Automatic pouring and inoculating workstation for laboratory microorganisms

Abstract

The utility model belongs to the technical field of automatic control, and particularly relates to an automatic pouring and inoculating workstation for laboratory microorganisms. The work station comprises a sample manipulator for grabbing a sample bottle to move; the weighing sensor module is used for weighing the sample bottle; the diluent filling mechanism is used for filling a diluent into the sample bottle; the vibrating and uniformly mixing mechanism is used for vibrating and uniformly mixing the sample; the pipetting module is used for driving a pipetting gun head to move and carrying out quantitative pipetting operation; the culture dish manipulator grabs the culture dish to move; the culture dish rotating movement mechanism drives the culture dish to rotate and move; the culture medium filling mechanism is used for quantitatively filling a culture medium into the culture dish; and the culture medium covering temporary storage mechanism is used for filling the culture medium into the culture dish for covering liquid covering treatment. The workstation can be used for automatically pouring and inoculating microorganisms, and the problem of low efficiency of microbiological inspection is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of automatic control, and in particular relates to an automatic pouring and inoculation workstation for laboratory microorganisms. Background Art

[0002] Currently, when conducting microbiological tests in laboratories, from preliminary preparation to sample pouring inoculation method (definition: the sample to be tested is placed in liquid form in a culture dish, and then poured into the culture medium cooled to about 45°C, and quickly and gently shaken to disperse the sample with the melted culture medium. After the culture medium condenses, the dispersed sample is fixed in place to form colonies.) The entire pouring method process is complicated and the steps are tedious. The process uses a large number of consumables and equipment, which makes high-throughput operations impossible, ultimately resulting in low efficiency of microbiological testing. Utility Model Content

[0003] The utility model provides a laboratory microorganism automatic pouring and inoculation workstation, which can perform automatic microorganism pouring and inoculation operations and solve the problem of low efficiency of microbiological testing.

[0004] The technical solution of the present utility model is achieved as follows:

[0005] A laboratory microbial automated pouring inoculation workstation includes a clean bench, wherein the following components are installed inside the clean bench:

[0006] Sample manipulator, used to grab sample bottles and move them;

[0007] A weighing sensor module for weighing the sample bottle;

[0008] A diluent filling mechanism, used for adding diluent into a sample bottle containing a sample;

[0009] Metal bath heating module, used to heat the sample bottle with diluent added;

[0010] A vibration mixing mechanism, used for vibrating and mixing the sample in the sample bottle;

[0011] The pipetting module is used to drive the pipette tip to move and perform quantitative liquid aspiration operations;

[0012] A culture dish manipulator, used to grab the culture dish and move it;

[0013] The culture dish rotation mechanism is used to drive the culture dish to rotate;

[0014] A culture medium filling mechanism is used to quantitatively fill the culture medium into the culture dish;

[0015] The culture dish shaking gripper mechanism is used to grab the culture dish cover and shake and mix the culture dish filled with culture medium;

[0016] The culture medium covering temporary storage mechanism is used to add culture medium into the culture dish for covering liquid covering treatment.

[0017] Optionally, a sample loading bracket and an empty container bracket are installed inside the clean bench. The sample loading bracket is used to hold sample bottles filled with samples, and the empty container bracket is used to hold empty sample bottles.

[0018] Optionally, an automatic code scanning module and a rotary cover opening mechanism are installed inside the clean bench. The automatic code scanning module is used to scan and identify the sample bottles, and the rotary cover opening mechanism is used to rotary open the caps of the sample bottles grasped by the sample manipulator.

[0019] Optionally, a gun tip loading mechanism is installed inside the clean workbench, and the gun tip loading mechanism is located below the pipetting module. A pipette gun is installed at the lower end of the pipetting module. The gun tip loading mechanism provides a pipette tip for the pipette gun in the pipetting module. An export plate is installed on the front side of the gun tip loading mechanism. The cross-section of the export plate is L-shaped, and the export plate is continuously provided with several positioning holes along its own length direction, and a pipette tip is vertically inserted into the positioning hole.

[0020] Optionally, a waste collection module is installed inside the clean bench, and the waste liquid collection module is located at the front side of the tip loading mechanism. The waste collection module is used to collect the pipette tip after the aspiration is completed.

[0021] Optionally, a driving device is installed inside the clean bench, and the driving device drives the pipetting module to move toward the vibration mixing mechanism to perform a quantitative liquid aspiration operation, the driving device drives the pipetting module to move toward the empty sample bottle at the weighing sensor module to perform a liquid discharge operation, and the driving device drives the pipetting module to move toward the waste collection module to perform a pipette tip discharge operation;

[0022] The driving device includes three linear modules, two of which are arranged in parallel and spaced apart in front and back, and the third linear module is located on top of the two linear modules and fixedly connected to the two sliders of the two linear modules. The slider of the third linear module is fixedly connected to the pipetting module.

[0023] Optionally, a culture dish rotary tower loading mechanism and a culture dish coding module are installed inside the clean workbench. The culture dish rotary tower loading mechanism is used to hold empty culture dishes, and the culture dish coding module is used to code the culture dishes grasped by the culture dish robot.

[0024] Optionally, the culture dish rotating movement mechanism comprises a rotating disc, a plurality of limiting holes are formed in the top of the rotating disc in a circumferential direction, the diameter of the limiting hole is smaller than the diameter of the culture dish, a jacking cylinder is arranged below the rotating disc, the piston rod of the jacking cylinder is vertically upward, a jacking plate is fixedly connected to the end of the piston rod of the jacking cylinder, and the jacking plate is pushed upward by the jacking cylinder.

[0025] Optionally, the top of the rotating disc is provided with a culture dish shaking clamping jaw mechanism, the culture dish shaking clamping jaw mechanism comprises a mounting seat, a clamping cylinder, a motor and a shaking arm, the motor is vertically mounted on the top of the mounting seat, the shaking arm is slidingly connected to the bottom of the mounting seat, the clamping cylinder is mounted on the end of the shaking arm, the output shaft of the motor is rotationally connected to the top of the clamping cylinder through a cam, and two clamping jaws of the clamping cylinder A are respectively fixedly connected with arc-shaped clamping plates, the two arc-shaped clamping plates are oppositely arranged and surround a circle, and the two clamping jaws A of the clamping cylinder A are used to drive the two arc-shaped clamping plates to clamp the cover of the culture dish.

[0026] After the above technical scheme is adopted, the working station has the following beneficial effects:

[0027] The working station in the utility model does not need repeated operation, has high automation degree, and is automatically processed, the risk of bacterial contamination caused by personnel operation is avoided in the microbial pouring inoculation process, repeated manual labor is reduced, and the efficiency and quality of pouring inoculation in microbial inspection are improved. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained without creative labor under the premise of the drawings.

[0029] Figure 1 It is the structure schematic diagram of the working station in the embodiment Figure I ;

[0030] Figure 2 It is the structure schematic diagram of the working station in the embodiment Figure II ;

[0031] Figure 3 It is the structure schematic diagram of the working station in the embodiment Figure III ;

[0032] Figure 4 It is the structure schematic diagram of the working station in the embodiment Figure IV ;

[0033] Figure 5 This is a schematic diagram of the structure of the culture dish rotation mechanism in the embodiment. Figure I ;

[0034] Figure 6 This is a schematic diagram of the structure of the culture dish rotation mechanism in the embodiment. Figure II ;

[0035] Figure 7 This is a schematic diagram of the structure of the culture dish rotation mechanism in the embodiment. Figure III ;

[0036] Figure 8 This is a schematic structural diagram of the gun head feeding mechanism in the embodiment;

[0037] Figure 9 Schematic diagram of the structure of the driving device in the embodiment.

[0038] Explanation of the accompanying symbols: 1. Clean bench; 2. Sample manipulator; 3. Weighing sensor module; 4. Dilution filling mechanism; 5. Metal bath heating module; 6. Vibration mixing mechanism; 7. Pipetting module; 8. Culture dish manipulator; 9. Culture dish rotation mechanism; 10. Culture medium filling mechanism; 11. Culture dish shaking clamp mechanism; 12. Culture medium covering temporary storage mechanism; 13. Sample loading bracket; 14. Empty container bracket; 15. Automatic code scanning module; 16. Rotation Opening mechanism; 17. Gun head loading mechanism; 18. Pipette gun; 19. Export plate; 20. Waste collection module; 21. Petri dish rotary tower loading mechanism; 22. Petri dish coding module; 23 Turntable; 24. Lifting cylinder; 25. Lifting plate; 26. Clamping cylinder A; 27. Chuck A; 28. Arc clamping plate; 29. ​​Linear module; 30. Slider; 31. Motor; 32. Rocking arm; 33. Column; 34. Support plate; 35. U-shaped block; 36. Cam. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0040] The embodiment of the present application discloses a laboratory microbial automated pouring inoculation workstation.

[0041] Example

[0042] according to Figures 1 to 9As shown, a laboratory microbial automated pouring inoculation workstation includes three clean benches 1 and an automation system. The clean bench 1 is a prior art and is designed to adapt to automated operations. The clean bench 1 has an ultraviolet disinfection lamp and a lighting system inside. After it is turned on, the interior needs to be subjected to ultraviolet disinfection treatment for no less than 30 minutes to ensure internal cleanliness. The automation system is a prior art and is used to control the operation of the entire workstation and store information.

[0043] The first clean bench 1 is internally provided with a sample loading bracket 13, an empty container bracket 14, a sample manipulator 2, an automatic code scanning module 15, a rotary lid opening mechanism 16, a weighing sensor module 3, and a diluent filling mechanism 4. The sample manipulator 2 is located around the sample loading bracket 13, the empty container bracket 14, the weighing sensor module 3, and the diluent filling mechanism 4. The sample manipulator 2 is located between the weighing sensor module 3 and the diluent filling mechanism 4. The automatic code scanning module 15 and the rotary lid opening mechanism 16 are installed on the inner wall of the first clean bench 1. The sample loading bracket 13, the empty container bracket 14, the sample manipulator 2, the automatic code scanning module 15, the rotary lid opening mechanism 16, the weighing sensor module 3, and the diluent filling mechanism 4 all belong to the prior art, and the specific structure and implementation principle are not repeated here.

[0044] A sample loading bracket 13 is used to hold sample bottles containing samples;

[0045] an empty container holder 14 for holding empty sample bottles;

[0046] Sample manipulator 2, used to grab the sample bottle and move it;

[0047] Automatic code scanning module 15, used to scan and identify the sample bottle, thereby matching the relevant information of the sample;

[0048] The rotary opening mechanism 16 is used to open the sample bottle grasped by the sample manipulator 2 by rotating it;

[0049] Weighing sensor module 3, used for weighing empty sample bottles and sample bottles containing samples;

[0050] The diluent filling mechanism 4 is used to add diluent into the sample bottle containing the sample.

[0051] The second clean bench 1 is internally equipped with a vibration mixing mechanism 6, a metal bath heating module 5, a gun tip loading mechanism 17, a pipetting module 7, a driving device, a waste collection module 20, a culture medium filling mechanism 10 and a culture dish rotating motion mechanism 9. The vibration mixing mechanism 6 and the metal bath heating module 5 are located at one end of the second clean bench 1 close to the first clean bench 1, the gun tip loading mechanism 17 is located on one side of the metal bath heating module 5, the pipetting module 7 and the driving device are located above the gun tip loading mechanism 17, the waste liquid collection module is located in front of the gun tip loading mechanism 17, the culture medium filling mechanism 10 is located on one side of the gun tip loading mechanism 17, and the culture dish rotating motion mechanism 9 is located in front of the culture medium filling mechanism 10. The vibration mixing mechanism 6, the gun tip loading mechanism 17, the pipetting module 7, the driving device, the waste collection module 20, the metal bath heating module 5, the culture medium filling mechanism 10 and the culture dish rotating motion mechanism 9 belong to the prior art, and the specific structure and implementation principle are not repeated here.

[0052] The metal bath heating module 5 is used to heat or not heat the sample bottle with the diluent added;

[0053] The vibration mixing mechanism 6 is used to perform vibration mixing on the sample grabbed by the sample manipulator 2;

[0054] The tip loading mechanism 17 is used to provide the pipette tips for the pipetting module 7. The pipette tips are used to enter the sample bottle for aspiration processing. The front side of the tip loading mechanism 17 is equipped with an output plate 19. The output plate 19 has an L-shaped cross section. The output plate 19 is continuously provided with a plurality of positioning holes along its length. The positioning holes are vertically inserted into the pipette tips.

[0055] A pipette gun 18 is installed at the lower end of the pipetting module 7, and a driving device is used to drive the pipetting module 7 to move. The pipetting module 7 drives the pipette gun head to move to the sample bottle that needs to be aspirated in the vibration mixing mechanism 6 to perform quantitative aspiration. After the aspiration is completed, the driving device drives the pipette module 7 to move to the empty sample bottle on the weighing sensor module 3 for drainage. After drainage, the driving device drives the pipette module 7 to move to the waste collection module 20 to discharge the used pipette gun head to prevent secondary contamination; the pipetting module 7 and the pipette gun 18 belong to the prior art, and the specific structure and the principle of realizing automatic quantitative sampling belong to the prior art, which will not be repeated here.

[0056] The drive device includes three linear modules 29, two of which are arranged parallel to each other in a front-to-back manner, and the third linear module 29 is located on top of the two linear modules 29 and is fixedly connected to the two sliders 30 of the two linear modules 29. The slider 30 of the third linear module is fixedly connected to the pipetting module 7. The side of the pipetting module 7 is equipped with a pipette gun 18, which is placed vertically. The first two linear modules 29 drive the third linear module 29 to move horizontally, and the third linear module 29 can drive the pipette gun 18 to move in the front-to-back direction. The three linear modules 29 drive the pipette gun 18 to move in the left-right direction and the front-to-back direction. The linear module 29 belongs to the existing technology, and the specific structure and working principle will not be described in detail here.

[0057] Waste collection module 20, used to collect pipette tips after aspiration;

[0058] The culture dish rotation motion mechanism 9 is used to drive the culture dish to rotate and move, and rotate the culture dish to the culture medium filling mechanism 10; the culture dish rotation motion mechanism 9 includes a turntable 23, and a plurality of limiting holes are opened along the circumference of the top of the turntable 23. The diameter of the limiting holes is smaller than the diameter of the culture dish. A lifting cylinder 24 is provided below the turntable 23, and the piston rod of the lifting cylinder 24 is vertically upward. The end of the piston rod of the lifting cylinder 24 is fixedly connected to the lifting plate 25. The lifting cylinder 24 pushes the lifting plate 25, and the lifting plate 25 pushes the culture dish upward, so that the culture dish is separated from the turntable 23.

[0059] The culture medium filling mechanism 10 is used to perform quantitative culture medium filling processing according to specific requirements of the test.

[0060] The third clean bench 1 is internally equipped with a culture dish rotary tower loading mechanism 21, a culture dish manipulator 8, a culture dish coding module 22, a culture medium filling mechanism 10, a culture dish shaking clamping mechanism 11 and a culture medium covering temporary storage mechanism 12. The culture dish manipulator 8 is located on one side of the culture dish rotary tower loading mechanism 21, the culture dish shaking clamping mechanism 11 is located on one side of the culture dish rotating motion mechanism 9, and the culture medium covering temporary storage mechanism 12 is located on the rear side of the culture dish shaking clamping mechanism 11. The culture dish rotary tower loading mechanism 21, the culture dish manipulator 8, the culture dish coding module 22, the culture medium filling mechanism 10 and the culture medium covering temporary storage mechanism 12 all belong to the existing technology, and the specific structure and implementation principle will not be repeated here.

[0061] The culture dish rotating tower loading mechanism 21 is used to hold empty culture dishes and can rotate 360 ​​degrees;

[0062] The culture dish manipulator 8 is used to grab the culture dishes in the culture dish rotary tower loading mechanism 21;

[0063] The culture dish coding module 22 is used to code the culture dishes grasped by the culture dish manipulator 8;

[0064] The culture medium filling mechanism 10 is used to fill the culture dish with culture medium;

[0065] The culture dish shaking gripper mechanism 11 is used to grab the empty culture dish cover on the culture dish rotating mechanism 9 and shake and mix the culture dish filled with liquid;

[0066] The culture dish shaking clamping mechanism 11 is installed on the top of the turntable 23, including a mounting base, a clamping cylinder A26, a motor 31 and a shaking arm 32. The mounting base includes a column 33 and a support plate 34. The column 33 is fixedly connected to the top of the turntable 23, and the support plate 34 is fixedly connected to the top of the column 33. The motor 31 is vertically installed on the top of the support plate 34. A U-shaped block 35 is installed at the bottom of the support plate 34. The shaking arm 32 is located below the support plate 34. The upper end of the shaking arm 32 is located in the U-shaped block 35. The U-shaped block 35 is slidably connected to the shaking arm 32. The clamping cylinder A26 is located on the support plate 34. Below, the clamping cylinder A26 is installed on the rocking arm 32. A rotating shaft is installed on the top of the clamping cylinder A26. The output shaft of the motor 31 passes downward through the support plate 34. The output shaft of the motor 31 is connected to the rotating shaft through a cam 36. The cam 36 is provided with two through holes, one of which is rotatably connected to the output shaft of the motor 31, and the other is rotatably connected to the rotating shaft. The motor 31 drives the clamping cylinder A26 and the rocking arm 32 to rotate through the cam 36. The rocking arm 32 slides relative to the U-shaped block 35. During the rotation process, the clamping cylinder A26 drives the clamped culture dish to shake. The two clamps A27 of the clamping cylinder A26 are respectively fixedly connected to the arc-shaped clamping plates 28. The two arc-shaped clamping plates 28 are arranged opposite to each other and form a circle. The two clamps A27 of the clamping cylinder A26 are used to drive the two arc-shaped clamping plates 28 to clamp the upper cover of the culture dish.

[0067] When the culture dish cover needs to be removed, the lifting cylinder 24 drives the lifting plate 25 to move upward, and the lifting plate 25 lifts the culture dish so that the height of the culture dish cover matches the height of the arc-shaped clamping plate 28, making it convenient for the arc-shaped clamping plate 28 to clamp and fix the culture dish cover.

[0068] The culture medium covering temporary storage mechanism 12 is used to add culture medium for covering liquid covering treatment;

[0069] The rear ends of the three clean benches 1 are equipped with a culture medium heating module and multiple culture medium holding containers.

[0070] The culture medium heating module is used to heat the culture medium to prevent it from cooling and solidifying;

[0071] Culture medium container, used to hold sterilized culture medium.

[0072] Working principle:

[0073] (1) The sample to be tested is manually loaded onto the sample loading bracket 13. Each sample bottle containing the sample is coded on the surface for automatic identification by scanning and storing relevant information of the test and sample processing.

[0074] (2) Manually load the disposable sterile culture dishes into the culture dish rotating tower loading mechanism 21 in advance to ensure the number of culture dishes in a single batch, and manually put the empty sample bottles into the empty container holder 14. The empty container holder 14 can hold containers such as test tubes, Erlenmeyer flasks, and beakers.

[0075] (3) After the preparation is completed, start the clean bench 1 and perform ultraviolet disinfection on the inside for no less than 30 minutes to ensure the cleanliness of the inside.

[0076] (4) After the clean bench 1 is in operation, the sample manipulator 2 grabs the samples to be tested from the sample loading bracket 13 in order, and moves the samples to the position of the automatic code scanning module 15 for code scanning and identification, which is used to match the relevant information of the samples. The relevant process of the sample processing procedure is automatically carried out according to the relevant information after the code scanning, and the relevant information is saved and recorded throughout the process.

[0077] (5) After the sample code is scanned and identified, the sample manipulator 2 grabs the sample to be tested and places it in the rotary opening mechanism 16 station to perform a rotary opening process on the sample bottle.

[0078] (6) After the lid is opened, the sample manipulator 2 grabs the uncapped sample bottle and moves it to the vibration mixing mechanism 6 to perform vibration mixing on the sample.

[0079] (7) The sample manipulator 2 moves to the empty container holder 14, grabs the empty container and moves it to the weighing sensor module 3, and the weighing sensor module 3 performs automatic zeroing processing.

[0080] (8) The driving device is used to drive the pipetting module 7 to move. The pipetting module 7 moves to the tip loading mechanism 17. After the pipetting module 7 is inserted into a new pipetting tip, it moves to the top of the sample bottle to be pipetted on the vibration mixing mechanism 6.

[0081] (9) The pipetting module 7 uses the pipette tip to slowly enter the sample bottle for precise liquid aspiration, and is moved to the empty sample bottle on the weighing sensor module 3 through the driving device, and the quantitatively aspirated sample is discharged into the empty sample bottle. After completion, the driving device drives the pipetting module 7 to move to the waste collection module 20 to discharge the used pipette tip to prevent secondary contamination.

[0082] (10) After the sample is added to the empty sample bottle on the weighing sensor module 3, the diluent filling mechanism 4 automatically adds diluent to the sample bottle according to the volume of the filled sample, and the weighing sensor module 3 provides real-time feedback to accurately add the diluent.

[0083] (11) After the diluent is added, the sample manipulator 2 grabs the diluted sample bottle and moves it to the metal bath heating module 5. The metal bath heating module 5 performs heating or non-heating according to the detection requirements.

[0084] (12) The automated system can perform automated gradient dilution processing according to the test project requirements. The automated system belongs to the existing technology and will not be described in detail here.

[0085] (13) After the culture dish rotary tower loading mechanism 21 rotates to a fixed position, the culture dish manipulator 8 grabs the empty culture dish and takes it to the culture dish coding module 22 for culture dish coding processing.

[0086] (14) After the culture dish is coded, the culture dish manipulator 8 grabs the coded culture dish and moves it to the culture dish rotating mechanism 9 and carries the empty culture dish to the work station of the culture medium filling mechanism 10, waiting to be filled with samples and culture medium.

[0087] (15) The culture dish shaking clamp mechanism 11 grabs the upper cover of the empty culture dish, and the driving device carries the pipetting module 7 to absorb a quantitative liquid sample from the diluted sample bottle placed on the metal bath heating module 5, and moves it to the empty culture dish at the culture medium adding mechanism station for drainage processing. At the same time, the culture medium filling mechanism 10 performs a quantitative culture medium filling process according to the specific requirements of the test.

[0088] (16) After the diluted sample and culture medium are added, the culture dish shaking clamp mechanism 11 puts the culture dish cover back on the culture dish, grabs the culture dish that has been filled with the two liquids, and shakes and mixes them.

[0089] (17) After the two liquids in the culture dish are mixed, the culture dish manipulator 8 grabs the culture dish and puts it into the culture medium covering temporary storage mechanism 12. After the culture medium and sample mixture cools down, the culture medium is continuously added through the culture medium covering temporary storage mechanism 12 for covering liquid covering treatment.

[0090] (18) After the culture medium is covered, the culture dish manipulator 8 grabs the inoculated culture dish and puts it into the culture dish rotating tower loading mechanism 21, waiting for relevant personnel to take it out.

[0091] (19) The culture medium container is customized to meet the requirements for the use of the culture medium.

[0092] (20) The culture medium container is filled with sterilized culture medium and placed in the culture medium heating module for heating treatment to prevent the culture medium from cooling and solidifying.

[0093] (21) After all samples have been poured and inoculated, the automated system will prompt processing and record all data during the processing so that personnel can view or upload it to the system.

[0094] (22) The settings in the automation system are displayed through the operating screen display setting module to perform settings, queries, uploads and other operations.

[0095] The workstation in the present invention uses an automated operation mode to draw or gradiently dilute the sample that needs to be poured and inoculated from a standard container (gradient dilution definition: a technology that gradually reduces the solute concentration in a solution. Specifically, this technology continuously reduces the solute concentration in the solution to a lower level, usually by taking out a certain volume of solution and adding more solvent. For example, 1 mL is taken out from this solution and 9 mL of diluent is added to obtain a 10% concentration liquid), and transfers the processed sample to an automatically coded culture dish (culture dish definition: a laboratory dish used for microbial or cell culture, consisting of a flat disc-shaped bottom and a cover, generally made of glass or plastic) according to the identified conditions. There is no need for repeated operations, the degree of automation is high, the automatic control is precise, the entire process is automated, the risk of human operation contamination is avoided, and repeated manual labor is reduced, which has a significant effect on pouring and inoculation in microbial testing.

[0096] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention. In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a mechanical connection or an electrical connection, or it can be the internal communication of two elements, it can be a direct connection, or it can be an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances.

[0097] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A laboratory microbial automated pouring inoculation workstation, characterized by: The clean bench comprises the following components installed inside: Sample manipulator, used to grab sample bottles and move them; A weighing sensor module for weighing the sample bottle; A diluent filling mechanism, used for adding diluent into a sample bottle containing a sample; Metal bath heating module, used to heat the sample bottle with diluent added; A vibration mixing mechanism, used for vibrating and mixing the sample in the sample bottle; The pipetting module is used to drive the pipette tip to move and perform quantitative liquid aspiration operations; A culture dish manipulator, used to grab the culture dish and move it; The culture dish rotation mechanism is used to drive the culture dish to rotate; A culture medium filling mechanism is used to quantitatively fill the culture medium into the culture dish; The culture dish shaking gripper mechanism is used to grab the culture dish cover and shake and mix the culture dish filled with culture medium; The culture medium covering temporary storage mechanism is used to add culture medium into the culture dish for covering liquid covering treatment.

2. A laboratory microbial automated pouring inoculation workstation according to claim 1, characterized in that: A sample loading bracket and an empty container bracket are installed inside the clean bench. The sample loading bracket is used to hold sample bottles filled with samples, and the empty container bracket is used to hold empty sample bottles.

3. A laboratory microbial automated pouring inoculation workstation according to claim 1, characterized in that: An automatic code scanning module and a rotary cover opening mechanism are installed inside the clean bench. The automatic code scanning module is used to scan and identify the sample bottle, and the rotary cover opening mechanism is used to rotate and open the sample bottle grasped by the sample manipulator.

4. A laboratory microbial automated pouring inoculation workstation according to claim 1, characterized in that: A gun tip loading mechanism is installed inside the clean workbench, and the gun tip loading mechanism is located below the pipetting module. A pipette gun is installed at the lower end of the pipetting module. The gun tip loading mechanism provides a pipette tip for the pipette gun in the pipetting module. An export plate is installed on the front side of the gun tip loading mechanism. The cross-section of the export plate is L-shaped. The export plate is continuously provided with a number of positioning holes along its length, and a pipette tip is vertically inserted into the positioning hole.

5. A laboratory microbial automated pouring inoculation workstation according to claim 4, characterized in that: A waste collection module is installed inside the clean bench. The waste liquid collection module is located at the front side of the tip loading mechanism. The waste collection module is used to collect the pipette tip after the liquid aspiration is completed.

6. A laboratory microbial automated pouring inoculation workstation according to claim 5, characterized in that: A driving device is installed inside the clean bench, and the driving device drives the pipetting module to move toward the vibration mixing mechanism to perform a quantitative liquid aspiration operation, the driving device drives the pipetting module to move toward the empty sample bottle at the weighing sensor module to perform a liquid discharge operation, and the driving device drives the pipetting module to move toward the waste collection module to perform a pipette tip discharge operation; The driving device includes three linear modules, two of which are arranged in parallel and spaced apart in front and back, and the third linear module is located on top of the two linear modules and fixedly connected to the two sliders of the two linear modules. The slider of the third linear module is fixedly connected to the pipetting module.

7. A laboratory microbial automated pouring inoculation workstation according to claim 1, characterized in that: The clean bench is internally equipped with a culture dish rotary tower loading mechanism and a culture dish coding module. The culture dish rotary tower loading mechanism is used to hold empty culture dishes, and the culture dish coding module is used to code the culture dishes grasped by the culture dish robot.

8. A laboratory microbial automated pouring inoculation workstation according to claim 1, characterized in that: The culture dish rotation mechanism includes a turntable, and a plurality of limiting holes are opened along the circumference of the top of the turntable. The diameter of the limiting holes is smaller than the diameter of the culture dish. A lifting cylinder is provided under the turntable, and the piston rod of the lifting cylinder is vertically upward. The end of the piston rod of the lifting cylinder is fixedly connected to a lifting plate. The lifting cylinder pushes the lifting plate, and the lifting plate pushes the culture dish upward.

9. A laboratory microbial automated pouring inoculation workstation according to claim 8, characterized in that: A culture dish shaking clamp mechanism is installed on the top of the turntable, and the culture dish shaking clamp mechanism includes a mounting seat, a clamping cylinder, a motor and a shaking arm. The motor is vertically installed on the top of the mounting seat, and the shaking arm is slidably connected to the bottom of the mounting seat. The clamping cylinder is installed at the end of the shaking arm, and the output shaft of the motor is rotatably connected to the top of the clamping cylinder through a cam. The two clamps A of the clamping cylinder A are respectively fixedly connected with arc-shaped clamping plates, and the two arc-shaped clamping plates are arranged opposite to each other and form a circle. The two clamps A of the clamping cylinder A are used to drive the two arc-shaped clamping plates to clamp the culture dish cover.

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