Recombinant collagen gel for vaginal repair and method of making same

Abstract

The present application relates to the field of health care technology, provide a kind of for vagina repair recombination collagen gel and preparation method thereof.The recombination collagen gel for vagina repair of the present application includes by mass fraction: recombination collagen 4-7 parts, fritillary polysaccharide 5-10 parts, oligoisomaltose 6-10 parts, thickening agent 10-15 parts, humectant 15-25 parts, pH regulator 5-10 parts, preservative 5-10 parts, water 40-50 parts.Compared with prior art, the present application creatively recombination collagen, fritillary polysaccharide and oligoisomaltose are compounded as the efficacy component of gel, through the synergistic effect of the three, reach the purpose of auxiliary treatment and repair vaginitis, increase vaginal elasticity and moisturizing.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of health care technology, more particularly, to a recombinant collagen gel for vaginal repair and a preparation method thereof. BACKGROUND

[0002] The vagina is one of the important body organs of women, connecting the uterus and external genital organs, and is composed of mucosa, muscle layer and adventitia, and is rich in extensibility. The vagina plays a variety of important physiological roles in the female body, including: one of the reproductive organs: the vagina is part of the female reproductive system, which is the channel for sperm and the birth channel for the fetus from the cervix to the outside; secretion excretion: the secretion produced by the vagina helps to keep the inside of the vagina moist, prevent dryness and infection, and provide a certain degree of self-cleaning; maintain an acidic environment: the acidic environment of the vagina helps to inhibit the growth of harmful bacteria, maintain a healthy balance of flora, and thus prevent infection; sexual intercourse comfort: the elasticity and lubricity of the vagina help to provide comfort and pleasure during sexual intercourse; reproductive hormone response: the vaginal tissue is sensitive to changes in estrogen and progesterone, so it will undergo corresponding changes during the menstrual cycle and pregnancy.

[0003] In summary of the above discussion, the vagina plays an important physiological and reproductive role in the female body, and is essential for maintaining overall health and the quality of sexual life. However, as women age, the decline in female hormones, the thinning of the vaginal mucosa, and the weakening of the protective ability of the vaginal mucosa, as well as the damage to the vaginal mucosa caused by sexual life and other reasons, can cause inflammation, redness, pain and other symptoms, so timely repair and nourishment of the vaginal mucosa is necessary. SUMMARY

[0004] Therefore, the purpose of the present application is to provide a recombinant collagen gel for vaginal repair and a preparation method thereof. The present application creatively combines recombinant collagen, fritillaria polysaccharide and isomaltooligosaccharide as the functional ingredients of the gel, and through the synergistic effect of the three, achieves the purpose of auxiliary treatment and repair of vaginitis, increase of vaginal elasticity and moisturizing.

[0005] In order to achieve the above purpose, the present application adopts the following technical solutions:

[0006] A recombinant collagen gel for vaginal repair, comprising, by mass fraction: recombinant collagen 4-7 parts, fritillaria polysaccharide 5-10 parts, isomaltooligosaccharide 6-10 parts, thickening agent 10-15 parts, humectant 15-25 parts, pH adjuster 5-10 parts, preservative 5-10 parts, and water 40-50 parts.

[0007] Recombinant collagen is one of the most popular active ingredients in the beauty industry. It has multiple functions such as moisturizing, collagen replenishment, cell proliferation promotion, antioxidant, and anti-aging. Recombinant collagen has ultra-high cell adhesion activity, forming a tight and strong network structure in the vaginal mucosa tissue, effectively promoting the thickening of the vaginal mucosa epithelium, the restoration of the microvilli structure on the cell membrane surface, the reconstruction of collagen fiber structure in the vaginal tissue, and the restoration of the supporting and elastic functions of the vaginal mucosa epithelium, providing a good foundation for the restoration of damaged vaginal tissue structure to normal.

[0008] Polysaccharide from fritillaria is a polysaccharide compound extracted from fritillaria. It is composed of many sugar molecules connected by glycosidic bonds. Polysaccharide from fritillaria can be well dissolved in water to form a gel-like substance. It has certain biological activities such as immune regulation and antioxidant, which can help enhance the body's immune function and protect cells from oxidative damage. Therefore, polysaccharide from fritillaria is often used in medicine and health products as one of the active ingredients.

[0009] Isomalto-oligosaccharide (IMO) is a functional oligosaccharide. Isomaltose, panose, isomaltotriose, and isomaltotetraose are the main components of isomalto-oligosaccharide. Isomalto-oligosaccharide can promote the growth of lactobacillus-dominated probiotics, regulate vaginal flora balance, and promote the recovery of female reproductive system self-cleaning function.

[0010] Preferably, the pH of the recombinant collagen gel for vaginal repair is 6.5-7.

[0011] Preferably, the recombinant collagen includes recombinant human collagen.

[0012] Preferably, the thickening agent includes carbomer. Carbomer can form a protective gel film on the vaginal wall, physically isolating the vaginal wall from external bacteria.

[0013] Preferably, the humectant includes glycerol and / or propylene glycol. The combination of propylene glycol and glycerol in the gel has better moisturizing effect.

[0014] Preferably, the pH regulator includes acetic acid. Adjusting the pH of the gel with acetic acid maintains a weakly acidic pH, reducing irritation to the vaginal mucosa.

[0015] Preferably, the preservative includes methylparaben. Methylparaben is widely used as a preservative in cosmetics, food, and pharmaceutical preparations.

[0016] Preferably, the recombinant collagen gel for vaginal repair comprises, by weight: 5 parts recombinant collagen, 10 parts fritillaria polysaccharide, 6 parts isomaltooligosaccharide, 12 parts thickener, 20 parts humectant, 6 parts pH adjuster, 7 parts preservative, and 40 parts water.

[0017] Preferably, the recombinant collagen gel for vaginal repair comprises, by weight: 4 parts recombinant collagen, 8 parts fritillaria polysaccharide, 9 parts isomaltooligosaccharide, 12 parts thickener, 20 parts moisturizer, 6 parts pH adjuster, 7 parts preservative, and 40 parts water.

[0018] Preferably, the recombinant collagen gel for vaginal repair comprises, by weight: 7 parts recombinant collagen, 5 parts fritillaria polysaccharide, 10 parts isomaltooligosaccharide, 12 parts thickener, 20 parts moisturizer, 6 parts pH adjuster, 7 parts preservative, and 40 parts water.

[0019] The present invention also provides a method for preparing the recombinant collagen gel for vaginal repair, comprising the following steps:

[0020] (1) Mix 40-50 parts by weight of water and 10-15 parts by weight of thickener, and then mix with 5-10 parts by weight of pH adjuster;

[0021] (2) Add 6-10 parts of isomaltooligosaccharide and 5-10 parts of fritillaria polysaccharide to the system obtained in step (1), and then add 15-25 parts of humectant and 5-10 parts of preservative.

[0022] (3) Add 4 to 7 parts of recombinant collagen to the system obtained in step (1) to obtain recombinant collagen gel for vaginal repair.

[0023] In one embodiment of the present invention, the method for preparing the recombinant collagen gel for vaginal repair includes the following steps:

[0024] (1) By weight, mix 40-50 parts of water and 10-15 parts of thickener at 50 rpm for 3-4 hours, and then mix with 5-10 parts of acetic acid at 2000 rpm for 30-50 minutes.

[0025] (2) Add 6-10 parts of isomaltooligosaccharide and 5-10 parts of fritillaria polysaccharide to the system obtained in step (1), stir for 15-30 minutes, then add 15-25 parts of humectant and 5-10 parts of preservative, stir for 15-30 minutes, and then cool the system to 40°C.

[0026] (3) Add 4 to 7 parts of recombinant collagen to the system obtained in step (1), stir for 20 to 30 minutes, and obtain recombinant collagen gel for vaginal repair.

[0027] (4) Filling:

[0028] Use a gynecological gel filling machine to fill the gel prepared in step (2) into a high-density polyethylene pre-filled vaginal applicator; the filling models and specifications include 3g / applicator, 4g / applicator or 5g / applicator;

[0029] (5) Outsourcing: Print the production batch number, production date, and expiration date onto the outer carton, and then pack it;

[0030] (6) Sampling inspection, and the qualified products are put into storage.

[0031] The present invention also provides the use of the recombinant collagen gel for vaginal repair in any one of (1) to (3);

[0032] (1) Promotes collagen secretion;

[0033] (2) Inhibit inflammatory factors;

[0034] (3) Antibacterial.

[0035] Preferably, the recombinant collagen gel for vaginal repair is used to promote collagen secretion by cells; the cells include HFF-1 cells.

[0036] Preferably, the inflammatory factor includes IL-21; when the recombinant collagen gel for vaginal repair is used to inhibit the inflammatory factor, the concentration is 200-400 μg / mL.

[0037] Preferably, the antibacterial activity includes inhibition of Escherichia coli and / or Candida albicans.

[0038] This invention relates to a recombinant collagen gel for vaginal repair, comprising three active components: recombinant collagen, fritillaria polysaccharide, and isomaltooligosaccharide. The recombinant collagen exhibits extremely high cell adhesion activity, forming a tight and robust mesh-like scaffold structure in the vaginal mucosa. This effectively promotes the thickening of the vaginal mucosal epithelium, the restoration of the microvilli structure on the cell membrane surface of the vaginal mucosal epithelium, the reconstruction of collagen fiber structure in the vaginal tissue, and the restoration of the supporting and elastic functions of the vaginal mucosal epithelium, providing a good foundation for the restoration of damaged vaginal tissue structure to normal. Isomaltooligosaccharide promotes the growth of probiotics, primarily lactobacilli, regulates the vaginal flora balance, and promotes the restoration of the self-cleaning function of the female reproductive system. Carbomer forms a protective gel film on the vaginal wall, physically isolating the vaginal wall from external bacteria, thereby preventing the colonization of pathogenic microorganisms.

[0039] The recombinant collagen gel for vaginal repair of this invention forms a dense protective film on the vaginal wall during use, preventing external microorganisms from directly contacting the vaginal mucosa, thereby achieving a good vaginal protection effect that is safe and effective. Fritillaria polysaccharide and isomaltooligosaccharide have good thickening effects, and can interact with the surface functional groups of recombinant collagen to enhance the adhesion and stability of the protective film. Through the synergistic effect of the three, it achieves the purpose of adjuvant treatment and repair of vaginitis, increasing vaginal elasticity and moisturizing. Attached Figure Description

[0040] Figure 1 This is a flowchart illustrating the preparation process of the recombinant collagen gel for vaginal repair according to an embodiment of the present invention. Detailed Implementation

[0041] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0042] To further illustrate the present invention, the following embodiments are provided for detailed description. The raw materials used in the following embodiments and comparative examples of the present invention are all commercially available products, and their sources are shown in Table 1.

[0043] Table 1

[0044]

[0045] Examples 1-3

[0046] The components and contents of the recombinant collagen gels for vaginal repair in Examples 1-3 are shown in Table 2.

[0047] Table 2. Components and content of the gel (unit: parts by mass)

[0048]

[0049]

[0050] The source of recombinant human collagen is collagen stock solution T16. The 5 portions in Example 1 represent the content of recombinant human collagen in collagen stock solution T16; the 4 portions in Example 2 represent the content of recombinant human collagen in collagen stock solution T16; and the 7 portions in Example 3 represent the content of recombinant human collagen in collagen stock solution T16.

[0051] The preparation process of the above-mentioned recombinant human collagen gel for vaginal repair is as follows: Figure 1 As shown, the process includes the following steps, among which weighing, preparation, and filling are key procedures, and are carried out in a Class 100,000 cleanroom:

[0052] (1) Weighing:

[0053] According to the formula ratio in Table 2, weigh each functional ingredient and excipient according to the "Electronic Scale Operation Instruction".

[0054] (2) Preparation:

[0055] A. Measure the amount of purified water prescribed in Table 2 and add it to the emulsifying pot. Start the stirrer, set the emulsifying pot speed to 50 rpm, and set the temperature to 60℃. Add the prescribed amount of carbomer to the emulsifying pot and stir for 3 hours to allow the carbomer to swell evenly. Then add the prescribed amount of acetic acid and stir for 30 minutes to mix it evenly. Set the emulsifying pot speed to 2000 rpm and adjust the pH of the system to 6.5.

[0056] B. Add the prescribed amounts of isomaltooligosaccharide and fritillaria polysaccharide to the emulsifying pot and stir for 15 minutes until completely dissolved. Then add glycerin, propylene glycol and methylparaben in sequence and stir for 15 minutes to dissolve and mix well. Turn on the cooling water and stir to cool. When the temperature cools to 40°C, turn off the cooling water. During stirring, adjust the pH of the system to 6.5 with acetic acid.

[0057] C. Add recombinant human collagen and continue stirring for 30 minutes to obtain a uniform colorless gel system. Take samples to test the intermediates, and proceed to the filling stage after passing the test.

[0058] (3) Filling:

[0059] The gel prepared in step (2) was filled into a high-density polyethylene pre-filled vaginal applicator using a gynecological gel filling machine, and then the cap was tightened. The filling volume was determined according to the model specification of 3g / vial. No leakage occurred during this process.

[0060] (4) Outsourcing:

[0061] A. The production batch number, production date, and expiration date should be clearly and accurately printed on the outer carton, consistent with the packaging instructions.

[0062] B. Place the inner-packaged product and the folded instruction manual into the small box.

[0063] C. Pack the small boxes into the outer packaging box, pack them up, and wait for inspection.

[0064] (5) Sampling inspection, and the qualified products are put into storage.

[0065] Verification has shown that the gel prepared according to the above method and process meets all the indicators and design requirements.

[0066] Comparative Example 1

[0067] The difference between the gel in this comparative example and that in Example 1 is that the active ingredients in this comparative example only include Fritillaria polysaccharide and isomaltooligosaccharide, and the amount of recombinant human collagen missing is allocated to the corresponding components according to the ratio of Fritillaria polysaccharide and isomaltooligosaccharide.

[0068] The composition and content of other excipients, such as thickeners and humectants, remain unchanged.

[0069] Comparative Example 2

[0070] The difference between the gel in this comparative example and that in Example 1 is that the active ingredients in this comparative example only include recombinant human collagen and isomaltooligosaccharide, and the amount of fritillaria polysaccharide missing is allocated to the corresponding components according to the ratio of recombinant human collagen and isomaltooligosaccharide.

[0071] The composition and content of other excipients, such as thickeners and humectants, remain unchanged.

[0072] Comparative Example 3

[0073] The difference between the gel in this comparative example and that in Example 1 is that the active ingredients in this comparative example only include fritillaria polysaccharide and recombinant human collagen, and the amount of missing isomaltooligosaccharide is allocated to the corresponding components according to the ratio of fritillaria polysaccharide and recombinant human collagen.

[0074] The composition and content of other excipients, such as thickeners and humectants, remain unchanged.

[0075] Comparative Example 4

[0076] The difference between the gel in this comparative example and that in Example 1 is that the fritillaria polysaccharide in Example 1 is replaced with seaweed polysaccharide.

[0077] Other components and their contents remain unchanged.

[0078] Experimental Example 1

[0079] (1) HFF-1 cells were placed at 10 4 Cells were seeded per well in a 96-well plate and cultured for 24 hours. A blank control group and a sample group were set up. The test substance in the sample group was the gel prepared in the examples and comparative examples, with 6 replicates in each group. Using complete culture medium as a diluent, different concentrations of sample working solutions were prepared according to the sample test concentration table, 100 μL / well. An equal volume of complete culture medium was added to the blank group. After culturing for 24 hours, cell morphology was observed under a microscope.

[0080] (2) Take the supernatant from the well plate and detect the collagen content by ELISA. The operation steps are as follows:

[0081] A. Serial dilution of standard: The standard is serially diluted to 500, 250, 125, 62.5, and 0 ng / mL using standard and sample diluents.

[0082] B. Collect the cell culture supernatant into a sterile centrifuge tube, centrifuge, collect the supernatant, and dilute it to an appropriate concentration to use as a test sample.

[0083] C. Add 100 μL of standard working solution and test sample (diluted 1 time) to each reaction well. Each group has 2 replicates. Incubate at 37℃ for 90 min.

[0084] D. Discard the liquid, spin dry, add 100 μL of biotin-labeled antibody working solution to each reaction well, and incubate at 37°C for 60 min.

[0085] E. Discard the liquid, spin dry, add 300 μL of washing solution to each reaction well, soak for 1 min, then spin dry. Repeat 4 times.

[0086] F. Add 100 μL of HRP-labeled streptavidin working solution to each reaction well and incubate for 30 min.

[0087] Collagen content in each group was detected by ELISA. A standard curve was obtained based on the experimental results. Collagen content was inferred from the absorbance of the standard curve. The results are shown in Table 3.

[0088] Table 3

[0089] Group Collagen secretion amount (μg / ml) Blank group 2.61 Example 1 6.34 Example 2 6.87 Example 3 6.45 Comparative Example 1 4.20 Comparative Example 2 4.67 Comparative Example 3 3.91 Comparative Example 4 3.89

[0090] As can be seen from Table 3, compared with the comparative example, the gel provided in the embodiments of the present invention can significantly promote the secretion of collagen by HFF-1 cells.

[0091] Experimental Example 2

[0092] (1) Cell seeding: RAW264.7 cells were seeded at 4500 cells / well in a 96-well plate and cultured at 37°C in a 5% CO2 incubator for 24 hours.

[0093] (2) Experimental grouping: Set up a blank group and a sample group (gels prepared in the examples and comparative examples).

[0094] (3) LPS-induced inflammatory response: Dilute 1 mg / mL LPS solution (solvent is PBS buffer) to 2 μg / mL, add 50 μL / well to the well plate, and incubate at 37℃, 5% CO2 for 4 h.

[0095] (4) Dilution and addition of test samples: Using complete culture medium as diluent, prepare sample working solution (the concentration of gel in Example 1 is set to 200 μg / mL and 400 μg / mL) according to the sample test concentration gradient of 400 μg / mL, 100 μL / well, add equal amount of complete culture medium to negative control group and positive control group, and incubate in 37℃, 5% CO2 incubator for 24h, and observe cell morphology under microscope.

[0096] (5) Take the supernatant from the well plate and detect the IL-21 content by ELISA. The operation steps are as follows:

[0097] A. Calculate the required number of enzyme strips in advance, and remove the kit 30 minutes before the experiment to allow it to return to room temperature;

[0098] B. Serial dilution of standard: The standard is serially diluted to 500, 250, 125, 62.5, and 0 ng / mL using standard and sample diluents;

[0099] C. Collect the cell culture supernatant into a sterile centrifuge tube, centrifuge to obtain the supernatant, and dilute it to an appropriate concentration as a test sample;

[0100] D. Add 100 μL of standard working solution and test sample to each reaction well. Each group has 2 replicates. Incubate at 37℃ for 90 min.

[0101] E. Discard the liquid, spin dry, add 100 μL of biotin-labeled antibody working solution to each reaction well, and incubate at 37°C for 60 min.

[0102] F. Discard the liquid, spin dry, add 300μL of washing solution to each reaction well, soak for 1 min and then spin dry, repeat 4 times.

[0103] G. Add 100 μL of HRP-labeled streptavidin working solution to each reaction well and incubate at 37°C for 30 min.

[0104] H. Add 300 μL of washing solution to each reaction well, and shake off the washing solution after a 30-second interval. Repeat 4 times.

[0105] I. Add 90 μL of colorimetric reagent to each reaction well and develop the color at 37°C in the dark for about 15 minutes.

[0106] J. Add 50 μL of stop solution to each reaction well and immediately measure the OD value at a wavelength of 450 nm using an ELISA reader.

[0107] K. Calculate the standard curve regression equation (R0) based on the known concentration of the standard and the measured OD value. 2(>0.99), substitute the OD value of the sample well into the calculation of the concentration of the measured sample, and then multiply by the dilution factor to obtain the actual IL-21 concentration of the original sample. The experimental results are shown in Table 4.

[0108] Table 4

[0109] Group Concentration (μg / mL) IL-21 secretion amount (ng / mL) Blank group / 1.6 Example 1 400 0.51 Example 1 200 0.73 Example 2 400 0.60 Example 3 400 0.62 Comparative Example 1 400 1.25 Comparative Example 2 400 1.34 Comparative Example 3 400 1.19 Comparative Example 4 400 1.40

[0110] As shown in Table 4, the gel provided in the embodiments of the present invention can significantly inhibit the secretion of IL-21 by cells compared with the comparative example. Furthermore, the gel in Example 1 has a better inhibitory effect at a concentration of 400 μg / mL than at 200 μg / mL.

[0111] Experimental Example 3

[0112] Heat the agar medium, pour it into petri dishes, and allow it to solidify. Dilute Escherichia coli and Candida albicans separately to a concentration of 10⁻⁶. 5 CFU / ml, 1 ml of bacterial suspension was taken and evenly spread on the surface of a plate. Oxford cups were placed vertically on the surface of the culture medium, and gels from the examples and comparative examples were added to the Oxford cups respectively. Sterile water was used as the blank group, and 1.5 wt% phenoxyethanol solution was used as the positive group. The petri dishes were placed in a constant temperature incubator at 37°C and incubated for 18 h. Subsequently, the diameter of the inhibition zone was measured. The experimental results are shown in Table 5.

[0113] Table 5. Average inhibition zone diameter (mm) of the gels in the examples and comparative examples.

[0114]

[0115]

[0116] As shown in Table 5, the gel provided in the embodiments of the present invention has a significant antibacterial effect against Escherichia coli and Candida albicans compared with the comparative example.

[0117] Test Example 4

[0118] (1) Prepare 10-week-old female SD rats. Inject a mixture of gentamicin and penicillin into the vaginal opening of the rats using a gavage needle. After 5 consecutive days of decontamination, collect vaginal secretions for pathogen culture, and the results are negative. Subcutaneously inject estradiol to establish pseudoestrus, and subcutaneously inject hydrocortisone to induce immunosuppression. Take rats in pseudoestrus, make a circular incision in the mucosa 1 cm from the vaginal opening with a needle, and inject Candida albicans (3×10⁻⁶) into the mucosa. 8 The injection dose is 0.6 ml / kg (CFU / ml), and the vaginal opening is plugged with a gel sponge. A second injection is given every other day. On day 7 post-inoculation, vaginal secretions are collected for microscopic examination and fungal culture. A positive result on both microscopic examination and strain identification indicates successful infection.

[0119] (2) Sixty successfully infected rats were randomly divided into six groups: the gels of Example 1 and Comparative Examples 1-4, with the same mass of physiological saline as a control. The gels were injected into the vagina of the rats at a dosage of 60 mg / kg for 5 consecutive days. Vaginal secretions of the rats were examined on the 1st and 3rd days after drug withdrawal to determine whether the test was negative and to calculate the cure rate. The results are shown in Table 6.

[0120] Table 6. Cure rate of vaginitis with gel (unit: %)

[0121] Group 1 day after drug withdrawal 3 days after drug withdrawal Control group 0 0 Example 1 90 100 Comparative Example 1 20 50 Comparative Example 2 30 50 Comparative Example 3 20 40 Comparative Example 4 40 50

[0122] As shown in Table 6, the gel provided in the embodiments of the present invention can effectively treat fungal vaginitis in SD rats compared with the comparative example.

[0123] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A recombinant collagen gel for vaginal repair, characterized in that, By weight, it includes: 4-7 parts recombinant collagen, 5-10 parts fritillaria polysaccharide, 6-10 parts isomaltooligosaccharide, 10-15 parts thickener, 15-25 parts humectant, 5-10 parts pH adjuster, 5-10 parts preservative, and 40-50 parts water. The recombinant collagen includes recombinant human collagen.

2. The recombinant collagen gel for vaginal repair according to claim 1, characterized in that, The thickener includes carbomer.

3. The recombinant collagen gel for vaginal repair according to claim 1, characterized in that, The moisturizer includes glycerin and / or propylene glycol.

4. The recombinant collagen gel for vaginal repair according to claim 1, characterized in that, The pH adjuster includes acetic acid.

5. The recombinant collagen gel for vaginal repair according to claim 1, characterized in that, The preservative includes methylparaben.

6. The recombinant collagen gel for vaginal repair according to claim 1, characterized in that, By weight, it includes: 5 parts recombinant collagen, 10 parts fritillaria polysaccharide, 6 parts isomaltooligosaccharide, 12 parts thickener, 20 parts humectant, 6 parts pH adjuster, 7 parts preservative, and 40 parts water. Alternatively, by weight, the recombinant collagen gel for vaginal repair comprises: 4 parts recombinant collagen, 8 parts fritillaria polysaccharide, 9 parts isomaltooligosaccharide, 12 parts thickener, 20 parts moisturizer, 6 parts pH adjuster, 7 parts preservative, and 40 parts water. Alternatively, by weight, the recombinant collagen gel for vaginal repair comprises: 7 parts recombinant collagen, 5 parts fritillaria polysaccharide, 10 parts isomaltooligosaccharide, 12 parts thickener, 20 parts moisturizer, 6 parts pH adjuster, 7 parts preservative, and 40 parts water.

7. The method for preparing the recombinant collagen gel for vaginal repair according to any one of claims 1-6, characterized in that, Includes the following steps: (1) Mix 40-50 parts by weight of water and 10-15 parts by weight of thickener, and then mix with 5-10 parts by weight of pH adjuster; (2) Add 6-10 parts of isomaltooligosaccharide and 5-10 parts of fritillaria polysaccharide to the system obtained in step (1), and then add 15-25 parts of humectant and 5-10 parts of preservative. (3) Add 4 to 7 parts of recombinant collagen to the system obtained in step (1) to obtain recombinant collagen gel for vaginal repair.

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