Animal experiment skin round wound expansion fixer based on aperture structure

Abstract

The present application belongs to the technical field of animal skin wound experimental apparatus, and provides an animal experimental skin circular wound expansion fixer based on an aperture structure, which is mainly composed of a wound fixing base, expansion unit blades, a rotating mechanism cover plate and a spring component combination. When multiple expansion unit blades are limited by the leaf limit sliding groove on the wound fixing base through the limiting protrusions, the multiple expansion unit blades can form different sizes of combined central holes. When the rotating mechanism cover plate drives the expansion unit blades, the rotating mechanism cover plate rotates relative to the wound fixing base, and the rotation of the rotating mechanism cover plate on the wound fixing base is controlled through the spring stress. The fixer is designed based on the multi-point directional movement of the aperture structure, can realize the application of multiple directional fixing tension on the skin around the circular full-thickness wound in animal experiments, and is especially suitable for application in the related experimental research on the skin fibrosis induced in the wound healing process of experimental animals.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal skin wound experimental apparatus, and relates to an animal experimental skin circular wound expansion fixer based on an aperture structure, in particular to a skin circular wound expansion fixer based on an aperture structure suitable for circular full-thickness wounds in experimental animal modeling and an application method thereof. BACKGROUND

[0002] In the field of modern medical research, animal experiments, as a core method, are widely used to simulate and explore human physiological and pathological states. In particular, in the study of skin wound healing process, although animal models provide valuable experimental platforms, physiological differences between different species lead to different healing mechanisms, which to some extent brings great disturbance to the simulation of human skin healing process in physiological or pathological state. Therefore, how to accurately simulate the human skin wound healing process in physiological and pathological states, especially to simulate the pathological mechanical signal microenvironment of human body and induce the pathological persistent proliferation of wound tissue, has become an important problem that needs to be solved in the current scientific research field.

[0003] In animal experiments simulating human skin physiological healing, the silicone splint fixation method has been widely used. Specifically, a full-thickness incision is made on the skin of experimental animals (such as the back of mice or rats): a biopsy punch is used to cut and punch the skin tissue, and auxiliary forceps and ophthalmic scissors are used to cut and separate the adherent skin to form a circular full-thickness wound (including excision of panniculus carnosus). Then, a cyanoacrylate adhesive is used to paste a silicone splint on the skin around the wound, and a suture is used to further tightly fix the splint. This technique effectively reduces the promotion of skin contraction on the healing process, making the healing pattern of the animal model more similar to the physiological process of human skin healing.

[0004] However, although the silicone splint fixation method has achieved remarkable results in simulating human skin physiological healing, it has failed to successfully induce the formation of experimental animal skin fibrosis and hypertrophic scar. These two pathological phenomena are common in human wound healing process and are of great significance for in-depth understanding of wound healing mechanism.

[0005] To overcome this limitation, researchers have proposed two strategies: drug induction and mechanical stress induction. Among them, drug induction mainly simulates local inflammatory response and excessive deposition of collagen fibers by injecting bleomycin, which can simulate the formation of hypertrophic scar to some extent, but does not completely simulate the natural process of human skin fibrosis. In contrast, mechanical stress induction is considered a more potential method, which is based on the theory of simulating the mechanical environment in the human body to induce the occurrence of skin fibrosis, thereby more accurately simulating the pathological process of human skin healing.

[0006] However, the current technology related to mechanical stress-induced excessive proliferation of scar tissue (hypertrophic scar) is still in the exploratory stage. Some researchers choose to use existing fixation devices in the fields of oral medicine or surgery, but such fixation devices have many shortcomings that do not match the experiment in the physiological healing simulation experiment of skin wounds, such as difficult device fixation and complicated operation steps. In order to simplify the clinical operation as much as possible, the current wound expansion fixator for animal experiments related to mechanical stress-induced animal experiments is mostly a two-way wound expansion fixator that refers to existing surgical fixation devices, i.e., applying mutual vertical horizontal and vertical tensile forces to the skin around the full-thickness wound. For example, the applicant's prior granted invention patent "Animal experiment skin wound constant force expansion fixator and application thereof (202311341876.8)", but such wound expansion fixator has a single direction of force, which cannot completely simulate the actual tensile force situation of multiple directions from the real patient's skin full-thickness wound. In addition, single-direction expansion of the wound will cause the healing tissue to elongate in a single direction of tension, while the direction not subjected to tension will further shrink, ultimately leading to irregular and excessively long shape of the scar tissue, which is not conducive to subsequent analysis and processing of the scar tissue. In addition, excessive stretching of the scar in a single direction causes excessive deformation of the skin and wound, which causes discomfort to the animal and raises potential ethical issues. SUMMARY

[0007] The present application is to solve the above-mentioned problems in the prior art, and provides an animal experiment skin circular wound expansion fixator based on an aperture structure. The fixator is designed based on the multi-point directional movement of the aperture structure, which can apply multiple directional fixed tensile forces to the skin around the circular full-thickness wound in animal experiments, and at the same time, it is convenient to adjust and adapt to different sizes of circular full-thickness wounds in animal modeling experiments, especially suitable for application in experimental animal-induced skin fibrosis during wound healing related experimental research.

[0008] To achieve the above-mentioned purposes, the present application is realized by the following technical measures.

[0009] The present application provides an animal experiment skin circular wound expansion fixator based on an aperture structure, which is mainly composed of a wound fixation base, an expansion unit blade, a rotating mechanism cover plate and a spring combination,

[0010] The wound fixation base is a circular ring structure with a circular opening in the middle, and a plurality of blade limiting sliding grooves are arranged around the circular opening;

[0011] The expansion unit blade is provided with a limiting protrusion corresponding to the blade limiting sliding groove, when multiple expansion unit blades are limited by the limiting protrusion and move in the blade limiting sliding groove on the wound fixing base, the multiple expansion unit blades can form a combined central hole with different sizes; the expansion unit blade is provided with a suture hole at one end close to the combined central hole, and the expansion unit blade is also provided with a blade driving sliding groove;

[0012] The rotating mechanism cover plate is a circular ring structure with a circular opening in the middle, and is provided with multiple driving protrusions corresponding to the blade driving sliding groove around the circular opening, and the expansion unit blade is driven by the movement of the driving protrusions in the blade driving sliding groove when the rotating mechanism cover plate is rotated;

[0013] When the expansion unit blade is driven, the rotating mechanism cover plate rotates relative to the wound fixing base;

[0014] The wound fixing base and the rotating mechanism cover plate are respectively provided with a spring stress female part and a spring stress male part, and the two ends of the spring part are respectively installed on the spring stress female part and the spring stress male part, so that the spring stress separates or approaches the spring stress female part and the spring stress male part, and then the spring stress controls the rotation of the rotating mechanism cover plate on the wound fixing base.

[0015] The application point of the application is that the structure of the light ring can adjust the aperture size, and the multi-point directional movement design of the expansion unit blade can apply multiple directional fixing forces to the skin around the circular full-thickness wound in animal experiments, and can also conveniently adjust and adapt to different sizes of circular full-thickness wounds in animal modeling experiments. The application of multiple directional fixing forces by multiple expansion unit blades can ensure the invariability of the shape of the circular full-thickness wound and the uniformity of the tension on different parts of the wound, and the force on the wound tissue comes from all directions, not from a single direction, which can more truly simulate the mechanical stress state of human skin wound healing. In addition, the device is small and can be easily fixed on the surface of the experimental animal skin without being easily damaged or falling off; at the same time, the device can be adjusted and adapted to different sizes of circular full-thickness wounds, effectively avoiding the waste of materials and experimental animals. In summary, the design solves the shortcomings of the existing one-way expansion modeling scheme, can more truly simulate the stress state in human skin healing, and improves the modeling efficiency and success rate.

[0016] In addition, the application can also inhibit the contraction of the experimental animal meat membrane and reduce the promotion of wound healing. In animal experiment clinic, it can directly replace the conventional wound fixing device to simulate human physiological state wound healing; it can also inhibit the contraction of the meat membrane and pull the wound when modeling in pathological state, without the need to replace the device in the middle.

[0017] In one of the technical solutions, in order to better realize the rotation of the rotating mechanism cover plate relative to the wound fixation base, a rotation limiting column is arranged on the wound fixation base, and a rotation limiting rail matched with the rotation limiting column is arranged on the rotating mechanism cover plate. Further, the rotation limiting column has a protruding part that acts as a buckle, and the protruding part is buckled into the rotation limiting rail to realize the relative rotation fixation between the rotating mechanism cover plate and the wound fixation base.

[0018] In one of the technical solutions, in order to further stabilize and fix the full-thickness circular wound, a plurality of outer suture holes are further formed on the wound fixation base.

[0019] In this context, the expansion unit blade is a conventional aperture structure blade shape in the prior art, and the simultaneous movement of multiple expansion unit blades realizes the gradual change of the combined central hole from large to small or from small to large.

[0020] In one of the technical solutions, in order to realize the straight-line movement of the expansion unit blade in multiple-point directional movement, the blade limiting sliding groove and the blade driving sliding groove are straight-line grooves, and the expansion unit blade is a blade shape based on the aperture structure principle in the prior art and can move linearly.

[0021] In this context, the spring member is a conventional spring member with spring stress, and those skilled in the art can directly use a conventional spring to realize the separation or close of the spring stress parent member and the spring stress sub-member, and further preferably adopt a constant force spring to apply constant spring stress to both while realizing the separation or close of the spring stress parent member and the spring stress sub-member.

[0022] It should be noted that a constant force spring is a special spring that applies a constant force within its range of motion under theoretical conditions. When a constant force spring is used, its specific manner can refer to the prior invention patent "Animal Experiment Skin Wound Constant Force Expansion Fixer and Its Application (202311341876.8)" of the applicant of the present application.

[0023] In one of the technical solutions, in order to more conveniently install the ordinary spring, the spring stress parent member and the spring stress sub-member are both cylindrical shaft members that can rotate relative to their fixed positions, and a spring mounting shaft is fixedly installed on the spring stress sub-member, the other end of the spring mounting shaft penetrates the spring stress parent member, so that the spring stress parent member and the spring stress sub-member can move relative to each other, and the spring member is installed on the spring mounting shaft.

[0024] In one of the technical solutions, in order to isolate air as much as possible, the skin circular wound expansion fixator further comprises a transparent observation cover plate, which covers the rotating mechanism cover plate and completely covers the circular hole in the middle of the rotating mechanism cover plate.

[0025] In one of the technical solutions, in order to facilitate better recording of full-thickness wounds in animal experiments, scale lines are further provided on the transparent observation cover plate.

[0026] On the other hand, the application also provides an application method of the above-mentioned skin circular wound expansion fixator for animal experiments based on an aperture structure, mainly comprising the following steps:

[0027] (1) cutting and punching the skin tissue of the experimental animal, cutting and separating the adherent skin to form a circular full-thickness wound;

[0028] (2) pasting the wound fixation base of the skin circular wound expansion fixator on the skin around the full-thickness wound with an adhesive, so that the full-thickness wound is located in the circular hole of the wound fixation base, and the combined central hole is formed above the full-thickness wound by driving the expansion unit blade of the rotating mechanism cover plate, the size of the combined central hole matches the size of the full-thickness wound, and then the expansion unit blade and the skin around the full-thickness wound are fixed by suture through the suture hole;

[0029] (3) installing a spring member, using spring stress to separate or approach the spring stress parent member and the spring stress child member, and then controlling the rotation of the rotating mechanism cover plate on the wound fixation base through the spring stress, so as to exert traction force on the skin around the full-thickness wound in multiple different directions away from the wound.

[0030] In one of the technical solutions, when the experimental animal simulates the normal healing process of human beings and eliminates the influence of the experimental animal's fascia on wound healing, only the spring needs to be disassembled.

[0031] The application has the following beneficial effects:

[0032] 1、The skin circular wound expansion fixator for animal experiments based on an aperture structure provided by the application can exert multiple directional fixing forces on the skin around the circular full-thickness wound in animal experiments based on the multi-point directional movement design of the aperture structure, and can be easily adjusted to adapt to circular full-thickness wounds of different sizes in animal modeling experiments, and does not need to be disassembled and replaced after installation during the entire animal experiment period, thereby significantly reducing the complexity and labor cost of related experiments.

[0033] 2. The circular skin wound expansion and fixation device for animal experiments based on an aperture structure provided by the present invention allows for convenient replacement of the spring component during animal experiments according to different situations, thereby achieving different traction forces or constant force characteristics. It is particularly suitable for experimental studies on the formation of skin fibrosis in experimental animals during wound healing.

[0034] 3. The circular skin wound expansion and fixation device for animal experiments based on an aperture structure provided by the present invention, in one technical solution, by setting a transparent observation cover, can effectively reduce the influence of air on the wound without affecting observation. At the same time, the scale lines set on the transparent observation cover facilitate further recording and study of wound changes.

[0035] Instruction manual illustrations

[0036] Figure 1 This is a schematic diagram of the overall structure of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure, according to Embodiment 1 of the present invention.

[0037] Figure 2 This is an exploded view of the first state of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure, according to Embodiment 1 of the present invention.

[0038] Figure 3 This is an exploded view from another perspective of the first state of an animal experimental circular wound expansion and fixation device based on an aperture structure according to Embodiment 1 of the present invention.

[0039] Figure 4 This is an exploded view of the second state of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure, according to Embodiment 1 of the present invention.

[0040] Figure 5 This is a top-view structural diagram of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure, in its first state with the transparent observation cover removed. In the diagram, when the rotating mechanism cover rotates in the direction indicated by arrow A, the drive expansion unit blades change from the first state to the second state.

[0041] Figure 6 This is a top-view structural diagram of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure in Embodiment 1 of the present invention, with the transparent observation cover removed in the second state.

[0042] Figure 7 This is a schematic diagram of the bottom structure of multiple expansion unit blades in the second state of an animal experimental skin circular wound expansion and fixation device based on an aperture structure according to Embodiment 1 of the present invention.

[0043] Figure 8This is a schematic diagram of the bottom structure of the rotating mechanism cover plate in an animal experimental circular wound expansion and fixation device based on an aperture structure according to Embodiment 1 of the present invention.

[0044] Figure 9 This is a schematic diagram of the transparent observation cover plate in an animal experimental circular wound expansion and fixation device based on an aperture structure, according to Embodiment 1 of the present invention.

[0045] In the figure, 1 is the transparent observation cover, 2 is the rotating mechanism cover, 3 is the spring stress sub-component, 4 is the spring stress mother component, 5 is the spring component, 6 is the expansion unit blade, 7 is the wound fixation base, 8 is the limiting protrusion, 9 is the blade limiting slide groove, 10 is the rotating limiting post, 11 is the external suture hole, 12 is the driving protrusion, 13 is the spring mounting shaft, 14 is the suture hole, 15 is the blade driving slide groove, and 16 is the rotating limiting rail. Detailed Implementation

[0046] To further understand the present invention, preferred embodiments are described below in conjunction with examples. However, it should be understood that these descriptions are merely for further illustrating the features and advantages of the invention, and not for limiting the scope of the claims. Those skilled in the art can refer to the content of this document to appropriately improve the process parameters. It is particularly important to note that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included within the scope of this invention. The structure and application of the present invention have been described through preferred embodiments. Those skilled in the art can obviously make modifications or appropriate alterations and combinations to the structure and application described herein without departing from the content, spirit, and scope of the present invention to realize and apply the technology of the present invention. While it is believed that those skilled in the art will fully understand the following terms, the following definitions are stated to help illustrate the subject matter disclosed in this invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are followed. This application should not be construed as being limited to the specific embodiments described.

[0047] Example 1

[0048] like Figures 1-9 As shown in the figure, this embodiment of a circular skin wound expansion and fixation device for animal experiments based on an aperture structure is composed of a wound fixation base 7, an expansion unit blade 6, a rotating mechanism cover plate 2, a transparent observation cover plate 1, and a spring component 5.

[0049] The wound fixation base 7 is a ring structure with a circular opening in the middle, and multiple leaf limiting grooves 9 are provided around the circular opening; multiple external suture holes 11 are also provided on the wound fixation base 7.

[0050] The expansion unit blade 6 is provided with a limiting protrusion 8 corresponding to the blade limiting sliding groove 9, when the plurality of expansion unit blades 6 are limited by the limiting protrusion 8 and move in the blade limiting sliding groove 9 on the wound surface fixing base, the plurality of expansion unit blades 6 can form a combined central hole with different sizes; the expansion unit blade 6 is provided with a suture hole 14 at one end close to the combined central hole, and the blade driving sliding groove 15 is also provided on the expansion unit blade 6, and the blade limiting sliding groove 9 and the blade driving sliding groove 15 are straight grooves;

[0051] The rotating mechanism cover plate 2 is a circular ring structure with a circular opening in the middle, and is provided with a plurality of driving protrusions 12 corresponding to the blade driving sliding groove 15 around the circular opening, and the driving protrusions 12 are driven to move in the blade driving sliding groove 15 when the rotating mechanism cover plate 2 is rotated, thereby driving the expansion unit blade 6;

[0052] When the expansion unit blade 6 is driven, the rotating mechanism cover plate 2 rotates relative to the wound surface fixing base 7;

[0053] The wound surface fixing base 7 and the rotating mechanism cover plate 2 are respectively provided with a spring stress female part 4 and a spring stress male part 3, the spring stress female part 4 and the spring stress male part 3 are cylindrical shafts that can rotate relative to their fixed positions, and a spring mounting shaft 13 is fixedly installed on the spring stress male part 3, the other end of the spring mounting shaft 13 penetrates the spring stress female part 4 and moves, so that the spring stress female part 4 and the spring stress male part 3 can move relative to each other, the spring 5 is installed on the spring mounting shaft 13, so that the two ends of the spring 5 can act on the spring stress female part 4 and the spring stress male part 3 respectively, and the spring stress makes the spring stress female part 4 and the spring stress male part 3 separate or close, thereby controlling the relative rotation position of the rotating mechanism cover plate 2 on the wound surface fixing base 7 by the spring stress;

[0054] The wound surface fixing base 7 is provided with a rotation limiting column 10, and the rotating mechanism cover plate 2 is provided with a rotation limiting rail 16 matched with the rotation limiting column 10; the rotation limiting column 10 has a protruding part that acts as a buckle, and the protruding part is buckled into the rotation limiting rail 16, thereby realizing the relative rotation fixation between the rotating mechanism cover plate 2 and the wound surface fixing base 7;

[0055] The transparent observation cover plate 1 is fixedly installed on the rotating mechanism cover plate 2 by bolts, and completely covers the circular opening in the middle of the rotating mechanism cover plate 2; the transparent observation cover plate 1 is also provided with a scale line.

[0056] Application Example 1

[0057] The application example is based on the application method of the fixing device of embodiment 1, mainly including the following steps:

[0058] (1) Cut and punch the skin tissue of the experimental animal, cut and separate the adherent skin to form a circular full-thickness wound;

[0059] (2) The wound fixation base of the skin circular wound expander is pasted on the skin around the full-thickness wound with adhesive, so that the full-thickness wound is located in the circular opening of the wound fixation base, and the surrounding skin is further fixed by the outer suture hole on the wound fixation base; the rotating mechanism cover drives the expansion unit blade to form a combined center hole above the full-thickness wound, the size of the combined center hole matches the size of the full-thickness wound, and then the expansion unit blade and the skin around the full-thickness wound are sutured and fixed by using suture through the suture hole;

[0060] (3) Install the spring piece on the spring mounting shaft, separate the spring stress mother piece and the spring stress sub-piece by using the spring stress, and then control the rotation of the rotating mechanism cover on the wound fixation base by the spring stress, so as to apply traction force to the skin around the full-thickness wound in multiple different directions away from the wound.

[0061] The above embodiments are the preferred embodiments of the present application, but the embodiments of the present application are not limited by the above embodiments, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application shall be equivalent replacement methods, which are all included in the protection scope of the present application.

Claims

1. A skin round wound surface expansion fixator for animal experiments based on an aperture structure, characterized in that The skin circular wound expansion fixator is mainly composed of a wound fixation base, expansion unit blades, a rotating mechanism cover plate and spring components, The wound fixation base is a circular ring structure with a circular opening in the middle, and a plurality of blade limiting sliding grooves are arranged around the circular opening. The expansion unit blades are provided with limiting protrusions corresponding to the blade limiting sliding grooves, and when the plurality of expansion unit blades are limited by the limiting protrusions and move in the blade limiting sliding grooves of the wound fixation base, the plurality of expansion unit blades can form a combined central hole of different sizes. The expansion unit blades are provided with suture holes near one end of the combined central hole, and are also provided with blade driving sliding grooves. The rotating mechanism cover plate is a circular ring structure with a circular opening in the middle, and a plurality of driving protrusions corresponding to the blade driving sliding grooves are arranged around the circular opening. When the rotating mechanism cover plate drives the expansion unit blades, the rotating mechanism cover plate rotates relative to the wound fixation base.

2. The animal experiment skin round wound surface expansion fixator according to claim 1, characterized in that: Spring stress mother components and spring stress subcomponents are arranged on the wound fixation base and the rotating mechanism cover plate respectively, and the spring components are installed at both ends of the spring stress mother components and the spring stress subcomponents respectively.

3. The animal experimental skin round wound surface expansion fixator according to claim 2, characterized in that: The spring stress causes the spring stress mother components and the spring stress subcomponents to separate or approach each other, and then the rotation of the rotating mechanism cover plate on the wound fixation base is controlled by the spring stress.

4. The animal experiment skin round wound surface expansion fixator according to claim 1, characterized in that: A rotating limiting column is arranged on the wound fixation base, and a rotating limiting rail matching the rotating limiting column is arranged on the rotating mechanism cover plate.

5. The animal experimental skin round wound surface expansion fixator according to claim 1, characterized in that: The rotating limiting column has a protruding part that acts as a buckle, and the protruding part is buckled into the rotating limiting rail to realize the relative rotation fixation between the rotating mechanism cover plate and the wound fixation base.

6. The animal experiment skin round wound surface expansion fixator according to claim 1, characterized in that: A plurality of external suture holes are also arranged on the wound fixation base.

7. The animal experiment skin round wound surface expansion fixator according to claim 1, characterized in that: The blade limiting sliding grooves and the blade driving sliding grooves are straight grooves.

8. The animal experimental skin round wound surface expansion fixator according to claim 7, characterized in that: The spring stress mother components and the spring stress subcomponents are cylindrical shaft components that can rotate relative to their fixed positions, and a spring mounting shaft is fixedly installed on the spring stress subcomponent. The skin circular wound expansion fixator also includes a transparent observation cover plate that is installed on the rotating mechanism cover plate and completely covers the circular opening in the middle of the rotating mechanism cover plate. A scale line is also arranged on the transparent observation cover plate.

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