A passive double-volume droplet synchronous generation and collection chip based on PDMS microchannels, device and method

Abstract

The application discloses the technical field of microfluidics, and relates to a passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel, a device and a method, the chip comprising a chip substrate, an inlet area, a main channel area, an outlet area, a liquid inlet device and a collection device, wherein the inlet area, the main channel area and the outlet area are all arranged in the chip substrate, the input end of the inlet area is connected with the liquid inlet device, the output end of the inlet area is connected with the input end of the main channel area, the output end of the main channel area is connected with the input end of the outlet area, and the output end of the outlet area is connected with the collection device.The passive double-volume droplet synchronous generation and collection chip based on the PDMS microchannel, the device and the method can simply and efficiently realize the generation, separation and collection of double-volume droplets without adding a physical field.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, and in particular to a passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel, a device and a method. BACKGROUND

[0002] Microfluidics refers to the science and technology of systems that handle or manipulate small volumes of fluid (in the nanoliter to microliter range) using microchannels (in the tens to hundreds of micrometers in size), and is regarded as a new research field that intersects with biology, chemistry, medicine, fluidics, electronics, materials, mechanics and other disciplines.

[0003] Droplet microfluidics is a subcategory of microfluidics, and droplet microfluidics technology produces and separates microdroplets through the introduction of immiscible multiphase flow into microchannels. There are currently two main research directions for droplet microfluidics: (1) generating microscale flow reactors for micro total analysis systems (μTAS); and (2) preparing complex droplet particles for material research.

[0004] In order to achieve high-purity preparation of microdroplets, domestic and foreign researchers have proposed a variety of microdroplet fusion methods. Currently, the existing microdroplet fusion methods can be mainly divided into the following categories: (1) by means of an external force field, microdroplets are induced by electric or magnetic force to achieve generation and collection; (2) by designing the shape and size of the flow channel structure in the microfluidic chip, the deformation condition of the mixed phase interface is changed to achieve droplet separation and generation. Although the droplet fusion method using electric or magnetic field is simple, it has a high cost, and when the external force acts on the microdroplet, it may cause certain damage to the biological sample, making it difficult to be widely applied to the needs of biomedical microfluidic technology. The method relying on the change of the shape and size of the flow channel structure is difficult to separate droplets of different volumes, and the application is limited, and the separation efficiency is low. SUMMARY

[0005] The purpose of the present application is to overcome the deficiencies in the prior art, and to provide a passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel, a device and a method, which can achieve high-throughput and separation of double-volume droplets without the addition of a physical field.

[0006] To achieve the above-mentioned purpose, the present application is implemented by using the following technical solutions:

[0007] In a first aspect, the present application provides a passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel, comprising: a chip substrate, an inlet region, a main channel region, an outlet region, a liquid inlet device and a collection device.

[0008] The inlet area, the main channel area and the outlet area are arranged in the chip substrate, the input end of the inlet area is connected with the liquid inlet device, the output end of the inlet area is connected with the input end of the main channel area, the output end of the main channel area (9) is connected with the input end of the outlet area, and the output end of the outlet area is connected with the collection device.

[0009] The inlet area is a cross-shaped flow channel arranged in an axisymmetric manner, and the outlet area is a three-pronged outlet flow channel.

[0010] The continuous phase reagent and the dispersed phase reagent are input into the inlet area through the liquid inlet device, and the passive synchronous double-volume droplets are generated at the intersection of the inlet area, and the generated double-volume droplets are separated at the outlet area and collected through the collection device.

[0011] The cross-sectional height of the flow channel of the inlet area, the main channel area and the outlet area is 5.8-6.2 μm.

[0012] The cross-sectional width of the flow channel of the main channel area is 23-25 μm.

[0013] Further, the chip substrate is formed by soft photolithography of a PDMS material.

[0014] And / or, the separation structure is in a cylindrical shape, and the diameter of the separation structure is 3.8-4.2 μm.

[0015] Further, the inlet area includes a cross-shaped flow channel arranged in an axisymmetric manner, and specifically includes a dispersed phase flow channel, a first continuous phase flow channel, a second continuous phase flow channel and a mixed phase flow channel.

[0016] The liquid inlet device includes a first liquid inlet conduit, a second liquid inlet conduit and a third liquid inlet conduit.

[0017] The input end of the dispersed phase flow channel, the input end of the first continuous phase flow channel and the input end of the second continuous phase flow channel are respectively connected to the first liquid inlet conduit, the third liquid inlet conduit and the second liquid inlet conduit.

[0018] The output end of the mixed phase flow channel is connected with the main channel area.

[0019] The output end of the dispersed phase flow channel, the output end of the first continuous phase flow channel and the output end of the second continuous phase flow channel are connected to the input end of the mixed phase flow channel.

[0020] Further, at least one of the following conditions is met:

[0021] The cross-sectional width of the dispersed phase flow channel and the mixed phase flow channel is 15-17 μm.

[0022] The cross-sectional width of the first continuous phase flow channel and the second continuous phase flow channel is 14-16 μm;

[0023] The first liquid inlet conduit, the second liquid inlet conduit and the third liquid inlet conduit comprise a Teflon hose with an inner diameter of 0.4-0.6 μm.

[0024] Further, the outlet region comprises a non-uniform-width three-pronged outlet flow channel, specifically comprising a first outlet flow channel, a second outlet flow channel and a third outlet flow channel;

[0025] The collection device comprises a first collection conduit, a second collection conduit and a third collection conduit;

[0026] The first outlet flow channel is coaxially arranged with the main channel region;

[0027] The second outlet flow channel and the third outlet flow channel are arranged in axial symmetry with the first outlet flow channel;

[0028] The input end of the first outlet flow channel, the input end of the second outlet flow channel and the input end of the third outlet flow channel are all connected with the main channel region;

[0029] The output end of the first outlet flow channel, the output end of the second outlet flow channel and the output end of the third outlet flow channel are respectively connected with the first collection conduit, the second collection conduit and the third collection conduit.

[0030] Further, at least any one of the following conditions is met:

[0031] The cross-sectional width of the first outlet flow channel is 19-21 μm;

[0032] The cross-sectional width of the second outlet flow channel and the third outlet flow channel is 3.3-3.7 μm;

[0033] The first collection conduit, the second collection conduit and the third collection conduit comprise a Teflon hose with an inner diameter of 0.4-0.6 μm.

[0034] In a second aspect, the present application provides a passive double-volume droplet synchronous generation and collection device based on a PDMS microchannel, comprising the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel.

[0035] Further, the first injection device, the second injection device and the third injection device are further included, which are respectively connected with the first liquid inlet conduit, the second liquid inlet conduit and the third liquid inlet conduit.

[0036] Further, the following are further included:

[0037] The first droplet collection test tube is used for collecting the liquid droplets flowing out of the middle flow channel of the three-pronged outlet flow channel;

[0038] Second droplet collection test tube for collecting the liquid droplets flowing out of the two side flow channels of the three-pronged outlet flow channel.

[0039] In a third aspect, the application provides a passive double-volume droplet synchronous generation and collection method based on a PDMS microchannel, based on the passive double-volume droplet synchronous generation and collection device based on a PDMS microchannel or implementation, the method comprises:

[0040] The first injection device is filled with a dispersed phase reagent, and the second injection device and the third injection device are filled with a continuous phase reagent;

[0041] The passive double-volume droplet synchronous generation and collection device based on a PDMS microchannel is installed;

[0042] The injection flow rates of the first injection device, the second injection device and the third injection device are set respectively, and the first injection device, the second injection device and the third injection device are simultaneously turned on;

[0043] The liquid droplets flowing out of the middle flow channel of the three-pronged outlet flow channel are collected at the first droplet collection test tube, and the liquid droplets flowing out of the two side flow channels of the three-pronged outlet flow channel are collected at the second droplet collection test tube.

[0044] Compared with the prior art, the application has the following beneficial effects:

[0045] The passive double-volume droplet synchronous generation and collection chip, device and method based on a PDMS microchannel provided by the application are provided with a liquid inlet device and a collection device on the chip, an inlet region, a main channel region and an outlet region are opened in the chip, the inlet region is a cross-shaped flow channel, and a separation structure connecting the upper and lower surfaces of the substrate is arranged at the central intersection region of the cross-shaped flow channel, the outlet region is a three-pronged outlet flow channel, and the cross-sectional width and the cross-sectional height of the flow channel are limited, the dispersed phase reagent and the continuous phase reagent intersect at a certain angle at the inlet region, the continuous phase is elongated and finally dispersed into droplets due to the fact that the continuous phase part blocks the dispersed phase at the intersection, and finally collected at the outlet region after flowing through the main channel region, so that the generation, separation and collection of double-volume droplets can be simply and efficiently realized on the basis of not adding a physical field. BRIEF DESCRIPTION OF DRAWINGS

[0046] Figure 1 is a structural schematic diagram of the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel provided by the embodiment of the application;

[0047] Figure 2 is a structural schematic diagram of the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel provided by the embodiment of the application;

[0048] Figure 3 is a top view of the passive double-volume droplet synchronous generation and collection chip based on PDMS microchannels provided by Embodiment 1 of the present application;

[0049] Figure 4 is a front view of the passive double-volume droplet synchronous generation and collection chip based on PDMS microchannels provided by Embodiment 1 of the present application;

[0050] Figure 5 is an experimental result simulation effect diagram of the inlet area and the main channel area of the passive double-volume droplet synchronous generation and collection chip based on PDMS microchannels provided by the present application.

[0051] In the figure: 1-chip substrate; 2-dispersed phase flow channel; 3-first continuous phase flow channel; 4-second continuous phase flow channel; 5-mixed phase flow channel; 6-first liquid inlet conduit; 7-second liquid inlet conduit; 8-third liquid inlet conduit; 9-main channel area; 10-separation structure; 11-first outlet flow channel; 12-second outlet flow channel; 13-third outlet flow channel; 14-first collection conduit; 15-second collection conduit; 16-third collection conduit. DETAILED DESCRIPTION

[0052] The present application will be further described below in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solutions of the present application, and cannot be used to limit the protection scope of the present application.

[0053] Embodiment 1

[0054] The present embodiment provides a passive double-volume droplet synchronous generation and collection chip based on PDMS microchannels, as shown in Figure 1 The chip comprises:

[0055] a chip substrate 1, an inlet area, a main channel area 9, an outlet area, a liquid inlet device and a collection device;

[0056] The inlet area, the main channel area 9 and the outlet area are all provided on the chip substrate 1, the input end of the inlet area is connected with the liquid inlet device, the output end of the inlet area is connected with the input end of the main channel area 9, the output end of the main channel area 9 is connected with the input end of the outlet area, and the output end of the outlet area is connected with the collection device;

[0057] As shown in Figure 2 The inlet area is a cross-shaped flow channel arranged in axial symmetry, the outlet area is a trident-shaped outlet flow channel, and the central intersection area of the cross-shaped flow channel is provided with a separation structure 10 connecting the upper and lower surfaces of the substrate;

[0058] The continuous phase reagent and the dispersed phase reagent are input into the inlet area through the liquid inlet device, and the double-volume droplets are passively and synchronously generated at the intersection of the inlet area, and the generated double-volume droplets are separated at the outlet area and collected through the collection device.

[0059] The cross-sectional height of the flow channel of the inlet area, the main channel area 9 and the outlet area is 6 μm; the cross-sectional width of the flow channel of the main channel area 9 is 24 μm.

[0060] In this embodiment, the chip substrate 1 is soft photolithographed by a PDMS material.

[0061] The separation structure 10 is cylindrical, and the diameter of the separation structure 10 is 4 μm.

[0062] As shown in Figure 2 The inlet area is arranged as an axisymmetric cross-shaped flow channel, which specifically includes a dispersed phase flow channel 2, a first continuous phase flow channel 3, a second continuous phase flow channel 4 and a mixed phase flow channel 5.

[0063] The liquid inlet device includes a first liquid inlet conduit 6, a second liquid inlet conduit 7 and a third liquid inlet conduit 8.

[0064] The input end of the dispersed phase flow channel 2, the input end of the first continuous phase flow channel 3 and the input end of the second continuous phase flow channel 4 are respectively connected to the first liquid inlet conduit 6, the second liquid inlet conduit 7 and the third liquid inlet conduit 8.

[0065] The output end of the mixed phase flow channel 5 is connected to the main channel area 9; the output end of the dispersed phase flow channel 2, the output end of the first continuous phase flow channel 3 and the output end of the second continuous phase flow channel 4 are connected to the input end of the mixed phase flow channel 5, forming an intersection area of the cross-shaped flow channel.

[0066] The cross-sectional width of the dispersed phase flow channel 2 and the mixed phase flow channel 5 is 16 μm.

[0067] The cross-sectional width of the first continuous phase flow channel 3 and the second continuous phase flow channel 4 is 15 μm.

[0068] The intersection area is provided with a cylindrical separation structure 10 connecting the upper and lower surfaces of the substrate, the diameter of the cylindrical separation structure 10 is 4 μm, and the cross-sectional height is the same as the flow channel cross-sectional height, which is 6 μm.

[0069] The first liquid inlet conduit 6, the second liquid inlet conduit 7 and the third liquid inlet conduit 8 are all Teflon hoses with an inner diameter of 0.5 μm.

[0070] The outlet area is arranged as a three-pronged outlet flow channel with unequal widths, which specifically includes a first outlet flow channel 11, a second outlet flow channel 12 and a third outlet flow channel 13.

[0071] The collecting device comprises a first collecting conduit 14, a second collecting conduit 15, and a third collecting conduit 16.

[0072] The first outlet flow channel 11 is coaxially arranged with the main channel area 9; the second outlet flow channel 12 and the third outlet flow channel 13 are symmetrically arranged with the first outlet flow channel 11 as the axis; the input ends of the first outlet flow channel 11, the second outlet flow channel 12, and the third outlet flow channel 13 are connected with the main channel area 9; and the output ends of the first outlet flow channel 11, the second outlet flow channel 12, and the third outlet flow channel 13 are respectively connected with the first collecting conduit 14, the second collecting conduit 15, and the third collecting conduit 16.

[0073] The cross-sectional width of the first outlet flow channel 11 is 20 μm; and the cross-sectional widths of the second outlet flow channel 12 and the third outlet flow channel 13 are 3.5 μm.

[0074] The first collecting conduit 14, the second collecting conduit 15, and the third collecting conduit 16 comprise Teflon hoses with an inner diameter of 0.5 μm.

[0075] Figure 3 is a top view of the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel provided in Embodiment 1 of the present application, Figure 4 is a front view of the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel provided in Embodiment 1 of the present application.

[0076] The working principle of the passive double-volume droplet synchronous generation and collection chip based on a PDMS microchannel provided in the present embodiment is as follows:

[0077] In the cross intersection flow channel, the dispersed phase reagent and the continuous phase reagent flow through the positive channel and meet at the intersection at a certain angle. At this time, since the continuous phase partially blocks the dispersed phase at the intersection, a shear gradient is generated at the two-phase mixing interface, the continuous phase is elongated and finally broken into droplets. The size of the droplets is closely related to the size of the shear stress, and the size of the shear stress changes with the flow rate, the fluid viscosity, and the aspect ratio of the channel cross section.

[0078] The flow rates of the dispersed phase reagent and the continuous phase reagent are controlled to enter the device at certain flow rates, the intersection of the cross-flow channel is provided with a cylindrical separation structure, and the width of the mutation section of the connection between the primary channel and the secondary channel is set, so that the flow pattern of the dispersed phase reagent is changed, the shear stress received by the dispersed phase reagent is increased, the dispersed phase is dispersed into super-large volume droplets in the mixed phase flow channel, and is dispersed again at the connection surface of the primary channel and the secondary channel, and is unevenly broken into large volume droplets and small volume droplets. The large volume droplets are generated at the center of the flow channel and flow along the axis to the first collection flow channel, and finally are collected at the outlet of the first collection conduit; the small volume droplets are extruded to the wall of the primary channel after being generated, flow along the wall of the primary channel to the second collection flow channel and the third collection flow channel, and finally are collected at the outlet of the second collection conduit and the outlet of the third collection conduit.

[0079] Figure 5 The experimental result simulation effect diagram of the passive double-volume droplet synchronous generation and collection chip based on the PDMS microchannel provided in the embodiment is shown in the drawings, wherein the blue part is the continuous phase reagent, and the red part is the dispersed phase reagent, as shown in Figure 5 The experimental result simulation effect diagram of the passive double-volume droplet synchronous generation and collection chip based on the PDMS microchannel provided in the embodiment is shown in the drawings, wherein the blue part is the continuous phase reagent, and the red part is the dispersed phase reagent, as shown in

[0080] Embodiment 2

[0081] The embodiment provides a passive double-volume droplet synchronous generation and collection device based on a PDMS microchannel, which comprises the passive double-volume droplet synchronous generation and collection chip based on the PDMS microchannel provided in the embodiment 1.

[0082] In the embodiment, the passive double-volume droplet synchronous generation and collection device based on the PDMS microchannel further comprises an injection device, a second injection device and a third injection device, which are connected with the first liquid inlet conduit, the second liquid inlet conduit and the third liquid inlet conduit respectively.

[0083] In the embodiment, the passive double-volume droplet synchronous generation and collection device based on the PDMS microchannel further comprises a large volume droplet collection test tube and a small volume droplet collection test tube, wherein the large volume droplet collection test tube is used for collecting large volume droplets, and the small volume droplet collection test tube is used for collecting small volume droplets.

[0084] Embodiment 3

[0085] The embodiment provides a passive double-volume droplet synchronous generation and collection method based on a PDMS microchannel, which is realized by using the passive double-volume droplet synchronous generation and collection device based on the PDMS microchannel provided in the embodiment 2, and comprises the following steps:

[0086] The first injection device is filled with a non-pure water solution with a viscosity of 2E-3 Pa.s, and the second and third injection devices are filled with a blended oil with a viscosity of 16.9E-3 Pa.s;

[0087] The first liquid inlet conduit 6 is connected to the first injection device, the second liquid inlet conduit 7 is connected to the second injection device, and the third liquid inlet conduit 8 is connected to the third injection device;

[0088] The flow rate of the injection devices is set: the flow rate of the first injection device is 2ul / min, and the flow rates of the second and third injection devices are equal, being 4ul / min;

[0089] The first collection conduit 14 is connected to a large-volume droplet collection test tube, and is directly connected to the atmospheric pressure;

[0090] The second collection conduit 15 and the third collection conduit 16 are connected to a small-volume droplet collection test tube, and are directly connected to the atmospheric pressure;

[0091] The first injection device, the second injection device and the third injection device are simultaneously opened, and the two liquid phases are combined at the intersection of the cross-flow channel, and the dispersed phase reagent is dispersed under the blocking action of the cylindrical separation structure, and the continuous phase reagent rapidly fills the empty area, forming an ultra-dispersed phase droplet;

[0092] At the connection between the secondary channel area and the main channel area, the flow state is changed due to the sudden change in the cross section, the shear stress of the dispersed phase reagent is increased, the droplet is dispersed again, and uneven rupture occurs, and two volumes of droplets are stably generated and collected.

[0093] In the description of the present application, it should be understood that the orientations or positional relationships indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like are based on the orientations or positional relationships shown in the drawings, and are used only to explain the relative positional relationships, movement conditions and the like between the components in a certain specific posture, and if the specific posture changes, the directional indications also change accordingly. It is only for the convenience of describing the present application and simplifying the description, and is not intended to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second" and the like are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first", "second" and the like can explicitly or implicitly include one or more of the features. In the description of the present application, unless otherwise stated, the meaning of "a plurality of" is two or more.

[0094] In the description of the present application, it should be noted that unless specifically stated and limited otherwise, the terms "mounting", "connected", "connecting" should be construed broadlyly, for example, can be fixedly connected, can be detachably connected, or integrally connected; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, or can be connected inside two elements. For those skilled in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0095] The embodiments of the present application are described above in combination with the drawings, but the present application is not limited to the above specific embodiments, and the above specific embodiments are only illustrative, but not restrictive. Those skilled in the art can make many forms under the inspiration of the present application without departing from the purpose of the present application and the scope protected by the claims, and these all belong to the protection of the present application.

Claims

1. A PDMS microchannel-based passive two-volume droplet synchronous generation and collection chip, characterized in that, The chip base (1), an inlet area, a main channel area (9), an outlet area, a liquid inlet device and a collection device are included. The inlet area, the main channel area (9) and the outlet area are all arranged in the chip base (1), the input end of the inlet area is connected with the liquid inlet device, the output end of the inlet area is connected with the input end of the main channel area (9), the output end of the main channel area (9) is connected with the input end of the outlet area, and the output end of the outlet area is connected with the collection device. The inlet area is a cross intersection flow channel, the outlet area is a trifurcate outlet flow channel, and the central intersection area of the cross intersection flow channel is provided with a separation structure (10) connecting the upper and lower surfaces of the substrate. The continuous phase reagent and the dispersed phase reagent are input into the inlet area through the liquid inlet device, and the passive synchronous double-volume droplets are generated at the intersection of the inlet area, and the generated double-volume droplets are separated at the outlet area and collected through the collection device. The cross-sectional height of the flow channel of the inlet area, the main channel area (9) and the outlet area is 5.8-6.2μm. The cross-sectional width of the flow channel of the main channel area (9) is 23-25μm. The chip base (1) is made of PDMS material by soft lithography.

2. The PDMS microchannel-based passive double-volume droplet synchronous generation and collection chip according to claim 1, characterized in that: The separation structure (10) is cylindrical, and the diameter of the separation structure (10) is 3.8-4.2μm. The cross intersection flow channel specifically includes a dispersed phase flow channel (2), a first continuous phase flow channel (3), a second continuous phase flow channel (4) and a mixed phase flow channel (5).

3. The PDMS microchannel-based passive double-volume droplet synchronous generation and collection chip according to claim 1, characterized in that: The liquid inlet device includes a first liquid inlet conduit (6), a second liquid inlet conduit (7) and a third liquid inlet conduit (8). The input end of the dispersed phase flow channel (2), the input end of the first continuous phase flow channel (3) and the input end of the second continuous phase flow channel (4) are respectively connected to the first liquid inlet conduit (6), the third liquid inlet conduit (8) and the second liquid inlet conduit (7). The output end of the mixed phase flow channel (5) is connected with the main channel area (9). The output end of the dispersed phase flow channel (2), the output end of the first continuous phase flow channel (3) and the output end of the second continuous phase flow channel (4) are connected with the input end of the mixed phase flow channel (5). At least one of the following conditions is met:

4. The PDMS microchannel-based passive double-volume droplet synchronous generation and collection chip according to claim 3, characterized in that, The cross-sectional width of the dispersed phase flow channel (2) and the mixed phase flow channel (5) is 15-17μm. The cross-sectional width of the first continuous phase flow channel (3) and the second continuous phase flow channel (4) is 14-16μm. The first liquid inlet conduit (6), the second liquid inlet conduit (7) and the third liquid inlet conduit (8) include a Teflon hose with an inner diameter of 0.4-0.6μm. The trifurcate outlet flow channel specifically includes a first outlet flow channel (11), a second outlet flow channel (12) and a third outlet flow channel (13).

5. The PDMS microchannel-based passive double-volume droplet synchronous generation and collection chip according to claim 1, characterized in that: The collection device includes a first collection conduit (14), a second collection conduit (15) and a third collection conduit (16). The first outlet flow channel (11) is coaxially arranged with the main channel area (9), and the second outlet flow channel (12) and the third outlet flow channel (13) are symmetrically arranged with the first outlet flow channel (11) as the axis. ​ The input end of the first outlet flow channel (11), the input end of the second outlet flow channel (12) and the input end of the third outlet flow channel (13) are connected with the main channel area (9); The output end of the first outlet flow channel (11), the output end of the second outlet flow channel (12) and the output end of the third outlet flow channel (13) are connected with the first collection conduit (14), the second collection conduit (15) and the third collection conduit (16) respectively.

6. The PDMS microchannel-based passive double-volume droplet synchronous generation and collection chip according to claim 5, wherein, At least any one of the following is met: The cross-sectional width of the first outlet flow channel (11) is 19-21 μm; The cross-sectional width of the second outlet flow channel (12) and the third outlet flow channel (13) is 3.3-3.7 μm; The first collection conduit (14), the second collection conduit (15) and the third collection conduit (16) comprise a Teflon hose with an inner diameter of 0.4-0.6 μm.

7. A passive two-volume droplet synchronous generation and collection device based on PDMS microchannels, characterized in that, A passive double-volume droplet synchronous generation and collection device based on PDMS microchannels according to any one of claims 1-6.

8. The PDMS microchannel-based passive two-volume droplet synchronous generation and collection device of claim 7, wherein, Further comprising a first injection device, a second injection device and a third injection device connected with the first liquid inlet conduit (6), the second liquid inlet conduit (7) and the third liquid inlet conduit (8) respectively.

9. The PDMS microchannel based passive two-volume droplet synchronous generation and collection device of claim 7, wherein, Further comprising: A first droplet collection test tube for collecting the droplets from the middle flow channel of the trifurcated outlet flow channel; A second droplet collection test tube for collecting the droplets from the two side flow channels of the trifurcated outlet flow channel.

10. A method for passive two-volume droplet synchronous generation and collection based on PDMS microchannels, characterized in that, A passive double-volume droplet synchronous generation and collection device or implementation based on PDMS microchannels according to any one of claims 7 and 8, the method comprising: Filling the first injection device with the dispersed phase reagent and filling the second injection device and the third injection device with the continuous phase reagent; Installing the passive double-volume droplet synchronous generation and collection device based on PDMS microchannels; Setting the injection flow rate of the first injection device, the second injection device and the third injection device respectively and simultaneously opening the first injection device, the second injection device and the third injection device; Collecting the droplets from the middle flow channel of the trifurcated outlet flow channel in the first droplet collection test tube and collecting the droplets from the two side flow channels of the trifurcated outlet flow channel in the second droplet collection test tube.

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