A preparation method of ginsenoside extract for improving mitochondrial function

Abstract

The present invention discloses a preparation method of a ginsenoside extract for improving mitochondrial function, and the specific steps are: after pre-treatment of fresh ginseng, ultrasound-assisted flash extraction is performed, after centrifugation, an extract is obtained, and after fermentation, purification is performed, and rotary evaporation is performed using a rotary evaporator to obtain a ginsenoside extract. In the present invention, through the mutual cooperation of multiple steps, the biological components in ginseng can be gradually separated and processed, and through fermentation and enzymatic treatment, ginsenoside Rd can be converted into Rd, and ginsenoside Rd can be further converted into rare saponin F2, and finally converted into Rg3 and CK, so that the obtained ginsenoside has more excellent antioxidant and antibacterial effects, so that the application value is higher, and the crude polysaccharide components contained are separated, the purity of ginsenosides in the extract is improved, so that the mitochondrial function improvement of ginsenosides is further improved.

Description

Technical Field

[0001] The invention relates to the technical field of plant component extraction, in particular to a method for preparing a ginsenoside extract for improving mitochondrial function. Background Art

[0002] Nanoplastics refer to tiny plastic particles with a diameter of less than 100 nanometers. Due to their tiny size, nanoplastics can easily pass through biological tissues and cause potential damage to cells and even organelles. Mitochondria are important organelles in cells, mainly responsible for energy production. They play a key role in maintaining the normal function and metabolic balance of cells. When nanoplastics enter the body, they may damage mitochondria through various mechanisms. Nanoplastics are not highly toxic and can enter and affect mitochondria. Therefore, they can be used as mitochondrial damage exposure substances.

[0003] Ginseng is a perennial herbaceous plant of the Araliaceae family. Modern medical research and laboratory analysis have shown that it is rich in a substance called ginsenosides. Ginsenosides are also the main active ingredient in ginseng. Ginsenosides are a sterol compound, and the content in ginseng is about 4%. The article "Effects of ginsenosides on mitochondrial function in resuscitation rats and the selection of experimental models" has disclosed that ginsenosides can improve mitochondrial function.

[0004] The invention patent with announcement number CN104189041A discloses a method for extracting ginsenosides, comprising the following steps: raw material processing; extraction solvent extraction; extract solution processing; resin adsorption refining; alkali elution; component elution; eluent decolorization; concentration and other steps; the method adopts a specific alkali elution process, and combines it with a corresponding water extraction or alcohol extraction method, so that the product yield of the prepared ginsenoside extract is greatly improved to 7-7.5%, and the content of ginsenoside (Rb1+Rb2+Rc+Rd+Re+Rg1) is ≥45%; although the ginsenoside obtained by the extraction method has a high content, during the extraction process, ginsenosides and ginseng polysaccharides will overflow from ginseng, resulting in the extracted ginsenosides being mixed with ginseng polysaccharides, resulting in poor purity of ginsenosides, thereby affecting the efficacy of ginsenosides in improving mitochondria. Summary of the invention

[0005] In view of the problems existing in the prior art, the object of the present invention is to provide a method for preparing a ginsenoside extract for improving mitochondrial function.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for preparing a ginsenoside extract for improving mitochondrial function, the specific steps of the preparation method are:

[0008] After pre-treatment of fresh ginseng, ultrasound-assisted flash extraction is performed, and after centrifugation, an extract is obtained, which is then purified after fermentation and rotary evaporated using a rotary evaporator to obtain a ginsenoside extract.

[0009] As a further preferred embodiment of the present invention, the specific operation of pre-treatment of the fresh ginseng is as follows:

[0010] 1) crushing fresh ginseng with a grinder, passing through a 60-80 mesh sieve to obtain ginseng powder, and then extracting it with an ethanol solution under ultrasonication for 5-8 times, each extraction time being 10-20 minutes, combining the extracts, filtering them, and concentrating them under reduced pressure to obtain a concentrate;

[0011] 2) The concentrate is added to petroleum ether, and ultrasonic degreasing is performed for 30-50 minutes. The ultrasonic degreasing is repeated 3-5 times, and then the aqueous layer solution is further extracted with n-butanol. After extraction, it is concentrated under reduced pressure at 40-45° C. The concentrate is filled into D-101 macroporous resin for adsorption, and then eluted with ethanol solution. The eluate is concentrated under reduced pressure to obtain an ethanol phase substance.

[0012] Furthermore, in step 1), the power of the ultrasonic action is 200-300W;

[0013] The concentration of the ethanol solution is 70-75 wt %.

[0014] Furthermore, in step 2), the solid-liquid ratio of the concentrate to petroleum ether is 1 g: (30-50) mL;

[0015] The power of the ultrasonic degreasing is 300-500W;

[0016] The concentration of the ethanol solution is 60-65 wt %.

[0017] As a further preferred embodiment of the present invention, the specific operation of the ultrasound-assisted flash extraction is as follows:

[0018] Add the ethanol phase material into purified water, and perform flash extraction 1-2 times under intermittent ultrasonic assistance. The extraction time is 100-150s, the extraction voltage is 110-130V, the interval time of ultrasonic assistance is 3-6s, and the ultrasonic assistance time is 15-30s. After the treatment is completed, centrifuge and take the supernatant, concentrate under reduced pressure and then freeze-dry to obtain the extract dry powder.

[0019] Furthermore, the mass ratio of the ethanol phase substance to purified water is 1:(10-20);

[0020] The power of the intermittent ultrasonic assistance is 150-200W.

[0021] As a further preferred embodiment of the present invention, the specific operation of purification after fermentation is as follows:

[0022] 1) Put the dry powder of the extract into a container, add pure water to the dry powder of the extract according to the material-liquid ratio of 1g: (10-15)mL, mix well to make a homogenate, put it into a high-pressure steam sterilizer for high-temperature sterilization for 30-50min, and after the sterilization is completed, cool it naturally to obtain a sterile homogenate;

[0023] 2) adding the cultured bacterial solution to the sterile homogenate at an inoculum amount of 1-2%, mixing well, and placing in a shaker for fermentation at 120-160 r / min, a temperature of 30-32° C., and a fermentation time of 4-6 days. After the fermentation is completed, adding 5-6 times the volume of water-saturated n-butanol of the sterile homogenate, standing for 12-18 hours, and then ultrasonically treating at 250-350W for 3-5 minutes, filtering and discarding the primary filtrate to obtain a refined filtrate for standby use;

[0024] 3) The refined filtrate is centrifuged at 8000-12000 r / min for 10-20 min, the upper filtrate is poured out and set aside, the bottom product is poured into a container, the enzyme complex is added, the enzyme addition amount is controlled to be 3000-3200 U / g, and the enzymatic hydrolysis material-liquid ratio is controlled to be 1 g: (24-30) mL, and the pH value is adjusted to 4.5-5.5, and ultrasonic enzymolysis is performed at 50-53° C. and 100-200 W for 2-3 h. After the enzymatic hydrolysis is completed, the enzyme complex is inactivated, and then hot water extraction is performed at 80-85° C. for 1-2 h. After centrifugation at 8000-12000 r / min for 10-20 min, alcohol precipitation and centrifugation are performed, and then the upper product and the upper filtrate are combined, and after washing and purification, rotary evaporation is performed to obtain a high-purity ginsenoside extract.

[0025] Furthermore, in step 1), the temperature of the high temperature sterilization is 121-125°C.

[0026] Furthermore, in step 2), the bacterial liquid is obtained by culturing a composite bacterial agent composed of fermented Lactobacillus, plantarum Lactobacillus and plantarum Lactococcus in a mass ratio of 1:(1-3):(0.5-0.8) through MRS broth medium.

[0027] Furthermore, in step 3), the enzyme complex is composed of cellulase and papain in a mass ratio of 1:(1-2).

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] In the present invention, fresh ginseng is firstly pre-treated, and after the fresh ginseng is crushed, it is extracted with an ethanol solution under the action of ultrasound. The cavitation effect and thermal effect generated by the ultrasound can promote the overflow of biological components in the ginseng. Then the concentrate is put into petroleum ether for degreasing treatment to remove some impurities, and elution can be performed through adsorption treatment in D-101 macroporous resin, thereby improving the purity of the biological components; and through flash extraction under the assistance of intermittent ultrasound, the saponin, polysaccharide and other components in the biological components can be slowly released under the intermittent ultrasound, and the extraction efficiency can be maximized by combining with the flash extraction; at the same time, for some saponins and polysaccharides that are difficult to release, the present invention also performs fermentation treatment, and selects fermented lactobacillus A composite bacterial agent composed of Lactobacillus plantarum and Lactococcus plantarum is cultured in MRS broth medium to obtain a bacterial liquid, which is inoculated into a sterile homogenate. Through fermentation treatment, saponins, polysaccharides and other components can be completely released, and the bacteria in the bacterial liquid can convert ginsenosides into Rd. Ginsenoside Rd can be further converted into rare saponin F2, and finally converted into Rg3 and CK, so that the obtained ginsenosides have more excellent antioxidant and antibacterial effects, thereby having higher application value. In addition, an enzyme complex composed of cellulase and papain is selected for ultrasonic enzymatic hydrolysis treatment, and after alcohol precipitation, the crude polysaccharide components contained can be separated, thereby improving the purity of ginsenosides in the extract and further enhancing its mitochondrial function.

[0030] The preparation method of the ginsenoside extract of the present invention can gradually separate and process the biological components in ginseng through the cooperation of multiple steps, and convert ginsenoside into Rd through fermentation and enzymatic hydrolysis. Ginsenoside Rd can be further converted into rare saponin F2, and finally converted into Rg3 and CK, so that the obtained ginsenoside has more excellent antioxidant and antibacterial effects, thus having higher application value. The crude polysaccharide components contained therein are separated, thereby improving the purity of ginsenosides in the extract, so that the improvement of mitochondrial function of ginsenosides is further enhanced. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] A method for preparing a ginsenoside extract for improving mitochondrial function, the specific steps of the preparation method are:

[0034] 1) crushing fresh ginseng with a grinder, passing through a 60-mesh sieve to obtain ginseng powder, and then extracting it with a 70wt% ethanol solution under 200W ultrasound for 5 times, each extraction time for 10 minutes, combining the extracts, filtering, and concentrating under reduced pressure to obtain a concentrate;

[0035] 2) adding the concentrate to petroleum ether at a solid-liquid ratio of 1 g:30 mL, ultrasonically degreasing at 300 W for 30 min, repeating ultrasonic degreasing 3 times, and then further extracting the aqueous layer solution with n-butanol, and concentrating under reduced pressure at 40° C. after extraction, filling the concentrate into D-101 macroporous resin for adsorption, and then eluting with an ethanol solution with a concentration of 60 wt%, and concentrating the eluate under reduced pressure to obtain an ethanol phase substance;

[0036] 3) adding the ethanol phase material to purified water at a mass ratio of 1:10, flash extracting once under 150W intermittent ultrasonic assistance, the extraction time is 100s, the extraction voltage is 110V, the ultrasonic assistance interval time is 3s, the ultrasonic assistance time is 15s, and after the treatment is completed, centrifuging, taking the supernatant, concentrating under reduced pressure and freeze-drying to obtain an extract powder;

[0037] 4) Put the dry powder of the extract into a container, add pure water to the dry powder of the extract according to the solid-liquid ratio of 1g:10mL, mix well to make a homogenate, put it into a high-pressure steam sterilizer, and sterilize it at high temperature at 121°C for 30 minutes. After the sterilization is completed, cool it naturally to obtain a sterile homogenate;

[0038] 5) adding the cultured bacterial solution to the sterile homogenate at an inoculum volume of 1%, mixing well, and placing in a shaker for fermentation at a rate of 120 r / min, a temperature of 30° C., and a fermentation time of 4 days. After the fermentation is completed, adding 5 times the volume of water-saturated n-butanol to the sterile homogenate, standing for 12 hours, and then ultrasonically treating at 250 W for 3 minutes, filtering and discarding the primary filtrate to obtain a refined filtrate for later use;

[0039] 6) The refined filtrate is centrifuged at 8000 r / min for 10 min, the upper filtrate is poured out for standby use, the bottom product is poured into a container, the enzyme complex is added, the enzyme addition amount is controlled to be 3000 U / g, and the enzymatic hydrolysis material-liquid ratio is controlled to be 1 g:24 mL, and the pH value is adjusted to 4.5, and ultrasonic enzymolysis is performed at 50°C for 2 h at 100 W. After the enzymatic hydrolysis is completed, the enzyme complex is inactivated, and then hot water is extracted at 80°C for 1 h. After centrifugation at 8000 r / min for 10 min, the mixture is precipitated with alcohol and centrifuged, and then the upper product is combined with the upper filtrate, and after washing and purification, rotary evaporation is performed to obtain a high-purity ginsenoside extract;

[0040] The bacterial liquid is obtained by culturing a composite bacterial agent composed of fermented lactobacillus, plantarum lactobacillus and plantarum lactococcus in a mass ratio of 1:1:0.5 through MRS broth medium;

[0041] The enzyme complex consists of cellulase and papain in a mass ratio of 1:1.

[0042] Example 2

[0043] A method for preparing a ginsenoside extract for improving mitochondrial function, the specific steps of the preparation method are:

[0044] 1) crushing fresh ginseng with a grinder, passing through a 70-mesh sieve to obtain ginseng powder, and then extracting it with a 72 wt % ethanol solution under 250 W ultrasound for 7 times, each extraction time for 15 min, combining the extracts, filtering, and concentrating under reduced pressure to obtain a concentrate;

[0045] 2) adding the concentrate to petroleum ether at a solid-liquid ratio of 1 g:40 mL, ultrasonically degreasing at 400 W for 40 min, repeating the ultrasonic degreasing 4 times, and then further extracting the aqueous layer solution with n-butanol, and concentrating under reduced pressure at 42° C. after extraction, filling the concentrate into D-101 macroporous resin for adsorption, and then eluting with an ethanol solution with a concentration of 63 wt%, and concentrating the eluate under reduced pressure to obtain an ethanol phase substance;

[0046] 3) adding the ethanol phase material to purified water at a mass ratio of 1:15, flash extracting twice under the intermittent ultrasonic assistance of 180W, the extraction time is 120s, the extraction voltage is 120V, the interval time of ultrasonic assistance is 5s, and the ultrasonic assistance time is 25s. After the treatment is completed, centrifuging is performed, and the supernatant is taken, concentrated under reduced pressure, and then freeze-dried to obtain an extract powder;

[0047] 4) Put the dry powder of the extract into a container, add pure water to the dry powder of the extract according to the solid-liquid ratio of 1g:12mL, mix well to make a homogenate, put it into a high-pressure steam sterilizer, sterilize it at 123°C for 40min, and after the sterilization is completed, cool it naturally to obtain a sterile homogenate;

[0048] 5) adding the cultured bacterial solution to the sterile homogenate at an inoculum volume of 1.5%, mixing well, and placing in a shaker for fermentation at 150 r / min, 31° C., and 5 d. After the fermentation is completed, adding 6 times the volume of water-saturated n-butanol as the sterile homogenate, standing for 15 h, and then ultrasonically treating at 300 W for 4 min, filtering and discarding the primary filtrate to obtain a refined filtrate for standby use;

[0049] 6) The refined filtrate is centrifuged at 10000 r / min for 15 min, the upper filtrate is poured out for standby use, the bottom product is poured into a container, the enzyme complex is added, the enzyme addition amount is controlled to be 3100 U / g, and the enzymatic hydrolysis material-liquid ratio is controlled to be 1 g:28 mL, and the pH value is adjusted to 5.0, and ultrasonic enzymolysis is performed at 52°C and 150W for 2.5 h. After the enzymatic hydrolysis is completed, the enzyme complex is inactivated, and then hot water extraction is performed at 82°C for 1.5 h. After centrifugation at 10000 r / min for 15 min, alcohol precipitation and centrifugation are performed, and then the upper product and the upper filtrate are combined, and after washing and purification, rotary evaporation is performed to obtain a high-purity ginsenoside extract;

[0050] The bacterial liquid is obtained by culturing a composite bacterial agent composed of fermented lactobacillus, plantarum lactobacillus and plantarum lactococcus in a mass ratio of 1:2:0.7 through MRS broth medium;

[0051] The enzyme complex is composed of cellulase and papain in a mass ratio of 1:1.5.

[0052] Example 3

[0053] A method for preparing a ginsenoside extract for improving mitochondrial function, the specific steps of the preparation method are:

[0054] 1) crushing fresh ginseng with a grinder, passing through an 80-mesh sieve to obtain ginseng powder, and then extracting it with a 75wt% ethanol solution under 300W ultrasound for 8 times, each extraction time for 20 minutes, combining the extracts, filtering, and concentrating under reduced pressure to obtain a concentrate;

[0055] 2) adding the concentrate to petroleum ether at a solid-liquid ratio of 1 g:50 mL, ultrasonically degreasing at 500 W for 50 min, repeating ultrasonic degreasing 5 times, and then further extracting the aqueous layer solution with n-butanol, and concentrating under reduced pressure at 45° C. after extraction, filling the concentrate into D-101 macroporous resin for adsorption, and then eluting with an ethanol solution with a concentration of 65 wt%, and concentrating the eluate under reduced pressure to obtain an ethanol phase substance;

[0056] 3) adding the ethanol phase material to purified water at a mass ratio of 1:20, flash extracting twice under 200W intermittent ultrasonic assistance, the extraction time is 150s, the extraction voltage is 130V, the ultrasonic assistance interval time is 6s, the ultrasonic assistance time is 30s, after the treatment is completed, centrifuging, taking the supernatant, concentrating under reduced pressure and freeze-drying to obtain an extract powder;

[0057] 4) Put the dry powder of the extract into a container, add pure water to the dry powder of the extract according to the solid-liquid ratio of 1g:15mL, mix well to make a homogenate, put it into a high-pressure steam sterilizer, sterilize it at high temperature at 125°C for 50min, and after the sterilization is completed, cool it naturally to obtain a sterile homogenate;

[0058] 5) adding the cultured bacterial solution to the sterile homogenate at an inoculum volume of 2%, mixing well, and placing in a shaker for fermentation at 160 r / min, 32° C., and 6 d. After the fermentation is completed, adding 6 times the volume of water-saturated n-butanol to the sterile homogenate, standing for 18 h, and then ultrasonically treating at 350 W for 5 min, filtering and discarding the primary filtrate to obtain a refined filtrate for standby use;

[0059] 6) The refined filtrate is centrifuged at 12000 r / min for 20 min, the upper filtrate is poured out for standby use, the bottom product is poured into a container, the enzyme complex is added, the enzyme addition amount is controlled to be 3200 U / g, and the enzymatic hydrolysis material-liquid ratio is controlled to be 1 g:30 mL, and the pH value is adjusted to 5.5, and ultrasonic enzymolysis is performed at 53°C and 200W for 3 h. After the enzymatic hydrolysis is completed, the enzyme complex is inactivated, and then hot water extraction is performed at 85°C for 2 h. After centrifugation at 12000 r / min for 20 min, alcohol precipitation and centrifugation are performed, and then the upper product and the upper filtrate are combined, and after washing and purification, rotary evaporation is performed to obtain a high-purity ginsenoside extract;

[0060] The bacterial liquid is obtained by culturing a composite bacterial agent composed of fermented lactobacillus, plantarum lactobacillus and plantarum lactococcus in a mass ratio of 1:3:0.8 through MRS broth medium;

[0061] The enzyme complex consists of cellulase and papain in a mass ratio of 1:2.

[0062] Comparative Example 1: This comparative example is basically the same as Example 1, except that the ethanol solution extraction in step 1) is omitted.

[0063] Comparative Example 2: This comparative example is substantially the same as Example 1, except that step 2) is omitted.

[0064] Comparative Example 3: This comparative example is basically the same as Example 1, except that the intermittent ultrasonic assistance in step 3) is omitted.

[0065] Comparative Example 4: This comparative example is substantially the same as Example 1, except that step 5) is omitted.

[0066] Comparative Example 5: This comparative example is basically the same as Example 1, except that the ultrasonic enzymatic hydrolysis in step 6) is omitted.

[0067] Test experiment:

[0068] For ginseng of the same batch and origin, ginsenoside extract samples were obtained by the methods disclosed in Examples 1-3 and Comparative Examples 1-5, and then the samples were tested, and the product yield and purity of the ginsenoside extract were counted and recorded. The results are shown in Table 1.

[0069] Table 1

[0070] Example 1 Example 2 Example 3 Comparative Example 1 Product yield% 8.15 8.32 8.21 7.15 purity% 97.6 98.3 98.1 85.7 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Product yield% 7.36 7.61 7.43 7.89 purity% 88.2 91.5 89.5 92.8

[0071] As can be seen from Table 1, the preparation method of the ginsenoside extract of the present invention has a high product yield and high purity, and has a good effect of improving mitochondrial function.

[0072] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A method for preparing a ginsenoside extract for improving mitochondrial function, characterized in that: The specific steps of the preparation method are: pre-treating fresh ginseng, performing ultrasound-assisted flash extraction, obtaining an extract after centrifugation, and then purifying it after fermentation, and performing rotary evaporation using a rotary evaporator to obtain a ginsenoside extract; The specific operation of purification after the fermentation is as follows: 1) Put the dry powder of the extract into a container, add pure water to the dry powder of the extract according to the material-liquid ratio of 1g: (10-15)mL, mix well to make a homogenate, put it into a high-pressure steam sterilizer for high-temperature sterilization for 30-50min, and after the sterilization is completed, cool it naturally to obtain a sterile homogenate; 2) Add the cultured bacterial solution to the sterile homogenate at an inoculation rate of 1-2%, mix thoroughly, and place in a shaker for fermentation at 120-160 r / min, 30-32°C, and 4-6 days. After the fermentation is completed, add 5-6 times the volume of water-saturated n-butanol to the sterile homogenate, let stand for 12-18 hours, and then treat with ultrasound at 250-350W for 3-5 minutes. After filtering, discard the primary filtrate to obtain the refined filtrate for later use; 3) The refined filtrate is centrifuged at 8000-12000 r / min for 10-20 min, the upper filtrate is poured out and set aside, the bottom product is poured into a container, the enzyme complex is added, the enzyme addition amount is controlled to be 3000-3200 U / g, and the enzymatic hydrolysis material-liquid ratio is controlled to be 1 g: (24-30) mL, and the pH value is adjusted to 4.5-5.5, and ultrasonic enzymolysis is performed at 50-53° C. and 100-200 W for 2-3 h. After the enzymatic hydrolysis is completed, the enzyme complex is inactivated, and then hot water is extracted at 80-85° C. for 1-2 h, and after centrifugation at 8000-12000 r / min for 10-20 min, alcohol precipitation and centrifugation are performed, and then the upper product and the upper filtrate are combined, and after washing and purification, rotary evaporation is performed to obtain a high-purity ginsenoside extract; Wherein, in step 2), the bacterial liquid is obtained by culturing a composite bacterial agent composed of fermented Lactobacillus, plantarum Lactobacillus and plantarum Lactococcus in a mass ratio of 1: (1-3): (0.5-0.8) through MRS broth medium; In step 3), the enzyme complex is composed of cellulase and papain in a mass ratio of 1:(1-2).

2. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 1, characterized in that: The specific operation of the fresh ginseng pre-treatment is as follows: 1) Grind fresh ginseng with a grinder, pass through a 60-80 mesh sieve to obtain ginseng powder, and then extract it with ethanol solution under ultrasonic action for 5-8 times, each extraction time is 10-20 minutes, combine the extracts, filter, and concentrate under reduced pressure to obtain a concentrate; 2) Add the concentrate into petroleum ether and perform ultrasonic degreasing for 30-50 minutes. Repeat the ultrasonic degreasing 3-5 times. Then further extract the aqueous layer solution with n-butanol. After extraction, concentrate under reduced pressure at 40-45°C. Fill the concentrate into D-101 macroporous resin for adsorption. Then elute with ethanol solution. Concentrate the eluate under reduced pressure to obtain ethanol phase material.

3. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 2, characterized in that: In step 1), the power of the ultrasonic action is 200-300W; the concentration of the ethanol solution is 70-75wt%.

4. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 2, characterized in that: In step 2), the solid-liquid ratio of the concentrate to petroleum ether is 1 g: (30-50) mL; the power of the ultrasonic degreasing is 300-500 W; and the concentration of the ethanol solution is 60-65 wt%.

5. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 2, characterized in that: The specific operation of the ultrasound-assisted flash extraction is as follows: adding the ethanol phase material into purified water, flash extraction 1-2 times under intermittent ultrasound assistance, the extraction time is 100-150s, the extraction voltage is 110-130V, the interval time of ultrasound assistance is 3-6s, and the ultrasound assistance time is 15-30s. After the treatment is completed, centrifugation is performed, and the supernatant is taken, and it is concentrated under reduced pressure and freeze-dried to obtain an extract dry powder.

6. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 5, characterized in that: The mass ratio of the ethanol phase substance to purified water is 1:(10-20); the power of the intermittent ultrasonic assistance is 150-200W.

7. The method for preparing a ginsenoside extract for improving mitochondrial function according to claim 1, characterized in that: In step 1), the temperature of the high temperature sterilization is 121-125°C.