A rapid detection method for Vibrio harveyi

Abstract

The invention relates to the technical field of Vibrio harveyi detection, and specifically to a method for rapid detection of Vibrio harveyi, comprising stabilization and taking out; the beneficial effects are as follows: a plurality of placement frames are arranged on the top of a base, the placement frames comprising a boiling water bath, a thermal cycle position and an observation position, and a culture dish can be quickly moved to a corresponding position, thereby speeding up the operation speed and not causing the spillage of reagents; the culture dish is placed on the surface of a placement plate, and a force-bearing plate moves with the force-bearing plate, so that the placement plate is retracted into a fixed frame, and a spring is held on the movable plate, so that the holding teeth are held on the surface of a pressing plate, and the pressing plate is held on the surface of the force-bearing plate, so that the placement plate is fixed and the culture dish is stabilized.

Description

Technical Field

[0001] The invention relates to the technical field of Vibrio harveyi detection, in particular to a method for rapid detection of Vibrio harveyi. Background Art

[0002] Vibrio disease is one of the most serious bacterial diseases that causes damage to fish, shrimp, and shellfish. The aquaculture industry often suffers huge economic losses due to it. It is a typical conditional pathogen. When the external water environment deteriorates and various factors cause the resistance of aquatic animals to decrease, pathogenic Vibrio will multiply rapidly, causing aquatic animals to be infected with Vibrio disease.

[0003] However, traditional Vibrio harveyi detection methods, such as selective culture medium and physiological and biochemical test detection, involve steps such as hot water baths and specific slice growth, and require the use of alcohol lamps, thermal cyclers and other equipment. During the test, the staff needs to carry the reagents back and forth between these devices, which is very physically demanding, and the reagents are prone to spillage when moving, affecting the test results. Summary of the invention

[0004] The object of the present invention is to provide a rapid detection method for Vibrio harveyi to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for rapid detection of Vibrio harveyi, comprising:

[0006] Step 1:

[0007] Stable, multiple groups of placement frames are arranged on the top of the base, and the placement frames include a boiling water bath, a thermal cycle position and an observation position, which can quickly move the culture dish to the corresponding position, speeding up the operation and not causing the reagent to spill. The culture dish is placed on the surface of the placement plate, and the force plate moves with the force plate, so that the placement plate is retracted into the fixed frame, and the spring is held on the moving plate, so that the holding teeth are held on the surface of the extrusion plate, and the extrusion plate is held on the surface of the force plate, so that the placement plate is fixed and the culture dish is stabilized;

[0008] Step 2:

[0009] When the locking cam is in the locked position, the locking cam is released and the locking cam is in the locked position, so that the locking cam can be moved to the locking position on the locking cam.

[0010] Preferably, a column is provided on the top of the base; an arc rod is provided at the end of the load-bearing block, a connecting rod is provided at the end of the arc rod, a movable plate is provided at the bottom of the connecting rod, a top holding tooth is provided at the bottom of the movable plate, an extrusion plate is provided at the bottom of the movable plate, and a groove is provided on the surface of the extrusion plate; and a placement frame is provided on the top of the base.

[0011] Preferably, the placement frame is provided with multiple groups, the column is fixed on the top of the base, the top of the column is inserted with a limit rod, the surface of the limit rod is provided with a limit strip, the inner wall of the column is provided with a limit groove, the limit strip is inserted in the limit groove, the limit rod can move inside the column, and the limit strip moves with the limit rod.

[0012] Preferably, a second rotating ring is fixed to the top of the limiting rod, and the second rotating ring rotates and moves along with the limiting rod. A rotating shaft is inserted into the top of the second rotating ring, and the end of the rotating shaft is located inside the column. The rotating shaft can rotate inside the limiting rod, and a first rotating ring and a top holding block are fixed to the top of the rotating shaft, and the first rotating ring and the top holding block rotate along with the rotating shaft.

[0013] Preferably, a support rod is fixed to the surface of the limit rod, and the end of the support rod is connected to the fixed frame. The support rod rotates along with the limit rod. A force-bearing plate is inserted into the surface of the fixed frame, and the force-bearing plate can move inside the fixed frame. A placement plate is fixed to the end of the force-bearing plate, and a culture dish is placed on the top of the placement plate.

[0014] Preferably, a movable plate is fixed to the end of the connecting rod, and the movable plate is inserted into the surface of the fixed frame. A groove is opened on the surface of the fixed frame, and the movable plate is located in the groove and can move in the groove. A spring is connected to the end of the movable plate, and a plurality of groups of top holding teeth are fixed to the surface of the movable plate.

[0015] Preferably, the extrusion plate is located inside the fixed frame, and the extrusion plate can move inside the fixed frame. After the extrusion plate moves, it is held on the surface of the force-bearing plate, and the holding teeth are held on the surface of the extrusion plate. The holding teeth are held in the groove after the extrusion plate moves.

[0016] Preferably, a supporting plate is fixed to the surface of the rotating shaft, and the supporting plate is located inside the column. An extension rod is fixed to the bottom of the limiting rod, and the extension rod is located inside the column. The extension rod rotates with the limiting rod. After the extension rod rotates, it is located on the top of the supporting plate, and the supporting plate is supported on the bottom of the extension rod.

[0017] Preferably, a slot is provided at the bottom of the limiting rod, and when the limiting rod is inserted into the interior of the column, the supporting plate is inserted into the interior of the slot.

[0018] Preferably, the supporting block is supported on the surface of the load-bearing block as the rotating shaft rotates, the load-bearing block moves under the force, and the arc rod moves along with the load-bearing block.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The top of the base proposed by the present invention is provided with multiple groups of placement frames, which include a boiling water bath, a thermal cycle position and an observation position, and can quickly move the culture dish to the corresponding position, thereby speeding up the operation speed and not causing the reagent to spill. The culture dish is placed on the surface of the placement plate, and the force-bearing plate moves with the force-bearing plate, so that the placement plate is retracted into the fixed frame, and the spring is supported on the moving plate, so that the supporting teeth are supported on the surface of the extrusion plate, and the extrusion plate is supported on the surface of the force-bearing plate, so that the placement plate is fixed and the culture dish is stabilized. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0022] Figure 2 It is a structural schematic diagram of another viewing angle of the present invention;

[0023] Figure 3 It is a schematic diagram of the cross-sectional structure of the present invention;

[0024] Figure 4 It is a structural schematic diagram of another viewing angle of the cross section of the present invention;

[0025] Figure 5 This is a schematic diagram of the arc rod structure of the present invention;

[0026] Figure 6 It is a schematic diagram of a partially enlarged cross-section structure of the present invention;

[0027] Figure 7 This is a structural schematic diagram of another viewing angle of a partially enlarged cross section of the present invention.

[0028] In the figure: base 1, placement frame 2, column 3, first rotating ring 4, holding block 5, rotating shaft 6, second rotating ring 7, limit rod 8, limit bar 9, fixed frame 10, force plate 11, placement plate 12, arc rod 13, connecting rod 14, support rod 15, load block 16, holding teeth 17, movable plate 18, extrusion plate 19, groove 20, spring 21, support plate 22, extension rod 23, slot 24. DETAILED DESCRIPTION

[0029] In order to make the purpose and technical solution of the present invention clearly and completely described, and the advantages more clearly understood, the embodiments of the present invention are further described in detail with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are part of the embodiments of the present invention, rather than all of the embodiments, and are only used to explain the embodiments of the present invention, and are not used to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] See also Figures 1 to 7 The present invention provides a technical solution: a column 3 is arranged on the top of the base 1; an arc rod 13 is arranged on the end of the load-bearing block 16, a connecting rod 14 is arranged on the end of the arc rod 13, a movable plate 18 is arranged on the bottom of the connecting rod 14, a top holding tooth 17 is arranged on the bottom of the movable plate 18, an extrusion plate 19 is arranged on the bottom of the movable plate 18, and a groove 20 is arranged on the surface of the extrusion plate 19; and a placement frame 2 is arranged on the top of the base 1, and the placement frame 2 is provided with multiple groups, the column 3 is fixed on the top of the base 1, the top of the column 3 is plugged with a limiting rod 8, the surface of the limiting rod 8 is provided with a limiting strip 9, the inner wall of the column 3 is provided with a limiting groove, the limiting strip 9 is plugged into the limiting groove, the limiting rod 8 can move inside the column 3, the limiting strip 9 moves with the limiting rod 8, and a second rotating member is fixed on the top of the limiting rod 8 Ring 7, the second rotating ring 7 rotates and moves with the limiting rod 8, the top of the second rotating ring 7 is plugged with a rotating shaft 6, the end of the rotating shaft 6 is located inside the column 3, the rotating shaft 6 can rotate inside the limiting rod 8, the top of the rotating shaft 6 is fixed with a first rotating ring 4 and a top holding block 5, the first rotating ring 4 and the top holding block 5 rotate with the rotating shaft 6, the surface of the limiting rod 8 is fixed with a supporting rod 15, the end of the supporting rod 15 is connected to the fixed frame 10, the supporting rod 15 rotates with the limiting rod 8, the limiting rod 8 is pulled up, so that the fixed frame 10 moves with the limiting rod 8, when the limiting bar 9 is no longer plugged into the inside of the column 3, the limiting rod 8 can be rotated, so that the fixed frame 10 moves with the limiting rod 8, the fixed frame 10 is moved to the top of another group of placement frames 2, and the position of the limiting rod 8 is maintained;

[0031] A force-bearing plate 11 is plugged into the surface of the fixed frame 10, and the force-bearing plate 11 can move inside the fixed frame 10. A placement plate 12 is fixed to the end of the force-bearing plate 11, and a culture dish is placed on the top of the placement plate 12. A movable plate 18 is fixed to the end of the connecting rod 14, and the movable plate 18 is plugged into the surface of the fixed frame 10. A groove is opened on the surface of the fixed frame 10, and the movable plate 18 is located in the groove and can move in the groove. A spring 21 is connected to the end of the movable plate 18. A plurality of groups of top holding teeth 17 are fixed to the surface of the movable plate 18. A pressing plate 19 is located inside the fixed frame 10, and the pressing plate 19 can move inside the fixed frame 10. After the pressing plate 19 moves, it is held on the surface of the force-bearing plate 11, and the top holding teeth 17 are held on the surface of the pressing plate 19. The top holding teeth 17 are held in the groove 20 as the pressing plate 19 moves. A supporting plate 22 is fixed to the surface of the rotating shaft 6, and the supporting plate 22 is located inside the column 3. An extension rod 23 is fixed to the bottom of the limiting rod 8, and the extension rod 23 is located inside the column 3. The extension rod 23 rotates with the limiting rod 8. After the extension rod 23 rotates, it is located at the top of the supporting plate 22. The supporting plate 22 is supported on the bottom of the extension rod 23. A slot 24 is provided at the bottom of the limiting rod 8. When the limiting rod 8 is inserted into the interior of the column 3, the supporting plate 22 is inserted into the interior of the slot 24. After the rotating shaft 6 rotates, the supporting block 5 is supported on the surface of the load-bearing block 16. The load-bearing block 16 moves under the force, and the arc rod 13 moves with the load-bearing block 16. After the supporting block 5 rotates, it is supported on the surface of the load-bearing block 16, so that the arc rod 13 moves, and the arc rod 13 drives the movable plate 18 to move, and the spring 21 is compressed.

[0032] A plurality of placement frames 2 are arranged on the top of the base 1. The placement frames 2 include a boiling water bath, a thermal cycle position and an observation position, which can quickly move the culture dish to the corresponding position, speeding up the operation and not causing the reagent to spill. The culture dish is placed on the surface of the placement plate 12, and the force plate 11 moves with the force plate 11, so that the placement plate 12 is retracted into the fixed frame 10, and the spring 21 is supported on the movable plate 18, so that the top holding teeth 17 are supported on the surface of the squeezing plate 19, and the squeezing plate 19 is supported on the surface of the force plate 11 to fix the placement plate 12 and stabilize the culture dish. The fixed frame 10 moves with the limiting rod 8, and the fixed frame 10 can be placed in the placement frame 2. When the fixed frame 10 needs to be moved, the limiting rod 8 is pulled up so that the fixed frame 10 moves with the limiting rod 8. When the limiting bar 9 is no longer plugged into the inside of the column 3, the limiting rod 8 can be rotated so that The fixed frame 10 moves with the limiting rod 8, moves the fixed frame 10 to the top of another group of placement frames 2, and maintains the position of the limiting rod 8, rotates the first rotating ring 4, so that the rotating shaft 6 rotates, and after the rotating shaft 6 rotates, the supporting plate 22 rotates to the bottom of the extension rod 23, so that the height of the limiting rod 8 is maintained at a different level. In the process of rotating the first rotating ring 4, the supporting block 5 rotates with the first rotating ring 4. After the supporting block 5 rotates, it is supported on the surface of the load-bearing block 16, so that the arc rod 13 moves, and the arc rod 13 drives the moving plate 18 to move. The spring 21 is compressed, and after the moving plate 18 moves, the supporting tooth 17 moves into the groove 20, and the squeezing plate 19 moves up inside the fixed frame 10, so that the squeezing plate 19 is no longer supported on the surface of the force-bearing plate 11, so that the position of the placement plate 12 can be moved, and the culture dish is taken out.

[0033] Although the above describes the illustrative specific implementation methods of the present application so that technicians in this technical field can understand the present application, the present application is not limited to the scope of the specific implementation methods. For ordinary technicians in this technical field, as long as various changes are within the spirit and scope of the present application defined and determined by the attached claims, all application creations using the concept of the present application are protected.

Claims

1. A rapid detection method for Vibrio harveyi for non-diagnostic purposes, characterized in that: include: Step 1: Stability. A plurality of placement frames (2) are arranged on the top of the base (1). The placement frames (2) include a boiling water bath, a thermal cycle position and an observation position. The culture dish can be quickly moved to a corresponding position, which speeds up the operation and does not cause the reagent to spill. The culture dish is placed on the surface of the placement plate (12). The placement plate (12) moves along with the force-bearing plate (11), so that the placement plate (12) is retracted into the fixed frame (10), and the spring (21) is supported on the movable plate (18), so that the supporting teeth (17) are supported on the surface of the squeezing plate (19), and the squeezing plate (19) is supported on the surface of the force-bearing plate (11), so that the placement plate (12) is fixed and the culture dish is stabilized; Step 2: Take out, the fixed frame (10) moves with the limiting rod (8), and the fixed frame (10) can be placed in the placement frame (2). When the fixed frame (10) needs to be moved, pull up the limiting rod (8) so that the fixed frame (10) moves with the limiting rod (8). When the limiting bar (9) is no longer inserted into the inside of the column (3), the limiting rod (8) can be rotated so that the fixed frame (10) moves with the limiting rod (8), and the fixed frame (10) is moved to the top of another group of placement frames (2), and the position of the limiting rod (8) is maintained. The first rotating ring (4) is rotated to rotate the rotating shaft (6). After the rotating shaft (6) rotates, the supporting plate (22) is rotated to the extension The bottom of the extension rod (23) keeps the height of the limit rod (8) stationary. During the rotation of the first rotating ring (4), the supporting block (5) rotates along with the first rotating ring (4). After the supporting block (5) rotates, it supports the surface of the load-bearing block (16), so that the arc rod (13) moves. The arc rod (13) drives the movable plate (18) to move. The spring (21) is compressed. After the movable plate (18) moves, the supporting teeth (17) move into the groove (20). The pressing plate (19) moves upward inside the fixed frame (10), so that the pressing plate (19) is no longer supported on the surface of the load-bearing plate (11), so that the position of the placement plate (12) can be moved. The top of the base (1) is provided with a column (3); the end of the load-bearing block (16) is provided with an arc rod (13), the end of the arc rod (13) is provided with a connecting rod (14), the bottom of the connecting rod (14) is provided with a moving plate (18), the bottom of the moving plate (18) is provided with a top holding tooth (17), the bottom of the moving plate (18) is provided with an extrusion plate (19), and the surface of the extrusion plate (19) is provided with a groove (20); and the placement frame (2) is arranged on the top of the base (1); The placement frame (2) is provided with multiple groups, the column (3) is fixed on the top of the base (1), the top of the column (3) is plugged with a limit rod (8), the surface of the limit rod (8) is provided with a limit strip (9), the inner wall of the column (3) is provided with a limit groove, the limit strip (9) is plugged into the limit groove, the limit rod (8) can move inside the column (3), and the limit strip (9) moves with the limit rod (8); A second rotating ring (7) is fixed on the top of the limiting rod (8), and the second rotating ring (7) rotates and moves along with the limiting rod (8). A rotating shaft (6) is inserted on the top of the second rotating ring (7), and the end of the rotating shaft (6) is located inside the column (3). The rotating shaft (6) can rotate inside the limiting rod (8). A first rotating ring (4) and a top holding block (5) are fixed on the top of the rotating shaft (6), and the first rotating ring (4) and the top holding block (5) rotate along with the rotating shaft (6); A support rod (15) is fixed on the surface of the limiting rod (8), the end of the support rod (15) is connected to the fixed frame (10), the support rod (15) rotates along with the limiting rod (8), a force-bearing plate (11) is plugged into the surface of the fixed frame (10), the force-bearing plate (11) can move inside the fixed frame (10), a placement plate (12) is fixed at the end of the force-bearing plate (11), and a culture dish is placed on the top of the placement plate (12); A movable plate (18) is fixed at the end of the connecting rod (14), and the movable plate (18) is plugged into the surface of the fixed frame (10). A groove is provided on the surface of the fixed frame (10), and the movable plate (18) is located in the groove and can move in the groove. A spring (21) is connected to the end of the movable plate (18), and a plurality of groups of top holding teeth (17) are fixed on the surface of the movable plate (18); The extrusion plate (19) is located inside the fixed frame (10), and the extrusion plate (19) can move inside the fixed frame (10). After the extrusion plate (19) moves, it is held against the surface of the force-bearing plate (11), and the holding teeth (17) are held against the surface of the extrusion plate (19). After the extrusion plate (19) moves, the holding teeth (17) are held in the groove (20); A support plate (22) is fixed on the surface of the rotating shaft (6), the support plate (22) is located inside the column (3), an extension rod (23) is fixed on the bottom of the limiting rod (8), the extension rod (23) is located inside the column (3), the extension rod (23) rotates along with the limiting rod (8), and the extension rod (23) is located on the top of the support plate (22) after rotation, and the support plate (22) is supported on the bottom of the extension rod (23); The bottom of the limiting rod (8) is provided with a slot (24), and when the limiting rod (8) is inserted into the interior of the column (3), the supporting plate (22) is inserted into the interior of the slot (24); The supporting block (5) is supported on the surface of the load-bearing block (16) as the rotating shaft (6) rotates, and the load-bearing block (16) moves under the force, and the arc rod (13) moves along with the load-bearing block (16).

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