Application of a Chinese medicine composition in preparing medicine for treating nervous system diseases

Abstract

The invention provides an application of a Chinese medicine composition in the preparation of a drug for treating a nervous system disease, and relates to the field of pharmaceutical compositions, wherein the Chinese medicine composition comprises 40-60 parts of ginseng, 1-8 parts of pilose antler, 8-30 parts of astragalus, 8-30 parts of tortoise shell glue, 8-30 parts of prepared rehmannia root, 8-30 parts of cyathula, 8-30 parts of yam, 10-40 parts of salvia miltiorrhiza, 8-30 parts of wolfberry, 8-30 parts of dodder seed, 2-10 parts of schisandra chinensis, and 8-30 parts of parasitic loranthus; the nervous system disease is cognitive dysfunction. The invention expands the application direction of the Chinese medicine composition, and the Chinese medicine composition has a good therapeutic effect on nervous system diseases such as cognitive dysfunction.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical compositions, and in particular to application of a traditional Chinese medicine composition in the preparation of drugs for treating nervous system diseases. Background Art

[0002] The prescription of Guiqishen oral liquid is composed of ginseng, pilose antler, astragalus, tortoise shell glue, prepared rehmannia root, Achyranthes bidentata, Chinese yam, salvia miltiorrhiza, wolfberry, dodder seed, schisandra chinensis and parasitic loranthus, and has the effect of nourishing yin and tonifying yang. In addition to Guiqishen oral liquid, preparations made using the relevant raw materials of Guiqishen also include other dosage forms, such as dripping pills, pastes and powders. For example, patent CN100375608A also discloses Guiqishen dripping pills and a preparation method thereof. The dripping pills in the invention are prepared from extracts containing 12 active medicinal ingredients of Chinese medicines, including ginseng, pilose antler, astragalus, tortoise shell glue, prepared rehmannia root, Achyranthes bidentata, Chinese yam, salvia miltiorrhiza, wolfberry, dodder seed, schisandra chinensis and parasitic loranthus, and are prepared together with a pharmaceutically acceptable carrier as a matrix, and can be used for the treatment of stunted development, long-term illness weight, fatigue, insomnia and other symptoms. Another example is the book: Jiang Zhengguo, Zhang Wanchang. Chinese Wolfberry Application Collection [M]. Sunshine Publishing House, 2016: 63. There is a record of Guiqi Shen Gao, whose composition is 20g each of wolfberry and dodder seed, 50g ginseng, 2g deer antler, 20g each of astragalus, tortoise shell glue, prepared rehmannia, Achyranthes bidentata, and yam, 30g salvia miltiorrhiza, 6g schisandra chinensis, and 20g mistletoe.

[0003] At present, various preparations related to Guiqishen are often used to nourish qi and blood, nourish yin and assist yang. Some researchers have conducted research on other uses of Guiqishen preparations. For example, thesis: Deng Xiao. Clinical Observation on the Treatment of Chronic Fatigue Syndrome (Qi and Blood Deficiency Syndrome) with Guiqishen Oral Liquid [D]. Heilongjiang University of Chinese Medicine, 2010. The effectiveness and safety of Guiqishen Oral Liquid in the treatment of chronic fatigue syndrome (Qi and Blood Deficiency Syndrome) were observed through a randomized, double-blind, positive drug parallel control method.

[0004] Therefore, there is room for further expansion of the uses of Guiqishen, and it is very necessary to find new uses for this Chinese medicine composition. Summary of the invention

[0005] In view of the problems existing in the prior art, the present invention provides an application of a Chinese medicine composition in the preparation of a drug for treating nervous system diseases, thereby expanding the application direction of the Chinese medicine composition.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] The Chinese medicine composition provided by the present invention can be used for preparing medicines for treating nervous system diseases. The Chinese medicine composition comprises 40-60 parts of ginseng, 1-8 parts of pilose antler, 8-30 parts of astragalus, 8-30 parts of tortoise shell glue, 8-30 parts of prepared rehmannia, 8-30 parts of achyranthes, 8-30 parts of yam, 10-40 parts of salvia miltiorrhiza, 8-30 parts of wolfberry, 8-30 parts of dodder seed, 2-10 parts of schisandra chinensis and 8-30 parts of parasitic plant. The nervous system disease is cognitive dysfunction.

[0008] Preferably, the Chinese medicine composition comprises 45-55 parts of ginseng, 1-3 parts of pilose antler, 10-25 parts of astragalus, 10-25 parts of tortoise shell glue, 10-25 parts of rehmannia root, 10-25 parts of achyranthes bidentata, 10-25 parts of yam, 20-40 parts of salvia miltiorrhiza, 10-25 parts of wolfberry, 10-25 parts of dodder seed, 3-8 parts of schisandra chinensis and 10-25 parts of mistletoe.

[0009] Further preferably, by weight, it includes 50 parts of ginseng, 2 parts of pilose antler, 20 parts of astragalus, 20 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of Achyranthes bidentata, 20 parts of yam, 30 parts of salvia miltiorrhiza, 20 parts of wolfberry, 20 parts of dodder seed, 6 parts of schisandra chinensis and 20 parts of mistletoe.

[0010] Furthermore, the medicine also includes medically acceptable excipients.

[0011] Furthermore, the weight ratio of the velvet antler, tortoise shell glue and wolfberry is 1-3:10-25:10-25, and the weight ratio of the yam, dodder seed and mistletoe is 10-25:10-25:10-25.

[0012] Preferably, the weight ratio of the pilose antler, tortoise shell glue and wolfberry is 1:10:10.

[0013] Preferably, the weight ratio of yam, dodder seed and loranthus parasiticus is 1:1:1.

[0014] Furthermore, the dosage form of the drug includes oral liquid, powder, tablet, capsule, granule, paste, powder, spray or pill.

[0015] Preferably, the dosage form of the drug is an oral solution.

[0016] Furthermore, the preparation method of the oral liquid comprises the following steps:

[0017] (1) Extracting ginseng with alcohol, combining and concentrating the extracts, and then subjecting the extracts to alcohol precipitation, and concentrating the supernatant to obtain extract A;

[0018] (2) extracting the velvet antler with alcohol to obtain an extract A and a medicinal residue, decocting the medicinal residue with water to obtain a decoction, combining the decoction with the extract A, filtering, and concentrating to obtain an extract B;

[0019] (3) Add water to the tortoise shell glue and heat to melt it;

[0020] (4) Add water to the remaining medicinal materials in the formula and boil them, combine the decoctions, filter, concentrate, and precipitate with alcohol, take the supernatant, and concentrate to obtain extract C;

[0021] (5) Dissolve sucrose in water and heat to obtain monosaccharide syrup; dissolve sorbic acid in ethanol to obtain sorbic acid ethanol solution;

[0022] (6) Mix extract A, extract B, extract C, monosaccharide syrup and sorbic acid ethanol solution, add water and stir, let stand, filter, fill and sterilize to obtain the product.

[0023] In some specific embodiments, the method for preparing the oral liquid comprises the following steps:

[0024] (1) Ginseng was extracted three times with 60% ethanol by volume, heated under reflux, for 3 h in the first extraction and 2 h in the second and third extractions. The extracts were combined, the ethanol was recovered, and the extracts were concentrated to twice the weight of the original drug. Ethanol was added until the alcohol content was 80% (mass fraction). The extract was allowed to settle, the supernatant was taken, the ethanol was recovered, and the extract was concentrated to obtain extract A.

[0025] (2) Deer antler was extracted four times with 50% ethanol by volume, the first time for 3 h, the second and third times for 2 h, and the fourth time for 1 h. The extracts were combined to obtain extract A. The residue was decocted with water for 3-5 times. The decoction was combined with extract A, filtered, and ethanol was recovered from the filtrate. The filtrate was concentrated to a mass ratio of 1:1 with the original medicinal material. Ethanol was added to 85% (mass fraction), and the mixture was allowed to precipitate. The supernatant was taken, ethanol was recovered, and concentrated to obtain extract B.

[0026] (3) Add water to the tortoise shell glue and heat to melt it;

[0027] (4) The remaining medicinal materials in the formula were decocted three times with water, the first time for 3 h, the second and third times for 2 h each, the decoctions were combined, filtered, concentrated to twice the mass of the original medicinal materials, ethanol was added to 65% (mass fraction), allowed to settle, the supernatant was collected, and the ethanol was recovered to obtain extract C;

[0028] (5) Dissolve sucrose in water and heat to boil for 30 minutes to obtain monosaccharide syrup; dissolve sorbic acid in ethanol to obtain sorbic acid ethanol solution;

[0029] (6) Mix extract A, extract B, extract C, monosaccharide syrup and sorbic acid ethanol solution, add water and stir, let stand, filter, fill and sterilize to obtain the product.

[0030] Furthermore, when the dosage form of the drug is powder, tablet, capsule, granule, paste, powder, spray or pill, it can be prepared according to conventional methods in the art.

[0031] Raw materials in the present invention:

[0032] Ginseng: enters the spleen, lung, and heart meridians; sweet, slightly bitter, neutral; greatly replenishes vital energy, restores pulse and consolidates collapse, replenishes spleen and lungs, promotes body fluid, and calms the mind. Used for weakness and collapse, cold limbs and weak pulse, spleen deficiency and poor appetite, lung deficiency and wheezing and coughing, thirst due to loss of body fluid, internal heat and thirst, long-term illness and weakness, palpitations and insomnia, impotence and cold uterus; heart failure, cardiogenic shock.

[0033] Deer antler: enters the kidney and liver meridians; sweet, salty, warm; strengthens kidney yang, replenishes essence and blood, strengthens tendons and bones, regulates Chong and Ren meridians, and relieves sores and poisons. It is used for impotence and spermatorrhea, cold uterus and infertility, emaciation, fatigue, fear of cold, dizziness, tinnitus, deafness, cold pain in the waist and spine, weakness of tendons and bones, metrorrhagia and leukorrhea, and genital sores that do not heal.

[0034] Astragalus: enters the lung and spleen meridians; sweet, warm; replenishes qi and consolidates the exterior, promotes diuresis and expel toxins, discharges pus, heals sores and promotes tissue regeneration. Used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, chronic diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, edema due to qi deficiency, carbuncle that is difficult to heal, long-term sores that do not heal, blood deficiency and jaundice, internal heat and thirst; proteinuria in chronic nephritis, and diabetes.

[0035] Turtle Shell Glue: Enters the liver, kidney, and heart meridians; salty, sweet, and cool; nourishes yin, nourishes blood, and stops bleeding. Used for yin deficiency, hot flashes, bone steaming, night sweats, soreness of the waist and knees, blood deficiency, sallow complexion, and metrorrhagia and leucorrhea.

[0036] Rehmannia root: enters the liver and kidney meridians; sweet, slightly warm; nourishes yin and blood, replenishes essence and fills marrow. Used for liver and kidney yin deficiency, soreness of waist and knees, bone steaming and hot flashes, night sweats and spermatorrhea, internal heat and thirst, blood deficiency and sallow complexion, palpitations, irregular menstruation, metrorrhagia, dizziness, tinnitus, and premature graying of hair.

[0037] Achyranthes bidentata: enters the liver and kidney meridians; bitter, sour, neutral; nourishes the liver and kidneys, strengthens tendons and bones, removes blood stasis and dredges the meridians, and guides blood downward. Used for soreness of waist and knees, weakness of tendons and bones, amenorrhea, masses, and dizziness caused by liver yang.

[0038] Chinese Yam: Enters the spleen, lung, and kidney meridians; sweet, neutral; nourishes the spleen and stomach, promotes the production of body fluids and benefits the lungs, nourishes the kidneys and astringes semen. Used for spleen deficiency, poor appetite, chronic diarrhea, asthma and cough due to lung deficiency, spermatorrhea due to kidney deficiency, leucorrhea, frequent urination, and thirst due to deficiency heat. Chinese Yam Stir-fried with Bran Tonifies the Spleen and Strengthens the Stomach. Used for spleen deficiency, poor appetite, diarrhea, loose stools, and excessive leucorrhea.

[0039] Danshen: Root of Radix Salviae Miltiorrhizae ... bitter, slightly cold; removes blood stasis and relieves pain, activates blood circulation and dredges menstruation, clears the heart and relieves restlessness. Used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, stabbing pain in the chest and abdomen, pain caused by heat arthralgia, swelling and pain caused by sores, restlessness and insomnia; hepatosplenomegaly, angina pectoris.

[0040] Lycium barbarum: enters the liver and kidney meridians; sweet, neutral; nourishes the liver and kidneys, improves the essence and improves eyesight. Used for asthenia, loss of essence, soreness of waist and knees, dizziness and tinnitus, internal heat and thirst, blood deficiency and sallow complexion, blurred vision.

[0041] Cuscuta: enters the liver, kidney, and spleen meridians; sweet, warm; nourishes the liver and kidney, consolidates the essence and reduces urine, stabilizes the fetus, improves eyesight, and stops diarrhea. It is used for impotence and spermatorrhea, residual urine, enuresis and frequent urination, soreness of the waist and knees, blurred vision and tinnitus, kidney deficiency and fetal leakage, restless fetal movement, spleen and kidney deficiency diarrhea; external treatment of vitiligo.

[0042] Schisandra chinensis: enters the lung, heart and kidney meridians; sour, sweet and warm; astringent, replenishing qi and promoting body fluid, nourishing the kidney and calming the mind. Used for chronic cough, weak asthma, nocturnal emission, spermatorrhea, enuresis and frequent urination, chronic diarrhea, spontaneous sweating, night sweats, thirst due to loss of body fluid, shortness of breath and weak pulse, internal heat and thirst, palpitations and insomnia.

[0043] Mulberry parasite: enters the liver and kidney meridians; bitter, sweet, neutral; nourishes the liver and kidneys, strengthens the tendons and bones, removes rheumatism, and stabilizes the fetus. Used for rheumatic pain, soreness of the waist and knees, weakness of the tendons and bones, metrorrhagia, bleeding during pregnancy, restless fetuses, and hypertension.

[0044] The technical effects achieved by the present invention are:

[0045] The present invention provides a new use of a Chinese medicine composition in the preparation of medicines for treating nervous system diseases. The nervous system diseases are mainly cognitive dysfunctions. The combined use of 12 herbs including ginseng, pilose antler, astragalus, tortoise shell glue, rehmannia root, Achyranthes bidentata, yam, salvia miltiorrhiza, wolfberry, dodder seed, schisandra chinensis and mistletoe can effectively improve cognitive ability and alleviate cognitive dysfunction. DETAILED DESCRIPTION

[0046] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0047] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing specific embodiments rather than for limiting the scope of protection of the present invention.

[0048] When the embodiment gives a numerical range, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs.

[0049] It is worth noting that the raw materials used in the present invention are all common commercially available products, so their sources are not specifically limited.

[0050] Basic Example 1

[0051] A Chinese medicine composition:

[0052] The Chinese medicine composition comprises 40 parts of ginseng, 1 part of pilose antler, 8 parts of astragalus, 8 parts of tortoise shell glue, 8 parts of rehmannia root, 8 parts of Achyranthes bidentata, 8 parts of yam, 10 parts of salvia miltiorrhiza, 8 parts of wolfberry, 8 parts of dodder seed, 2 parts of schisandra chinensis and 8 parts of mistletoe.

[0053] Basic Example 2

[0054] A Chinese medicine composition:

[0055] The Chinese medicine composition is specifically formulated as follows by weight: 60 parts of ginseng, 8 parts of pilose antler, 30 parts of astragalus, 30 parts of tortoise shell glue, 30 parts of rehmannia root, 30 parts of Achyranthes bidentata, 30 parts of yam, 40 parts of salvia miltiorrhiza, 30 parts of wolfberry, 30 parts of dodder seed, 10 parts of schisandra chinensis and 30 parts of mistletoe.

[0056] Basic Example 3

[0057] A Chinese medicine composition:

[0058] The Chinese medicine composition comprises 45 parts of ginseng, 1 part of pilose antler, 10 parts of astragalus, 10 parts of tortoise shell glue, 10 parts of rehmannia root, 10 parts of Achyranthes bidentata, 10 parts of yam, 20 parts of salvia miltiorrhiza, 10 parts of wolfberry, 10 parts of dodder seed, 3 parts of schisandra chinensis and 10 parts of mistletoe.

[0059] Basic Example 4

[0060] A Chinese medicine composition:

[0061] The specific formula of the Chinese medicine composition is as follows by weight: 55 parts of ginseng, 3 parts of deer antler, 25 parts of astragalus, 25 parts of tortoise shell glue, 25 parts of rehmannia root, 25 parts of Achyranthes bidentata, 25 parts of yam, 40 parts of salvia miltiorrhiza, 25 parts of wolfberry, 25 parts of dodder seed, 8 parts of schisandra chinensis and 25 parts of mistletoe.

[0062] Basic Example 5

[0063] A Chinese medicine composition:

[0064] The Chinese medicine composition comprises 50 parts of ginseng, 2 parts of pilose antler, 20 parts of astragalus, 20 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of Achyranthes bidentata, 20 parts of yam, 30 parts of salvia miltiorrhiza, 20 parts of wolfberry, 20 parts of dodder seed, 6 parts of schisandra chinensis and 20 parts of mistletoe.

[0065] Application Example 1

[0066] Guiqishen oral liquid is prepared using the formula of basic embodiment 1, and the preparation method is specifically as follows:

[0067] (1) Ginseng was extracted three times with 60% ethanol by volume, heated under reflux, for 3 h in the first extraction and 2 h in the second and third extractions. The extracts were combined, the ethanol was recovered, and the extracts were concentrated to twice the weight of the original drug. Ethanol was added until the alcohol content was 80% (mass fraction). The extract was allowed to settle, the supernatant was taken, the ethanol was recovered, and the extract was concentrated to obtain extract A.

[0068] (2) Deer antler was extracted four times with 50% ethanol by volume, the first time for 3 h, the second and third times for 2 h, and the fourth time for 1 h. The extracts were combined to obtain extract A. The residue was decocted with water for 3-5 times. The decoction was combined with extract A, filtered, and ethanol was recovered from the filtrate. The filtrate was concentrated to a mass ratio of 1:1 with the original medicinal material. Ethanol was added to 85% (mass fraction), and the mixture was allowed to precipitate. The supernatant was taken, ethanol was recovered, and concentrated to obtain extract B.

[0069] (3) Add water to the tortoise shell glue and heat to melt it;

[0070] (4) The remaining medicinal materials in the formula were decocted three times with water, the first time for 3 h, the second and third times for 2 h each, the decoctions were combined, filtered, concentrated to twice the mass of the original medicinal materials, ethanol was added to 65% (mass fraction), allowed to settle, the supernatant was collected, and the ethanol was recovered to obtain extract C;

[0071] (5) Dissolve sucrose in water and heat to boil for 30 minutes to obtain monosaccharide syrup; dissolve sorbic acid in ethanol to obtain sorbic acid ethanol solution;

[0072] (6) Mix extract A, extract B, extract C, monosaccharide syrup and sorbic acid ethanol solution, add water and stir, let stand, filter, fill and sterilize to obtain the product.

[0073] Application Example 2

[0074] Guiqishen oral liquid is prepared using the formula of Basic Example 2, and the preparation method is the same as that of Application Example 2.

[0075] Application Example 3

[0076] Guiqishen oral liquid is prepared using the formula of Basic Example 3, and the preparation method is the same as that of Application Example 3.

[0077] Application Example 4

[0078] Guiqishen oral liquid is prepared using the formula of Basic Example 4, and the preparation method is the same as that of Application Example 4.

[0079] Application Example 5

[0080] Guiqishen oral liquid is prepared using the formula of Basic Example 5, and the preparation method is the same as that of Application Example 5.

[0081] Comparative Example 1

[0082] The only difference from Example 5 is that pilose antler and Achyranthes bidentata are not added to the formula.

[0083] That is, the composition is calculated by weight, and the specific formula is: 50 parts of ginseng, 20 parts of astragalus, 20 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of yam, 30 parts of salvia miltiorrhiza, 20 parts of wolfberry, 20 parts of dodder seed, 6 parts of schisandra chinensis and 20 parts of mistletoe.

[0084] The oral liquid was prepared according to the method of Application Example 5, except that pilose antler and Achyranthes bidentata were not added during the preparation.

[0085] Comparative Example 2

[0086] The only difference from Example 5 is that the ratio of pilose antler, tortoise shell glue and wolfberry in the formula is different (the total amount is the same).

[0087] That is, the composition is calculated by weight, and the specific formula is: 50 parts of ginseng, 5 parts of pilose antler, 20 parts of astragalus, 8 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of Achyranthes bidentata, 20 parts of yam, 30 parts of salvia miltiorrhiza, 29 parts of wolfberry, 20 parts of dodder seed, 6 parts of schisandra chinensis and 20 parts of mistletoe.

[0088] The oral liquid was prepared according to the method of Application Example 5, except that the amounts of some raw materials used in the preparation were different.

[0089] Comparative Example 3

[0090] The only difference from Example 5 is that the ratio of yam, dodder seed and mistletoe in the formula is different (the total amount is the same).

[0091] That is, the composition is calculated by weight, and the specific formula is: 50 parts of ginseng, 2 parts of pilose antler, 20 parts of astragalus, 20 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of Achyranthes bidentata, 28 parts of yam, 30 parts of salvia miltiorrhiza, 20 parts of wolfberry, 4 parts of dodder seed, 6 parts of schisandra chinensis and 28 parts of mistletoe.

[0092] The oral liquid was prepared according to the method of Application Example 5, except that the amounts of some raw materials used in the preparation were different.

[0093] Test example

[0094] 1. Experimental Animals:

[0095] SPF Wistar rats, half male and half female, with female rats weighing 200±25g and male rats weighing 325±25g, were provided by Beijing Huafukang Biotechnology Co., Ltd.

[0096] 2. Modeling and grouping:

[0097] Wistar rats were adaptively fed in an SPF environment for one week, 10 rats were selected as a blank control group and fed normally, and the remaining rats were modeled for cognitive impairment, specifically using 120 mg / kg of aluminum citrate combined with 30 mg / kg of sodium nitrite for intraperitoneal injection, and the administration was continued every other day for 30 days. The modeled rats were randomly divided into 9 groups, each with 10 rats (5 females and 5 males), of which 8 groups were respectively used as the oral liquid prepared by the application examples 1-5 groups and the comparative examples 1-3 groups, and the oral liquid of each group was administered by gavage, and the gavage volume was 1 mL / kg body weight / time, and the gavage was 2 times a day. The model control group and the blank control group rats were given an equal amount of normal saline. After 21 days, the rats were subjected to open field tests and Morris water maze tests, and were fed normally during the experiment.

[0098] 3. Experimental methods:

[0099] Experiment 1: Open field test

[0100] The experiment was conducted in a test box. The open field was divided into 16 squares of equal size, 4 of which were central areas and 12 were peripheral areas. Rats were placed in the central area for testing. An infrared open field test system was used to detect the number of square crossings by rats within 5 minutes, which is recorded in Table 1.

[0101] Experiment 2: Morris water maze test

[0102] A circular bucket (140 cm in diameter × 50 cm in height) was selected, and the pool was divided into four quadrants by two mutually perpendicular diameters of the bucket. A circular platform (7 cm in diameter) was set in the center of the second quadrant. The platform was 1-2 cm lower than the sleeping position, the water temperature was maintained at 20-22 °C, and the water depth was 40 cm. Five days before the experiment, the rats were trained four times a day. Each rat entered the pool from a different quadrant each time. Specifically, the rat was placed from the center point of the edge of the quadrant, facing the pool wall, and the rat was allowed to find the platform independently. The rats that found the platform within 60 seconds were recorded as the rat escape latency. For rats that did not find the platform within 60 seconds, tools were used to guide the rats to find the platform and stay on the platform for 10 seconds, and 60 seconds was recorded as the rat escape latency. After the training, the platform was removed, and the rats were placed in the pool using the training method. The rats were tracked for 60 seconds to explore the pool trajectory, and the time the rats spent in the quadrant where the original platform was located and the total time were recorded. The ratio of the two was calculated, and the results were statistically reported in Table 2.

[0103] 4. Experimental results:

[0104] Experiment 1:

[0105] Table 1 Number of times rats in each group passed through the grid

[0106]

[0107] (Note: Compared with the blank control group, # p<0.05; compared with the model control group, a p<0.05; compared with application example 5, b p<0.05. )

[0108] According to the results of the above table, the Guiqishen oral liquid prepared in each application example of the present invention can effectively increase the number of rats' traverse times, showing a significant difference compared with the model control group (p < 0.05). In contrast, although the number of rats' traverse times in Comparative Examples 1-3 has increased, the effect of the improvement is limited, and there is a significant difference between the effect of Application Example 1 (p < 0.05). In particular, the Comparative Example 1 group has no significant difference compared with the model control group, indicating that the Chinese medicine composition of the specific formula of the present invention has the corresponding effect.

[0109] Experiment 2:

[0110] Table 2 Escape latency and activity time in the quadrant where the original platform was located in each group of rats

[0111]

[0112] (Note: Compared with the blank control group, # p<0.05; compared with the model control group, a p<0.05; compared with application example 5, b p<0.05. )

[0113] According to the results in the table above, the Guiqishen oral liquid prepared in each application example of the present invention can effectively reduce the escape latency of rats and increase the quadrant time of the original platform, showing significant differences compared with the model control group (p < 0.05). Based on the above results, it can be seen that the composition of the present invention can effectively improve cognitive ability and alleviate cognitive impairment.

[0114] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. Use of a Chinese medicine composition in the preparation of a drug for treating a nervous system disease, characterized in that: The Chinese medicine composition is prepared from the following raw materials by weight: 40-60 parts of ginseng, 1-8 parts of pilose antler, 8-30 parts of astragalus, 8-30 parts of tortoise shell glue, 8-30 parts of rehmannia root, 8-30 parts of cyathula, 8-30 parts of yam, 10-40 parts of salvia miltiorrhiza, 8-30 parts of wolfberry, 8-30 parts of dodder seed, 2-10 parts of schisandra chinensis and 8-30 parts of mistletoe; The weight ratio of the velvet antler, tortoise shell glue and wolfberry is 1-3:10-25:10-25, and the weight ratio of the yam, dodder seed and mistletoe is 10-25:10-25:10-25; The nervous system disease is cognitive dysfunction, and the cause of the cognitive dysfunction is Alzheimer's disease.

2. The use according to claim 1, characterized in that: The Chinese medicine composition is made of the following raw materials by weight: 45-55 parts of ginseng, 1-3 parts of pilose antler, 10-25 parts of astragalus, 10-25 parts of tortoise shell glue, 10-25 parts of rehmannia root, 10-25 parts of achyranthes bidentata, 10-25 parts of yam, 20-40 parts of salvia miltiorrhiza, 10-25 parts of wolfberry, 10-25 parts of dodder seed, 3-8 parts of schisandra chinensis and 10-25 parts of mistletoe.

3. The use according to claim 2, characterized in that: The Chinese medicine composition is made of the following raw materials by weight: 50 parts of ginseng, 2 parts of pilose antler, 20 parts of astragalus, 20 parts of tortoise shell glue, 20 parts of rehmannia root, 20 parts of achyranthes bidentata, 20 parts of yam, 30 parts of salvia miltiorrhiza, 20 parts of wolfberry, 20 parts of dodder seed, 6 parts of schisandra chinensis and 20 parts of mistletoe.

4. The use according to claim 1, characterized in that: The weight ratio of the pilose antler, tortoise shell glue and wolfberry is 1:10:

10.

5. The use according to claim 1, characterized in that: The weight ratio of the Chinese yam, dodder seed and mistletoe is 1:1:

1.

6. The use according to claim 1, characterized in that: The dosage form of the drug includes oral liquid, powder, tablet, capsule, granule, paste, powder, spray or pill.

7. The use according to claim 6, characterized in that: The dosage form of the medicine is oral solution.

8. The use according to claim 7, characterized in that: The preparation method of the oral liquid comprises the following steps: (1) Extracting ginseng with alcohol, combining and concentrating the extracts, and then subjecting the extracts to alcohol precipitation, and concentrating the supernatant to obtain extract A; (2) extracting the velvet antler with alcohol to obtain an extract A and a medicinal residue, decocting the medicinal residue with water to obtain a decoction, combining the decoction with the extract A, filtering, and concentrating to obtain an extract B; (3) Add water to the tortoise shell glue and heat to melt it; (4) Add water to the remaining medicinal materials in the formula and boil them, combine the decoctions, filter, concentrate, and precipitate with alcohol, take the supernatant, and concentrate to obtain extract C; (5) Dissolve sucrose in water and heat to obtain monosaccharide syrup; dissolve sorbic acid in ethanol to obtain sorbic acid ethanol solution; (6) Mix extract A, extract B, extract C, monosaccharide syrup and sorbic acid ethanol solution, add water and stir, let stand, filter, fill and sterilize to obtain the product.

Images