Method for preparing river mud for dyeing and finishing Xiangyun cotton by microbial fermentation

Abstract

The application discloses a method for preparing river mud for dyeing and finishing of Xiangyun cotton by microbial fermentation, and the method comprises the following steps: drying and powdering mature saponaria pericarp, mixing the saponaria pericarp powder with deionized water to form a suspension, mixing rhizopus, glutinous rice powder and deionized water to ferment, mixing the suspension with the fermented mixture, adding yeast, separating the precipitate from the fermentation liquid by using a vacuum filter, retaining the obtained precipitate, adding silkworm chrysalis powder, cocoon powder, rice powder and white sugar into the precipitate and stirring, adding bacillus subtilis, carbonyl iron powder and hydroxyl apatite and continuing to stir, and placing the mixture in a water bath pot to ferment, finally mixing the fermented mixture with substandard river mud for dyeing and finishing of Xiangyun cotton and carrying out dyeing and finishing. The saponaria powder, silkworm chrysalis powder, silk powder, carbonyl iron powder and hydroxyl apatite are deeply decomposed by bacillus subtilis and yeast, the method can repair the river mud for dyeing and finishing of Xiangyun cotton, and the problem that the river mud for dyeing and finishing of Xiangyun cotton is gradually reduced and the natural dyeing and finishing mud cannot meet the production of Xiangyun cotton is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to a method for preparing river mud for dyeing and finishing Xiangyun silk by microbial fermentation, and belongs to the field of textile printing and dyeing. BACKGROUND

[0002] Xiangyun silk is a kind of real silk fabric dyed and finished by traditional handcraft techniques, and is unique in South China. Xiangyun silk has a natural and environmentally friendly coating on its surface, and has excellent properties such as cool and comfortable, easy to wash and dry, long-wearing, crisp and not clingy, etc.

[0003] Xiangyun silk dyeing and finishing technique is a traditional handcraft technique in Shunde, Foshan, and has a history of several hundred years. In 2008, Xiangyun silk dyeing and finishing technique was listed in the national intangible cultural heritage list. The main steps of Xiangyun silk dyeing and finishing technique are to use the tuberous root of wild plant Dioscorea panthaica as dye, to repeatedly immerse and expose the gray silk, and then to coat a specific river mud for dyeing and finishing on the surface of the silk to "over-bleaching", and the processing is completed after the silk surface changes color.

[0004] The use of dyeing and finishing river mud for "over-bleaching" is the key link of Xiangyun silk dyeing and finishing technique. The dyeing and finishing river mud in the Pearl River Delta region mainly comes from the Lengjiao river in Shunde. Using river mud from other areas for "over-bleaching" treatment, the surface of the fabric has very low iron content, the fabric has dull luster, the coating is uneven in thickness, the color consistency is poor, the texture is too soft, the air permeability is not good, and it is difficult to meet the qualified Xiangyun silk standard. In recent years, affected by industrial pollution, climate change and other factors, the Lengjiao river in Shunde has been polluted to a certain extent, and the dyeing and finishing river mud that meets the requirements is decreasing, which cannot meet the needs of Xiangyun silk production and intangible cultural heritage protection. Research has found that the mechanism of Xiangyun silk dyeing and finishing "over-bleaching" may be 1. The high valence iron ions in the dyeing and finishing river mud react with tannic acid to generate black precipitate and condense on the surface of the silk; 2. There is a microbial microenvironment. The substandard dyeing and finishing river mud may lack iron elements, lack of corresponding microorganisms, and the microenvironment lacks organic metabolites. Based on previous research, a method for preparing Xiangyun silk dyeing and finishing river mud by microbial fermentation is provided through repeated experiments.

[0005] Gleditsia sinensis, also known as Gleditsia sinensis, is a deciduous tree or shrub of the genus Gleditsia in the family Leguminosae, and is originally from Asia. Gleditsia sinensis is rich in triterpenoid saponins, lignin, polysaccharides and other active ingredients, and has antibacterial, anti-inflammatory and antioxidant effects. The saponin content in Gleditsia sinensis fruit is the highest, about 15% to 30%. Gleditsia sinensis fermentation broth can be prepared by microbial fermentation.

[0006] Microbial fermentation is an effective method for producing a large amount of metabolites, correcting pH value and changing soil microenvironment. Rhizopus is a single cell fungus, and its enzyme system contains abundant amylase, including liquefied and saccharified amylase, can produce lactic acid, fumaric acid, succinic acid and trace alcohol, and can also produce aromatic ester substances, which can be used for the preparation of edible sweet wine and saccharified feed. Yeast is a single cell fungus, which can survive in aerobic and anaerobic environments, and belongs to facultative anaerobic bacteria. In the presence of oxygen, yeast decomposes sugar into carbon dioxide and water and grows faster. In the absence of oxygen, yeast decomposes sugar into alcohol and carbon dioxide. Bacillus subtilis is widely distributed in soil and decaying organic matter, and the bacterial body itself synthesizes alpha-amylase, protease, lipase, cellulase and other enzymes. At present, there is no report on the preparation of river mud for dyeing and finishing of Xiangyun cotton by microbial fermentation. Through repeated experiments, a method for preparing river mud for dyeing and finishing of Xiangyun cotton by combined fermentation of Rhizopus and yeast is explored. SUMMARY

[0007] In view of the fact that the river mud for dyeing and finishing of Xiangyun cotton is decreasing, and the natural dyeing and finishing mud cannot meet the actual situation of Xiangyun cotton production and protection of intangible cultural heritage, through experimental analysis and technical innovation, a method for preparing river mud for dyeing and finishing of Xiangyun cotton by microbial fermentation is explored.

[0008] The method for preparing river mud for dyeing and finishing of Xiangyun cotton by microbial fermentation comprises the following steps:

[0009] 1) The mature soap nuts are deseeded and the peel is retained, and then placed in an oven at a temperature of 40-50 DEG C for 36 hours for drying; the dried soap nut peel is ground into powder, and the powder is repeatedly filtered through a 55-65 mesh screen for 3 times, and then the unfiltered particles are repeatedly ground and filtered to remove impurities, to obtain soap nut peel powder for later use;

[0010] 2) The soap nut powder and deionized water are mixed according to a weight ratio of 1:10-15 to prepare a soap nut suspension;

[0011] 3) The Rhizopus, glutinous rice powder and deionized water are mixed according to a weight ratio of 1:500:1000, and then placed in a sealed container and placed in a water bath at a temperature of 30-35 DEG C for fermentation for 24 hours to obtain a fermentation liquor;

[0012] 4) The soap nut suspension prepared in step 2) and the fermentation liquor prepared in step 3) are mixed according to a ratio of 1:50 and stirred uniformly to obtain a soap nut fermentation liquor; the yeast and the soap nut fermentation liquor are mixed according to a weight ratio of 1:200 and stirred uniformly; the mixed liquid is placed in a water bath at a temperature of 30-35 DEG C for fermentation; after sufficient fermentation for 3-5 days, the fermentation liquor is obviously stratified, the bottom layer has precipitates, the upper layer is clear, and a rotten smell is emitted;

[0013] 5) Separating the precipitate and liquid in the fermentation liquid obtained in step 4) by using a vacuum filter and a cloth funnel with a pore size of 100 um, removing the liquid and retaining the obtained precipitate;

[0014] 6) Breaking open the dried silkworm cocoons, taking out the silkworm pupae, and powdering them. After powdering, the powder is repeatedly filtered through a 75-85 mesh screen for 3 times. Then, the unfiltered granular material is repeatedly powdered and filtered to remove impurities, thereby obtaining silkworm pupa powder. The silkworm cocoon shells are dried in an oven at 88-92℃ for 24 hours. After taking out, the shells are powdered and repeatedly filtered through a 75-85 mesh screen for 3 times. Then, the unfiltered granular material is repeatedly powdered and filtered to remove impurities, thereby obtaining silkworm silk powder. The silkworm pupa powder and the silkworm silk powder are mixed in equal proportions to obtain silkworm pupa-silk mixed powder.

[0015] 7) Mixing and stirring the precipitate prepared in step 5), rice powder, white sugar, and the silkworm pupa-silk mixed powder prepared in step 6) in a weight ratio of 1:1:0.2:0.5 to obtain a first mixture.

[0016] 8) Mixing the first mixture prepared in step 7), Bacillus subtilis, carbonyl iron powder, and hydroxyapatite in a weight ratio of 1:0.01:0.3:0.05, and stirring uniformly to obtain a second mixture.

[0017] 9) Sealing the second mixture obtained in step 8) and storing it in a water bath at 30-35℃ for fermentation. After 30 days of sufficient fermentation, a fermented mixture is obtained. After opening, it can be observed that the color of the precipitate changes from dark gray to brown black, and a rotten smell is emitted.

[0018] 10) Mixing and stirring the fermented mixture in step 9) with the off-specification river mud for dyeing and finishing of cotton yarn in a weight ratio of 1:10-15 to obtain newly prepared river mud for dyeing and finishing.

[0019] As a preferred embodiment, the mature soapberry in step 1) is seedless and the pericarp is dried in an oven at a temperature of 45℃ for 36 hours. The dried pericarp is powdered, and the powder is repeatedly filtered through a 60 mesh screen for 3 times. Then, the unfiltered granular material is repeatedly powdered and filtered to remove impurities, thereby obtaining soapberry pericarp powder for standby use.

[0020] As a preferred embodiment, the soapberry powder and deionized water are mixed in a weight ratio of 1:12 to prepare a soapberry suspension in step 2).

[0021] As a preferred embodiment, the Rhizopus, glutinous rice powder, and deionized water are mixed in a weight ratio of 1:500:1000 in step 3), and the mixture is placed in a sealed container and fermented in a water bath at 33℃ for 24 hours to obtain a fermentation liquid.

[0022] As preferred, in step 4), the soapnut suspension prepared in step 2) is mixed with the fermentation liquor prepared in step 3) at a ratio of 1:50 and stirred uniformly to obtain a soapnut fermentation liquor; the yeast and the soapnut fermentation liquor are configured at a ratio of 1:200 by weight and continue to be stirred uniformly; the mixed liquor is placed in a water bath at 33 DEG C for fermentation; after sufficient fermentation for 4 days, it is obvious that the fermentation liquor is stratified, the bottom layer has precipitates, the upper layer is clear, and a foul odor is emitted.

[0023] As preferred, in step 4), after the fermentation liquor is stratified, the PH value is between 3.5 and 5.2, which is weakly acidic, and there are a large number of metabolic products produced by fermentation in the liquid.

[0024] As preferred, in step 6), the dried silkworm chrysalis is broken open, the silkworm pupa inside is taken out and powdered, the powder is filtered repeatedly for 3 times through an 80-mesh screen, the granular material that has not passed through the screen is repeatedly powdered and filtered, and after impurities are removed, silkworm pupa powder is obtained; the silkworm chrysalis shell is dried in an oven at 90 DEG C for 24 hours, taken out and powdered, and filtered repeatedly for 3 times through an 80-mesh screen, the granular material that has not passed through the screen is repeatedly powdered and filtered, and after impurities are removed, silkworm silk powder is obtained; the silkworm pupa powder and the silkworm silk powder are mixed at an equal ratio to obtain silkworm pupa and silk mixed powder.

[0025] As preferred, in step 9), the second mixture obtained in step 8) is sealed and stored in a water bath at 33 DEG C for fermentation, and a fermentation mixture is obtained after sufficient fermentation for 30 days; after being opened, it is obvious that the color of the precipitates changes from dark gray to brown black, and a foul odor is emitted; whether the fermentation is completed can be determined by color and odor.

[0026] As preferred, in step 10), the fermentation mixture in step 9) is mixed with the substandard river mud for dyeing and finishing of cotton-polyester blended fabric at a ratio of 1:12 by weight to obtain newly prepared river mud for dyeing and finishing.

[0027] As preferred, the yeast, the rhizopus and the bacillus subtilis are used as fermentation strains; the soapnut, the rice and the white sugar are used as nutrients for fermentation; the carbonyl iron powder and the hydroxyl apatite are used for improving the microenvironment of the river mud during fermentation.

[0028] Beneficial effects: the present application realizes the repair of the river mud for dyeing and finishing of cotton-polyester blended fabric through deep fermentation and decomposition of the ginkgo biloba powder, the silkworm pupa powder, the silkworm silk powder, the carbonyl iron powder and the hydroxyl apatite by the bacillus subtilis and the yeast; through XRF detection, the content of iron elements on the surface of the fabric is less than 5% different from that of the fabric obtained by the traditional dyeing and finishing process of cotton-polyester blended fabric; and through electron microscope observation, the observation morphology is basically the same. The present application solves the problems that the river mud for dyeing and finishing of cotton-polyester blended fabric is decreasing, and the natural dyeing and finishing mud cannot meet the actual problems of protection of the cotton-polyester blended fabric and the intangible cultural heritage. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 is a picture of the silk crepe satin fabric repeatedly immersed in Dioscorea opposita Thunb juice and dyed black by the application, taken under a scanning electron microscope at 200 times magnification;

[0030] Figure 2 is a picture of the fabric dyed black by the application, taken under a scanning electron microscope at 200 times magnification. DETAILED DESCRIPTION

[0031] The following examples are further illustrations of the application and do not limit the application.

[0032] Example 1

[0033] The mature soap angle is deseeded and the peel is reserved. 1 kg of the peel is dried in an oven at a temperature of 50℃ for 36 hours. The dried soap angle peel is powdered and filtered repeatedly for 3 times through a 60-mesh screen. The particles that are not filtered through the screen are repeatedly powdered and filtered to remove impurities to obtain soap angle peel powder for later use. 1 kg of the soap angle powder and 10 kg of deionized water are mixed to prepare a soap angle suspension. 50 g of rhizopus, 25 kg of glutinous rice powder and 50 kg of deionized water are mixed and placed in a sealed container in a water bath at 35℃ for fermentation for 24 hours to obtain a fermentation liquid. The prepared soap angle suspension 1 kg and the prepared fermentation liquid 50 kg are mixed and stirred uniformly, and then 255 g of yeast is added and stirred uniformly. The mixed liquid is placed in a water bath at 35℃ for fermentation. After sufficient fermentation for 5 days, it is obvious that the fermentation liquid is stratified, with sediment at the bottom, clear liquid at the top, and a foul odor. The sediment and liquid in the fermentation liquid are separated by a vacuum filter and a large cloth funnel, and the obtained sediment is reserved for about 20 kg. The dried silkworm chrysalis is broken open, and the silkworm pupa inside is taken out and powdered. The powder is filtered repeatedly for 3 times through an 80-mesh screen, and the particles that are not filtered through the screen are repeatedly powdered and filtered to remove impurities to obtain silkworm pupa powder 10 kg for later use. The silkworm cocoon shell is dried in an oven at 90℃ for 24 hours, taken out and powdered, and filtered repeatedly for 3 times through an 80-mesh screen. The particles that are not filtered through the screen are repeatedly powdered and filtered to remove impurities to obtain silkworm silk powder 10 kg for later use. The 10 kg of silkworm pupa powder and the 10 kg of silkworm silk powder are mixed uniformly. The sediment 20 kg obtained after filtration, rice powder 20 kg, sugar 4 kg, silkworm pupa powder and silkworm silk powder 10 kg are mixed and stirred uniformly to obtain a dark mixture. The dark mixture 50 kg, bacillus subtilis 500 g, carbonyl iron powder 15 kg and hydroxylapatite 2.5 kg are mixed and stirred uniformly, and then sealed and stored in a large water bath at 35℃ for fermentation for 30 days. After opening, it is obvious that the color of the sediment changes from dark gray to brown black, and a foul odor is emitted. The dark gray sediment 50 kg is mixed and stirred uniformly with the off-specification river mud 500 kg for dyeing and finishing of the silk cloud to obtain newly prepared river mud for dyeing and finishing. The prepared river mud for dyeing and finishing is applied to the surface of the silk cloud after dyeing and finishing of the morinda officinalis to be "over black", and placed in a shaded and ventilated place to dry. Then the dark gray adherent on the surface of the silk is washed off, and the dried silk is obtained. From the appearance and color, the obtained silk product is no different from the silk cloud obtained by "over black" with the natural dyeing and finishing river mud. Through XRF detection, the iron element content on the surface of the fabric is less than 5% different from the iron element content on the surface of the fabric obtained by the traditional dyeing and finishing process of the silk cloud. There is no obvious difference in the microstructure observed by scanning electron microscope. Referring to Figure 1 is a picture taken under a scanning electron microscope at 200 times magnification after the over black dyeing of the true silk crepe satin fabric repeatedly immersed in the morinda officinalis juice by the present application. From the picture, it can be clearly seen that a thick layer is wrapped around the surface of the warp and weft yarns. Referring to Figure 2The mass ratio of iron element on the surface of the fabric after the dyeing by the application is 52.11% by XRF detection, slightly higher than the iron element content after the dyeing and finishing by the natural digging standard river mud, and the difference is less than 5%.

[0034] Example 2

[0035] The mature soap seed is de-seeded and the fruit skin is reserved. 100 g of the fruit skin is dried in an oven at 40℃ for 36 hours. The dried soap seed skin is ground into powder, and the powder is filtered through a 60-mesh screen for 3 times. The unfiltered particles are repeatedly ground into powder and filtered. After removing the impurities, the soap seed skin powder is obtained and reserved. 100 kg of soap seed powder and 1 kg of deionized water are mixed to prepare a soap seed suspension. 5 g of Rhizopus, 2.5 kg of glutinous rice powder and 5 kg of deionized water are mixed, and the mixture is placed in a sealed container and fermented in a water bath at 40℃ for 24 hours to obtain a fermentation liquid. 100 g of the prepared soap seed suspension and 5 kg of the prepared fermentation liquid are mixed and stirred uniformly, and then 25.5 g of yeast is added and stirred uniformly. The mixed liquid is placed in a water bath at 40℃ for fermentation. After 5 days of sufficient fermentation, it is obvious that the fermentation liquid is stratified, with precipitate at the bottom, clear supernatant, and foul odor. The precipitate and the liquid in the fermentation liquid are separated by a vacuum filter and a large cloth funnel, and about 2 kg of the obtained precipitate is reserved. The dried silkworm cocoons are broken open, and the silkworm chrysalis inside is taken out and ground into powder. The powder is filtered through an 80-mesh screen for 3 times. The unfiltered particles are repeatedly ground into powder and filtered. After removing the impurities, 1 kg of silkworm chrysalis powder is obtained and reserved. The silkworm cocoon shells are dried in an oven at 90℃ for 24 hours, and then ground into powder and filtered through an 80-mesh screen for 3 times. The unfiltered particles are repeatedly ground into powder and filtered. After removing the impurities, 1 kg of silkworm silk powder is obtained and reserved. 1 kg of silkworm chrysalis powder and 1 kg of silkworm silk powder are mixed uniformly. The precipitate obtained after filtration, 2 kg of rice powder, 400 g of white sugar, 1 kg of silkworm chrysalis powder and 1 kg of silkworm silk powder are mixed and stirred uniformly to obtain a dark mixture. 5 kg of the dark mixture, 50 g of Bacillus subtilis, 1.5 kg of carbonyl iron powder and 250 g of hydroxylapatite are mixed and stirred uniformly, and then the mixture is sealed and placed in a large water bath at 40℃ for fermentation for 30 days. After opening the seal, it is obvious that the color of the precipitate changes from dark gray to brown black, and foul odor is emitted. 5 kg of the dark gray precipitate is mixed and stirred uniformly with 50 kg of the river mud that does not meet the requirements of dyeing and finishing of silk yarn to obtain newly prepared river mud for dyeing and finishing. The prepared river mud for dyeing and finishing is applied to the surface of the silk yarn dyed and finished by Dioscorea cirrhosa Lour. and then “overdyed”, and then the silk yarn is placed in a shaded and ventilated place to dry. After washing off the dark gray adhesion on the surface of the silk yarn, the silk yarn is dried. From the appearance and color, the obtained silk yarn product is the same as the silk yarn dyed and finished by the natural river mud “overdyed”. Through XRF detection, the content of iron element on the surface of the fabric is less than 10% different from the content of iron element on the surface of the fabric obtained by the traditional dyeing and finishing process of silk yarn. There is no obvious difference in the microstructure observed by scanning electron microscopy.

[0036] The above examples are illustrative of the present application and are not limiting of the present application. Any modification of the present application that comes within the spirit and scope of the claims together with their equivalents are also intended to be within the scope of the present application.

Claims

1. A method for preparing river mud for dyeing and finishing of silk yarn by microbial fermentation, characterized in that, The process steps are: 1) The mature soap seed is removed from the seed and the peel is retained, and is placed in an oven at a temperature of 40-50°C for 36 hours to dry; the dried soap peel is powdered, and is repeatedly filtered through a 55-65 mesh screen for 3 times, and the particles that have not passed through the screen are repeatedly powdered and filtered, and after removing impurities, the soap peel powder is obtained for standby; 2) The soap powder and deionized water are mixed according to a weight ratio of 1:10-15 to prepare a soap suspension; 3) The Rhizopus, glutinous rice powder and deionized water are mixed according to a weight ratio of 1:500:1000, and are placed in a sealed container and are fermented in a water bath at 30-35°C for 24 hours to obtain a fermentation liquid; 4) The soap suspension prepared in step 2) and the fermentation liquid prepared in step 3) are mixed according to a ratio of 1:50 and are stirred uniformly to obtain a soap fermentation liquid; yeast and the soap fermentation liquid are mixed according to a weight ratio of 1:200 and are continuously stirred uniformly; the mixed liquid is placed in a water bath at 30-35°C for fermentation; after fully fermenting for 3-5 days, the fermentation liquid is obviously stratified, with sediment at the bottom, clear liquid at the top, and a foul odor; 5) The sediment and liquid obtained in step 4) are separated by a vacuum filter and a cloth funnel with a pore size of 100 um, the liquid is removed, and the obtained sediment is retained; 6) The dried silkworm cocoon is broken open, the silkworm pupa inside is taken out and is powdered, and after being powdered, the silkworm pupa is repeatedly filtered through a 75-85 mesh screen for 3 times, and the particles that have not passed through the screen are repeatedly powdered and filtered, and after removing impurities, the silkworm pupa powder is obtained; the silkworm cocoon shell is dried in an oven at 88-92°C for 24 hours, is taken out and is powdered, and is repeatedly filtered through a 75-85 mesh screen for 3 times, and the particles that have not passed through the screen are repeatedly powdered and filtered, and after removing impurities, the silkworm silk powder is obtained; the silkworm pupa powder and the silkworm silk powder are mixed in equal proportions to obtain a silkworm pupa and silk mixed powder; 7) The sediment prepared in step 5), rice powder, sugar, and the silkworm pupa and silk mixed powder prepared in step 6) are mixed and stirred uniformly according to a weight ratio of 1:1:0.2:0.5 to obtain a first mixture; 8) The first mixture prepared in step 7), Bacillus subtilis, carbonyl iron powder and hydroxyapatite are mixed according to a weight ratio of 1:0.01:0.3:0.05 and are stirred uniformly to obtain a second mixture; 9) The second mixture obtained in step 8) is sealed and stored in a water bath at 30-35°C for fermentation, and a fermentation mixture is obtained after fully fermenting for 30 days; after being opened, the sediment is obviously changed from dark gray to brown black, and a foul odor is emitted; 10) The fermentation mixture in step 9) and the off-specification river mud for dyeing and finishing of silk yarn are mixed and stirred uniformly according to a weight ratio of 1:10-15 to obtain newly prepared river mud for dyeing and finishing.

2. A process for the preparation of river mud for dyeing and finishing of Xiangyun cotton using microbial fermentation as claimed in claim 1, wherein, In step 1), the mature soap seed is removed from the seed and the peel is retained, and is placed in an oven at a temperature of 45°C for 36 hours to dry; the dried soap peel is powdered, and is repeatedly filtered through a 60 mesh screen for 3 times, and the particles that have not passed through the screen are repeatedly powdered and filtered, and after removing impurities, the soap peel powder is obtained for standby.

3. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 2), the soap nut powder and deionized water are mixed in a proportion of 1:12 by weight to prepare a soap nut suspension.

4. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 3), the Rhizopus, glutinous rice powder and deionized water are mixed in a proportion of 1:500:1000 by weight, and are placed in a closed container and fermented in a water bath at 33℃ for 24 hours to obtain a fermentation liquor.

5. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 4), the soap nut suspension prepared in step 2) and the fermentation liquor prepared in step 3) are mixed in a proportion of 1:50 and stirred uniformly to obtain a soap nut fermentation liquor; the yeast and the soap nut fermentation liquor are mixed in a proportion of 1:200 by weight and stirred uniformly; the mixed liquor is placed in a water bath at 33℃ for fermentation; after 4 days of sufficient fermentation, the fermentation liquor is obviously stratified, with sediment at the bottom, clear supernatant and a foul odor.

6. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 4), after the fermentation liquor is stratified, the pH value is between 3.5 and 5.2, and the liquid is weakly acidic, with a large amount of metabolic products produced by fermentation.

7. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 6), the dried silkworm cocoons are broken open, and the silkworm pupae inside are taken out and ground into powder; the powder is repeatedly filtered through an 80-mesh screen 3 times, and the particles that have not passed through the screen are repeatedly ground into powder and filtered; after the impurities are removed, silkworm pupa powder is obtained; the silkworm cocoon shells are dried in an oven at 90℃ for 24 hours, and then ground into powder and repeatedly filtered through an 80-mesh screen 3 times; after the impurities are removed, silkworm silk powder is obtained; the silkworm pupa powder and the silkworm silk powder are mixed in equal proportions to obtain silkworm pupa and silk mixed powder.

8. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 9), the second mixture obtained in step 8) is sealed and stored in a water bath at 33℃ for fermentation, and a fermentation mixture is obtained after 30 days of sufficient fermentation; after opening, the color of the precipitate changes from dark gray to brown black, and a foul odor is emitted; whether the fermentation is complete can be determined by color and odor.

9. The method for preparing river mud for dyeing and finishing Xiangyun cotton fabric by microbial fermentation according to claim 1, characterized in that, In step 10), the fermentation mixture in step 9) and the off-specification river mud for dyeing and finishing of cotton-polyester blended fabric are mixed in a proportion of 1:12 by weight and stirred uniformly to obtain newly prepared river mud for dyeing and finishing.

10. The method for preparing river mud for dyeing and finishing Xiangyun cotton using microbial fermentation according to any one of claims 1 to 9, characterized in that, The yeast, Rhizopus and Bacillus subtilis are used as fermentation strains; the soap nut, rice and sugar are used as nutrients for fermentation; the carbonyl iron powder and hydroxylapatite are used to improve the microenvironment of the river mud during fermentation.

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