Positive pressure filtration method and apparatus

Abstract

The disclosure provides a positive pressure filtration method and device, the positive pressure filtration method comprises the following steps: acquiring a pressure value range of different liquids in a peptide segment desalting process; setting a plurality of filtration pressures according to the pressure value range; setting a plurality of filtration times according to the plurality of filtration pressures; and switching the filtration pressure in the filtration column according to the filtration time to perform gradient filtration on a solution. The positive pressure filtration method can better solve the problem of inconsistent flow rates of the liquid passing through the filtration plate by splitting the original fixed pressure value and fixed time into a plurality of stages composed of different pressure values and pressure times and assisting with different cycle numbers.

Description

Technical Field

[0001] This disclosure relates to the field of filtration technology, and in particular to a positive pressure filtration method and apparatus. Background Technology

[0002] Positive pressure filtration systems are commonly used in small pharmaceutical plants, food and beverage businesses, factories, mines, hospitals, and laboratories for liquid filtration, clarification, sterilization, and biological purification. The principle of a positive pressure filtration system is to place the filtration module within a high-pressure positive pressure chamber to complete the filtration operation, thereby improving filtration efficiency.

[0003] Traditional positive pressure modules maintain a fixed pressure and time to allow the peptide solution after enzymatic hydrolysis to flow through a portion of the filter columns / membranes, thus filtering out impurities. However, a fixed pressure and time make it difficult to ensure that the flow rate of the peptide solution in all filter columns is the same or similar. This can lead to incomplete removal of impurities from the peptide solution and uneven eluent volume during elution. Furthermore, due to the uneven liquid discharge rate, some filter columns may have completed the flow while many others remain unflowed. In these unflowed columns, air can escape into the already flowed columns, preventing further liquid flow in the remaining columns. This phenomenon also contributes to uneven final results. Summary of the Invention

[0004] To solve the above problems, it is necessary to provide a positive pressure filtration method and device. By breaking down the original fixed pressure value and fixed time into multiple pressure gradients composed of different pressure values ​​and pressure times, and supplementing them with different number of cycles, the problem of inconsistent flow rate of liquid when passing through the filter plate can be solved better.

[0005] According to one aspect of this application, a positive pressure filtration method is provided, comprising:

[0006] Obtain the pressure range of different liquids during peptide desalination;

[0007] Multiple filtration pressures are set according to the pressure range;

[0008] Multiple corresponding filtration times are set according to the multiple filtration pressures;

[0009] The solution is subjected to gradient filtration by switching the filtration pressure in the filtration column according to the filtration time.

[0010] In some embodiments, setting multiple filtration pressures according to the pressure value range includes: setting at least a first filtration pressure and a second filtration pressure, wherein the first filtration pressure corresponds to a first filtration time and the second filtration pressure corresponds to a second filtration time, and wherein the first filtration pressure is greater than the second filtration pressure.

[0011] In some embodiments, setting multiple filtration pressures according to the pressure value range further includes: setting a third filtration pressure, the third filtration pressure corresponding to a third filtration time; when the first filtration pressure and the second filtration pressure are applied, if liquid remains in part of the filter column, the third filtration pressure, the first filtration pressure, and the second filtration pressure are switched sequentially for the next round of filtration; wherein the third filtration pressure is not less than the second filtration pressure.

[0012] In some embodiments, obtaining the pressure range of different liquids during the peptide desalination process includes: testing the pressure range of different media solutions in filter columns of different materials.

[0013] In some embodiments, the first filtration time t1 satisfies: 0.1s≤t1≤0.3s; the second filtration time t2 satisfies: 5s≤t2≤60s; and the third filtration time t3 satisfies: 0.1s≤t2≤10s.

[0014] In some embodiments, performing gradient filtration of the solution by switching the filtration pressure in the filter column according to the filtration time includes: cyclically switching the filtration pressure in the filter column according to the filtration time to perform gradient filtration of the solution.

[0015] In some embodiments, performing gradient filtration of the solution by cyclically switching the filtration pressure in the filter column according to the filtration time includes: the number of cycles being at least two.

[0016] According to another aspect of this application, a positive pressure filtration device is provided, comprising:

[0017] A positive pressure module is used to regulate and maintain the pressure within the filter column, allowing the solution to flow through the filter column and thus filter impurities from the solution; wherein the positive pressure module performs positive pressure filtration through the following method:

[0018] Obtain the pressure range of different liquids during peptide desalination;

[0019] Multiple filtration pressures are set according to the pressure range;

[0020] Multiple corresponding filtration times are set according to the multiple filtration pressures;

[0021] The solution is subjected to gradient filtration by switching the filtration pressure in the filtration column according to the filtration time.

[0022] A support assembly is disposed below the positive pressure module and is used to place the filter column;

[0023] A drive assembly, connected to the positive pressure module, is used to drive the positive pressure module to move toward or away from the filter column.

[0024] In some embodiments, the positive pressure module includes an upper positive pressure chamber and a lower positive pressure chamber. The upper positive pressure chamber is provided with an air inlet, and the bottom of the lower positive pressure chamber is provided with a positive pressure hole. A sealing gasket is provided on the bottom end face of the lower positive pressure chamber, and a through hole corresponding to the positive pressure hole is provided on the sealing gasket.

[0025] In some embodiments, the drive assembly includes a pressure plate and drive components connected to both ends of the pressure plate, the upper positive pressure chamber is fixed to the pressure plate, and the drive components include a lead screw and a motor. Attached Figure Description

[0026] These and / or other aspects and advantages of this application will become clearer and more readily understood from the following detailed description of embodiments of this application taken in conjunction with the accompanying drawings, wherein:

[0027] Figure 1 This is a schematic diagram of the positive pressure filtration method disclosed in this paper;

[0028] Figure 2 This is another schematic diagram of the positive pressure filtration method disclosed herein;

[0029] Figure 3 This is a schematic diagram of the positive pressure filtration device disclosed herein;

[0030] Figure 4 This is a schematic diagram of the positive pressure module disclosed in this publication. Detailed Implementation

[0031] To enable those skilled in the art to better understand this application, the application will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] First, a brief overview of the basic background and main ideas of the positive pressure filtration method and technology used in the peptide desalination process in this application will be provided.

[0033] As mentioned earlier, traditional positive pressure modules maintain a fixed pressure and time to allow the peptide solution after proteolytic hydrolysis to flow through a portion of the filter columns / membranes, thus filtering out impurities. However, a fixed pressure and time make it difficult to ensure that the flow rate of the peptide solution in all filter columns is the same or similar, leading to incomplete removal of impurities and uneven eluent volume. Furthermore, due to the uneven liquid discharge rate, some filter columns may have completed the flow while many others remain unflowed. Air can then escape into the already flowed columns, preventing further liquid flow in the remaining columns, also contributing to uneven results. Therefore, a positive pressure filtration method for peptide solutions is urgently needed to effectively improve the inconsistent flow rate of the peptide solution through the filter columns.

[0034] The application of the positive pressure filtration method provided in this disclosure in peptide solution filtration is described below.

[0035] By breaking down the previously fixed pressure and time into multiple pressure gradients composed of different pressure values ​​and pressure-times, and supplementing them with different number of cycles, the inconsistent flow rate of liquid passing through the filter column can be effectively improved. Taking methanol as an example (methanol is commonly used to activate the packing material in filter columns and is often used as the first step in peptide desalination filtration), a set of optimal pressure-time values ​​was determined (the filter column tested was...). WWP2417-0101R): First filtration pressure is 0.05MPa, first filtration time is 0.1s; second filtration pressure is 0.015MPa, second filtration time is 10s; third filtration pressure is 0.02MPa, third filtration time is 5s; number of cycles is 2.

[0036] Meanwhile, the liquids mainly involved in the peptide desalting process were tested, and the filter column used in the test was... The specific experimental parameters for WWP2417-0101R are as follows:

[0037] Liquid name: methanol; first filtration pressure: 0.05 MPa; first filtration time: 0.1 s; second filtration pressure: 0.015 MPa; second filtration time: 10 s; third filtration pressure: 0.02 MPa; third filtration time: 5 s; number of cycles: 2.

[0038] Liquid name: 0.1% TFA solution; first filtration pressure: 0.1 MPa; first filtration time: 0.1 s; second filtration pressure: 0.04 MPa; second filtration time: 20 s; third filtration pressure: 0.1 MPa; third filtration time: 0.1 s; number of cycles: 2.

[0039] Liquid name: 40% acetonitrile solution; first filtration pressure: 0.05 MPa; first filtration time: 0.1 s; second filtration pressure: 0.035 MPa; second filtration time: 30 s; third filtration pressure: 0.05 MPa; third filtration time: 0.1 s; number of cycles: 3.

[0040] Liquid name: Acidified peptide solution; First filtration pressure: 0.06 MPa; First filtration time: 0.1 s; Second filtration pressure: 0.04 MPa; Second filtration time: 30 s; Third filtration pressure: 0.06 MPa; Third filtration time: 0.1 s; Number of cycles: 3.

[0041] In addition to the above-mentioned tests on the liquids mainly involved in the peptide desalination process under multiple pressure stages, tests were also conducted on filter columns / plates of different materials (Oasis MCX 96-well μElution Plate, Thermo Scientific...). TM SOLAμ TM SPE Plate Tests were conducted on SPE Columns and Plates to determine the preferred pressure ranges for four liquids in the positive pressure filtration method of this disclosure embodiment. The specific experimental parameters are as follows:

[0042] Liquid name: Methanol; First filtration pressure range: 0.02MPa~0.065MPa; First filtration time range: 0.1s~0.25s; Second filtration pressure range: 0.008MPa~0.03MPa; Second filtration time range: 5s~20s; Third filtration pressure range: 0.01MPa~0.025MPa; Third filtration time range: 3s~10s; Number of cycles: 2~4.

[0043] Liquid name: 0.1% TFA solution; first filtration pressure range: 0.08MPa~0.12MPa; first filtration time range: 0.1s~0.2s; second filtration pressure range: 0.02MPa~0.065MPa; second filtration time range: 8s~25s; third filtration pressure range: 0.05MPa~0.12MPa; third filtration time range: 0.1s~0.5s; number of cycles: 2~4.

[0044] Liquid name: 40% acetonitrile solution; first filtration pressure range: 0.03MPa to 0.45MPa; first filtration time range: 0.1s to 0.2s; second filtration pressure range: 0.03MPa to 0.045MPa; second filtration time range: 18s to 38s; third filtration pressure range: 0.03MPa to 0.05MPa; third filtration time range: 0.1s to 1s; number of cycles: 1 to 5.

[0045] Liquid name: Acidified peptide solution; First filtration pressure range: 0.03MPa~0.10MPa; First filtration time range: 0.1s~0.15s; Second filtration pressure range: 0.035MPa~0.08MPa; Second filtration time range: 20s~60s; Third filtration pressure range: 0.05MPa~0.08MPa; Third filtration time range: 0.1s~1s; Number of cycles: 3~6.

[0046] Therefore, in order to verify the consistency of flow rate of different peptide liquids under segmented multiple pressures, especially the consistency of flow rate of different peptide liquids under segmented multiple pressures in different filter columns / plates, the objectivity of the positive pressure filtration method disclosed in this paper is improved.

[0047] Example 1

[0048] Figure 1 A flowchart of a positive pressure filtration method according to an embodiment of the present disclosure is shown.

[0049] like Figure 1 As shown, in step S101, the pressure range of different liquids during the peptide desalting process is obtained; the pressure range of different liquids during the peptide desalting process can be obtained through experimental testing. For example:

[0050] Liquid name: Methanol; First filtration pressure range: 0.02MPa~0.065MPa; First filtration time range: 0.1s~0.25s; Second filtration pressure range: 0.008MPa~0.03MPa; Second filtration time range: 5s~20s; Third filtration pressure range: 0.01MPa~0.025MPa; Third filtration time range: 3s~10s.

[0051] Liquid name: 0.1% TFA solution; first filtration pressure range: 0.08MPa~0.12MPa; first filtration time range: 0.1s~0.2s; second filtration pressure range: 0.02MPa~0.065MPa; second filtration time range: 8s~25s; third filtration pressure range: 0.05MPa~0.12MPa; third filtration time range: 0.1s~0.5s.

[0052] Liquid name: 40% acetonitrile solution; first filtration pressure range: 0.03MPa to 0.45MPa; first filtration time range: 0.1s to 0.2s; second filtration pressure range: 0.03MPa to 0.045MPa; second filtration time range: 18s to 38s; third filtration pressure range: 0.02MPa to 0.05MPa; third filtration time range: 0.1s to 1s.

[0053] Liquid name: Acidified peptide solution; First filtration pressure range: 0.03MPa~0.10MPa; First filtration time range: 0.1s~0.15s; Second filtration pressure range: 0.035MPa~0.08MPa; Second filtration time range: 20s~60s; Third filtration pressure range: 0.05MPa~0.08MPa; Third filtration time range: 0.1s~1s.

[0054] In step S102, multiple filtration pressures are set according to the pressure range, including at least a first filtration pressure and a second filtration pressure. The first filtration pressure corresponds to a first filtration time, and the second filtration pressure corresponds to a second filtration time. The first filtration pressure is greater than the second filtration pressure. The original fixed pressure value and fixed time are divided into multiple pressure gradients composed of different pressure values ​​and pressure times. This solves problems such as suspension and beads at the end of the filter column, which is beneficial to the uniform flow rate of liquid in the filter column and improves filtration efficiency.

[0055] Preferably, setting multiple filtration pressures according to the pressure range further includes setting a third filtration pressure, which corresponds to a third filtration time. After the first and second filtration pressures are applied, if liquid remains in part of the filter column, the third filtration pressure, the first filtration pressure, and the second filtration pressure are switched sequentially for the next round of filtration. The third filtration pressure is not less than the second filtration pressure. The third filtration pressure provides initial power and has a short duration. For the same liquid, after the third filtration pressure has finished filtering, the first and second filtration pressures are switched sequentially, and the cycle continues until all the liquid in the filter column is filtered. This effectively solves the problem that even when some filter columns are depressurized, the remaining filter columns can still filter normally, reducing the loss of experimental samples.

[0056] In this embodiment, the first filtration pressure is greater than the second filtration pressure, and the third filtration pressure is greater than the second filtration pressure. The first filtration pressure and the second filtration pressure form a pressure gradient. The first filtration pressure lasts for a short period of time, which is equivalent to providing the initial filtration power. If the first filtration pressure is maintained, due to errors caused by the manufacturing process of the filter column, it is easy for some liquid in the filter column to flow through first, and the remaining filter column cannot filter normally. Therefore, the filtration pressure is split into the first filtration pressure and the second filtration pressure. According to the experimental results, the second filtration time is the longest, and the second filtration pressure is the main filtration. Moreover, the first filtration pressure and the second filtration pressure form a gradient filtration. When there is less liquid in the filter column, the liquid can be filtered under the action of the first filtration pressure. However, liquid cantilever or bead phenomenon may occur at the end of the filter column. At this time, the second filtration pressure lasts for a longer period of time, so that the liquid in the filter column flows through and reduces sample loss. If some liquid in the filter column still remains after some liquid has flowed through, the third filtration pressure is switched instantly, and a larger pressure value is input into the filter column to make the liquid in the filter column flow. Then, the first filtration pressure and the second filtration pressure are switched in turn until the liquid in the filter column is filtered.

[0057] The first filtration time t1 satisfies: 0.1s ≤ t1 ≤ 0.3s; the second filtration time t2 satisfies: 5s ≤ t2 ≤ 60s; and the third filtration time t3 satisfies: 0.1s ≤ t2 ≤ 10s. For example:

[0058] Liquid name: Methanol, cycle count: 2-4 times. Liquid name: Cycle count: 2-4 times. Liquid name: Cycle count: 1-5 times. Liquid name: Cycle count: 3-6 times.

[0059] Example 2

[0060] Figure 2 Another flowchart of the positive pressure filtration method according to an embodiment of the present disclosure is shown.

[0061] like Figure 2 As shown, the difference between this embodiment and Embodiment 1 is that the filtration pressure in the filter column is cyclically switched according to the filtration time to perform gradient filtration of the solution. Specifically, if there is a large amount of liquid in the filter column and one filtration pressure cycle is not enough to filter it completely, the first filtration pressure and the second filtration pressure can be cyclically switched until the liquid is filtered completely.

[0062] If liquid remains in some filter columns after the first and second filtration pressure cycles are completed, the third, first, and second filtration pressures are switched sequentially for filtration until all liquid in the filter columns is filtered out.

[0063] In this embodiment, the number of cycles is at least two.

[0064] Example 3

[0065] Figure 3 and Figure 4 A structural diagram of a positive pressure filtration device to which the positive pressure filtration method of embodiments of the present disclosure can be applied is shown. The following will be combined with... Figure 3 and Figure 4 Describe the positive pressure filtration device and method.

[0066] The positive pressure filtration device provided in this embodiment includes a positive pressure module 10, a support assembly 20, and a drive assembly 30. In specific applications, the positive pressure module 10 is used to seal the filter column 40 on the support assembly 20 and change the pressure inside the filter column. The drive assembly is connected to the positive pressure module and drives the positive pressure module to move.

[0067] Specifically, such as Figure 3 As shown, the positive pressure module 10 is used to adjust and maintain the pressure inside the filter column so that the solution flows through the filter column to filter impurities in the solution; the support assembly 20 is disposed below the positive pressure module and is used to place the filter column 40; the drive assembly 30 is connected to the positive pressure module 10 and is used to drive the positive pressure module 10 to move toward or away from the filter column; the drive assembly 30 includes a pressure plate 31 and drive components connected to both ends of the pressure plate; the upper positive pressure chamber is fixed on the pressure plate; the drive components include a lead screw and a motor.

[0068] like Figure 4 As shown, the positive pressure module 10 includes an upper positive pressure chamber 11 and a lower positive pressure chamber 12. The upper positive pressure chamber 11 is provided with an air inlet end 111, and the lower positive pressure chamber 12 is provided with a positive pressure hole 121 at the bottom. A sealing gasket 13 is provided on the bottom end face of the lower positive pressure chamber 12, and a through hole corresponding to the positive pressure hole 121 is provided on the sealing gasket 13.

[0069] Preferably, a sound-absorbing plate 14 is provided between the upper positive pressure chamber 11 and the lower positive pressure chamber 12. The sound-absorbing plate 14 has sound-absorbing cotton in its sound-absorbing holes, which buffers the gas entering the positive pressure chamber from the air inlet 111 and helps to improve the stability of the airflow.

[0070] The basic principles of this application have been described above with reference to specific embodiments. However, it should be noted that the advantages, benefits, and effects mentioned in this application are merely examples and not limitations, and should not be considered as essential features of each embodiment of this application. Furthermore, the specific details disclosed above are for illustrative and facilitative purposes only, and are not limitations. These details do not limit the application to the necessity of employing the aforementioned specific details. Additionally, features from one embodiment can be combined with features from one or more other embodiments to obtain more embodiments.

[0071] The block diagrams of devices, apparatuses, devices, and systems involved in this application are merely illustrative examples and are not intended to require or imply that they must be connected, arranged, or configured in the manner shown in the block diagrams. As those skilled in the art will recognize, these devices, apparatuses, devices, and systems can be connected, arranged, and configured in any manner. Words such as “comprising,” “including,” “having,” etc., are open-ended terms meaning “including but not limited to,” and are used interchangeably with them. The terms “or” and “and” as used herein refer to the terms “and / or,” and are used interchangeably with them unless the context clearly indicates otherwise. The term “such as” as used herein refers to the phrase “such as but not limited to,” and is used interchangeably with it.

[0072] Additionally, as used herein, the “or” used in a list of items beginning with “at least one” indicates a separate list, such that a list of, for example, “at least one of A, B, or C” means A or B or C, or AB or AC or BC, or ABC (i.e., A and B and C). Furthermore, the word “exemplary” does not imply that the described example is preferred or better than other examples.

[0073] It should also be noted that in the apparatus and method of this application, the components or steps can be disassembled and / or recombined. These disassemblies and / or recombinations should be considered as equivalent solutions of this application.

[0074] It will be understood by those skilled in the art that all or any part of the methods and apparatus of this application can be implemented in hardware, firmware, software, or a combination thereof in any computing device (including processors, storage media, etc.) or network of computing devices. The hardware may be a general-purpose processor, digital signal processor (DSP), ASIC, field-programmable gate array (FPGA) or other programmable logic device (PLD), discrete gate or transistor logic, discrete hardware components, or any combination thereof designed to perform the functions described herein. The general-purpose processor may be a microprocessor, but alternatively, it may be any commercially available processor, controller, microcontroller, or state machine. The processor may also be implemented as a combination of computing devices, such as a combination of a DSP and a microprocessor, multiple microprocessors, one or more microprocessors cooperating with a DSP core, or any other such configuration. The software may reside in any form of computer-readable tangible storage medium. By way of example and not limitation, such computer-readable tangible storage media may include RAM, ROM, EEPROM, CD-ROM or other optical disc storage, disk storage or other magnetic storage devices, or any other tangible medium that can be used to carry or store desired program code in the form of instructions or data structures and is accessible by a computer. If used herein, the disks include compact discs (CDs), laser discs, optical discs, digital universal discs (DVDs), floppy disks, and Blu-ray discs.

[0075] Various changes, substitutions, and modifications can be made to the technology described herein without departing from the teachings defined by the appended claims. Furthermore, the scope of the claims is not limited to the specific aspects of the processes, machines, manufactures, events, means, methods, and actions described above. Currently existing or later-developed processes, machines, manufactures, events, means, methods, or actions that perform substantially the same function or achieve substantially the same result as the corresponding aspects described herein can be utilized. Therefore, the appended claims include such processes, machines, manufactures, events, means, methods, or actions within their scope.

[0076] The above description of the disclosed aspects is provided to enable any person skilled in the art to make or use this application. Various modifications to these aspects will be readily apparent to those skilled in the art, and the general principles defined herein can be applied to other aspects without departing from the scope of this application. Therefore, this application is not intended to be limited to the aspects shown herein, but rather to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0077] The above description has been given for illustrative and descriptive purposes. This description is not intended to limit the embodiments of this application to the forms disclosed herein. Although several exemplary aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, alterations, additions, and sub-combinations therein.

Claims

1. A positive pressure filtration method, comprising: Obtain the pressure range of different liquids during peptide desalting; Multiple filtration pressures are set according to the pressure range; Multiple corresponding filtration times are set according to the multiple filtration pressures; The solution is subjected to gradient filtration by switching the filtration pressure in the filtration column according to the filtration time.

2. The positive pressure filtration method as described in claim 1, wherein, Multiple filtration pressures can be set according to the pressure range, including: At least a first filtration pressure and a second filtration pressure are set, the first filtration pressure corresponds to a first filtration time, and the second filtration pressure corresponds to a second filtration time, wherein the first filtration pressure is greater than the second filtration pressure.

3. The positive pressure filtration method as described in claim 2, wherein, Setting multiple filtration pressures according to the aforementioned pressure range also includes: A third filtration pressure is set, which corresponds to a third filtration time. After the first filtration pressure and the second filtration pressure are applied, if liquid remains in part of the filter column, the third filtration pressure, the first filtration pressure, and the second filtration pressure are switched sequentially for the next round of filtration. The third filtration pressure is not less than the second filtration pressure.

4. The positive pressure filtration method as described in claim 3, wherein, The pressure ranges of different liquids during peptide desalting include: Test the pressure range of different media solutions in filter columns made of different materials.

5. The positive pressure filtration method as described in claim 4, wherein, The first filtering time t1 satisfies: 0.1s ≤ t1 ≤ 0.3s; The second filtration time t2 satisfies: 5s≤t2≤60s, and the third filtration time t3 satisfies: 0.1s≤t2≤10s.

6. The positive pressure filtration method as described in claim 1 or 5, wherein, Gradient filtration of the solution by switching the filtration pressure in the filtration column according to the filtration time includes: The solution is subjected to gradient filtration by cyclically switching the filtration pressure in the filtration column according to the filtration time.

7. The positive pressure filtration method as described in claim 6, wherein, Gradient filtration of the solution by cyclically switching the filtration pressure in the filter column according to the filtration time includes: the number of cycles being at least two.

8. A positive pressure filtration device, wherein, include: A positive pressure module is used to regulate and maintain the pressure within the filter column, allowing the solution to flow through the filter column and thus filter impurities from the solution; wherein the positive pressure module performs positive pressure filtration through the following method: Obtain the pressure range of different liquids during peptide desalting; Multiple filtration pressures are set according to the pressure range; The solution is filtered by switching the filtration pressure in the filter column according to the filtration time; A support assembly is disposed below the positive pressure module and is used to place the filter column; A drive assembly, connected to the positive pressure module, is used to drive the positive pressure module to move toward or away from the filter column.

9. The positive pressure filtration device as described in claim 8, wherein, The positive pressure module includes an upper positive pressure chamber and a lower positive pressure chamber. The upper positive pressure chamber is provided with an air inlet, and the bottom of the lower positive pressure chamber is provided with a positive pressure hole. A sealing gasket is provided on the bottom end face of the lower positive pressure chamber, and a through hole corresponding to the positive pressure hole is provided on the sealing gasket.

10. The positive pressure filtration device as claimed in claim 9, wherein, The drive assembly includes a pressure plate and drive components connected to both ends of the pressure plate. The upper positive pressure chamber is fixed on the pressure plate. The drive components include a lead screw and a motor.

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