A method for inducing haploids through radiation pollination of Camellia oleifera flowers

By using radiation pollination and embryo rescue methods, the problems of long breeding cycle and low efficiency of Camellia oleifera were solved, and haploid Camellia oleifera plants were successfully cultivated, establishing an efficient breeding system.

CN118318730BActive Publication Date: 2026-06-30CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Application Number
CN202410622419.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-05-20
Publication Date
2026-06-30
Estimated Expiration
2044-05-20

AI Technical Summary

Technical Problem

Existing methods for breeding Camellia oleifera are time-consuming and inefficient, and haploid Camellia oleifera plants have not been successfully cultivated through anther culture.

Method used

The method of combining radiation pollination with embryo rescue involves irradiating pollen before pollination, followed by early isolation, culture and induction of embryos, and obtaining camellia haploids through embryogenic callus and somatic embryogenesis pathways.

Benefits of technology

The successful cultivation of haploid Camellia oleifera plants has shortened the breeding time, improved breeding efficiency, and established an efficient Camellia oleifera breeding system.

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Abstract

This invention discloses a method for inducing haploids in Camellia oleifera through radiation pollen pollination, belonging to the field of plant tissue culture. The method includes the following steps: irradiating Camellia oleifera pollen; determining the viability and germination rate of the irradiated pollen; performing hybridization pollination using the irradiated pollen; selecting the pollinated embryos and inoculating them into a culture medium for embryo rescue; and screening the plants obtained from the embryo rescue for ploidy identification to identify haploids. This invention creates a new approach for cultivating haploids in Camellia oleifera, applicable to both the Camellia oleifera and Camellia brevicornuate groups, and can be applied to the breeding of Camellia oleifera varieties, accelerating the Camellia oleifera breeding process.
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