Preparation method of detection card for detecting novel coronavirus and detection card
By using gold nanorods to prepare a conjugation pad and a reaction pad with a monoclonal antibody against the novel coronavirus, and assembling them into a colloidal gold immunochromatographic test strip, the problem of low sensitivity in colloidal gold immunochromatography was solved, achieving higher detection sensitivity and stability.
CN118311253BActive Publication Date: 2026-05-29DAAN GENE CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- DAAN GENE CO LTD
- Filing Date
- 2023-01-09
- Publication Date
- 2026-05-29
Smart Images
Figure CN118311253B_ABST
Abstract
The embodiment of the application belongs to the technical field of immunological detection in vitro diagnostic reagents, and relates to a preparation method of a detection card for detecting a novel coronavirus, comprising the following steps: preparing a gold nanorod solution; mixing the gold nanorod solution with a novel coronavirus monoclonal antibody to prepare a gold nanorod-antibody complex solution; preparing a binding pad and a sample pad through the gold nanorod-antibody complex solution; performing antibody coating on a nitrocellulose membrane to obtain a reaction pad coated with antibodies; assembling the binding pad, the sample pad and the coated membrane to obtain a colloidal gold immunochromatography test strip; and assembling the colloidal gold immunochromatography test strip and a shell to obtain the detection card. The application effectively improves the sensitivity of detection.
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