A microbial detection device and method

Abstract

The present invention discloses a microbial detection device, which relates to the technical field of microbial detection. It includes a supporting side plate. At the lower end of the side wall of the supporting side plate, a base is fixedly arranged, and at the upper end of the side wall of the supporting side plate, a top plate is fixedly arranged. At the center of the interior of the base, a servo motor is fixedly installed. The output end of the servo motor penetrates through the top of the base and is fixedly connected to a filtrate tank. Four groups of filtrate mechanisms are equiangularly arranged at the top of the filtrate tank. A cleaning mechanism is arranged at the bottom of the top plate, and an ultraviolet disinfection lamp is installed at the bottom of the top plate. In the detection process of the present invention, the operation is simple, and the working efficiency is high. Moreover, during filtration, on the one hand, the phenomenon of filter membrane blockage is reduced, and the filtration effect is improved. On the other hand, when the number of microorganisms is large, the microorganisms can be evenly distributed on the filter membrane, avoiding the stacking of microorganisms together, which is not convenient for later observation and counting.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism detection, and specifically provides a microorganism detection device and method. Background Art

[0002] There are many kinds of microorganism detections. Among them, the microorganism limit detection method is a common detection method. The principle of microorganism limit detection is mainly based on the chemical reaction characteristics of microorganisms. By detecting the enzyme activity in microbial cells and the number of microbial cells, the content of microorganisms is deduced. Usually, the membrane filtration method is used for detection.

[0003] During detection, the filter head needs to be disassembled first and the drain port of the bottom filtrate tank is cleaned with a cleaning solution. Then the filter head is inserted into the drain port, and the filter head is cleaned again with the cleaning solution. After cleaning, the filter head is burned with a blowtorch to evaporate the cleaning solution on its surface and inside. After the filter head cools down, the filter disc is placed on the top of the filter head, the filter membrane is placed on the filter head, and a filter cup is covered outside the filter disc. At this time, the prepared test solution can be poured into the filter cup. The valve connecting the filtrate tank to the filter head is opened, and the test solution in the filter cup is filtered through the filter membrane and the filter disc and discharged into the filtrate tank. The microorganisms in the test sample are retained on the filter membrane. The filter membrane is taken out and transferred to the prepared solid medium, with the bacterial surface facing up, lying flat, and the lid is covered to form a closed culture box, which is placed in the corresponding constant temperature incubator for cultivation and counting.

[0004] In the prior art, when performing microorganism limit detection in the above manner, the operation is relatively troublesome, the work efficiency is low, and when filtering samples with a large amount of microorganisms, it is easy to cause the filter membrane to be blocked before the sample solution is completely filtered, resulting in the sample solution being retained above the filter membrane and unable to be completely filtered, affecting subsequent detection steps. Summary of the Invention

[0005] The technical solution of the present invention aims at the technical problem that the prior art solution is too single, and provides a solution significantly different from the prior art. Specifically, the purpose of the present invention is to provide a microorganism detection device and method to solve the problems of troublesome operation, low work efficiency during detection, and easy blockage of the filter membrane before the sample solution is completely filtered when filtering samples with a large amount of microorganisms as mentioned in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A microorganism detection device includes support side plates. A base is fixedly arranged at the lower end of the side wall of the support side plates, and a top plate is fixedly arranged at the upper end of the side wall of the support side plates. A servo motor is fixedly installed at the center of the inside of the base. The output end of the servo motor penetrates through the top of the base and is fixedly connected to a filtrate tank. Four groups of filtrate mechanisms are arranged at equal angles on the top of the filtrate tank. A cleaning mechanism is arranged at the bottom of the top plate, and an ultraviolet disinfection lamp is installed at the bottom of the top plate.

[0007] Preferably, a liquid discharge valve port is provided at the bottom of the filtrate tank.

[0008] Preferably, the filtrate mechanism includes a notch opened at the top of the filtrate tank. A filter funnel is provided at the center of the notch. First springs are fixedly connected between the notch and the filter funnel at equal angles. A support is fixedly provided on the inner wall of the lower end of the filter funnel. A push rod slidably matched with the support is provided at the center of the support. A first pressing block is fixedly provided on the outer wall of the lower end of the push rod. A second spring is fixedly connected between the first pressing block and the support. A framework is provided on the inner wall of the upper end of the push rod within the filter funnel. An auxiliary driving assembly is provided on the outer wall of the filtrate tank on one side of the push rod.

[0009] Preferably, the framework includes a plurality of circular rings arranged longitudinally at equal intervals, and the diameters of the circular rings gradually decrease upward. The circular rings are fixedly connected to the push rod through connecting pieces. Protrusions are provided on the tops of the circular rings, and the circular ring at the bottom fits against the inner wall of the filter funnel.

[0010] Preferably, the auxiliary driving assembly includes two groups of movable rods slidably matched with the filtrate tank. One group of movable rods is located on one side of the lower end of the filter funnel, and the other group of movable rods is located on one side of the lower end of the push rod. Second pressing blocks are fixedly provided on the outer walls of one ends of the movable rods. Third springs are fixedly connected between the second pressing blocks and the outer wall of the filtrate tank. The other ends of the other group of movable rods are fixedly connected with a moving block inside the filtrate tank. An arc-shaped plate is fixedly installed on the outer wall of the base outside the filtrate tank. First guiding blocks are arranged at equal intervals on the upper end of the inner side wall of the arc-shaped plate. A second guiding block is fixedly provided on the lower end of the inner side wall of the arc-shaped plate.

[0011] Preferably, sealing members are provided between the two groups of movable rods and the filtrate tank. A slope is provided on the outer wall of the moving block on one side of the lower end of the push rod. The first guiding block and one group of movable rods are on the same horizontal plane, and the outer wall of the first guiding block is of an arc-shaped structure. The outer wall of the second guiding block is of a slope structure. The second guiding block and the other group of movable rods are on the same horizontal plane.

[0012] Preferably, the cleaning mechanism includes an outer cylinder fixedly arranged at the center of the top of the filtrate tank. Four liquid infusion pipes are connected to the outer wall of the outer cylinder at equal angles. The end parts of the four liquid infusion pipes are respectively fixedly connected with connectors located at the tops of the four filter funnels. The bottom of the connector is evenly provided with liquid outlets. The top of the outer cylinder is of an open structure. A bottom center of the top plate is fixedly provided with an inner cylinder located inside the outer cylinder. An outer wall of the inner cylinder is fixedly provided with a sealing ring that fits the inner wall of the outer cylinder. The inner cylinder and the sealing ring are provided with through grooves at the same horizontal plane as the liquid infusion pipes. A box body is arranged on the top of the top plate, and a liquid injection port is arranged on the top of the box body. A liquid outlet groove communicating with the inner cylinder is opened at the bottom of the box body and inside the top plate.

[0013] A microorganism detection method includes the following steps:

[0014] S1. Prepare the test solution sample to be detected and the culture medium for storing the filter membrane.

[0015] S2. Start the servo motor and the ultraviolet disinfection lamp, and place the filter membrane and filter the test solution sample after each filtrate mechanism rotates to the rightmost position and stops. After rotating 90 degrees, take out the filtered filter membrane.

[0016] S3. Transfer the taken-out filter membrane to the prepared solid culture medium, with the bacterial surface facing up, flatly attached, cover the lid to form a closed culture box, and place it in the corresponding constant temperature incubator for culturing and counting.

[0017] S4. After the filtrate mechanism rotates one full circle, under the action of the cleaning mechanism and the ultraviolet disinfection lamp, it is disinfected and sterilized and the cleaning liquid is dried, and then cooled for the next use.

[0018] Compared with the prior art, the beneficial effects of the present invention are:

[0019] (1) In the present invention, at the beginning stage of one full rotation of the filtrate mechanism, the filtrate mechanism can first filter the test solution to separate microorganisms. When rotating to the first 90 degrees, the test solution can be completely filtered and the filter membrane can be lifted up for convenient taking out. When rotating to the second 90 degrees, the cleaning mechanism can automatically clean the filtrate mechanism. When rotating to the third 90 degrees, the filtrate mechanism can be disinfected and sterilized by the ultraviolet disinfection lamp and the residual cleaning liquid can be dried. When rotating to the fourth 90 degrees, the filtrate mechanism can be cooled for the next use. The filter membrane can be used for filtration and taken out once every 90 degrees of rotation without subsequent operations, so that the operation of the device during detection is relatively simple and the work efficiency is improved.

[0020] (2) In the present invention, a filtrate mechanism is provided. The filter membrane is placed inside the filter funnel and supported by the ring at the top of the framework. The filter membrane slopes slightly from the middle to the outside. At this time, the test solution to be detected is slowly poured from above the ring at the top of the framework. During the pouring process, the liquid will press down on the filter membrane. Under the limiting action of the top of the ejector rod, the filter membrane forms a depression at the ring at the top of the framework. When the test solution is filtered, microorganisms are intercepted at the central depression. The permeability of the filter membrane gradually decreases. After the liquid in the central depression is full, the liquid diffuses and flows towards the outside of the filter membrane. The liquid presses the filter membrane against the protrusion at the top of the framework, and depressions are formed between the rings. Similarly, microorganisms are successively intercepted in the depression areas. The solution filtered from microorganisms flows through the bottom of the filter funnel into the filtrate tank for collection. If the number of microorganisms in the test solution is small, the test solution can be completely filtered at the upper-layer depression, which is convenient for centralized observation. If the number of microorganisms in the test solution is large, the microorganisms spread from the middle of the filter membrane to the outside. Thus, during the filtering process, on the one hand, the phenomenon of filter membrane blockage is reduced, and the filtering effect is improved. On the other hand, when the number of microorganisms is large, the microorganisms can be evenly distributed on the filter membrane, avoiding the stacking of microorganisms together, which is not convenient for later observation;

[0021] (3) In the present invention, during the first 90-degree rotation of the filtrate mechanism, under the guiding action of the equally spaced first guiding blocks at the end of the upper movable rod, the movable rod slides and drives the second pressing block on the outer wall to press the third spring. The other end of the movable rod pushes the bottom of the filter funnel. The filter funnel shakes slightly under the action of the first spring on the outer wall. As the movable rod continuously passes through the intermittently arranged first guiding blocks, the filter funnel shakes intermittently and resets under the action of the first spring. During the shaking process of the filter funnel, the accumulated liquid in the depression area of the filter membrane can be shaken out. Since there is a certain distance between the front bottom of the moving block and the bottom of the ejector rod, it will not affect the shaking of the filter funnel, thus enabling the complete filtration of the test solution. Description of the Drawings

[0022] Figure 1 is the front view structural diagram of the device of the present invention;

[0023] Figure 2 is the front view sectional structural diagram of the device of the present invention;

[0024] Figure 3 of the present invention Figure 2 is the enlarged view at A in;

[0025] Figure 4 is the top view structural diagram of the bracket of the present invention;

[0026] Figure 5 is the front view sectional structural diagram of the framework of the present invention;

[0027] Figure 6 is the top view structural diagram of the framework of the present invention;

[0028] Figure 7 It is a schematic top - view sectional structure diagram of the filtrate tank of the present invention;

[0029] Figure 8 It is a schematic structure diagram during the filtration of the filter membrane of the present invention;

[0030] Figure 9 It is a schematic top - view sectional structure diagram of the outer cylinder and the inner cylinder of the present invention;

[0031] Figure 10 It is a schematic bottom - structure diagram of the top plate of the present invention.

[0032] In the figure: 1, support side plate; 2, base; 3, top plate; 4, servo motor; 5, filtrate tank; 6, filter hopper; 61, first spring; 62, bracket; 63, ejector rod; 64, first pressing block; 65, second spring; 66, skeleton; 67, movable rod; 68, second pressing block; 69, third spring; 610, moving block; 611, arc plate; 612, first guide block; 613, second guide block; 7, outer cylinder; 71, infusion tube; 72, connector; 73, inner cylinder; 74, through - slot; 75, box body; 8, ultraviolet disinfection lamp. Specific embodiments

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] Please refer to Figures 1-10 , the present invention provides a technical solution: a microbial detection device, including a support side plate 1, a base 2 is fixedly arranged at the lower end of the side wall of the support side plate 1, and a top plate 3 is fixedly arranged at the upper end of the side wall of the support side plate 1. A servo motor 4 is fixedly installed at the center of the interior of the base 2, the output end of the servo motor 4 penetrates through the top of the base 2 and is fixedly connected to a filtrate tank 5. Four groups of filtrate mechanisms are arranged at equal angles on the top of the filtrate tank 5. A cleaning mechanism is arranged at the bottom of the top plate 3, and an ultraviolet disinfection lamp 8 is installed at the bottom of the top plate 3.

[0035] A drain valve port is arranged at the bottom of the filtrate tank 5.

[0036] The filtrate mechanism includes a notch opened at the top of the filtrate tank 5. A filter funnel 6 is provided at the center of the notch. First springs 61 are fixedly connected at equal angles between the notch and the filter funnel 6. A bracket 62 is fixedly provided on the inner wall of the lower end of the filter funnel 6. A ejector rod 63 that is slidably matched with the bracket 62 is provided at the center of the bracket 62. A first pressing block 64 is fixedly provided on the outer wall of the lower end of the ejector rod 63. A second spring 65 is fixedly connected between the first pressing block 64 and the bracket 62. A framework 66 is provided on the inner wall of the upper end of the ejector rod 63 within the filter funnel 6. An auxiliary driving assembly is provided on the outer wall of the filtrate tank 5 on one side of the ejector rod 63.

[0037] The framework 66 includes a number of circular rings arranged at equal intervals longitudinally, and the diameters of the circular rings gradually decrease upward. And the circular rings are fixedly connected to the ejector rod 63 through connectors. Protrusions are provided at the tops of the circular rings, and the circular ring at the bottom fits against the inner wall of the filter funnel 6.

[0038] The auxiliary driving assembly includes two groups of movable rods 67 that are slidably matched with the filtrate tank 5. One group of movable rods 67 is located on one side of the lower end of the filter funnel 6, and the other group of movable rods 67 is located on one side of the lower end of the ejector rod 63. Second pressing blocks 68 are fixedly provided on the outer walls of one ends of the movable rods 67. Third springs 69 are fixedly connected between the second pressing blocks 68 and the outer wall of the filtrate tank 5. The other ends of the other group of movable rods 67 are fixedly connected with a moving block 610 inside the filtrate tank 5. An arc-shaped plate 611 is fixedly installed on the outer wall of the base 2 outside the filtrate tank 5. First guide blocks 612 are arranged at equal intervals on the upper end of the inner side wall of the arc-shaped plate 611. A second guide block 613 is fixedly provided on the lower end of the inner side wall of the arc-shaped plate 611.

[0039] Sealing members are provided between the two groups of movable rods 67 and the filtrate tank 5. A slope is provided on the outer wall of the moving block 610 on one side of the lower end of the ejector rod 63. The first guide block 612 and one group of movable rods 67 are on the same horizontal plane, and the outer wall of the first guide block 612 is an arc-shaped structure. The outer wall of the second guide block 613 is a slope structure, and the second guide block 613 and the other group of movable rods 67 are on the same horizontal plane.

[0040] The cleaning mechanism includes an outer cylinder 7 fixedly provided at the center of the top of the filtrate tank 5. Four groups of infusion pipes 71 are connected to the outer wall of the outer cylinder 7 at equal angles. The ends of the four groups of infusion pipes 71 are respectively fixedly connected with connectors 72 located on the tops of the four groups of filter funnels 6. Liquid outlet holes are evenly provided at the bottoms of the connectors 72. The top of the outer cylinder 7 is an open structure. An inner cylinder 73 located inside the outer cylinder 7 is fixedly provided at the center of the bottom of the top plate 3. A sealing ring that fits against the inner wall of the outer cylinder 7 is fixedly provided on the outer wall of the inner cylinder 73. Through grooves 74 on the same horizontal plane as the infusion pipes 71 are provided on the inner cylinder 73 and the sealing ring. A box body 75 is provided on the top of the top plate 3, and a liquid injection port is provided on the top of the box body 75. And an liquid outlet groove communicating with the inner cylinder 73 is provided at the bottom of the box body 75 and inside the top plate 3.

[0041] A microbial detection method, comprising the following steps:

[0042] S1, Prepare the test solution sample to be detected and the culture medium for storing the filter membrane;

[0043] S2, Start the servo motor 4 and the ultraviolet disinfection lamp 8, and place the filter membrane and filter the test solution sample after each set of filtrate mechanisms rotates to the rightmost position and stops. After rotating 90 degrees, take out the filtered filter membrane;

[0044] S3, Transfer the taken-out filter membrane to the prepared solid culture medium, with the bacterial surface facing up, flatly attached, cover the lid to form a closed culture box, and place it in the corresponding constant temperature incubator for culturing and counting;

[0045] S4, After the filtrate mechanism rotates one full circle, it is disinfected and sterilized and the cleaning solution is dried under the action of the cleaning mechanism and the ultraviolet disinfection lamp 8, and is cooled for the next use.

[0046] Working principle: When using this microbial detection device and method, first, start the servo motor 4. The servo motor 4 drives the filtrate tank 5 to rotate slowly regularly, and each rotation angle is 90 degrees. When the filtrate tank 5 rotates, it will drive the filtrate mechanism on the top to rotate. During the process of each set of filtrate mechanisms rotating one full circle, when the filter funnel 6 rotates to the rightmost position, place the filter membrane in the filter funnel 6, and the filter membrane is supported by the ring at the top of the skeleton 66. Since the filter membrane is made of a soft material, the filter membrane tilts slightly from the middle to the outside. At this time, slowly pour the test solution to be detected from above the ring at the top of the skeleton 66. During the pouring process, the liquid will press down on the filter membrane. Under the top limit of the top rod 63, the filter membrane forms a depression at the ring at the top of the skeleton 66. When the test solution is filtered, microorganisms are intercepted in the central depression. The permeability of the filter membrane gradually decreases. After the central depression is filled with liquid, since the rings in the skeleton 66 gradually decrease from top to bottom, the liquid diffuses and flows to the outside of the filter membrane. The liquid presses the filter membrane against the protrusion at the top of the skeleton 66, and depressions are formed between the rings. Similarly, microorganisms are intercepted in the depression areas in turn. The solution filtered out of the microorganisms flows through the bottom of the filter funnel 6 into the filtrate tank 5 for collection. If the number of microorganisms in the test solution is small, the test solution can be completely filtered in the upper depression, which is convenient for centralized observation. If the number of microorganisms in the test solution is large, the microorganisms will spread from the middle of the filter membrane to the outside. Therefore, during the filtration process, on the one hand, the phenomenon of filter membrane blockage is reduced, and the filtration effect is improved. On the other hand, when the number of microorganisms is large, the microorganisms can be evenly distributed on the filter membrane, avoiding the stacking of microorganisms together, which is not convenient for later observation;

[0047] When the filter membrane is pressed to be inclined, the edge of the filter membrane just fits on the top protrusion of the bottommost ring of the skeleton 66, and the microorganisms have strong adsorption after being intercepted on the surface of the filter membrane and will not move with the liquid flow;

[0048] After pouring out a certain amount of the test solution, the filtrate tank 5 drives this group of filter funnels 6 to continue rotating. During the process of rotating the next ninety degrees, under the guiding action of the first guiding blocks 612 arranged at equal intervals, the end of the upper movable rod 67 slides, and the second pressing block 68 on the outer wall is driven to press the third spring 69. The other end of the movable rod 67 pushes the bottom of the filter funnel 6. The filter funnel 6 shakes slightly under the action of the first spring 61 on the outer wall. As the movable rod 67 continuously passes the spaced-apart first guiding blocks 612, the filter funnel 6 shakes intermittently and resets under the action of the first spring 61. The filter funnel 6 can shake out the accumulated liquid in the sunken area of the filter membrane during the shaking process. Since there is a certain distance between the bottom of the front end of the moving block 610 and the bottom of the ejector rod 63, it will not affect the shaking of the filter funnel 6, thus enabling the complete filtration of the test solution;

[0049] When about to rotate to ninety degrees, under the guiding action of the second guiding block 613, the lower movable rod 67 also slides on the outer wall of the filtrate tank 5 in the same way, and this movable rod 67 pushes the moving block 610 to move. Under the guiding action of the inclined surface of the moving block 610, the ejector rod 63 slides upward in the bracket 62, and the ejector rod 63 drives the first pressing block 64 on the outer wall to squeeze the second spring 65. When the end of the movable rod 67 moves to the highest point of the inclined surface of the second guiding block 613, the ejector rod 63 pushes the filter membrane at the top to the upper end of the filter funnel 6 through the framework 66, which is convenient for the staff to take it out. After taking it out, the filter membrane is transferred to the prepared solid medium, with the bacterial surface facing up, lying flat, covering the lid to form a closed culture box, and placed in the corresponding constant temperature incubator for culturing and counting;

[0050] During the process of rotating to the next ninety degrees, since during the previous rotation, the through groove 74 did not coincide with the infusion tube 71 corresponding to this filter funnel 6, the cleaning liquid inside the inner cylinder 73 did not flow out through the infusion tube 71 and the liquid outlet at the bottom of the connector 72. However, within the next ninety-degree range, the through groove 74 coincides with the infusion tube 71, and the cleaning liquid flows out from the liquid outlet at the bottom of the connector 72 and pours on the filter funnel 6 to clean the filter funnel 6 and its interior, and the cleaning liquid flows into the filtrate tank 5 for collection until the through groove 74 and the infusion tube 71 are misaligned again, and the cleaning of the filter funnel 6 stops;

[0051] When rotating to the third ninety-degree angle, the cleaned filter hopper 6 passes under the ultraviolet disinfection lamp 8. Within this range, the filter hopper 6 is disinfected and sterilized, and the ultraviolet disinfection lamp 8 has the effect of heating and drying, which can dry the cleaning liquid remaining in the filter hopper 6. When rotating to the fourth ninety-degree angle, the filter hopper 6 at a certain temperature is cooled until it rotates back to the starting point of the first ninety-degree angle to perform filtration and subsequent processing again. Then, when the four groups of filtrate mechanisms rotate simultaneously, filtration can be carried out once every ninety-degree rotation. After the detection is completed, turn off the servo motor 4 and the ultraviolet disinfection lamp 8. The waste liquid collected at the bottom of the filtrate tank 5 can be discharged, and there is always cleaning liquid in the inner cylinder 73. When it is almost used up, cleaning liquid can be added through the liquid injection port at the top of the box body 75, and the cleaning liquid will flow into the inner cylinder 73 through the liquid outlet groove.

[0052] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A microorganism detection device, comprising a supporting side plate (1), characterized in that: A base (2) is fixedly provided at the lower end of the side wall of the supporting side plate (1), and a top plate (3) is fixedly provided at the upper end of the side wall of the supporting side plate (1); a servo motor (4) is fixedly installed at the inner center of the base (2); an output end of the servo motor (4) passes through the top of the base (2) and is fixedly connected to a filtrate box (5); four groups of filtrate mechanisms are arranged at equal angles on the top of the filtrate box (5); a cleaning mechanism is arranged at the bottom of the top plate (3), and an ultraviolet disinfection lamp (8) is installed at the bottom of the top plate (3); The filtrate mechanism comprises a notch opened at the top of the filtrate box (5), a filter bucket (6) is arranged at the center of the notch, a first spring (61) is fixedly connected at an equal angle between the notch and the filter bucket (6), a bracket (62) is fixedly arranged on the inner wall of the lower end of the filter bucket (6), a push rod (63) slidably matched with the bracket (62) is arranged at the center of the bracket (62), a frame (66) is arranged at the upper end of the push rod (63) on the inner wall of the filter bucket (6), and an auxiliary drive component is arranged on the outer wall of the filtrate box (5) on one side of the push rod (63); The skeleton (66) includes a plurality of circular rings arranged at equal intervals in the longitudinal direction, and the diameters of the circular rings decrease in sequence upwards, and the circular rings are fixedly connected to the top rod (63) by connecting pieces, and the tops of the circular rings are provided with protrusions, and the circular rings at the bottom are in contact with the inner wall of the filter bucket (6); The auxiliary drive assembly comprises a movable rod (67) that is slidably matched with the filtrate box (5), and a group of the movable rods (67) are located on one side of the lower end of the filter bucket (6); the outer wall of the base (2) is located outside the filtrate box (5) and is fixedly mounted with an arc plate (611), and the upper end of the inner wall of the arc plate (611) is evenly spaced with first guide blocks (612); as the movable rod (67) continuously passes through the first guide blocks (612) that are spaced apart, the filter bucket (6) intermittently shakes and is reset under the action of the first spring (61).

2. A microorganism detection device according to claim 1, characterized in that: The bottom of the filtrate box (5) is provided with a drain valve port.

3. A microorganism detection device according to claim 1, characterized in that: A first pressing block (64) is fixedly provided on the outer wall of the lower end of the push rod (63), and a second spring (65) is fixedly connected between the first pressing block (64) and the bracket (62).

4. A microorganism detection device according to claim 3, characterized in that: The auxiliary drive assembly includes two groups of movable rods (67) that are slidably matched with the filtrate box (5), one group of the movable rods (67) is located on one side of the lower end of the filter bucket (6), and the other group of the movable rods (67) is located on one side of the lower end of the top rod (63). A second pressure block (68) is fixedly provided on the outer wall of one end of the movable rod (67), and a third spring (69) is fixedly connected between the second pressure block (68) and the outer wall of the filtrate box (5). The other end of the other group of movable rods (67) is located inside the filtrate box (5) and is fixedly connected to a moving block (610), and a second guide block (613) is fixedly provided on the lower end of the inner wall of the arc plate (611).

5. A microorganism detection device according to claim 4, characterized in that: A seal is provided between the two groups of movable rods (67) and the filtrate box (5); the outer wall of the movable block (610) is provided with an inclined surface on one side of the lower end of the top rod (63); the first guide block (612) and a group of movable rods (67) are located on the same horizontal plane, and the outer wall of the first guide block (612) is an arc-shaped structure; the outer wall of the second guide block (613) is an inclined surface structure; the second guide block (613) and another group of movable rods (67) are located on the same horizontal plane.

6. A microorganism detection device according to claim 1, characterized in that: The cleaning mechanism comprises an outer cylinder (7) fixedly arranged at the top center of the filtrate box (5), the outer wall of the outer cylinder (7) being connected with four groups of infusion tubes (71) at equal angles, the ends of the four groups of infusion tubes (71) being respectively fixedly connected with connectors (72) located at the tops of the four groups of filter buckets (6), the bottoms of the connectors (72) being evenly provided with liquid outlets, the top of the outer cylinder (7) being an open structure, the bottom center of the top plate (3) being fixedly provided with a liquid outlet located at the outer cylinder (7), and the bottom center of the top plate (3) being fixed with a liquid outlet located at the outer cylinder (7). An inner tube (73) is disposed inside the tube (7), a sealing ring is fixedly disposed on the outer wall of the inner tube (7) and is in contact with the inner wall of the outer tube (7), a through groove (74) is provided on the inner tube (73) and the sealing ring and is located at the same level as the infusion tube (71), a box body (75) is disposed on the top of the top plate (3), a liquid injection port is disposed on the top of the box body (75), and a liquid outlet groove communicating with the inner tube (73) is disposed on the bottom of the box body (75) and the inside of the top plate (3).

7. A detection method for a microorganism detection device according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1, prepare the test solution sample to be tested and the culture medium for storing the filter membrane; S2, starting the servo motor (4) and the ultraviolet disinfection lamp (8), and placing the filter membrane and filtering the test liquid sample after each group of the filtrate mechanism rotates to the rightmost side and stops, and then taking out the filtered filter membrane after rotating 90 degrees; S3, transfer the removed filter membrane to the prepared fixed culture medium, with the bacterial surface facing upwards, flat, cover with a lid to form a closed culture box, place in a corresponding constant temperature incubator for culture and counting; S4, after the filtrate mechanism has completed one rotation, the cleaning mechanism and the ultraviolet disinfection lamp (8) are used to disinfect and sterilize the cleaning liquid, dry the cleaning liquid, and cool it for the next use.

Images