Method and instrument for detecting toxin content in a sample based on upconversion luminescence technology

Abstract

The present invention discloses a method and an instrument for detecting the toxin content in a sample based on upconversion luminescence technology. By adding an external temperature and humidity sensor to the upconversion luminescence detector, the present invention can obtain the temperature and humidity conditions of the environment in real time, and use the environmental temperature and humidity as input conditions to add them to the algorithm to calibrate the detection result, thereby achieving the purpose of improving the detection accuracy. Compared with the prior art, the present invention can quickly and accurately determine the accurate content of different types of mycotoxins in different samples, and the accuracy of the detection result meets the technical requirements between 85% and 115%.

Description

Technical Field

[0001] The present invention relates to the field of food safety detection, and particularly to a method and an instrument for detecting the toxin content in a sample based on up-converting luminescence technology. Background Art

[0002] In optoelectronic detection instruments based on up-converting phosphor technology (UPT), such as rapid food safety detectors, by measuring, analyzing, and processing the distribution state of up-converting phosphor (UCP) particles on an immunochromatographic test strip using UCP as a marker, the content of different types of mycotoxins in different samples can be rapidly determined.

[0003] In terms of accuracy and sensitivity, existing up-converting luminescence detection technologies mainly focus on semiconductor devices and information analysis to improve accuracy and sensitivity. For example, Chinese Patent Publication No. CN 104111330A discloses an up-converting luminescence immunochromatographic analyzer and a detection method. By using a beam with uniform light intensity distribution to irradiate the test strip's detection band and quality control band simultaneously, the detection band and quality control band can obtain the same illumination conditions. At the same time, an image edge extraction algorithm is used to extract the functional band region of the test strip, and the signal within the functional band region is integrated as a parameter for calculating the result, thereby improving the accuracy of the detection result. And by controlling the temperature of the internal optical system of the up-converting luminescence immunochromatographic analyzer, the influence of temperature changes on the performance of the up-converting luminescence immunochromatographic analyzer is reduced, the stability of the detection result is improved, and the up-converting luminescence immunochromatographic analyzer is suitable for occasions with a larger ambient temperature change range. Due to the involvement of using a temperature control system, the complexity of the instrument is increased, and in order to achieve the accuracy and stability of the detection result, it needs to run for a long time after startup to make the temperature meet the requirements. This is disadvantageous for rapid detection.

[0004] The information in the background art is only for explaining the general background of the present invention and should not be regarded as an admission or any form of suggestion that this information constitutes the prior art known to those of ordinary skill in the art. Summary of the Invention

[0005] To solve at least some of the technical problems in the prior art, the present invention provides a method and an instrument for detecting the toxin content in a sample based on up-converting luminescence technology that can control environmental impacts within an acceptable range. Specifically, the present invention includes the following content.

[0006] In a first aspect of the present invention, there is provided a method for detecting the toxin content in a sample based on up-converting luminescence technology, which at least includes:

[0007] (1) Obtain the measurement value measured from the sample added with the upconversion luminescent reagent;

[0008] (2) Obtain the temperature value and / or humidity value of the environment where the sample is located;

[0009] (3) Calibrate the measurement value with the temperature value and / or the humidity value to obtain the detection result.

[0010] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the detection result is calculated by the following formula:

[0011] Detection result = Measurement value × Influence factor,

[0012] wherein, the influence factor is related to the environmental temperature, and when the environmental temperature is equal to or higher than the calibration temperature, Influence factor = POWER((1 + Temperature coefficient, (Environmental temperature - Calibration temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10);

[0013] When the environmental temperature is less than the calibration temperature, Influence factor = POWER((1 - Temperature coefficient, (Calibration temperature - Environmental temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10).

[0014] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the sample comprises food.

[0015] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the sample includes corn, grains, flour, wheat bran, soybean meal, cottonseed meal, peanut meal, corn germ meal, rapeseed meal, compound feed, concentrated feed, and their processed products or by-products.

[0016] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the upconversion luminescent reagent in step (1) includes upconversion luminescent particles and biomolecules covalently linked thereto.

[0017] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the toxin is a mycotoxin.

[0018] In some embodiments, for the method for detecting the toxin content in a sample based on the upconversion luminescence technology according to the present invention, wherein the toxin includes aflatoxin, ochratoxin, patulin, trichothecene, zearalenone, fumonisin, vomitoxin, and T-2 toxin.

[0019] In a second aspect of the present invention, there is provided an upconversion luminescence detector, which includes an environmental detection unit, an upconversion luminescence detection unit, and a data processing unit; wherein:

[0020] The environmental detection unit is configured to be able to obtain the temperature value and / or humidity value of the environment where the sample is located;

[0021] The upconversion luminescence detection unit is configured to be able to obtain the measurement value measured from the sample to which the upconversion luminescence reagent is added;

[0022] The data processing unit is configured to be able to retrieve the temperature value, the humidity value, and the measurement value, and perform an operation of calibrating the measurement value by using the temperature value and / or the humidity value through program execution to obtain a detection result;

[0023] Preferably, the environmental detection unit includes a temperature sensor and a humidity sensor; the upconversion luminescence detection unit includes an optical system, a photoelectric conversion system, and a signal processing system.

[0024] In some embodiments, for the upconversion luminescence detector according to the present invention, wherein the data processing unit is configured to be able to calibrate the measurement value by using the temperature value and / or the humidity value to obtain a detection result;

[0025] Wherein, the calibration includes performing the following formula:

[0026] Detection result = Measurement value × Influence factor,

[0027] Wherein, the influence factor is related to the environmental temperature, and when the environmental temperature is equal to or higher than the calibration temperature, the influence factor = POWER((1 + Temperature coefficient, (Environmental temperature - Calibration temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10);

[0028] When the environmental temperature is less than the calibration temperature, the influence factor = POWER((1 - Temperature coefficient, (Calibration temperature - Environmental temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10).

[0029] In a third aspect of the present invention, there is provided a storage medium that stores at least the temperature upper limit, temperature lower limit, calibration temperature, calibration humidity, temperature coefficient, humidity coefficient, and sample information required when running the following formula:

[0030] Detection result = Measurement value × Influence factor,

[0031] Among them, the influence factor is related to the ambient temperature. When the ambient temperature is equal to or higher than the calibration temperature, the influence factor = POWER((1 + temperature coefficient, (ambient temperature - calibration temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibration humidity) / 10));

[0032] When the ambient temperature is less than the calibration temperature, the influence factor = POWER((1 - temperature coefficient, (calibration temperature - ambient temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibration humidity) / 10));

[0033] Preferably, the storage medium is designed in the form of a calibration card;

[0034] Preferably, the storage medium further includes a runnable computer program, and when the computer program is executed by a computer, it realizes the operation of the above formula or realizes the method described in the first aspect of the present invention.

[0035] At present, the usage scenarios of food safety rapid detectors are mostly some testing institutions for detecting mycotoxins in farms and animal feeds. In terms of the environmental conditions for using the instrument, the testing institutions are generally harsher than clinical laboratories and are more affected by environmental temperature and humidity. The present invention can achieve rapid detection without temperature and humidity control. Compared with the prior art, the present invention can quickly and accurately determine the accurate content of different types of mycotoxins in different samples, and the accuracy of the detection results meets the technical requirements between 85% and 115%. The present invention is particularly suitable for stable and accurate detection in an environment with a temperature of 10 - 30°C and a humidity of 10% - 90%. Detailed implementation manners

[0036] Now, various exemplary implementation manners of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0037] It should be understood that the terms described in the present invention are only for describing specific implementation manners and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that the upper and lower limits of the range and each intermediate value between them are specifically disclosed. Each intermediate value within any stated value or stated range and each smaller range between any other stated value or intermediate value within the stated range are also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0038] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described in this invention, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0039] As used herein, the term "upconversion luminescence technology" refers to a technology that uses upconversion luminescent materials as labels and detects the high-energy light emitted by the materials after low-energy light excitation of the labeled samples, thereby performing substance detection.

[0040] As used herein, the term "upconversion luminescent material" refers to any material that absorbs multiple photons in the low-energy long-wavelength band and emits photons in the high-energy short-wavelength band. Upconversion luminescent materials are generally granular. Therefore, the "upconversion luminescent materials" herein are sometimes also referred to as "upconversion luminescent particles", and their average particle size is generally 0.1-10 microns, such as 0.5-8 microns, 1-6 microns, etc. According to the different matrix components of upconversion luminescent materials, they can be classified into rare earth fluorides, rare earth oxyhalides, rare earth sulfides, rare earth oxides or composite oxides. At least one or a combination of multiple types of the above can be used in this invention.

[0041] As used herein, the term "upconversion luminescent reagent" refers to a reagent containing upconversion luminescent materials or particles and biomolecules covalently linked thereto. The type of biomolecule is not limited. For example, it can be a ligand, receptor, antibody, antigen, etc. In some embodiments, the biomolecule of this invention is an antibody, which includes but is not limited to polyclonal antibody, monoclonal antibody, chimeric antibody, nanobody, humanized antibody or fully human antibody. The antibody of this invention can be a single-chain antibody.

[0042] As used herein, the term "antibody" includes modifications of antibodies, including chemical modifications and conjugates of antibodies and other materials. Among them, examples of chemical modifications include but are not limited to acetylation, acylation, ADP-ribosylation, amidation, cross-linking cyclization, disulfide bond formation, demethylation, covalent cross-linking, cysteinylation, pyroglutamation, formylation, γ-carboxylation, glycosylation, GPI anchoring, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolysis and phosphorylation, etc. Among them, examples of conjugates include but are not limited to conjugates with nano-polymeric materials, magnetic beads, etc.

[0043] As used herein, the term "sample" includes any type of sample, such as food, feed, medicine, biological materials, etc. In certain embodiments, examples of the samples of the present invention include, but are not limited to, food crops or their processed products, such as corn, grains, flour, bran, soybean meal, cottonseed meal, peanut meal, corn germ meal, rapeseed meal, compound feed, concentrated feed, and their processed products or by-products.

[0044] As used herein, the term "toxin" refers to potentially harmful and toxic components in the sample to be tested, usually produced by the metabolism of microorganisms such as fungi and bacteria. Examples thereof include, but are not limited to, aflatoxin, ochratoxin, patulin, trichothecene, zearalenone, fumonisin, vomitoxin, and T-2 toxin.

[0045] Detection method

[0046] In a first aspect of the present invention, there is provided a method for detecting the toxin content in a sample based on upconversion luminescence technology, which at least includes the following steps:

[0047] (1) Obtaining a measured value measured from a sample to which an upconversion luminescence reagent is added;

[0048] (2) Obtaining the temperature value and / or humidity value of the environment where the sample is located;

[0049] (3) Calibrating the measured value with the temperature value and / or the humidity value to obtain a detection result.

[0050] Those skilled in the art should understand that the numbers (1), (2), (3), etc. are only for the purpose of distinguishing different steps and do not imply the order of the steps. As long as the object of the present invention can be achieved, the order of the above steps is not particularly limited. In addition, two or more of the above steps can be combined and carried out simultaneously. For example, steps (1) and (2) can be carried out simultaneously, that is, the two steps can be carried out separately at the same time, or steps (1) and (2) can be combined into one step. In addition, those skilled in the art should also understand that before and after the above steps (1)-(3), or between any of these steps, other steps or operations may be included, such as further optimizing and / or improving the method described in the present invention.

[0051] Step (1) of the present invention is a step of obtaining a measured value measured from a sample to which an upconversion luminescence reagent is added. Among them, obtaining can be directly measured by an upconversion luminescence detector, or a known measured value can be called, such as retrieving an existing measured value from a memory or the like.

[0052] Step (2) of the present invention is to obtain the temperature value and / or humidity value of the environment where the sample is located. It should be noted that the temperature value and / or humidity value are the corresponding values of the environment where the sample is measured. Similarly to step (1), the temperature value and / or humidity value can be directly measured by a sensor built into the upconversion luminescence detector, or can be the measured values obtained by calling other sensors outside the upconversion luminescence detector.

[0053] Step (3) of the present invention is to use the temperature value and / or the humidity value to calibrate the measured value to obtain a detection result. The present invention avoids the deviation caused by environmental conditions during upconversion luminescence detection by calibrating the measured value.

[0054] In some embodiments, the present invention calculates the detection result using the following formula:

[0055] Detection result = Measured value × Influence factor,

[0056] where, when the environmental temperature is equal to or higher than the calibration temperature, Influence factor = POWER((1 + Temperature coefficient, (Environmental temperature - Calibration temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10);

[0057] when the environmental temperature is less than the calibration temperature, Influence factor = POWER((1 - Temperature coefficient, (Calibration temperature - Environmental temperature)) × POWER(1 + Humidity coefficient, (Environmental humidity - Calibration humidity) / 10).

[0058] The influence factor in the present invention is an inherent coefficient related to environmental temperature, toxin type, etc., and is related to a specific temperature range. For different toxins or samples, the influence factor will be different. The influence factor can be easily obtained through experience or analysis or learning of known data.

[0059] The detection result of the present invention can be directly output through a fitting algorithm associated with environmental temperature and humidity, or can be obtained by manual calculation, and is not particularly limited thereto.

[0060] Upconversion luminescence detector

[0061] In the second aspect of the present invention, an upconversion luminescence detector is provided. In addition to the upconversion luminescence detection unit of a traditional upconversion luminescence detector, the detector of the present invention further includes an environmental detection unit and a data processing unit.

[0062] The environmental detection unit of the present invention is configured to be able to obtain the temperature value and / or humidity value of the environment where the sample is located. The environmental detection unit can be built into the upconversion luminescence detection unit, or can exist independently, and is not particularly limited thereto. The environmental detection unit can include a separate temperature sensor, a separate humidity sensor, or a temperature and humidity integrated sensor.

[0063] In the present invention, the up-conversion luminescence detection unit is not particularly limited as long as it can obtain the measurement value measured from the sample added with the up-conversion luminescence reagent, and any known unit can be adopted, which generally includes an optical system, a photoelectric conversion system and a signal processing system.

[0064] In the present invention, the data processing unit is set to be able to retrieve the temperature value, the humidity value and the measurement value, and perform an operation of calibrating the measurement value by using the temperature value and / or the humidity value through program execution to obtain a detection result. The data processing unit can be a processor, which can be integrated into the processor of a traditional up-conversion luminescence detection instrument. Each module in the processor can be implemented in whole or in part by software, hardware and their combination. The above-mentioned modules can be embedded in the processor of the instrument device in the form of hardware or independent of it, or stored in the memory of the computer device in the form of software, so as to facilitate the processor to call and execute the operations corresponding to each of the above modules.

[0065] The instrument of the present invention may further include dedicated purpose removable hardware, for example, a calibration card, which generally includes a storage device, and usually stores information required for corresponding sample detection inside, such as toxin type, temperature upper limit, temperature lower limit, calibration temperature, calibration humidity, temperature coefficient, humidity coefficient, etc.

[0066] The instrument of the present invention may further include a display device, which is used to display the detection operation steps and test results, and in particular can display reminder information. For example, if the ambient temperature is higher than the temperature upper limit, a pop-up window prompts the user that the room temperature is higher than the upper limit, which may lead to inaccurate experimental results; when the ambient temperature is lower than the temperature lower limit, a pop-up window prompts the user that the room temperature is lower than the lower limit, which may lead to inaccurate experimental results.

[0067] Storage system

[0068] In a third aspect of the present invention, a storage medium is provided, which stores at least the temperature upper limit, temperature lower limit, calibration temperature, calibration humidity, temperature coefficient, humidity coefficient and sample information required when running the following formula:

[0069] Detection result = measurement value × influence factor,

[0070] wherein, the influence factor is related to the ambient temperature, and when the ambient temperature is equal to or higher than the calibration temperature, influence factor = POWER((1 + temperature coefficient, (ambient temperature - calibration temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibration humidity) / 10);

[0071] When the ambient temperature is less than the calibrated temperature, the influence factor = POWER((1 - temperature coefficient, (calibrated temperature - ambient temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibrated humidity) / 10);

[0072] Preferably, the storage medium is designed in the form of a calibration card;

[0073] Preferably, the storage medium further includes a runnable computer program, and when the computer program is executed by a computer, it implements the operations of the above formula or implements the method described in the first aspect.

[0074] The storage medium of the present invention can be a readable medium in the form of a magnetic disk, an optical disk, a volatile storage unit, such as a random access storage unit (RAM) and / or a cache storage unit, and can further include a read-only storage unit (ROM). Generally, an upconversion luminescence instrument can read the stored information in the medium, and this information is generally stored in binary form, that is, in a form interpreted as a string of 1s and 0s. Such signals can define an application program to be executed by a microprocessor or information to be processed by an application program stored on a disk.

[0075] Embodiment

[0076] I. Description of the detection instrument

[0077] An external temperature and humidity sensor is added to the hardware of the rapid food safety detector (UPT2800-S) to obtain the ambient temperature and humidity in real time.

[0078] In the algorithm built into the rapid food safety detector, the ambient temperature and humidity are added as input variables, and a mathematical model for refining the temperature and humidity influence factor is established.

[0079] Among them, the input variables for calculating the influence factor include the following: temperature upper limit, temperature lower limit, calibrated temperature, calibrated humidity, temperature coefficient, humidity coefficient, ambient temperature, ambient humidity. Among them, the temperature upper limit is fixed at 30 °C, the temperature lower limit is fixed at 15 °C, the calibrated temperature, calibrated humidity, temperature coefficient, and humidity coefficient are set with different parameters according to different test substances, and the above parameters can be obtained from the calibration card through software. The ambient temperature and ambient humidity are the ambient temperature and humidity measured in real time and are obtained from the temperature and humidity sensor.

[0080] When the ambient temperature ≥ calibrated temperature:

[0081] The influence factor = POWER((1 + temperature coefficient, (ambient temperature - calibrated temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibrated humidity) / 10).

[0082] When the ambient temperature < calibrated temperature:

[0083] Influence factor = POWER((1 - temperature coefficient, (calibration temperature - ambient temperature)) × POWER(1 + humidity coefficient, (ambient humidity - calibration humidity) / 10).

[0084] Test result = Test result × Influence factor.

[0085] When using a rapid food safety detector to detect mycotoxins, when the ambient temperature is higher than the temperature upper limit, the detector will pop up a window to prompt the user that the room temperature is higher than the upper limit, which may lead to inaccurate experimental results; when the ambient temperature is lower than the temperature lower limit, the detector will pop up a window to prompt the user that the room temperature is lower than the lower limit, which may lead to inaccurate experimental results.

[0086] II. The specific operation steps are as follows:

[0087] 1. Turn on the rapid food safety detector (model UPT2800-S).

[0088] 2. After the detector starts successfully, enter the calibration parameter interface and insert the calibration card of the test object into the instrument for calibration operation. Among them, the temperature upper limit, temperature lower limit, calibration temperature, calibration humidity, temperature coefficient, and humidity coefficient in the algorithm are obtained from the calibration card.

[0089] 3. After successful calibration, insert the reagent card of the test object into the instrument for measurement.

[0090] 4. When the ambient temperature is between the lower limit and the upper limit of the calibration card, the instrument measures normally and displays the test result; if the ambient temperature is higher than the temperature upper limit, the detector will pop up a window to prompt the user that the room temperature is higher than the upper limit, which may lead to inaccurate experimental results; when the ambient temperature is lower than the temperature lower limit, the detector will pop up a window to prompt the user that the room temperature is lower than the lower limit, which may lead to inaccurate experimental results.

[0091] The calibration temperature and calibration humidity are determined through conventional experiments, for example, through a series of experiments. These experiments are usually carried out under various temperature and humidity conditions to simulate various actual use environments that the device may encounter. Through the collection and analysis of experimental data, the parameter values at which the device performs best at specific temperatures and humidities can be determined, namely the calibration temperature and calibration humidity. The determination of the temperature coefficient and humidity coefficient also depends on a large amount of experimental data. These coefficients reflect the laws of the device's measured values changing with temperature and humidity. Through experiments, the output values of the device at different temperatures and humidities can be measured, and the corresponding coefficients can be calculated accordingly. These coefficients are crucial for subsequent calibration and compensation processes and can help correct measurement errors caused by environmental factors more accurately. Therefore, without departing from the spirit of the present invention, the calibration temperature, calibration humidity, temperature coefficient, and humidity coefficient can be obtained by those skilled in the art through conventional experiments as needed, for example, through scientific analysis and processing based on a large amount of experimental data.

[0092] Test Example

[0093] I. Test Example of Zearalenone

[0094] The information in a certain batch of calibration cards for "zearalenone" is as follows: temperature coefficient = 0.0465, humidity coefficient = 0.0301, calibration temperature = 23.67, calibration humidity = 35.51. Standard zearalenone samples with known concentrations were detected under different temperature and humidity environments. The results are shown in Table 1 below:

[0095] Table 1

[0096]

[0097] It can be seen from Table 1 above that when the ambient temperature changes, the measured value varies greatly from the true concentration. Before calibration, the accuracy exceeds 85% - 115% of the technical requirements, while after calibration, the accuracy meets the requirements of 85% - 115%.

[0098] II. Test Results of Other Toxins

[0099] The information in a certain batch of calibration cards for "vomitoxin" is as follows: temperature coefficient = 0.0801, humidity coefficient = 0.0351, calibration temperature = 22.61, calibration humidity = 45.54. Standard vomitoxin samples with known concentrations were detected under different temperature and humidity environments. The results are shown in Table 2 below:

[0100] Table 2

[0101]

[0102] Although the present invention has been described with reference to exemplary embodiments, it should be understood that the present invention is not limited to the disclosed exemplary embodiments. Without departing from the scope or spirit of the present invention, various adjustments or changes can be made to the exemplary embodiments of the present invention specification. The scope of the claims should be construed in the broadest sense to cover all modifications and equivalent structures and functions.

Claims

1. A method for detecting toxin content in a sample based on upconversion luminescence technology, characterized in that: include: (1) obtaining a measurement value obtained from a sample measurement to which an upconversion luminescent reagent is added; (2) Obtaining the temperature and / or humidity of the environment in which the sample is located, wherein the temperature of the environment is 10-30° C. and the humidity is 10%-90%; (3) using the temperature value and / or the humidity value to calibrate the measurement value to obtain a test result; The test results are calculated using the following formula: Test result = measurement value × impact factor, Wherein, the impact factor is related to the ambient temperature, and when the ambient temperature is equal to or higher than the calibration temperature, the impact factor=POWER((1+temperature coefficient, (ambient temperature-calibration temperature))×POWER(1+humidity coefficient, (ambient humidity-calibration humidity) / 10); When the ambient temperature is lower than the calibration temperature, the impact factor = POWER ((1-temperature coefficient, (calibration temperature-ambient temperature)) × POWER (1+humidity coefficient, (ambient humidity-calibration humidity) / 10).

2. The method for detecting toxin content in a sample based on upconversion luminescence technology according to claim 1, characterized in that: The sample comprises a food product.

3. The method for detecting toxin content in a sample based on upconversion luminescence technology according to claim 1, characterized in that: The upconversion luminescent reagent described in step (1) includes upconversion luminescent particles and biological molecules covalently linked thereto.

4. The method for detecting toxin content in a sample based on up-conversion luminescence technology according to claim 1, characterized in that: The toxin is a fungal toxin.

5. The method for detecting toxin content in a sample based on up-conversion luminescence technology according to claim 1, characterized in that: The toxins include aflatoxins, ochratoxins, patulin, trichothecenes, zearalenone, fumonisins, vomitoxin, and T-2 toxin.

6. An up-conversion luminescence detector, characterized in that: It includes an environment detection unit, an up-conversion luminescence detection unit and a data processing unit; wherein: The environment detection unit is configured to obtain a temperature value and / or a humidity value of an environment in which the sample is located, wherein the temperature of the environment is 10-30° C., the humidity is 10%-90%, and the sample is a sample containing toxins; The up-conversion luminescence detection unit is configured to be able to obtain a measurement value obtained from a sample to which an up-conversion luminescence reagent is added; The data processing unit is configured to retrieve the temperature value, the humidity value and the measurement value, and to perform an operation of calibrating the measurement value using the temperature value and / or the humidity value to obtain a detection result through a program, wherein the calibration includes executing the following formula: Test result = measurement value × impact factor, Wherein, the impact factor is related to the ambient temperature, and when the ambient temperature is equal to or higher than the calibration temperature, the impact factor=POWER((1+temperature coefficient, (ambient temperature-calibration temperature))×POWER(1+humidity coefficient, (ambient humidity-calibration humidity) / 10); When the ambient temperature is lower than the calibration temperature, the impact factor = POWER ((1-temperature coefficient, (calibration temperature-ambient temperature)) × POWER (1+humidity coefficient, (ambient humidity-calibration humidity) / 10).

7. The up-conversion luminescence detector according to claim 6, characterized in that: The environment detection unit includes a temperature sensor and a humidity sensor; the up-conversion luminescence detection unit includes an optical system, a photoelectric conversion system and a signal processing system.

8. A storage medium, characterized in that: It at least stores the upper temperature limit, lower temperature limit, calibration temperature, calibration humidity, temperature coefficient, humidity coefficient and sample information required for running the following formula, wherein the sample is a sample containing toxins: Test result = measurement value × impact factor, The influencing factor is related to the ambient temperature, the ambient temperature is 10-30°C, the humidity is 10%-90%, and when the ambient temperature is equal to or higher than the calibration temperature, the influencing factor = POWER ((1+temperature coefficient, (ambient temperature-calibration temperature)) × POWER (1+humidity coefficient, (ambient humidity-calibration humidity) / 10); When the ambient temperature is lower than the calibration temperature, the impact factor = POWER ((1-temperature coefficient, (calibration temperature-ambient temperature)) × POWER (1+humidity coefficient, (ambient humidity-calibration humidity) / 10).

9. The storage medium according to claim 8, characterized in that The storage medium is designed in the form of a reference card.

10. The storage medium according to claim 8, characterized in that The storage medium also includes an executable computer program, which, when executed by a computer, implements the operation of the above formula or implements the method described in any one of claims 1 to 5.

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