A composition capable of preventing and assisting in the treatment of type II diabetes, and its preparation method and application

Abstract

The invention discloses a composition that can prevent and assist in the treatment of type II diabetes, including the following raw materials by weight: 60-75 parts of green tea, 15-25 parts of mulberry leaves, 10-18 parts of polygonatum, 10-15 parts of white shouwu, 8-14 parts of scutellaria, 6-12 parts of bitter melon, 5-10 parts of kudzu root, 4-8 parts of corn silk, 3-6 parts of cyclocarya paliurus, 2-6 parts of bitter buckwheat, 2-5 parts of black-footed spear. With green tea as the tea base, ten Chinese medicinal materials are matched, and scientific and reasonable proportioning is carried out, and the obtained composition has a significant hypoglycemic effect, and the blood lipid level of diabetic mice can also be adjusted, and there is the effect of preventing and assisting the treatment of type II diabetes, wherein the tea fragrance of green tea can improve the taste of Chinese medicinal materials, and is easily accepted by people. In the preparation process, Chinese medicinal materials are combined and fermented with composite bacterial liquid, which can not only improve taste and smell, but also enhance nutritional value, improve the content and activity of effective ingredients, and thus enhance drug efficacy.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicinal tea preparations, and in particular to a composition capable of preventing and assisting in the treatment of type II diabetes, and a preparation method and application thereof. Background Art

[0002] The incidence and prevalence of diabetes are on the rise worldwide. Diabetes is a chronic disease characterized by hyperglycemia, caused by absolute or relative insulin deficiency and utilization disorders. The disease is mainly divided into three types: type 1, type 2 and gestational diabetes. The cause is mainly attributed to the combined effects of genetic and environmental factors, including decreased insulin secretion caused by pancreatic islet cell dysfunction, or the body's insensitivity to insulin, or both, which prevents glucose in the blood from being effectively utilized and stored.

[0003] The typical symptoms of type 2 diabetes are called "three mores and one less", which refers to drinking more, eating more, urinating more and losing weight; but in the early stage of the disease, there may be no symptoms or mild symptoms. As the disease progresses, the symptoms of hyperglycemia will become more obvious. The cause of type 2 diabetes is complex. Anyone who cannot be clearly diagnosed as other types of diabetes (type 1, special type diabetes, gestational diabetes) can be temporarily classified as type 2 diabetes. The reasonable treatment strategy for type 2 diabetes is "comprehensive", which not only includes hypoglycemic treatment, but also requires lowering blood pressure, regulating blood lipids, anti-platelet, controlling weight and improving lifestyle according to the individual complications of the patient. When blood sugar cannot be controlled by lifestyle adjustments, drug treatment will be started, including sulfonylureas, glinides, biguanides, thiazolidinediones, α-glucosidase inhibitors, etc. These drugs are usually accompanied by certain side effects such as gastrointestinal discomfort, such as nausea, diarrhea and heartburn. Long-term use is prone to drug resistance, not only causing greater damage to the liver and kidneys. Therefore, early prevention and treatment are particularly important. At present, more and more attempts are being made to use plant Chinese herbal medicines for treatment in clinical practice.

[0004] Traditional Chinese medicine is usually composed of a variety of Chinese herbs, which can comprehensively regulate the body, improve the overall metabolic state, and slow down the progression of the disease by affecting physiological processes such as blood sugar levels, insulin sensitivity, and insulin secretion. Compared with some Western medicines, traditional Chinese medicine may have fewer toxic side effects and relatively less impact on liver and kidney function. In addition to symptomatic treatment, Chinese herbal medicines have many other health benefits, such as relieving fatigue, improving sleep, regulating emotions, etc., which can improve the quality of life of patients. However, traditional Chinese medicine is usually composed of a variety of herbs, with complex ingredients, it is difficult to identify the active ingredients, and it is difficult to control the dosage. Due to factors such as planting conditions, harvesting time, and processing methods, the efficacy of the same Chinese medicinal materials may vary significantly, affecting the treatment effect. Some Chinese medicines taste bitter, which may affect the patient's acceptance. Therefore, it is urgently necessary to develop a Chinese medicine composition for the prevention and auxiliary treatment of type 2 diabetes that can solve the above problems. Summary of the invention

[0005] In view of this, the present invention proposes a composition capable of preventing and assisting in the treatment of type II diabetes and a preparation method and application thereof to solve the above problems.

[0006] The technical solution of the present invention is achieved in this way:

[0007] A composition for preventing and assisting in the treatment of type II diabetes comprises the following raw materials by weight: 60-75 parts of green tea, 15-25 parts of mulberry leaves, 10-18 parts of polygonatum, 10-15 parts of radix scutellariae, 8-14 parts of scutellaria baicalensis, 6-12 parts of bitter melon, 5-10 parts of kudzu root, 4-8 parts of corn silk, 3-6 parts of cyclocarya paliurus, 2-6 parts of tartary buckwheat and 2-5 parts of smilax china.

[0008] Preferably, the composition for preventing and assisting in the treatment of type II diabetes comprises the following raw materials by weight: 70 parts of green tea, 20 parts of mulberry leaves, 15 parts of polygonatum, 12 parts of radix scutellariae, 10 parts of scutellaria baicalensis, 8 parts of bitter melon, 8 parts of kudzu root, 6 parts of corn silk, 5 parts of cyclocarya paliurus, 4 parts of buckwheat, and 3 parts of smilax china.

[0009] Green tea is unfermented tea. Due to its characteristics, it retains more natural substances in fresh leaves. Tea polyphenols and caffeine retain more than 85% of fresh leaves, chlorophyll retains about 50%, and vitamin loss is also less, which forms the characteristics of green tea "clear soup, green leaves, strong astringent taste". It has special effects on anti-aging, anti-cancer, anti-cancer, sterilization, anti-inflammation, etc., which are beyond the reach of fermented tea.

[0010] Mulberry leaf, a Chinese medicine name, is the dried leaves of Morus alba L., also known as domestic mulberry, jing mulberry, mulberry tree, yellow mulberry leaf, etc. It tastes bitter and sweet, is cold in nature, and has the effects of dispersing wind-heat, clearing the lungs and moistening dryness, calming the liver and improving eyesight, cooling blood and stopping bleeding.

[0011] Huangjing, a Chinese medicine name, is the dried rhizome of Polygonatum kingianum Coll.et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua, a plant of the Liliaceae family. It tastes sweet and is neutral in nature. It has the effects of replenishing qi and yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys.

[0012] Bai Shou Wu, a Chinese medicine, is the root of Cynanchum wilfordii (Maxim.) Hemsl., a plant of the Apocynaceae family. It tastes sweet, slightly bitter, and is slightly warm in nature. It has the effects of nourishing the liver and kidneys, strengthening the tendons and bones, strengthening the spleen and stomach, and detoxifying.

[0013] Bitter melon, a Chinese medicine, is the fruit of Momordica charantia L., a plant of the Cucurbitaceae family. It tastes bitter and is cold in nature. It has the effects of dispelling summer heat, clearing away heat, improving eyesight, and detoxifying.

[0014] Pueraria root, a Chinese medicine, is the dried root of Pueraria montana var.lobata (Willdenow) Maesen & S.M.Almeida ex Sanjappa & Predeep, a plant of the Leguminosae family. It is commonly known as wild kudzu. It tastes sweet, spicy and cool. It has the effects of relieving muscle and fever, clearing rashes, promoting body fluid and quenching thirst, raising yang and stopping diarrhea, promoting blood circulation and activating collaterals, and detoxifying alcohol.

[0015] Corn silk, a Chinese medicine, is the style and stigma of Zea mays L., a plant of the Gramineae family. It tastes sweet and light, and is neutral in nature. It has the effects of diuresis, swelling, liver clearing and gallbladder promoting.

[0016] Cyclocarya paliurus, a Chinese medicine, is the bark, root or leaf of Cyclocarya paliurus (Batal.) Iljin. [Pterocarya paliurus Batal.; P.micropaliurus Tsoong] of the Juglandaceae family. It tastes slightly bitter and is warm in nature. It has the effects of dispelling wind, relieving inflammation and pain, killing insects and relieving itching.

[0017] Tartary buckwheat, a Chinese medicine, is a plant of the Polygonaceae family, Fagopyrum tataricum (L.) Gaertn., and its root tubers are used as medicine. It is dug up in autumn, washed, and dried. It has the effects of regulating qi and relieving pain, strengthening the spleen and removing dampness.

[0018] Mianbixie, a Chinese medicine name, is the dried rhizome of Dioscorea spongiosa JQXi.M.Mizunoet W.LZhao or Dioscorea futschauensis Uline ex R.Kunth of the Dioscoreaceae family. It tastes bitter and is neutral in nature. It has the effects of removing dampness, turbidity, wind and numbness.

[0019] Wujiaoqiang, a Chinese medicine name. It is the whole herb or root of Adiantum flabellulatum L., a plant of the genus Adiantum in the family Adiantumaceae. It tastes bitter and pungent, and is cool in nature. It has the effects of clearing away heat and dampness, detoxifying and dispersing nodules.

[0020] The present invention also provides a method for preparing a composition capable of preventing and assisting in the treatment of type II diabetes, comprising the following steps:

[0021] S1. Green tea processing

[0022] S1.1, Picking: Pick one bud and two leaves of fresh leaves;

[0023] S1.2, Sun-drying: The picked tea leaves are dried in the sun for 36-48 hours;

[0024] S1.3, Drying: Drying the tea leaves after sun-drying;

[0025] S1.4. Weigh the dried green tea by weight and crush it to obtain powder A;

[0026] S2. Chinese medicine processing

[0027] S2.1, Lactobacillus plantarum and Bacillus subtilis joint fermentation:

[0028] Weigh mulberry leaves, polygonatum, radix polygonati multiflori, and radix scutellariae by weight, mix and grind to obtain a grind, add the grind, Lactobacillus plantarum liquid, Bacillus subtilis liquid, and cold boiled water into a fermentation tank, stir evenly, ferment at 30-35° C. for 7-10 days under anaerobic conditions to obtain a fermentation liquid, squeeze and filter, and spray-dry to obtain a powder B;

[0029] S2.2, Aspergillus niger and Monascus co-fermentation:

[0030] Weigh bitter melon, kudzu root, schizonepeta tenuifolia, and tartary buckwheat according to weight, mix and grind to obtain a grind, add the grind, Aspergillus niger solution, Monascus purpurogenum solution, and cold boiled water into a fermentation tank, stir evenly, and ferment at 25-30° C. for 7-10 days under aerobic conditions to obtain a fermentation liquid, squeeze and filter, and spray dry to obtain a powder C;

[0031] S2.3, white rot fungi and Lactobacillus plantarum combined fermentation:

[0032] Weigh corn silk and Cyclocarya paliurus by weight, mix and grind to obtain a grind, add the grind, white rot fungus liquid, Lactobacillus plantarum liquid and boiled water into a fermentation tank, stir evenly, ferment at 25-30° C. for 7-10 days under aerobic conditions to obtain a fermentation liquid, squeeze and filter, spray dry to obtain powder D;

[0033] S3. Mix powder A, powder B, powder C and powder D, and homogenize them to obtain a composite material.

[0034] Preferably, the drying process in S1.3 is low-temperature baking at a temperature of 40-50° C. to maintain its natural aroma and taste.

[0035] Preferably, the total amount of the S2.1 Lactobacillus plantarum and Bacillus subtilis bacterial liquid is 3-5wt% of the mass of the crushed material, and the mass ratio of the Lactobacillus plantarum and Bacillus subtilis bacterial liquid is 1:2.

[0036] Preferably, the total amount of the Aspergillus niger and Monascus liquid in S2.2 is 3-5wt% of the mass of the crushed material, and the mass ratio of the Aspergillus niger and Monascus liquid is 1:1.

[0037] Preferably, the total amount of the S2.3 white rot fungi and Lactobacillus plantarum bacterial liquid is 3-5wt% of the mass of the crushed material, and the mass ratio of the white rot fungi and Lactobacillus plantarum bacterial liquid is 2:1.

[0038] Preferably, the mass volume g / mL ratio of the crushed material to cold boiled water is 1:4-6.

[0039] Lactobacillus plantarum is the most commonly used bacteria in food fermentation. It can produce lactic acid, improve the flavor and taste of food, and has a probiotic effect. Bacillus subtilis can secrete a variety of enzymes, has strong protein and starch degradation capabilities, and is relatively safe in food fermentation. Lactobacillus plantarum and yeast are used to co-ferment mulberry leaves, Polygonatum sibiricum, Cynanchum multiflorum, and Rhizoma Atractylodis Macrocephalae to enhance antioxidant capacity and functional ingredients, while improving taste.

[0040] Aspergillus niger can secrete a variety of hydrolases, such as cellulase, amylase and protease, which help degrade plant cell walls and release beneficial components. Monascus can produce a variety of bioactive substances during the fermentation process, such as lovastatin and pigments, which have lipid-lowering and antioxidant effects. The use of Aspergillus niger and Monascus in the combined fermentation of bitter melon, kudzu root, schizonepeta tenuifolia and buckwheat can increase their soluble dietary fiber and active ingredients, and enhance their health functions and biological activities.

[0041] White rot fungi are wood decay fungi that can degrade lignin and cellulose, releasing polyphenols and other bioactive substances. The combined fermentation of corn silk and Cyclocarya paliurus with white rot fungi and Lactobacillus plantarum can effectively decompose lignin and cellulose, increase soluble dietary fiber and polyphenols, and improve taste and flavor.

[0042] The present invention also provides an application of a composition capable of preventing and assisting in treating type II diabetes in the preparation of a product capable of preventing and assisting in treating type II diabetes.

[0043] Preferably, the product comprises tea.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] (1) The present invention provides a composition for preventing and assisting in the treatment of type II diabetes. The composition is prepared by using green tea as the base tea and combining it with ten kinds of Chinese medicinal materials in a scientific and reasonable proportion. The obtained composition has a significant blood sugar lowering effect and can also regulate the blood lipid level of diabetic mice. It has the efficacy of preventing and assisting in the treatment of type II diabetes. The tea aroma of green tea can improve the taste of Chinese medicinal materials and is easily accepted by people.

[0046] (2) The present invention provides a composition for preventing and assisting in the treatment of type II diabetes, wherein the Chinese medicinal materials are co-fermented with a composite bacterial solution, which can not only improve the taste and smell, but also enhance the nutritional value, increase the content and activity of the medicinal ingredients, and thus enhance the efficacy. DETAILED DESCRIPTION

[0047] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0048] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0049] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0050] Lactobacillus plantarum, Latin name Lactobacillus plantarum, was purchased from Shanghai Collection Biotechnology Center, number SHBCC D16021.

[0051] Bacillus subtilis, Latin name Bacillus subtilis, was purchased from Shanghai Biotechnology Collection Center, number SHBCC D52909.

[0052] Aspergillus niger, Latin name Aspergillus niger, was purchased from Shanghai Collection Biotechnology Center, No. SHBCCD22460.

[0053] Monascus anka, Latin name Monascus anka, was purchased from Shanghai Collection Biotechnology Center, number SHBCCD68022.

[0054] White rot fungus, Latin name Phanerochaetcchrysosporium, was purchased from Shanghai Collection Biotechnology Center, number SHBCC D82075.

[0055] Example 1

[0056] A composition for preventing and assisting in the treatment of type II diabetes comprises the following raw materials by weight: 60 parts of green tea, 25 parts of mulberry leaves, 10 parts of polygonatum, 15 parts of radix cynanchifolia, 14 parts of scutellaria baicalensis, 6 parts of bitter melon, 10 parts of kudzu root, 8 parts of corn silk, 3 parts of cyclocarya paliurus, 2 parts of tartary buckwheat and 5 parts of rhizoma scutellariae.

[0057] The preparation method of the composition capable of preventing and assisting in the treatment of type II diabetes comprises the following steps:

[0058] S1. Green tea processing

[0059] S1.1, Picking: Pick one bud and two leaves of fresh leaves;

[0060] S1.2, Sun drying: The picked tea leaves are dried in the sun for 36 hours;

[0061] S1.3 Drying: The sun-dried tea leaves are dried by low-temperature baking at a temperature of 45±5℃;

[0062] S1.4. Weigh the dried green tea by weight and crush it to obtain powder A;

[0063] S2. Chinese medicine processing

[0064] S2.1, Lactobacillus plantarum and Bacillus subtilis joint fermentation:

[0065] Weigh mulberry leaves, polygonatum, white shouwu and black scorpion according to weight, mix and crush to obtain a crushed material, add the crushed material, Lactobacillus plantarum liquid, Bacillus subtilis liquid and cold boiled water into a fermentation tank, wherein the total amount of Lactobacillus plantarum and Bacillus subtilis liquid is 5wt% of the mass of the crushed material, the mass ratio of Lactobacillus plantarum to Bacillus subtilis liquid is 1:2, and the effective viable bacteria count of each liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:6, stirred evenly, and fermented at 33℃ for 10 days under anaerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder B;

[0066] S2.2, Aspergillus niger and Monascus co-fermentation:

[0067] Weigh bitter melon, kudzu root, schizonepeta tenuifolia, and bitter buckwheat according to weight, mix and grind to obtain a grind, add the grind, Aspergillus niger liquid, Monascus liquid, and cold boiled water into a fermentation tank, wherein the total amount of Aspergillus niger and Monascus liquid is 5wt% of the grind, the mass ratio of Aspergillus niger liquid to Monascus liquid is 1:1, and the effective viable bacteria count of each bacterial liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:6, stirred evenly, and fermented at 27℃ for 10 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder C;

[0068] S2.3, white rot fungi and Lactobacillus plantarum combined fermentation:

[0069] Corn silk and Cyclocarya paliurus were weighed by weight, mixed, and crushed to obtain a crushed material, and the crushed material, white rot fungus liquid, Lactobacillus plantarum liquid, and cold boiled water were added to a fermentation tank, wherein the total amount of the white rot fungus and Lactobacillus plantarum liquid was 5wt% of the crushed material, the mass ratio of the white rot fungus and Lactobacillus plantarum liquid was 2:1, and the effective viable bacteria count of each liquid was 1×10 7cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:6, stirred evenly, and fermented at 27℃ for 10 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder D;

[0070] S3. Mix powder A, powder B, powder C and powder D, and homogenize them to obtain a composite material.

[0071] Example 2

[0072] A composition for preventing and assisting in the treatment of type II diabetes comprises the following raw materials by weight: 70 parts of green tea, 20 parts of mulberry leaves, 15 parts of polygonatum, 12 parts of radix cynanchifolia, 10 parts of scutellaria baicalensis, 8 parts of bitter melon, 8 parts of kudzu root, 6 parts of corn silk, 5 parts of cyclocarya paliurus, 4 parts of tartary buckwheat and 3 parts of smilax china.

[0073] The preparation method of the composition capable of preventing and assisting in the treatment of type II diabetes comprises the following steps:

[0074] S1. Green tea processing

[0075] S1.1, Picking: Pick one bud and two leaves of fresh leaves;

[0076] S1.2, Sun drying: The picked tea leaves are dried in the sun for 48 hours;

[0077] S1.3 Drying: The sun-dried tea leaves are dried by low-temperature baking at a temperature of 45±5℃;

[0078] S1.4. Weigh the dried green tea by weight and crush it to obtain powder A;

[0079] S2. Chinese medicine processing

[0080] S2.1, Lactobacillus plantarum and Bacillus subtilis joint fermentation:

[0081] Weigh mulberry leaves, polygonatum, white shouwu and black scorpion according to weight, mix and crush to obtain a crushed material, add the crushed material, Lactobacillus plantarum liquid, Bacillus subtilis liquid and cold boiled water into a fermentation tank, wherein the total amount of Lactobacillus plantarum and Bacillus subtilis liquid is 4wt% of the mass of the crushed material, the mass ratio of Lactobacillus plantarum to Bacillus subtilis liquid is 1:2, and the effective viable bacteria count of each liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:5, stirred evenly, and fermented at 33℃ for 10 days under anaerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder B;

[0082] S2.2, Aspergillus niger and Monascus co-fermentation:

[0083] Balsam pear, kudzu root, schizonepeta tenuifolia, and bitter buckwheat were weighed by weight, mixed and crushed to obtain a crushed material, and the crushed material, Aspergillus niger liquid, Monascus liquid, and cold boiled water were added to a fermentation tank, wherein the total amount of Aspergillus niger and Monascus liquid was 4wt% of the crushed material, the mass ratio of Aspergillus niger liquid to Monascus liquid was 1:1, and the effective viable bacteria count of each bacterial liquid was 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:5, stirred evenly, and fermented at 27℃ for 10 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder C;

[0084] S2.3, white rot fungi and Lactobacillus plantarum combined fermentation:

[0085] Corn silk and Cyclocarya paliurus were weighed by weight, mixed, and crushed to obtain a crushed material, and the crushed material, white rot fungus liquid, Lactobacillus plantarum liquid, and cold boiled water were added to a fermentation tank, wherein the total amount of the white rot fungus and Lactobacillus plantarum liquid was 4wt% of the crushed material, the mass ratio of the white rot fungus and Lactobacillus plantarum liquid was 2:1, and the effective viable bacteria count of each liquid was 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:5, stirred evenly, and fermented at 27℃ for 10 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder D;

[0086] S3. Mix powder A, powder B, powder C and powder D, and homogenize them to obtain a composite material.

[0087] Example 3

[0088] A composition for preventing and assisting in the treatment of type II diabetes comprises the following raw materials by weight: 75 parts of green tea, 15 parts of mulberry leaves, 18 parts of polygonatum, 10 parts of radix cynanchifolia, 8 parts of scutellaria baicalensis, 12 parts of bitter melon, 5 parts of kudzu root, 4 parts of corn silk, 6 parts of cyclocarya paliurus, 6 parts of tartary buckwheat, and 2 parts of smilax china.

[0089] The preparation method of the composition capable of preventing and assisting in the treatment of type II diabetes comprises the following steps:

[0090] S1. Green tea processing

[0091] S1.1, Picking: Pick one bud and two leaves of fresh leaves;

[0092] S1.2, Sun drying: The picked tea leaves are dried in the sun for 48 hours;

[0093] S1.3 Drying: The sun-dried tea leaves are dried by low-temperature baking at a temperature of 45±5℃;

[0094] S1.4. Weigh the dried green tea by weight and crush it to obtain powder A;

[0095] S2. Chinese medicine processing

[0096] S2.1, Lactobacillus plantarum and Bacillus subtilis joint fermentation:

[0097] Weigh mulberry leaves, polygonatum, white shouwu and black scorpion according to weight, mix and crush to obtain a crushed material, add the crushed material, Lactobacillus plantarum liquid, Bacillus subtilis liquid and cold boiled water into a fermentation tank, wherein the total amount of Lactobacillus plantarum and Bacillus subtilis liquid is 3wt% of the mass of the crushed material, the mass ratio of Lactobacillus plantarum to Bacillus subtilis liquid is 1:2, and the effective viable bacteria count of each liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:4, stirred evenly, and fermented at 33℃ for 7 days under anaerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder B;

[0098] S2.2, Aspergillus niger and Monascus co-fermentation:

[0099] Weigh bitter melon, kudzu root, schizonepeta tenuifolia, and bitter buckwheat according to weight, mix and grind to obtain a grind, add the grind, Aspergillus niger liquid, Monascus liquid, and cold boiled water into a fermentation tank, wherein the total amount of Aspergillus niger and Monascus liquid is 3wt% of the grind, the mass ratio of Aspergillus niger liquid to Monascus liquid is 1:1, and the effective viable bacteria count of each bacterial liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:4, stirred evenly, and fermented at 27℃ for 7 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder C;

[0100] S2.3, white rot fungi and Lactobacillus plantarum combined fermentation:

[0101] Corn silk and Cyclocarya paliurus were weighed by weight, mixed, and crushed to obtain a crushed material, and the crushed material, white rot fungus liquid, Lactobacillus plantarum liquid, and cold boiled water were added to a fermentation tank, wherein the total amount of the white rot fungus and Lactobacillus plantarum liquid was 3wt% of the crushed material, the mass ratio of the white rot fungus and Lactobacillus plantarum liquid was 2:1, and the effective viable bacteria count of each liquid was 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:4, stirred evenly, and fermented at 27℃ for 7 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder D;

[0102] S3. Mix powder A, powder B, powder C and powder D, and homogenize them to obtain a composite material.

[0103] Comparative Example 1

[0104] This example provides a composition for preventing and assisting in the treatment of type II diabetes. Compared with Example 2, the difference is that this example does not contain Radix Cynanchii, and the weight portion missing from Radix Cynanchii is supplemented by the proportion of other Chinese medicinal ingredients to the corresponding ingredients. The preparation method of the composition is basically the same as that of Example 2.

[0105] Comparative Example 2

[0106] This example provides a composition for preventing and assisting in the treatment of type II diabetes. Compared with Example 2, this example does not contain Herba Dioscoreae, and the weight portion missing from Herba Dioscoreae is supplemented by other Chinese medicinal ingredients to the corresponding components. The preparation method of the composition is basically the same as that of Example 2.

[0107] Comparative Example 3

[0108] This example provides a composition for preventing and assisting in the treatment of type II diabetes. Compared with Example 2, this example does not contain blackfoot ginseng, and the weight portion of other Chinese medicinal ingredients missing from blackfoot ginseng is supplemented to the corresponding ingredients. The preparation method of the composition is basically the same as that of Example 2.

[0109] Comparative Example 4

[0110] This example provides a composition that can prevent and assist in the treatment of type II diabetes. The raw material composition is the same as that in Example 2. The difference is that in the preparation method of the composition of this example, the combined fermentation of S2.1 Lactobacillus plantarum and Bacillus subtilis is changed to a single fermentation of Bacillus subtilis, and the total bacterial liquid content remains unchanged.

[0111] Comparative Example 5

[0112] This example provides a composition that can prevent and assist in the treatment of type II diabetes. The raw material composition is the same as that of Example 2. Compared with Example 2, the difference is that in the preparation method of the composition of this example, the combined fermentation of Aspergillus niger and Monascus in S2.2 is changed to a single fermentation of Aspergillus niger, and the total bacterial liquid content remains unchanged.

[0113] Comparative Example 6

[0114] This example provides a composition that can prevent and assist in the treatment of type II diabetes. The raw material composition is the same as that of Example 2. Compared with Example 2, the difference is that in the preparation method of the composition of this example, the combined fermentation of S2.3 white rot fungi and Lactobacillus plantarum is changed to a single fermentation of Lactobacillus plantarum, and the total bacterial liquid content remains unchanged.

[0115] Comparative Example 7

[0116] This example provides a composition for preventing and assisting in the treatment of type II diabetes. The raw material composition is the same as that of Example 2. Compared with Example 2, the difference is that in the preparation method of the composition of this example, S2.2, the combined fermentation of Aspergillus niger and Monascus is replaced by the combined fermentation of Lactobacillus plantarum and Bacillus subtilis, that is:

[0117] S2.2, Lactobacillus plantarum and Bacillus subtilis co-fermentation:

[0118] Weigh bitter melon, kudzu root, schizonepeta tenuifolia, and bitter buckwheat according to weight, mix and grind to obtain a grind, add the grind, Lactobacillus plantarum liquid, Bacillus subtilis liquid, and cold boiled water into a fermentation tank, wherein the total amount of Lactobacillus plantarum and Bacillus subtilis liquid is 3wt% of the weight of the grind, the mass ratio of Lactobacillus plantarum to Bacillus subtilis liquid is 1:2, and the effective viable count of each liquid is 1×10 7 cfu / mL, the mass volume g / mL ratio of the crushed material to cold boiled water was 1:4, stirred evenly, and fermented at 33°C for 10 days under aerobic conditions to obtain the fermentation liquid, which was squeezed and filtered, and spray-dried to obtain powder C.

[0119] Test Example: Efficacy test of a composition capable of preventing and assisting in the treatment of type II diabetes

[0120] 120 male SD rats were subjected to adaptive feeding for 1 week. The rats were randomly divided into two groups: blank group (10 rats) and modeling group (110 rats). The rats in the blank group were fed with basic feed, while the rats in the modeling group were given high-fat and high-sugar feed, with the feed formula of 15% lard, 25% sucrose, 3% cholesterol, 1% bile salt, 6% soy protein powder and 50% basic feed, and were fed continuously for one month. On the 30th day, the rats were fasted for 12 hours. In a 4℃ ice bath, a 1% STZ (streptozotocin) solution was freshly prepared with a citric acid-sodium citrate buffer solution containing 0.1 mol / L concentration of streptozotocin and pH 4.4. The rats in the modeling group were intraperitoneally injected with 1% streptozotocin solution at a dose of 35 mg / kg, while the rats in the blank group were injected with an equal volume of 0.1 mol / L citric acid-sodium citrate buffer solution (pH 4.4). 72 hours after injection, blood was collected from the tail vein and blood glucose levels were measured.

[0121] If the random blood glucose value is higher than 16.7mmol / L, the diabetes model is considered to be successful. If the standard value is not reached, 1% STZ solution is injected again the next day, and the dose is adjusted to 30mg / kg. Random grouping and drug treatment: Once the blood glucose is stable, the rats with successful modeling are randomly divided into 11 groups, with 10 rats in each group: model group, embodiment 1-3 group, and comparative example 1-7 group. The embodiment group and the comparative example group were given corresponding drugs at a dose of 0.01g / kg, and administered by gavage. The blank group and the model group were given an equal amount of 0.9% NaCl solution. The drug treatment lasted for one month. After the administration, fast for 4 hours, then blood was collected from the tail vein, and blood glucose levels were measured using a blood glucose meter. Rats were anesthetized with chloral hydrate, blood was collected from the abdominal aorta, and the total cholesterol (TC) and triglyceride (TG) contents in serum were determined by the kit method after centrifugation. The results are shown in Table 1.

[0122] Table 1

[0123]

[0124]

[0125] Note: Compared with the blank control group, * P<0.05; compared with the model group, ** P<0.05; compared with Example 2 group, # P<0.05.

[0126] The results show that the Chinese medicine composition of the present invention has a significant hypoglycemic effect on diabetic mice and can regulate the TG / TC level of diabetic mice. The composition prepared in Examples 1-3 of the present invention has better effects than Comparative Examples 1-7.

[0127] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for preventing and assisting in the treatment of type II diabetes, characterized in that: The composition is composed of the following raw materials by weight: 60-75 parts of green tea, 15-25 parts of mulberry leaves, 10-18 parts of polygonatum, 10-15 parts of radix cynanchifolii, 8-14 parts of scutellaria baicalensis, 6-12 parts of bitter melon, 5-10 parts of kudzu root, 4-8 parts of corn silk, 3-6 parts of cyclocarya paliurus, 2-6 parts of tartary buckwheat, and 2-5 parts of blackfoot smilax china. The preparation method of the composition comprises the following steps: S1. Green tea processing S1.

1. Picking: Pick one bud and two leaves of fresh leaves; S1.2, Sun-drying: The picked tea leaves are dried in the sun for 36-48 hours; S1.3, Drying: Drying the tea leaves after sun-drying; S1.4, weigh the dried green tea according to weight, and crush it to obtain powder A; S2. Chinese medicine processing S2.1, Lactobacillus plantarum and Bacillus subtilis joint fermentation: Weigh mulberry leaves, polygonatum, radix polygonati multiflori, and radix scutellariae by weight, mix and grind to obtain a grind, add the grind, Lactobacillus plantarum liquid, Bacillus subtilis liquid, and cold boiled water into a fermentation tank, stir evenly, ferment at 30-35° C. for 7-10 days under anaerobic conditions to obtain a fermentation liquid, squeeze and filter, and spray-dry to obtain a powder B; S2.2, Aspergillus niger and Monascus co-fermentation: Weigh bitter melon, kudzu root, schizonepeta tenuifolia, and tartary buckwheat according to weight, mix and grind to obtain a grind, add the grind, Aspergillus niger solution, Monascus purpurogenum solution, and cold boiled water into a fermentation tank, stir evenly, and ferment at 25-30° C. for 7-10 days under aerobic conditions to obtain a fermentation liquid, squeeze and filter, and spray dry to obtain a powder C; S2.3, white rot fungi and Lactobacillus plantarum combined fermentation: Weigh corn silk and Cyclocarya paliurus by weight, mix and grind to obtain a grind, add the grind, white rot fungus liquid, Lactobacillus plantarum liquid and boiled water into a fermentation tank, stir evenly, ferment at 25-30° C. for 7-10 days under aerobic conditions to obtain a fermentation liquid, squeeze and filter, spray dry to obtain powder D; S3, mixing powder A, powder B, powder C and powder D, and homogenizing to obtain a composition; The total amount of the S2.1 Lactobacillus plantarum and Bacillus subtilis bacterial liquid is 3-5wt% of the mass of the crushed material, and the mass ratio of the Lactobacillus plantarum and Bacillus subtilis bacterial liquid is 1:2; The total amount of the Aspergillus niger and Monascus liquid in S2.2 is 3-5wt% of the crushed material, and the mass ratio of the Aspergillus niger and Monascus liquid is 1:1; The total amount of the S2.3 white rot fungi and Lactobacillus plantarum liquid is 3-5wt% of the mass of the crushed material, and the mass ratio of the white rot fungi to the Lactobacillus plantarum liquid is 2:

1.

2. The composition according to claim 1, characterized in that The composition is composed of the following raw materials by weight: 70 parts of green tea, 20 parts of mulberry leaves, 15 parts of polygonatum, 12 parts of radix scutellariae, 10 parts of scutellaria baicalensis, 8 parts of bitter melon, 8 parts of kudzu root, 6 parts of corn silk, 5 parts of cyclocarya paliurus, 4 parts of tartary buckwheat and 3 parts of blackfoot rhizome.

3. The composition according to claim 1, characterized in that The drying process in step S1.3 is low temperature baking, and the baking temperature is 40-50°C.

4. The composition according to claim 1, characterized in that The mass volume g / mL ratio of the crushed material to the cold boiled water in S2.1, S2.2 and S2.3 is 1:4-6 respectively.

5. Use of the composition according to any one of claims 1 to 4 in the preparation of medicines for preventing and assisting in the treatment of type II diabetes.

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