An anti-wrinkle repairing composition and its preparation method and application

Abstract

The present invention discloses an anti-wrinkle repairing composition and a preparation method and application thereof, belonging to the technical field of cosmetics. The composition comprises: rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, Lepidium SATIVUM bud extract, succinylated atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside and polyol; the present invention has obvious synergistic effect through scientific combination and compounding of multiple plant extracts and collagen, can quickly repair the skin barrier, effectively increase skin elasticity, reduce wrinkles, and achieve the effect of anti-wrinkle repairing.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-wrinkle repairing composition and a preparation method and application thereof. Background Art

[0002] As we age, the collagen and elastin in our skin gradually decrease, leading to sagging skin and deeper wrinkles. Consumers have a strong desire to maintain a youthful appearance, and the demand for anti-wrinkle skin care products is also growing.

[0003] With the continuous development of science and technology, the information gap between brands and consumers is also being bridged. Consumers' awareness of cosmetic ingredients is increasing day by day. Consumers pay more attention to "product efficacy", "ingredients" and "technology", which have become the main factors affecting the purchase of repair and anti-wrinkle skin care products. There are many anti-wrinkle ingredients, such as vitamin A acid, vitamin C, vitamin E, collagen, ursolic acid, bosine, bioactive peptides, etc. Single anti-aging and anti-wrinkle ingredients are limited, and the combination of multiple ingredients is the main direction of research at this stage.

[0004] There are many anti-aging and anti-wrinkle products on the market, but they generally have problems such as poor permeability, poor absorption, irritation, and the anti-wrinkle effect needs to be improved. Therefore, it is of great significance to provide a safe, easy to absorb, strong anti-wrinkle effect, and skin repair cosmetics. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-wrinkle repairing composition and its preparation method and application, which is a combination of collagen and plant extracts that are easily absorbed by the skin, is mild, safe, and has good permeability, and can better promote skin barrier repair, enhance skin elasticity, and reduce wrinkles.

[0006] To this end, the first technical solution provided by the present invention is:

[0007] An anti-wrinkle repairing composition comprises the following components in parts by weight: 1-20 parts of rice (ORYZA SATIVA) germ extract, 1-20 parts of olive (OLEA EUROPAEA) bud extract, 1-20 parts of LEPIDIUM SATIVUM bud extract, 0.1-10 parts of succinyl atelocollagen, 0.01-7 parts of soluble collagen cross-linked polymer, 0.01-7 parts of hydrolyzed collagen zinc, 0.01-9 parts of stevioside and 10-120 parts of polyol.

[0008] Preferably, the anti-wrinkle repairing composition comprises the following components in parts by weight: 3-15 parts of rice (ORYZA SATIVA) germ extract, 3-15 parts of olive (OLEA EUROPAEA) bud extract, 3-15 parts of LEPIDIUM SATIVUM) bud extract, 1-8 parts of succinyl atelocollagen, 0.1-5 parts of soluble collagen cross-linked polymer, 0.1-5 parts of hydrolyzed collagen zinc, 0.1-6 parts of stevioside and 20-80 parts of polyol.

[0009] Preferably, the anti-wrinkle repairing composition comprises the following components in parts by weight: 5-10 parts of rice (ORYZA SATIVA) germ extract, 5-10 parts of olive (OLEA EUROPAEA) bud extract, 5-10 parts of Lepidium SATIVUM bud extract, 1-3 parts of succinyl atelocollagen, 0.5-3 parts of soluble collagen cross-linked polymer, 0.5-3 parts of hydrolyzed collagen zinc, 0.5-3 parts of stevioside and 25-50 parts of polyol.

[0010] Preferably, the molecular weight of the succinyl atelocollagen ranges from 1KDa to 3000KD.

[0011] More preferably, the molecular weight of the succinyl atelocollagen ranges from 5KDa to 2000KD.

[0012] More preferably, the molecular weight of the succinyl atelocollagen ranges from 50KDa to 500KD.

[0013] More preferably, the molecular weight of the succinyl atelocollagen ranges from 100KDa to 300KD.

[0014] Preferably, the polyol is one or more of ethylene glycol, propylene glycol, butanediol, and 1,2-pentanediol.

[0015] The second technical solution provided by the present invention is:

[0016] A method for preparing an anti-wrinkle repairing composition comprises the following steps:

[0017] (1) Add the polyol into a stirring pot, heat to 30-42° C., and keep warm for 10-15 minutes;

[0018] (2) The succinylated atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside, rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, and Lepidium Sativum (LEPIDIUMSATIVUM) bud extract are sequentially added to the polyol and stirred evenly to obtain the composition.

[0019] The third technical solution provided by the present invention is:

[0020] The invention discloses an application of an anti-wrinkle repairing composition in cosmetics. The cosmetics include lotion, toner, softener, cream, emulsion, facial mask and essence.

[0021] In the present invention:

[0022] Succinyl atelocollagen has a small molecular weight and can penetrate the surface of the skin to fill the gaps in collagen, which helps to improve the skin's reconstruction and self-repair capabilities and reduce wrinkles and other blemishes caused by aging or damage to the skin. Succinyl atelocollagen has a small molecule and strong activity, which can better penetrate the surface of the skin, thereby ensuring that moisture enters the skin and forms a water-retaining layer, making the skin smoother, softer and more elastic.

[0023] Soluble collagen cross-linked polymer (trade name: human-like cross-linked collagen) has higher stability and mechanical properties than soluble collagen, and can better maintain its structure and function. It can replenish the collagen needed by the skin, improve the elasticity and moisture of the skin, effectively delay skin aging, make the skin very delicate and smooth, and has a strong ability to replenish moisture.

[0024] Hydrolyzed Collagen Zinc combines hydrolyzed collagen and zinc to provide dual benefits to the skin. Hydrolyzed collagen provides the skin with the collagen it needs to enhance its elasticity and moisturization, while zinc aids in skin cell renewal and repair while reducing inflammation. In addition, the combination may provide additional protection to the skin, helping to protect against environmental damage and speeding up wound healing.

[0025] Lepidium sativum sprout extract can effectively activate the activity of proteasomes and promote the production of skin collagen and elastin, thereby improving spots and wrinkles caused by aging. Rice (ORYZA SATIVA) germ extract is taken from the germ of rice essence and has good antioxidant and skin-revitalizing effects. Olive (OLEA EUROPAEA) sprout extract has antioxidant, free radical scavenging, skin-revitalizing and moisturizing effects. Stevioside has anti-inflammatory, anti-allergic, antioxidant and free radical scavenging effects.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The anti-wrinkle repair composition provided by the present invention is scientifically combined with three plant extracts of rice (ORYZA SATIVA) germ extract, olive (OLEAEUROPAEA) bud extract, and Lepidium SATIVUM bud extract, succinylated atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, and stevioside. The components have a mutual promotion effect, synergistically enhancing the skin barrier repair, improving skin elasticity and improving wrinkle effects. Experiments have confirmed that the composition of the present invention can promote the expression of Z0-1 protein in HaCaT cells, effectively increase the cell activity of keratinocytes, and promote the proliferation of keratinocytes, thereby helping to improve the skin barrier function, promote the re-epithelialization of the skin or mucous membranes, and accelerate the healing of the skin or mucous membranes, and can be used to repair the skin or mucous membranes.

[0028] The succinylated atelocollagen, soluble collagen cross-linked polymer, and hydrolyzed collagen zinc in the composition of the present invention can quickly penetrate the skin surface and replenish the collagen required by the skin, while the extract of Lepidium sativum sprouts can effectively activate the activity of proteasomes and promote the production of skin collagen and elastin, coordinately improve the elasticity of the skin, and promote cell renewal and repair; the rice germ extract and olive sprout extract can remove free radicals and supplement nutrients for the skin; stevioside can resist inflammation, allergy, and free radicals, and improve damaged skin. Through the mutual promotion of each component, the skin condition is coordinated to improve, the skin elasticity is enhanced, wrinkles are lightened, and an unexpected anti-wrinkle repair effect is exerted. DETAILED DESCRIPTION

[0029] The specific implementation methods of the technical solution of this specification are further described below through specific examples. These examples are for the purpose of describing the technical solution in detail, rather than for limiting the technical solution. Based on the examples in this specification, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this specification.

[0030] Unless otherwise specified, the experimental reagents and materials used in the present invention can be obtained commercially.

[0031] The molecular weight of succinyl atelocollagen in the following examples is 1500 KD.

[0032] Example 1

[0033] An anti-wrinkle repairing composition comprises the following components: 20 g of rice (ORYZA SATIVA) germ extract, 20 g of olive (OLEA EUROPAEA) bud extract, 20 g of LEPIDIUM SATIVUM bud extract, 10 g of succinylated atelocollagen, 5 g of soluble collagen cross-linked polymer, 5 g of hydrolyzed collagen zinc, 5 g of stevioside, and 80 g of 1,2-pentanediol.

[0034] Example 2

[0035] An anti-wrinkle repairing composition comprises the following components: 15 g of rice (ORYZA SATIVA) germ extract, 15 g of olive (OLEA EUROPAEA) bud extract, 10 g of LEPIDIUM SATIVUM bud extract, 5 g of succinylated atelocollagen, 3 g of soluble collagen cross-linked polymer, 5 g of hydrolyzed collagen zinc, 5 g of stevioside, and 60 g of 1,2-pentanediol.

[0036] Example 3

[0037] An anti-wrinkle repairing composition comprises the following components: 5g of rice (ORYZA SATIVA) germ extract, 5g of olive (OLEA EUROPAEA) bud extract, 10g of LEPIDIUM SATIVUM bud extract, 3g of succinylated atelocollagen, 3g of soluble collagen cross-linked polymer, 3g of hydrolyzed collagen zinc, 3g of stevioside, and 35g of 1,2-pentanediol.

[0038] Preparation Example

[0039] A method for preparing an anti-wrinkle repairing composition comprises the following steps:

[0040] (1) Add the polyol into a stirring pot, heat to 40° C., and keep warm for 15 minutes;

[0041] (2) The succinylated atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside, rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, and Lepidium SATIVUM bud extract are sequentially added to the polyol solution and stirred evenly to obtain the composition.

[0042] Comparative Example 1

[0043] Compared with Example 1, the difference is that rice (ORYZA SATIVA) germ extract is not included, and other components are the same. The preparation method is the same as that of Example 1.

[0044] Comparative Example 2

[0045] Compared with Example 1, the difference is that the olive (OLEA EUROPAEA) bud extract is not included, and the other components are the same. The preparation method is the same as that of Example 1.

[0046] Comparative Example 3

[0047] Compared with Example 1, the difference is that the extract of Lepidium sativum sprout is not included, and the other components are the same. The preparation method is the same as that of Example 1.

[0048] Comparative Example 4

[0049] Compared with Example 1, the difference is that succinyl atelocollagen is not included, and other components are the same. The preparation method is the same as that of Example 1.

[0050] Comparative Example 5

[0051] Compared with Example 1, the difference is that the soluble collagen cross-linked polymer is not included, and the other components are the same. The preparation method is the same as that of Example 1.

[0052] Comparative Example 6

[0053] Compared with Example 1, the difference is that hydrolyzed collagen zinc is not included, and other components are the same. The preparation method is the same as that of Example 1.

[0054] Comparative Example 7

[0055] Compared with Example 1, the difference is that stevioside is not included, and other components are the same. The preparation method is the same as that of Example 1.

[0056] Comparative Example 8

[0057] Compared with Example 1, the difference is that rice (ORYZA SATIVA) germ extract and olive (OLEAEUROPAEA) bud extract are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0058] Comparative Example 9

[0059] Compared with Example 1, the difference is that rice (ORYZA SATIVA) germ extract and Lepidium SATIVUM (LEPIDIUM SATIVUM) sprout extract are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0060] Comparative Example 10

[0061] Compared with Example 1, the difference is that Lepidium sativum bud extract and olive (Olea europaea) bud extract are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0062] Comparative Example 11

[0063] Compared with Example 1, the difference is that succinyl atelocollagen and soluble collagen cross-linked polymer are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0064] Comparative Example 12

[0065] Compared with Example 1, the difference is that succinyl atelocollagen and hydrolyzed collagen zinc are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0066] Comparative Example 13

[0067] Compared with Example 1, the difference is that hydrolyzed collagen zinc and soluble collagen cross-linked polymer are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0068] Comparative Example 14

[0069] Compared with Example 1, the difference is that succinyl atelocollagen and rice (ORYZA SATIVA) germ extract are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0070] Comparative Example 15

[0071] Compared with Example 1, the difference is that succinyl atelocollagen, rice (ORYZA SATIVA) germ extract, soluble collagen cross-linked polymer and stevioside are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0072] Comparative Example 16

[0073] Compared with Example 1, the difference is that succinyl atelocollagen, rice (ORYZA SATIVA) germ extract, soluble collagen cross-linked polymer, and hydrolyzed collagen zinc are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0074] Comparative Example 17

[0075] Compared with Example 1, the difference is that succinyl atelocollagen, rice (ORYZA SATIVA) germ extract, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, and stevioside are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0076] Comparative Example 18

[0077] Compared with Example 1, the difference is that rice (ORYZA SATIVA) germ extract, Lepidium SATIVUM bud extract, olive (OLEA EUROPAEA) bud extract, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, and stevioside are not included, and other components are the same. The preparation method is the same as that of Example 1.

[0078] Sample preparation example

[0079] An anti-wrinkle repair skin care product, comprising: 0.05% disodium EDTA, 0.2% carbomer, 0.1% sodium hyaluronate, 5% glycerol, 1.2% cetearyl olivate, 0.8% sorbitan olivate, 1.5% avocado butter, 4% caprylic / caprylic triglyceride, 3% isononyl isononanoate, 3% 1,3-pentanediol, 0.5% p-hydroxyacetophenone, 10% anti-wrinkle repair composition, and the balance being water. The preparation method is the same as that of Example 1.

[0080] To demonstrate the advantages of the present invention, the following efficacy tests were performed:

[0081] Experimental Example 1 Detection of Z0-1 protein expression in HaCaT cells

[0082] Experimental method: The compositions provided in Examples 1-3 and Comparative Examples 1-18 were used for the experiment. HaCaT cells were inoculated in a 12-well plate containing 10% fetal bovine serum and 1% double-antibody DMEM high-glucose medium at a density of 1×10 5 / well, after culturing for 24 hours, except for the blank group, the other wells were the anti-wrinkle repair composition intervention group, and the culture medium containing the composition was added, and the amount of the composition added was 50μ g / ml. The experiment ended after 24 hours, and the RT-PCR method was used to detect the gene expression of epidermal tight junction-related protein ZO-1. Each index was measured 3 times, and the average Ct value was taken. With β-actin as the internal reference gene, the relative quantitative method was used, and the relative expression content of the target gene = 2-ΔΔCt. The test results are shown in Table 1.

[0083] Epidermal cell tight junctions are considered the "master controller" of epidermal barrier function. Tight junction proteins (TJs) are important structures that maintain the mechanical barrier function of the epidermis. ZO proteins directly bind to actin, thereby forming a stable connection between the cytoskeleton and the TJ complex. ZO-1 is an important component protein of the ZO protein family.

[0084] It can be seen from the test results in Table 1 that, compared with the blank group, the anti-wrinkle repair composition provided by Examples 1-3 significantly increased the expression level of Z0-1 protein (P < 0.01), and Comparative Examples 1-18 promoted the expression of Z0-1 protein to varying degrees, and only Comparative Examples 2 and 4 had significant differences. Compared with Comparative Examples 1-18, Examples 1-3 had a more obvious promoting effect on the expression level of Z0-1 protein. Compared with Example 1, Comparative Examples 1-7 added less succinyl atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside, rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, and Lepidium SATIVUM bud extract, respectively, and the expression level of ZO-1 protein decreased significantly, indicating that the seven components acted synergistically to promote the expression of ZO-1 protein, and the lack of any of the components would reduce the expression level of ZO-1 protein; Compared with Example 1, Comparative Examples 8-18 lacked at least two or more components, and the expression level of ZO-1 protein decreased more significantly. It shows that the components in the composition provided by the present invention act together and synergistically to promote the expression of ZO protein, maintain the tight junction structure, and thus promote skin and barrier repair.

[0085] Table 1 Effect of the composition on the expression of ZO-1 protein in HaCaT cells

[0086]

[0087]

[0088] Compared with the blank group, *P<0.05 indicates a significant difference, and **P<0.01 indicates an extremely significant difference.

[0089] Test Example 2 Anti-wrinkle test

[0090] 1. Skin elasticity test

[0091] Test method: Select several volunteers aged 40-55 years old and randomly divide them into 21 groups, 10 people in each group, 105 men and women, and use skin care products 1-21 containing the compositions of the embodiments and comparative examples respectively. Take the skin on the inner forearm of the volunteer as the test site, apply 2.0±0.05g of anti-wrinkle repair skin care product once a day for 45 consecutive days. According to the test standard T / ZHCA 005-2019 "Test method for the effect of cosmetics on skin elasticity", the skin elasticity value R is measured before application and after 45 days of use. The larger the R value, the better the skin elasticity. The calculation formula of the R value is: R=U 1 / U 2 , where U 1 To eliminate the rebound displacement of the skin after negative pressure (mm), U2 is the maximum tensile displacement of the skin when negative pressure is applied (mm). The test results are shown in Table 2.

[0092] 2. Anti-wrinkle and lightening test

[0093] Female volunteers aged 40-55 were selected as test subjects and randomly divided into 21 groups, with 10 people in each group. Skin care products 1-21 containing the compositions of the embodiments and comparative examples were used respectively. They were used twice a day, once in the morning and once in the evening, applied on the face, and massaged for full absorption for 45 days. Wrinkles were tested using a VISIA skin detector, and the wrinkle depth (D 0 ) and wrinkle depth after 45 days of use (D 4 5), and then calculate the improvement rate of wrinkles. The calculation formula of the improvement rate of wrinkles is: Improvement rate (%) = (D 0 -D 45 ) / DO×100%. The test results are shown in Table 2.

[0094] Table 2 Anti-wrinkle and lightening test results

[0095]

[0096]

[0097] It can be seen from the test results in Table 2 that the anti-wrinkle repair skin care products of the present invention have good effects of increasing skin elasticity, tightening skin, and reducing wrinkles. After 45 days of continuous use, the skin elasticity value increase rate can reach 45.76%, and the wrinkle improvement rate can reach 38.5%. Compared with Example 1, Comparative Examples 1-7 add one less component, the elasticity increase rate decreases by about 6.2-14.2%, and the wrinkle improvement rate decreases by 9.0-10.6%; Comparative Examples 8-18 add two or more less components compared with Example 1, the elasticity value increase rate decreases by 32.86-42.36%, and the wrinkle improvement rate decreases by 20.4-36.5%. It can be seen that the succinylated atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside, rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, and Lepidium Sativum (LEPIDIUMSATIVUM) bud extract components of the present invention promote each other in improving skin elasticity and anti-wrinkle effects, have an unexpected synergistic effect, and can significantly increase the product's effect of imparting skin elasticity and reducing skin wrinkles.

[0098] Therefore, the anti-wrinkle and skin repairing composition provided by the present invention has good anti-wrinkle and skin repairing effects through reasonable combination, mutual promotion between components, and synergistic effect to repair skin barrier, enhance skin elasticity and reduce skin fine lines.

[0099] Although the embodiments of the present description have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present description, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An anti-wrinkle repairing composition, characterized in that: The invention comprises the following components in parts by weight: 20 parts of rice (ORYZA SATIVA) germ extract, 20 parts of olive (OLEA EUROPAEA) bud extract, 20 parts of LEPIDIUM SATIVUM bud extract, 10 parts of succinyl atelocollagen, 5 parts of soluble collagen cross-linked polymer, 5 parts of hydrolyzed collagen zinc, 5 parts of stevioside and 80 parts of polyol; The molecular weight of the succinyl atelocollagen is 1500KD; and the polyol is 1,2-pentanediol.

2. An anti-wrinkle repairing composition, characterized in that: The invention comprises the following components in parts by weight: 15 parts of rice (ORYZA SATIVA) germ extract, 15 parts of olive (OLEA EUROPAEA) bud extract, 10 parts of LEPIDIUM SATIVUM bud extract, 5 parts of succinyl atelocollagen, 3 parts of soluble collagen cross-linked polymer, 5 parts of hydrolyzed collagen zinc, 5 parts of stevioside and 60 parts of polyol; The molecular weight of the succinyl atelocollagen is 1500KD; and the polyol is 1,2-pentanediol.

3. An anti-wrinkle repairing composition, characterized in that: The invention comprises the following components in parts by weight: 5 parts of rice (ORYZA SATIVA) germ extract, 5 parts of olive (OLEA EUROPAEA) bud extract, 10 parts of LEPIDIUM SATIVUM bud extract, 3 parts of succinyl atelocollagen, 3 parts of soluble collagen cross-linked polymer, 3 parts of hydrolyzed collagen zinc, 3 parts of stevioside and 35 parts of polyol; The molecular weight of the succinyl atelocollagen is 1500KD; and the polyol is 1,2-pentanediol.

4. The method for preparing the anti-wrinkle repairing composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: (1) adding the polyol into a stirring pot, heating the pot to 30-42° C., and keeping the temperature for 10-15 minutes; (2) sequentially adding the succinyl atelocollagen, soluble collagen cross-linked polymer, hydrolyzed collagen zinc, stevioside, rice (ORYZA SATIVA) germ extract, olive (OLEA EUROPAEA) bud extract, and Lepidium SATIVUM bud extract into the polyol, stirring the mixture evenly, and obtaining the composition.

5. Use of the anti-wrinkle repairing composition according to any one of claims 1 to 3 in cosmetics, characterized in that: The cosmetics include lotions, creams, emulsions, facial masks and essences.

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