Preparation process of composition containing soluble plant dietary fibers

Abstract

The invention discloses a preparation process of a composition containing soluble plant dietary fibers. The preparation process comprises the following steps: (1) obtaining raw materials: plant emulsion containing corn flour, coarse rice flour and water; (2) second-step enzymolysis treatment: S1: under the action of high-temperature alpha-amylase, carrying out first-time enzymolysis on the plant emulsion to obtain saccharified liquid; s2, under the action of cellulase and alpha-glucosidase, performing secondary enzymolysis on the saccharified liquid to obtain enzymatic hydrolysate; and (3) post-treatment: grinding and homogenizing the enzymatic hydrolysate. According to the invention, high-temperature alpha-amylase, alpha-glucosidase and cellulase are combined for use, and the composition containing isomaltooligosacharide, soluble dietary fiber and part of monosaccharide and disaccharide is synchronously generated.

Description

Technical Field

[0001] This invention relates to a preparation process of a composition containing soluble plant dietary fiber. Background Technology

[0002] Prebiotics are substances that cannot be effectively absorbed by the host but can be utilized by beneficial gut bacteria, promoting their metabolism and proliferation, thereby regulating the gut microbiota and improving host health. Isomaltooligosaccharide is a common and widely used prebiotic in food. It is difficult for the human body to digest and utilize directly, but it can be broken down and utilized by beneficial gut bacteria, promoting their proliferation; therefore, it is also known as a bifidus factor. Isomaltooligosaccharide is a class of oligosaccharides composed of 2-6 glucose residues linked by α-1,6 glycosidic bonds. Its main components include isomaltose, panose, and isomalttriose. Numerous studies have demonstrated that isomaltooligosaccharide can bring many health benefits to the human body, including: 1) promoting the growth of intestinal bifidobacteria; 2) inhibiting the reproduction and metabolism of toxic and harmful substances; 3) preventing constipation; 4) preventing diarrhea; 5) lowering serum cholesterol; 6) protecting the liver; 7) lowering blood pressure; 8) promoting the absorption of micronutrients; and 9) preventing tooth decay.

[0003] Currently, isomaltooligosaccharide (OMOS) production uses starch as raw material, with corn starch being the most common. The starch is liquefied by adding amylase after emulsification, followed by the addition of a certain amount of α-glucosidase to generate isomaltooligosaccharide, isomaltotriose, and panose. The final product is obtained through a series of processes including enzyme inactivation, purification, decolorization, filtration, desalting, and concentration. Traditional production processes using corn starch discard other components of corn, resulting in significant raw material losses. Furthermore, the use of diverse enzyme preparations and complex operations further complicate the process. Chinese patent CN105925550B describes an OOSOS production process using starch as raw material, employing starch liquefaction and simultaneous saccharification and glycoside conversion. This scheme utilizes the combined action of high-temperature α-amylase, medium-temperature α-glucosidase, β-amylase, pullulanase, and α-glucosidase, but faces technical challenges such as complex processes, long production times, low production efficiency, and difficulty in practical industrial application.

[0004] Dietary fiber is an edible carbohydrate polymer with a degree of polymerization ≥3 that cannot be digested and absorbed by the human small intestine, but is beneficial to human health. Numerous studies have demonstrated that dietary fiber intake can bring a series of health benefits, including improving gut health and regulating blood sugar, blood lipids, and insulin. Therefore, dietary fiber is an important nutrient and is increasingly being used in food products. Chinese patent CN117551711A discloses a method for preparing corn dietary fiber, which involves removing the corn husk and using the corn husk as raw material, adding cellulase and xylanase, and then proceeding through enzymatic hydrolysis, centrifugation, purification, drying, and pulverization to obtain corn dietary fiber. However, this method is complex, and using corn husk as raw material results in low raw material utilization.

[0005] Therefore, how to provide a highly efficient preparation process for a composition containing soluble dietary fiber (such as isomaltooligosaccharide) is needed. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the low production efficiency of soluble dietary fiber, especially prebiotics (such as isomaltooligosaccharide) in the prior art, and to provide a preparation process of a composition containing soluble plant dietary fiber.

[0007] The present invention provides a process for preparing a composition containing soluble plant dietary fiber. This process can use corn kernels and brown rice kernels as raw materials, and proceed through the steps of raw material pretreatment, two-step enzymatic hydrolysis, fine grinding, homogenization, filtration, high-temperature enzyme inactivation, and drying to obtain a composition rich in isomaltooligosaccharide and soluble dietary fiber.

[0008] The technical solution adopted by the present invention to achieve the above objectives is as follows.

[0009] This invention provides a process for preparing a composition containing soluble plant dietary fiber, comprising the following steps:

[0010] (1) Obtaining raw materials: plant emulsion, wherein the plant emulsion contains corn flour, brown rice flour and water;

[0011] (2) Two-step enzymatic hydrolysis:

[0012] S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion.

[0013] S2: Under conditions of pH 4.5±0.2~6.2±0.2, and with the action of cellulase and α-glucosidase with an enzyme activity concentration ≥180000U / mL, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 50±2℃~67±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours; the enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution.

[0014] (3) Post-processing: The enzymatic hydrolysate is ground and homogenized to obtain a composition containing soluble plant dietary fiber.

[0015] In some embodiments of the present invention, the insoluble dietary fiber content in the brown rice flour is 3.0 to 6.0 g / 100 g, for example 3.4 g / 100 g.

[0016] In some embodiments of the present invention, the corn flour, the brown rice flour and the water are mixed evenly to obtain the plant emulsion.

[0017] In some embodiments of the present invention, the mass ratio of the corn flour, the brown rice flour and the water is (1.25-2.25):(1.25-2.25):(5.5-6.5), for example 2:2:6.

[0018] In some embodiments of the present invention, after the corn flour, the brown rice flour and the water are mixed evenly, they are further ground by a colloid mill.

[0019] In some embodiments of the present invention, the mixture of corn flour, brown rice flour and water can be ground by a colloid mill to obtain a uniform plant emulsion, which will not separate after being left for a period of time.

[0020] In this invention, the plant emulsion is ground using a colloid mill, which can further disrupt the cell structure of corn and brown rice, causing them to release starch granules and fully emulsify them to form a uniform and stable solution, which is beneficial for subsequent enzymatic hydrolysis.

[0021] In some embodiments of the present invention, the pH of the first enzymatic hydrolysis is 6.7±0.2 to 7.0±0.2.

[0022] In some embodiments of the present invention, the first enzymatic hydrolysis process further includes 0-0.20% calcium chloride; the percentage refers to the mass percentage relative to the plant emulsion.

[0023] In some embodiments of the present invention, the content of calcium chloride is 0-0.15% or 0.15-0.20% relative to the plant emulsion.

[0024] In some embodiments of the present invention, the enzyme activity concentration of the high-temperature α-amylase is 9000–13500 U / mL.

[0025] In some embodiments of the present invention, the content of the high-temperature α-amylase is 0.03 to 0.09%, for example 0.06 to 0.09%, relative to the plant emulsion; the percentage refers to the mass percentage relative to the plant emulsion.

[0026] In some embodiments of the present invention, the high-temperature α-amylase is derived from Bacillus licheniformis.

[0027] In some embodiments of the present invention, the first enzymatic hydrolysis is carried out under the action of 0-0.20% calcium chloride and α-amylase with an enzyme activity concentration of 9000-13500 U / mL at high temperature; the percentage refers to the mass percentage relative to the plant emulsion.

[0028] In some embodiments of the present invention, the first enzymatic hydrolysis is carried out under the action of 0-0.20% calcium chloride and 0.06-0.09% high-temperature α-amylase; the percentage refers to the mass percentage relative to the plant emulsion.

[0029] In some embodiments of the present invention, the temperature of the first enzymatic hydrolysis is 85±2℃~90±2℃, for example 80±2℃, 85±2℃ or 90±2℃.

[0030] In some embodiments of the present invention, the first enzymatic hydrolysis takes 1 to 2 hours.

[0031] In some embodiments of the present invention, the pH of the second enzymatic hydrolysis is 5.0±0.2 to 6.0±0.2, for example 5.3±0.2 to 6.0±0.2, or for example 5.5±0.2 to 6.0±0.2, or even for example 5.0±0.2, 5.3±0.2, 5.5±0.2 or 6.0±0.2.

[0032] In some embodiments of the present invention, the enzyme activity concentration of the α-glucosidase is 180,000 to 720,000 U / mL, for example 180,000 to 540,000 U / mL, or 180,000 to 360,000 U / mL, or even 180,000 U / mL, 360,000 U / mL, 540,000 U / mL or 720,000 U / mL.

[0033] In some embodiments of the present invention, the content of the α-glucosidase relative to the saccharification solution is at least 0.12%, for example 0.12 to 0.48%, or for example 0.12 to 0.36%, or for example 0.12 to 0.24%, or for example 0.12%, 0.24%, or 0.36%.

[0034] In some embodiments of the present invention, the α-glucosidase is derived from Aspergillus niger.

[0035] In some embodiments of the present invention, the content of the cellulase is ≥0.05%, for example ≥0.10%, where the percentage refers to the mass percentage relative to the saccharification solution.

[0036] In some embodiments of the present invention, the second enzymatic hydrolysis is carried out under the action of α-glucosidase with an enzyme activity concentration of 180,000 to 720,000 U / mL and at least 0.05% cellulase, where the percentage refers to the mass percentage relative to the saccharification solution.

[0037] In some embodiments of the present invention, the second enzymatic hydrolysis is carried out with 0.12 to 0.48% α-glucosidase and at least 0.05% cellulase, where percentage refers to the mass percentage relative to the saccharified solution.

[0038] In some embodiments of the present invention, the temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, for example 55±2℃~60±2℃ or 60±2℃~65±2℃, or even 55±2℃, 60±2℃ or 65±2℃.

[0039] In some embodiments of the present invention, the second enzymatic hydrolysis takes 5 to 10 hours, for example, 5 to 7 hours.

[0040] In some embodiments of the present invention, the two-step enzymatic hydrolysis process does not contain β-amylase and / or pullulanase.

[0041] In some embodiments of the present invention, the average particle size of the substances in the enzymatic hydrolysate after homogenization is ≤100 μm, for example ≤60 μm, or for example 56.96 μm.

[0042] In some embodiments of the present invention, the homogenization pressure is 300 to 500 bar, for example 400 bar.

[0043] In some embodiments of the present invention, the homogenization process further includes sieving and enzyme inactivation.

[0044] In some embodiments of the present invention, the composition containing soluble plant dietary fiber includes a prebiotic selected from isomaltooligosaccharides.

[0045] In some embodiments of the present invention, the degree of polymerization of the isomaltooligosaccharide is 2 to 9.

[0046] In some embodiments of the present invention, the method for detecting the content of isomaltooligosaccharide is GB / T20881-2017.

[0047] In some embodiments of the present invention, the soluble plant dietary fiber is a carbohydrate polymer with a degree of polymerization ≥3 that cannot be digested and absorbed by the human small intestine.

[0048] In some embodiments of the present invention, the method for detecting the soluble plant dietary fiber content is GB5009.88-2023.

[0049] In some embodiments of the present invention, the isomaltooligosaccharide content in the composition containing soluble plant dietary fiber is ≥6.0g / 100g, for example 6.0 to 15.0g / 100g.

[0050] In some embodiments of the present invention, the soluble plant dietary fiber content in the composition containing soluble plant dietary fiber is ≥3.00g / 100g, for example, 3.00 to 6.00g / 100g.

[0051] In some embodiments of the present invention, the maltose content in the composition containing soluble plant dietary fiber is less than 0.5g / 100g.

[0052] In some embodiments of the present invention, the glucose content in the composition containing soluble plant dietary fiber is ≤13.5g / 100g.

[0053] In some embodiments of the present invention, the water content of the composition containing soluble plant dietary fiber is 70-80%, and the percentage refers to the weight percentage of the composition containing soluble plant dietary fiber.

[0054] The present invention also provides a composition containing soluble plant dietary fiber, which is prepared by the above-described preparation process.

[0055] The present invention also provides a composition containing soluble plant dietary fiber, wherein the composition containing soluble plant dietary fiber comprises:

[0056] The content of isomaltooligosaccharide is ≥6g / 100g, the content of soluble plant dietary fiber is ≥3.00g / 100g, and the content of maltose is less than 0.5g / 100g.

[0057] In some embodiments of the present invention, the glucose content in the prebiotic-containing composition is ≤13.5g / 100g.

[0058] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of the present invention.

[0059] The reagents and raw materials used in this invention are all commercially available.

[0060] The positive and progressive effects of this invention are as follows:

[0061] (1) This invention uses corn kernels and brown rice kernels instead of corn starch as raw materials, which improves the utilization rate of raw materials.

[0062] (2) This invention creatively combines the starch enzymatic hydrolysis step with gelatinization, which shortens the time of the entire process, reduces the number of process steps, reduces the overall production time, and improves production efficiency.

[0063] (3) The present invention creatively selects to use high-temperature α-amylase, α-glucosidase and cellulase in combination to simultaneously generate a composition containing isomaltooligosaccharide, soluble plant dietary fiber and some monosaccharides and disaccharides. The isomaltooligosaccharide content can be ≥6.0g / 100g and the soluble plant dietary fiber content can be ≥3.00g / 100g.

[0064] (4) The overall production time of the preparation process of the present invention can be controlled within 10 hours, which can improve the overall production efficiency and has high feasibility. Detailed Implementation

[0065] The present invention is further illustrated below by way of embodiments, but the invention is not limited to the scope of the embodiments described herein. Experimental methods in the following embodiments that do not specify specific conditions were performed according to conventional methods and conditions, or as selected according to the product instructions.

[0066] In this invention, the term "prebiotic" refers to a substance that is not digested and absorbed by the host's upper digestive tract and can selectively stimulate the metabolism and proliferation of beneficial bacteria (such as Bifidobacteria and Lactobacillus) in the host's gut, thereby producing health benefits for the host.

[0067] In this invention, the term "isomaltooligosaccharide" refers to a class of oligosaccharides composed of 2 to 6 glucose residues linked by α-1,6 glycosidic bonds, with main components including isomaltose, panose, and isomalttriose. The degree of polymerization of isomaltose can be 2 to 9.

[0068] In this invention, the terms "water-soluble dietary fiber," "soluble plant dietary fiber," or "soluble dietary fiber" (SDF) refer to the water-soluble portion of dietary fiber, including indigestible oligosaccharides and some polysaccharides. For example, carbohydrate polymers with a degree of polymerization (DP) ≥ 3 that cannot be digested and absorbed by the human small intestine but have health benefits.

[0069] In this invention, the term "insoluble dietary fiber" (IDF) refers to the portion of dietary fiber that is insoluble in water.

[0070] In this invention, the chemical formula of the term "glucose" is C6H. 12 O6, CAS Registry Number 50-99-7.

[0071] In this invention, the term "maltose" refers to a disaccharide composed of two glucose units linked by an α-1,4 glycosidic bond, also known as maltobiose; CAS Registry Number 6363-53-7.

[0072] In this invention, the term "limit of detection" (LOD) is the minimum threshold at which an analytical method can reliably detect a target analyte; the smaller the value, the more sensitive the method.

[0073] In this invention, the term "wet grinding," also known as wet milling, refers to a fine processing technique in which materials are pulverized, ground, and dispersed in a liquid medium (usually water or an organic solvent). It utilizes the presence of the liquid to aid grinding, ultimately obtaining a slurry or powder with fine particle size and uniform distribution.

[0074] In this invention, the term "average particle size MV" refers to the mean volume diameter.

[0075] In some embodiments of the present invention, the corn flour is obtained by crushing corn kernels.

[0076] In some embodiments of the present invention, the corn flour has a mesh size of 50-300 mesh.

[0077] In this invention, the corn kernels generally refer to fully mature corn kernels.

[0078] In some embodiments of the present invention, the brown rice flour is obtained by crushing brown rice grains.

[0079] In this invention, the brown rice grains generally refer to the product of rice after processing and dehulling, such as brown rice that meets GB / T 18810-2002.

[0080] In some embodiments of the present invention, the brown rice flour has a mesh size of 50-300 mesh.

[0081] In some embodiments of the present invention, the water may be purified water.

[0082] In this invention, the pulverization can be a conventional pulverization process in the art, such as using a universal pulverizer.

[0083] In this invention, the universal pulverizer can be a conventional universal pulverizer in the art, such as the Baijie multi-functional pulverizer.

[0084] In this invention, the pulverizing frequency of the universal pulverizer can be 40-60Hz, for example 50Hz.

[0085] In this invention, the pulverizing power of the universal pulverizer can be 1000-1500W, for example 1400W.

[0086] In some embodiments of the present invention, the pulverization process is dry pulverization.

[0087] In some embodiments of the present invention, the mass ratio of the corn flour, the brown rice flour and the water is (1.25-2.25):(1.25-2.25):(5.5-6.5), for example 2:2:6.

[0088] In this invention, the corn flour can be conventional corn flour in the art, such as corn flour that meets the requirements of the Chinese Food Composition Table.

[0089] In some embodiments of the present invention, the nutritional composition of the corn flour is shown in the table below:

[0090]

[0091]

[0092] In this invention, the brown rice flour can be conventional brown rice flour in the art, such as brown rice flour that meets GB / T 18810-2002.

[0093] In some embodiments of the present invention, the insoluble dietary fiber content in the brown rice flour is 3.0 to 6.0 g / 100 g, for example 3.4 g / 100 g.

[0094] In some embodiments of the present invention, the nutritional components of the brown rice flour are shown in the table below:

[0095] name brown rice Edible portion, % 100 Moisture content, g / 100g 13.4 Energy, kcal / 100g 348 Protein, g / 100g 7.7 Fat, g / 100g 2.7 Carbohydrates, g / 100g 75 Insoluble dietary fiber, g / 100g 3.4 Cholesterol, mg / 100g 0 Ash content, g / 100g 1.2 Total Vitamin A, μg / 100g trace amounts Carotene, μg / 100g trace amounts Retinol, μg / 100g 0 Thiamine, mg / 100g 0.38 Riboflavin, mg / 100g 0.04 .

[0096] In some embodiments of the present invention, after the corn flour, the brown rice flour and the water are mixed evenly, they are further ground by a colloid mill.

[0097] In this invention, the colloid mill can be a conventional colloid mill in the art, such as the JMS-500 Langtong colloid mill.

[0098] In this invention, the rotor speed of the colloid mill can be 2500-3000 r / min, for example 2840 r / min.

[0099] In this invention, the motor power of the colloid mill can be 1.0 to 5.0 kW, for example 1.5 kW.

[0100] In some embodiments of the present invention, the colloid mill grinding process is wet grinding.

[0101] In some embodiments of the present invention, the colloid mill may be used for grinding 3 to 5 times.

[0102] In some embodiments of the present invention, the raw material pretreatment includes the following steps:

[0103] i: Wash the corn kernels and brown rice kernels thoroughly, and then grind them separately using a grinder to obtain corn flour and brown rice flour;

[0104] ii: Prepare the emulsion by mixing corn flour, brown rice flour and water in a mass ratio of (1.25~2.25):(1.25~2.25):(5.5~6.5) and stirring until evenly mixed.

[0105] iii: The plant emulsion described in ii is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0106] In this invention, those skilled in the art can determine the pH using known methods. The pH range can fluctuate within a certain range, for example, within ±0.2.

[0107] In this invention, the pH can be adjusted using pH adjusters commonly used in the art, such as glycine-HCl or citrate buffer.

[0108] The citrate buffer solution can be prepared using citric acid or baking soda (sodium bicarbonate, NaHCO3).

[0109] In some embodiments of the present invention, the pH of the first enzymatic hydrolysis is 6.7±0.2 to 7.0±0.2.

[0110] In some embodiments of the present invention, the content of calcium chloride is 0-0.15% or 0.15-0.20% relative to the plant emulsion.

[0111] In this invention, calcium chloride can improve the activity and stability of high-temperature α-amylase.

[0112] In some embodiments of the present invention, the first enzymatic hydrolysis process further includes 0-0.20% calcium chloride; the percentage refers to the mass percentage relative to the plant emulsion.

[0113] In some embodiments of the present invention, the enzyme activity concentration of the high-temperature α-amylase is 9000–13500 U / mL.

[0114] In some embodiments of the present invention, the content of the high-temperature α-amylase is 0.03 to 0.09% relative to the plant emulsion, or for example, 0.06 to 0.09%; the percentage refers to the mass percentage relative to the plant emulsion.

[0115] In this invention, the high-temperature α-amylase refers to an α-amylase derived from thermophilic microorganisms (such as thermophilic bacteria or archaea) or their genetically engineered strains. Its greatest characteristic is that it has extremely high thermal stability and catalytic activity at high temperatures (usually above 90°C), and can efficiently and randomly hydrolyze the α-1,4-glycosidic bonds inside starch molecules, degrading starch into dextrins, oligosaccharides, and small amounts of glucose, maltose, and other small molecule products.

[0116] In some embodiments of the present invention, the high-temperature α-amylase is derived from Bacillus licheniformis.

[0117] In some embodiments of the present invention, the high-temperature α-amylase is sourced from St. Johns Foods Ingredients (Suzhou) Co., Ltd., and the product is Optizym HitaA 17105A, which is derived from Bacillus licheniformis and has the batch number 2326 / 24.10.2024.

[0118] In this invention, those skilled in the art can determine the enzymatic hydrolysis temperature using known methods. The temperature range can fluctuate within a certain range, for example, within ±2°C.

[0119] In some embodiments of the present invention, the first enzymatic hydrolysis is carried out under the action of 0-0.20% calcium chloride and α-amylase with an enzyme activity concentration of 9000-13500 U / mL at high temperature; the percentage refers to the mass percentage relative to the plant emulsion.

[0120] In some embodiments of the present invention, the first enzymatic hydrolysis is carried out under the action of 0-0.20% calcium chloride and 0.06-0.09% high-temperature α-amylase; the percentage refers to the mass percentage relative to the plant emulsion.

[0121] In some embodiments of the present invention, the temperature of the first enzymatic hydrolysis is 85±2℃~90°±2℃, for example 85±2℃.

[0122] In some embodiments of the present invention, the first enzymatic hydrolysis takes 1 to 2 hours.

[0123] In some embodiments of the present invention, the pH of the second enzymatic hydrolysis is 5.0±0.2 to 6.0±0.2, for example 5.3±0.2 to 6.0±0.2, or for example 5.5±0.2 to 6.0±0.2, or even for example 5.0±0.2, 5.3±0.2, 5.5±0.2 or 6.0±0.2.

[0124] In some embodiments of the present invention, the enzyme activity concentration of the α-glucosidase is 180,000 to 720,000 U / mL, for example 180,000 to 540,000 U / mL, or even 180,000 to 360,000 U / mL, or even 180,000 U / mL, 360,000 U / mL, 540,000 U / mL or 720,000 U / mL.

[0125] In some embodiments of the present invention, the content of the α-glucosidase relative to the saccharification solution is 0.12 to 0.48%, for example 0.12 to 0.36%, or for example 0.12 to 0.24%, or even for example 0.12%, 0.24%, or 0.36%.

[0126] In this invention, the α-glucosidase is an exoglycosidic hydrolase that specifically catalyzes the hydrolysis of α-glucosidic bonds, cleaving individual glucose molecules one by one from the non-reducing ends of oligosaccharides (such as maltose and sucrose) or polysaccharides. Its core function is to convert disaccharides and oligosaccharides into directly absorbable glucose.

[0127] In some embodiments of the present invention, the α-glucosidase is derived from Aspergillus niger.

[0128] In some embodiments of the present invention, the α-glucosidase is derived from Amano Enzyme Manufacturing (China), Ltd., and the product is Transglucosidase L”Amano”C, which is derived from Aspergillus niger and has the batch number CTGV1152401L.

[0129] In some embodiments of the present invention, the content of the cellulase is ≥0.05%, where the percentage refers to the mass percentage relative to the saccharification solution.

[0130] In some embodiments of the present invention, the content of the cellulase is ≥0.1%, where the percentage refers to the mass percentage relative to the saccharification solution.

[0131] In some embodiments of the present invention, the second enzymatic hydrolysis is carried out under the action of α-glucosidase with an enzyme activity concentration of 180,000 to 720,000 U / mL and at least 0.05% cellulase, where the percentage refers to the mass percentage relative to the saccharification solution.

[0132] In some embodiments of the present invention, the second enzymatic hydrolysis is carried out with 0.12 to 0.48% α-glucosidase and at least 0.05% cellulase, where percentage refers to the mass percentage relative to the saccharified solution.

[0133] In this invention, the cellulase is a complex enzyme system composed of multiple hydrolases that can synergistically degrade cellulose (β-1,4-glucan) into smaller molecules such as glucose and cellobiose. Its function is to break down the main structural components of plant cell walls.

[0134] In some embodiments of the present invention, the cellulase is sourced from Sternzym Food Ingredients (Suzhou) Co., Ltd., and the product is Sternzym C 29018 cellulase with batch number 0241 / 22.02.2024.

[0135] In some embodiments of the present invention, the temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, for example 55±2℃~60±2℃ or 60±2℃~65±2℃, or even 55±2℃, 60±2℃ or 65±2℃.

[0136] In some embodiments of the present invention, the second enzymatic hydrolysis takes 5 to 10 hours, for example, 5 to 7 hours.

[0137] In some embodiments of the present invention, the grinding of the enzymatic hydrolysate is carried out in a wet ultrafine pulverizer.

[0138] In this invention, the wet ultrafine pulverizer can be a conventional wet ultrafine pulverizer in the art, such as the wet ultrafine pulverizer from Wuxi Hepu Light Industry Equipment Technology Co., Ltd., model HOP-L2.

[0139] In some embodiments of the present invention, the processing capacity of the wet ultrafine pulverizer is 15-25 kg / h, for example 20 kg / h.

[0140] In this invention, the motor power of the wet ultrafine pulverizer can be 1.0 to 5.0 kW, for example 2.2 kW.

[0141] In this invention, the rotational speed of the wet ultrafine pulverizer can be 5000–6000 r / min, for example 5500 r / min.

[0142] In some embodiments of the present invention, the grinding process of the enzymatic hydrolysate is wet grinding.

[0143] In some embodiments of the present invention, the enzymatic hydrolysate is ground three times.

[0144] In some embodiments of the present invention, the average particle size of the substances in the enzymatic hydrolysate after homogenization is ≤100 μm, for example ≤60 μm, or for example 56.96 μm.

[0145] In some embodiments of the present invention, the homogenization pressure is 300 to 500 bar, for example 400 bar.

[0146] In some embodiments of the present invention, the homogenization process further includes sieving and enzyme inactivation.

[0147] The sieving process can be a 100-mesh sieve.

[0148] The enzyme inactivation process can be achieved by maintaining the temperature at 110°C for 10 minutes.

[0149] The enzyme inactivation process can also simultaneously sterilize the enzyme hydrolysate.

[0150] In a preferred embodiment of the present invention, the post-processing includes the following steps:

[0151] The enzymatic hydrolysate was ground in a wet ultrafine pulverizer, homogenized at 400 bar pressure using a homogenizer, passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain the composition containing soluble plant dietary fiber.

[0152] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0153] S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion.

[0154] S2: Under the conditions of pH 5.0±0.2~6.0±0.2, the saccharified solution is subjected to a second enzymatic hydrolysis under the action of cellulase and α-glucosidase with an enzyme activity concentration of 180000~720000 U / mL to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution.

[0155] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0156] S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0.03~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The percentage refers to the mass percentage relative to the plant emulsion.

[0157] S2: Under pH conditions of 5.0±0.2 to 6.0±0.2, the saccharified solution is subjected to a second enzymatic hydrolysis in the presence of cellulase and 0.12 to 0.48% α-glucosidase to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃ to 65±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours. The percentage refers to the mass percentage relative to the saccharified solution.

[0158] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0159] S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0~0.20% calcium chloride and α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion, and the percentage refers to the mass percentage relative to the plant emulsion.

[0160] S2: Under conditions of pH 5.0±0.2~6.0±0.2, and with the action of α-glucosidase at an enzyme activity concentration of 180000~720000 U / mL and at least 0.05% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, and the time of the second enzymatic hydrolysis is 5~10 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution, and the percentage refers to the mass percentage of the saccharified solution.

[0161] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0162] S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0~0.20% calcium chloride and 0.03~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The percentage refers to the mass percentage relative to the plant emulsion.

[0163] S2: Under conditions of pH 5.0±0.2 to 6.0±0.2, and with the action of 0.12 to 0.48% α-glucosidase and at least 0.05% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃ to 65±2℃, and the time of the second enzymatic hydrolysis is 5 to 10 hours. The percentage refers to the mass percentage relative to the saccharified solution.

[0164] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0165] S1: Under conditions of pH 6.7±0.2~7.0±0.2, and with the action of 0~0.15% calcium chloride and α-amylase at a high temperature of 9000~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 85±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion, and the percentage refers to the mass percentage relative to the plant emulsion.

[0166] S2: Under conditions of pH 5.5±0.2~6.0±0.2, and with the action of α-glucosidase at an enzyme activity concentration of 180000~360000 U / mL and at least 0.1% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~60±2℃, and the time of the second enzymatic hydrolysis is 5~7 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution, and the percentage refers to the mass percentage of the saccharified solution.

[0167] In a preferred embodiment of the present invention, the two-step enzymatic hydrolysis treatment includes the following steps:

[0168] S1: Under conditions of pH 6.7±0.2~7.0±0.2, and with the action of 0~0.15% calcium chloride and 0.06~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 85±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The percentage refers to the mass percentage relative to the plant emulsion.

[0169] S2: Under conditions of pH 5.5±0.2~6.0±0.2, and with the action of 0.12~0.24% α-glucosidase and at least 0.1% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~60±2℃, and the time of the second enzymatic hydrolysis is 5~7 hours. The percentage refers to the mass percentage relative to the saccharified solution.

[0170] In some embodiments of the present invention, the composition containing soluble plant dietary fiber includes a prebiotic selected from isomaltooligosaccharides.

[0171] In some embodiments of the present invention, the degree of polymerization of the isomaltooligosaccharide is 2 to 9.

[0172] In some embodiments of the present invention, the isomaltooligosaccharide content in the composition containing soluble plant dietary fiber is ≥6.0g / 100g, for example 6.0-15.0g / 100g, and also for example 6.7g / 100g, 6.8g / 100g, 7.2g / 100g, 7.3g / 100g, 8.0g / 100g, 8.4g / 100g, 9.6g / 100g, 10.0g / 100g, 10.9g / 100g, 11.0g / 100g, 11.9g / 100g, 13.1g / 100g, 13.4g / 100g, 14.5g / 100g, or 14.8g / 100g.

[0173] In some embodiments of the present invention, the soluble plant dietary fiber is a carbohydrate polymer with a degree of polymerization ≥3 that cannot be digested and absorbed by the human small intestine.

[0174] In some embodiments of the present invention, the soluble plant dietary fiber content in the composition is ≥3.00g / 100g, for example 3.00-6.00g / 100g, or for example 3.03g / 100g, 3.38g / 100g, 3.43g / 100g, 3.57g / 100g, 3.59g / 100g, 3.72g / 100g, 3.88g / 100g, 3.92g / 100g, 3.98g / 100g, 4.25g / 100g, 4.31g / 100g, 4.46g / 100g, 4.52g / 100g, 4.67g / 100g, or 5.25g / 100g.

[0175] In some embodiments of the present invention, the maltose content in the composition containing soluble plant dietary fiber is less than 0.5g / 100g.

[0176] In some embodiments of the present invention, the glucose content in the composition containing soluble plant dietary fiber is ≤13.5g / 100g, for example 4.0-13.5g / 100g, and also for example 4.8g / 100g, 5.0g / 100g, 6.6g / 100g, 6.8g / 100g, 6.9g / 100g, 7.6g / 100g, 8.3g / 100g, 9.5g / 100g, 9.9g / 100g, 10.8g / 100g, 11.2g / 100g, 11.5g / 100g, 12.1g / 100g, 12.3g / 100g, or 13.5g / 100g.

[0177] In some embodiments of the present invention, the water content of the composition containing soluble plant dietary fiber is 70-80%, for example 71.6%, where the percentage refers to the weight percentage of the composition containing soluble plant dietary fiber.

[0178] The sources of raw materials in the following examples and comparative examples are as follows:

[0179] Kernel corn refers to fully mature corn kernels, and its nutritional composition is shown in the table below.

[0180] name corn Edible portion, % 100 Moisture content, g / 100g 11.8 Energy, kcal / 100g 327 Protein, g / 100g 8.0 Fat, g / 100g 0.8 Carbohydrates, g / 100g 79.2 Insoluble dietary fiber, g / 100g - Cholesterol, mg / 100g 0 Ash content, g / 100g 0.2 Total Vitamin A, μg / 100g 8 Carotene, μg / 100g 100 Retinol, μg / 100g 0 Thiamine, mg / 100g 0.03 Riboflavin, mg / 100g 0.02 ;

[0181] Brown rice refers to the product of rice after processing and dehulling, such as brown rice that meets GB / T 18810-2002;

[0182] The nutritional components of brown rice are shown in the table below;

[0183] name brown rice Edible portion, % 100 Moisture content, g / 100g 13.4 Energy, kcal / 100g 348 Protein, g / 100g 7.7 Fat, g / 100g 2.7 Carbohydrates, g / 100g 75 Insoluble dietary fiber, g / 100g 3.4 Cholesterol, mg / 100g 0 Ash content, g / 100g 1.2 Total Vitamin A, μg / 100g trace amounts Carotene, μg / 100g trace amounts Retinol, μg / 100g 0 Thiamine, mg / 100g 0.38 Riboflavin, mg / 100g 0.04 ;

[0184] The high-temperature α-amylase was sourced from St. Johns Food Ingredients (Suzhou) Co., Ltd., and the product was OptizymHitaA17105A. The product source was Bacillus licheniformis, and the batch number was 2326 / 24.10.2024.

[0185] The α-glucosidase is derived from Amano Enzyme Manufacturing (China), Ltd., and the product is Transglucosidase L”Amano”C. The product source is Aspergillus niger, and the batch number is CTGV1152401L.

[0186] The cellulase was sourced from Sternzym Food Ingredients (Suzhou) Co., Ltd., and the product was Sternzym C29018, batch number 0241 / 22.02.2024.

[0187] β-amylase was purchased from St. Johns Foods Ingredients (Suzhou) Co., Ltd., product Betamalt 25FBD, batch number 2637 / 28.11.2024;

[0188] Pullulanase was purchased from Sternzym Food Ingredients (Suzhou) Co., Ltd., product name: Sternzym PUG26780L, batch number: 2638 / 28.11.2024.

[0189] In the following embodiments and comparative examples:

[0190] The universal pulverizer is a Baijie multi-functional pulverizer, model: BJ-800A, with a pulverization degree of 50-300 mesh, a frequency of 50Hz, a power of 1400W, and a pulverization process of dry pulverization.

[0191] The ratio of corn flour, brown rice flour, and purified water refers to the mass ratio.

[0192] The colloid mill equipment is a colloid mill from Langfang Langtong Machinery Co., Ltd., model: JMS-500, rotor speed: 2840r / min, motor power: 1.5kw, and the grinding process is wet grinding;

[0193] The content of high-temperature α-amylase refers to the percentage of the plant emulsion by mass after grinding. For example, 0.06% high-temperature α-amylase means that 0.06g of high-temperature α-amylase is added to every 100g of ground plant emulsion.

[0194] The enzyme activity concentration of high-temperature α-amylase refers to the enzyme activity concentration in the plant emulsion after grinding;

[0195] The calcium chloride content refers to the percentage content relative to the mass of the ground plant emulsion. For example, 0.20% calcium chloride means that 0.20g of calcium chloride is added to every 100g of ground plant emulsion.

[0196] The content of α-glucosidase refers to the percentage of α-glucosidase relative to the mass of the saccharification solution. For example, 0.24% α-glucosidase means that 0.24g of α-glucosidase is added per 100g of saccharification solution.

[0197] The enzyme activity concentration of α-glucosidase refers to the enzyme activity concentration in the saccharification solution;

[0198] The cellulase content refers to the percentage of cellulase relative to the mass of the saccharification solution. For example, 0.1% cellulase means that 0.1g of cellulase is added per 100g of saccharification solution.

[0199] The content of β-amylase refers to the percentage of β-amylase relative to the mass of the slurry. For example, 0.5% β-amylase means that 0.5g of β-amylase is added per 100g of slurry.

[0200] Pullulanase content refers to the percentage of pullulanase relative to the mass of the slurry. For example, 0.01% pullulanase means that 0.01g of pullulanase is added per 100g of slurry.

[0201] The wet ultrafine pulverizer is a wet ultrafine pulverizer from Wuxi Hepu Light Industry Equipment Technology Co., Ltd., with a processing capacity of 20kg / h, model number HOP-L2, motor power of 2.2kw, speed of 5500r / min, and a wet grinding process with 3 cycles.

[0202] The homogenization conditions are as follows: the homogenizer is used to homogenize at a pressure of 400 bar;

[0203] The particle size analyzer is: Microtrac S3500, Model S3550, Serial: MW15031310-D6042;

[0204] The method for detecting the content of isomaltooligosaccharide is the same as that shown in GB / T 20881-2017 Isomaltooligosaccharide.

[0205] The method for detecting soluble dietary fiber content is GB 5009.88-2023. The soluble dietary fiber measured in GB 5009.88-2023 is a carbohydrate polymer that cannot be digested and absorbed by the human small intestine and has a degree of polymerization ≥3.

[0206] The method for detecting maltose content is GB 5009.8-2023 Method I, which has a detection limit of 0.5 g / 100 g for maltose. If maltose is not detected, the maltose content is lower than the detection limit, that is, lower than 0.5 g / 100 g.

[0207] The method for detecting glucose content is GB 5009.8-2023 Method I.

[0208] The yield is calculated as follows: In the post-processing step, the mass of the soluble plant dietary fiber composition containing prebiotics before passing through a 100-mesh sieve is weighed as M1, and the mass of the soluble plant dietary fiber composition containing prebiotics after passing through a 100-mesh sieve is weighed as M2. The yield is the ratio of M2 to M1. α-Glucosidase activity assay method and principle:

[0209] Enzyme activity was determined using methyl-α-D-glucoside as a substrate; transglucosidase catalyzed the decomposition of methyl-α-D-glucoside into D-glucose and methanol; then, the glucose formed was measured using a 4-aminoantipyrin-phenol reagent containing glucose oxidase and peroxidase. Enzyme activity units were defined as the amount of enzyme required to produce 1 μg of glucose in 60 minutes under the stated conditions.

[0210] Methods and principles for determining α-amylase activity at high temperatures:

[0211] High-temperature α-amylase solution was added to a soluble starch solution, shaken well, and reacted in a water bath. The reaction solution was then removed and placed in hydrochloric acid solution to terminate the enzymatic hydrolysis. Dilute iodine solution was added, and the solution was shaken well. The absorbance was measured at 660 nm. The concentration of high-temperature α-amylase was calculated based on the enzyme concentration versus absorbance standard curve. Enzyme activity is defined as the amount of enzyme required to liquefy 1 mg of soluble starch in 1 minute.

[0212] Method for testing moisture content: direct drying method.

[0213] In the following embodiments and comparative examples:

[0214] The plant emulsion is obtained by thorough grinding using a colloid mill. The specific process is as follows: after grinding three times with a colloid mill, the slurry is ground until it is uniform and does not separate after being left for a period of time.

[0215] Example 1 (different α-glucosidase hydrolysis times)

[0216] Raw material pretreatment:

[0217] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0218] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0219] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0220] Two-step enzymatic hydrolysis:

[0221] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0222] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0223] Post-processing:

[0224] (6) After fine grinding in a wet ultrafine grinder, the enzymatic hydrolysate is homogenized at a pressure of 400 bar. After fine grinding and homogenization, the average particle size (MV) of the substances in the enzymatic hydrolysate is 56.96 μm. Then, it is passed through a 100-mesh sieve and kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0225] Test results:

[0226] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 1 contains 10g / 100g of isomaltooligosaccharide, 4.67g / 100g of soluble dietary fiber, no detectable maltose, and 6.9g / 100g of glucose; the water content is 71.6%, and the percentage refers to the weight percentage of the prebiotic-rich water-soluble plant dietary fiber. In the post-processing step, after passing through a 100-mesh sieve, the yield is ≥95%.

[0227] Example 2 (different α-glucosidase hydrolysis times)

[0228] Raw material pretreatment:

[0229] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0230] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0231] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0232] Two-step enzymatic hydrolysis:

[0233] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0234] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0235] Post-processing:

[0236] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0237] Test results:

[0238] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 2 contained 10.9 g / 100 g of isomaltooligosaccharide, 3.88 g / 100 g of soluble dietary fiber, no detectable maltose, and 8.3 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0239] Example 3 (different α-glucosidase hydrolysis times)

[0240] Raw material pretreatment:

[0241] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0242] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0243] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0244] Two-step enzymatic hydrolysis:

[0245] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0246] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 5 hours to obtain the enzymatic hydrolysate;

[0247] Post-processing:

[0248] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0249] Test results:

[0250] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 3 contained 11.0 g / 100 g of isomaltooligosaccharide, 3.03 g / 100 g of soluble dietary fiber, no detectable maltose, and 7.6 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0251] Based on the results of Examples 1, 2, and 3 above, it can be concluded that when the α-glucosidase hydrolysis time is 5 to 10 hours, the content of isomaltooligosaccharides generated after 5 hours and 7 hours of hydrolysis is similar, but higher than that generated after 10 hours of hydrolysis.

[0252] Example 4 (different amounts of α-glucosidase added)

[0253] Raw material pretreatment:

[0254] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0255] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0256] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0257] Two-step enzymatic hydrolysis:

[0258] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0259] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0260] Post-processing:

[0261] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0262] Test results:

[0263] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 4 contained 14.5 g / 100 g of isomaltooligosaccharide, 3.57 g / 100 g of soluble dietary fiber, no detectable maltose, and 9.5 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0264] Based on the results of Examples 2 and 4 above, it can be concluded that when the amount of α-glucosidase added is between 0.12% and 0.24%, the content of isomaltooligosaccharide generated by adding 0.12% α-glucosidase is higher than that generated by adding 0.24% α-glucosidase.

[0265] Example 5 (The effect of different pH values ​​on α-glucosidase hydrolysis)

[0266] Raw material pretreatment:

[0267] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0268] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0269] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0270] Two-step enzymatic hydrolysis:

[0271] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0272] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.0±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 60±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0273] Post-processing:

[0274] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0275] Test results:

[0276] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 5 contained 6.7 g / 100 g of isomaltooligosaccharide, 4.25 g / 100 g of soluble dietary fiber, no detectable maltose, and 9.9 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0277] Example 6 (The effect of different pH values ​​on α-glucosidase hydrolysis)

[0278] Raw material pretreatment:

[0279] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0280] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0281] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0282] Two-step enzymatic hydrolysis:

[0283] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0284] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.5±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 60±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0285] Post-processing:

[0286] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0287] Test results:

[0288] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 6 contains 8 g / 100g of isomaltooligosaccharide, 3.92 g / 100g of soluble dietary fiber, no detectable maltose, and 10.8 g / 100g of glucose. In the post-processing step, after passing through a 100-mesh sieve, the yield is ≥95%.

[0289] Example 7 (The effect of different pH values ​​on α-glucosidase hydrolysis)

[0290] Raw material pretreatment:

[0291] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0292] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0293] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0294] Two-step enzymatic hydrolysis:

[0295] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0296] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 6.0±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 60±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0297] Post-processing:

[0298] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0299] Test results:

[0300] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 7 contained 9.6 g / 100 g of isomaltooligosaccharide, 4.31 g / 100 g of soluble dietary fiber, no detectable maltose, and 12.3 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0301] Based on the results of Examples 5, 6, and 7 above, it can be concluded that within the pH range of α-glucosidase hydrolysis (5.0–6.0), the higher the hydrolysis pH, the higher the content of isomaltooligosaccharides produced.

[0302] Example 8 (The effect of different temperatures on α-glucosidase hydrolysis)

[0303] Raw material pretreatment:

[0304] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0305] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0306] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0307] Two-step enzymatic hydrolysis:

[0308] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0309] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.0±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0310] Post-processing:

[0311] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0312] Test results:

[0313] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 8 contained 7.2 g / 100 g of isomaltooligosaccharide, 4.52 g / 100 g of soluble dietary fiber, no detectable maltose, and 11.5 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0314] Example 9 (The effect of different temperatures on α-glucosidase hydrolysis)

[0315] Raw material pretreatment:

[0316] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0317] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0318] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0319] Two-step enzymatic hydrolysis:

[0320] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0321] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.0±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 65±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0322] Post-processing:

[0323] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0324] Test results:

[0325] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 9 contained 6.8 g / 100 g of isomaltooligosaccharide, 5.25 g / 100 g of soluble dietary fiber, no detectable maltose, and 13.5 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0326] Based on the results of Examples 5, 8, and 9 above, it can be concluded that when the α-glucosidase hydrolysis temperature is between 55 and 65°C, the content of isomaltooligosaccharides generated at hydrolysis temperatures of 65°C and 60°C is similar, but lower than that generated at hydrolysis temperature of 55°C.

[0327] Example 10 (Effect of different amounts of α-glucosidase added)

[0328] Raw material pretreatment:

[0329] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0330] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0331] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0332] Two-step enzymatic hydrolysis:

[0333] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0334] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.36% of α-glucosidase (0.36% corresponds to an enzyme activity concentration of 540000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0335] Post-processing:

[0336] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0337] Test results:

[0338] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 10 contained 8.4 g / 100 g of isomaltooligosaccharide, 4.46 g / 100 g of soluble dietary fiber, no detectable maltose, and 12.1 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0339] Example 11 (Effect of different amounts of α-glucosidase added)

[0340] Raw material pretreatment:

[0341] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0342] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0343] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0344] Two-step enzymatic hydrolysis:

[0345] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0346] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.48% of α-glucosidase (the amount added is 0.48% corresponding to the enzyme activity concentration: 720000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0347] Post-processing:

[0348] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0349] Test results:

[0350] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 11 contained 7.3 g / 100 g of isomaltooligosaccharide, 3.98 g / 100 g of soluble dietary fiber, no detectable maltose, and 11.2 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0351] Based on the results of Examples 1, 10, and 11 above, it can be concluded that when the amount of α-glucosidase added is between 0.24% and 0.48%, the lower the amount of α-glucosidase added, the higher the content of isomaltooligosaccharide generated.

[0352] Example 12 (The effect of different amounts of α-amylase added at high temperatures)

[0353] Raw material pretreatment:

[0354] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0355] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0356] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0357] Two-step enzymatic hydrolysis:

[0358] (4) Adjust the pH to 6.7±0.2, add 0.03% high-temperature α-amylase (0.03% corresponds to an enzyme activity concentration of 4500U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0359] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0360] Post-processing:

[0361] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0362] Test results:

[0363] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 12 contained 11.9 g / 100 g of isomaltooligosaccharide, 3.59 g / 100 g of soluble dietary fiber, no detectable maltose, and 6.6 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0364] Example 13 (The effect of different amounts of α-amylase added at high temperatures)

[0365] Raw material pretreatment:

[0366] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0367] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0368] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0369] Two-step enzymatic hydrolysis:

[0370] (4) Adjust the pH to 6.7±0.2, add 0.09% of high-temperature α-amylase (0.09% corresponds to an enzyme activity concentration of 13500U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0371] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0372] Post-processing:

[0373] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0374] Test results:

[0375] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 13 contained 14.8 g / 100 g of isomaltooligosaccharide, 3.38 g / 100 g of soluble dietary fiber, no detectable maltose, and 6.8 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0376] Based on the results of Examples 4, 12, and 13 above, it can be concluded that when the amount of high-temperature α-amylase added is between 0.03% and 0.09%, the content of isomaltooligosaccharide generated by adding 0.06% and 0.09% of high-temperature α-amylase is higher than that generated by adding 0.03% of high-temperature α-amylase.

[0377] Example 14 (Effect of different calcium chloride concentrations added during high-temperature α-amylase hydrolysis)

[0378] Raw material pretreatment:

[0379] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0380] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0381] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0382] Two-step enzymatic hydrolysis:

[0383] (4) Adjust the pH to 6.7±0.2, add 0.06% high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL) and 0.15% calcium chloride powder, adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain saccharified solution;

[0384] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0385] Post-processing:

[0386] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0387] Test results:

[0388] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 14 contained 13.1 g / 100 g of isomaltooligosaccharide, 3.43 g / 100 g of soluble dietary fiber, no detectable maltose, and 5.0 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0389] Example 15 (Effect of different calcium chloride concentrations added during high-temperature α-amylase hydrolysis)

[0390] Raw material pretreatment:

[0391] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0392] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0393] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0394] Two-step enzymatic hydrolysis:

[0395] (4) Adjust the pH to 6.7±0.2, add 0.06% high-temperature α-amylase (addition amount of 0.06% corresponds to enzyme activity concentration: 9000U / mL) and 0.20% calcium chloride powder, adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain saccharified solution;

[0396] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0397] Post-processing:

[0398] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0399] Test results:

[0400] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Example 15 contained 13.4 g / 100 g of isomaltooligosaccharide, 3.72 g / 100 g of soluble dietary fiber, no detectable maltose, and 4.8 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0401] Based on the results of Examples 4, 14, and 15 above, it can be concluded that during the high-temperature α-amylase hydrolysis process, the content of isomaltooligosaccharides generated without the addition of calcium chloride powder is higher than the content of isomaltooligosaccharides generated with the addition of 0.15% and 0.2% calcium chloride powder.

[0402] Comparative Example 1 (Effect of different α-glucosidase hydrolysis times)

[0403] Raw material pretreatment:

[0404] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0405] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0406] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0407] Two-step enzymatic hydrolysis:

[0408] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0409] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 3 hours to obtain the enzymatic hydrolysate;

[0410] Post-processing:

[0411] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0412] Test results:

[0413] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 1 contained 4.6 g / 100 g of isomaltooligosaccharide, 2.39 g / 100 g of soluble dietary fiber, no detectable maltose, and 9.8 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0414] Based on the results of Examples 1, 2, and 3 and Comparative Example 1, it can be concluded that when the enzymatic hydrolysis time of the saccharification solution is less than 5 hours, the content of the generated isomaltooligosaccharide is significantly reduced. The content of isomaltooligosaccharide generated after 5 hours of enzymatic hydrolysis is 2.4 times that of isomaltooligosaccharide generated after 3 hours of enzymatic hydrolysis.

[0415] Comparative Example 2 (Effect of Different Amounts of α-glucosidase Added)

[0416] Raw material pretreatment:

[0417] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0418] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0419] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0420] Two-step enzymatic hydrolysis:

[0421] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0422] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.06% of α-glucosidase (0.06% corresponds to an enzyme activity concentration of 90000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0423] Post-processing:

[0424] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0425] Test results:

[0426] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 2 contained 3.7 g / 100 g of isomaltooligosaccharide, 3.82 g / 100 g of soluble dietary fiber, no detectable maltose, and 11.3 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0427] Based on the results of Examples 2 and 4 and Comparative Example 2, it can be concluded that when the amount of α-glucosidase added is ≤0.06%, the content of isomaltooligosaccharide generated is significantly reduced. The content of isomaltooligosaccharide generated by adding 0.12% α-glucosidase is 3.9 times that of isomaltooligosaccharide generated by adding 0.06% α-glucosidase.

[0428] Comparative Example 3 (The effect of different pH values ​​on α-glucosidase hydrolysis)

[0429] Raw material pretreatment:

[0430] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0431] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0432] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0433] Two-step enzymatic hydrolysis:

[0434] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0435] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 6.5±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0436] Post-processing:

[0437] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0438] Test results:

[0439] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 3 contained 5.6 g / 100 g of isomaltooligosaccharide, 4.08 g / 100 g of soluble dietary fiber, no detectable maltose, and 9.6 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0440] Based on the results of Examples 1 and 8 and Comparative Example 3, it can be concluded that when α-glucosidase hydrolysis is performed at pH 6.5 ± 0.2, the content of isomaltooligosaccharides generated is significantly reduced. The content of isomaltooligosaccharides generated when α-glucosidase hydrolysis is performed at pH 5.3 ± 0.2 is 1.8 times that of isomaltooligosaccharides generated when hydrolysis is performed at pH 6.5 ± 0.2.

[0441] Comparative Example 4 (The effect of different temperatures on α-glucosidase hydrolysis)

[0442] Raw material pretreatment:

[0443] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0444] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0445] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0446] Two-step enzymatic hydrolysis:

[0447] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0448] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.0±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 70±2℃, and enzymatically hydrolyze for 10 hours to obtain the enzymatic hydrolysate;

[0449] Post-processing:

[0450] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0451] Test results:

[0452] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 4 contained 4.7 g / 100 g of isomaltooligosaccharide, 2.67 g / 100 g of soluble dietary fiber, no detectable maltose, and 12.8 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0453] Based on the results of Examples 5, 8, and 9 and Comparative Example 4, it can be concluded that when the α-glucosidase hydrolysis temperature is 70±2℃, the content of the generated isomaltooligosaccharide is significantly reduced, and the content of the generated isomaltooligosaccharide when the α-glucosidase hydrolysis temperature is 65±2℃ is 1.4 times that of the content of the generated isomaltooligosaccharide when the hydrolysis temperature is 70±2℃.

[0454] Comparative Example 5 (Effect of different amounts of α-amylase added at high temperatures)

[0455] Raw material pretreatment:

[0456] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0457] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0458] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0459] Two-step enzymatic hydrolysis:

[0460] (4) Adjust the pH to 6.7±0.2, add 0.01% of high-temperature α-amylase (0.01% corresponds to an enzyme activity concentration of 1500U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0461] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.12% of α-glucosidase (0.12% corresponds to an enzyme activity concentration of 180000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0462] Post-processing:

[0463] (6) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0464] Test results:

[0465] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 5 contained 3.1 g / 100 g of isomaltooligosaccharide, 3.90 g / 100 g of soluble dietary fiber, no detectable maltose, and 13.2 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0466] Based on the results of Examples 4, 12, 13 and Comparative Example 5, it can be concluded that when the amount of high-temperature α-amylase added is 0.01%, the content of isomaltooligosaccharides generated is significantly reduced. The content of isomaltooligosaccharides generated when 0.03% of high-temperature α-amylase is added is 3.8 times that when 0.01% of high-temperature α-amylase is added, the content of isomaltooligosaccharides generated ...01% that when 0.01% of high-temperature α-amylase is added, the content of isomaltooligo

[0467] Comparative Example 6 (without the effect of α-glucosidase hydrolysis process)

[0468] Raw material pretreatment:

[0469] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0470] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0471] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0472] One-step enzymatic hydrolysis:

[0473] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0474] Post-processing:

[0475] (5) After the saccharified liquid in (4) is put into a wet ultrafine pulverizer for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain the product.

[0476] Test results:

[0477] According to the production process of Comparative Example 6, the water-soluble plant dietary fiber rich in prebiotics was not found to contain isomaltooligosaccharide or soluble dietary fiber. The maltose content was 7.8g / 100g and the glucose content was 4.6g / 100g.

[0478] Based on the results of Examples 1-11 and Comparative Example 6, it can be concluded that there is no α-glucosidase enzymatic hydrolysis process and no oligoisomaltose is generated.

[0479] Comparative Example 7 (Effects of pullulanase & β-amylase)

[0480] Raw material pretreatment:

[0481] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0482] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0483] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0484] Two-step enzymatic hydrolysis:

[0485] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and maintain for 10 minutes;

[0486] (5) Use anhydrous citric acid to adjust the pH of the slurry in (4) to 4.5±0.2, and adjust the enzymatic hydrolysis temperature to 55±2℃, then add 0.01% pullulanase and 0.5% β-amylase, and enzymatically hydrolyze for 1 hour;

[0487] (6) Use food-grade baking soda to adjust the pH of the saccharification solution in (5) to 5.5±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatic hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0488] Post-processing:

[0489] (7) After the enzymatic hydrolysate is put into a wet ultrafine grinder for fine grinding, it is homogenized by a homogenizer at a pressure of 400 bar, then passed through a 100-mesh sieve, and then kept at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0490] Test results:

[0491] The prebiotic-rich water-soluble plant dietary fiber obtained according to the production process of Comparative Example 7 contained 7.0 g / 100 g of isomaltooligosaccharide, 1.80 g / 100 g of soluble dietary fiber, no detectable maltose, and 16.0 g / 100 g of glucose. In the post-processing steps, after passing through a 100-mesh sieve, the yield was ≥95%.

[0492] Because pullulanase and β-amylase were added to the original process, and an additional enzymatic hydrolysis step was added, production efficiency decreased and raw material costs increased. However, the content of isomaltooligosaccharide and soluble dietary fiber in the produced product did not increase significantly, and the content of soluble dietary fiber even decreased.

[0493] Based on the results of Example 2 and Comparative Example 7, it can be concluded that the content of isomaltooligosaccharide generated in Comparative Example 7 did not increase, and even decreased. The content of isomaltooligosaccharide generated in Example 2 was 1.6 times that of isomaltooligosaccharide generated in Comparative Example 7.

[0494] Comparative Example 8 (no fine grinding or homogenization in post-processing)

[0495] Raw material pretreatment:

[0496] (1) Wash the corn kernels and brown rice kernels clean, and grind them separately using a universal grinder to obtain corn flour and brown rice flour;

[0497] (2) Prepare the plant emulsion by mixing corn flour, brown rice flour and purified water in a ratio of 2:2:6.

[0498] (3) The plant emulsion in (2) is thoroughly ground using a colloid mill to obtain the ground plant emulsion.

[0499] Two-step enzymatic hydrolysis:

[0500] (4) Adjust the pH to 6.7±0.2, add 0.06% of high-temperature α-amylase (0.06% corresponds to an enzyme activity concentration of 9000U / mL), adjust the enzymatic hydrolysis temperature to 85±2℃, and enzymatically hydrolyze for 1 hour to obtain the saccharified solution;

[0501] (5) Use anhydrous citric acid to adjust the pH of the saccharification solution in (4) to 5.3±0.2, add 0.24% of α-glucosidase (the amount added is 0.24% corresponding to the enzyme activity concentration: 360000U / mL) and 0.1% of cellulase, adjust the enzymatic hydrolysis temperature to 55±2℃, and enzymatically hydrolyze for 7 hours to obtain the enzymatic hydrolysate;

[0502] Post-processing:

[0503] (6) Pass the enzymatic hydrolysate through a 100-mesh sieve and keep it at 110°C for 10 minutes to obtain a water-soluble plant dietary fiber composition rich in prebiotics.

[0504] Test results:

[0505] In the post-processing steps of this experiment, when passing through a 100-mesh sieve, the yield was only about 60%, resulting in significant losses and making it unsuitable for industrial production.

[0506] Based on the results of Example 2 and Comparative Example 8, it can be concluded that without fine grinding and homogenization in the post-processing, the yield is only about 60%, resulting in significant losses and making it unsuitable for industrial production.

[0507] As shown above, implementing this technical solution can produce a composite product containing isomaltooligosaccharide and soluble dietary fiber, wherein the isomaltooligosaccharide content is greater than or equal to 6g / 100g and the soluble dietary fiber content is greater than or equal to 3g / 100g. Since all corn kernels and brown rice kernels are utilized in the production process, the utilization rate of raw materials is significantly improved; the overall production time is controlled within 12 hours, resulting in relatively high production efficiency.

Claims

1. A preparation process for a composition containing soluble plant dietary fiber, characterized in that, It includes the following steps: (1) Obtaining raw materials: plant emulsion, wherein the plant emulsion contains corn flour, brown rice flour and water; (2) Two-step enzymatic hydrolysis: S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion. S2: Under conditions of pH 4.5±0.2~6.2±0.2, and with the action of cellulase and α-glucosidase with an enzyme activity concentration ≥180000U / mL, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 50±2℃~67±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours; the enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution. (3) Post-processing: The enzymatic hydrolysate is ground and homogenized to obtain a composition containing soluble plant dietary fiber.

2. The preparation process of the composition containing soluble plant dietary fiber as described in claim 1, characterized in that, The two-step enzymatic hydrolysis process satisfies one or more of the following conditions: a. The pH of the first enzymatic hydrolysis was 6.7±0.2 to 7.0±0.2; b. The first enzymatic hydrolysis process also includes 0-0.20% calcium chloride; the percentage refers to the mass percentage relative to the plant emulsion. c. The enzyme activity concentration of the high-temperature α-amylase is 9000–13500 U / mL; d. The content of the high-temperature α-amylase is 0.03-0.09% relative to the plant emulsion; e. The high-temperature α-amylase is derived from Bacillus licheniformis; f. The temperature of the first enzymatic hydrolysis is 85±2℃~90°±2℃; g. The first enzymatic hydrolysis time is 1-2 hours; h. The pH of the second enzymatic hydrolysis is 5.0±0.2 to 6.0±0.2; i. The enzyme activity concentration of the α-glucosidase is 180,000 to 720,000 U / mL; j. The content of α-glucosidase relative to the saccharification solution is at least 0.12%, preferably 0.12-0.48%; k. The α-glucosidase is derived from Aspergillus niger; 1. The content of the cellulase is at least 0.05%, and the percentage refers to the mass percentage relative to the saccharification solution; m. The temperature for the second enzymatic hydrolysis is 55±2℃~65±2℃; n. The second enzymatic hydrolysis takes 5–10 hours; and o. The two-step enzymatic hydrolysis process does not contain β-amylase and / or pullulanase.

3. The preparation process of the composition containing soluble plant dietary fiber as described in claim 1 or 2, characterized in that, The two-step enzymatic hydrolysis process satisfies one or more of the following conditions: a. The first enzymatic hydrolysis process also includes 0-0.15% calcium chloride; the percentage refers to the mass percentage relative to the plant emulsion. b. The content of the high-temperature α-amylase is 0.06-0.09% relative to the plant emulsion; c. The pH of the second enzymatic hydrolysis is 5.3±0.2 to 6.0±0.2; d. The content of α-glucosidase relative to the saccharification solution is 0.12-0.36%; e. The enzyme activity concentration of the α-glucosidase is 180,000–540,000 U / mL; f. The content of the cellulase is at least 0.10%, where the percentage refers to the mass percentage relative to the saccharification solution; g. The temperature for the second enzymatic hydrolysis is 55±2℃~60±2℃ or 60±2℃~65±2℃; and h. The second enzymatic hydrolysis takes 5 to 7 hours.

4. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 3, characterized in that, The preparation process satisfies one or more of the following conditions: a. The plant emulsion is ground using a colloid mill; b. The mass ratio of the corn flour, the brown rice flour, and the water is (1.25–2.25):(1.25–2.25):(5.5–6.5); c. The enzyme activity concentration of the α-glucosidase is 180,000–360,000 U / mL; d. The content of α-glucosidase relative to the saccharification solution is 0.12-0.24%; e. The average particle size of the substances in the homogenized enzymatic hydrolysate is ≤100μm; f. The pressure of the homogenized mass is 300–500 bar; g. The homogenization process further includes sieving and enzyme inactivation. h. The composition containing soluble plant dietary fiber includes a prebiotic selected from isomaltooligosaccharides; i. Mix the corn flour, the brown rice flour, and the water evenly to obtain the plant emulsion; and j. The insoluble dietary fiber content in the brown rice flour is 3.0–6.0 g / 100 g.

5. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 4, characterized in that, The two-step enzymatic hydrolysis process includes the following steps: S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion. S2: Under conditions of pH 5.0±0.2~6.0±0.2, and with the action of cellulase and α-glucosidase with an enzyme activity concentration of 180000~720000 U / mL, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution. or, S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0.03~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃. The percentage refers to the mass percentage relative to the plant emulsion. S2: Under pH conditions of 5.0±0.2 to 6.0±0.2, the saccharified solution is subjected to a second enzymatic hydrolysis in the presence of cellulase and 0.12 to 0.48% α-glucosidase to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃ to 65±2℃, and the time of the second enzymatic hydrolysis is at least 5 hours. The percentage refers to the mass percentage relative to the saccharified solution.

6. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 4, characterized in that, The two-step enzymatic hydrolysis process includes the following steps: S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0~0.20% calcium chloride and α-amylase at a high temperature of 4500~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion, and the percentage refers to the mass percentage relative to the plant emulsion. S2: Under conditions of pH 5.0±0.2~6.0±0.2, and with the action of α-glucosidase at an enzyme activity concentration of 180000~720000 U / mL and at least 0.05% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~65±2℃, and the time of the second enzymatic hydrolysis is 5~10 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution, and the percentage refers to the mass percentage relative to the saccharified solution. or, S1: Under conditions of pH 6.5±0.2~7.0±0.2, and with the action of 0~0.20% calcium chloride and 0.03~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 80±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The percentage refers to the mass percentage relative to the plant emulsion. S2: Under conditions of pH 5.0±0.2 to 6.0±0.2, and with the action of 0.12 to 0.48% α-glucosidase and at least 0.05% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃ to 65±2℃, and the time of the second enzymatic hydrolysis is 5 to 10 hours. The percentage refers to the mass percentage relative to the saccharified solution.

7. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 6, characterized in that, The two-step enzymatic hydrolysis process includes the following steps: S1: Under conditions of pH 6.7±0.2~7.0±0.2, and with the action of 0~0.15% calcium chloride and α-amylase at a high temperature of 9000~13500U / mL, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 85±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The enzyme activity concentration refers to the enzyme activity concentration in the plant emulsion, and the percentage refers to the mass percentage relative to the plant emulsion. S2: Under conditions of pH 5.5±0.2~6.0±0.2, and with the action of α-glucosidase at an enzyme activity concentration of 180000~360000 U / mL and at least 0.1% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~60±2℃, and the time of the second enzymatic hydrolysis is 5~7 hours. The enzyme activity concentration refers to the enzyme activity concentration in the saccharified solution, and the percentage refers to the mass percentage relative to the saccharified solution. or, S1: Under conditions of pH 6.7±0.2~7.0±0.2, and with the action of 0~0.15% calcium chloride and 0.06~0.09% high-temperature α-amylase, the plant emulsion is subjected to a first enzymatic hydrolysis to obtain a saccharified solution. The temperature of the first enzymatic hydrolysis is 85±2℃~90±2℃, and the time of the first enzymatic hydrolysis is 1~2 hours. The percentage refers to the mass percentage relative to the plant emulsion. S2: Under conditions of pH 5.5±0.2~6.0±0.2, and with the action of 0.12~0.24% α-glucosidase and at least 0.1% cellulase, the saccharified solution is subjected to a second enzymatic hydrolysis to obtain an enzymatic hydrolysate. The temperature of the second enzymatic hydrolysis is 55±2℃~60±2℃, and the time of the second enzymatic hydrolysis is 5~7 hours. The percentage refers to the mass percentage relative to the saccharified solution.

8. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 7, characterized in that, In the composition containing soluble plant dietary fiber: Isomaltooligosaccharide content ≥6.0g / 100g; Soluble plant dietary fiber content ≥3.00g / 100g; Maltose content is less than 0.5g / 100g.

9. The preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 8, characterized in that, The composition containing soluble plant dietary fiber satisfies one or more of the following conditions: a. In the composition containing soluble plant dietary fiber: the glucose content is ≤13.5g / 100g; b. The content of the isomaltooligosaccharide is 6.0–15.0 g / 100 g; c. The degree of polymerization of the isomaltooligosaccharide is 2 to 9; d. The method for detecting the content of isomaltooligosaccharide is GB / T 20881-2017; e. The soluble plant dietary fiber content is 3.00–6.00 g / 100 g; f. The soluble plant dietary fiber is a carbohydrate polymer that cannot be digested and absorbed by the human small intestine and has a degree of polymerization ≥3; g. The method for detecting the soluble plant dietary fiber content is GB 5009.88-2023; and h. The water content of the composition containing soluble plant dietary fiber is 70-80%, and the percentage refers to the weight percentage of the composition containing soluble plant dietary fiber.

10. A composition containing soluble plant dietary fiber, characterized in that, It is prepared using the preparation process of the composition containing soluble plant dietary fiber as described in any one of claims 1 to 9.

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