Microfluidic device and microfluidic detection device

Abstract

The present invention belongs to the field of microfluidic control technology, in particular to a microfluidic device and a microfluidic detection device. The microfluidic device includes a main body and a flow channel switching assembly; the main body is provided with a plurality of flow channels; the flow channel switching assembly includes an upper sealing plate and a lower sealing plate, the upper sealing plate and the lower sealing plate are respectively attached to the upper and lower sides of the main body, the upper sealing plate is provided with a plurality of upper liquid troughs, and the lower sealing plate is provided with a plurality of lower liquid troughs; the flow channel switching assembly is rotatably connected to the main body, and the flow channel switching assembly is configured to be rotatable relative to the main body to adjust the position of the upper liquid trough and the lower liquid trough so that at least two of the multiple flow channels are connected via the upper liquid trough and / or the lower liquid trough. Among them, the flow channel switching assembly adopts a double-layer sealing plate structure to realize the switching flow channel connection, occupies less space, has a more compact structure, is conducive to miniaturized design, and is easy to operate.

Description

Technical Field

[0001] The present invention belongs to the field of microfluid control technology, in particular to a microfluidic device and a microfluidic detection device. Background Art

[0002] Microfluidics, the technology for controlling, manipulating, and detecting complex fluids at microscopic scales, is a new interdisciplinary field developed from the foundations of microelectronics, micromechanics, bioengineering, and nanotechnology. Experiments in biology, chemistry, materials science, and other fields frequently require manipulation of fluids. For example, DNA sample preparation, liquid chromatography, PCR reactions, and electrophoresis detection are all performed in liquid environments. If sample preparation, biochemical reactions, and result detection are integrated onto a biochip, the volume of fluid used in the experiment must be reduced from milliliters to microliters, making powerful microfluidic devices essential. Microfluidics devices offer a wide range of applications in biotechnology research due to their compact size, low sample / reagent requirements, fast reaction speed, massively parallel processing, and disposable design.

[0003] However, existing microfluidic devices generally use a single flow channel structure, which can be controlled by valves. To switch multiple flow channels on and off, existing microfluidic devices use multiple valve control structures, which are complex, costly, and cumbersome to operate. Summary of the Invention

[0004] The present invention provides a microfluidic device and a microfluidic detection device to solve the technical problems in the prior art such as the overly complex multi-channel control structure.

[0005] A first aspect of the present invention provides a microfluidic device, which includes a main body and a flow channel switching assembly; the main body is provided with multiple flow channels; the flow channel switching assembly includes an upper sealing plate and a lower sealing plate, the upper sealing plate and the lower sealing plate are respectively attached to the upper and lower sides of the main body, the upper sealing plate is provided with multiple upper liquid grooves, and the lower sealing plate is provided with multiple lower liquid grooves; the flow channel switching assembly is rotatably connected to the main body, and the flow channel switching assembly is configured to be rotatable relative to the main body to adjust the positions of the upper liquid grooves and the lower liquid grooves so that at least two of the multiple flow channels are connected via the upper liquid grooves and / or the lower liquid grooves.

[0006] In an optional solution of the present invention, the multiple flow channels include a first flow channel and a second flow channel, the second flow channel includes a second flow channel main road, and the first flow channel and the second flow channel main road are configured to be able to communicate through one of the multiple lower liquid tanks.

[0007] In an optional solution of the present invention, the second flow channel also includes a first branch of the second flow channel and a second branch of the second flow channel, and the first branch of the second flow channel and the second branch of the second flow channel intersect at the second flow channel main channel; the multiple flow channels also include a third flow channel, and the third flow channel is configured to be able to communicate with the first branch of the second flow channel or the second branch of the second flow channel through one of the multiple upper liquid tanks.

[0008] In an optional scheme of the present invention, the multiple upper liquid troughs include a first liquid trough and a second liquid trough; the multiple lower liquid troughs include a third liquid trough, a fourth liquid trough and a fifth liquid trough; the flow channel switching component is configured to rotate to a first position, and the fourth liquid trough connects the first flow channel and the second flow channel main road; the flow channel switching component is configured to rotate to a second position, and the third liquid trough connects the first flow channel and the second flow channel main road, and the first liquid trough connects the second branch of the second flow channel and the third flow channel; the flow channel switching component is configured to rotate to a fourth position, and the third liquid trough connects the first flow channel and the second flow channel, and the second liquid trough connects the first branch of the second flow channel and the third flow channel.

[0009] In an optional solution of the present invention, the multiple flow channels further include a fourth flow channel; and when the flow channel switching assembly is configured to rotate to a third position, the second liquid transfer trough connects the third flow channel and the fourth flow channel.

[0010] In an optional solution of the present invention, the main body is provided with a limiting structure; the flow channel switching assembly includes a limiting component, and during the rotation of the flow channel switching assembly relative to the main body, the stop position of the limiting component is determined by the limiting structure, so that the flow channel switching assembly switches between the first position, the second position, the third position and the fourth position.

[0011] In an optional solution of the present invention, the limiting mechanism includes a first limiting groove, a second limiting groove and a slide groove; when the limiting component is configured to switch to the first limiting groove, the flow channel switching assembly is located in the first position; when the limiting component is configured to switch to the second limiting groove, the flow channel switching assembly is located in the second position; the limiting component is configured to slide along the slide groove to enable the flow channel switching assembly to switch between the third position and the fourth position.

[0012] In an optional solution of the present invention, the chute includes a first chute section and a second chute section; the limiting component is configured to slide along the first chute section to rotate the flow channel switching assembly to a third position; the limiting component is configured to slide along the second chute section to rotate the flow channel switching assembly to a fourth position.

[0013] In an optional solution of the present invention, the groove depth of the sliding groove is greater than the groove depths of the first limiting groove and the second limiting groove.

[0014] In an optional solution of the present invention, the flow channel switching assembly also includes a rotating body and a rotating shaft. The rotating body is rotatably connected to the main body through the rotating shaft. The limiting component and the upper sealing plate are connected to the rotating body and can rotate with the rotating body; the two ends of the rotating shaft are respectively connected to the rotating body and the lower sealing plate, and the lower sealing plate can rotate with the rotating shaft.

[0015] In an optional solution of the present invention, the rotating body is provided with a shaft insertion hole, which is a waist-shaped hole. The radial cross-section of the top end of the shaft is waist-shaped and can be inserted into the shaft insertion hole.

[0016] In an optional solution of the present invention, a tray is provided at the bottom end of the rotating shaft, and the lower sealing plate is connected to the tray.

[0017] In an optional solution of the present invention, the projection of the upper sealing plate in the up-down direction at least covers the lower sealing plate.

[0018] In an optional solution of the present invention, the first flow channel includes a second flow channel opening arranged on the lower surface of the main body; the second flow channel main line includes a third flow channel opening arranged on the lower surface of the main body; wherein the second flow channel opening and the third flow channel opening are overlapped with the lower sealing plate in the upper and lower directions.

[0019] In an optional scheme of the present invention, the first branch of the second flow channel includes a fourth flow channel opening arranged on the upper surface of the main body; the second branch of the second flow channel includes a fifth flow channel opening arranged on the upper surface of the main body; the third flow channel includes a sixth flow channel opening and a seventh flow channel opening arranged on the upper surface of the main body; the fourth flow channel includes a ninth flow channel opening arranged on the upper surface of the main body; the fourth flow channel opening, the fifth flow channel opening, the sixth flow channel opening, the seventh flow channel opening and the ninth flow channel opening are overlapped with the upper sealing plate in the upper and lower directions.

[0020] In an optional solution of the present invention, the first flow channel includes a first flow channel opening, which is located on one side of the flow channel switching component; the microfluidic device also includes a fluid storage component, which is connected to the body and communicates with the first flow channel opening.

[0021] In an optional solution of the present invention, the third flow channel includes an eighth flow channel opening, which is located on one side of the flow channel switching assembly and is used to discharge the fluid in the third flow channel.

[0022] In an optional solution of the present invention, the third flow channel is a flow channel that extends in a circuitous manner and is used to collect fluid.

[0023] In an optional solution of the present invention, the fourth flow channel includes a tenth flow channel opening, and the tenth flow channel opening is located on one side of the flow channel switching assembly.

[0024] In an optional solution of the present invention, the main body includes an upper plate, a flow channel plate and a lower plate, and the upper and lower surfaces of the flow channel plate are provided with flow channel grooves; the upper plate and the lower plate are respectively arranged on the upper and lower sides of the flow channel plate to form multiple flow channels together with the flow channel grooves.

[0025] The second aspect of the present invention provides a microfluidic detection device, which includes a sensing device and the above-mentioned microfluidic device; the sensing device is arranged at the second flow channel main trunk and connected to the main body, and the sensing device and the main body form the corresponding part of the second flow channel main trunk to form a sensing area.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The microfluidic device provided by the present invention includes a main body and a flow channel switching component. Multiple flow channels are provided in the main body, and the flow channel switching component is used to switch different flow channels on and off. In particular, the flow channel switching component adopts a double-layer sealing plate structure to achieve the switching flow channel connection, occupies less space, has a more compact structure, is conducive to miniaturized design, and is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1a A schematic diagram of a microfluidic detection device according to one embodiment of the present disclosure;

[0030] Figure 1b for Figure 1a A cross-sectional view of the microfluidic detection device in FIG.

[0031] Figure 1c for Figure 1a One of the exploded views of the microfluidic detection device in [1].

[0032] Figure 1d for Figure 1a The second exploded view of the microfluidic detection device;

[0033] Figure 2a for Figure 1a Schematic diagram of the microfluidic device in;

[0034] Figure 2b for Figure 2a One of the exploded views of the microfluidic device in [1].

[0035] Figure 2c for Figure 2a Exploded view of the microfluidic device (part 2);

[0036] Figure 2d for Figure 2c The third exploded view of the microfluidic device;

[0037] Figure 2e for Figure 2c A local enlarged view of Q in FIG;

[0038] Figure 2f for Figure 2d The local enlarged view of X in the figure;

[0039] Figure 3a for Figure 2a Schematic diagram of the flow channel switching component in;

[0040] Figure 3b for Figure 3a Exploded view of the flow channel switching component in;

[0041] Figure 4a A partial exploded view of a microfluidic device according to one embodiment of the present disclosure;

[0042] Figure 4b for Figure 4a Another exploded view of the microfluidic device in FIG;

[0043] Figure 4c for Figure 4b An exploded view of the microfluidic device from another perspective;

[0044] Figure 5a A simplified diagram of the flow path layout inside a body is provided according to one embodiment of the present invention;

[0045] Figure 5b A simplified diagram of the communication relationship between different flow channels when the flow channel switching assembly is rotated to the first position;

[0046] Figure 5c A simplified diagram of the communication relationship between different flow channels when the flow channel switching assembly is rotated to the second position;

[0047] Figure 5d A simplified diagram of the communication relationship between different flow channels when the flow channel switching assembly is rotated to the third position;

[0048] Figure 5e A simplified diagram of the communication relationship between different flow channels when the flow channel switching assembly is rotated to the fourth position;

[0049] Figure 6 (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid tank, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid tank when the limiting component is located in the first limiting groove;

[0050] Figure 7 (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid tank, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid tank when the limiting component is located in the second limiting groove;

[0051] Figure 8 (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid trough, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid trough when the limiting component is located at the end of the chute close to the second limiting groove;

[0052] Figure 9 (a) and (b) respectively show the relative position relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid trough, and the relative position relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid trough when the limiting component is located at one end of the slide groove close to the first limiting groove.

[0053] Reference numerals

[0054] 1000. Microfluidic detection device;

[0055] 100, microfluidic device; 110, body; 111, upper plate; 112, flow channel plate; 113, lower plate; 1131, first sealing gasket guide post; 1132, second sealing gasket guide post; 120, flow channel switching assembly; 121, rotating body; 122, rotating shaft; 1221, tray; 123, sealing plate; 124, limiting component; 125, lower sealing plate; 130, fluid storage component; 140, membrane; 150, conductive sheet; 160, sealing patch;

[0056] 200, sensing device; 210, carrier board; 220, sensing chip; 230, probe;

[0057] 300, sealing gasket;

[0058] P1, first flow channel; P2, second flow channel; P21, first branch of second flow channel; P22, second branch of second flow channel; P23, main trunk of second flow channel; P231, first section of second flow channel; P232, second section of second flow channel; P233, third section of second flow channel; P3, third flow channel; P4, fourth flow channel;

[0059] C1, first flow channel outlet; C2, second flow channel outlet; C3, third flow channel outlet; C4, fourth flow channel outlet; C5, fifth flow channel outlet; C6, sixth flow channel outlet; C7, seventh flow channel outlet; C8, eighth flow channel outlet; C9, ninth flow channel outlet; C10, tenth flow channel outlet; C11, eleventh flow channel outlet; C12, twelfth flow channel outlet;

[0060] B1, first liquid trough; B2, second liquid trough; B3, third liquid trough; B4, fourth liquid trough; B5, fifth liquid trough;

[0061] V1, first limiting groove; V2, second limiting groove; V3, sliding groove;

[0062] G1, first runner; G11, first section of first runner; G12, second section of first runner; G2, second runner; G21, first section of second runner; G211, first buffer; G22, second section of second runner; G221, second buffer; G23, third section of second runner; G24, fourth section of second runner; G25, fifth section of second runner; G3, third runner; G31, first section of third runner; G32, second section of third runner; G33, third section of third runner; G4, fourth runner;

[0063] H1, first through hole; H2, second through hole; H3, third through hole; H4, fourth through hole; H5, fifth through hole; H6, sixth through hole;

[0064] D1, the first liquid outlet; D2, the second liquid outlet;

[0065] L, liquid storage tank; R, sealed chamber; K, shaft plug hole;

[0066] A1, sensing area; A2, flow channel buffer area; A3, observation area. DETAILED DESCRIPTION

[0067] In order to make the above and other features and advantages of the present invention more clear, the present invention is further described below with reference to the accompanying drawings. It should be understood that the specific embodiments given herein are for the purpose of explaining to those skilled in the art and are only exemplary and not restrictive.

[0068] Figure 1a Schematic diagram of a microfluidic detection device 1000 according to one embodiment of the present disclosure. Figure 1a The microfluidic detection device 1000 includes a microfluidic device 100 and a sensing device 200 . The sensing device 200 is connected to the microfluidic device 100 and is located below the microfluidic device 100 .

[0069] Figure 2a for Figure 1a Schematic diagram of the microfluidic device 100. Figure 2a The microfluidic device 100 includes a body 110 and a flow channel switching assembly 120. The body 110 is provided with multiple flow channels. Due to the complex flow channel layout, in order to facilitate understanding of the disclosed solution, Figure 5a A simplified diagram of the flow path layout inside the body 110 is provided as an example.

[0070] See also Figure 5a The main body 110 is provided with a first flow channel P1, a second flow channel P2, a third flow channel P3, a fourth flow channel P4 and a sealed chamber R. Each flow channel is independent of each other and includes a plurality of flow channel openings.

[0071] In the present disclosure, the first flow channel P1 includes a first flow channel opening C1 and a second flow channel opening C2, the second flow channel P2 includes a third flow channel opening C3, a fourth flow channel opening C4 and a fifth flow channel opening C5, the third flow channel P3 includes a sixth flow channel opening C6, a seventh flow channel opening C7 and an eighth flow channel opening C8, and the fourth flow channel includes a ninth flow channel opening C9 and a tenth flow channel opening C10.

[0072] The second flow channel P2 includes a first branch P21, a second branch P22, and a main trunk P23. The first branch P21 and the second branch P22 converge at the main trunk P23. As can be seen in the present disclosure, the second flow channel P2 is a bifurcated flow channel having two branches. The main trunk P23 includes a first section P231 and a second section P232. The first section P231 includes a first liquid port D1, and the second section P232 includes a second liquid port D2. The first liquid port D1 and the second liquid port D2 are spaced apart and located at the ends of the first section P231 and the second section P232, respectively.

[0073] Figure 1b for Figure 1a A cross-sectional view of the microfluidic detection device 1000 in FIG. Figure 1c for Figure 1a One of the exploded views of the microfluidic detection device 1000, Figure 1d for Figure 1a The second exploded view of the microfluidic detection device 1000 in FIG. Figure 1b to Figure 1d In the present disclosure, the sensing device 200 is connected to the body 110. The first and second liquid ports D1 and D2 are located on the lower surface of the body 110. The vertical projection of the sensing device 200 covers the first and second liquid ports D1 and D2, forming the third section P233 of the second flow channel. The third section P233 connects the first section P231 and the second section P232 of the second flow channel, forming a complete second flow channel trunk P23. It can be understood that fluid passing through the third section P233 of the second flow channel can be detected by the sensing device 200 and generate a signal. Therefore, a sensing area A1 is formed in the third section P233 of the second flow channel.

[0074] See also Figure 1aThe main body 110 is provided with a transparent observation area A3, which is located above the sensing area A1. In a specific application, the projection of the observation area A3 in the upper and lower directions covers the first liquid port D1 and the second liquid port D2, so as to facilitate the observation of the fluid conditions at the sensing area A1.

[0075] See also Figure 1b to Figure 1d In order to prevent fluid leakage in the third section P233 of the second flow channel, in the present disclosure, the microfluidic detection device 1000 also includes a sealing gasket 300, which is located between the main body 110 and the sensing device 200. The sealing gasket 300 is an annular structure and is located on the outside of the liquid outlet, and is arranged around the sensing area A1.

[0076] In the present disclosure, a sealing gasket guide column extends downward from the main body 110, and the sealing gasket guide column includes a first sealing gasket guide column 1131 and a second sealing gasket guide column 1132 arranged in parallel and spaced apart. The sealing gasket 300 is correspondingly provided with a guide column plug-in hole. The installation position of the sealing gasket 300 is determined by cooperating with the guide column plug-in hole to achieve quick installation.

[0077] It should be noted that the sealed chamber R can pre-seal the fluid. In the present disclosure, the sealed chamber R is located below the first branch P21 of the second flow channel. The fluid sealed in the sealed chamber R and the fluid flowing through the first branch P21 of the second flow channel can perform ion transfer, thereby forming a detectable signal.

[0078] In actual applications, a membrane 140 is provided between the sealed chamber R and the first branch of the second flow channel P21, separating the sealed chamber R from the first branch of the second flow channel P21. The membrane 140 is configured to allow ions to pass through, thereby allowing only ions to pass between the fluid in the sealed chamber R and the fluid in the first branch of the second flow channel P21. The membrane 140 can be a semipermeable membrane or a highly permeable membrane, such as an ion exchange membrane or a proton exchange membrane, as long as it allows ions to pass through. There is no specific limitation on this.

[0079] See also Figure 5a Furthermore, the first branch P21 of the second flow channel is provided with a flow buffer zone A2, which is first gradually expanded and then gradually contracted along the extension direction of the first branch P21 of the second flow channel. It can be understood that the groove width at the flow buffer zone A2 is wider, and accordingly, the fluid flow rate at the flow buffer zone A2 is reduced. Preferably, the sealed chamber R is located below the flow buffer zone A2, and the membrane 140 is located between the sealed chamber R and the flow buffer zone A2. In this way, the fluid in the first branch P21 of the second flow channel is decelerated in the flow buffer zone A2, avoiding the excessive flow rate affecting the ion exchange with the fluid in the sealed chamber R, thereby ensuring signal stability. It can be seen that the cross-sectional shape of the flow channel can be continuous and consistent, or it can be variable, and there is no specific limitation.

[0080] See also Figure 1d In the present disclosure, the body 110 further includes a conductive sheet 150 , which is located below the membrane 140 . The signal generated by ion exchange in the sealed chamber R can be transmitted to the outside through the conductive sheet 150 .

[0081] See also Figure 1c In the present disclosure, sensing device 200 includes a carrier 210, a sensing chip 220, and a probe 230. As previously mentioned, sealing gasket 300 is an annular structure disposed around sensing area A1, thereby forming a sealing gasket opening. Sensing area A1 is located within the sealing gasket opening, and the upper surface of sensing chip 220 can be embedded within the sealing gasket opening. Therefore, sensing chip 220 is located within sensing area A1 and can be wetted by the fluid in the third section P233 of the second flow channel.

[0082] The probe 230 is connected to the carrier 210 and its top end is connected to the conductive sheet 150. The signal transmitted by the conductive sheet 150 is transmitted to the carrier 210 through the probe 230. Secondly, the signal generated by the sensing chip 220 is also transmitted to the carrier 210, and then transmitted outward from the carrier 210 to the signal acquisition and analysis instrument, thereby completing the detection and analysis.

[0083] It should be noted that the conductive sheet 150 can be in various forms, such as a copper alloy sheet or an aluminum alloy sheet. The probe 230 can also be in various forms, as long as it can form an electrical connection with the conductive sheet 150. The carrier 210 can be a PCB to ensure signal transmission between the sensing chip 220 and the probe 230. The sensing chip 220 can be a biochip, which is integrated onto the carrier 210 to form the main body of the sensing device 200.

[0084] See also Figure 5a In this disclosure, each flow channel opening is located at the end of the corresponding flow channel. Specifically, the third flow channel opening C3 is located at the end of the second flow channel trunk P23, the fourth flow channel opening C4 is located at the end of the second flow channel first branch P21, and the fifth flow channel opening C5 is located at the end of the second flow channel second branch P22. It should be noted that the flow channel opening can also be located in the middle of the corresponding flow channel, and the specific location of the flow channel opening is not limited.

[0085] In the present disclosure, the flow channel switching assembly 120 is rotatably connected to the body 110, for example, via a shaft or gears. The flow channel switching assembly 120 is used to switch the communication between different flow channels. Specifically, the flow channel switching assembly 120 is provided with a liquid flow channel. The flow channel switching assembly 120 is configured to rotate relative to the body 110 to adjust the position of the liquid flow channel so that at least two of the multiple flow channels communicate via the liquid flow channel. It is understood that during the rotation of the flow channel switching assembly 120 relative to the body 110, the liquid flow channel rotates with the flow channel switching assembly 120, thereby adjusting its position. Figures 5b to 5e This is a simplified diagram of the connection relationship between different flow channels when the flow channel switching component 120 rotates to different special positions. Figures 5b to 5e The flow channel switching assembly 120 is provided with a first liquid transfer tank B1, a second liquid transfer tank B2, a third liquid transfer tank B3, a fourth liquid transfer tank B4 and a fifth liquid transfer tank B5.

[0086] See also Figure 5b When the flow channel switching assembly 120 rotates to the first position, the fourth liquid channel B4 connects the second flow channel opening C2 and the third flow channel opening C3, thereby connecting the first flow channel P1 and the second flow channel P2, and the third flow channel P3 and the fourth flow channel P4 are not connected to other flow channels, and the third flow channel P3 and the fourth flow channel P4 are independent.

[0087] In the first position, the fluid is injected through the first channel opening C1, passes through the first channel P1, the fourth liquid tank B4, the second channel first section P231, the sensing area A1 and the second channel second section P232, and then is divided into the second channel first branch P21 and the second channel second branch P22.

[0088] See also Figure 5c When the flow channel switching assembly 120 rotates to the second position, the first liquid channel B1 connects the fifth flow channel opening C5 and the sixth flow channel opening C6, and the third liquid channel B3 connects the second flow channel opening C2 and the third flow channel opening C3, thereby connecting the first flow channel P1, the second flow channel P2 and the third flow channel P3 in sequence, and the fourth flow channel P4 is not connected to other flow channels, and the fourth flow channel P4 is independent.

[0089] As can be seen, in the second position, the second flow channel P2 is connected to the third flow channel P3 via the second flow channel second branch P22. After being injected through the first flow channel opening C1, the fluid passes through the first flow channel P1, the third liquid transfer tank B3, the second flow channel first section P231, the sensing area A1, the second flow channel second section P232, the second flow channel second branch P22, and the first liquid transfer tank B1 before flowing into the third flow channel P3. This prevents the fluid from passing through the second flow channel first branch P21.

[0090] See also Figure 5d When the flow channel switching assembly 120 rotates to the third position, the second liquid channel B2 connects the seventh flow channel opening C7 and the ninth flow channel opening C9, thereby connecting the third flow channel P3 and the fourth flow channel P4. The first flow channel P1 and the second flow channel P2 are not connected to other flow channels, and the first flow channel P1 and the second flow channel P2 are independent.

[0091] See also Figure 5eWhen the flow channel switching assembly 120 rotates to the fourth position, the second liquid channel B2 connects the fourth flow channel opening C4 and the sixth flow channel opening C6, and the fifth liquid channel B5 connects the second flow channel opening C2 and the third flow channel opening C3, thereby connecting the first flow channel P1, the second flow channel P2 and the third flow channel P3 in sequence, and the fourth flow channel P4 is not connected to other flow channels, and the fourth flow channel P4 is independent.

[0092] As can be seen, in the fourth position, the second flow channel P2 is connected to the third flow channel P3 via the second flow channel first branch P21. After being injected through the first flow channel opening C1, the fluid passes through the first flow channel P1, the fifth liquid transfer tank B5, the second flow channel first section P231, the sensing area A1, the second flow channel second section P232, the second flow channel first branch P21, and the second liquid transfer tank B2 before flowing into the third flow channel P3. This prevents the fluid from passing through the second flow channel second branch P22.

[0093] As can be seen from the above, when the flow channel switching assembly 120 is in the first position, the fluid flows through the second flow channel main path P23 into the second flow channel first branch P21 and the second flow channel second branch P22. When the flow channel switching assembly 120 is in the second position, the fluid flows through the second flow channel main path P23 to the second flow channel second branch P22. When the flow channel switching assembly 120 is in the fourth position, the fluid flows through the second flow channel main path P23 to the second flow channel first branch P21. Thus, by rotating the flow channel switching assembly 120 to different specific positions, the flow direction of the fluid entering the second flow channel P2 can be changed.

[0094] It can be seen that when the injected fluid does not need to be connected with the pre-sealed fluid in the sealed chamber R, that is, the injected fluid is not to pass through the flow channel buffer zone A2, the flow channel switching component 120 is rotated to the second position, and the fluid introduced into the flow channel passes through the sensing area A1 and flows to the second branch P22 of the second flow channel. When the injected fluid needs to form a signal with the pre-sealed fluid in the sealed chamber R, the flow channel switching component 120 is rotated to the fourth position, and the fluid introduced into the flow channel passes through the sensing area A1 and flows to the first branch P21 of the second flow channel, thereby passing through the flow channel buffer zone A2 and performing ion exchange with the fluid in the sealed chamber R, thereby forming a detectable signal. It can be seen that in the present disclosure, by setting the second flow channel P2 as a forked flow channel structure, and cooperating with the flow channel switching component 120 to make the fluid in the second flow channel P2 flow to different branches, so as to adapt to the requirements of different biochemical reaction stages, the versatility is better and the application scenarios are wider.

[0095] In the present disclosure, the third flow channel P3 is a fluid collection area for collecting fluids. Its structure can vary. For example, the third flow channel P3 can include a reservoir for storing fluids, or it can be provided with a water-absorbing medium, such as absorbent cotton. In a preferred embodiment, the third flow channel P3 is a channel extending in a circuitous manner. In the illustrated embodiment, the third flow channel P3 has a serpentine structure, but this is not limiting. For example, a spiral structure is also possible.

[0096] It should be noted that in the present disclosure, the microfluidic detection device 1000 is provided with a first mode and a second mode. By rotating the flow channel switching component 120 to connect different flow channels, the microfluidic detection device 1000 switches between the first mode and the second mode. When the flow channel switching component 120 is in the first position or the second position, the microfluidic detection device 1000 is in the first mode. When the flow channel switching component 120 is in the third position or the fourth position, the microfluidic detection device 1000 is in the second mode. In actual applications, depending on the application scenario, the first mode is the administrator mode and the second mode is the user mode.

[0097] The first mode is used by manufacturers to perform pre-processing operations. Specifically, when the flow channel switching assembly 120 is in the first position, it is used to pre-encapsulate the fluid in the microcavity structure on the sensing chip 220. When the flow channel switching assembly 120 is in the second position, the injected fluid does not contact the membrane 140. For example, in the second position, oil can be injected without infiltrating the membrane 140, thus not affecting the permeability of the membrane 140.

[0098] In the second mode, the user performs injection or extraction operations. When the flow channel switching assembly 120 is in the fourth position, the user injects fluid, and the fluid injected by the user must pass through the membrane 140. When the flow channel switching assembly 120 is in the third position, the user extracts fluid.

[0099] As can be seen from the above, when the flow channel switching assembly 120 is in the second or fourth position, fluid passing through the sensing area A1 flows into the third flow channel P3 for temporary storage. It is understandable that the fluid in the third flow channel P3 can be extracted through the eighth flow channel opening C8. However, this operation will cause air to be drawn into the sensing area A1 from the first flow channel opening C1, thereby forming bubbles in the sensing area A1 and affecting the detection results. In actual applications, after the flow channel switching assembly 120 is switched to the third position, the fluid is extracted through the eighth flow channel opening C8 or the tenth flow channel opening C10. This isolates the second flow channel P2 and prevents the introduction of air into the sensing area A1.

[0100] See also Figures 5b to 5eThe second through seventh and ninth flow channel openings C2, C7, and C9 are transition flow channel openings, designed to interface with different flow channels. To ensure interoperability between the flow channel and the transition flow channel openings, the flow channel switching assembly 120's vertical projection covers the second through seventh flow channel openings C2, C7, and C9. In this disclosure, the flow channel is a long, narrow groove, but this is not limiting. The only requirement is that the flow channel and the transition flow channel openings are compatible and leak-proof.

[0101] In the present disclosure, the first flow channel opening C1, the eighth flow channel opening C8, and the tenth flow channel opening C10 are used for liquid injection or extraction. The first flow channel opening C1, the eighth flow channel opening C8, and the tenth flow channel opening C10 can be located on the same side or different sides of the flow channel switching assembly 120, as long as they are not within the vertical projection coverage area of ​​the flow channel switching assembly 120. It should be noted that the shape of the flow channel opening is not limited and can be adjusted according to actual needs. For example, it can be a round opening, a square opening, or a bell-shaped opening.

[0102] Figure 2b for Figure 2a One of the exploded views of the microfluidic device 100 in FIG. Figure 2b In the present disclosure, the microfluidic device 100 further includes a fluid storage component 130, which is connected to the main body 110 and communicates with the first flow channel opening C1. The fluid storage component 130 is provided with a liquid storage chamber for temporarily storing the fluid to be injected into the main body 110. In an optional embodiment, the liquid storage chamber in the fluid storage component 130 is funnel-shaped, wide at the top and narrow at the bottom, so that the fluid in the liquid storage chamber is concentrated toward the first flow channel opening C1. The fluid storage component 130 can be fixedly connected to the main body 110. Preferably, the fluid storage component 130 is detachably connected to the main body 110, so that it is easy to remove when the fluid storage component 130 is no longer needed.

[0103] As can be seen from the above, the flow channel switching assembly 120 can adopt a single-layer plate structure to achieve switching and connecting of the various flow channels. In this way, all the liquid flow channels are concentrated on the single-layer sealing plate. Accordingly, the surface area of ​​the sealing plate must be large enough to accommodate the arrangement of the liquid flow channels, thereby increasing the surface area of ​​the sealing plate covering the body 110 and correspondingly occupying more space. In order to make the flow channel switching assembly 120 more compact and occupy less space, in the present disclosure, the flow channel switching assembly 120 adopts a double-layer sealing plate structure.

[0104] Figure 2c for Figure 2a The second exploded view of the microfluidic device 100, Figure 2d for Figure 2c The third exploded view of the microfluidic device 100 is shown in FIG. Figure 2c and Figure 2dThe flow channel switching assembly 120 includes an upper sealing plate 123 and a lower sealing plate 125. The first liquid trough B1 and the second liquid trough B2 are upper liquid troughs and are arranged on the upper sealing plate 123. The third liquid trough B3, the fourth liquid trough B4 and the fifth liquid trough B5 are lower liquid troughs and are arranged on the lower sealing plate 125.

[0105] Figure 2e for Figure 2c A local enlarged view of Q in the figure, Figure 2f for Figure 2d See the enlarged view of the X in the figure. Figure 2e The fourth flow channel opening C4 to the seventh flow channel opening C7 and the ninth flow channel opening C9 are disposed on the upper surface of the body 110; please refer to Figure 2f The second flow channel opening C2 and the third flow channel opening C3 are disposed on the lower surface of the main body 110 .

[0106] As can be seen from the above, the upper liquid channel cooperates with the channel opening located on the upper surface of the body 110 to connect the third channel P3 with any of the second channel first branch P21, the second channel second branch P22, and the fourth channel P4. The lower liquid channel cooperates with the channel opening located on the lower surface of the body 110 to connect the first channel P1 with the second channel P2. Thus, by rotating the channel switching assembly 120 between the first and fourth positions, the positions of the upper and lower liquid channels are adjusted, so that at least two of the multiple channels are connected via the upper and / or lower liquid channels.

[0107] It can be seen that in the present disclosure, the flow channel switching component 120 adopts a double-layer sealing plate structure to achieve the switching flow channel connection. Compared with only using a single-layer sealing plate structure, the double-layer stacking layout structure occupies less space and has a more compact structure, which is conducive to miniaturized design and convenient operation.

[0108] Understandably, to prevent fluid leakage, the upper sealing plate 123 and the lower sealing plate 125 are respectively attached to the upper and lower sides of the body 110. Furthermore, the fourth through seventh flow openings C4, C7, and C9 are vertically overlapped with the upper sealing plate 123, while the second and third flow openings C2, C3 are vertically overlapped with the lower sealing plate 125. This ensures that the upper and lower liquid troughs can be rotated to their corresponding flow openings.

[0109] In the present disclosure, the main body 110 is provided with a limiting structure, and the flow channel switching assembly 120 includes a limiting component 124. During the rotation of the flow channel switching assembly 120 relative to the main body 110, the limiting component 124 also rotates together. The stop position of the limiting component 124 is determined by the limiting structure, so that the flow channel switching assembly 120 can switch between the first position, the second position, the third position and the fourth position.

[0110] See also Figures 2c to 2fThe limiting structure includes a limiting groove and a slide groove V3. The limiting component 124 can be a pin or a pre-loaded spring pin, etc. The bottom end of the limiting component 124 can be embedded in the limiting groove, thereby determining the relative position of the flow channel switching component 120 and the main body 110; secondly, the bottom end of the limiting component 124 can be embedded in the slide groove V3 and slide in the slide groove V3, thereby limiting the rotation angle range of the flow channel switching component 120 relative to the main body 110.

[0111] See also Figure 2e The limiting groove includes a first limiting groove V1 and a second limiting groove V2. When the limiting component 124 switches to the first limiting groove V1, the flow channel switching assembly 120 is located in the first position; when the limiting component 124 switches to the second limiting groove V2, the flow channel switching assembly 120 is located in the second position.

[0112] In the present disclosure, the limiting member 124 can move from the limiting groove into the chute V3 and slide along the chute V3, thereby switching the flow channel switching assembly 120 between the third position and the fourth position. It can be understood that due to the rotational motion of the flow channel switching assembly 120, the movement trajectory of the limiting member 124 is a circular trajectory, and accordingly, the chute V3 is a circular groove.

[0113] In the present disclosure, the limitation is achieved by the structure of the slide groove V3 itself. When the limiting component 124 slides to the end of the slide groove V3 close to the second limiting groove V2, the flow channel switching assembly 120 is located in the third position. When the limiting component slides to the end of the slide groove V3 close to the first limiting groove V1, the flow channel switching assembly 120 is located in the fourth position.

[0114] In an optional embodiment, the chute V3 is spliced ​​together by two chute sections. Specifically, the chute V3 includes a first chute section and a second chute section. The limiting component 124 is configured to slide along the first chute section to allow the flow channel switching assembly 120 to rotate to the third position. The limiting component 124 is configured to slide along the second chute section to allow the flow channel switching assembly 120 to rotate to the fourth position.

[0115] For example, the chute section close to the second limiting groove V2 is the first chute section, and the chute section close to the first limiting groove V1 is the second chute section. The limiting component 124 is a pre-loaded spring pin. The bottom walls of the first chute section and the second chute section are each provided with a shallow pit. The bottom end of the pre-loaded spring pin can pop out and embed into the shallow pit, generating a hysteresis during the rotation of the flow channel switching component 120, thereby allowing the user to determine that the flow channel switching component 120 is in the third position or the fourth position. Of course, when the rotational torque is increased, it is necessary to ensure that the pre-loaded spring pin can be moved out of the shallow pit.

[0116] In a preferred embodiment, the depth of the chute V3 is greater than the depths of the first limiting groove V1 and the second limiting groove V2. Taking the limiting component 124 as an example, if the chute V3 is sufficiently deep, the preloaded spring pin extends a long distance when located in the chute V3, preventing the preloaded spring pin from retracting and, consequently, from being removed from the chute V3. Thus, when the bottom end of the limiting component 124 is located in the chute V3, the flow channel switching assembly 120 can only switch between the third and fourth positions. In other words, after the flow channel switching assembly 120 switches from the first or second position to the chute V3, it cannot switch back to the first or second position. As previously mentioned, in the present disclosure, the microfluidic detection device 1000 has a first mode and a second mode. Through a properly designed limiting structure, the microfluidic detection device 1000 cannot switch from the second mode back to the first mode, thereby preventing user error.

[0117] It can be seen that in the present disclosure, the flow channel switching component 120 is essentially a five-position valve, which can switch between four gears by cooperating with the limiting structure to switch the connection and disconnection of different flow channels, thereby controlling the flow direction of the fluid.

[0118] Figure 3a for Figure 2a Schematic diagram of the flow channel switching component 120, Figure 3b for Figure 3a Exploded view of the flow channel switching assembly 120 in FIG. Figure 2c 、 Figure 2d 、 Figure 3a and Figure 3b The flow channel switching assembly 120 also includes a rotating body 121 and a rotating shaft 122. The main body 110 is provided with a rotating shaft mounting hole. The rotating shaft 122 can be inserted into the rotating shaft mounting hole. The rotating shaft 122 is loosely matched with the rotating shaft mounting hole, so that the rotating shaft 122 can rotate in the rotating shaft mounting hole.

[0119] Secondly, the two ends of the rotating shaft 122 are respectively connected to the rotating body 121 and the lower sealing plate 125. The upper sealing plate 123 and the limiting component 124 are connected to the rotating body 121. Because the upper sealing plate 123 is in contact with the body 110, the upper sealing plate 123 is located between the rotating body 121 and the body 110. It can be understood that the rotating body 121 is rotatably connected to the body 110 via the rotating shaft 122. When the rotating body 121 is twisted, the upper sealing plate 123, the limiting component 124, and the lower sealing plate 123 rotate accordingly.

[0120] In the present disclosure, the rotating body 121 is provided with a rotating shaft insertion hole K, into which the top end of the rotating shaft 122 can be inserted. To reduce the risk of relative rotation between the rotating shaft 122 and the rotating body 121, the rotating shaft insertion hole K is preferably a waist-shaped hole. Accordingly, the radial cross-section of the top end of the rotating shaft 122 is waist-shaped to facilitate insertion into the rotating shaft insertion hole K. Of course, the rotating shaft insertion hole K can also be a hole of other shapes, such as a rectangular hole, a triangular hole, or other non-circular hole, and this is not specifically limited here.

[0121] In an optional embodiment, the top end of the rotating shaft 122 and the rotating shaft insertion hole K can be interference fit, thereby achieving a fixed connection between the rotating shaft 122 and the rotating body 121. In the present disclosure, the top end of the rotating shaft 122 is fixedly connected to the rotating body 121 by a fastening connector (such as a screw or a pin).

[0122] In the present disclosure, a tray 1221 is provided at the bottom end of the rotating shaft 122, and the lower sealing plate 125 is connected to the tray 1221. Specifically, the lower sealing plate 125 can be placed onto the tray 1221 from the top end of the rotating shaft 122, and the lower sealing plate 125 is supported by the tray 1221. It can be understood that because the lower sealing plate 125 needs to be in contact with the body 110, the lower sealing plate 125 is located between the body 110 and the tray 1221. It should be noted that the upper sealing plate 123 and the rotating body 121, as well as the lower sealing plate 125 and the tray 1221, can be connected by heat fusion, ultrasonic welding, laser welding, gluing, etc.

[0123] In the present disclosure, the projection of the upper sealing plate 123 in the vertical direction at least covers the lower sealing plate 125. As previously mentioned, the sensing device 200 is connected to the bottom of the body 110. In order to leave more space for the installation of the sensing device 200, the lower sealing plate 125 is as small as possible while satisfying the layout of the liquid flow grooves. Although the number of liquid flow grooves arranged on the lower sealing plate 125 is greater than the number of liquid flow grooves on the upper sealing plate 123, the area of ​​the lower sealing plate 125 is still smaller than that of the upper sealing plate 123.

[0124] Figure 4a FIG. 1 is a partial exploded view of a microfluidic device 100 according to one embodiment of the present disclosure. Figure 4b for Figure 4a Another exploded view of the microfluidic device 100; Figure 4c for Figure 4b An exploded view of the microfluidic device 100 from another perspective. Figures 4a to 4c The main structure of the body 110 and the formation of each flow channel and the sealed chamber R in this disclosure are shown. Figures 4a to 4cIn the present disclosure, the main body 110 is a multi-plate assembly structure. Specifically, the main body 110 includes an upper plate 111, a flow channel plate 112 and a lower plate 113. In specific applications, the plates can be assembled by bonding, ultrasonic welding, laser welding or gluing.

[0125] Furthermore, flow channel grooves are provided on both the upper and lower surfaces of the flow channel plate 112. The upper plate 111 and lower plate 113 are disposed on the upper and lower sides of the flow channel plate 112, respectively, to form multiple flow channels together with the flow channel grooves. As can be understood, the multiple flow channels are arranged on the upper and lower sides of the flow channel plate 112, forming a double-layer flow channel structure. This significantly reduces the space occupied by the microfluidic device 100, achieving miniaturization.

[0126] Specifically, the flow channel grooves include a first flow channel groove G1 , a second flow channel groove G2 , a third flow channel groove G3 and a fourth flow channel groove G4 , for forming a first flow channel P1 , a second flow channel P2 , a third flow channel P3 and a fourth flow channel P4 , respectively.

[0127] See also Figures 4a to 4c In the present disclosure, the second flow channel opening C2 and the third flow channel opening C3 are disposed on the lower plate 113 , and the first flow channel opening C1 and the fourth flow channel opening C4 to the tenth flow channel opening C10 are disposed on the upper plate 111 .

[0128] In the present disclosure, the first flow channel G1 includes the first flow channel first section G11 and the first flow channel second section G12. The first flow channel first section G11 is located on the upper surface of the flow channel plate 112, and the first flow channel second section G12 is located on the lower surface of the flow channel plate 112. The first flow channel opening C1 is connected to the first flow channel first section G11, and the second flow channel opening C2 is connected to the first flow channel second section G12. The flow channel plate 112 is provided with a first through hole H1. The first through hole H1 connects the first flow channel first section G11 with the first flow channel second section G12 to form the first flow channel G1, and together form the first flow channel P1 when the upper plate 111 and the lower plate 113 cover the flow channel plate 112.

[0129] In the present disclosure, the third flow channel G3 includes a first section G31, a second section G32, and a third section G33. The first section G31 and the third section G33 are located on the upper surface of the flow channel plate 112, while the second section G32 is located on the lower surface of the flow channel plate 112. The sixth and seventh flow channel openings C6 and C7 are both connected to the first section G31, and the eighth flow channel opening C8 is connected to the third section G33.

[0130] The flow channel plate 112 is also provided with a third through hole H3 and a fourth through hole H4, which are respectively located at the two ends of the second section G32 of the third flow channel groove, thereby connecting the first section G31 of the third flow channel groove, the second section G32 of the third flow channel groove and the third section G33 of the third flow channel groove in sequence to form the third flow channel groove G3, and forming the third flow channel P3 together when the upper plate 111 and the lower plate 113 cover the flow channel plate 112.

[0131] As previously mentioned, in an optional embodiment, the third flow channel P3 is a circuitous and extending flow channel to facilitate fluid collection. Understandably, the third flow channel P3 occupies a relatively large space. Therefore, the third flow channel P3 is arranged on the upper and lower sides of the flow channel plate 112, resulting in a stacked structure that facilitates miniaturization.

[0132] In the present disclosure, the fourth flow channel G4 is a single flow channel segment and is located on the upper surface of the flow channel plate 112. The ninth flow channel opening C9 and the tenth flow channel opening C10 are both connected to the fourth flow channel G4, and together form the fourth flow channel P4 when the upper plate 111 covers the flow channel plate 112.

[0133] In the present disclosure, the second flow channel G2 includes the second flow channel first section G21, the second flow channel second section G22 and the second flow channel third section G23. The second flow channel first section G21 and the second flow channel second section G22 are located on the lower surface of the flow channel plate 112, the second flow channel third section G23 is located on the upper surface of the flow channel plate 112, and the third flow channel opening C3 is connected to the second flow channel first section G21.

[0134] The first liquid outlet D1 and the second liquid outlet D2 are located on the lower plate 113. The flow channel plate 112 is also provided with a second through hole H2. The second through hole H2 is located at the end of the second section G22 of the second flow channel groove to connect the second section G22 of the second flow channel groove with the third section G23 of the second flow channel groove. When the upper plate 111 and the lower plate 113 cover the flow channel plate 112, they form the second section P232 of the second flow channel together. The first section G21 of the second flow channel groove cooperates with the lower plate 113 to form the first section P231 of the second flow channel.

[0135] Furthermore, the second flow channel G2 also includes a second flow channel fourth section G24 and a second flow channel fifth section G25. The second flow channel fourth section G24 and the second flow channel fifth section G25 are both located on the upper surface of the flow channel plate 112. The fourth flow channel opening C4 is connected to the second flow channel fourth section G24, and the fifth flow channel opening C5 is connected to the second flow channel fifth section G25.

[0136] The second flow channel fourth section G24 and the second flow channel fifth section G25 are both connected to the second flow channel third section G23 and form the second flow channel first branch P21 and the second flow channel second branch P22 with the upper plate 111 respectively.

[0137] Furthermore, the first section G21 of the second flow channel groove is provided with a first buffer zone G211, while the second section G22 of the second flow channel groove is provided with a second buffer zone G221. The first buffer zone G211 is configured to gradually expand from the side away from the first liquid passage D1 toward the side closer to the first liquid passage D1; the second buffer zone G221 is configured to gradually expand from the side away from the second liquid passage D2 toward the side closer to the second liquid passage D2. In other words, the groove width is greater on the side closer to the liquid passage D2 than on the side away from the liquid passage D2. This reduces the fluid flow rate in the sensing area A1, facilitating sensing. In this disclosure, the first buffer zone G211 and the second buffer zone G221 are shaped as isosceles trapezoids, but this is not limiting and may also be semicircular, for example.

[0138] It should be noted that the number and arrangement positions of the groove sections in each flow channel groove can be adjusted according to the actual situation and are not specifically limited here.

[0139] See also Figures 4a to 4b In the present disclosure, a liquid reservoir L is provided on the lower surface of the flow channel plate 112. The liquid reservoir L is sealed by a membrane 140 and the lower plate 113 to form a sealed chamber R. In a specific application, a through opening is formed in the bottom wall of the flow channel buffer area A2 at a position corresponding to the fourth section G24 of the second flow channel groove to connect to the liquid reservoir L. The membrane 140 is sealed against the through opening. As can be seen, in the present disclosure, the membrane 140 is located between the liquid reservoir L and the flow channel buffer area A2 to separate the sealed chamber R from the fourth section G24 of the second flow channel groove, so that only ions can be exchanged between the fluid in the second section G22 of the second flow channel groove and the fluid in the sealed chamber R.

[0140] In a specific application, the lower plate 113 is provided with an opening for installing the conductive sheet 150, which overlaps with the liquid storage tank L in the up and down directions, that is, the opening is connected to the liquid storage tank L and is sealed by the conductive sheet 150, so that the conductive sheet 150 and the membrane 140 are both wetted by the fluid in the sealed chamber R.

[0141] See also Figure 4c The upper plate 111 is further provided with an eleventh flow channel opening C11 and a twelfth flow channel opening C12. The flow channel plate 112 is further provided with a fifth through hole H5 and a sixth through hole H6. The eleventh flow channel opening C11 is in communication with the liquid reservoir L via the fifth through hole H5, and the twelfth flow channel opening C12 is in communication with the liquid reservoir L via the sixth through hole H6. Both the eleventh flow channel opening C11 and the twelfth flow channel opening C12 are in communication with the sealed chamber R. It can be understood that the testing liquid is injected into the sealed chamber R through one of the eleventh flow channel opening C11 and the twelfth flow channel opening C12, while the other is used to expel air from the sealed chamber R. This ensures that the testing liquid is smoothly injected into the sealed chamber. It can be seen that the eleventh flow channel opening C11 and the twelfth flow channel opening C12 are used to inject the testing liquid.

[0142] See also Figure 2b In the present disclosure, the microfluidic device 100 further includes a sealing sticker 160 , which covers the eleventh flow channel opening C11 and the twelfth flow channel opening C12 , thereby preventing fluid leakage after pre-sealing the fluid in the sealed chamber R.

[0143] In the present disclosure, the sealed chamber R is formed by the liquid storage tank L, the membrane 140 and the lower plate 113, but it is of course not limited to this. For example, the sealed chamber R is formed in a sealed container provided with the membrane 140 and the conductive sheet 150. The sealed container is embedded in the main body 110 and can even be detachably connected to the main body 110. It can be seen that the structural form and shape of the sealed chamber R can be diverse, and no specific restrictions are made here.

[0144] It should be noted that in the present disclosure, the main body 110 is a structure in which three plate-like components are stacked and assembled in the vertical direction, but it is not limited to this. For example, in an optional embodiment, the main body 110 provided with various flow channels and a liquid storage tank L is made by an integrated molding process, for example, by 3D printing; then the membrane 140 and the conductive sheet 150 are assembled to seal the liquid storage tank L to form a sealed chamber R, and then the flow channel switching assembly 120, the fluid storage component 130 and the sealing sticker 160 are assembled. It can be understood that the production cost is relatively high under this method, especially the assembly of the membrane 140 is difficult. Alternatively, in another optional embodiment, the flow channel plate 112 and the upper plate 111 or the lower plate 113 are made by an integrated molding process, and the main body 110 is made by a two-plate assembly structure, thus avoiding the difficulty of assembling the membrane 140, but its production cost is relatively high. It can be seen that the structure of the main body 110 is not limited to this embodiment, and no specific restrictions are made here.

[0145] In actual applications, the observation area A3 is provided with openings in the projection position areas of the upper plate 111 and the flow channel plate 112, and the projection area of ​​the lower plate 113 is set as a transparent area, or the entire lower plate 113 is made of a transparent material (for example, a transparent acrylic plate, etc.), thereby forming the observation area A3 for convenient viewing of the sensing area A1.

[0146] It should be noted that in the present disclosure, the slide groove V3 is a quarter-circular arc groove, and the first limiting groove V1, the second limiting groove V2 and the two ends of the slide groove V3 are evenly spaced relative to the rotation center of the flow channel switching component 120. In this way, the angle between the two adjacent special positions and the line connecting the rotation center is 90°.

[0147] As mentioned above, when the flow channel switching assembly 120 is located at the first position, the limiting component 124 is located at the first limiting groove V1. Figure 6(a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid tank, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid tank when the limiting component 124 is located in the first limiting groove V1.

[0148] See also Figure 6 , the upper liquid troughs do not connect different flow channels, the fourth liquid trough B4 connects the second section G12 of the first flow channel and the first section G21 of the second flow channel. In this way, the fluid is injected through the first flow channel opening C1, and the fluid sequentially enters the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the fourth liquid trough B4, the first section G21 of the second flow channel, the sensing area A1 ( Figure 6 After the flow passes through the second flow channel groove second section G22, the second through hole H2 and the second flow channel groove third section G23, it is divided into the second flow channel groove fourth section G24 and the second flow channel groove fifth section G25.

[0149] The flow channel switching assembly 120 rotates counterclockwise by 90° on the basis of the first position, and the flow channel switching assembly 120 switches to the second position, and the limiting component 124 is located in the second limiting groove V2. Figure 7 (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid tank, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid tank when the limiting component 124 is located in the second limiting groove V2.

[0150] See also Figure 7 The first liquid channel B1 connects the fifth section G25 of the second flow channel with the first section G31 of the third flow channel, and the third liquid channel B3 connects the second section G12 of the first flow channel with the first section G21 of the second flow channel. In this way, the fluid is injected through the first flow channel opening C1 and flows through the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the third liquid channel B3, the first section G21 of the second flow channel, and the sensing area A1 ( Figure 7 After passing through the first liquid channel B1, it enters the first section G31 of the third flow channel trough, passes through the third through hole H3 and gradually fills the second section G32 of the third flow channel trough, and then enters the third section G33 of the third flow channel trough through the fourth through hole H4.

[0151] The flow channel switching assembly 120 rotates 90° counterclockwise on the basis of the second position, and the flow channel switching assembly 120 switches to the third position, and the limiting component 124 is located at one end of the sliding groove V3 close to the second limiting groove V2. Figure 8(a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid trough, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid trough when the limiting component 124 is located at one end of the slide groove V3 close to the second limiting groove V2.

[0152] See also Figure 8 The lower liquid trough does not connect different flow channels, and the second liquid trough B2 connects the first section G31 of the third flow channel with the fourth flow channel G4. It should be noted that in specific applications, the eighth flow channel opening C8 is a waste outlet, through which the fluid temporarily stored in the third flow channel G3 is extracted. The tenth flow channel opening C10 is a balance port used to connect to the atmosphere during liquid extraction. When waste liquid is extracted through the eighth flow channel opening C8, the fluid flows along the first section G31 of the third flow channel, the third through hole H3, the second section G32 of the third flow channel, the fourth through hole H4, and the third section G33 of the third flow channel, and out of the eighth flow channel opening C8.

[0153] The flow channel switching assembly 120 rotates 90° counterclockwise on the basis of the third position, and the flow channel switching assembly 120 switches to the fourth position, and the limiting component 124 is located at one end of the sliding groove V3 close to the first limiting groove V1. Figure 9 (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid trough, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid trough when the limiting component 124 is located at one end of the slide groove V3 close to the first limiting groove V1.

[0154] See also Figure 9 The second liquid channel B2 connects the fourth section G24 of the second flow channel with the first section G31 of the third flow channel. The fifth liquid channel B5 connects the second section G12 of the first flow channel with the first section G21 of the second flow channel. In this way, the fluid is injected through the first flow channel opening C1 and flows through the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the fifth liquid channel B5, the first section G21 of the second flow channel, the sensing area A1 ( Figure 9 After passing through the second liquid channel (not shown), the second section G22 of the second flow channel, the second through hole H2, the third section G23 of the second flow channel, the fourth section G24 of the second flow channel (including the flow buffer area A2) and the second liquid flow channel B2, the flow channel enters the first section G31 of the third flow channel, passes through the third through hole H3 and gradually fills the second section G32 of the third flow channel, and then passes through the fourth through hole H4 to enter the third section G33 of the third flow channel.

[0155] In the description of this specification, the reference terms "one embodiment," "some embodiments," "example," "specific example," or "some examples" mean that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0156] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0157] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A microfluidic device, characterized in that: The microfluidic device includes a body and a flow channel switching component; The main body is provided with a limiting structure and a plurality of flow channels; The flow channel switching assembly includes an upper sealing plate, a lower sealing plate, a limiting component and a rotating body. The upper sealing plate and the lower sealing plate are respectively attached to the upper and lower sides of the body. The upper sealing plate is provided with a plurality of upper liquid grooves, and the lower sealing plate is provided with a plurality of lower liquid grooves. The flow channel switching assembly is rotatably connected to the body, and the upper sealing plate, the lower sealing plate, and the limiting component can rotate relative to the body together with the rotating body, and the stopping position of the limiting component is determined by the limiting structure, so that the flow channel switching assembly can be switched between multiple positions to adjust the positions of the upper liquid trough and the lower liquid trough so that at least two of the multiple flow channels are connected through the upper liquid trough and / or the lower liquid trough; In which, when the flow channel switching component is in some of the multiple positions, it is in the first mode, and when the flow channel switching component is in another part of the multiple positions, it is in the second mode. The first mode can be switched to the second mode, and the second mode cannot be switched to the first mode.

2. The microfluidic device according to claim 1, wherein The plurality of flow channels include a first flow channel and a second flow channel, the second flow channel includes a second flow channel trunk, and the first flow channel and the second flow channel trunk are configured to be communicable through one of the plurality of lower liquid tanks.

3. The microfluidic device according to claim 2, wherein The second flow channel further includes a second flow channel first branch and a second flow channel second branch, and the second flow channel first branch and the second flow channel second branch intersect at the second flow channel main channel; The plurality of flow channels further include a third flow channel, and the third flow channel is configured to be able to communicate with the first branch of the second flow channel or the second branch of the second flow channel via one of the plurality of upper liquid troughs.

4. The microfluidic device according to claim 3, characterized in that The plurality of upper liquid passages include a first liquid passage and a second liquid passage; The plurality of lower liquid troughs include a third liquid trough, a fourth liquid trough, and a fifth liquid trough; The flow channel switching assembly is configured to rotate to a first position so that the fourth liquid passage connects the first flow channel with the second flow channel main trunk; The flow channel switching assembly is configured to rotate to a second position so that the third liquid channel connects the first flow channel with the second flow channel main channel, and the first liquid channel connects the second branch of the second flow channel with the third flow channel; The channel switching assembly is configured so that when it rotates to a fourth position, the third liquid passage connects the first channel and the second channel, and the second liquid passage connects the first branch of the second channel and the third channel.

5. The microfluidic device according to claim 4, characterized in that The plurality of flow channels further includes a fourth flow channel; The flow channel switching assembly is configured to be rotated to a third position so that the second liquid passage connects the third flow channel and the fourth flow channel.

6. The microfluidic device according to claim 5, characterized in that During the process of the flow channel switching assembly rotating relative to the body, the flow channel switching assembly switches among the first position, the second position, the third position and the fourth position.

7. The microfluidic device according to claim 6, characterized in that The limiting structure includes a first limiting groove, a second limiting groove and a sliding groove; When the limiting component is configured to be switched to the first limiting groove, the flow channel switching assembly is located at the first position; when the limiting component is configured to be switched to the second limiting groove, the flow channel switching assembly is located at the second position; The limiting component is configured to slide along the sliding groove to enable the flow channel switching component to switch between the third position and the fourth position.

8. The microfluidic device according to claim 7, characterized in that The chute includes a first chute section and a second chute section; The limiting component is configured to slide along the first chute section to enable the flow channel switching assembly to rotate to the third position; the limiting component is configured to slide along the second chute section to enable the flow channel switching assembly to rotate to the fourth position.

9. The microfluidic device according to claim 7, characterized in that The groove depth of the sliding groove is greater than the groove depths of the first limiting groove and the second limiting groove.

10. The microfluidic device according to claim 1, wherein The flow channel switching assembly further includes a rotating shaft, the rotating body is rotatably connected to the main body via the rotating shaft, the limiting component and the upper sealing plate are connected to the rotating body and can rotate with the rotating body; Both ends of the rotating shaft are respectively connected to the rotating body and the lower sealing plate, and the lower sealing plate can rotate along with the rotating shaft.

11. The microfluidic device according to claim 10, wherein: The rotating body is provided with a rotating shaft inserting hole, which is a waist-shaped hole. The radial cross-section of the top end of the rotating shaft is waist-shaped and can be inserted into the rotating shaft inserting hole.

12. The microfluidic device according to claim 10, wherein: A tray is provided at the bottom end of the rotating shaft, and the lower sealing plate is connected to the tray.

13. The microfluidic device according to claim 1, wherein The projection of the upper sealing plate in the vertical direction at least covers the lower sealing plate.

14. The microfluidic device according to claim 2, wherein: The first flow channel includes a second flow channel opening provided on the lower surface of the body; The second flow channel trunk includes a third flow channel opening provided on the lower surface of the body; The second flow channel opening, the third flow channel opening and the lower sealing plate are overlapped in the vertical direction.

15. The microfluidic device according to claim 5, wherein: The first branch of the second flow channel includes a fourth flow channel opening provided on the upper surface of the body; The second branch of the second flow channel includes a fifth flow channel opening provided on the upper surface of the body; The third flow channel includes a sixth flow channel opening and a seventh flow channel opening provided on the upper surface of the body; The fourth flow channel includes a ninth flow channel opening provided on the upper surface of the body; The fourth flow channel opening, the fifth flow channel opening, the sixth flow channel opening, the seventh flow channel opening, and the ninth flow channel opening are arranged to overlap with the upper sealing plate in the vertical direction.

16. The microfluidic device according to claim 2, wherein: The first flow channel includes a first flow channel opening, and the first flow channel opening is located on one side of the flow channel switching component; The microfluidic device further includes a fluid storage component connected to the body and in communication with the first flow channel opening.

17. The microfluidic device according to claim 3, wherein: The third flow channel includes an eighth flow channel opening, which is located on one side of the flow channel switching component and is used to discharge the fluid in the third flow channel.

18. The microfluidic device according to claim 3, wherein: The third flow channel is a flow channel that extends in a circuitous manner and is used to collect fluid.

19. The microfluidic device according to claim 5, wherein: The fourth flow channel includes a tenth flow channel opening, and the tenth flow channel opening is located on one side of the flow channel switching component.

20. The microfluidic device according to claim 1, wherein The body comprises an upper plate, a flow channel plate and a lower plate, and the upper surface and the lower surface of the flow channel plate are both provided with flow channel grooves; The upper plate and the lower plate are respectively arranged on the upper and lower sides of the flow channel plate to form a plurality of flow channels together with the flow channel grooves.

21. A microfluidic detection device, characterized in that: The microfluidic detection device comprises a sensing device and a microfluidic device according to any one of claims 2 to 9 and 14 to 19; The sensing device is disposed at the second flow channel trunk and connected to the body, and the sensing device and the corresponding portion of the body forming the second flow channel trunk together form a sensing area; or, The microfluidic detection device comprises a sensing device and a microfluidic device according to any one of claims 1, 10 to 13 and 20; The plurality of flow channels include a first flow channel and a second flow channel, the second flow channel includes a second flow channel trunk, and the first flow channel and the second flow channel trunk are configured to be able to communicate through one of the plurality of lower liquid tanks; The sensing device is disposed at the second flow channel trunk and connected to the body, and the sensing device and the corresponding portion of the body forming the second flow channel trunk together form a sensing area.

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