Modular multiplexed detection device and method of detection thereof

Abstract

The application particularly relates to a modular multi-detection device for biochemical reactions and a detection method thereof. The device comprises one or more detection modules, each detection module comprising a cavity for configuring a reaction container, a plurality of temperature control components and a plurality of optical components corresponding to the reaction container, and a signal processing circuit, wherein the reaction container is a container for nucleic acid amplification reaction, and part or all of the plurality of optical components are symmetrically arranged around the reaction container in the same plane; and a master control module comprising a control circuit for sending control signals to each detection module and receiving feedback signals from each detection module. The application can realize modularization, miniaturization and structural standardization of the detection device with multi-amplification and multi-detection capabilities, has high expansibility, can customize instruments with different detection fluxes according to needs and application scenarios, has a short instrument development cycle, and is low in production cost.

Description

TECHNICAL FIELD

[0001] The present application relates to biochemical reactions, in particular to a modular multi-detection device for biochemical reactions and a detection method thereof. BACKGROUND

[0002] Some amplification devices have been developed to amplify target molecules. In addition, some detection devices have also been developed to detect target molecules. However, the existing integrated amplification-detection devices are bulky and need to be used in specialized laboratories; the associated optical and temperature control structures are complex when involving multiple amplification and detection, poor scalability, and high cost.

[0003] Therefore, there is an urgent need for a detection scheme to realize the modularization, miniaturization and structural standardization of detection devices with multiple amplification and detection capabilities, high scalability, and customization of instruments with multiple amplification and detection capabilities of different detection throughput according to needs and application scenarios, short instrument development cycle, and low production cost. SUMMARY

[0004] In order to solve the above problems in the prior art, the present application provides a modular multi-detection device for biochemical reactions and a detection method thereof, which can realize the modularization, miniaturization and structural standardization of detection devices with multiple amplification and detection capabilities, high scalability, and customization of instruments with multiple amplification and detection capabilities of different detection throughput according to needs and application scenarios, short instrument development cycle, and low production cost.

[0005] The present application provides a modular multi-detection device, which comprises:

[0006] one or more detection modules, each detection module comprising a cavity for configuring a reaction container, a plurality of temperature control components and a plurality of optical components corresponding to the reaction container, and a signal processing circuit, wherein the reaction container is a container for one or more nucleic acid amplification reactions, the plurality of temperature control components are used to control the one or more nucleic acid amplification reactions in the reaction container, the plurality of optical components are used to detect one or more types of fluorescent signals generated by the one or more nucleic acid amplification reactions, and part or all of the plurality of optical components are symmetrically arranged around the reaction container on the same plane;

[0007] a master control module, the master control module comprising a control circuit for sending control signals to each detection module and receiving feedback signals from each detection module.

[0008] In one embodiment of the present application, each temperature control assembly includes a first heater and a second heater, the first heater is located at a first end of the reaction vessel, and the second heater is located at a second end of the reaction vessel opposite to the first end.

[0009] In one embodiment of the present application, the control signal includes a temperature control signal, and the output power of the first heater and the second heater is controlled based on the temperature control signal to conduct heat to the reaction vessel and the reaction solution inside the reaction vessel.

[0010] In one embodiment of the present application, each temperature control assembly further includes a first cooler and a second cooler, the first cooler is located at the first end, and the second cooler is located at the second end, and the output power of the first cooler and the second cooler is controlled based on the temperature control signal to absorb heat from the reaction vessel and the reaction solution inside the reaction vessel.

[0011] In one embodiment of the present application, the cooler is a fan or a semiconductor cooling device.

[0012] In one embodiment of the present application, each temperature control assembly further includes a first temperature sensor and a second temperature sensor, the first temperature sensor and the second temperature sensor are respectively used to determine the temperature at the first end and the second end as a temperature feedback signal fed back to the control circuit.

[0013] In one embodiment of the present application, each optical assembly includes a light source assembly and a light sensing assembly, the light source assembly includes a light emitter used to emit excitation light and irradiate the reaction solution inside the reaction vessel, and the light sensing assembly includes a light receiver used to detect a fluorescent signal generated by the reaction solution and convert the detected fluorescent signal into an electrical signal.

[0014] Under the action of the plurality of temperature control assemblies, the one or more nucleic acid amplification reactions in the reaction vessel can generate one or more free fluorescent dyes specific to one or more target molecules and associated with the amount of the one or more target molecules, under the action of the plurality of optical assemblies, one or more types of fluorescent signals generated by the one or more free fluorescent dyes excited are not quenched and detected, so that multiple types of fluorescent signals can be detected simultaneously from one reaction vessel to achieve multiplex detection.

[0015] In one embodiment of the present application, the control signal includes a detection control signal, and the emission time of the light emitter and one or more of the light intensity and the light wavelength of the excitation light are controlled based on the detection control signal.

[0016] In one embodiment of the present application, the light source assembly further comprises an excitation light filter and an excitation light guide medium, and the light sensing assembly further comprises a fluorescent light filter and a fluorescent light guide medium.

[0017] In one embodiment of the present application, the light guide medium is a solid transparent material, including silicon-based materials (such as glass and quartz, etc.) and optical plastics (such as polymethyl methacrylate (PMMA), polystyrene (PS), polycarbonate (PC), propylene glycol carbonate (CR39), styrene-acrylonitrile copolymer (SAN), styrene-acrylate copolymer (NAS), poly 4-methyl pentene 1 (TPX), etc.), etc., the ratio of the length to the diameter or the longest diagonal of the light guide medium is not greater than 50, and the average distance between one end of the light guide medium and the light emitter or the light receiver and the other end of the light guide medium and the surface of the reaction container is less than 5 mm.

[0018] In one embodiment of the present application, the plurality of light source assemblies and the plurality of light sensing assemblies are symmetrically arranged around the reaction container on the same plane, or

[0019] The plurality of light source assemblies and the plurality of light sensing assemblies are not arranged on the same plane, wherein the plurality of light sensing assemblies are symmetrically arranged around the reaction container on the same plane and the plurality of light source assemblies are arranged at one end of the reaction container, i.e. the lower end or the upper end, or wherein the plurality of light source assemblies are symmetrically arranged around the reaction container on the same plane and the plurality of light sensing assemblies are arranged at one end of the reaction container, i.e. the lower end or the upper end.

[0020] In one embodiment of the present application, the plurality of light source assemblies and the plurality of light sensing assemblies are paired one by one, the wavelength of the excitation light filter of each light source assembly is the same or different, and the wavelength of the fluorescent light filter of each light sensing assembly is the same or different.

[0021] The plurality of light source assemblies and the plurality of light sensing assemblies can be paired one by one according to the characteristics of the fluorescent dyes to be detected, when the fluorescent signal of the fluorescent dye is not quenched, different light source assembly-light sensing assembly pairs can detect the fluorescent signal of different fluorescent dyes, and multiple light source assembly-light sensing assembly pairs can simultaneously detect multiple fluorescent signals of multiple fluorescent dyes.

[0022] In one embodiment of the present application, the number of the plurality of optical assemblies is 2 to 10.

[0023] In one embodiment of the present application, the number of the plurality of optical assemblies is 4.

[0024] In one embodiment of the present invention, when the multiple light source assemblies or the multiple photosensitive assemblies are arranged at one end of the reaction container, the photoconductive medium of the multiple light source assemblies or the multiple photosensitive assemblies forms a pyramid or cone-shaped structure, that is, the multiple light emitters or the multiple light receivers are respectively gathered together to one end of the reaction container along an inner inclination angle (less than 90 degrees but greater than 45 degrees) through the photoconductive medium.

[0025] In one embodiment of the present invention, the signal processing circuit is used to amplify and perform analog-to-digital conversion on the electrical signal converted by the optical receiver so as to be fed back to the control circuit as a detection feedback signal, wherein the electrical signal is typically an analog signal and the detection feedback signal is typically a digital signal.

[0026] In one embodiment of the present invention, each detection module further includes a communication interface, and the main control module further includes a communication circuit, and each detection module and the main control module communicate with each other via the communication interface and the communication circuit.

[0027] In one embodiment of the present invention, the control circuit includes a memory and a processor, the memory is used to store software instructions, and the processor is used to execute the software instructions.

[0028] In one embodiment of the present invention, the main control module further includes a display circuit, and the display circuit is used to display the detection results of each detection module.

[0029] In one embodiment of the present invention, each detection module further includes a module housing.

[0030] In one embodiment of the present invention, the number of the one or more detection modules is 1, 2 to 10, or 2 to 100.

[0031] The present invention further provides a detection method for the modular multiple detection device described above, the method comprising:

[0032] Arrange a reaction container filled with a reaction solution into a cavity of a corresponding detection module, wherein the reaction solution includes a reaction system and a detection sample, and the detection sample contains one or more target molecules to be detected;

[0033] The control circuit of the main control module sends a temperature control signal to each detection module configured with the reaction container, so as to control the multiple temperature control components of each detection module to transfer heat to the corresponding reaction container and the reaction solution therein, so that the reaction solution can form heat convection;

[0034] sending, by the control circuit, a detection control signal to each detection module to control a light source component in a plurality of optical components of each detection module to emit excitation light and irradiate the reaction solution inside the corresponding reaction container;

[0035] detecting, by a photosensitive component in the plurality of optical components of each detection module, one or more types of fluorescent signals generated by the reaction solution inside the corresponding reaction container, so that a signal processing circuit of each detection module can generate one or more detection feedback signals;

[0036] receiving, by the control circuit, the one or more detection feedback signals from each detection module.

[0037] Under the action of the plurality of temperature control components, one or more nucleic acid amplification reactions in the reaction containers can generate one or more free fluorescent dyes specific to the one or more target molecules and associated with the amount of the one or more target molecules, and under the action of the plurality of optical components, one or more types of fluorescent signals generated by the one or more free fluorescent dyes being excited are not quenched and detected, so that multiple types of fluorescent signals can be detected simultaneously from one reaction container to achieve multiplex detection.

[0038] In one embodiment of the present application, the method further comprises:

[0039] determining, by the plurality of temperature control components of each detection module, the temperature at a plurality of heating points of the corresponding reaction container, so that each detection module can generate a plurality of temperature feedback signals;

[0040] receiving, by the control circuit, the plurality of temperature feedback signals from each detection module.

[0041] In one embodiment of the present application, the target molecule includes one or more of an RNA molecule or a DNA molecule, an RNA fragment in an RNA genome or a DNA fragment in a DNA genome, and a variant structure in an RNA molecule or a DNA molecule.

[0042] In one embodiment of the present application, the target molecule is derived from a human, an animal, a plant, a microorganism, or artificially or chemically synthesized, wherein the microorganism includes one or more of a virus, a bacterium, and a fungus.

[0043] In one embodiment of the present application, the reaction system includes primers for one or more nucleic acid amplification reactions, one or more detection probes for detecting the one or more target molecules, and a DNA polymerase, wherein each detection probe is provided with a fluorescent dye corresponding to the corresponding target molecule.

[0044] In one embodiment of the present application, the DNA polymerase has 3'→5' exonuclease activity.

[0045] As described above, the present application has the following beneficial effects:

[0046] The detection module in the present application has an independent and complete temperature control and optical structure design, that is, it has a plurality of temperature control components, a plurality of optical components (including a light source component and a photosensitive component), a signal processing circuit and a communication interface. Each detection module can be controlled by a master control module and can simultaneously detect one or more types of fluorescent signals generated by one or more nucleic acid amplification reactions. One master control module can control one or more detection modules, such as 1 or 2 to 10 or 2 to 100 detection modules, that is, one detection device can simultaneously or separately detect one or more types of fluorescent signals of 1 or 2 to 10 or 2 to 100 samples, realizing the modularization, combination, miniaturization and structural standardization of the detection device, high expansibility and low production cost. In addition, compared with the existing detection device, the detection device of the present application can obtain multiple detection results more quickly in a smaller volume in various application scenarios (such as bedside detection, on-site detection (POCT)).

[0047] Since the detection module in the present application is an independent structure with a dedicated temperature control component, a plurality of optical components (including a light source component and a photosensitive component), a signal processing circuit and a communication interface, the detection module can be freely combined, and each detection module can share a master control module and be independently controlled by the master control module without being affected by other detection modules, so that instruments with multiple amplification and multiple detection capabilities of different detection fluxes can be customized according to needs and application scenarios, the instrument development cycle is short, and the production cost is low. BRIEF DESCRIPTION OF DRAWINGS

[0048] Figure 1 is a functional module schematic diagram of a single-module multiple detection device according to an embodiment of the present application;

[0049] Figure 2A and Figure 2B are, respectively, a front view and a top view of a functional component of a detection module according to an embodiment of the present application;

[0050] Figure 3 is another front view of a functional component of a detection module according to an embodiment of the present application;

[0051] Figures 4A to 4C are, respectively, a front view, a front view and a top view of a mounting structure of a detection module according to an embodiment of the present application;

[0052] Figure 5 is a front view of a mounting structure of a light source component and a photosensitive component according to an embodiment of the present application;

[0053] Figure 6 is a temperature feedback signal plot of a single-module multiplex detection device according to an embodiment of the present application;

[0054] Figure 7 is a detection feedback signal plot of a single-module multiplex detection device according to an embodiment of the present application;

[0055] Figure 8 is a partial schematic diagram of a multi-module multiplex detection device according to an embodiment of the present application;

[0056] Figure 9 is a communication schematic diagram of a multi-module multiplex detection device according to an embodiment of the present application. DETAILED DESCRIPTION

[0057] Embodiments of the present application are described herein according to the following figures.

[0058] Exemplary embodiments are described herein below with reference to the accompanying drawings.

[0059] The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in the present application and the appended claims, the singular forms "a," "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will also be understood that the term "or" as used herein refers to a non-exclusive "or," unless otherwise indicated. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof. It will be understood that the terms "evenly" and "uniformly" as used herein refer to a substantially uniform distribution, unless otherwise indicated.

[0060] First embodiment

[0061] A first embodiment of the present application provides a single-module multiplex detection device.

[0062] Figure 1is a functional module schematic diagram of a single-module multiplex detection device according to an embodiment of the present application.

[0063] As shown in Figure 1 , the single-module multiplex detection device includes a detection module 100 and a master control module 200.

[0064] The detection module 100 includes a cavity 101 for configuring a reaction container 301, and a temperature control assembly 102 and optical assemblies 103, 104 corresponding to the reaction container 301.

[0065] It can be understood that the detection module 100 can include one or more cavities 101, and each cavity 101 can be used to configure one or more reaction containers 301; for example, the detection module 100 includes one cavity 101 for configuring one reaction container 301; for another example, the detection module 100 includes one cavity 101 for configuring multiple reaction containers 301; for yet another example, the detection module 100 includes multiple cavities 101, and each cavity 101 is used to configure one reaction container 301; for the convenience of description, Figure 1 only one cavity 101 for configuring one reaction container 301 is shown. The reaction container 301 is not specific to the detection module 100, and can be replaced in different detection reactions.

[0066] For each reaction container 301, the detection module 100 is provided with an independent temperature control assembly 102 and optical assemblies 103, 104. In other words, in the case that the detection module 100 is configured with multiple reaction containers 301, these reaction containers 301 do not share the temperature control assembly 102 and the optical assemblies 103, 104. For the convenience of description, Figure 1 only one temperature control assembly 102 and one optical assembly 103, 104 are shown, but as will be described below, each reaction container 301 can be provided with multiple temperature control assemblies 102 and multiple optical assemblies 103, 104 to achieve the effect of multiplex detection.

[0067] The reaction container 301 can also be referred to as a reaction test tube, and the reaction container 301 can provide a reaction space, which can also be referred to as a reaction cavity. A reaction solution can be injected into the reaction space of the reaction container 301, and the reaction solution can include a reaction system and a detection sample, and the detection sample can have one or more target molecules to be detected.

[0068] The reaction container 301 can be a container for one or more nucleic acid amplification reactions, the plurality of temperature control components 102 can be used to control the one or more nucleic acid amplification reactions in the reaction container 301, and the plurality of optical components 103, 104 can be used to detect one or more types of fluorescent signals generated by the one or more nucleic acid amplification reactions. The temperature control components 102 can be controlled according to a certain procedure to perform heating, cooling, and constant temperature control, so that the temperature control components 102 can conduct heat to the reaction container 301 and the reaction solution inside the reaction container 301, and so that one or more target molecules can perform one or more nucleic acid amplification reactions under the catalysis of enzymes in the reaction system to generate one or more free fluorescent dyes specific to the one or more target molecules and related to the amount of the one or more target molecules. The plurality of optical components 103, 104 can detect one or more types of fluorescent signals generated by the excitation of the one or more free fluorescent dyes, so that multiple types of fluorescent signals can be detected simultaneously in one reaction container 301 to achieve multiplex detection.

[0069] The optical components 103, 104 include a light source component 103 and a light sensing component 104. The light source component 103 can be controlled according to a certain procedure to perform lighting and turning off, so that the light source component 103 can emit excitation light and irradiate the reaction solution inside the reaction container 301, and so that the light sensing component 104 can detect the fluorescent signals generated by the reaction solution (for example, by using a fluorescent probe method). By analyzing the signal characteristics (such as intensity level, emission wavelength, and emission regularity) of the fluorescent signals during the reaction process, the entire process of the solution reaction can be understood, and the type of the target molecule can be determined.

[0070] The control module 200 includes a control circuit 201. The control circuit 201 can be used to send a control signal to the detection module 100, which can include a temperature control signal for controlling the temperature control components 102 and a detection control signal for controlling the light source component 103. The control circuit 201 can receive a feedback signal from the detection module 100, which can include a temperature feedback signal indicating the temperature at the heating point of the reaction container 301 and a detection feedback signal indicating the signal characteristics of the fluorescent signals.

[0071] In one embodiment, the control circuit 201 can include a memory for storing software instructions and a processor for executing the software instructions. For example, the processor can be an integrated circuit processor MCU, and the memory can be part of the integrated circuit processor MCU.

[0072] In one embodiment, each detection module 100 can also include a module support or housing (not shown), so as to provide protection for the detection module 100 and to achieve modularization of the detection device.

[0073] Figure 2A and Figure 2B They are respectively a front view and a top view of the functional components of a detection module according to an embodiment of the present invention.

[0074] like Figure 2A As shown, in one embodiment, the reaction vessel 301 may have a first end and a second end opposite to the first end, wherein the first end of the reaction vessel 301 may be an open end, and the second end of the reaction vessel 301 may be a closed end. The reaction vessel 301 may be a tubular structure, such as a round tubular structure or a square tubular structure. Compared with a sheet-like reaction vessel, a tubular reaction vessel is easier to package. The first end and the second end of the reaction vessel 301 may be arranged relative to each other in the longitudinal direction of the tubular structure. Figure 2A In the embodiment, the first end of the reaction vessel 301 may also be referred to as the upper end of the tubular structure, and the second end of the reaction vessel 301 may also be referred to as the lower end of the tubular structure.

[0075] In one embodiment, the reaction vessel 301 may be made of a transparent material, which may be suitable for the fluorescence signal detection process.

[0076] In one embodiment, the temperature control assembly 102 may include a first heater 1021 and a second heater 1022 , the first heater 1021 may be located at a first end of the reaction vessel 301 , and the second heater 1022 may be located at a second end of the reaction vessel 301 .

[0077] The output power of the first heater 1021 and the second heater 1022 can be controlled based on the temperature control signal as described above, to the reaction vessel 301 and the reaction solution conduction heat thereof. The operating state of the first heater 1021 and the second heater 1022 can include heating, cooling and constant temperature etc., so that it is possible to realize nucleic acid target molecule amplification. According to different reaction solutions, the output power of the first heater 1021 and the second heater 1022 can be different, thereby can realize different detection reactions. In addition, according to the requirement of reaction, the temperature at the first end and the second end as heating point can be different, thereby can form a temperature gradient between the first end and the second end, so that the reaction solution of the inside of reaction tube 301 can form thermal convection.

[0078] In one embodiment, the temperature control assembly 102 can further include a first cooler and a second cooler (not shown), the first cooler is located at the first end and the second cooler is located at the second end. The output power of the first cooler and the second cooler can be controlled based on the temperature control signal as described above to absorb the heat from the reaction vessel 301 and the reaction solution inside it so that the detection module 100 can be cooled down when it is overheated. As an example, the first cooler and the second cooler can be a fan or a semiconductor cooling device.

[0079] In one embodiment, the temperature control assembly 102 can further include a first temperature sensor and a second temperature sensor (not shown), the first temperature sensor and the second temperature sensor are respectively used to determine the temperature at the first end and the second end as a temperature feedback signal to be fed back to the control circuit 201. Based on the temperature feedback signal, the control circuit 201 can determine whether the real-time temperature at the first end and the second end has reached a suitable temperature respectively, and can further adjust the output power of the first heater 1021 and the second heater 1022 and / or the first cooler and the second cooler, for example, increase the output power, reduce the output power or keep the output power, etc.

[0080] In one embodiment, each reaction vessel 301 can correspond to a plurality of temperature control assemblies 102.

[0081] For example, each reaction vessel 301 can correspond to two temperature control assemblies 102. Two heaters can be arranged at the first end and two heaters can be arranged at the second end. The control circuit 201 can control the output power of the four heaters respectively to control the temperature at each heating point.

[0082] For another example, each reaction vessel 301 can correspond to two temperature control assemblies 102. One heater can be arranged at the first end, one heater can be arranged at the second end, and two heaters can be arranged between the first end and the second end. The control circuit 201 can control the output power of the four heaters respectively to form different temperature gradients between the heating points.

[0083] In addition, in the above two examples, one cooler and one temperature sensor can be further arranged near each heater to cool down the detection module 100 when it is overheated, and to achieve the negative feedback temperature regulation effect.

[0084] It can be understood that the above examples are only for illustrative purposes, and those skilled in the art can reasonably select the number of temperature control assemblies in the detection module according to actual needs, and arrange them at the desired positions, which are not limited herein.

[0085] Continuing as Figure 2AAs shown, in one embodiment, the light source assembly 103 can include a light emitter 1031, which can be used to emit excitation light and irradiate the reaction solution inside the reaction container 301. For example, the light emitter 1031 can be a laser light source, an LED light source, or other light source.

[0086] The emission time of the light emitter 1031 and one or more of the light intensity and the light wavelength of the excitation light can be controlled based on the detection control signal as described above. The emission time of the light emitter 1031 and the light intensity and the light wavelength of the excitation light can be different for different reaction solutions, so that different detection reactions can be achieved.

[0087] In one embodiment, the light sensing assembly 104 can include a light receiver 1041, which can be used to detect the fluorescent signal generated by the reaction solution. For example, the light receiver 1041 can be a PD sensor or other photosensitive sensor, which can convert the detected fluorescent signal into an electrical signal.

[0088] In one embodiment, the light source assembly 103 can further include an excitation light filter 1032 and an excitation light guide medium 1033, and the light sensing assembly 104 can further include a fluorescent light filter 1042 and a fluorescent light guide medium 1043.

[0089] The light guide medium 1033, 1034 can reduce the loss of light during transmission. In one embodiment, the light guide medium 1033, 1034 is a solid transparent material, including silicon-based materials such as glass and quartz, and optical plastics such as polymethyl methacrylate (PMMA), polystyrene (PS), polycarbonate (PC), propylene glycol carbonate (CR39), styrene propylene copolymer (SAN), styrene acrylate copolymer (NAS), poly 4-methyl pentene 1 (TPX), etc. In another embodiment, the ratio of the length to the diameter or the longest diagonal of the light guide medium 1033, 1034 is not greater than 50. In yet another embodiment, the excitation light guide medium 1033 has two ends, one end near the light emitter 1031, which can be referred to as a first distance from the light emitter 1031, and the other end near the reaction container 301, which can be referred to as a second distance from the surface of the reaction container 301, the average distance of the first distance and the second distance being less than 5 millimeters; similarly, the fluorescent light guide medium 1043 has two ends, one end near the light receiver 1041, which can be referred to as a third distance from the light receiver 1041, and the other end near the reaction container 301, which can be referred to as a fourth distance from the surface of the reaction container 301, the average distance of the third distance and the fourth distance being less than 5 millimeters.

[0090] As shown in Table 1 below, according to different fluorescent dye bands, the excitation light filter 1032 can select excitation light of a specific wavelength to irradiate the reaction solution through the excitation light photoconductor 1033, and after the fluorescent signal passes through the fluorescent photoconductor 1043, the fluorescent filter 1042 can select a fluorescent signal of a specific wavelength to be detected by the light receiver 1041.

[0091] Fluorochrome waveband Excitation light filter wavelength (nm) Fluorescence light filter wavelength (nm) FAM 470 520 HEX 530 570 ROX 580 620 CY5 630 675

[0092] Table 1 Filter wavelength settings

[0093] In one embodiment, each reaction vessel 301 may correspond to a plurality of optical assemblies 103, 104, and some or all of the plurality of optical assemblies 103, 104 are symmetrically arranged on the same plane around the reaction vessel 301. As an example, the number of the plurality of optical assemblies 103, 104 may be 2 to 10, and as will be described below, the number of the plurality of optical assemblies 103, 104 may be 4.

[0094] Multiple light source components 103 and multiple photosensitive components 104 can be paired in pairs, the wavelength of the excitation light filter 1032 of each light source component 103 can be the same or different, and the wavelength of the fluorescence filter 1042 of each photosensitive component 104 can be the same or different.

[0095] Multiple light source components 103 and multiple photosensitive components 104 can be paired in pairs according to the characteristics of the fluorescent dye to be detected. When the fluorescent signal of the fluorescent dye is not quenched, different light source component-photosensitive component pairs can detect the fluorescent signals of different fluorescent dyes. Multiple light source component-photosensitive component pairs can simultaneously detect multiple fluorescent signals of multiple fluorescent dyes.

[0096] like Figure 2B As shown, and reference Figure 2A Each reaction container 301 may correspond to four light source components 103a-d and four photosensitive components 104a-d, and the four light source components 103a-d and the four photosensitive components 104a-d may be symmetrically arranged around the reaction container 301 on the same plane.

[0097] The four light source assemblies 103a-d and the four light sensing assemblies 104a-d can be paired two by two, the excitation light filter 1032 of each light source assembly 103a-d can be different in wavelength, and the fluorescence filter 1042 of each light sensing assembly 104a-d can be different in wavelength, so that the fluorescence signals of different wavelengths at the same position can be detected, thereby realizing four-channel detection. Each detection channel can display the detection state through the color of the indicator light, for example, red indicates positive, green indicates negative, and yellow indicates uncertainty, etc. Each detection channel can also display the detection state through the built-in display or the display connected by wire or wireless.

[0098] Figure 3 is another functional assembly of the detection module according to an embodiment of the present application.

[0099] As shown in Figure 3 , each reaction container 301 can correspond to four light source assemblies 103a-d and four light sensing assemblies 104a-d. Unlike Figure 2A and Figure 2B , the four light source assemblies 103a-d and the four light sensing assemblies 104a-d in Figure 3 may not be arranged on the same plane.

[0100] Specifically, the four light sensing assemblies 104a-d can be symmetrically arranged around the reaction container 301 on the same plane, and the four light source assemblies 103a-d can be arranged at one end of the reaction container 301, such as the upper end or the lower end. It can be understood that the four light source assemblies 103a-d can also be symmetrically arranged around the reaction container 301 on the same plane, and the four light sensing assemblies 104a-d can be arranged at one end of the reaction container 301, such as the upper end or the lower end. When the four light source assemblies 103a-d or the four light sensing assemblies 104a-d are arranged at one end of the reaction container 301, the light guide medium 1033, 1034 of the four light source assemblies 103a-d or the four light sensing assemblies 104a-d forms a pyramid or a conical structure, that is, the four light emitters 1031 or the four light receivers 1041 respectively converge to one end of the reaction container 301 along an inner inclination angle (less than 90 degrees but greater than 45 degrees) through the light guide medium 1033, 1034. As shown in Figure 3 , the four light emitters 1031 respectively converge to the lower end of the reaction container 301 along an inner inclination angle (less than 90 degrees but greater than 45 degrees) through the light guide medium 1033, wherein the reaction container 301 is vertically placed, and the included angle between the light guide medium 1033 and the horizontal direction is the inner inclination angle.

[0101] The four light source assemblies 103a-d and the four light sensing assemblies 104a-d can be paired two by two, the excitation light filter 1032 of each light source assembly 103a-d can have different wavelengths, and the fluorescence light filter 1042 of each light sensing assembly 104a-d can have different wavelengths, so that fluorescence signals of different wavelengths can be detected, thereby realizing four-channel detection. Each detection channel can display the detection state through the color of the indicator light, for example, red indicates positive, green indicates negative, and yellow indicates uncertainty, etc. Each detection channel can also display the detection state through the built-in display or the display connected by wire or wireless.

[0102] It can be understood that the above examples are only for illustrative purposes, and a person skilled in the art can reasonably select the number of light source assemblies and light sensing assemblies in the detection module according to actual needs, and set them at desired positions, which are not limited herein.

[0103] Returning to Figure 1 In an embodiment, the detection module 100 can further include a signal processing circuit 105. Similar to the temperature control assembly 102 and the optical assembly 103, 104, for each reaction container 301, the detection module 100 can be provided with an independent signal processing circuit 105. However, it can be understood that the detection module 100 can also provide one signal processing circuit 105 for all reaction containers 301, in other words, in the case that the detection module 100 is configured with multiple reaction containers 301, these reaction containers 301 can also share the signal processing circuit 105.

[0104] The signal processing circuit 105 can be connected with the light sensing assembly 104, and the electrical signal converted by the light sensing assembly 104 is amplified as a detection feedback signal to be fed back to the control circuit 201. The role of amplifying the electrical signal is to amplify the electrical signal to a level that can be recognized by the control circuit 201. It can be understood that the electrical signal converted by the light sensing assembly 104 can be an analog signal or a digital signal, and when the electrical signal is an analog signal, the signal processing circuit 105 further converts the electrical signal into an analog-digital signal. The signal characteristics of the processed electrical signal are associated with the signal characteristics of the fluorescence signal.

[0105] Continuing as shown in Figure 1 In an embodiment, the detection module 100 can further include a communication interface 106. The communication interface 106 can be connected with the temperature control assembly 102 to send a temperature control signal and receive a temperature feedback signal, the communication interface 106 can be connected with the light source assembly 103 to send a detection control signal, and the communication interface 106 can also be connected with the signal processing circuit 105 to receive a detection feedback signal.

[0106] The master module 200 can further comprise a communication circuit 202, which can be connected with the control circuit 201. The detection module 100 and the master module 200 can communicate via the communication interface 106 and the communication circuit 202, so that the master module 200 can send temperature control signals and detection control signals to the detection module 100, and can receive temperature feedback signals and detection feedback signals from the detection module 100.

[0107] It can be understood that the communication mode between the detection module 100 and the master module 200 can adopt wired connection (such as cable, USB connection, etc.) and / or wireless connection (such as WiFi, Bluetooth, 5G / 6G connection, etc.), which is not limited herein.

[0108] Continuing as shown in Figure 1 In one embodiment, the master module 200 can further comprise a display circuit 203, which can be connected with the control circuit 201, so as to receive the detection results generated by the control circuit 201 based on the detection feedback signals, and show the detection results to the user.

[0109] It can be understood that the master module 200 can be connected with a host computer (not shown) such as a personal computer, and the communication mode can adopt wired connection (such as cable, USB connection, etc.) and / or wireless connection (such as WiFi, Bluetooth, 5G / 6G connection, etc.). The software operation program or data analysis program of the master module 200 can be written and modified through the host computer. The data analysis of the detection results can be completed in the master module 200 or the host computer.

[0110] Figures 4A to 4C respectively are the front view, the elevation view and the top view of the installation structure of the detection module according to one embodiment of the present application. As an example, the size of one detection module is about 20cm (length) x 30cm (width) x 50cm (height), and the size of the whole detection device (including the master module, the rechargeable battery, the shell and one detection module) can be less than 70cm x 70cm x 70cm.

[0111] As shown in Figures 4A to 4C The detection module 100 can be provided with multiple installation plates such as printed circuit boards (PCB) in the length direction of the reaction container 301, and can be provided with functional components such as the first heater 1021 and the second heater 1022, and the light source assembly 103, the photosensitive assembly 104 and the communication interface 106, etc. on the corresponding installation plates or between two installation plates.

[0112] The detection module in the present invention has an independent and complete temperature control and optical structure design, that is, it has a dedicated plurality of temperature control components, a plurality of optical components (including a light source component and a photosensitive component), a signal processing circuit and a communication interface. The detection module can be controlled by a main control module, and can simultaneously detect one or more types of fluorescent signals generated by a single or multiple nucleic acid amplification reaction, thereby realizing modularization, combination, miniaturization and structural standardization of the detection device, with high scalability and low production cost. In addition, compared to existing detection devices, the detection device of the present invention can obtain multiple detection results more quickly in a smaller volume in a variety of application scenarios (such as bedside detection and point-of-care testing (POCT)).

[0113] Figure 5 This is a front view of the installation structure of the light source assembly and the photosensitive assembly according to an embodiment of the present invention.

[0114] like Figure 5 As shown, the detection module may include a first bracket 107 and a second bracket 108. The first bracket 107 may be a polygonal block having a central through hole 1071, and the second bracket 108 may be a rectangular block having a recess 1081. The recess 1081 of the second bracket 108 may have a central through hole 1082, and the shape of the recess 1081 may be adapted to the shape of the first bracket 107, so that the first bracket 107 can be configured to the recess 1081. In this case, the central through hole 1071 of the first bracket 107 and the central through hole 1082 of the recess 1081 can jointly serve as a cavity for configuring a reaction vessel.

[0115] Through holes 1072 can also be provided on multiple side surfaces of the first bracket 107. These through holes 1072 can be used to accommodate optical filters and light guides. Multiple mounting slots 1083 can be provided around the recess 1081. These mounting slots 1083 can be used to accommodate optical emitters or optical receivers. After the first bracket 107 is positioned within the recess 1081, the optical emitter emits excitation light. This excitation light passes through the optical filters and light guides within one through hole 1072 and illuminates the reaction solution within the reaction vessel positioned within the cavity. The fluorescent signal generated by the reaction solution passes through the optical filters and light guides within the other through hole 1072 and is detected by the light receiver.

[0116] Figure 6is a temperature feedback signal graph of a single-module multiplex detection device according to an embodiment of the present application. In this embodiment, the single detection module includes one temperature control component, i.e., a first heater located at the upper end of the reaction vessel and a second heater located at the lower end of the reaction vessel. The upper end temperature and the lower end temperature of the reaction vessel vary with time under the control of the master module, and are achieved through the temperature control component. In this embodiment, the pressure inside the reaction vessel can be maintained above one atmosphere, so that the lower end temperature can be set to be higher than 100°C, such as 100°C to 105°C. Under this condition, the DNA polymerase used still has activity to amplify nucleic acids.

[0117] Figure 7 is a detection feedback signal graph of a single-module multiplex detection device according to an embodiment of the present application. In this embodiment, the single detection module includes four optical components, i.e., four light source components and four light receiving components. There are three sets of amplification primers and three corresponding detection probes in the reaction system, which correspond to three DNA nucleic acid target molecules, each with 10 copies. From the four detection feedback signal curves, it can be seen that, in addition to the negative control (#4), the three low-copy DNA nucleic acid target molecules can be detected simultaneously within 20 minutes (1200 seconds) (#1, #2 and #3), which illustrates the high sensitivity, rapidity, miniaturization and multiplex detection features of the present application.

[0118] Second embodiment

[0119] A second embodiment of the present application provides a multi-module multiplex detection device.

[0120] Figure 8 is a partial schematic diagram of a multi-module multiplex detection device according to an embodiment of the present application.

[0121] As Figure 8 shown, the multi-module multiplex detection device can include a plurality of detection modules 100, 100a-n and a master module (not shown), wherein each detection module 100, 100a-n belongs to the same inventive concept as the detection module in the first embodiment, and the master module also belongs to the same inventive concept as the master module in the first embodiment. The technical details mentioned in the first embodiment are still valid in this embodiment, and in order to reduce repetition, they will not be repeated here.

[0122] It can be understood that, Figure 8Four detection modules are shown for illustrative purposes, and the number of detection modules can be reasonably selected by those skilled in the art according to actual needs, which is not limited herein. As an example, the number of detection modules can be 2 to 10 or 2 to 100, so that one master control module can control multiple detection modules, such as 2 to 10 or 2 to 100 detection modules, that is, one detection device can simultaneously detect one or more types of fluorescent signals of 2 to 10 or 2 to 100 samples.

[0123] Figure 9 is a communication diagram of a multi-module multiplex detection device according to an embodiment of the present application.

[0124] As shown in Figure 9 , and referring to Figure 8 , the control circuit 201 in the master control module can communicate with the communication interfaces (not shown) of the plurality of detection modules 100, 100a-n via the communication circuit 202, respectively, to send control signals to each detection module 100, 100a-n and receive feedback signals from each detection module 100, 100a-n.

[0125] Since the detection modules in the present application are independent structures with dedicated temperature control components, multiple optical components (including light source components and photosensitive components), signal processing circuits and communication interfaces, the detection modules can be freely combined, and each detection module can share a master control module and be independently controlled by the master control module without being affected by other detection modules, so that instruments with multiple amplification and multiple detection capabilities of different detection fluxes can be customized according to needs and application scenarios, the instrument development cycle is short, and the production cost is low.

[0126] It can be understood that in the multi-module multiplex detection device, each detection module can also be individually controlled by a sub-control module, and the structure of the sub-control module can be the same as or similar to the master control module described above.

[0127] Third embodiment

[0128] The third embodiment of the present application provides a detection method of a modular multiplex detection device.

[0129] The detection method of the modular multiplex detection device can include the following steps:

[0130] The reaction vessel injected with the reaction solution can be arranged in the cavity of the corresponding detection module, wherein the reaction solution can include a reaction system and a detection sample, and the detection sample can have one or more target molecules to be detected;

[0131] A temperature control signal can be sent by the control circuit of the master module to each detection module (hereinafter referred to as each detection module) configured with a reaction container, to control one or more temperature control components of each detection module to conduct heat to the corresponding reaction container and the reaction solution inside it, so that the reaction solution can form a thermal convection;

[0132] A detection control signal can be sent by the control circuit to each detection module to control the light source component in one or more optical components of each detection module to emit excitation light and irradiate the reaction solution inside the corresponding reaction container;

[0133] One or more types of fluorescent signals generated by the reaction solution inside the corresponding reaction container can be detected by the photosensitive component in one or more optical components of each detection module, so that the signal processing circuit of each detection module can generate one or more detection feedback signals;

[0134] The control circuit can receive one or more detection feedback signals from each detection module.

[0135] Under the action of the plurality of temperature control components, one or more free fluorescent dyes specific to one or more target molecules and associated with the amount of one or more target molecules can be generated in the nucleic acid amplification reaction in the reaction container, and under the action of the plurality of optical components, one or more types of fluorescent signals generated by the excitation of one or more free fluorescent dyes are not quenched and detected, so that multiple types of fluorescent signals can be detected simultaneously from one reaction container to achieve multiplex detection.

[0136] Each detection module belongs to the same inventive concept as the detection module in the first and second embodiments, and the master module also belongs to the same inventive concept as the master module in the first and second embodiments. The technical details mentioned in the first and second embodiments are still valid in this embodiment. In order to reduce repetition, they will not be repeated here.

[0137] In one embodiment, the detection method of the modular multiplex detection device can further include the following steps:

[0138] The temperature at a plurality of heating points of the corresponding reaction container can be determined by one or more temperature control components of each detection module, so that each detection module can generate a plurality of temperature feedback signals;

[0139] The control circuit can receive a plurality of temperature feedback signals from each detection module.

[0140] In one embodiment, the target molecule can include one or more of an RNA molecule or a DNA molecule, an RNA fragment in an RNA genome or a DNA fragment in a DNA genome, and a variant structure in an RNA molecule or a DNA molecule.

[0141] In one embodiment, the variant structure can include Single Nucleotide Polymorphisms (SNP).

[0142] In one embodiment, the target molecule can include one or more of RNA viral nucleic acid molecules and DNA viral nucleic acid molecules. In other words, the target molecule can include only RNA viral nucleic acid molecules, can include only DNA viral nucleic acid molecules, or can include both RNA viral nucleic acid molecules and DNA viral nucleic acid molecules.

[0143] The RNA virus can include one or more of 14 common respiratory RNA viruses, including Influenza A virus InfA, Influenza A virus H1N1 2009, Influenza A virus H3N2, Human Parainfluenza virus HPIV1, Human Parainfluenza virus HPIV2, Human Parainfluenza virus HPIV3, Human Parainfluenza virus HPIV4, Human Metapneumovirus hMPV, Respiratory Adenovirus AdV, Respiratory Syncytial Virus RSV, Bocavirus BoV, Severe Acute Respiratory Syndrome Coronavirus SARS-CoV, Middle East Respiratory Syndrome Coronavirus MERS-CoV, and Severe Acute Respiratory Syndrome Coronavirus 2 SARS-CoV-2.

[0144] The DNA virus can include one or more of 8 common human herpes DNA viruses, including Human Herpes Virus HSV-1, Human Herpes Virus HSV-2, Human Herpes Virus VZV, Human Herpes Virus CMV, Human Herpes Virus EBV, Human Herpes Virus HHV-6, Human Herpes Virus HHV-7, and Human Herpes Virus HHV-8.

[0145] In one embodiment, the target molecule can be derived from human, animal, plant, microorganism, or artificially or chemically synthesized, wherein the microorganism can include one or more of virus, bacteria, and fungus.

[0146] In this article, the reaction system refers to the liquid injected into the inside of the reaction space provided by the reaction container for mixing with the detection sample, in other words, refers to all the liquid in the reaction space inside except the detection sample in the detection process of the modular multiplex detection device. The reaction system can have at least two functions, one is to provide a liquid enzymatic reaction environment for multiplex amplification, and the other is to provide conditions for molecular hybridization of target molecules with nucleic acid primers and nucleic acid probes (also known as detection probes).

[0147] In one embodiment, the reaction system can include multiple pairs of primers corresponding to multiple types of target molecules respectively, which can be used to hybridize with the corresponding target molecules in the detection sample to achieve amplification, so as to increase the concentration of the target molecules through molecular amplification under the action of thermal convection, and increase the hybridization opportunity of the molecules of the nucleic acid probe with the target molecules. The molecular amplification can include polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), nicking endonuclease isothermal amplification technology (NEAR), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA), helicase-dependent amplification (HDA), recombinase polymerase amplification (RPA), and enzyme recombination isothermal amplification technology (ERA).

[0148] As shown in Table 2 below, for different types of target molecules, corresponding primers for one or more nucleic acid amplification reactions can be prepared to be added to the reaction system, and corresponding nucleic acid probes for detecting one or more target molecules can be prepared.

[0149] In one embodiment, the quencher and the fluorescent dye (not shown) can be respectively arranged at the two ends of the nucleic acid probe; during the nucleic acid amplification process, the quencher and the fluorescent dye are separated under the action of the exonuclease or the endonuclease, so that the fluorescence signal generated by the free fluorescent dye after excitation is not quenched by the quencher, and thus is detected.

[0150]

[0151]

[0152] Table 2 Target molecule sequences and corresponding primer sequences and nucleic acid probe sequences

[0153] In one embodiment, the reaction system can include primers, nucleic acid probes, and DNA polymerase. In another embodiment, the reaction system can include MgCl2 with a concentration of 3 mM, dNTP with a concentration of 0.2 mM, multiple primers with concentrations of 0.1 μM to 0.6 μM respectively, DNA polymerase with a concentration of 0.05 U / μl, RNA reverse transcriptase with a concentration of 0.5 U / μl, DTT with a concentration of 1 mM, Tween-20 with a concentration of 0.05%, Tris-HCl with a pH of 8.8 and a concentration of 25 mM, and K2SO4 with a concentration of 30 mM. In yet another embodiment, the DNA polymerase has 3'→5' exonuclease activity. By using a polymerase with 3'→5' exonuclease activity, the detection rate for mutated virus strains can be increased, the paired bases of the primers can be repaired, and high fidelity can be achieved, reducing invalid amplification products, so that the detection result is more reliable.

[0154] In one embodiment, the volume of the reaction system can be 50 μl.

[0155] It can be understood that the multiple types of target molecules listed above can be used only for illustration, but not for limitation. In fact, other types of target molecules can be set by those skilled in the art according to actual needs, and the corresponding primer sequences and nucleic acid probe sequences thereof can be prepared, and the reaction system can be adjusted to achieve the effect of multiplex amplification and multiplex detection of multiple types of target molecules in the same liquid-phase reaction system.

[0156] While the application has been illustrated and described in detail in the drawings and foregoing description, the ordinary skilled person will be aware that various changes in form and detail can be made without departing from the spirit and scope of the application.

Claims

1. A modular multiple detection device, characterized in that: The device comprises: One or more detection modules, each detection module comprising a cavity for arranging a reaction vessel, a plurality of temperature control components and a plurality of optical components corresponding to the reaction vessel, and a signal processing circuit, wherein the reaction vessel is a container for nucleic acid amplification reaction; A main control module, the main control module including a control circuit, the control circuit being configured to send a control signal to each detection module and receive a feedback signal from each detection module; Each optical assembly includes a light source assembly and a photosensitive assembly, wherein the light source assembly includes a light emitter, the light emitter is used to emit excitation light and irradiate the reaction solution inside the reaction container, and the photosensitive assembly includes a light receiver, the light receiver is used to detect a fluorescent signal generated by the reaction solution and convert the detected fluorescent signal into an electrical signal; The light source assembly further comprises an excitation light filter and an excitation light guide medium, and the photosensitive assembly further comprises a fluorescence filter and a fluorescence light guide medium, and the average distance between one end of the light guide medium and the light emitter or the light receiver and the other end of the light guide medium and the surface of the reaction container is less than 5 mm; Wherein, the multiple light source assemblies and the multiple photosensitive assemblies are not arranged on the same plane, wherein the multiple photosensitive assemblies are symmetrically arranged around the reaction vessel on the same plane and the multiple light source assemblies are arranged at one end of the reaction vessel, or wherein the multiple light source assemblies are symmetrically arranged around the reaction vessel on the same plane and the multiple photosensitive assemblies are arranged at one end of the reaction vessel, when the multiple light source assemblies or the multiple photosensitive assemblies are arranged at one end of the reaction vessel, the photoconductive media of the multiple light source assemblies or the multiple photosensitive assemblies form a pyramid or cone-shaped structure, and the light emitters of the multiple light source assemblies or the light receivers of the multiple photosensitive assemblies are respectively converged to one end of the reaction vessel along an inner inclination angle of less than 90 degrees but greater than 45 degrees through the photoconductive medium, wherein the reaction vessel is placed vertically, and the angle formed by the photoconductive medium and the horizontal direction is the inner inclination angle; Wherein, each detection module includes a first bracket and a second bracket, the first bracket is a polygonal block with a central through hole, and the second bracket is a rectangular block with a recess, the recess of the second bracket has a central through hole and the shape of the recess is adapted to the shape of the first bracket, the first bracket is configured to the recess, and the central through hole of the first bracket and the central through hole of the recess together serve as the cavity for configuring the reaction container; through holes are respectively arranged on multiple side surfaces of the first bracket, when the multiple photosensitive components or the multiple light source components are symmetrically arranged around the reaction container on the same plane, the through holes are used to configure the filters and the light-conducting media of the multiple photosensitive components or the multiple light source components; multiple mounting grooves are arranged around the recess, and the multiple mounting grooves are used to respectively configure the light receivers of the multiple photosensitive components or the light emitters of the multiple light source components.

2. The device according to claim 1, characterized in that Each temperature control assembly includes a first heater and a second heater, the first heater being located at a first end of the reaction vessel, and the second heater being located at a second end of the reaction vessel opposite the first end.

3. The device according to claim 2, characterized in that The control signal includes a temperature control signal, and output powers of the first heater and the second heater are controlled based on the temperature control signal to transfer heat to the reaction container and the reaction solution therein.

4. The device according to claim 3, characterized in that Each temperature control assembly further includes a first temperature sensor and a second temperature sensor, wherein the first temperature sensor and the second temperature sensor are used to respectively determine the temperature at the first end and the second end to be fed back to the control circuit as a temperature feedback signal.

5. The device according to claim 1, characterized in that The control signal includes a detection control signal, and an emission time of the light emitter and one or more of a light intensity and a light wavelength of the excitation light are controlled based on the detection control signal.

6. The device according to claim 1, characterized in that The light guiding medium is a solid transparent material, and the ratio of the length to the diameter or the longest diagonal of the light guiding medium is not greater than 50.

7. The device according to claim 1, characterized in that The multiple light source components and the multiple photosensitive components are paired in pairs, the wavelength of the excitation light filter of each light source component is the same or different, and the wavelength of the fluorescence filter of each photosensitive component is the same or different.

8. The device according to claim 1, characterized in that The number of the plurality of optical components is 2 to 10.

9. The device according to claim 8, characterized in that The number of the plurality of optical components is four.

10. The device according to claim 1, characterized in that The signal processing circuit is used to amplify and perform analog-to-digital conversion on the electrical signal converted by the optical receiver so as to feed the electrical signal back to the control circuit as a detection feedback signal.

11. The device according to claim 1, characterized in that Each detection module further includes a communication interface, and the main control module further includes a communication circuit. Each detection module and the main control module communicate with each other via the communication interface and the communication circuit.

12. The device according to claim 1, characterized in that The main control module also includes a display circuit, which is used to display the detection results of each detection module.

13. The device according to claim 1, characterized in that The number of the one or more detection modules is 1, 2 to 10, or 2 to 100.

14. A detection method for a modular multiplex detection device according to any one of claims 1 to 13, wherein the method is used for purposes other than disease treatment or diagnosis, characterized in that: The method comprises: Arrange a reaction container filled with a reaction solution into a cavity of a corresponding detection module, wherein the reaction solution includes a reaction system and a detection sample, and the detection sample contains one or more target molecules to be detected; The control circuit of the main control module sends a temperature control signal to each detection module configured with the reaction container, so as to control the multiple temperature control components of each detection module to transfer heat to the corresponding reaction container and the reaction solution therein, so that the reaction solution can form heat convection; The control circuit sends a detection control signal to each detection module to control the light source assembly in the multiple optical assemblies of each detection module to emit excitation light and irradiate the reaction solution inside the corresponding reaction container; Detecting one or more types of fluorescent signals generated by the reaction solution in the corresponding reaction container by a photosensitive component among the multiple optical components of each detection module, so that a signal processing circuit of each detection module can generate one or more detection feedback signals; The one or more detection feedback signals are received by the control circuit from each detection module.

15. The method according to claim 14, characterized in that The method further comprises: The multiple temperature control components of each detection module determine the temperature at multiple heating points of the corresponding reaction container, so that each detection module can generate multiple temperature feedback signals; The control circuit receives the plurality of temperature feedback signals from each detection module.

16. The method according to claim 14, characterized in that The target molecule includes one or more of an RNA molecule or a DNA molecule, an RNA fragment in an RNA genome or a DNA fragment in a DNA genome, and a variant structure in an RNA molecule or a DNA molecule.

17. The method according to claim 16, characterized in that The target molecule originates from humans, animals, plants, microorganisms, or is artificially or chemically synthesized, wherein the microorganisms include one or more of viruses, bacteria, and fungi.

18. The method according to claim 14, characterized in that The reaction system includes primers for single or multiple nucleic acid amplification reactions, one or more detection probes for detecting the one or more target molecules, and DNA polymerase, wherein each detection probe is equipped with a fluorescent dye corresponding to the corresponding target molecule.

19. The method according to claim 18, characterized in that The DNA polymerase has 3'→5' exonuclease activity.

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