Polypeptide composite Pickering emulsion for efficient protein supplementation and preparation method and application thereof

Abstract

A polypeptide composite Pickering emulsion for efficient protein supplementation, a preparation method and an application thereof, belong to the technical field of emulsion preparation. In order to solve the technical problem of unbalanced protein content in the human body, the present invention provides a polypeptide composite Pickering emulsion for efficient protein supplementation and a preparation method thereof, wherein a polypeptide composite Pickering emulsion containing a pine mushroom extract is prepared by adding a pine mushroom extract, a polypeptide mixture, an amino acid mixture, a trace element mixture, corn germ oil, an embedded fatty acid, inulin, maltodextrin and β-cyclodextrin. The polypeptide composite Pickering emulsion provided by the present invention has the advantages of strong stability, high safety and low toxicity, and the polypeptide composite Pickering emulsion can be used to prepare a protein supplement medicament for quickly supplementing protein content, enhancing immunity and accelerating the recovery of various body skills.

Description

Technical Field

[0001] The invention belongs to the technical field of emulsion preparation, and relates to a Pickering emulsion and a preparation method and application thereof. Background Art

[0002] Protein is the material basis of life. Without protein, there is no life. It is a substance closely linked to life and various forms of life activities. Protein is involved in every cell and all important components of the body. Protein accounts for 16.3% of the body weight. There are many types of protein in the human body, with different properties and functions, but they are all composed of more than 20 kinds of amino acids in different proportions, and are constantly metabolized and renewed in the body. The ingested protein is digested and decomposed into amino acids in the body. After absorption, it is mainly used in the body to re-combine into human protein in a certain proportion. At the same time, the new protein is constantly metabolized and decomposed, and is always in dynamic balance. Therefore, the quality and quantity of food protein and the proportion of various amino acids are related to the amount of protein synthesis in the human body. In particular, the growth and development of adolescents, the eugenics of pregnant women, and the health and longevity of the elderly are closely related to the protein content in the human body.

[0003] Pickering emulsion is an emulsion stabilized only by solid particles located at the oil-water interface. The particles used to stabilize the emulsion are called Pickering particles or stabilizers. Compared with traditional emulsions, Pickering emulsions have the advantages of strong stability, high safety and low toxicity, and can be widely used in food, medicine and cosmetics. The development of environmentally friendly, naturally derived, renewable and biodegradable particles for stabilizing Pickering emulsions has gradually become a research hotspot. Researchers use a series of edible solid particles such as polysaccharides, proteins and small molecules to prepare stable food-grade Pickering emulsions through emulsification technologies such as high-speed homogenization, high-pressure homogenization and ultrasound, making them have the advantages of high biocompatibility, good stability and environmental friendliness. At the same time, it is applied to the transportation and preservation of bioactive substances in food processing, and the development of suitable loading and delivery systems will be of great significance for the efficient utilization of active substances. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a polypeptide composite Pickering emulsion for efficiently supplementing protein, as well as a preparation method and application thereof.

[0005] One of the purposes of the present invention is to provide a polypeptide composite Pickering emulsion for efficiently supplementing protein, wherein the polypeptide composite Pickering emulsion comprises the following components by weight: 5 parts of Matsutake extract, 140 parts of polypeptide mixture, 23 parts of amino acid mixture, 0.5 parts of trace element mixture, 1-30 parts of corn germ oil, 1-10 parts of embedded fatty acids, 50 parts of inulin, 20 parts of maltodextrin, and 3 parts of β-cyclodextrin.

[0006] In a preferred embodiment of the present invention, the polypeptide mixture comprises the following components in parts by weight: 100 parts of soy protein peptides, 20 parts of whey protein peptides and 20 parts of egg white peptides; the amino acid mixture comprises the following components in parts by weight: 20 parts of glutamine, 1 part of leucine, 1 part of isoleucine and 1 part of valine; the trace element mixture comprises the following components in parts by weight: 0.1 part of vitamin A, 0.1 part of calcium gluconate, 0.1 part of ferrous sulfate, 0.1 part of zinc sulfate and 0.1 part of taurine.

[0007] The second object of the present invention is to provide a method for preparing a polypeptide composite Pickering emulsion for efficiently supplementing protein. The method for preparing the polypeptide composite Pickering emulsion comprises the following steps:

[0008] S1: Select fresh matsutake mushrooms, wash and remove impurities, then slice or crush them to obtain matsutake mushroom slices / matsutake mushroom powder;

[0009] S2: putting the pine mushroom slices / pine mushroom powder obtained in S1 into an extraction container, adding an extraction solvent, stirring, and filtering the obtained pine mushroom extraction mixture after the extraction reaction is completed to obtain a pine mushroom extract;

[0010] S3: Concentrating the Tricholoma matsutake extract obtained in S2, drying the concentrated Tricholoma matsutake extract, and crushing the dried Tricholoma matsutake extract by a crusher to obtain Tricholoma matsutake extract powder;

[0011] S4: performing enzymatic hydrolysis on the soy protein peptide, whey protein peptide and egg white peptide, and after the enzymatic hydrolysis, performing separation and purification on the enzymatically hydrolyzed polypeptides, and then concentrating, drying and pulverizing to obtain a polypeptide mixture powder;

[0012] S5: The pine mushroom extract powder obtained in S3, the polypeptide mixture powder obtained in S4, the amino acid mixture, the trace element mixture, corn germ oil, embedded fatty acids, inulin, maltodextrin and β-cyclodextrin are uniformly mixed according to weight proportions to obtain a polypeptide composite Pickering emulsion.

[0013] In a preferred embodiment of the present invention, the extraction solvent in S2 is ethanol or water, the extraction temperature of the extraction reaction is ≤60°C, and the extraction time is 6 h-24 h.

[0014] In a preferred embodiment of the present invention, the filtration step described in S2 is: filtering the above-mentioned Tricholoma matsutake extract mixture using a sieve or filter cloth to separate the solid residue and the Tricholoma matsutake crude extract; using filter paper or a microporous filter membrane to perform a secondary filtration on the above-obtained Tricholoma matsutake crude extract.

[0015] In a preferred embodiment of the present invention, the concentration treatment in S3 is to evaporate the solvent at 30-60° C. using a rotary evaporator; and the drying treatment is to use a spray drying or freeze drying method.

[0016] In a preferred embodiment of the present invention, the enzymatic hydrolysis reaction described in S4 is to react the enzyme with soy protein peptide, whey protein peptide and egg white peptide in a mass addition ratio of 1: (20-60): (10-20): (10-20), and the enzymatic hydrolysis reaction is carried out under the conditions of 40-70°C and pH=8-10, and the reaction time of the enzymatic hydrolysis reaction is 3 h-7 h.

[0017] In a preferred embodiment of the present invention, the enzyme in the enzymatic hydrolysis reaction is any one of neutral protease, bromelain or alkaline protease.

[0018] In a preferred embodiment of the present invention, the separation and purification treatment steps described in S4 are: first centrifugation at 10000-15000×g and 4°C for 10-30 min, then dialysis using a membrane separation method, first removing suspended particles and macromolecular substances by microfiltration, and then controlling the parameters of the dialysis process, the flow rate is 1-1000 L / h, the pressure is 0.1-0.4 mPa, the temperature is 15-30°C, and the pH value is 6-8.

[0019] The third object of the present invention is to provide the use of the above-mentioned polypeptide composite Pickering emulsion for efficiently supplementing protein in the preparation of protein supplement medicine.

[0020] Beneficial effects of the present invention: Tricholoma matsutake, also known as pine mushroom, belongs to the genus Trichoderma of the family Trichoderma, the class Agaricales, the phylum Basidiomycetes, and its fruiting body is what we call the edible fungus "Matsutake". Matsutake contains rich nutrients and active substances, mainly including polysaccharides, steroids, saponins, oils, triterpenes, multiple essential amino acids and vitamins.

[0021] The present invention provides a polypeptide composite Pickering emulsion for efficiently supplementing protein, which is prepared by combining a matsutake extract, a polypeptide mixture, an amino acid mixture, a trace element mixture, corn germ oil, embedded fatty acids, inulin, maltodextrin and beta-cyclodextrin. In the process of preparing the matsutake extract, the present invention controls the extraction temperature below 60°C to prevent the loss of heat-sensitive components of the extract. In the process of enzymatic hydrolysis, the pH value and ionic strength of the enzymatic hydrolysis are controlled to maintain the activity of the enzyme and the stability of the protein.

[0022] The polypeptide composite Pickering emulsion for efficiently supplementing protein provided by the present invention has the function of reducing the contents of CRP, PCT, IL-6 and IL-4, thereby reducing inflammation caused by high contents of CRP, PCT, IL-6 and IL-4; the polypeptide composite Pickering emulsion can increase the number of blood albumin cells and the blood albumin concentration, thereby improving malnutrition caused by lack of blood albumin; the polypeptide composite Pickering emulsion can increase the concentration of prealbumin, thereby improving the transportation and distribution of thyroid hormone and vitamin A in the body; the polypeptide composite Pickering emulsion can increase the concentration of transferrin, thereby improving deficiency anemia or iron overload caused by lack of transferrin; the polypeptide composite Pickering emulsion can increase the concentration of hemoglobin, thereby improving anemia caused by lack of hemoglobin.

[0023] The polypeptide composite Pickering emulsion for efficiently supplementing protein provided by the present invention has higher safety compared with traditional emulsions and avoids the use of toxic surfactants; the polypeptide composite Pickering emulsion has strong stability and is not easily affected by factors such as system pH value, salt concentration, temperature and oil phase composition; the polypeptide composite Pickering emulsion can be used to prepare protein supplement agents for quickly supplementing protein content, enhancing immunity, and accelerating the recovery of various body skills. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a graph showing the results of the C-reactive protein content test in the blood sample to be tested;

[0025] Figure 2 This is a graph showing the results of the procalcitonin content test in the blood sample to be tested;

[0026] Figure 3 This is a graph showing the results of the interleukin-6 content test in the blood sample to be tested;

[0027] Figure 4 This is a graph showing the results of the interleukin-4 content test in the blood sample to be tested;

[0028] Figure 5 It is a statistical chart of the number of white blood cells in the blood sample to be tested;

[0029] Figure 6 A statistical diagram of the number of red blood cells in the blood sample to be tested;

[0030] Figure 7 It is a graph showing the test result of the blood albumin concentration in the blood sample to be tested;

[0031] Figure 8 This is a graph showing the test results of the concentration of prealbumin in the blood sample to be tested;

[0032] Fig. 9 This is a graph showing the test result of transferrin concentration in the blood sample to be tested;

[0033] Fig.10 This is a graph showing the hemoglobin concentration test results in the blood sample to be tested. DETAILED DESCRIPTION

[0034] Those skilled in the art can refer to the content of this article and appropriately improve the process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0035] In order to make the purpose, technical scheme and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific implementation methods and the accompanying drawings of the specification. The experimental methods used in the following examples are conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and can be obtained by those skilled in the art through commercial channels.

[0036] Example 1

[0037] S1: Select fresh matsutake mushrooms, wash and remove impurities, then slice or crush them to obtain matsutake mushroom slices / matsutake mushroom powder;

[0038] S2: Put the Tricholoma matsutake slices / powder obtained in S1 into an extraction container, add water extraction solvent, stir, and the extraction temperature of the extraction reaction is ≤60°C, and the extraction time is 12 h. After the extraction reaction is completed, filter the Tricholoma matsutake extraction mixture using a sieve or filter cloth to separate the solid residue and the Tricholoma matsutake crude extract; use filter paper or a microporous filter membrane to perform a secondary filtration on the Tricholoma matsutake crude extract obtained above to obtain a Tricholoma matsutake extract;

[0039] S3: placing the Tricholoma matsutake extract obtained in S2 in a rotary evaporator, evaporating and concentrating the solvent at 45° C., drying the concentrated Tricholoma matsutake extract by spray drying or freeze drying, and crushing the dried Tricholoma matsutake extract by a grinder to obtain Tricholoma matsutake extract powder;

[0040] S4: performing an enzymatic hydrolysis reaction on soybean protein peptide, whey protein peptide and egg white peptide, wherein the enzymatic hydrolysis reaction is to react neutral protease with soybean protein peptide, whey protein peptide and egg white peptide in a mass addition ratio of 1:40:10:10, and performing the enzymatic hydrolysis reaction at 37°C and pH=8, and the reaction time of the enzymatic hydrolysis reaction is 6 h; then centrifuging the polypeptide mixture after the enzymatic hydrolysis reaction at 10000-15000×g and 4°C for 15 min, dialyzing the polypeptide mixture after the centrifugation by a membrane separation method, first removing suspended particles and macromolecular substances by microfiltration, and then controlling the parameters in the dialysis process, the flow rate is 1-1000 L / h, the pressure is 0.1-0.4 mPa, the temperature is 15-30°C, and the pH value is 6-8, to obtain a separated and purified polypeptide mixture, and then concentrating, drying and pulverizing to obtain a polypeptide mixture powder;

[0041] S5: The Matsutake extract powder obtained in S3, the polypeptide mixture powder obtained in S4, the amino acid mixture, the trace element mixture, corn germ oil, embedded fatty acids, inulin, maltodextrin and β-cyclodextrin are uniformly mixed in a certain addition ratio to obtain a polypeptide composite Pickering emulsion for efficient protein supplementation.

[0042] The polypeptide composite Pickering emulsion for efficient protein supplementation prepared in this embodiment includes the following components by weight: 5 parts of Matsutake extract, 140 parts of polypeptide mixture, 23 parts of amino acid mixture, 0.5 parts of trace element mixture, 3 parts of corn germ oil, 2 parts of embedded fatty acid, 50 parts of inulin, 20 parts of maltodextrin, and 3 parts of β-cyclodextrin.

[0043] The polypeptide mixture comprises the following components in parts by weight: 100 parts of soybean protein peptide, 20 parts of whey protein peptide and 20 parts of egg white peptide; the amino acid mixture comprises the following components in parts by weight: 20 parts of glutamine, 1 part of leucine, 1 part of isoleucine and 1 part of valine; the trace element mixture comprises the following components in parts by weight: 0.1 part of vitamin A, 0.1 part of calcium gluconate, 0.1 part of ferrous sulfate, 0.1 part of zinc sulfate and 0.1 part of taurine.

[0044] Comparative Example 1

[0045] S1: performing an enzymatic hydrolysis reaction on soybean protein peptide, whey protein peptide and egg white peptide, wherein the enzymatic hydrolysis reaction is to react neutral protease with soybean protein peptide, whey protein peptide and egg white peptide in a mass addition ratio of 1:40:10:10, and performing the enzymatic hydrolysis reaction at 37°C and pH=8, and the reaction time of the enzymatic hydrolysis reaction is 6 h; then centrifuging the polypeptide mixture after the enzymatic hydrolysis reaction at 10000-15000×g and 4°C for 15 min, dialyzing the polypeptide mixture after the centrifugation by a membrane separation method, first removing suspended particles and macromolecular substances by microfiltration, and then controlling the parameters in the dialysis process, the flow rate is 1-1000 L / h, the pressure is 0.1-0.4 mPa, the temperature is 15-30°C, and the pH value is 6-8, to obtain a separated and purified polypeptide mixture, and then concentrating, drying and pulverizing to obtain a polypeptide mixture powder;

[0046] S2: The polypeptide mixture powder, amino acid mixture, trace element mixture, corn germ oil, embedded fatty acid, inulin, maltodextrin and β-cyclodextrin obtained in S1 are uniformly mixed according to a certain addition ratio to obtain a polypeptide composite Pickering emulsion.

[0047] The polypeptide composite Pickering emulsion for efficient protein supplementation prepared in this embodiment includes the following components: 140 parts of polypeptide mixture, 23 parts of amino acid mixture, 0.5 parts of trace element mixture, 12 parts of corn germ oil, 2 parts of embedded fatty acid, 50 parts of inulin, 20 parts of maltodextrin, and 3 parts of β-cyclodextrin.

[0048] The polypeptide mixture includes 100 parts of soy protein peptides, 20 parts of whey protein peptides and 20 parts of egg white peptides; the amino acid mixture includes 20 parts of glutamine, 1 part of leucine, 1 part of isoleucine and 1 part of valine; the trace element mixture includes 0.1 part of vitamin A, 0.1 part of calcium gluconate, 0.1 part of ferrous sulfate, 0.1 part of zinc sulfate and 0.1 part of taurine.

[0049] Effect experiment:

[0050] The present invention selects 40 volunteers, and the experimental groups take the polypeptide composite Pickering emulsion for efficient protein supplementation prepared in Example 1, respectively, and are divided into a low-dose taking group of 100 ml / day and a high-dose taking group of 400 ml / day; the control group takes the polypeptide composite Pickering emulsion without Matsutake extract prepared in Comparative Example 1, and the taking dosage is 100 ml / day, and blood is sampled from the volunteers after taking the emulsion for 14 days to obtain blood samples to be tested.

[0051] The Human C-Reactive Protein ELISA Kit (enzyme-linked immunosorbent assay) of MULTI SCIENCES was used to perform mass spectrometry analysis on the contents of C-reactive protein (CRP), procalcitonin (PCT), interleukin 6 (IL-6) and interleukin 4 (IL-4) in the above-obtained test samples. For specific operation steps, please refer to the kit instructions.

[0052] Result analysis:

[0053] like Figure 1 As shown in Table 1, compared with the blood samples of the control group, the CRP content in the blood samples of the low / high-dose experimental group was significantly reduced; and the CRP content in the blood samples of the high-dose polypeptide composite Pickering emulsion was lower than that of the low-dose polypeptide composite Pickering emulsion; the normal range of CRP content is generally less than 10 mg / L, and the polypeptide composite Pickering emulsion provided in the present application can effectively reduce the function of CRP content; among them, the test results of the high-dose Pickering emulsion supplementation group showed that the CRP content had been reduced to a normal range.

[0054] Table 1

[0055]

[0056] like Figure 2 As shown in Table 2, compared with the blood samples of the control group, the PCT content in the blood samples of the low / high-dose experimental group was significantly reduced; and the PCT content in the blood samples of the high-dose polypeptide composite Pickering emulsion was lower than that of the low-dose polypeptide composite Pickering emulsion; the normal range of PCT content is generally less than 0.1 ng / mL, and the polypeptide composite Pickering emulsion provided in the present application can effectively reduce the function of PCT content; among them, the test results of the high-dose Pickering emulsion supplementation group showed that the PCT content had been reduced to the normal range.

[0057] Table 2

[0058]

[0059] like Figure 3-4 As shown in Tables 3-4, compared with the blood samples of the control group, the IL-6 and IL-4 levels in the blood samples of the low / high-dose experimental groups were significantly reduced; and, the IL-6 and IL-4 levels in the blood samples of the high-dose polypeptide compound Pickering emulsion were lower than those of the low-dose polypeptide compound Pickering emulsion; the normal range of IL-6 and IL-4 levels is generally less than 5 pg / mL, and the polypeptide compound Pickering emulsion provided in the present application can effectively reduce the IL-6 and IL-4 levels, and reduce the IL-6 and IL-4 levels to within the normal range.

[0060] Table 3

[0061]

[0062] Table 4

[0063]

[0064] The polypeptide composite Pickering emulsion provided by the present invention has the function of reducing the contents of CRP, PCT, IL-6 and IL-4 at the same time, and has the effect of reducing inflammation caused by high contents of CRP, PCT, IL-6 and IL-4.

[0065] like Figure 5-6 As shown in Tables 5-6, compared with the blood samples of the control group, the numbers of white blood cells and red blood cells in the blood samples of the low / high-dose experimental groups were increased; and the numbers of white blood cells and red blood cells in the blood samples of the high-dose polypeptide composite Pickering emulsion were higher than those of the low-dose polypeptide composite Pickering emulsion; it can be seen that the polypeptide composite Pickering emulsion provided in the present application can effectively increase the number of white blood cells and red blood cells.

[0066] Table 5

[0067]

[0068] Table 6

[0069]

[0070] like Figure 7-8 As shown in Tables 7-8, compared with the blood samples of the control group, the prealbumin concentration in the blood samples of the low / high-dose experimental group was significantly increased, and the blood albumin concentration was increased; and the blood albumin and prealbumin concentrations in the blood samples of the high-dose polypeptide composite Pickering emulsion were higher than those of the low-dose polypeptide composite Pickering emulsion; it can be seen that the polypeptide composite Pickering emulsion provided by the present application can effectively increase the concentrations of blood albumin and prealbumin, improve malnutrition caused by lack of blood albumin, and improve the transport and distribution of thyroid hormone and vitamin A in the body.

[0071] Table 7

[0072]

[0073] Table 8

[0074]

[0075] like Fig. 9As shown in Table 9, compared with the blood samples of the control group, the transferrin concentration in the blood samples of the low / high-dose experimental group was increased, among which the difference in transferrin concentration in the blood samples of the high-dose and low-dose polypeptide composite Pickering emulsions was not significant; it can be seen that the polypeptide composite Pickering emulsion provided by the present application can increase the transferrin concentration, but the increase in the dosage has no significant effect on the increase in transferrin concentration, and has the effect of improving deficiency anemia or iron overload caused by lack of transferrin.

[0076] Table 9

[0077]

[0078] like Fig.10 As shown in Table 10, compared with the blood samples of the control group, the hemoglobin concentration in the blood samples of the low / high dose experimental group was increased, and the hemoglobin concentration in the blood samples of the low dose polypeptide composite Pickering emulsion was not significantly different from that of the control group; it can be seen that the polypeptide composite Pickering emulsion provided by the present application can increase the hemoglobin concentration, but the effect of increasing the hemoglobin concentration is not significant when the dosage is low. When the dosage is 400 ml / day, it can fully exert the function of increasing the hemoglobin concentration, and has the effect of improving anemia caused by lack of hemoglobin.

[0079] Table 10

[0080]

[0081] The contents not described in detail in the specification of the present invention are well-known technologies for those skilled in the art. Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with the technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be based on the definition of the claims.

Claims

1. A polypeptide composite Pickering emulsion for efficient protein supplementation, characterized in that: The polypeptide composite Pickering emulsion is composed of the following ingredients by weight: 5 parts of Matsutake extract, 140 parts of polypeptide mixture, 23 parts of amino acid mixture, 0.5 parts of trace element mixture, 1-30 parts of corn germ oil, 1-10 parts of embedded fatty acids, 50 parts of inulin, 20 parts of maltodextrin, and 3 parts of β-cyclodextrin; The polypeptide mixture consists of the following ingredients by weight: 100 parts of soybean protein peptide, 20 parts of whey protein peptide and 20 parts of egg white peptide; The amino acid mixture consists of the following components by weight: 20 parts of glutamine, 1 part of leucine, 1 part of isoleucine and 1 part of valine; The trace element mixture consists of the following components by weight: 0.1 part of vitamin A, 0.1 part of calcium gluconate, 0.1 part of ferrous sulfate, 0.1 part of zinc sulfate and 0.1 part of taurine; The preparation method of the polypeptide composite Pickering emulsion comprises the following steps: S1: Select fresh matsutake mushrooms, wash and remove impurities, and then crush them to obtain matsutake mushroom powder; S2: putting the pine mushroom powder obtained in S1 into an extraction container, adding an extraction solvent, stirring, and filtering the obtained pine mushroom extraction mixture after the extraction reaction is completed to obtain a pine mushroom extract; S3: Concentrating the Tricholoma matsutake extract obtained in S2, drying the concentrated Tricholoma matsutake extract, and crushing the dried Tricholoma matsutake extract by a crusher to obtain Tricholoma matsutake extract powder; S4: performing enzymatic hydrolysis on the soy protein peptide, whey protein peptide and egg white peptide, and after the enzymatic hydrolysis, performing separation and purification on the enzymatically hydrolyzed polypeptides, and then concentrating, drying and pulverizing to obtain a polypeptide mixture powder; S5: the pine mushroom extract powder obtained in S3, the polypeptide mixture powder obtained in S4, the amino acid mixture, the trace element mixture, corn germ oil, the embedded fatty acid, inulin, maltodextrin and β-cyclodextrin are uniformly mixed according to weight proportions to obtain a polypeptide composite Pickering emulsion for efficient protein supplementation; The extraction solvent in S2 is ethanol or water; the extraction temperature of the extraction reaction is ≤60°C, and the extraction time is 6h-24h; The filtering step in S2 is: filtering the Tricholoma matsutake extract mixture using a sieve or filter cloth to separate solid residues and Tricholoma matsutake crude extract; and performing secondary filtering on the Tricholoma matsutake crude extract obtained using filter paper or a microporous filter membrane; The enzymatic hydrolysis reaction described in S4 is to react the enzyme with soy protein peptide, whey protein peptide and egg white peptide in a mass addition ratio of 1: (20-60): (10-20): (10-20), and the enzymatic hydrolysis reaction is carried out under the conditions of 40-70°C and pH=8-10. The reaction time of the enzymatic hydrolysis reaction is 3 h-7 h; the enzyme in the enzymatic hydrolysis reaction is any one of neutral protease, bromelain or alkaline protease.

2. The polypeptide composite Pickering emulsion according to claim 1, characterized in that: The concentration step in the preparation method S3 of the polypeptide composite Pickering emulsion is: evaporating the solvent at 30-60° C. using a rotary evaporator; and the drying is any one of spray drying and freeze drying.

3. The polypeptide composite Pickering emulsion according to claim 1, characterized in that: The steps of separation and purification treatment described in the preparation method S4 of the polypeptide composite Pickering emulsion are: centrifugation at 10000-15000×g and 4°C for 10-30min, and then dialysis using a membrane separation method, first removing suspended particles and macromolecular substances by microfiltration, and then controlling the parameters during the dialysis process, with a flow rate of 1-1000 L / h, a pressure of 0.1-0.4 mPa, a temperature of 15-30°C, and a pH value of 6-8.

4. Use of the polypeptide composite Pickering emulsion for efficient protein supplementation according to any one of claims 1 to 3 in the preparation of protein supplementation agents.

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