ​​were measured to determine the dehydration status of biotin. Dehydration reference value = 1 / moisture content, preset dehydration reference value is 1 / (5%), dehydration stability = water collection volume corresponding to the current monitoring cycle - water collection volume corresponding to the most recent monitoring cycle; Based on the biotin dehydration status, determine the detection and analysis of defective dehydrated products, or adjust the filtration parameters; If the dehydration reference value is greater than the preset dehydration reference value and the dehydration stability is greater than the preset dehydration stability, the filtration parameters are adjusted. If the dehydration reference value is less than or equal to the preset dehydration reference value, the defective dehydrated material is detected and analyzed. If the dehydration reference value is greater than the preset dehydration reference value and the dehydration stability is less than or equal to the preset dehydration stability, then the biotin dehydration status is qualified. In the detection and analysis of defective dehydrated products, the adjustment method of heating parameters is determined according to the defect location status of the defective dehydrated products, including adjusting the belt speed or adjusting the heating parameters. The defect status of the dehydrated material is determined by detecting the percentage of each defect location and the maximum puncture depth. Depending on the condition of the defect location, adjustments can be made to either the belt speed or the heating parameters. The filtration parameters are adjusted based on the dehydration stability of the defective dehydrated material. Heating parameters include peak heating temperature and heating rate; Filtration parameters include settling time and centrifugal filtration time; In adjusting the belt speed, the belt speed is reduced based on the proportion of defective points. The decrease in belt speed is positively correlated with the proportion of defect locations. In adjusting the heating parameters, the peak heating temperature is increased based on the maximum puncture depth, and the heating rate is decreased based on the proportion of defect locations. The increase in the peak heating temperature is positively correlated with the maximum penetration depth; The decrease in heating rate is positively correlated with the proportion of defect locations. In adjusting the dehydration time, the filtration parameters are adjusted according to the dehydration stability of the defective dehydrated product, including adjusting the settling filtration time or the centrifugal filtration time. In the adjustment of the settling and filtration time, if the dehydration stability is within the first preset dehydration stability range, the settling and filtration time is increased according to the dehydration stability. The increase in the static filtration time is negatively correlated with the dehydration stability. In the adjustment of the centrifugal filtration time, if the dehydration stability is within the second preset dehydration stability range, the centrifugal filtration time is increased according to the dehydration stability. The increase in centrifugal filtration time is negatively correlated with the stability of the dehydrated product. Among them, the values ​​within the first preset dehydration stability range are all greater than 1.5 × preset dehydration stability and less than or equal to 2 × preset dehydration stability, and the values ​​within the second preset dehydration stability range are all greater than 2 × preset dehydration stability.

2. The areca biotin extraction process according to claim 1, characterized in that, The analytical treatment method is determined based on the biotin dehydration status, including detecting and analyzing defective dehydrated products, or adjusting filtration parameters.

3. The areca biotin extraction process according to claim 2, characterized in that, The dehydration state of biotin is determined based on dehydration stability and dehydration reference value, including dehydration reference value greater than preset dehydration reference value and dehydration stability greater than preset dehydration stability, or dehydration reference value less than or equal to preset dehydration reference value.

4. The areca biotin extraction process according to claim 3, characterized in that, The status of defect points in the dehydrated material is determined based on the proportion of each defect point and the maximum puncture depth. The status of defect points includes either a proportion of defect points greater than or equal to a preset proportion of defect points and a maximum puncture depth less than a preset maximum puncture depth, or a maximum puncture depth greater than or equal to a preset maximum puncture depth.

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