A traditional Chinese medicine preparation for treating acne, a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine preparation for treating acne, a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine, and aims to provide a traditional Chinese medicine preparation for treating acne, which is prepared by comprehensively treating traditional Chinese medicine raw materials through microbial fermentation and alcohol extraction, maximally extracting effective components of the traditional Chinese medicine and decomposing harmful components, and adding excipients.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine preparation for acne treatment and skin care, a preparation method and application thereof. BACKGROUND

[0002] Acne treatment is the process of eliminating acne using medical technology or methods. Acne is the common name for acne, also known as "acne", "dark spots" or "pimples", which mainly occurs in adolescents and some young adults, and the incidence rate has been increasing in recent years. The clinical manifestations are characterized by multiple skin lesions such as facial acne, papules, pustules, nodules, etc. Not only does it cause discomfort to patients, but it also leaves scars, seriously affecting appearance, and may lead to psychological problems such as self-esteem, anxiety, depression, and affect the quality of life. Acne is related to a variety of factors, including excessive sebum secretion, follicle obstruction, bacterial infection and inflammatory response, etc. Therefore, acne treatment methods need to be developed to address multiple factors, increasing the difficulty of cure. In particular, after puberty, the hormone level in the human body increases rapidly, promoting the secretion of a large amount of sebum, causing duct obstruction, forming keratin plugs, i.e. acne, and a large number of harmful microorganisms proliferate and produce harmful substances, inducing and aggravating inflammatory response.

[0003] Currently, western medicine uses antibiotics, hormones and other drugs for treatment, which has large side effects and is prone to recurrence after drug withdrawal. Traditional Chinese medicine compositions have become an important direction for acne treatment because of their small side effects and long-lasting effects due to the absence of antibiotic, hormone and other components. Patent CN111803599B discloses a traditional Chinese medicine composition for acne treatment and its preparation, which mixes Zhebeimu, Lianqiao, Tianhuafen, calcined gypsum, Shengdifyu, Baiying, Huashi, Bai'an, Baiji and Yiyiren powder, then soaks in cold water, boils and extracts, filters and concentrates, and adds yogurt or tea and other accessories. This method has defects: the traditional Chinese medicine preparation prepared by simply mixing and extracting traditional Chinese medicine components has less effective components, poor absorption, and is prone to leave harmful components in traditional Chinese medicine. In addition, the conventional boiling extraction method can easily destroy the effective components in traditional Chinese medicine. Therefore, there is an urgent need to develop a traditional Chinese medicine preparation for acne treatment and skin care with better effect, smaller side effect, long-lasting effect and no irritation to better solve the problem of acne. SUMMARY

[0004] The present application aims to provide a traditional Chinese medicine preparation for acne treatment and skin care, a preparation method and application thereof. The traditional Chinese medicine raw materials are treated by microbial fermentation and alcohol extraction to maximize the extraction of effective components and decomposition of harmful components in traditional Chinese medicine, which can effectively inhibit harmful microorganisms, repair skin tissue and reduce recurrence risks to treat acne from multiple dimensions. The preparation can be applied to the affected area to directly reach the lesion, has good acne treatment effect, safe components, no toxic side effects, high cure rate, long-lasting effect, no irritation and other characteristics.

[0005] To achieve the above object, the present application provides a traditional Chinese medicine preparation for acne treatment and skin care, comprising the following traditional Chinese medicine raw materials by weight: Gleditsia sinensis 24-28 parts, Spermacoce latifolia 12-14 parts, Oniscus asellus 12-14 parts, raw rhubarb 12-14 parts, golden cypress 12-14 parts, Sophora flavescens 12-14 parts, Atractylodes lancea 12-14 parts, gentian 12-14 parts, radix angelicae 12-14 parts, pokeweed 12-14 parts, Kaempferia rotunda 12-14 parts, gallnut 12-14 parts, camphor 8-10 parts, borneol 1-2 parts, and licorice 1-2 parts.

[0006] The present application also provides a preparation method of the traditional Chinese medicine preparation for acne treatment and skin care, comprising the following steps:

[0007] S1, traditional Chinese medicine pretreatment: taking the traditional Chinese medicine raw materials, mixing uniformly, adding to a fermentation tank, adding 75% ethanol and stirring uniformly, stirring and treating to volatilize ethanol, until the ethanol content is reduced to 50%±5%, to obtain a traditional Chinese medicine pretreatment liquid;

[0008] S2, microbial fermentation: adding purified water to the traditional Chinese medicine pretreatment liquid, stirring uniformly, inoculating a compound microbial starter for fermentation, adding a feeding liquid during the fermentation process, and obtaining a traditional Chinese medicine fermentation liquid after fermentation;

[0009] S3, extraction: filtering and extracting the traditional Chinese medicine fermentation liquid to obtain a filtrate and a residue;

[0010] S4, post-treatment: mixing the filtrate uniformly, adding excipients and stirring uniformly, filling into bottles, and obtaining the traditional Chinese medicine preparation for acne treatment and skin care.

[0011] Further, the traditional Chinese medicine raw materials in S1 contain the traditional Chinese medicine raw materials.

[0012] Optionally, the mass ratio of the traditional Chinese medicine raw materials to 75% ethanol in S1 is 1:(1-1.1).

[0013] Optionally, the stirring speed in S1 is 40-50 rpm, and the stirring time is 23-24 h.

[0014] Further, the mass ratio of the traditional Chinese medicine pretreatment liquid to purified water in S2 is 1:(9-9.5).

[0015] Optionally, the compound microbial starter in S2 comprises Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti; preferably, the inoculation amount of Saccharomyces cerevisiae and Bacillus amyloliquefaciens is 0.8-0.84%, and the inoculation amount of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti is 0.4-0.42%.

[0016] Optionally, the fermentation time of the compound microbial fermentation in S2 is 70-72 h.

[0017] Optionally, the feed solution in S2 comprises the following components and mass contents: glucose 48-50%, proteose peptone 4.8-5%, cellulase 0.48-0.5%, pectinase 0.48-0.5%, papain 0.95-1%, and the balance is distilled water.

[0018] Optionally, the feed solution in S2 is added by flow feeding through the feed port; preferably, the flow feeding is performed once per hour, and the volume ratio of each feeding amount is 0.14-0.15%.

[0019] Further, the filtration extraction in S3 is a multi-stage extraction method with different concentrations of ethanol; preferably, the filtration extraction in S3 is a three-stage extraction method with different concentrations of ethanol.

[0020] Optionally, the three-stage extraction method with ethanol in S3 comprises the following steps:

[0021] First, the traditional Chinese medicine fermentation liquor obtained in S2 is filtered through defatted cotton gauze to obtain juice, a first-pass filtrate and a first-pass residue, 95% ethanol is added to the first-pass residue, and after stirring and extraction, a first-pass extract is obtained;

[0022] Then, the first-pass extract is filtered through defatted cotton gauze to obtain juice, a second-pass filtrate and a second-pass residue, 50% ethanol is added to the second-pass residue, a feed solution containing 4.8-5% snail proteinase is added through the feed port for flow feeding, and after stirring and extraction, a second-pass extract is obtained;

[0023] Finally, the second-pass extract is subjected to pressing treatment, filtered through defatted cotton gauze to obtain juice, a third-pass filtrate, and the third-pass residue is discarded or retained.

[0024] Optionally, the first-pass residue and 95% ethanol are in a mass ratio of 1:(1-1.1), and the stirring and extraction time is 48-50h.

[0025] Optionally, the second-pass residue and 50% ethanol are in a mass ratio of 1:(0.9-1), and the stirring and extraction time is 16-18h.

[0026] Optionally, the flow feeding is performed once every 10 minutes, and the volume ratio of each feeding amount is 0.1-0.12%.

[0027] Furthermore, the excipients in S4 include the following components and weight percentages: linseed oil 1-1.2%, lecithin 1-1.2%, cholesterol-5-en-3β-ol 0.24-0.25%, lemon juice 4-4.8%, lauryl glucoside 0.48-0.5%, disodium EDTA 0.018-0.02%, sodium dihydrogen phosphate 0.45-0.46%, and disodium hydrogen phosphate 0.04-0.05%.

[0028] In addition, the present invention also provides the application of the traditional Chinese medicine preparation for acne treatment and skin care, or the traditional Chinese medicine preparation for acne treatment and skin care prepared by the method, in the preparation of acne treatment and skin care drugs.

[0029] This invention relates to a traditional Chinese medicine preparation for acne treatment and skin care, primarily prepared by comprehensively processing raw materials through microbial fermentation and ethanol extraction, and adding excipients. The main preparation method and principle are as follows: Raw materials are mixed evenly, and 75% ethanol is added and stirred until homogeneous. This serves two purposes: firstly, the bactericidal function of 75% ethanol effectively eliminates unwanted bacteria to prevent their influence during subsequent fermentation; secondly, the 75% ethanol pre-extracts the raw materials to promote the subsequent fermentation process. Then, purified water is added to dilute the ethanol concentration, followed by the addition of a compound microbial fermentation agent for inoculation and fermentation. Microbial fermentation decomposes macromolecular and harmful components. After fermentation, the effective components are extracted in multiple stages using ethanol of different concentrations. Due to the decomposition of cell walls by microorganisms and enzymes, a large amount of intracellular substances are released, resulting in higher extraction efficiency. The staged extraction is primarily to allow the addition of snail protease in the second stage of extraction to assist in the extraction process. A low-concentration ethanol solution with continuous addition of snail protease is used to prevent the snail protease from becoming inactive, as excessively high ethanol concentrations can lead to snail protease inactivation. Finally, excipients are added to the filtrate and stirred evenly. After ultraviolet sterilization and bottling, the finished product is obtained.

[0030] By adopting the above technical solution, the present invention has the following beneficial effects:

[0031] 1. The main Chinese herbal raw materials used in the preparation of this invention contain Gleditsia sinensis thorns, Paederia scandens, Armadillidium vulgare, Rheum palmatum, Phellodendron chinense, Sophora flavescens, Atractylodes lancea, Gentiana scabra, Angelica dahurica, Phytolacca acinosa, Kaempferia galanga, Galla chinensis, Camphor, Borneol, and Glycyrrhiza uralensis. Among them, Gleditsia sinensis thorns have the effects of reducing swelling and draining pus, and killing parasites; Paederia scandens, Armadillidium vulgare, and Rheum palmatum all have the effects of clearing heat and detoxifying, and can also resolve blood stasis, relieve pain, and regulate menstruation; Phellodendron chinense, Sophora flavescens, Atractylodes lancea, Gentiana scabra, and Angelica dahurica all have the effects of clearing heat and drying dampness, and can also dispel wind, heal sores, reduce swelling and drain pus; Phytolacca acinosa detoxifies and disperses nodules; Kaempferia galanga removes dampness and relieves pain; Galla chinensis astringes dampness and heals sores; Camphor and Borneol have the effects of clearing heat, relieving pain, reducing swelling and relieving itching; Glycyrrhiza uralensis can harmonize the effects of all the herbs. The combined effects of these methods can regulate sebum secretion, inhibit harmful microorganisms, and repair skin tissue, without irritation or toxic side effects, making the acne treatment and skin care process gentler and safer.

[0032] 2、The pre-treatment enzyme in the application contains cellulase, pectinase, papain, and the post-treatment enzyme is snail protease, which can maximize the extraction of effective components of traditional Chinese medicinal materials and decompose harmful components, and can also promote skin repair and regeneration, eliminate scars, and naturally kill bacteria, directly reaching the lesion and repairing skin damage from the root. Among them, cellulase and pectinase are used to decompose the cell wall of traditional Chinese medicinal materials and promote the release of intracellular substances; papain is a multi-enzyme system extracted from unripe fruits of papaya without purification, which has high protease and lipase activity and can hydrolyze macromolecular proteins and part of fats into amino acids and fatty acids that are easy to absorb and utilize; snail protease is the essence extracted from snail meat and mucus, containing allantoin, calcium, glycolic acid, bone collagen, protein and vitamins and other components, and the applicant has found through a large number of experiments that it can be extracted with low-concentration ethanol, which can not only hydrolyze various macromolecular components and harmful components synchronously in the post-treatment process, but also promote the hydrolysis of collagen and elastin in skin tissue and has the effects of promoting skin repair and regeneration and antioxidant. At the same time, the application adopts multi-stage extraction of effective components with different concentrations of ethanol after fermentation, and adds snail protease into the fermentation broth, so that the hydrolysis of snail protease and the extraction of ethanol are synchronized, which not only improves the extraction efficiency, but also preserves part of the snail protease with active function for subsequent clinical application.

[0033] 3、The fermentation bacteria in the application contain Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum and Acetobacter aceti, which are synergistically fermented through different metabolic processes of different strains, can not only assist in reducing the ethanol concentration to promote the growth and metabolism of beneficial bacteria, but also form a bacterial film to inhibit harmful microorganisms, effectively promote the decomposition of macromolecular components and harmful components of traditional Chinese medicinal materials, and can also metabolize a variety of components beneficial to the skin, thereby improving the use effect of the traditional Chinese medicinal materials used in the application. Among them, Saccharomyces cerevisiae belongs to the genus Saccharomyces, which mainly plays a role in converting sugar into ethanol, and consumes oxygen in the fermentation broth to prevent the growth of miscellaneous bacteria; Bacillus amyloliquefaciens is a bacterium with high genetic similarity to Bacillus subtilis, which is named for its ability to produce various amylases and proteases, and it can efficiently metabolize and decompose the components in traditional Chinese medicinal materials; Lactobacillus plantarum and Lactobacillus acidophilus belong to the genus Lactobacillus in Lactobacillus, and Bifidobacterium longum belongs to the genus Bifidobacterium, all of which can metabolize a large amount of organic acids and bacteriocins, which can effectively inhibit miscellaneous bacteria and harmful bacteria; Acetobacter aceti belongs to the genus Acetobacter, which can not only convert ethanol into acetic acid, but also has the ability to metabolize other alcohols and sugars into a variety of organic acids beneficial to the skin, and can also metabolize ethanol, which has a mutualistic symbiotic relationship with Saccharomyces. In addition, as an aerobic bacterium, it can form a layer of white bacterial film on the surface during the fermentation process, which can effectively prevent the invasion of oxygen and harmful bacteria into the fermentation broth.

[0034] 4. The excipients in this invention contain linseed oil, lecithin, cholesterol-5-en-3β-ol, lemon juice, lauryl glucoside, disodium EDTA, sodium dihydrogen phosphate, and disodium hydrogen phosphate. These ingredients can further enhance the absorption and efficacy of traditional Chinese medicine and improve the stability of the preparation. They can also synergistically moisturize, reduce inflammation, soften keratin, and prevent recurrence, resulting in a long-lasting effect. Flaxseed oil contains unsaturated fatty acids such as alpha-linolenic acid and linoleic acid, which can prevent skin inflammation and moisturize the skin. It can also inhibit 5α-reductase, a major contributing factor to acne formation. Additionally, flaxseed oil contains vitamin E, which can promote skin metabolism and soften the stratum corneum. Lecithin and cholesterol-5-en-3β-ol both act as oil emulsifiers, enhancing each other's binding effect with water-soluble components and promoting the mixing of the aqueous and oil phases in the formulation. Both are also important components of the cell membrane; when they encapsulate and bind the active ingredients in the formulation, they allow the active ingredients to penetrate the skin barrier more efficiently and exert their effects. Lemon juice contains abundant vitamin C and citric acid, with vitamin C possessing strong antioxidant properties. It can significantly prevent the oxidation of unsaturated fatty acids in linseed oil, thereby inhibiting 5α-reductase. Citric acid softens the stratum corneum. Combined with organic components such as ethanol in the formulation, it can dissolve and remove oil and epidermal tissue clogging pores. Lauryl glucoside mainly acts as a surfactant. Through its wetting, emulsifying, and solubilizing effects, it helps the active ingredients in the formulation to have close contact with the skin surface, increasing absorption. In addition, it has the best penetration-enhancing effect among alkyl glycosides and is also relatively mild. Sodium dihydrogen phosphate and disodium hydrogen phosphate are pH buffer pairs, which stabilize the pH. Disodium EDTA mainly acts as a chelating agent and stabilizer. It can form chelates with metal ions in the formulation to improve the stability of the formulation. At the same time, it works with the buffer pairs to further slow down the pH changes of the formulation and stabilize the quality of the formulation. Detailed Implementation

[0035] To further explain the technical solution of the present invention, the present invention will be described in detail below through specific embodiments.

[0036] This invention provides a traditional Chinese medicine preparation for acne treatment and skin care, comprising the following parts by weight of traditional Chinese medicine raw materials: 24-28 parts of Gleditsia sinensis thorns, 12-14 parts of Paederia scandens, 12-14 parts of Armadillidium vulgare, 12-14 parts of raw rhubarb, 12-14 parts of Phellodendron chinense, 12-14 parts of Sophora flavescens, 12-14 parts of Atractylodes lancea, 12-14 parts of Gentiana scabra, 12-14 parts of Angelica dahurica, 12-14 parts of Phytolacca acinosa, 12-14 parts of Kaempferia galanga, 12-14 parts of Galla chinensis, 8-10 parts of camphor, 1-2 parts of borneol, and 1-2 parts of licorice.

[0037] The application further provides a preparation method of the traditional Chinese medicine preparation for acne treatment and skin care, which comprises the following steps:

[0038] S1, traditional Chinese medicine material pretreatment: taking traditional Chinese medicine raw materials, uniformly mixing, adding into a fermentation tank, adding 75% ethanol, uniformly stirring, stirring and treating to volatilize ethanol, until the content of ethanol is reduced to 50%±5%, to obtain a traditional Chinese medicine material pretreatment liquid;

[0039] S2, microbial fermentation: adding purified water into the traditional Chinese medicine material pretreatment liquid, uniformly stirring, inoculating a compound microbial starter for fermentation, adding a feeding liquid during the fermentation, and obtaining a traditional Chinese medicine fermentation liquid after the fermentation;

[0040] S3, extraction: filtering and extracting the traditional Chinese medicine fermentation liquid, to obtain a filtrate and a residue;

[0041] S4, post-treatment: uniformly mixing the filtrate, adding excipients and uniformly stirring, filling into bottles, to obtain the traditional Chinese medicine preparation for acne treatment and skin care.

[0042] Further, the traditional Chinese medicine raw materials in S1 contain the traditional Chinese medicine raw materials.

[0043] Optionally, the mass ratio of the traditional Chinese medicine raw materials to 75% ethanol in S1 is 1:(1-1.1).

[0044] Optionally, the stirring speed in S1 is 40-50 rpm, and the stirring time is 23-24 h.

[0045] Further, the mass ratio of the traditional Chinese medicine material pretreatment liquid to purified water in S2 is 1:(9-9.5).

[0046] Optionally, the compound microbial starter in S2 comprises Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum and Acetobacter aceti; preferably, the inoculation amount of Saccharomyces cerevisiae and Bacillus amyloliquefaciens is 0.8-0.84%, and the inoculation amount of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum and Acetobacter aceti is 0.4-0.42%.

[0047] Optionally, the fermentation time of the compound microbial fermentation in S2 is 70-72 h.

[0048] Optionally, the feeding liquid in S2 comprises the following components and mass contents: 48-50% glucose, 4.8-5% protein peptone, 0.48-0.5% cellulase, 0.48-0.5% pectinase, 0.95-1% papain, and the balance is distilled water.

[0049] Optionally, the feeding liquid in S2 is fed through the feeding port.

[0050] Further, the filtering extraction in S3 is a multi-stage extraction method with different concentrations of ethanol.

[0051] Optionally, the three-stage extraction method with ethanol in S3 comprises the following steps:

[0052] Firstly, the traditional Chinese medicine fermentation liquid obtained in S2 is filtered through the defatted cotton gauze to obtain juice, the first filtering liquid and the first residue, 95% ethanol is added to the first residue, and the first extraction liquid is obtained after stirring and extraction;

[0053] Then, the first extraction liquid is filtered through the defatted cotton gauze to obtain juice, the second filtering liquid and the second residue, 50% ethanol is added to the second residue, the feeding liquid containing 4.8-5% snail proteinase is fed through the feeding port, and the second extraction liquid is obtained after stirring and extraction;

[0054] Finally, the second extraction liquid is subjected to squeezing treatment, filtered through the defatted cotton gauze to obtain juice, and the third filtering liquid is obtained, and the third residue is discarded or reserved.

[0055] Optionally, the first residue and 95% ethanol are stirred according to the mass ratio of 1:(1-1.1), and the stirring extraction time is 48-50h.

[0056] Optionally, the second residue and 50% ethanol are stirred according to the mass ratio of 1:(0.9-1), and the stirring extraction time is 16-18h.

[0057] Optionally, the feeding method is feeding every 10 minutes, and the volume ratio of each feeding amount is 0.1-0.12%.

[0058] Further, the auxiliary material in S4 comprises the following components and weight percentage: 1-1.2% of linseed oil, 1-1.2% of lecithin, 0.24-0.25% of cholesta-5-ene-3β-ol, 4-4.8% of lemon juice, 0.48-0.5% of lauryl glucoside, 0.018-0.02% of ethylenediaminetetraacetic acid disodium salt, 0.45-0.46% of sodium dihydrogen phosphate, and 0.04-0.05% of disodium hydrogen phosphate.

[0059] In addition, the application also provides an application of the acne-removing and skin-protecting traditional Chinese medicine preparation or the acne-removing and skin-protecting traditional Chinese medicine preparation prepared by the method in the acne-removing and skin-protecting medicine.

[0060] The preferred embodiments of the present application are described in detail below, which are intended to explain the present application, and cannot be understood as a limitation of the present application. The specific techniques or conditions not specified in the examples are carried out according to the techniques or conditions described in the literature in the art or according to the product instructions. The reagents or instruments not specified by the manufacturer are all conventional products that can be obtained by purchase. In the following examples, unless otherwise specified, "%" refers to the percentage by weight.

[0061] The main raw materials used in the embodiments of the present application are as follows, but are not limited to the following raw materials:

[0062] The traditional Chinese medicine raw materials are purchased from Anhui Hejingtang Chinese Herbal Pieces Co., Ltd.

[0063] The compound microbial starter is purchased from Jiangsu Jiujia Biological Technology Co., Ltd.

[0064] Glucose, proteose peptone, sodium dihydrogen phosphate, and disodium hydrogen phosphate are purchased from Beijing Lujiaotong Technology Co., Ltd.

[0065] Cellulase, pectinase, and papain are purchased from Nanning Dongheng Huadao Biological Technology Co., Ltd.

[0066] Snail proteinase is purchased from Nanjing Wobio Biological Technology Co., Ltd.

[0067] Linseed oil is purchased from Heze Zhonghe Jianyuan Biological Technology Co., Ltd.

[0068] Lecithin and cholesta-5-ene-3β-ol are purchased from Wuhan Kemik Biological Medicine Technology Co., Ltd.

[0069] Lauryl glucoside and disodium ethylenediaminetetraacetate are purchased from Wuhan Jixin Yibang Biological Technology Co., Ltd.

[0070] The preparation method of lemon juice is as follows: after the lemon is squeezed, the impurities are filtered out by defatted cotton gauze, and the lemon juice is obtained.

[0071] The ethanol content is detected by a simple alcohol meter.

[0072] Example 1

[0073] S1, Pretreatment of Traditional Chinese Medicine Raw Materials

[0074] The traditional Chinese medicine raw materials are weighed, ground to 120 mesh, and then mixed uniformly. Then 75% ethanol is added to the fermentation tank according to the mass ratio of traditional Chinese medicine raw materials to 75% ethanol of 1:1.1. The mixture is soaked and stirred, and then heated to 65°C to volatilize the ethanol. After the ethanol content is reduced to 50%±5%, the pretreated traditional Chinese medicine raw material solution is obtained.

[0075] The traditional Chinese medicine raw materials comprise the following components and mass contents: Gleditsia sinensis 28 parts, Sageretia theezans 12 parts, Oniscus asellus 14 parts, raw rhubarb 14 parts, Phellodendron amurense 12 parts, Sophora flavescens 14 parts, Atractylodes lancea 12 parts, Gentiana scabra 14 parts, Angelica dahurica 12 parts, Diodia virginiana 14 parts, Kaempferia rotunda 12 parts, gallnut 14 parts, camphor 8 parts, borneol 1 part, and licorice 2 parts.

[0076] The stirring treatment is performed at a rotation speed of 50 rpm for 24 h, and other parameters are not controlled during the process.

[0077] S2, microbial fermentation

[0078] The purified water is added to the traditional Chinese medicine pretreatment liquid at a mass ratio of 1:9, and stirred uniformly, and then inoculated with Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti for fermentation. During the fermentation process, the feed liquid is added through the feeding port for flow feeding. After 72 h of fermentation, the traditional Chinese medicine fermentation liquid is obtained.

[0079] The inoculation amount of Saccharomyces cerevisiae and Bacillus amyloliquefaciens is 0.8%.

[0080] The inoculation amount of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti is 0.42%.

[0081] The feed liquid comprises the following components and mass contents: glucose 50%, proteose peptone 4.8%, cellulase 0.5%, pectinase 0.48%, papain 1%, and the balance is distilled water.

[0082] The feeding frequency and amount are as follows: feeding once every hour, and the volume ratio of the amount of each feeding is 0.15%.

[0083] S3, extraction

[0084] First, the traditional Chinese medicine fermentation liquid is filtered through the defatted cotton gauze to obtain the first filtration liquid and the first residue. The 95% ethanol is added to the first residue at a mass ratio of 1:1.1, and stirred for 48 h to obtain the first extraction liquid.

[0085] Then, the first extraction liquid is filtered through the defatted cotton gauze to obtain the second filtration liquid and the second residue. The 50% ethanol is added to the second residue at a mass ratio of 90%, and the feed liquid containing 5% snail proteinase aqueous solution is added through the feeding port for flow feeding. After stirring for 18 h, the second extraction liquid is obtained.

[0086] Finally, the second extraction liquid is subjected to squeezing treatment, filtered through the defatted cotton gauze to obtain the third filtration liquid, and the third residue is discarded or reserved.

[0087] The replenishment frequency and amount are as follows: replenish once every 10 minutes, with each replenishment amount accounting for 0.1% of the total volume.

[0088] S4, Post-processing

[0089] The first, second, and third filtrates are mixed evenly, excipients are added and stirred evenly, and then sterilized with ultraviolet light and bottled to obtain the finished product, which is a traditional Chinese medicine preparation for acne treatment and skin care.

[0090] The amount of excipients added is 7.658% of the total liquid weight after mixing the above-mentioned filtrate. Specifically, the excipients include the following components and their weight percentages: linseed oil 1.2%, lecithin 1.2%, cholesterol-5-en-3β-ol 0.24%, lemon juice 4%, lauryl glucoside 0.5%, disodium EDTA 0.018%, sodium dihydrogen phosphate 0.46%, and disodium hydrogen phosphate 0.04%.

[0091] Example 2

[0092] The difference between this embodiment and embodiment 1 is that the Chinese herbal raw materials in S1 include the following components and their mass content: 24 parts of Gleditsia sinensis thorns, 14 parts of Paederia scandens, 12 parts of Armadillidium vulgare, 12 parts of raw rhubarb, 14 parts of Phellodendron chinense, 12 parts of Sophora flavescens, 14 parts of Atractylodes lancea, 12 parts of Gentiana scabra, 14 parts of Angelica dahurica, 12 parts of Phytolacca acinosa, 14 parts of Kaempferia galanga, 12 parts of Galla chinensis, 10 parts of camphor, 2 parts of borneol, and 1 part of licorice.

[0093] Example 3

[0094] The difference between this embodiment and Embodiment 1 is that the Chinese herbal raw materials in S1 include the following components and their mass content: 26 parts of Gleditsia sinensis thorns, 13 parts of Paederia scandens, 13 parts of Armadillidium vulgare, 13 parts of raw rhubarb, 13 parts of Phellodendron chinense, 13 parts of Sophora flavescens, 13 parts of Atractylodes lancea, 13 parts of Gentiana scabra, 13 parts of Angelica dahurica, 13 parts of Phytolacca acinosa, 13 parts of Kaempferia galanga, 13 parts of Galla chinensis, 9 parts of camphor, 1.5 parts of borneol, and 1.5 parts of licorice.

[0095] Example 4

[0096] The difference between this embodiment and Embodiment 1 is that in S2, the inoculation amounts of Saccharomyces cerevisiae and Bacillus amyloliquefaciens are both 0.84%, while the inoculation amounts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacterium are all 0.4%.

[0097] Example 5

[0098] The difference between this embodiment and Embodiment 1 is that in S2, the inoculation amounts of Saccharomyces cerevisiae and Bacillus amyloliquefaciens are both 0.82%, while the inoculation amounts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacterium are all 0.41%.

[0099] Example 6

[0100] The difference between this embodiment and Embodiment 1 is that the feed solution in S2 is: 48% glucose, 5% peptone, 0.48% cellulase, 0.5% pectinase, 0.95% papain, and the remainder is distilled water.

[0101] Example 7

[0102] The difference between this embodiment and Embodiment 1 is that the feed solution in S2 is: 49% glucose, 4.9% peptone, 0.49% cellulase, 0.49% pectinase, 0.97% papain, and the remainder is distilled water.

[0103] Example 8

[0104] The difference between this embodiment and Embodiment 1 is that the feed solution in S3 is a 4.8% snail protease aqueous solution.

[0105] Example 9

[0106] The difference between this embodiment and Embodiment 1 is that the feed solution in S3 is a 4.9% snail protease aqueous solution.

[0107] Example 10

[0108] The difference between this embodiment and Example 1 is that the excipients in S4 include the following components and weight percentages: 1% linseed oil, 1% lecithin, 0.25% cholesterol-5-en-3β-ol, 4.8% lemon juice, 0.48% lauryl glucoside, 0.02% disodium EDTA, 0.45% sodium dihydrogen phosphate, and 0.05% disodium hydrogen phosphate.

[0109] Comparative Example 1

[0110] The difference between this comparative example and Example 1 is that the S1 pretreatment of Chinese medicinal materials and the S2 microbial fermentation were not performed. These steps were replaced with:

[0111] Weigh the Chinese medicinal materials, grind them into powder to 120 mesh, mix them evenly and add them to the fermentation tank. Mix the Chinese medicinal materials, 50% ethanol and purified water evenly according to the mass ratio of 1:1:18 to obtain the Chinese medicinal material pretreatment solution.

[0112] The Chinese medicinal materials include the following components and their mass content: 28 parts of Gleditsia sinensis thorns, 12 parts of Paederia scandens, 14 parts of Armadillidium vulgare, 14 parts of raw rhubarb, 12 parts of Phellodendron chinense, 14 parts of Sophora flavescens, 12 parts of Atractylodes lancea, 14 parts of Gentiana scabra, 12 parts of Angelica dahurica, 14 parts of Phytolacca acinosa, 12 parts of Kaempferia galanga, 14 parts of Galla chinensis, 8 parts of camphor, 1 part of borneol, and 2 parts of licorice.

[0113] Comparative Example 2

[0114] The difference between this comparative example and Example 1 is that the pretreatment of the medicinal materials in S1 was not performed; this step was replaced with:

[0115] The traditional Chinese medicine raw materials are weighed, ground to 120 mesh, and then mixed uniformly and added to a fermentation tank. 50% ethanol is added according to a mass ratio of 1:1 to obtain a traditional Chinese medicine pretreatment liquid.

[0116] The traditional Chinese medicine raw materials include the following components and mass contents: Gleditsia sinensis 28 parts, Sageretia theezans 12 parts, Dioxia oppositifolia 14 parts, Radix et Rhizoma Polygoni 12 parts, Rhizoma et Radix Rhei 14 parts, camphor 8 parts, borneol 1 part, and Oniscus asellus 14 parts.

[0117] Comparative Example 3

[0118] The difference between this comparative example and Example 1 is that the S2 microbial fermentation is not performed, and this step is replaced by:

[0119] The traditional Chinese medicine pretreatment liquid and purified water are added to the purified water according to a mass ratio of 1:9, and then stirred uniformly to obtain a traditional Chinese medicine pretreatment liquid.

[0120] Comparative Example 4

[0121] The difference between this comparative example and Example 1 is that the acetic acid bacillus in the fermentation bacteria in S2 is replaced by Lactobacillus rhamnosus.

[0122] Comparative Example 5

[0123] The difference between this comparative example and Example 1 is that glucose and protein peptone are not added to the feeding liquid in S2.

[0124] Comparative Example 6

[0125] The difference between this comparative example and Example 1 is that cellulase, pectinase, and papain are not added to the feeding liquid in S2.

[0126] Comparative Example 7

[0127] The difference between this comparative example and Example 1 is that the second immersion extraction process is not performed in S3, and only an equal amount of 50% ethanol is added, and other conditions remain unchanged.

[0128] Comparative Example 8

[0129] The difference between this comparative example and Example 1 is that the second extraction liquid is not obtained by filtering in S3, and the snail proteinase feeding is performed in the first filtering extraction, and other conditions remain unchanged.

[0130] Comparative Example 9

[0131] The difference between this comparative example and Example 1 is that the feeding liquid is not flowed and added during the second extraction in S3.

[0132] Comparative Example 10

[0133] The difference between this comparative example and Example 1 is that lemon juice is not added to the adjuvant in S4, and linseed oil is replaced by cottonseed oil.

[0134] Comparative Example 11

[0135] The difference between this comparative example and Example 1 is that linseed oil is replaced by cottonseed oil in the adjuvant in S4.

[0136] Comparative Example 12

[0137] The difference between this comparative example and Example 1 is that lemon juice is not added to the adjuvant in S4.

[0138] Comparative Example 13

[0139] The difference between this comparative example and Example 1 is that lauryl glucoside is replaced by glycerol ester in the adjuvant in S4.

[0140] Comparative Example 14

[0141] The difference between this comparative example and Example 1 is that cholesta-5en-3β-ol is not added to the adjuvant in S4, and lecithin is replaced by triglyceride.

[0142] Comparative Example 15

[0143] The difference between this comparative example and Example 1 is that lecithin is replaced by triglyceride in the adjuvant in S4.

[0144] Comparative Example 16

[0145] The difference between this comparative example and Example 1 is that cholesta-5en-3β-ol is not added to the adjuvant in S4.

[0146] Bacteriostatic rate effect test

[0147] (1) Preparation of samples

[0148] The traditional Chinese medicine preparations obtained in Examples 1-10 and Comparative Examples 1-9 above were taken as experimental groups and control groups, respectively.

[0149] An aqueous solution containing 0.5% chlorhexidine acetate was taken as a competitive control group.

[0150] 0.03 mol / L phosphate buffer solution (PBS) (pH = 7.2-7.4) was taken as a positive control group.

[0151] (2) Bacteriostatic test

[0152] The bacteriostasis rate detection is based on the suspension quantitative bacteriostasis test in 5.1.1 of WS / T 650-2019 "Antibacterial and Bacteriostasis Effect Evaluation Method", the indicator bacteria are Staphylococcus aureus (ATCC 6538) and Propionibacterium acnes (ATCC 6919), the action time is 1 min, and the calculation formula of the bacteriostasis rate is as follows:

[0153] Bacteriostasis rate (%) = (bacterial amount (cfu / mL) of the positive control group - bacterial amount (cfu / mL) of the sample group) / bacterial amount (cfu / mL) of the positive control group * 100%;

[0154] The bacteriostasis test results are shown in Table 1.

[0155] Table 1: Bacteriostasis rate table of each sample group

[0156]

[0157]

[0158] As can be seen from the comparison data of Examples 1-10 and the competitor control group in Table 1, compared with the conventional 0.5% chlorhexidine acetate aqueous solution on the market, the bacteriostasis effect of the traditional Chinese medicine preparation of the present application is better.

[0159] As can be seen from Comparative Examples 1-3, without pre-treatment and / or without fermentation will cause a significant decrease in the bacteriostasis rate, and only pre-treatment or only fermentation has a very limited improvement on the bacteriostasis rate compared with the simultaneous pre-treatment and fermentation of the examples, and the improvement effect of fermentation alone on the bacteriostasis rate is slightly higher than that of pre-treatment alone, which may be because the main role of pre-treatment is to pre-extract traditional Chinese medicinal materials before fermentation for subsequent strain fermentation, and at the same time, the pre-treatment can kill the miscellaneous bacteria in the traditional Chinese medicinal materials by 75% ethanol, which can also prevent the interference of miscellaneous bacteria in the fermentation process.

[0160] As can be seen from Comparative Example 4, replacing Acetobacter during the fermentation process with the more conventional Lactobacillus rhamnosus in the genus Lactobacillus will cause a significant decrease in the bacteriostasis rate, which is because Acetobacter not only can metabolize ethanol to produce acetic acid to promote the fermentation process, but also can produce a bacterial membrane to prevent the growth of miscellaneous bacteria and thus improve the fermentation effect.

[0161] As can be seen from Comparative Examples 5-6, removing the fermentation substrate and / or the pre-treatment enzyme in the fermentation feed liquid will cause a significant decrease in the bacteriostasis rate, which is because the fermentation substrate and / or the pre-treatment enzyme can promote the fermentation process, the former provides carbon and nitrogen sources for the fermentation strain, and the latter can hydrolyze the cell wall of traditional Chinese medicinal materials, which is beneficial to the utilization of intracellular substances of traditional Chinese medicinal materials by microorganisms.

[0162] As can be seen from Comparative Examples 7-8, in the subsequent multi-stage extraction, only the first stage extraction results in a certain decrease in the antibacterial rate, and only the first stage extraction with additional flow addition of snail proteinase in the first stage extraction has little effect on the antibacterial rate, because the second stage extraction is mainly combined with snail proteinase to extract traditional Chinese medicinal materials, and too high concentration of ethanol can cause snail proteinase to be almost completely inactivated, losing its effect.

[0163] At the same time, as can be seen from Comparative Example 9, the flow addition of snail proteinase has a very obvious effect on the improvement of the antibacterial rate.

[0164] 5α-reductase inhibition rate effect test

[0165] (1) Preparation of samples

[0166] The traditional Chinese medicinal preparations obtained in Examples 1-10 and Comparative Examples 1-12 above were taken as experimental groups and control groups, respectively.

[0167] No sample was added, which was taken as a blank control group.

[0168] (2) 5α-reductase inhibition rate test

[0169] The 5α-reductase inhibition rate was calculated by absorbance determination, and the specific determination method was as follows: testosterone T, 5α-reductase, phosphate buffer (pH = 7.4), and reduced coenzyme II tetrasodium salt were added to a reaction tube, mixed, and then the absorbance was determined at a wavelength of 340 nm. After 60 min of constant temperature incubation at 37℃, the absorbance was determined.

[0170] The reagent amounts of the reaction system for inhibition rate test of each sample group are shown in Table 2, and the units of the reagent amounts in the table are μL.

[0171] Table 2 Reagent amounts of the reaction system for inhibition rate test of each sample group

[0172]

[0173] The calculated difference of the experimental group and the control group with the sample added is the sample decrease value A n , and the calculated difference of the blank control group without the sample added is the blank decrease value A0.

[0174] The size of the inhibition rate was calculated, and the calculation formula was as follows: inhibition rate / % = (A 0- A n ) / A0x 100%.

[0175] The preparation method of 5α-reductase enzyme solution is as follows: taking a mouse, killing by cervical dislocation, dissecting the liver under low temperature, homogenizing with pre-cooled buffer (sucrose 0.32 mol / L, DTT 0.1 mmol / L, sodium phosphate 20 mmol / L, pH 6.5) at a ratio of 1:1, centrifuging at 4°C and 12 000 r / min for 30 min, taking the supernatant as 5α-reductase enzyme solution, and storing at 4°C.

[0176] The determination principle is as follows: 5α-reductase catalyzes testosterone into dihydrotestosterone in the presence of reduced coenzyme II, and the reduced coenzyme II is converted into oxidized coenzyme II, and if 5α-reductase is inhibited, the reaction process is inhibited to a certain extent. Since the reduced coenzyme II has a characteristic absorption at 340 nm wavelength, and the oxidized coenzyme II has no characteristic absorption at 340 nm wavelength. By determining the absorbance at 340 nm by ultraviolet spectrophotometer, the degree of inhibition of 5α-reductase can be reflected.

[0177] The inhibition rate test results are shown in Table 3.

[0178] Table 3: 5α-reductase inhibition rate of each sample group

[0179]

[0180]

[0181] 5α-reductase can catalyze testosterone (T) into dihydrotestosterone (DHT), which binds to androgen receptors of sebaceous gland cells to play a role to produce a large amount of sebum, thereby inducing acne.

[0182] As can be seen from the comparative data of Examples 1-10 in Table 2, the variation of the formulations of each example has little effect on the inhibition rate of 5α-reductase, which is maintained at a high level. As can be seen from Comparative Examples 1-9, the relevant variations of strains, extraction, and fermentation process in the comparative examples will lead to a decrease in the inhibition rate of 5α-reductase. As can be seen from Comparative Examples 11-12, removing lemon juice from the auxiliary material or replacing flaxseed oil with cottonseed oil will lead to a decrease in the inhibition rate of 5α-reductase, and as can be seen from Comparative Example 10, removing lemon juice from the auxiliary material and replacing flaxseed oil with cottonseed oil will not significantly decrease the inhibition rate of 5α-reductase, because vitamin C in lemon juice can effectively improve the effect of unsaturated fatty acids in flaxseed oil, but has no obvious improvement effect on cottonseed oil.

[0183] Sensory test effect experiment

[0184] (1) Preparation of samples

[0185] The Chinese medicine preparations obtained in Examples 1, 2, 6, 8, 10 and Comparative Examples 1-16 were taken as experimental groups and control groups, respectively, and a 0.5% aqueous solution of chlorhexidine acetate was taken as a competitive control group.

[0186] (2) Sensory test

[0187] The sensory test was performed on each group, and the items of the sensory test included the skin comfort after use, the improvement of acne, and the recurrence after discontinuation.

[0188] The method of the sensory test was as follows: 220 test subjects with more acne on the face were selected and randomly divided into 22 sample groups, 10 people in each group. The test method was as follows: the face was washed, and each sample group was applied on the left and right faces, and was applied for more than 2 minutes until normal absorption, 3 times a day, and was continuously applied for 15 days, then was discontinued and scored, and the recurrence was observed and scored after 30 days of discontinuation. The scoring standard was shown in Table 4, and finally the average value was obtained to obtain the sensory test score.

[0189] The sensory test results were shown in Table 5.

[0190] Table 4 Scoring standard table

[0191]

[0192] Table 5 Sensory test results of each sample group

[0193]

[0194]

[0195] From the comparison data of the experimental group, the control group and the competitive control group in Table 5, it can be seen that the Chinese medicine preparation of the embodiment is superior to Comparative Examples 1-16 and the competitive control group in the three indexes of skin comfort, improvement of acne and recurrence after discontinuation, and more than two indexes are significantly superior to Comparative Examples 1-16 and the competitive control group. From the indexes of the competitive control group, the competitive control group has a certain effect on the improvement of acne, but the score of the recurrence after discontinuation is very low, that is, it can improve acne by inhibiting bacteria, but it cannot cure acne from the root for a long time, and there is a high risk of recurrence.

[0196] From the two indicators of acne improvement and recurrence after stopping use of the comparative examples 1-9, the decrease range is similar to the decrease range of the bacteriostatic rate in Table 1 and the 5α-reductase inhibition rate in Table 3, because the decrease of the effective components will affect the above indicators. In the comparative examples 10-16, after removing or replacing the adjuvants, the three indicators are obviously decreased, especially in the skin comfort and acne improvement, and among them, when the lemon juice is removed and the linseed oil is replaced, the change of the decrease range of the three indicators is not large compared with removing the lemon juice alone or replacing the linseed oil alone, because the lemon juice mainly plays a role in improving the unsaturated fatty acids in the linseed oil; and the lecithin and cholesta-5-ene-3β-ol also have the general effect when added alone, which also proves the synergistic effect of the two; especially in the comparative example 13, when the lauryl glucoside is replaced by the glucose glycolipid, the three indicators are significantly decreased, because the lauryl glucoside is more mild to the skin than other alkyl glycoside surfactants, and has stronger penetration ability, so it has an influence on the acne improvement and recurrence.

[0197] In addition, 182 cases of experiments were carried out in the clinic, and 109 cases were basically cured, 47 cases had significant effect, 24 cases improved, and 2 cases were invalid.

[0198] The use method and course are as follows: the patient cleans the facial skin, takes the appropriate preparation, applies it on the face, focuses on applying it on the acne, applies it for more than 2 minutes until normal absorption, and then stops, 3-4 times a day, the symptoms can be obviously relieved in 5-10 days, and the patient can be basically cured in 10-20 days and no longer relapses.

[0199] In summary, the traditional Chinese medicine preparation has strong bacteriostatic ability, can significantly inhibit 5α-reductase, can rapidly relieve the acne symptoms in a short period of time, can make the acne basically cured and no longer relapse after long-term use, and according to the clinical feedback, the traditional Chinese medicine preparation has no irritation and no toxic side effects.

[0200] Although the embodiments of the present application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments without departing from the principles and purposes of the present application within the scope of the present application.

Claims

1. A traditional Chinese medicine preparation for acne treatment and skin care, characterized in that, The traditional Chinese medicine raw materials are composed of the following components by weight: Gleditsia sinensis 24-28 parts, Spermacoce latifolia 12-14 parts, Oniscus asellus 12-14 parts, raw rhubarb 12-14 parts, Phellodendron amurense 12-14 parts, Sophora flavescens 12-14 parts, Atractylodes lancea 12-14 parts, Gentiana scabra 12-14 parts, Angelica dahurica 12-14 parts, Phytolacca acinosa 12-14 parts, Kaempferia rotunda 12-14 parts, Rhus chinensis 12-14 parts, camphor 8-10 parts, borneol 1-2 parts, and licorice 1-2 parts.

2. A preparation method of a traditional Chinese medicine preparation for acne treatment and skin care, characterized in that, The method comprises the following steps: S1, traditional Chinese medicine pretreatment: taking the traditional Chinese medicine raw materials of claim 1, mixing uniformly, adding into a fermentation tank, adding 75% ethanol and stirring uniformly, stirring and treating to volatilize ethanol, and reducing the ethanol content to 50%±5% to obtain a traditional Chinese medicine pretreatment liquid; S2, microbial fermentation: adding purified water to the traditional Chinese medicine pretreatment liquid, stirring uniformly, inoculating a compound microbial starter for fermentation, adding a feeding liquid during the fermentation process, and obtaining a traditional Chinese medicine fermentation liquid after the fermentation; S3, extraction: filtering and extracting the traditional Chinese medicine fermentation liquid to obtain a filtrate and a residue; S4, post-treatment: mixing the filtrate uniformly, adding excipients and stirring uniformly, filling into bottles to obtain the acne-removing and skin-protecting traditional Chinese medicine preparation.

3. The preparation method of claim 2, characterized in that, The mass ratio of the traditional Chinese medicine raw materials to 75% ethanol in S1 is 1:(1-1.1).

4. The preparation method of claim 2, characterized in that, The stirring speed in S1 is 40-50 rpm, and the stirring time is 23-24 h.

5. The preparation method of claim 2, characterized in that, The mass ratio of the traditional Chinese medicine pretreatment liquid to purified water in S2 is 1:(9-9.5).

6. The preparation method of claim 2, characterized in that, The compound microbial starter in S2 comprises Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti.

7. The preparation method of claim 6, characterized in that, The inoculation amount of Saccharomyces cerevisiae and Bacillus amyloliquefaciens is 0.8-0.84%, and the inoculation amount of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, and Acetobacter aceti is 0.4-0.42%.

8. The preparation method of claim 2, characterized in that, The fermentation time of the compound microbial starter in S2 is 70-72 h.

9. The preparation method of claim 2, characterized in that, The feeding liquid in S2 comprises the following components and mass contents: glucose 48-50%, protein peptone 4.8-5%, cellulase 0.48-0.5%, pectinase 0.48-0.5%, papain 0.95-1%, and the balance is distilled water.

10. The preparation method of the traditional Chinese medicine preparation according to claim 2, characterized in that, The feeding liquid in S2 is added by flow feeding through a feeding port.

11. The preparation method of the traditional Chinese medicine preparation according to claim 10, characterized in that, The flow feeding is performed once per hour, and the volume ratio of the feeding amount is 0.14-0.15% per time.

12. The preparation method of the traditional Chinese medicine preparation according to claim 2, characterized in that, The filtering and extracting in S3 is a multi-stage extraction method with different concentrations of ethanol.

13. The preparation method of claim 12, characterized in that, The filtering and extracting in S3 is a three-stage extraction method with different concentrations of ethanol.

14. The preparation method of claim 13, characterized in that, The three-stage extraction method with different concentrations of ethanol in S3 comprises the following steps: First, the traditional Chinese medicine fermentation liquid obtained in S2 is filtered through a defatted cotton gauze to obtain a first-pass filtrate and a first-pass residue, 95% ethanol is added to the first-pass residue, and a first-pass extraction liquid is obtained after stirring and extraction; Then, the first extraction liquid is filtered through the absorbent cotton gauze to obtain juice, the second filtration liquid and the second residue, 50% ethanol is added into the second residue, and the feeding liquid containing 4.8-5% snail proteinase is added through the feeding port for flow feeding, and the second extraction liquid is obtained after stirring and extraction. Finally, the second extraction liquid is subjected to squeezing treatment, filtered through the absorbent cotton gauze to obtain juice, and the third filtration liquid is obtained, and the third residue is discarded or reserved.

15. The preparation method of claim 14, characterized in that, The first residue is stirred with 95% ethanol according to the mass ratio of 1:(1-1.1), and the stirring extraction time is 48-50h.

16. The preparation method of claim 14, characterized in that, The second residue is stirred with 50% ethanol according to the mass ratio of 1:(0.9-1), and the stirring extraction time is 16-18h.

17. The preparation method of claim 14, characterized in that, The flow feeding mode is that the feeding is performed once every 10 minutes, and the volume of each feeding accounts for 0.1-0.12%.

18. The preparation method of claim 2, characterized in that, The auxiliary materials in S4 include the following components and weight percentage: linseed oil 1-1.2%, lecithin 1-1.2%, cholesta-5-ene-3β-ol 0.24-0.25%, lemon juice 4-4.8%, lauryl glucoside 0.48-0.5%, ethylenediaminetetraacetic acid disodium salt 0.018-0.02%, sodium dihydrogen phosphate 0.45-0.46%, and disodium hydrogen phosphate 0.04-0.05%.

19. The application of the traditional Chinese medicine preparation for acne treatment and skin care according to claim 1 or the traditional Chinese medicine preparation for acne treatment and skin care prepared by the method according to any one of claims 2-18 in the preparation of a medicine for acne treatment and skin care.

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