Compound preparation for improving rumen function of cattle and sheep and preparation method thereof

Abstract

The present application relates to a kind of compound preparation for improving the function of cattle and sheep rumen, and 100ml compound preparation is mainly made of the following weight ratio of raw materials: gentian 3-5g, giant knotweed 3-5g, cardamom 3-5g, peilan 3-5g, log 1-2g, cypress seed 3-5g, orange peel 3-5g, sea buckthorn 3-5g, carvacol 5-7g, urea phosphate 10-15g, sodium chloride 3-5g.The present application compound preparation is aimed at the characteristics of ruminant, adjusts rumen function, promotes nutrient absorption, and improves production performance;The present application compound preparation is easily absorbed after entering the body, and clinical administration is convenient, suitable for group administration, saves manpower and material resources.

Description

Technical Field

[0001] This invention belongs to the field of feed additive technology, and mainly relates to a compound preparation for improving rumen function in cattle and sheep and its preparation method. Background Technology

[0002] The rumen is the primary stomach of ruminants. It is the most powerful natural fermentation vessel known to date for breaking down fibrous materials, and ruminants primarily digest feed nutrients through microorganisms within the rumen. The rumen microbiota can also convert non-protein nitrogen (i.e., urea) into microbial protein for absorption by cattle. The state of rumen function also determines the production performance and immune status of ruminants.

[0003] Gentian root clears excess heat from the liver and gallbladder, and eliminates damp-heat in the lower burner. It is used for damp-heat jaundice, red and swollen eyes, and itchy eczema. Gentian root can reduce liver cell necrosis and promote bile secretion, thus playing a role in protecting the liver and promoting bile secretion; gentian root can also aid digestion and improve the body's immunity.

[0004] Polygonum cuspidatum is a diuretic and dampness-removing herb with the effects of relieving dampness and jaundice, clearing heat and detoxifying, dispersing blood stasis and relieving pain, and relieving cough and resolving phlegm. It is used for damp-heat jaundice, urinary tract infections, leukorrhea, rheumatic pain, carbuncles, and boils. It contains free anthraquinones, anthraquinone glycosides, stilbene glycosides, polysaccharides, amino acids, and other components, and has effects such as anti-liver damage, improving microcirculation, expectorant, antitussive, antiasthmatic, hemostatic, analgesic, antioxidant, and antimicrobial activity.

[0005] Amomum villosum is a dampness-resolving herb. It promotes qi circulation, relieves pain, strengthens the spleen, and calms the fetus. It is used for spleen and stomach qi stagnation, indigestion, abdominal distension, vomiting, cold pain, borborygmus and diarrhea, and threatened abortion. Amomum villosum contains volatile oils such as bornyl acetate, camphor, camphene, and limonene, as well as flavonoids. It has various pharmacological effects, including regulating gastrointestinal function, anti-inflammation, analgesia, and hypoglycemia.

[0006] Eupatorium fortunei is a herb that dispels dampness with its aromatic properties, invigorates the spleen and stomach, and relieves summer heat. It is used for heatstroke and loss of appetite. It contains various volatile oils, alkaloids, sterols and their esters, organic acids, and other components. It possesses various pharmacological effects, including promoting digestion, anti-inflammation, and antimicrobial activity.

[0007] Strychnos nux-vomica is cold in nature, bitter in taste, and highly toxic. It has the effects of clearing the meridians, relieving pain, dispersing nodules, and reducing swelling. It can be used to treat symptoms such as traumatic injuries, fracture swelling and pain, stubborn rheumatism, numbness and paralysis, and sore throat.

[0008] Arborvitae seed is a calming herb that enters the heart, kidney, and large intestine meridians. It nourishes the heart and calms the mind, stops sweating, and moistens the intestines. It is used for palpitations, excessive sweating due to night sweats, and constipation due to intestinal dryness. Arborvitae seed contains diterpenoids, fatty oils, volatile oils, saponins, vitamin A, and proteins, among other components.

[0009] Orange peel regulates qi, resolves phlegm, strengthens the spleen, and relieves stagnation. It is used for loss of appetite, fullness and pain in the chest and abdomen, rumbling in the abdomen, and loose or diarrheal stools.

[0010] Sea buckthorn, with a sour and astringent taste, is warm in nature. It aids digestion, relieves stagnation, eliminates phlegm and cough, and promotes blood circulation. It is used for indigestion, abdominal distension due to food stagnation, cough with excessive phlegm, and swelling and bruising from falls. Sea buckthorn contains various vitamins, fatty acids, trace elements, linoleic acid, sea buckthorn flavonoids, superoxide dismutase, and various amino acids. Sea buckthorn oil contains abundant vitamin A, vitamin E, flavonoids, and various active substances, which can enhance the body's vitality, protect and accelerate the repair of the gastric mucosa, increase the activity of intestinal bifidobacteria, and promote wound healing.

[0011] Urea phosphate, a slow-release urea in non-protein nitrogen (NPN), contains 17% nitrogen and 19.6% phosphorus. It can increase the content of acetic acid and propionic acid and the activity of dehydrogenases in the rumen of cattle and sheep, as well as promote the absorption and utilization of nitrogen, phosphorus, and calcium. Urea phosphate releases ammonia slowly in the rumen, effectively reducing the peak concentration of ammonia in the rumen and thus offering greater safety. Ruminant rumen microorganisms can utilize the ammonia released from the decomposition of urea phosphate as a nitrogen source to synthesize microbial proteins, which are then absorbed through the intestines and converted into animal protein. This not only reduces the amount of plant protein used and lowers feed costs but also helps promote the reproduction of fiber-decomposing bacteria, thereby improving the utilization rate of crude fiber.

[0012] Sodium chloride inhibits the excessive production of acid in the rumen of ruminants, creating a suitable environment for rumen microbial activity. Chlorine is the main anion in gastric juice; it combines with hydrogen ions to form hydrochloric acid, which activates pepsin and makes gastric juice acidic, thus having a bactericidal effect. When animals are deficient in sodium chloride, they exhibit stunted growth, reduced feed conversion rate, and decreased production performance in adult animals.

[0013] This invention utilizes the processing techniques of traditional Chinese medicine tinctures to enhance the efficacy of compound preparations and achieve better therapeutic effects. Simultaneously, the ethanol in the product can significantly improve rumen function in ruminants. The product's components are tailored to the characteristics of ruminants, regulating the rumen environment, improving rumen function, and enhancing feed conversion ratio. It is safe, effective, residue-free, and does not induce drug resistance. Summary of the Invention

[0014] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a compound preparation for improving rumen function in cattle and sheep. This compound preparation is tailored to the characteristics of ruminants, regulating rumen function, promoting nutrient absorption, and improving production performance.

[0015] The second objective of this invention is to provide a method for preparing the above-mentioned compound preparation for improving rumen function in cattle and sheep.

[0016] To achieve the above objectives, the present invention adopts the following technical solution:

[0017] A compound preparation for improving rumen function in cattle and sheep, wherein each 100ml of the compound preparation is mainly made from the following raw materials in the indicated weight ratios:

[0018] Gentian root 3-5g, Polygonum cuspidatum 3-5g, Amomum villosum 3-5g, Eupatorium fortunei 3-5g, Strychnos nux-vomica 1-2g, Platycladus orientalis seed 3-5g, Citrus reticulata peel 3-5g, Hippophae rhamnoides 3-5g, Carvacrol 5-7g, Urea phosphate 10-15g, Sodium chloride 3-5g.

[0019] This invention provides a method for preparing the above-mentioned compound preparation for improving rumen function in cattle and sheep, which includes the following steps:

[0020] 1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel, and sea buckthorn, crush and sieve them, add ethanol and perform ultrasonic extraction (extraction time 25-35 minutes, extraction temperature 50±5℃), take the supernatant and filter it, add carvacrol to the filtrate and dissolve it to obtain solution A;

[0021] 2) Take purified water, add urea phosphate and sodium chloride, dissolve to obtain solution B, and set aside;

[0022] 3) Mix solution A and solution B, and dilute to volume with ethanol to obtain the final product.

[0023] Specifically, in step 1), the Chinese medicinal materials gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel, and sea buckthorn can be pulverized and passed through a 20-60 mesh sieve.

[0024] Specifically, in step 1), the volume fraction of ethanol can be 60-85%. Furthermore, in step 1), the amount of ethanol used is 2-5 times the sum of the masses of the eight Chinese medicines, that is, the mass ratio of the two is 2-5:1.

[0025] Specifically, in step 3), the ethanol can be adjusted to a final ethanol content of 45-55% (volume fraction) in the product. The alcohol content can be adjusted using purified water or 95% ethanol.

[0026] This invention provides a method of using the above-mentioned compound preparation for improving rumen function in cattle and sheep, specifically: oral administration, 10-20 ml per head of cattle or sheep each time, once a day, for 5-7 consecutive days.

[0027] This invention also provides the application of the above-mentioned compound preparation in improving rumen function in cattle and sheep.

[0028] This invention also provides the application of the above-mentioned compound preparation in improving the production performance of cattle and sheep.

[0029] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0030] 1) The compound preparation of this invention has a simple application process and is suitable for production;

[0031] 2) This invention employs a preparation process for traditional Chinese medicine tinctures, wherein the ethanol used in the preparation process can significantly enhance and improve the product's efficacy;

[0032] 3) The compound preparation of this invention is tailored to the characteristics of ruminants, regulates rumen function, promotes nutrient absorption, and improves production performance;

[0033] 4) The compound preparation of the present invention is easily absorbed after entering the body, and is convenient for clinical administration, suitable for group administration, and saves manpower and resources. Detailed Implementation

[0034] The technical solution of the present invention will be further described in detail below with reference to the embodiments, but the scope of protection of the present invention is not limited thereto.

[0035] In the following embodiments, unless otherwise specified, all raw materials used are commercially available products that can be directly purchased or can be prepared using conventional techniques in the art.

[0036] Example 1. Preparation of a compound preparation for improving rumen function in cattle and sheep

[0037] A compound preparation for improving rumen function in cattle and sheep, wherein each 100ml of the compound preparation is made from the following raw materials in the indicated weight ratios:

[0038] Gentian root 3g, Polygonum cuspidatum 5g, Amomum villosum 3g, Eupatorium fortunei 5g, Strychnos nux-vomica 1g, Platycladus orientalis seed 5g, Orange peel 3g, Hippophae rhamnoides 5g, Carvacrol 5g, Urea phosphate 10g, Sodium chloride 5g.

[0039] The specific preparation method of the above-mentioned compound preparation for improving rumen function in cattle and sheep is as follows:

[0040] (1) Take gentian, Japanese knotweed, amomum villosum, eupatorium fortunei, strychnos nux-vomica, cypress seed, orange peel and sea buckthorn, crush them through a 24-mesh sieve, add 70% ethanol for ultrasonic extraction (extraction time 30 minutes, extraction temperature 50℃, the same below), pour off the supernatant and filter it with gauze. Combine the residue squeezed out from the residue with the filtrate, add carvacrol and dissolve.

[0041] (2) Take purified water, add urea phosphate and sodium chloride, and dissolve for later use;

[0042] (3) Take the solution obtained in step (2) and add it to the solution in step (1), and make up to 100 ml with ethanol.

[0043] In this preparation method: (1) 70% ethanol in step (1) is a volume fraction;

[0044] (2) In step (1), the mass ratio of 70% ethanol to the eight kinds of traditional Chinese medicine is 4:1;

[0045] (3) The alcohol content of the final product should be 45%-55% (volume fraction), and the alcohol content can be adjusted with purified water or 95% ethanol.

[0046] Example 2. Preparation of a compound preparation for improving rumen function in cattle and sheep

[0047] A compound preparation for improving rumen function in cattle and sheep, wherein each 100ml of the compound preparation is made from the following raw materials in the indicated weight ratios:

[0048] Gentian root 5g, Polygonum cuspidatum 3g, Amomum villosum 5g, Eupatorium fortunei 3g, Strychnos nux-vomica 2g, Platycladus orientalis seed 3g, Citrus reticulata peel 5g, Hippophae rhamnoides 3g, Carvacrol 7g, Urea phosphate 15g, Sodium chloride 3g.

[0049] The specific preparation method of the above-mentioned compound preparation for improving rumen function in cattle and sheep is as follows:

[0050] (1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel and sea buckthorn, crush them through a 24-mesh sieve, add 70% ethanol for ultrasonic extraction, pour off the supernatant and filter with gauze, combine the residue squeezed out from the residue with the filtrate, add carvacrol and dissolve.

[0051] (2) Take purified water, add urea phosphate and sodium chloride, and dissolve for later use;

[0052] (3) Take the solution obtained in step (2) and add it to the solution in step (1), and make up to 100 ml with ethanol.

[0053] In this preparation method: (1) 70% ethanol in step (1) is a volume fraction;

[0054] (2) In step (1), the mass ratio of 70% ethanol to the eight kinds of traditional Chinese medicine is 4:1;

[0055] (3) The alcohol content of the final product should be 45%-55% (volume fraction), and the alcohol content can be adjusted with purified water or 95% ethanol.

[0056] Example 3. Preparation of a compound preparation for improving rumen function in cattle and sheep

[0057] A compound preparation for improving rumen function in cattle and sheep, wherein each 100ml of the compound preparation is made from the following raw materials in the indicated weight ratios:

[0058] Gentian root 4g, Polygonum cuspidatum 4g, Amomum villosum 4g, Eupatorium fortunei 4g, Strychnos nux-vomica 1.5g, Platycladus orientalis seed 4g, Citrus reticulata peel 4g, Hippophae rhamnoides 4g, Carvacrol 6g, Urea phosphate 13g, Sodium chloride 4g.

[0059] The specific preparation method of the above-mentioned compound preparation for improving rumen function in cattle and sheep is as follows:

[0060] (1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel and sea buckthorn, crush them through a 24-mesh sieve, add 70% ethanol for ultrasonic extraction, pour off the supernatant and filter with gauze, combine the residue squeezed out from the residue with the filtrate, add carvacrol and dissolve.

[0061] (2) Take purified water, add urea phosphate and sodium chloride, and dissolve for later use;

[0062] (3) Take the solution obtained in step (2) and add it to the solution in step (1), and make up to 100 ml with ethanol.

[0063] In this preparation method: (1) 70% ethanol in step (1) is a volume fraction;

[0064] (2) In step (1), the mass ratio of 70% ethanol to the eight kinds of traditional Chinese medicine is 4:1;

[0065] (3) The alcohol content of the final product should be 45%-55% (volume fraction), and the alcohol content can be adjusted with purified water or 95% ethanol.

[0066] Comparative Example 1. Preparation of a reference standard for a compound preparation used to improve rumen function in cattle and sheep.

[0067] A compound preparation for improving rumen function in cattle and sheep, wherein each 100ml of the compound preparation is made from the following raw materials in the indicated weight ratios:

[0068] Gentian root 4g, Polygonum cuspidatum 4g, Amomum villosum 4g, Eupatorium fortunei 4g, Strychnos nux-vomica 1.5g, Platycladus orientalis seed 4g, Citrus reticulata peel 4g, Hippophae rhamnoides 4g, Carvacrol 6g, Urea phosphate 13g, Sodium chloride 4g.

[0069] The specific preparation method of the above-mentioned compound preparation for improving rumen function in cattle and sheep is as follows:

[0070] (1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel and sea buckthorn, crush them through a 24-mesh sieve, add purified water for ultrasonic extraction, pour off the supernatant and filter with gauze, combine the residue squeezed out from the residue with the filtrate, add carvacrol, dissolve and set aside.

[0071] (2) Take purified water, add urea phosphate and sodium chloride, and dissolve for later use;

[0072] (3) Take the solution obtained in step (2) and add it to the solution in step (1), and make up to 100 ml with purified water.

[0073] In this preparation method, the mass ratio of water to eight kinds of traditional Chinese medicine in step (1) is 4:1.

[0074] Comparative Example 2. Preparation of a reference standard for a compound preparation used to improve rumen function in cattle and sheep.

[0075] A compound preparation for improving rumen function in cattle and sheep, which is made from the following raw materials in the indicated weight ratios:

[0076] Gentian root 4g, Polygonum cuspidatum 4g, Amomum villosum 4g, Eupatorium fortunei 4g, Strychnos nux-vomica 1.5g, Platycladus orientalis seed 4g, Citrus reticulata peel 4g, Hippophae rhamnoides 4g, Carvacrol 6g, Urea phosphate 13g, Sodium chloride 4g, Anhydrous glucose 55g.

[0077] Preparation method: Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel, and sea buckthorn, crush them through a 40-mesh sieve, add carvacrol, urea phosphate, sodium chloride, and anhydrous glucose, and mix evenly to obtain the final product.

[0078] Comparative Example 3. Preparation of a reference standard for a compound preparation used to improve rumen function in cattle and sheep.

[0079] A compound preparation for improving rumen function in cattle and sheep, wherein the weight ratio of raw materials per 100ml is as follows:

[0080] Gentian 4g, Eupatorium fortunei 4g, Platycladus orientalis seed 4g, orange peel 4g, sea buckthorn 4g, carvacrol 6g, urea phosphate 13g, sodium chloride 4g.

[0081] The specific preparation method of the above-mentioned compound preparation for improving rumen function in cattle and sheep is as follows:

[0082] (1) Take gentian, agastache, cypress seed, orange peel and sea buckthorn, crush them through a 24-mesh sieve, add 70% ethanol for ultrasonic extraction, pour off the supernatant and filter with gauze, combine the residue squeezed out from the residue with the filtrate, add carvacrol and dissolve.

[0083] (2) Take purified water, add urea phosphate and sodium chloride, and dissolve for later use;

[0084] (3) Take the solution obtained in step (2) and add it to the solution in step (1), and make up to 100 ml with ethanol.

[0085] In this preparation method: (1) 70% ethanol in step (1) is a volume fraction;

[0086] (2) In step (1), the mass ratio of 70% ethanol to the five kinds of traditional Chinese medicine is 4:1;

[0087] (3) The alcohol content of the final product should be 45%-55% (volume fraction), and the alcohol content can be adjusted with purified water or 95% ethanol.

[0088] Efficacy test of the formulation of this invention on improving rumen function in cattle and sheep

[0089] Follow these steps:

[0090] (a) Drugs and reagents

[0091] Test drugs: the formulations prepared in Examples 1, 2, and 3;

[0092] Control drugs: formulations prepared in comparative examples 1, 2, and 3.

[0093] (II) Test Methods

[0094] (1) Experimental design: 60 calves with an age of about 1 month and a weight of about 50 kg were selected and randomly divided into 5 groups of 10 calves each, including 3 experimental groups and 3 control groups.

[0095] The three experimental groups were administered the drug at a level of 15 ml per cow per day;

[0096] Control group 1 was administered the drug at a level of 15 ml per cow per day;

[0097] Control group 2 was administered the drug at a level of 30g per cow per day;

[0098] Control group 3 was administered the drug at a rate of 15 ml per cow per day.

[0099] The specific method is as follows: A 30-day trial cycle is used. For the first 15 days, 3 kg of regular milk is fed daily, and for the next 15 days, 2 kg of regular milk is fed daily, divided into two feedings, supplemented with concentrate feed at the same time. Calves are fed in individual pens, and concentrate feed is provided freely. Disease prevention and management measures are the same as usual.

[0100] The composition of the concentrate is as follows (%):

[0101]

[0102] The observation and recording items mainly include: average daily feed intake, average daily weight gain, and feed conversion ratio.

[0103] Feed conversion ratio = Daily dry matter intake / Average daily weight gain.

[0104] Feed dry matter intake = daily feed DMI + daily milk DMI (where milk is calculated as 12.3% dry matter content).

[0105] DMI stands for Dry Matter Intake.

[0106] Digestion test: A 3-day total manure digestion test was conducted during the last three days of the feeding trial. The daily feed intake and uneaten feed were accurately recorded. Feed samples were collected daily during the trial period, and the feed and uneaten feed samples from the 3 days were mixed thoroughly to prepare analytical samples. All manure samples were collected from the cattle over 24 hours, and 5% of the total fresh manure weight was taken. 20 ml of 10% sulfuric acid was added to 100 g of fresh manure, and the mixture was thoroughly mixed. Finally, the 3-day manure samples were mixed together to obtain the experimental samples.

[0107] Feed samples, leftover hay samples, and manure samples dried to constant weight were crushed using a pulverizer, passed through a 40-mesh sieve, and crude protein and crude fat were detected using conventional analytical methods.

[0108] (2) Results and analysis. See Table 1.

[0109] Table 1. Comparison of average daily feed intake, average daily weight gain, and feed conversion ratio of calves in different groups.

[0110]

[0111] Table 1 shows that the average daily weight gain of experimental groups 1, 2, and 3 was 736g, which was 18.5%, 46.6%, and 21.45% higher than that of control groups 1, 2, and 3, respectively. The average feed conversion ratio of experimental groups 1, 2, and 3 was 2.07, which was 11.9%, 22.5%, and 11.6% lower than that of control groups 1, 2, and 3, respectively. This indicates that when the compound preparation is extracted with purified water or directly mixed and fed, the effect is weaker than that of the experimental group extracted with ethanol; and the traditional Chinese medicine formulation in the invented preparation is rationally and effectively combined.

[0112] Table 2 Comparison of crude protein and crude fat digestibility among different groups

[0113]

[0114] As shown in Table 2, the average digestibility of crude protein in experimental groups 1, 2, and 3 was 77.74%, which was 13.75%, 28.94%, and 15.23% higher than that in control groups 1, 2, and 3, respectively. The average digestibility of crude fat in experimental groups 1, 2, and 3 was 71.93%, which was 10.36%, 13.72%, and 12.08% higher than that in control groups 1, 2, and 3, respectively. This demonstrates that the formulation of this invention, using this traditional Chinese medicine formula and extracted with ethanol, can significantly improve the digestibility of crude protein and crude fat in calves. Since the rumen is the main digestive organ for nutrients, this indicates that the formulation of this invention can significantly improve rumen function in calves.

[0115] Therefore, it can be seen that the product of this invention can significantly improve rumen function in calves, enhance production performance, and improve feed conversion rate, and ethanol plays a very important role in the formulation and process.

Claims

1. A compound preparation for improving rumen function in cattle and sheep, characterized in that, Each 100ml of the compound preparation is made from the following raw materials in the indicated weight ratios: Gentian root 3-5g, Polygonum cuspidatum 3-5g, Amomum villosum 3-5g, Eupatorium fortunei 3-5g, Strychnos nux-vomica 1-2g, Platycladus orientalis seed 3-5g, Citrus reticulata peel 3-5g, Hippophae rhamnoides 3-5g, Carvacrol 5-7g, Urea phosphate 10-15g, Sodium chloride 3-5g; The preparation method of the compound preparation for improving rumen function in cattle and sheep includes the following steps: 1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel, and sea buckthorn, crush and sieve them, add ethanol for ultrasonic extraction, take the supernatant and filter, add carvacrol to the filtrate and dissolve to obtain solution A; 2) Take purified water, add urea phosphate and sodium chloride, dissolve to obtain solution B, and set aside; 3) Mix solution A and solution B, and dilute to volume with ethanol to obtain the final product; In step 1), the volume fraction of ethanol is 60-85%.

2. The method for preparing the compound preparation for improving rumen function in cattle and sheep as described in claim 1, characterized in that, Includes the following steps: 1) Take gentian, Japanese knotweed, amomum villosum, agastache rugosa, strychnos nux-vomica, cypress seed, orange peel, and sea buckthorn, crush and sieve them, add ethanol for ultrasonic extraction, take the supernatant and filter, add carvacrol to the filtrate and dissolve to obtain solution A; 2) Take purified water, add urea phosphate and sodium chloride, dissolve to obtain solution B, and set aside; 3) Mix solution A and solution B, and dilute to volume with ethanol to obtain the final product; In step 1), the volume fraction of ethanol is 60-85%.

3. The method for preparing the compound preparation for improving rumen function in cattle and sheep as described in claim 2, characterized in that, In step 1), the powder is crushed through a 20-60 mesh sieve.

4. The method for preparing the compound preparation for improving rumen function in cattle and sheep as described in claim 2, characterized in that, In step 1), the amount of ethanol used is 2-5 times the sum of the masses of the eight Chinese medicines.

5. The method for preparing the compound preparation for improving rumen function in cattle and sheep as described in claim 2, characterized in that, In step 3), the ethanol is adjusted to a final ethanol content of 45-55% in the product.

6. The use of the compound preparation of claim 1 in the preparation of feed additives for improving rumen function in cattle and sheep.

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