A traditional Chinese medicine composition for preventing and treating liver injury and a preparation method thereof

Abstract

The application belongs to the technical field of medicine, and particularly discloses a traditional Chinese medicine composition for preventing and treating liver injury and a preparation method thereof. The traditional Chinese medicine composition comprises 10-14 parts of radix bupleuri, 12-18 parts of honeysuckle, 15-30 parts of radix astragali, 8-12 parts of rhizoma polygonati, 8-12 parts of longan arillus, 8-12 parts of licorice, 8-12 parts of fried fructus gardeniae, and 8-12 parts of semen plantaginis. The composition has the effect of protecting liver injury, and the preparation method is simple and convenient to use.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a traditional Chinese medicine composition for preventing and treating liver damage and its preparation method. Background Technology

[0002] The liver's main functions are storing glycogen, secreting bile, regulating the metabolism of proteins, fats, and carbohydrates, and playing a role in detoxification, hematopoiesis, and blood clotting. It is the largest detoxification organ in the body, breaking down toxic substances absorbed from the intestines or produced during physiological activities, then secreting them as harmless substances into bile and blood for excretion. Liver disease is a common clinical condition. Causes of liver damage include surgical trauma, injury, microbial infection, liver disease itself, toxic compounds, drug side effects, immune disorders, genetic variations, metabolic abnormalities, and physical and chemical stimuli. It is one of the most common clinical diseases that threaten health. Long-term liver damage often leads to liver fibrosis, which can further induce cirrhosis and liver failure.

[0003] Classic liver injury models used in research include chemical, immunological, biological, and alcoholic liver injury models. Biological liver injury models, in particular, have stringent requirements for establishment, and the ease with which pathogens can infect researchers limits their application in studies. Immunological liver injury models induce an immune response to produce specific antibodies, leading to hepatocyte damage. Currently, chemical inducers are the most commonly used by researchers both domestically and internationally for establishing animal models of liver injury. CCl4, thioacetamide, and ethanol are the most common chemical injury inducers and are also commonly used standard models for evaluating the efficacy of hepatoprotective drugs.

[0004] Alanine aminotransferase (ALT) is released into the blood in large quantities during the acute phase of various viral hepatitis and during drug-induced hepatocellular necrosis. Therefore, ALT is an important indicator for diagnosing liver injury, a sensitive indicator of acute hepatocellular damage, and an important indicator for evaluating the efficacy of hepatoprotective drugs. Summary of the Invention

[0005] After multiple liver protection efficacy trials, a formula with outstanding liver protection effect was selected. After verification, its liver protection effect is better than that of traditional liver protection drug formulas, and it has a better protective effect against liver damage.

[0006] The purpose of this invention is to provide a drug composition for liver protection that is rationally formulated, simple to prepare, effective, and free of toxic side effects and drug residues, as well as its preparation method.

[0007] This invention is achieved through the following technical solution:

[0008] A traditional Chinese medicine composition for preventing and treating liver damage, characterized by being prepared by mixing the following components in parts by weight: Bupleurum chinense 10-14 parts, Lonicera japonica 12-18 parts, Astragalus membranaceus 15-30 parts, Polygonatum sibiricum 8-12 parts, Longan aril 8-12 parts, Glycyrrhiza uralensis 8-12 parts, Gardenia jasminoides (fried) 8-12 parts, Plantago asiatica 8-12 parts.

[0009] The optimal combination of the composition of the present invention is: 12 parts Bupleurum chinense, 15 parts Lonicera japonica, 25 parts Astragalus membranaceus, 10 parts Polygonatum sibiricum, 10 parts Longan aril, 10 parts Glycyrrhiza uralensis, 10 parts Gardenia jasminoides (fried), and 10 parts Plantago asiatica.

[0010] The traditional Chinese medicine composition preparation of the present invention is one of powder, granule, oral liquid, pill, capsule and tablet.

[0011] The preparation method of the traditional Chinese medicine composition includes the following steps: (1) weigh each medicinal material according to the weight parts; (2) crush the medicinal materials in the composition and sieve them to obtain the final product.

[0012] The preparation method of the traditional Chinese medicine composition includes the following steps: (1) weigh each medicinal material according to the weight parts; (2) decoct the medicinal material with water, filter the decoction, concentrate the filtrate, spray dry the extract powder, add the excipients for formulation to obtain granules.

[0013] The method for preparing the traditional Chinese medicine composition includes the following steps: (1) weigh each medicinal material according to the stated weight parts; (2) decoct the medicinal materials with water, filter the decoction, concentrate the filtrate to contain 0.1g / mL-1.0g / mL of raw medicinal material, centrifuge to remove impurities, and obtain the final product.

[0014] The pharmaceutical properties of each component of this invention are as follows:

[0015] Bupleurum: This product is the dried root of Bupleurum chinense or Bupleurum stenoptera, both belonging to the Apiaceae family. Based on their different characteristics, they are commonly known as "Northern Bupleurum" and "Southern Bupleurum." It is harvested in spring and autumn, with stems, leaves, and soil removed before drying. Its properties and channels are pungent, bitter, and slightly cold. It enters the liver, gallbladder, and lung channels. Its functions are to release exterior pathogens and harmonize interior pathogens, raise yang, and soothe the liver. It is used to treat colds with fever, alternating chills and fever, chronic diarrhea due to spleen deficiency, uterine prolapse, and rectal prolapse.

[0016] Honeysuckle: This product refers to the dried flower buds or newly opened flowers of *Lonicera japonica*, a plant in the Caprifoliaceae family. It is harvested in early summer before the flowers open and then dried. Its properties are sweet and cold. It enters the Lung, Heart, and Stomach meridians. Its functions are clearing heat and detoxifying, dispersing heat and wind. It is mainly used to treat febrile diseases, wind-heat colds, lung-heat coughs, sore throats, dysentery due to heat toxins, breast swelling and pain, carbuncles and boils. It is also used for fish enteritis and skin rot.

[0017] Astragalus: This product is the dried root of *Astragalus mongholicus* or *Astragalus membranaceus*, both belonging to the Fabaceae family. It is harvested in spring and autumn, with the fibrous roots and root head removed, and then sun-dried. Its properties and channels are sweet and slightly warm. It enters the Lung and Spleen channels. Its functions are to tonify Qi and raise Yang, strengthen Wei Qi and consolidate the exterior, promote diuresis and reduce swelling, promote pus drainage and detoxification, and promote wound healing and tissue regeneration. It is mainly used to treat deficiency of Lung and Spleen Qi, sinking of Middle Qi, spontaneous sweating due to exterior deficiency, edema due to Qi deficiency, and carbuncles and boils that are difficult to ulcerate and slow to heal.

[0018] Polygonatum: This product is the dried rhizome of Polygonatum yunnanense, Polygonatum sibiricum, or Polygonatum multiflorum, all belonging to the Liliaceae family. Depending on its shape, it is commonly known as "Large Polygonatum," "Chicken Head Polygonatum," or "Ginger-shaped Polygonatum." It is harvested in spring and autumn, the fibrous roots are removed, it is washed, briefly blanched in boiling water or steamed until thoroughly cooked, and then dried. Its properties are sweet and neutral. It enters the spleen, lung, and kidney meridians. Its functions are to replenish qi and nourish yin, strengthen the spleen, moisten the lungs, and benefit the kidneys. It is indicated for spleen and stomach weakness, fatigue, dry mouth and poor appetite, dry cough due to lung deficiency, insufficient essence and blood, and excessive water retention due to yin deficiency.

[0019] Longan pulp: The aril of the longan plant (Sapindaceae family). Its functions include nourishing the heart and spleen, replenishing blood, and calming the mind. It is used for qi and blood deficiency, palpitations, forgetfulness, insomnia, and anemia with chlorosis.

[0020] Licorice: This product is the dried root and rhizome of *Glycyrrhiza uralensis*, *Glycyrrhiza inflata*, or *Glycyrrhiza glabra*, all belonging to the legume family. It is harvested in spring and autumn, the fibrous roots are removed, and it is sun-dried. Its functions include tonifying the spleen and replenishing qi, resolving phlegm and relieving cough, harmonizing the stomach and relieving spasms, detoxifying, harmonizing other herbs, and mitigating the toxicity and harshness of other drugs. It is mainly used to treat spleen and stomach weakness, fatigue, cough and asthma, sore throat, poisoning, and sores.

[0021] Fried Gardenia: Take crushed gardenia fruit, place it in a pan and stir-fry over low heat until golden brown. Remove and let cool. This product is the dried, mature fruit of the gardenia plant (Rubiaceae family). It is used to treat feverish restlessness and insomnia, jaundice, gonorrhea, diabetes, red eyes, sore throat, hematemesis, epistaxis, bloody dysentery, hematuria, carbuncles and sores caused by heat toxins, and sprains and swelling.

[0022] Plantain seed: This product is the dried, mature seed of *Plantago asiatica* or *Plantago depressa*, plants of the Plantaginaceae family. The fruit stalks are harvested in summer and autumn when the seeds are mature, dried in the sun, and the seeds are extracted by rubbing to remove impurities. Its properties and channels are sweet and cold. It enters the liver, kidney, lung, and small intestine channels. Its functions are to clear heat and promote diuresis, eliminate dampness and relieve strangury, and improve eyesight. It is mainly used to treat hematuria due to heat, diarrhea, red and swollen eyes, edema, and retained placenta. Attached Figure Description

[0023] Figure 1 Liver of rats in the blank group

[0024] Figure 2 Liver of model group rats Detailed Implementation

[0025] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] The present invention will be further explained below with reference to specific embodiments.

[0027] Example 1: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 10 parts of Bupleurum chinense, 12 parts of Lonicera japonica, 20 parts of Astragalus membranaceus, 10 parts of Polygonatum sibiricum, 10 parts of Longan aril, 10 parts of Glycyrrhiza uralensis, 10 parts of stir-fried Gardenia jasminoides, and 10 parts of Plantago asiatica. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0028] Example 2: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 10 parts of Bupleurum chinense, 12 parts of Lonicera japonica, 15 parts of Astragalus membranaceus, 8 parts of Polygonatum sibiricum, 8 parts of Longan aril, 8 parts of Glycyrrhiza uralensis, 8 parts of stir-fried Gardenia jasminoides, and 8 parts of Plantago asiatica. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0029] Example 3: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 12 parts of Bupleurum chinense, 15 parts of Lonicera japonica, 25 parts of Astragalus membranaceus, 10 parts of Polygonatum sibiricum, 10 parts of Longan aril, 10 parts of Glycyrrhiza uralensis, 10 parts of stir-fried Gardenia jasminoides, and 10 parts of Plantago asiatica. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0030] Example 4: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 14 parts of Bupleurum chinense, 18 parts of Lonicera japonica, 30 parts of Astragalus membranaceus, 12 parts of Polygonatum sibiricum, 12 parts of Longan aril, 12 parts of Glycyrrhiza uralensis, 12 parts of stir-fried Gardenia jasminoides, and 12 parts of Plantago asiatica. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a crude herb content of 0.5 g / mL.

[0031] Example 5: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 7 parts Bupleurum chinense, 10 parts Lonicera japonica, 5 parts Astragalus membranaceus, 3 parts Polygonatum sibiricum, 7 parts Longan aril, 4 parts Glycyrrhiza uralensis, 6 parts stir-fried Gardenia jasminoides, and 5 parts Plantago asiatica. Decoction of the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0032] Example 6: Preparation of a traditional Chinese medicine composition for preventing and treating liver damage: Take 20 parts of Bupleurum chinense, 30 parts of Lonicera japonica, 100 parts of Astragalus membranaceus, 50 parts of Polygonatum sibiricum, 30 parts of Longan aril, 30 parts of Glycyrrhiza uralensis, 40 parts of stir-fried Gardenia jasminoides, and 30 parts of Plantago asiatica. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a crude herb content of 0.5 g / mL.

[0033] Example 7: Preparation of a reduced formula of traditional Chinese medicine composition for preventing and treating liver damage: Take 12 parts of Bupleurum chinense, 15 parts of Lonicera japonica, 10 parts of Polygonatum sibiricum, 10 parts of Longan aril, 10 parts of Glycyrrhiza uralensis, and 10 parts of Plantago asiatica. Decoction of the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0034] Example 8: Preparation of an alternative formula for a traditional Chinese medicine composition for preventing and treating liver damage: Take 12 parts chrysanthemum, 15 parts honeysuckle, 25 parts codonopsis, 10 parts polygonatum, 10 parts white peony root, 10 parts licorice root, 10 parts stir-fried gardenia, and 10 parts plantain seed. Decoct the herbs in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and adjust to a raw herb content of 0.5 g / mL.

[0035] Comparative Example 1: Extraction and preparation of the Longdan Xiegan San formula: Take 45 parts of Longdan (Gentiana scabra), 30 parts of Cheqianzi (Plantago asiatica), 30 parts of Chaihu (Bupleurum chinense), 30 parts of Danggui (Angelica sinensis), 30 parts of Zhizi (Gardenia jasminoides), 45 parts of Shengdihuang (Rehmannia glutinosa), 15 parts of Gancao (Glycyrrhiza uralensis), 30 parts of Huangqin (Scutellaria baicalensis), 45 parts of Zexie (Alisma plantago-aquatica), and 20 parts of Mutong (Akebia trifoliata). Decoction of the medicinal materials in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and prepare to a content of 0.5 g / mL of raw herbs.

[0036] Comparative Example 2: Extraction and preparation of Yin Chen Mu Tong San formula: Take 15g of Yin Chen, 15g of Lian Qiao, 12g of Jie Geng, 12g of Chuan Mu Tong, 18g of Cang Zhu, 12g of Chai Hu, 9g of Sheng Ma, 15g of Qing Pi, 15g of Chen Pi, 12g of Ze Lan, 9g of Jing Jie, 9g of Fang Feng, 15g of Bing Lang, 18g of Dang Gui, and 18g of Qian Niu Zi. Decoction of the medicinal materials in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and prepare to a raw drug content of 0.5g / mL.

[0037] Comparative Example 3: Extraction and preparation of the Hepatitis Recovery Pill formula: Take 75 parts of Artemisia capillaris, 75 parts of Curcuma longa, 75 parts of Isatis indigotica, 75 parts of Angelica sinensis, 75 parts of Chrysanthemum morifolium, 75 parts of Lysimachia christinae, 75 parts of Salvia miltiorrhiza, 75 parts of Talcum, and 75 parts of Polygonum bistorta. Decoction of the medicinal materials in water, filter the decoction, concentrate the filtrate, centrifuge to remove impurities, and prepare to a raw drug content of 0.5 g / mL.

[0038] Comparative Example 4: Extraction and preparation of the formula of Chaihu Shugan Wan (Bupleurum Liver-Soothing Pill). 100 parts of Poria cocos, 50 parts of stir-fried Citrus aurantium, 40 parts of Amomum villosum, 50 parts of white peony root (processed with wine), 50 parts of licorice root, 75 parts of vinegar-processed Cyperus rotundus, 50 parts of dried tangerine peel, 50 parts of Platycodon grandiflorus, 50 parts of ginger-processed Magnolia officinalis, 50 parts of stir-fried Crataegus pinnatifida, 50 parts of Saposhnikovia divaricata, 50 parts of stir-fried Shenqu (medicated leaven), 75 parts of Bupleurum chinense, 50 parts of Scutellaria baicalensis, 50 parts of Mentha haplocalyx, 75 parts of Perilla frutescens stem, 25 parts of Aucklandia lappa, 75 parts of stir-fried Areca catechu, 50 parts of vinegar-processed Sparganium stoloniferum, 50 parts of wine-processed Rheum palmatum, 50 parts of stir-fried Citrus reticulata peel, 50 parts of Angelica sinensis, 75 parts of ginger-processed Pinellia ternata, 50 parts of Lindera strychnifolia, and 50 parts of vinegar-processed Curcuma zedoaria. The medicinal materials were decocted in water, the decoction was filtered, the filtrate was concentrated, centrifuged to remove impurities, and the concentration was adjusted to 0.5 g / mL.

[0039] Test 1: Hepatoprotective tests of Example 1 and Comparative Examples 1-4 to determine the formulation with good hepatoprotective effect.

[0040] Test materials

[0041] Mouse cages; gavage needles; electronic scales; mouse holders; precision electronic balances; low-speed centrifuges; pipettes; automatic biochemical analyzers; laminar flow hoods; carbon tetrachloride (analytical grade), 500 mL / bottle, Beijing Chemical Reagent Factory; edible peanut oil.

[0042] Test animals

[0043] SD rats, SCXK(Beijing) 2016-0006, Beijing Vital River Laboratory Animal Technology Co., Ltd., male, 200 (±20) g, adaptively raised for more than 3 days before the experiment.

[0044] Test grouping

[0045] The test grouping is shown in Table 1

[0046] Table 1. Test grouping plan

[0047]

[0048] Test methods

[0049] Test grouping

[0050] The test rats are randomly divided into 7 groups: healthy control group, model control group, Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, with 10 rats in each group.

[0051] Modeling method

[0052] A rat liver fibrosis model is established. Carbon tetrachloride is formulated into a 40% solution with peanut oil. Except for the blank control group, the other groups are subcutaneously injected with 3 mL / kg of the 40% carbon tetrachloride solution in the back twice a week for 6 consecutive weeks. The control group rats are subcutaneously injected with 3 mL / kg of peanut oil of the same amount and frequency, and are fed with normal diet and water. Except for the healthy control group and the model control group that are gavaged with purified water, the other groups are given corresponding drugs for gavage to rats at a fixed time every day.

[0053] During the test process, the mental states of each group are observed, and finally the appearance, color, and texture of their livers are observed by autopsy; the serum alanine aminotransferase (ALT) is detected using an automatic biochemical analyzer.

[0054] Test results

[0055] Clinical symptom results

[0056] Before modeling, all rats were in good mental state, sensitive in response, with white, smooth, shiny and soft fur. During the experiment, all rats in the healthy control group survived until the end of the experiment, with good mental state, sensitive in response, and white, smooth, shiny and soft fur. The rats in the model control group showed restlessness and irritability in the early stage, with rough, sparse, dirty and dull fur. The autopsy results showed ( Figure 1 , 2 ), the liver surface of rats in the healthy control group was relatively smooth, the liver edge was relatively thin, its texture was relatively soft and showed a color ranging from red to dark red, with a shiny feeling, and there were no swelling and bleeding points. The liver surface of rats in the model control group was uneven compared with that of the normal group and showed obvious swelling, with more bleeding points in the center and at the edge, the color was lighter and close to loess color, the liver edge showed bluntness, the texture was harder, and some specimens showed nodular shape, indicating successful model establishment.

[0057] Serological test results

[0058] Table 2 Results of liver function indexes of rats in each group

[0059]

[0060] Note: Different letters indicate significant differences between groups

[0061] The test results showed that the liver protection effect of Example 1 was better than that of the commonly used existing liver protection drugs and could reduce the liver injury caused by carbon tetrachloride.

[0062] Test 2: Liver protection tests of Examples 2 - 8 to determine the formulation with good liver protection effect

[0063] Test materials

[0064] Mouse cages; gavage needles; electronic scales; mouse fixators; precision electronic balances; low-speed centrifuges; pipettors; automatic biochemical analyzers; laminar flow hoods; carbon tetrachloride (analytical pure), 500 mL / bottle, Beijing Chemical Reagent Factory; edible peanut oil

[0065] Test animals

[0066] SD rats (SCXK(Beijing)2016 - 0006, Beijing Vital River Laboratory Animal Technology Co., Ltd.), male, healthy, 200 (±20) g, and adaptively raised for more than 3 days before the experiment.

[0067] Test grouping

[0068] The test grouping is shown in Table 3

[0069] Table 3. Test grouping plan

[0070]

[0071] Test methods

[0072] Experimental grouping

[0073] The experimental rats were randomly divided into 9 groups: a healthy control group, a model control group, and Examples 2-8, with 10 rats in each group.

[0074] Modeling methods

[0075] A rat model of liver fibrosis was established by subcutaneously injecting 3 mL / kg of carbon tetrachloride solution into the back of rats using peanut oil to prepare a 40% solution. Except for the blank control group, all other groups received the same solution twice a week for 6 consecutive weeks. The control group rats received an equal amount of peanut oil (3 mL / kg) subcutaneously and were fed a normal diet and water. Except for the healthy control group and the model control group, which were given purified water by gavage, the other groups received the corresponding drugs by gavage at fixed times each day.

[0076] During the experiment, the mental state of each group was observed, and the shape, color, and texture of the liver were observed during the final autopsy. Serum alanine aminotransferase (ALT) was detected using a fully automated biochemical analyzer.

[0077] Test results

[0078] Clinical symptom results

[0079] Similar to the results of the above experiments, there were significant differences between the healthy control group and the model control group, indicating that the model was successfully established.

[0080] The serological test results are shown in Table 4.

[0081] Table 4. Liver function indicators of rats in each group

[0082]

[0083] Note: Different letters indicate that there are significant differences between groups.

[0084] The experimental results showed that Example 3 had the best liver-protective effect. However, when the formulation of this invention was not the recommended optimal combination, the efficacy of each group decreased. Reducing the formula resulted in a lower liver-protective effect, and replacing some herbs with similar herbs also reduced the overall efficacy. The optimal liver-protective combination was: Bupleurum chinense 12 parts, Lonicera japonica 15 parts, Astragalus membranaceus 25 parts, Polygonatum sibiricum 10 parts, Longan arillus 10 parts, Glycyrrhiza uralensis 10 parts, Gardenia jasminoides (fried) 10 parts, and Plantago asiatica 10 parts.

[0085] Compared with commonly used hepatoprotective drugs, the formulation of this invention has better effects and can reduce liver damage caused by carbon tetrachloride. Comparative experiments show that the formulation of this invention has excellent hepatoprotective effects.

[0086] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A traditional Chinese medicine composition for treating liver damage, characterized in that, It is prepared by mixing the following components in parts by weight: Bupleurum chinense 10-14 parts, Lonicera japonica 12-18 parts, Astragalus membranaceus 15-30 parts, Polygonatum sibiricum 8-12 parts, Longan aril 8-12 parts, Glycyrrhiza uralensis 8-12 parts, Gardenia jasminoides (fried) 8-12 parts, Plantago asiatica 8-12 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, It is prepared by mixing the following components in parts by weight: Bupleurum chinense 12 parts, Lonicera japonica 15 parts, Astragalus membranaceus 25 parts, Polygonatum sibiricum 10 parts, Longan aril 10 parts, Glycyrrhiza uralensis 10 parts, Gardenia jasminoides 10 parts, and Plantago asiatica 10 parts.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, It is prepared by mixing the following components in the indicated weight proportions: Bupleurum 10 parts, Honeysuckle 12 parts, Astragalus 20 parts, Polygonatum 10 parts, Longan pulp 10 parts, Licorice 10 parts, Fried Gardenia 10 parts, and Plantago seed 10 parts.

4. The traditional Chinese medicine composition according to any one of claims 1-3, characterized in that, Traditional Chinese medicine composition preparations include powders, granules, oral liquids, pills, capsules, and tablets.

5. The traditional Chinese medicine composition according to any one of claims 1-3, wherein the preparation method comprises the following steps: (1) Weigh each medicinal material according to the stated weight proportions; (2) Crush the medicinal materials in the prescription and sieve them to obtain the final product.

6. The traditional Chinese medicine composition according to any one of claims 1-3, wherein the preparation method comprises the following steps: (1) Weigh each medicinal material according to the stated weight proportions; (2) Boil the medicinal materials in water, filter the decoction, concentrate the filtrate, and spray dry it to obtain the extract powder. Add the excipients for formulation to obtain granules.

7. The traditional Chinese medicine composition according to any one of claims 1-3, wherein the preparation method comprises the following steps: (1) Weigh each medicinal material according to the stated weight proportions; (2) Boil the medicinal materials in water, filter the decoction, concentrate the filtrate to contain 0.1g / mL-1.0g / mL of raw medicinal materials, centrifuge to remove impurities, and the product is obtained.

8. The use of the traditional Chinese medicine composition according to any one of claims 1-7 in the preparation of a medicament for protecting the liver and reducing liver damage caused by carbon tetrachloride.

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