A stir-type photobioreactor with built-in LED light source

Abstract

This invention discloses a stirred photobioreactor with an integrated LED light source, relating to the field of photosynthetic microbial culture technology. The reactor includes a tank with a compressed air and carbon dioxide mixture inlet on the lower wall of the tank. The mixed gas is introduced into the bottom of the tank through the inlet. The top of the tank has a test port, a liquid inlet, and an exhaust port. The test port can be used to detect pH and dissolved oxygen levels. The reactor also includes a stirring assembly, a suspended lamp holder, and an LED light. This invention achieves 360° rotation by designing a suspended, integrated six-sided LED light fixture inside the tank. o The directional lighting is achieved by using a stirring impeller between the intervals of the suspended six-sided LED lamp tube bracket to reduce the amount of CO2 overflow during the air-lift stirring process. This can achieve the purpose of thorough mixing, reducing foam, preventing cell floating and adhesion to the wall, avoiding light blockage, and improving light energy utilization efficiency.

Description

Technical Field

[0001] This invention relates to the field of photosynthetic microbial culture technology, specifically to a stirred photobioreactor with a built-in LED light source. Background Technology

[0002] Photosynthetic microorganisms can assimilate light energy and CO2 into organic matter such as sugars through photosynthesis, and then convert these organic matter into substances such as oils, proteins, and pigments, thus playing an important role in industries such as energy, food, feed, bait, cosmetics, and health products. In recent years, given that traditional open-track pools or closed glass tubes are heavily dependent on sunlight and easily affected by weather and seasonal factors, some studies have successively developed indoor closed-system photobioreactors with built-in light sources. This has freed them from the limitations of natural conditions, enabling the cultivation of photosynthetic microorganisms to shift towards a precise and controllable "industrialized" model.

[0003] Existing built-in light source bioreactors still have the following problems in use: Most built-in light source photobioreactors currently adopt a bottom-supply airlift mixing mode, which is prone to generating a large amount of foam, causing microalgae or other photosynthetic microorganisms to float to the surface with the bubbles, resulting in an "air flotation" phenomenon. A large number of cells aggregate at the top of the culture medium, making it difficult to grow. In addition, the algal solution around the tank wall is also difficult to mix due to the smaller distribution of bubbles, leading to cell adhesion and growth, which is not conducive to subsequent harvesting and cleaning, and also increases the probability of biological contamination. At the same time, the temperature control circulating cooling water coils of most built-in light source photobioreactors are located in different layers inside the tank, which blocks part of the LED light, thereby affecting the photosynthetic microbial cells from receiving effective light energy and reducing the growth rate.

[0004] Therefore, it is necessary to invent a stir-type photobioreactor with built-in LED light source to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to provide a stirred photobioreactor with an integrated LED light source, addressing the problems mentioned in the background art. Currently, most photobioreactors with integrated light sources employ a bottom-supply, air-lift mixing mode, which easily generates a large amount of foam. This causes microalgae or other photosynthetic microorganisms to float to the surface with the bubbles, resulting in an "air flotation" phenomenon. Furthermore, a large number of cells aggregate at the top of the culture medium, hindering growth. Additionally, the algal solution around the tank wall also suffers from poor mixing due to fewer bubbles, leading to cell adhesion and hindering subsequent harvesting and cleaning, while also increasing the probability of biological contamination. Moreover, in most photobioreactors with integrated light sources, the temperature-controlled circulating cooling water coils are located at different levels inside the tank, thus blocking some of the LED light and affecting the effective light energy received by photosynthetic microbial cells, reducing their growth rate.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a stirred photobioreactor with a built-in LED light source, comprising a reactor tank. The lower part of the tank wall is provided with a vent pipe for a mixture of compressed air and carbon dioxide. The mixture of compressed air and carbon dioxide is introduced into the bottom of the tank through the vent pipe. The top of the reactor tank has a test port, a liquid inlet, and an exhaust port. The test port can be used to detect pH and dissolved oxygen levels; the liquid inlet can be used to add nutrients or fresh culture medium; and the exhaust port is used to discharge gases from the reactor tank. A lifting device is installed at the top of the reactor tank cover. A control device is installed on the outside of the reactor tank. The reactor includes a flow meter, a CO2 flow meter, and a compressed air flow meter. The reactor tank has two visual observation windows on its side: the upper window is used to determine the liquid level, and the lower window is used to observe the growth of photosynthetic microorganisms. A sampling port is also provided on the side of the reactor tank for periodic sampling and analysis. A drain port is located below the sampling port. A cooling water coil is installed on the inner wall of the reactor tank, with a cooling water coil inlet and outlet sequentially located on the side wall. The circulating cooling water coil is close to the inner wall of the tank to avoid blocking sunlight, thereby improving light energy utilization efficiency. The reactor also includes:

[0007] The algae slurry collection device includes an algae slurry outlet at the bottom of the reactor tank, with a collection pipeline fixedly connected to the outside of the outlet. A screw handle is threaded into the collection pipeline, and the screw handle is threaded to the bottom of the closed collection pipeline. A sealing cap is fixedly connected to the upper end of the screw handle. Twisting the screw handle pushes the sealing cap to block the algae slurry outlet. The bottom of the collection pipeline has a residual algae slurry vent, and the side wall of the collection pipeline is connected to an algae slurry outlet connection pipe. Residual algae slurry inside the collection pipeline can be discharged through the vent, preventing it from being sealed inside the pipeline. In dark conditions, algae cells remaining in the pipeline die, and the algae slurry rots, affecting the algae cells cultured in the tank.

[0008] The stirring assembly includes a stirring motor fixedly connected to the top wall of the reactor tank, and the output shaft of the stirring motor is connected to a stirring rod by means of snap-fit ​​bolts, and a stirring impeller is fixedly connected to the lower end of the stirring rod.

[0009] A suspended lamp support is provided, wherein a frame is fixedly connected to the inner wall of the reactor tank, and the frame and the frame are fixedly connected by a hanging rod;

[0010] LED lights are fixedly installed between frame one and frame two, with the ends of the LED lights placed inside each groove on frame two.

[0011] Preferably, the drainage port consists of a lower supernatant drainage port and a lower supernatant drainage port, with a height difference between the lower supernatant drainage port and the lower supernatant drainage port. The lower supernatant drainage port and the lower supernatant drainage port can discharge culture medium according to the sedimentation height of photosynthetic microorganisms after the culture is completed.

[0012] Preferably, the sealing cap is fitted with a sealing ring around its periphery, and the sealing cap is perfectly matched with the algae liquid outlet of the tank, so that the sealing cap and the algae liquid outlet of the tank are perfectly matched without gaps, thus preventing the algae liquid from leaking out of the reactor tank.

[0013] Preferably, the frame has an internal hole aligned with the test port on the top of the reactor tank to facilitate monitoring of the cultivation process at any time.

[0014] Preferably, the second frame is composed of three ring plates, with the stirring rod passing through the central circular hole of the central ring plate. The three ring plates are concentrically distributed with equal spacing between them. The stirring impeller is located between the suspended lamp tube brackets. The stirring impeller is a hollow plate. The stirring impeller is located between the suspended lamp tube brackets for stirring, which avoids the photosynthetic microorganisms from floating, adhering to the wall, and generating foam due to the air rise process when the air is supplied from the bottom. The hollowed-out stirring impeller avoids the stirring resistance that exists in the whole plate during stirring.

[0015] Preferably, the number of LED lights is set to several, and the several LED lights are installed in a ring at equal intervals between the first frame and the second frame;

[0016] The LED lights are electrically connected to the controller. When specific photosynthetic microorganisms are being cultured, the light quality and power can be adjusted via the controller's control panel to provide specific lighting conditions to meet the needs of cell growth and product accumulation.

[0017] Preferably, the LED lamp comprises a waterproof sealing ring, a transparent glass sleeve, an LED bracket, and LED chips. The waterproof sealing ring is fixedly connected to the opening of the transparent glass sleeve, and the transparent glass sleeve internally houses the LED bracket and LED chips. The LED bracket is hexagonal, and the hexagonal LED lamp is uniformly fixed to a suspended lamp tube bracket, providing uniform and stable illumination. Furthermore, the LED chips are uniformly embedded in the hexagonal LED bracket, thus achieving 360° illumination. o The direction of the light.

[0018] The technical effects and advantages provided by the present invention in the above technical solution are as follows:

[0019] The structure uses stainless steel as its main frame, and its production technology is mature, allowing for customization by relevant manufacturers. Once the main frame is completed, other components simply need to be installed in their fixed positions, making installation convenient. Furthermore, due to its sealed structure, the probability of contamination by bacteria or algae is very low, ensuring the stability of the system's operation. In addition, casters are installed at the bottom for easy movement of the device.

[0020] This photobioreactor allows for online control of light quality, power, and temperature, as well as manual control of the flow rate of the compressed air and CO2 mixture. The mixture is introduced into the tank through a bottom ventilation pipe, making it simple and easy to operate. Multiple visual observation ports allow for real-time monitoring of cell growth, while the middle and lower supernatant discharge ports allow for selective discharge of supernatant based on cell sedimentation, reducing harvesting workload and thus lowering production costs.

[0021] The photobioreactor incorporates an internal stirring impeller, which agitates the cells between the suspended LED lamp holders. This better mixes the cells, preventing cell aeration, adhesion to the walls, and excessive foaming during the air-lift process, thus promoting cell growth. Simultaneously, the impeller's agitation disperses the rising compressed air and CO2 mixture, slowing the CO2's ascent in the culture medium and facilitating CO2 utilization by the cells, thereby improving CO2 efficiency. Furthermore, the circulating cooling water coils are positioned close to the tank wall to avoid blocking light, further enhancing light energy utilization efficiency and ensuring sufficient energy for cell growth, making it easier to obtain high-density microalgae cell cultures.

[0022] This photobioreactor can expand its culture volume by simply increasing the height and width of the reactor and adding a corresponding number of LED lights inside. In addition, compared with photobioreactors such as raceway ponds, this reactor occupies a very small area, less than one percent or even one-thousandth of the former, but the microalgae biomass yield per unit area can reach hundreds or thousands of times that of raceway ponds, significantly improving production capacity. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0024] Figure 1 This is a perspective view of the overall structure of the present invention;

[0025] Figure 2 This is a perspective view of the internal structure of the reactor tank (partially cut out) of the present invention;

[0026] Figure 3 This is an exploded view of the internal structure of the reactor tank (partially cut out) of the present invention;

[0027] Figure 4 This is a perspective view of the main structure of the suspended lamp tube bracket of the present invention;

[0028] Figure 5 This is a perspective view of the main structure of the LED lamp of the present invention;

[0029] Figure 6 This is a perspective view of the internal structure of the harvesting pipeline (partially cut out) in the harvesting device of the present invention.

[0030] Explanation of reference numerals in the attached figures:

[0031] 1. Reactor tank; 2. Test port; 3. Liquid inlet; 4. Exhaust port; 5. Lifting device; 6. Controller; 7. CO2 flow meter; 8. Compressed air flow meter; 9. Visual observation port one; 10. Visual observation port two; 11. Sampling port; 12. Drain port; 121. Lower and middle supernatant drain port; 122. Lower supernatant drain port; 13. Harvesting device; 131. Tank algae liquid outlet; 132. Harvesting pipeline; 133. Screw handle; 134. Sealing cap; 135. Residual algae liquid vent; 136. Algae 14. Liquid outlet connection pipe; 14. Stirring assembly; 141. Stirring motor; 142. Stirring rod; 143. Stirring impeller; 15. Cooling water coil; 16. Suspended lamp bracket; 161. Frame 1; 162. Frame 2; 163. Hanging rod; 164. Hole; 17. LED light; 171. Waterproof sealing ring; 172. Transparent glass sleeve; 173. LED bracket; 174. LED chip; 18. Mixed gas vent pipe interface; 19. Cooling water coil inlet interface; 20. Cooling water coil outlet interface. Detailed Implementation

[0032] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0033] This invention provides, for example Figure 1-6The illustrated photobioreactor with built-in LED light source and stirring includes a reactor tank 1. The lower part of the tank wall of reactor tank 1 has a vent pipe 18 for a mixed gas of compressed air and carbon dioxide. The mixed gas of compressed air and carbon dioxide is introduced into the bottom of the tank through the vent pipe. The top of reactor tank 1 has a test port 2, a liquid inlet 3, and an exhaust port 4. Test port 2 can be used to detect pH and dissolved oxygen levels. Liquid inlet 3 can be used to add nutrients or fresh culture medium. Exhaust port 4 is used to discharge gas from reactor tank 1. A lifting device 5 is installed at the top of the tank cover of reactor tank 1. A controller 6, a CO2 flow meter 7, and a compressed air supply are installed outside reactor tank 1. The reactor tank 1 includes a flow meter 8, and two visual observation ports 9 and 10 on its side. The upper visual observation port 9 is used to determine the liquid level, and the lower visual observation port 10 is used to observe the growth of photosynthetic microorganisms. A sampling port 11 is also provided on the side of the reactor tank 1 for periodic sampling and analysis. A drain port 12 is located below the sampling port 11. A cooling water coil 15 is installed on the inner wall of the reactor tank 1, and a cooling water coil inlet 19 and a cooling water coil outlet 20 are sequentially provided on the side wall of the reactor tank 1. The circulating cooling water coil 15 is close to the inner wall of the tank to avoid blocking sunlight, thereby improving the efficiency of light energy utilization. Other components include:

[0034] The algae slurry collection device 13 has an algae slurry outlet 131 at the bottom of the reactor tank 1, and a collection pipeline 132 is fixedly connected to the outside of the algae slurry outlet 131. A screw handle 133 is threadedly connected inside the collection pipeline 132. The screw handle 133 is threadedly connected to the bottom of the closed collection pipeline 132. A sealing cap 134 is fixedly connected to the upper end of the screw handle 133. Twisting the screw handle 133 pushes the sealing cap 134 to seal. The algae liquid outlet 131 of the tank is blocked. The bottom of the harvesting pipeline 132 is provided with a residual algae liquid vent 135, and the side wall of the harvesting pipeline 132 is connected to an algae liquid outlet connecting pipe 136. The residual algae liquid inside the harvesting pipeline 132 can be discharged through the residual algae liquid vent 135 to avoid the algae liquid inside the harvesting pipeline 132 being sealed in the pipeline. Under dark conditions, the algae cells remaining in the pipeline die, and the algae liquid in the pipeline rots, affecting the algae cells cultured in the tank.

[0035] The stirring assembly 14 has a stirring motor 141 fixedly connected to the top wall of the reactor tank 1, and the output shaft of the stirring motor 141 is connected to the stirring rod 142 by means of snap bolts, and the lower end of the stirring rod 142 is fixedly connected to the stirring impeller 143.

[0036] Suspended lamp support 16, frame 161 is fixedly connected to the inner wall of reactor tank 1, and frame 161 and frame 2 162 are fixedly connected by a hanging rod 163;

[0037] LED light 17 is fixedly installed between frame one 161 and frame two 162, and the end of LED light 17 is placed inside each groove on frame two 162.

[0038] The drain outlet 12 consists of a middle and lower supernatant drain outlet 121 and a lower supernatant drain outlet 122. There is a height difference between the middle and lower supernatant drain outlet 121 and the lower supernatant drain outlet 122. The middle and lower supernatant drain outlet 121 and the lower supernatant drain outlet 122 can discharge the culture medium according to the sedimentation height of the photosynthetic microorganisms after the culture is completed.

[0039] The sealing cover 134 is fitted with a sealing ring around its periphery, and the sealing cover 134 is perfectly matched with the algae liquid outlet 131 of the tank, so that the sealing cover 134 and the algae liquid outlet 131 of the tank are perfectly matched without gaps, thus preventing the algae liquid from leaking inside the reactor tank 1.

[0040] The frame 161 has a hole 164 inside that is aligned with the test port 2 on the top of the reactor tank 1, so as to facilitate the monitoring of the cultivation status at any time.

[0041] The frame 162 consists of three ring plates. The stirring rod 142 passes through the central hole of the central ring plate, and the three ring plates are concentrically distributed with equal spacing between them. The stirring impeller 143 is located between the suspended lamp tube brackets 16. The stirring impeller 143 is a hollow plate. The stirring impeller 143 is located between the suspended lamp tube brackets 16 for stirring. This avoids the floating, wall adhesion, and foaming of photosynthetic microorganisms caused by the gas rise process when the mixed gas of CO2 and air is supplied from the bottom. The hollowed-out stirring impeller 143 avoids the stirring resistance that exists in the whole plate during stirring.

[0042] The number of LED lights 17 is set to several, and the several LED lights 17 are equally spaced and installed in a ring between the first frame 161 and the second frame 162.

[0043] LED lamp 17 is electrically connected to controller 6. When specific photosynthetic microorganisms are cultured, the light quality and power can be adjusted through the control panel of controller 6 to provide specific lighting conditions to meet the needs of cell growth and product accumulation.

[0044] The LED light 17 consists of a waterproof sealing ring 171, a transparent glass sleeve 172, an LED bracket 173, and LED chips 174. The waterproof sealing ring 171 is fixedly connected to the opening of the transparent glass sleeve 172. The transparent glass sleeve 172 internally houses the LED bracket 173 and LED chips 174. The LED bracket 173 is hexagonal, and the hexagonal LED lights are evenly fixed to the suspended lamp tube bracket 16, providing uniform and stable illumination. Furthermore, the LED chips 174 are evenly embedded in the hexagonal LED bracket 173, thus achieving 360° illumination. o The direction of the light.

[0045] Working Principle: When using a stirred photobioreactor with a built-in LED light source, the ratio of different light qualities is first adjusted by a controller 6 located on the front of the reactor tank 1 to regulate the light quality and luminous power, thereby providing specific lighting conditions to meet the needs of cell growth and product accumulation. Hexagonal LEDs 17 are uniformly fixed on a suspended lamp support 16, providing uniform and stable lighting conditions. Furthermore, LED chips 174 are uniformly embedded in a regular hexagonal LED support 173, thus achieving 360° illumination. o The directional lighting is used to provide light energy for cell growth. A certain ratio of CO2 and compressed air mixture is introduced into the tank through CO2 flow meter 7 and compressed air flow meter 8 (the mixture is introduced into the reactor tank 1 through a pre-reserved air pipe at the bottom of the reactor tank 1). This can avoid the introduction of pure CO2 causing a sudden drop in the pH value of the culture medium, and at the same time reduce the amount of CO2 overflow during the airlift stirring process. The visual observation window 1 on the side of the reactor tank 1 is used to determine the liquid level, and the lower visual observation window 2 10 is used to observe the growth of photosynthetic microorganisms. The sampling port 11 is used to take samples at regular intervals for testing and analysis. The middle and lower supernatant drain ports 121 and lower supernatant drain ports 122 can discharge the culture medium after the culture is completed according to the sedimentation height of the photosynthetic microorganisms.

[0046] The stirring motor 141 is started, which drives the stirring impeller 143 fixedly connected to the lower end of the stirring rod 142 to rotate. The stirring impeller 143 is located between the suspended LED lights 17 for stirring. The stirring process can avoid the floating, wall adhesion and foaming of photosynthetic microorganisms caused by the air rise process when the bottom gas supply (a mixture of CO2 and air) is used, which is more conducive to cell growth. At the same time, the stirring of the stirring impeller 143 can disperse the rising compressed air and CO2 mixture, slowing down the rise of CO2 in the culture medium, which is more conducive to the cells to utilize CO2, thereby improving the CO2 utilization efficiency. After the stirring work is completed, the stirring motor 141 and the stirring rod 142 can be loosened and disassembled. The top cover of the reactor tank 1 can be lifted by the lifting device 5, and the top cover of the reactor tank 1 can be rotated and opened.

[0047] Meanwhile, during the use of reactor tank 1, the internal circulating cooling water coil 15 of reactor tank 1 is close to the inner wall of reactor tank 1. This avoids blocking light, improves light energy utilization efficiency, facilitates sufficient energy for cell growth, and makes it easier to obtain high-density cultured microalgae cells.

[0048] During subsequent algal slurry collection, the hose connected to the algal slurry collection tank is connected to the algal slurry outlet connection pipe 136. Turning the screw handle 133 drives the sealing cap 134 away from the algal slurry outlet 131 of the tank until it is below the algal slurry outlet connection pipe 136. With the assistance of the hose, the algal slurry can be transported into the algal slurry collection tank. After collection, the screw handle 133 is turned again to push the sealing cap 134 to seal the algal slurry outlet 131 of the tank. The algal slurry remaining in the collection pipeline 132 is vented through the residual algal slurry vent 135. This avoids the situation where, after the traditional collection valve is closed, algal slurry remains in the pipeline. If the algal slurry is sealed in the pipeline, the algal cells remaining in the pipeline will die under dark conditions, and the algal slurry in the pipeline will rot, affecting the algal cells cultured in the tank.

[0049] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A stirred photobioreactor with built-in LED light source, comprising a reactor tank (1), characterized in that, The reactor tank (1) has a compressed air and carbon dioxide mixed gas inlet pipe interface (18) on the lower part of the tank wall. The reactor tank (1) has a test port (2), a liquid inlet (3) and an exhaust port (4) reserved on the top. The top of the reactor tank (1) is equipped with a lifting device (5). The reactor tank (1) is equipped with a controller (6), a CO2 flow meter (7) and a compressed air flow meter (8) on the outside. The reactor tank (1) has a visual observation port one (9) and a visual observation port two (10) on the side. The reactor tank (1) also has a sampling port (11) on the side. The sampling port (11) has a drain port (12) below it. The reactor tank (1) has a cooling water coil (15) installed on the inner wall. The reactor tank (1) has a cooling water coil inlet interface (19) and a cooling water coil outlet interface (20) arranged sequentially on the side wall. It also includes: The algal liquid collection device (13) has an algal liquid outlet (131) at the bottom of the reactor tank (1), and a collection pipeline (132) is fixedly connected to the outside of the algal liquid outlet (131). A screw handle (133) is threadedly connected inside the collection pipeline (132). The screw handle (133) is threadedly connected to the bottom of the closed collection pipeline (132). A sealing cap (134) is fixedly connected to the upper end of the screw handle (133). A residual algal liquid vent (135) is provided at the bottom of the collection pipeline (132), and an algal liquid outlet connecting pipe (136) is connected to the side wall of the collection pipeline (132). The stirring assembly (14) has a stirring motor (141) fixedly connected to the top wall of the reactor tank (1), and the output shaft of the stirring motor (141) is connected to the stirring rod (142) by means of snap bolts, and the lower end of the stirring rod (142) is fixedly connected to the stirring impeller (143). Suspended lamp tube bracket (16), the inner wall of the reactor tank (1) is fixedly connected to the frame one (161), and the frame one (161) and the frame two (162) are fixedly connected by the hanging rod (163); LED lights (17) are fixedly installed between the first frame (161) and the second frame (162), and the ends of the LED lights (17) are placed inside each groove on the second frame (162). The inner wall of the reactor tank (1) is machined with a groove corresponding to accommodate the cooling water coil (15); The frame two (162) is composed of three ring plates. The stirring rod (142) passes through the central circular hole of the central ring plate. The three ring plates are concentrically distributed and the spacing between the three ring plates is equal. The stirring impeller (143) is located between the suspended lamp tube brackets (16). The stirring impeller (143) has an "L" shaped structure. Its end away from the stirring rod (142) extends upward along the suspended lamp tube brackets (16). The stirring process can prevent photosynthetic microorganisms from floating, adhering to the wall and generating foam due to the air rise process when supplying air from the bottom, which is more conducive to cell growth. At the same time, it can disperse the rising compressed air and CO2 mixture.

2. The photobioreactor with built-in LED light source and stirring according to claim 1, characterized in that, The drain port (12) consists of a middle and lower supernatant drain port (121) and a lower supernatant drain port (122), and there is a height difference between the middle and lower supernatant drain port (121) and the lower supernatant drain port (122).

3. The photobioreactor with built-in LED light source and stirring according to claim 1, characterized in that, The sealing cap (134) is fitted with a sealing ring around its periphery, and the sealing cap (134) is perfectly matched with the algae liquid outlet (131) of the tank.

4. The photobioreactor with built-in LED light source and stirring according to claim 1, characterized in that, The frame (161) has a hole (164) inside that is aligned with the test port (2) at the top of the reactor tank (1).

5. A stirred photobioreactor with built-in LED light source according to claim 1, characterized in that, The stirring impeller (143) is a perforated plate.

6. A stirred photobioreactor with built-in LED light source according to claim 1, characterized in that, The number of LED lights (17) is set to several, and the several LED lights (17) are installed in a ring at equal intervals between the first frame (161) and the second frame (162); The LED light (17) is electrically connected via the controller (6).

7. A stirred photobioreactor with built-in LED light source according to claim 6, characterized in that, The LED lamp (17) is composed of a waterproof sealing ring (171), a transparent glass sleeve (172), an LED bracket (173), and an LED chip (174). The waterproof sealing ring (171) is fixedly connected to the opening of the transparent glass sleeve (172), and the transparent glass sleeve (172) contains the LED bracket (173) and the LED chip (174). The LED bracket (173) is hexagonal, and the LED chip (174) is evenly embedded on the regular hexagonal LED bracket (173).

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