Planting method for preventing and controlling citrus Huanglongbing and ulcer disease

Abstract

This invention discloses a planting method for preventing and controlling Huanglongbing and Citrus Canker in citrus, belonging to the field of citrus control technology. This application includes the following steps: Step 1: Spread 10-20 kg of fungicide and 8-20 kg of insecticide per mu of planting land, then deep plow to a depth of 15-30 cm, break up the turned-up soil, irrigate, cover with a transparent film and let it stand for 8-16 days. After standing, remove the film, plow, dry, and ridge; Step 2: Dig planting pits on the ridges and plant citrus seedlings in the planting pits; Step 3: Weed the ridges; Step 4: During the spring, summer, autumn, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 10-15 days. If it enters the recurrence period, spray the aforementioned insecticide every 7-10 days; Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the aforementioned fungicide every 15-20 days. During the summer, autumn, and post-flowering periods of citrus trees, spray the citrus trees with the aforementioned fungicide every 10-15 days. This invention effectively reduces the incidence of Huanglongbing and citrus canker in citrus.

Description

Technical Field

[0001] This invention belongs to the field of citrus disease control technology, specifically relating to a planting method for controlling citrus Huanglongbing and citrus canker. Background Technology

[0002] Citrus fruits, including mandarins, oranges, pomelos, lemons, and tangerines, are diverse and abundant, making them a major fruit category in my country and widely loved. With advancements in technology, the citrus industry has developed rapidly, with planting areas continuously expanding. Consequently, the requirements for citrus cultivation and management have become increasingly stringent. Inadequate cultivation management and disease control can easily lead to citrus diseases such as Huanglongbing (HLB) and citrus canker.

[0003] Citrus Huanglongbing (HLB) is caused by *Bacillus thuringiensis*, a highly contagious bacterium. It is primarily transmitted by the citrus psyllid. After biting young citrus shoots, the psyllid injects the HLB pathogen from its mouth and digestive tract into the citrus tree. The pathogen is then transported downwards through the phloem to the roots, causing root rot and a sharp decline in absorption capacity, severely impacting tree growth. Infected trees also fail to produce normal fruit, thus affecting economic returns. Currently, the control of HLB primarily focuses on "disease-free seedlings, psyllid control, and pruning diseased trees." Disease-free seedlings refer to planting citrus seedlings free of pathogens, while pruning diseased trees involves cutting down and removing infected plants. Psyllid control aims to manage the psyllid population in orchards and prevent outbreaks of Huanglongbing (HLB). Chemical insecticides (such as imidacloprid and cypermethrin) are generally used for control. However, psyllids are highly mobile and easily fly away when sprayed, making it difficult for the pesticide to reach them and achieve good insecticidal effects. This makes control and management inconvenient, and long-term, large-scale use also increases the psyllids' resistance to pesticides.

[0004] Citrus canker is a major bacterial disease affecting citrus trees, causing cankers on the twigs, extensive leaf infection, and fruit deformation or even rotting, rendering the fruit inedible. This disease is highly contagious and difficult to control, making it a persistent challenge for citrus plants. Currently, the most commonly used methods for controlling citrus canker are fungicides, primarily copper-based single agents (such as copper hydroxide, copper oxide, and copper hydroxide) and copper-based compound agents (such as kasugamycin-copper hydroxide, copper hydroxide-zinc hydroxide, kasugamycin-quinoline copper, and copper acetate). However, copper-based agents have significant limitations. They promote oviposition and larval growth in female mites, and excessive use can easily stimulate spider mite infestations. Conversely, low concentrations are insufficient to kill the pathogens and control canker. Spider mites, on the other hand, can reproduce sexually and parthenogenetically, exhibiting high reproductive capacity and rapid reproduction rates, making them equally difficult to control. In response, while using copper-based agents to control citrus canker, insecticidal chemicals are also added to kill spider mites, but these also kill predatory mites, which is not conducive to biological control. At the same time, the large-scale use of chemical agents can easily pollute the environment and result in high costs for prevention, control, planting and management.

[0005] Therefore, there is a need to provide a planting method that is effective in preventing and controlling citrus Huanglongbing and Citrus Canker, while also having low management costs. Summary of the Invention

[0006] This invention provides a planting method for preventing and controlling Huanglongbing and Citrus Canker, in order to solve the technical problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A planting method for preventing and controlling Huanglongbing and Citrus Canker includes the following steps:

[0009] Step 1: Soil management. Spread 10-20 kg of fungicide and 8-20 kg of insecticide per acre of planting land, then plow to a depth of 15-30 cm, break up the soil that has been turned up, then irrigate, cover with a transparent film and let it stand for 8-16 days. After the standing period, remove the film, plow, dry, and ridge.

[0010] Step 2: Planting. Dig planting pits on the ridges and plant citrus seedlings in the pits.

[0011] Step 3: Weed the ridges;

[0012] Step 4: During the spring shoots, summer shoots, autumn shoots, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 10-15 days. If the disease enters a severe outbreak period, spray the aforementioned insecticide every 7-10 days.

[0013] Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the fungicide every 15-20 days. During the summer shoot, autumn shoot, and post-flowering period of citrus trees, spray the citrus trees with the fungicide every 10-15 days.

[0014] Step Six: Clean the orchard regularly, pruning diseased, overgrown, and weak branches, and regularly removing fallen leaves, fallen fruit, and dead branches.

[0015] As a further improvement to the technical solution, the active ingredients of the bactericide include zinc thiazolyl and orange peel essential oil.

[0016] As a further improvement to the technical solution, the bactericide, by mass percentage, comprises the following components: 10-25% zinc thiazole, 10-21% orange peel essential oil, 2-8% dispersant, 3-7% wetting agent, 0.3-2% thickener, 1-5% antifreeze, 0.1-0.8% preservative, 0.2-1.2% defoamer, with the balance being water; wherein the dispersant includes at least one of lignin sulfur salt, polycarboxylate, and polyoxyethylene ether phosphate; the wetting agent includes at least one of resin polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, and sodium lauryl ether sulfate; the thickener includes one or more of xanthan gum, carboxymethyl cellulose, magnesium aluminum silicate, and polyvinyl alcohol; the antifreeze includes one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol; the preservative includes one or more of paraformaldehyde, sodium benzoate, and potassium sorbate; and the defoamer includes one or more of silicone oil, organosilicone, and polysiloxane.

[0017] As a further improvement to the technical solution, the bactericide, by mass percentage, comprises 18% zinc thiazole, 17% orange peel essential oil, 4% dispersant, 5% wetting agent, 1.1% thickener, 3% antifreeze, 0.4% preservative, 0.7% defoamer, and the remainder is water.

[0018] As a further improvement to the technical solution, the active ingredients of the insecticide include pyriproxyfen, pymetrozine, and azadirachtin.

[0019] As a further improvement to the technical solution, the insecticide, by weight percentage, comprises the following components: 6-18% pyriproxyfen, 3-12% pymetrozine, 5-17% azadirachtin, 3-7% dispersant, 0.1-3% thickener, 1-4% antifreeze, 0.4-2% defoamer, with the balance being water; wherein the dispersant includes at least one of lignin sulfur salt, polycarboxylate, and polyoxyethylene ether phosphate; the wetting agent includes at least one of resin polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, and sodium lauryl ether sulfate; the thickener includes one or more of xanthan gum, carboxymethyl cellulose, magnesium aluminum silicate, and polyvinyl alcohol; the antifreeze includes one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol; and the defoamer includes one or more of silicone oil, organosilicone, and polysiloxane.

[0020] As a further improvement to the technical solution, the insecticide, by mass percentage, comprises 10% pyriproxyfen, 8% pymetrozine, 12% azadirachtin, 5% dispersant, 2% thickener, 3% antifreeze, 1% defoamer, and the remainder is water.

[0021] As a further improvement to the technical solution, before performing step one, the following steps are also included:

[0022] Install planting sheds on the planting land;

[0023] The planting shed includes:

[0024] skeleton;

[0025] A rainproof structure is provided on the top and bottom surfaces of the frame;

[0026] An insect-proof net is installed on the outside of the frame, covering the outside of the frame.

[0027] As a further improvement to the technical solution, the planting shed also includes: a first linear moving device and a spraying device; the first linear moving device is aligned on both sides of the interior of the planting shed; the first linear moving device is vertically arranged; the first linear moving device includes a motor, a guide rail, a lead screw, and a connecting block; the lead screw is rotatably mounted on the guide rail; the motor is located at one end of the guide rail; the output end of the motor is mechanically connected to one end of the lead screw, driving the lead screw to rotate; the connecting block is located on the lead screw; the spraying device includes a housing, a hose, a first pipe, a second pipe, a circular pipe, a connecting plate, and a spray nozzle. The device includes a nozzle and a pump; a connecting plate connected to a connecting block; both ends of the connecting plate facing the guide rail; a first pipe mounted on the connecting plate; one end of a second pipe connected to the first pipe, and the other end facing the ground and connected to the annular pipe; the second pipe is vertically arranged; multiple second pipes are spaced apart along the length of the connecting plate; the annular pipe is horizontally arranged; the nozzle is connected to the annular pipe; multiple nozzles are spaced apart on the inner side of the annular pipe; the pump is mounted on the flexible hose; one end of the flexible hose is connected to the housing, and the other end is connected to one end of the first pipe.

[0028] As a further improvement to the technical solution, the end of the first pipe away from the hose is a closed structure.

[0029] As a further improvement to the technical solution, the axial direction of the annular tube is consistent with the orientation of the first linear moving device.

[0030] As a further improvement to the technical solution, the planting shed also includes a second linear moving device; the second linear moving device is located on both sides inside the planting shed where the guide rail is provided; the second linear moving device has the same structure as the first linear moving device; the connecting block of the second linear moving device is connected to one end of the guide rail of the first linear moving device; the orientation of the guide rail of the second linear moving device is perpendicular to the guide rail of the first linear moving device.

[0031] As a further improvement to the technical solution, the planting shed also includes a slide rail; the slide rail is located on both sides inside the planting shed, on the same side as the second linear moving device; the slide rail is mounted on the frame; the slide rail is located above the second linear moving device; the orientation of the slide rail is the same as the orientation of the second linear moving device; the guide rail of the first linear moving device is slidably connected to the slide rail.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0033] 1. This invention involves spraying citrus trees with a mixture of thiamethoxam zinc and orange peel essential oil. The synergistic effect of these two agents effectively reduces the incidence of citrus canker. Thiamethoxam zinc, a zinc-based formulation, does not attract spider mites, thus simplifying citrus cultivation and management and reducing the need for other pesticides. Orange peel essential oil possesses strong bactericidal properties, its mechanism of action primarily based on dissolving the phospholipid layer of pathogen spores. This dissolution of the phospholipid layer leads to the loss of the protective function of the pathogen's cell membrane, causing the natural loss of cell sap and ultimately resulting in the pathogen's drying and death.

[0034] 2. This invention involves spraying citrus trees with a mixture of pyriproxyfen, pymetrozine, and azadirachtin in a specific ratio. The synergistic effect of these three agents effectively eliminates psyllids, thereby reducing the incidence of citrus Huanglongbing (HLB). Pyriproxyfen is a juvenile hormone analog insect growth regulator that disrupts insect physiological functions, leading to prolonged larval instars or the formation of over-instar larvae, resulting in death. Pymetrozine is systemic, exerting contact killing effects on pests and also having some ovicidal activity. Azadirachtin can directly or indirectly induce feeding refusal by damaging the chemosensory organs of insect mouthparts; it also affects the midgut digestive enzymes, resulting in insufficient nutrient conversion of food and impacting insect vitality.

[0035] 3. The spraying device of the present invention can spray the area around the citrus tree from top to bottom, so that the top and bottom surfaces of the leaves of the citrus tree are sprayed with water or pesticide. When applying pesticides or irrigating, the first linear moving device and the pump are activated. The first linear moving device drives the connecting plate to descend. The first pipe, the second pipe and the circular pipe descend with the connecting plate. The circular pipe moves towards the citrus tree, with one circular pipe corresponding to one citrus tree. The circular pipe passes through the citrus tree, first from the top of the citrus tree to the bottom of the citrus tree, and then returns from the bottom of the citrus tree. When the circular pipe passes through the citrus tree, the pump delivers water or pesticide to the circular pipe. The water or pesticide is sprayed onto the citrus tree through the nozzle. In particular, it can effectively reduce the hiding of psyllids and increase the contact between psyllids and pesticides, especially in the prevention and control of Huanglongbing (HLB). This can further eliminate psyllids. Attached Figure Description

[0036] To more clearly illustrate the technical solutions of the embodiments of the invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0037] Figure 1 Schematic diagram of the structure of the first linear moving device and spraying device provided for the invention Figure 1 ;

[0038] Figure 2 Schematic diagram of the structure of the first linear moving device and spraying device provided for the invention Figure 2 ;

[0039] Figure 3 A schematic diagram of the external structure of the planting shed provided for the invention;

[0040] Figure 4 for Figure 3 Internal structural sectional view;

[0041] Figure 5 for Figure 4 Sectional view at point A in the middle;

[0042] Reference numerals: 1-Frame, 2-Rainproof structure, 3-Insect net, 4-First linear movement device, 41-Motor, 42-Guide rail, 43-Screw, 44-Connecting block, 5-Spraying device, 51-Box, 52-Hose, 53-First pipe, 54-Second pipe, 55-Circular pipe, 56-Connecting plate, 57-Sprayer head, 58-Pump, 6-Second linear movement device, 61-Motor, 62-Guide rail, 63-Screw, 64-Connecting block, 7-Slide rail. Detailed Implementation

[0043] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the described embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise defined, the technical or scientific terms used herein should have the ordinary meaning understood by those skilled in the art to which this invention pertains.

[0044] All the substances in the bactericidal agent described in this invention can be purchased on the market, and the manufacturers of the substances will not be emphasized here.

[0045] Example 1:

[0046] A planting method for preventing and controlling Huanglongbing and Citrus Canker includes the following steps:

[0047] Step 1: Soil management. Spread 10 kg of fungicide and 8 kg of insecticide per acre of planting land, then plow to a depth of 15 cm, break up the soil that has been turned up, then irrigate, cover with a transparent film and let it stand for 8 days. After standing, remove the film, then plow, dry, and ridge.

[0048] Step 2: Planting. Dig planting pits on the ridges and plant citrus seedlings in the pits.

[0049] Step 3: Weed the ridges. We can lay weed control cloth or other materials on the ridges to prevent weeds from growing and to avoid psyllids hiding in the weeds.

[0050] Step 4: During the spring shoots, summer shoots, autumn shoots, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 10 days. If the disease enters a severe outbreak period, spray the aforementioned insecticide every 7 days.

[0051] Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the fungicide every 15 days; during the summer shoot period, autumn shoot period, and after flowering, spray the citrus trees with the fungicide every 10 days.

[0052] Step Six: Clean the orchard regularly, pruning diseased, overgrown, and weak branches, and regularly removing fallen leaves, fallen fruit, and dead branches.

[0053] This embodiment also discloses a bactericide, the effective components of which include thiazolium zinc and orange peel essential oil; the bactericide, by mass percentage, comprises 18% thiazolium zinc, 17% orange peel essential oil, 4% dispersant, 5% wetting agent, 1.1% thickener, 3% antifreeze agent, 0.4% preservative, and 0.7% defoamer, wherein the dispersant includes lignin sulfur salt and polycarboxylate, the wetting agent includes resin polyoxyethylene ether and alkylphenol formaldehyde resin polyoxyethylene ether, the thickener includes xanthan gum and polyvinyl alcohol, the antifreeze agent includes ethylene glycol and polyethylene glycol, the preservative includes paraformaldehyde and potassium sorbate, and the defoamer includes silicone oil.

[0054] This embodiment also discloses an insecticide, the active ingredients of which include pyriproxyfen, pymetrozine, and azadirachtin; the insecticide, by mass percentage, comprises 10% pyriproxyfen, 8% pymetrozine, 12% azadirachtin, 5% dispersant, 2% thickener, 3% antifreeze agent, and 1% defoamer, wherein the dispersant includes lignin sulfur salt, the wetting agent includes resin polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, and sodium lauryl ether sulfate, the thickener includes xanthan gum and carboxymethyl cellulose, the antifreeze agent includes ethylene glycol and propylene glycol, and the defoamer includes organosilicone.

[0055] like Figures 3 to 5As shown, preferably, before performing step one, the method further includes: installing a planting shed on the planting ground; the planting shed includes a frame 1; a rainproof structure 2, set on the top and bottom of the frame 1, the rainproof structure 2 can be a film or glass; an insect-proof net 3, set on the outside of the frame 1, covering the outside of the frame 1; setting up the planting shed protects the citrus seedlings from insects and rain, while restricting the movement of psyllids, preventing them from escaping the orchard when spraying pesticides, and reducing the difficulty of planting and management in the prevention and control of citrus Huanglongbing.

[0056] like Figures 1 to 5 As shown, preferably, the planting shed further includes: a first linear moving device 4 and a spraying device 5; the first linear moving device 4 is aligned on both sides of the interior of the planting shed; the first linear moving device 4 is vertically arranged, and its movable end moves up and down in the height direction; the first linear moving device 4 includes a motor 41, a guide rail 42, a lead screw 43, and a connecting block 44; the lead screw 43 is rotatably mounted on the guide rail 42; the motor 41 is mounted on one end of the guide rail 42; the output end of the motor 41 is mechanically connected to one end of the lead screw 43, driving the lead screw 43 to rotate, optionally, the motor 41 is directly connected to the lead screw 43; the connecting block 44 is mounted on the lead screw 43, and the connecting block 44 is threadedly connected to the lead screw 43; the guide rail 44 is also provided with a rod, the rod being located on both sides of the lead screw 44; the spraying device 5 includes a housing 51, a hose 52, a first pipe 53, a second pipe 54, a circular pipe 55, a connecting plate 56, a nozzle 57, and a pump 58; the housing 51 is located inside the planting shed, the housing 51 is used for For holding water or medicine; both ends of the connecting plate 56 face the guide rail 42, and both ends of the connecting plate 56 are connected to the connecting block 44, and the connecting plate 56 rises and falls with the connecting block 44; the first pipe 53 is set on the connecting plate 56 and moves with the connecting plate 56; one end of the second pipe 54 is connected to the first pipe 53, and the other end faces the ground and is connected to the circular pipe 55, which is set horizontally and its axis is distributed vertically; the second pipe 54 is set vertically; multiple second pipes 54 are distributed at intervals along the length of the connecting plate 56, and the circular pipes 55 are distributed according to the position of the citrus tree; the nozzle 57 is connected to the circular pipe 55; multiple nozzles 57 are evenly distributed on the inner side of the circular pipe 55, and the nozzles 57 are atomizing nozzles; the pump 58 is set on the hose 52; one end of the hose 52 is connected to the box 51, and the other end is connected to one end of the first pipe 53; preferably, the end of the first pipe 53 away from the hose 52 is a closed structure; the first pipe 53 and the second pipe 54 are both rigid pipes.

[0057] Work style:

[0058] When applying pesticides or irrigating, the motor 41 and pump 58 are started. The motor 41 drives the lead screw 43 to rotate, and the connecting block 44 rotates with the lead screw 43, moving downwards. The connecting block 44 drives the connecting plate 56 to move downwards. The first pipe 53, the second pipe 54, and the annular pipe 55 descend with the connecting plate 56. The annular pipe 55 moves towards the citrus tree, with one annular pipe 55 corresponding to one citrus tree. The annular pipe 55 passes through the citrus tree, first from the top of the citrus tree to the bottom, and then from the bottom of the citrus tree... As the bottom returns to its original position, the circular pipe 55 passes through the citrus tree. The pump 58 draws water or pesticide from the water tank. The water or pesticide passes through the hose 52, the first pipe 53, and the second pipe 54 to reach the circular pipe 55. Finally, it is sprayed onto the citrus tree through the nozzle 57, spraying from top to bottom around the citrus tree so that both the top and bottom surfaces of the leaves are sprayed with the pesticide. In particular, it can effectively reduce the hiding of psyllids and increase the contact between psyllids and the pesticide, thus eliminating psyllids, especially in the prevention and control of Huanglongbing (HLB).

[0059] like Figure 1 and Figure 2 As shown, preferably, the planting shed also includes a second linear moving device 6; the second linear moving device 6 is located on both sides of the interior of the planting shed where the guide rail 42 is provided; the second linear moving device 6 has the same structure as the first linear moving device 4, and the second linear moving device 6 also includes a motor 61, a guide rail 62, a lead screw 63, and a connecting block 64; the connecting block 64 of the second linear moving device 6 is connected to one end of the guide rail 42 of the first linear moving device 4; the orientation of the guide rail 62 of the second linear moving device 6 is perpendicular to the guide rail 42 of the first linear moving device 4, and also perpendicular to the orientation of the connecting plate 34, that is, the second linear moving device 6 drives the first linear moving device 4 to move the first linear moving device 4. The linear moving device 4 and the connecting plate 34 move back and forth. The motor 61 of the second linear moving device 6 drives the lead screw 63 to rotate, and the connecting block 64 moves forward or backward. The guide rail 42 and the connecting plate 56 of the first linear moving device 4 also move forward or backward, improving the flexibility of the spraying device 5. Only one row of circular pipes 55 is provided. After one row of citrus trees is sprayed, the connecting plate 56 is moved by the second linear moving device 6, so that the row of circular pipes 55 moves. Then, the circular pipes 55 are raised and lowered by the first linear moving device 4 to spray the next row of citrus trees, reducing the number of circular pipes 55, thereby reducing the manufacturing cost of the spraying device 5.

[0060] like Figure 5As shown, preferably, the planting shed also includes a slide rail 7; the slide rail 7 is located on both sides inside the planting shed, on the same side as the second linear moving device 6; the slide rail 7 is set on the frame 1; the slide rail 7 is located above the second linear moving device 6; the orientation of the slide rail 7 is the same as the orientation of the second linear moving device 6; the guide rail 42 of the first linear moving device 4 has a corresponding slide rail 7 with a groove, and the slide rail 42 of the first linear moving device 4 is slidably connected to the slide rail 7 through the groove; the slide rail 7 is located above the second linear moving device 6, and the sliding connection between the slide rail 7 and the slide rail 42 increases the stability of the guide rail 42 of the first linear moving device 4 and prevents the guide rail 42 from shaking during movement.

[0061] This embodiment also discloses a preparation method for the above-mentioned planting method for preventing and controlling citrus Huanglongbing and citrus canker, including the following steps:

[0062] Step 1: Add difenoconazole, fluopyram, flusilazole, dispersant, and wetting agent to a sand mill according to the specified ratio. Simultaneously, add 55% deionized water to the sand mill and mix. Grind until the particle size is less than 6 micrometers. The dispersant includes at least one of lignin sulfur salt, polycarboxylate, and polyoxyethylene ether phosphate. The wetting agent includes at least one of resin polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, and sodium lauryl ether sulfate.

[0063] Step 2: Filter the material ground in Step 1, then add thickener, antifreeze, preservative, defoamer and remaining deionized water to the filtered material and stir. The thickener includes at least one of xanthan gum, carboxymethyl cellulose, magnesium aluminum silicate and polyvinyl alcohol; the antifreeze includes at least one of ethylene glycol, propylene glycol, glycerol and polyethylene glycol; the preservative includes at least one of isothiazolinone, paraformaldehyde, sodium benzoate and potassium sorbate; and the defoamer includes at least one of silicone oil, organosilicone and polysiloxane.

[0064] Step 3: Add the stirred material into the shearing vessel and shear at high speed for 2 hours. Discharge the material to obtain the bactericidal agent.

[0065] Example 2:

[0066] Compared with Example 1, the difference lies in steps one, four, and five; the rest are the same.

[0067] Step 1: Soil management. Spread 15 kg of fungicide and 19 kg of insecticide per acre of planting land, then plow to a depth of 22 cm, break up the soil that has been turned up, then irrigate, cover with a transparent film and let it stand for 12 days. After the standing period, remove the film, plow, dry, and ridge.

[0068] Step 2: Planting. Dig planting pits on the ridges and plant citrus seedlings in the pits.

[0069] Step 3: Weed the ridges. We can lay weed control cloth or other materials on the ridges to prevent weeds from growing and to avoid psyllids hiding in the weeds.

[0070] Step 4: During the spring shoots, summer shoots, autumn shoots, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 13 days. If the disease enters a severe outbreak period, spray the aforementioned insecticide every 8 days.

[0071] Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the fungicide every 17 days; during the summer shoot period, autumn shoot period, and after flowering, spray the citrus trees with the fungicide every 8 days.

[0072] Step Six: Clean the orchard regularly, pruning diseased, overgrown, and weak branches, and regularly removing fallen leaves, fallen fruit, and dead branches.

[0073] Example 3:

[0074] Compared with Example 1, the difference lies in the fact that steps one, four, and five are different;

[0075] Step 1: Soil management. Spread 20 kg of fungicide and 20 kg of insecticide per acre of planting land, then plow to a depth of 30 cm, break up the soil that has been turned up, then irrigate, cover with a transparent film and let it stand for 16 days. After the standing period, remove the film, then plow, dry, and ridge.

[0076] Step 2: Planting. Dig planting pits on the ridges and plant citrus seedlings in the pits.

[0077] Step 3: Weed the ridges. We can lay weed control cloth or other materials on the ridges to prevent weeds from growing and to avoid psyllids hiding in the weeds.

[0078] Step 4: During the spring shoots, summer shoots, autumn shoots, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 15 days. If the disease enters a severe outbreak period, spray the aforementioned insecticide every 10 days.

[0079] Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the fungicide every 20 days; during the summer shoot period, autumn shoot period, and after flowering, spray the citrus trees with the fungicide every 15 days.

[0080] Step Six: Clean the orchard regularly, pruning diseased, overgrown, and weak branches, and regularly removing fallen leaves, fallen fruit, and dead branches.

[0081] Example 4:

[0082] Compared with Example 1, the difference lies in the different weight percentages of the raw materials for the bactericide and insecticide in this application. The raw materials for the bactericide, by weight percentage, include 10% zinc thiazole, 25% orange peel oil, 2% dispersant, 3% wetting agent, 0.3% thickener, 1% antifreeze, 0.1% preservative, 0.2% defoamer, and the balance being water. The raw materials for the insecticide, by weight percentage, include 6% pyriproxyfen, 7% pymetrozine, 17% azadirachtin, 3% dispersant, 0.1% thickener, 1% antifreeze, 0.4% defoamer, and the balance being water.

[0083] Example 5:

[0084] Compared with Example 1, the difference lies in the different weight percentages of the raw materials for the bactericide and insecticide in this application. The raw materials for the bactericide, by weight percentage, include 25% zinc thiamethoxam, 10% orange peel essential oil, 8% dispersant, 7% wetting agent, 2% thickener, 5% antifreeze agent, 0.8% preservative, 1.2% defoamer, and the balance being water. The raw materials for the insecticide, by weight percentage, include 18% pyriproxyfen, 3% pymetrozine, 9% azadirachtin, 7% dispersant, 3% thickener, 4% antifreeze agent, 2% defoamer, and the balance being water.

[0085] Example 6:

[0086] Compared with Example 1, the difference lies in the weight percentage of the raw materials in the insecticide of this application. The raw materials of the insecticide, by weight percentage, include 6% pyriproxyfen, 9% pymetrozine, 17% azadirachtin, 5% dispersant, 4% thickener, 3% antifreeze, 1% defoamer, and the balance being water.

[0087] Example 7:

[0088] Compared with Example 1, the difference lies in the weight percentage of the raw materials in the insecticide of this application. The raw materials of the insecticide, by weight percentage, include 13% pyriproxyfen, 12% pymetrozine, 5% azadirachtin, 6% dispersant, 2% thickener, 2% antifreeze, 1.3% defoamer, and the balance being water.

[0089] Comparative Example 1:

[0090] Compared with Example 1, the difference is that the formulation of the bactericide is different. The bactericide, by mass percentage, includes the following components: 18% zinc thiazole, 4% dispersant, 5% wetting agent, 1.1% thickener, 3% antifreeze, 0.4% preservative, and 0.7% defoamer; that is, it does not contain orange peel essential oil.

[0091] Comparative Example 2:

[0092] Compared with Example 1, the difference is that the formulation of the bactericide is different. The bactericide, by mass percentage, includes the following components: 17% orange peel essential oil, 4% dispersant, 5% wetting agent, 1.1% thickener, 3% antifreeze agent, 0.4% preservative, and 0.7% defoamer, that is, it does not contain thiazole zinc.

[0093] Comparative Example 3:

[0094] Compared with Example 1, the difference is that the formulation of the insecticide is different. The insecticide, by weight percentage, includes the following components: 10% pyriproxyfen, 8% pymetrozine, 5% dispersant, 5% stabilizer, 2% thickener, 3% antifreeze, and 1% defoamer, that is, it does not contain azadirachtin.

[0095] Comparative Example 4:

[0096] Compared with Example 1, the difference is that the formulation of the insecticide is different. The insecticide, by weight percentage, includes the following components: 10% pyriproxyfen, 12% azadirachtin, 5% dispersant, 5% stabilizer, 2% thickener, 3% antifreeze, and 1% defoamer, that is, it does not contain pymetrozine.

[0097] Comparative Example 5:

[0098] Compared with Example 1, the difference is that the formulation of the insecticide is different. The insecticide, by weight percentage, includes the following components: 8% pymetrozine, 12% azadirachtin, 5% dispersant, 2% thickener, 3% antifreeze, and 1% defoamer, that is, it does not contain pyriproxyfen.

[0099] Comparative Example 6:

[0100] The difference between this example and Example 1 is that the insecticide used is different; this comparative example uses high-efficiency cypermethrin for insect control.

[0101] Comparative Example 7:

[0102] The difference between this example and Example 1 is that the bactericide used is different; this comparative example uses copper oxychloride for sterilization.

[0103] Comparative Example 8:

[0104] The difference from Example 1 is that no bactericides or insecticides were used in this comparative example; no bactericides or insecticides were applied.

[0105] Comparative Example 9:

[0106] The difference between this comparative example and Comparative Example 8 is that this comparative example does not have a planting shed installed.

[0107] Experimental Design:

[0108] This invention employs a total of 10 experimental groups: experimental group (Example 1), control group 1 (comparative example 1), control group 2 (comparative example 2), control group 3 (comparative example 3), control group 4 (comparative example 4), control group 5 (comparative example 5), control group 6 (comparative example 6), control group 7 (comparative example 7), blank group (comparative example 8), and control group 8 (comparative example 9). A total of 10 plots are involved, with each experimental group corresponding to one plot. Each plot is planted with 50 citrus seedlings (all seedlings have undergone PCR testing, and both citrus canker and Huanglongbing were negative).

[0109] Test location: Luowei Town, Longwuming District, Nanning City, Guangxi Province.

[0110] Planting time: February 2021.

[0111] Determination of incidence and control efficacy of Huanglongbing and Citrus canker in citrus seedlings

[0112] After the experiment, 10 leaves were collected from each fruit tree in each experimental group for indoor PCR testing of Huanglongbing (HLB) and Citrus canker (the specific principle is to use instruments to detect whether the DNA sequence of the pathogen in the citrus is the same as the DNA sequence of Huanglongbing and Citrus canker; if they are the same, it can be determined that the tree has been infected; if they are different, it indicates that the tree is not infected). The number of infected trees was counted, and the incidence rate and control effect were calculated using the following formula:

[0113] Huanglongbing incidence rate (%) = Number of infected Huanglongbing plants / Total number of plants in the plot × 100%

[0114] Huanglongbing control efficacy (%) = (Huanglongbing incidence rate in the blank group - Huanglongbing incidence rate in the control group) / Incidence rate in the blank group × 100%

[0115] Incidence rate of peptic ulcer disease (%) = Number of plants with peptic ulcer disease / Total number of plants in the plot × 100%

[0116] The efficacy rate of peptic ulcer prevention and treatment (%) = (incidence rate of peptic ulcer in the blank group - incidence rate of peptic ulcer in the control group) / incidence rate in the blank group × 100%

[0117] Results Analysis

[0118] The results of the pathogenesis examination of Huanglongbing and peptic ulcer disease in each experimental group are shown in Table 1 and Table 2. Table 1 shows the pathogenesis examination and control effect of Huanglongbing in each experimental group, and Table 2 shows the pathogenesis examination and control effect of peptic ulcer disease in each experimental group.

[0119] Table 1

[0120] Number of plants infected with peptic ulcer disease Total number of plants Incidence rate of peptic ulcer disease (%) Prevention and control efficacy (%) experimental group 3 50 6％ 92.9％ Blank group 42 50 84％ - Control group 1 24 50 48％ 42.9％ Control group 2 25 50 50％ 40.5％ Control group 3 4 50 8％ 90.5％ Control group 4 3 50 6％ 92.9％ Control group 5 4 50 8％ 90.5％ Control group 6 5 50 10％ 88.1％ Control group 7 29 50 58％ 30.1％ control group 8 43 50 86％ -2.3％

[0121] Table 2

[0122] Number of Huanglongbing (HLB) infected plants Total number of plants Huanglongbing incidence rate (%) Prevention and control efficacy (%) experimental group 1 50 2％ 97.7％ Blank group 43 50 86％ - Control group 1 1 50 2％ 97.7％ Control group 2 2 50 4％ 95.3％ Control group 3 23 50 46％ 46.5％ Control group 4 24 50 48％ 44.2％ Control group 5 26 50 52％ 37.6％ Control group 6 21 50 42％ 51.2％ Control group 7 2 50 4％％ 95.3％ control group 8 49 50 98％ -13.9％

[0123] As shown in Table 1, for the prevention and control of citrus canker, the incidence of citrus canker was much lower when using the fungicide of the present invention (Example 1, Comparative Example 3, Comparative Example 4 and Comparative Example 5) than when using conventional fungicides (Comparative Example 7), and the control effect was much higher than that of conventional fungicides. At the same time, the incidence of citrus canker was much lower when using the fungicide of the present invention than when using thiazole zinc and citrus peel essential oil alone, and the control effect was much higher than when using thiazole zinc and citrus peel essential oil alone (Comparative Example 1 and Comparative Example 2). The synergistic effect of thiazole zinc and citrus peel essential oil after being combined in a certain proportion resulted in a low incidence of canker and a high control effect.

[0124] As shown in Table 2, for the control of Huanglongbing (HLB), the insecticides of this invention (Example 1, Comparative Example 1, and Comparative Example 2) resulted in significantly lower HLB incidence rates compared to conventional insecticides (Comparative Example 6), and the control effect was also much higher than that of conventional insecticides. Furthermore, compared to Comparative Examples 3, 4, and 5, the combination of pyriproxyfen, pymetrozine, and azadirachtin in specific proportions produced a synergistic effect, effectively reducing the incidence of HLB and improving the control efficacy.

[0125] As shown in Tables 1 and 2, using planting sheds for citrus cultivation can reduce the incidence of citrus Huanglongbing (HLB) to a certain extent. The main reason is that planting sheds are relatively enclosed spaces, which prevent psyllids from entering from outside the shed and limit their activity inside. At the same time, the spraying device 5 can spray pesticide solution on the top and bottom surfaces of citrus leaves, effectively reducing psyllid hiding, increasing the contact between psyllids and pesticide solution, and further eliminating psyllids.

[0126] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A planting method for preventing and controlling Huanglongbing and Citrus Canker, characterized in that, Includes the following steps: Step 1: Soil management. Spread 10-20 kg of fungicide and 8-20 kg of insecticide per acre of planting land, then plow to a depth of 15-30 cm, break up the soil that has been turned up, then irrigate, cover with a transparent film and let it stand for 8-16 days. After the standing period, remove the film, plow, dry, and ridge. Step 2: Planting. Dig planting pits on the ridges and plant citrus seedlings in the pits. Step 3: Weed the ridges; Step 4: During the spring shoots, summer shoots, autumn shoots, and late autumn shoots of citrus trees, spray the citrus trees with the aforementioned insecticide every 10-15 days. If the disease enters a severe outbreak period, spray the aforementioned insecticide every 7-10 days. Step 5: During the spring shoot period of citrus trees, spray the citrus trees with the fungicide every 15-20 days. During the summer shoot, autumn shoot, and post-flowering period of citrus trees, spray the citrus trees with the fungicide every 10-15 days. Step Six: Clean the orchard regularly, pruning diseased branches, overgrown branches, and weak branches, while also regularly removing fallen leaves, fallen fruit, and dead branches; The active ingredients of the bactericide include zinc thiazole and orange peel essential oil; the bactericide, by mass percentage, comprises the following components: zinc thiazole 10-25%, orange peel essential oil 10-21%, dispersant 2-8%, wetting agent 3-7%, thickener 0.3-2%, antifreeze 1-5%, preservative 0.1-0.8%, defoamer 0.2-1.2%, with the balance being water; The active ingredients of the insecticide include pyriproxyfen, pymetrozine, and azadirachtin; the insecticide, by mass percentage, comprises the following components: pyriproxyfen 10-18%, pymetrozine 3-12%, azadirachtin 5-17%, dispersant 3-7%, thickener 0.1-3%, antifreeze 1-4%, defoamer 0.4-2%, with the balance being water.

2. The planting method for preventing and controlling citrus Huanglongbing and citrus canker according to claim 1, characterized in that, The bactericide, by weight percentage, comprises 18% zinc thiazole, 17% orange peel essential oil, 4% dispersant, 5% wetting agent, 1.1% thickener, 3% antifreeze, 0.4% preservative, 0.7% defoamer, and the remainder is water.

3. The planting method for preventing and controlling citrus Huanglongbing and citrus canker according to claim 1, characterized in that, The insecticide, by weight percentage, comprises 10% pyriproxyfen, 8% pymetrozine, 12% azadirachtin, 5% dispersant, 2% thickener, 3% antifreeze, 1% defoamer, and the remainder is water.

4. The planting method for preventing and controlling citrus Huanglongbing and citrus canker according to any one of claims 1-3, characterized in that, Before performing step one, the procedure also includes: Install planting sheds on the planting land; The planting shed includes: Skeleton (1); Rainproof structure (2) is provided on the top and bottom surfaces of the frame (1); Insect-proof net (3) is set on the outside of the frame (1) and covers the outside of the frame (1).

5. The planting method for preventing and controlling citrus Huanglongbing and citrus canker according to claim 4, characterized in that, The planting shed also includes: a first linear moving device (4) and a spraying device (5); the first linear moving device (4) is aligned on both sides of the interior of the planting shed; the first linear moving device (4) is vertically arranged; the first linear moving device (4) includes a motor (41), a guide rail (42), a lead screw (43) and a connecting block (44); the lead screw (43) is rotatably mounted on the guide rail (42); the motor (41) is mounted on one end of the guide rail (42); the output end of the motor (41) is mechanically connected to one end of the lead screw (43) to drive the lead screw (43) to rotate; the connecting block (44) is mounted on the lead screw (43); The spraying device (5) includes a housing (51), a hose (52), a first pipe (53), a second pipe (54), a circular pipe (55), a connecting plate (56), a nozzle (57), and a pump (58); the connecting plate (56) is connected to the connecting block (44); both ends of the connecting plate (56) face the guide rail (42); the first pipe (53) is mounted on the connecting plate (56); one end of the second pipe (54) is connected to the first pipe (53), and the other end faces the ground and is connected to the circular pipe. Pipe (55) is connected; the second pipe (54) is set vertically; multiple second pipes (54) are distributed at intervals along the length direction of the connecting plate (56); the annular pipe (55) is set horizontally; the nozzle (57) is connected to the annular pipe (55); multiple nozzles (57) are distributed at intervals on the inner side of the annular pipe (55); the pump (58) is set on the hose (52); one end of the hose (52) is connected to the box (51), and the other end is connected to one end of the first pipe (53).

6. The planting method for preventing and controlling citrus Huanglongbing and citrus canker according to claim 5, characterized in that, The planting shed also includes a second linear moving device (6); the second linear moving device (6) is located on both sides inside the planting shed where the guide rail (42) is provided; the second linear moving device (6) has the same structure as the first linear moving device (4); the second connecting block (64) of the second linear moving device (6) is connected to one end of the guide rail (42) of the first linear moving device (4); the orientation of the second guide rail (62) of the second linear moving device (6) is perpendicular to the guide rail (42) of the first linear moving device (4).

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