A bioaerosol detection and sampling integrated machine and its use method

Abstract

The present invention relates to the technical field of aerosol devices, and specifically discloses a bioaerosol detection and sampling integrated machine and a method of using the same, including a sampling cup, a liquid storage bottle, a waste liquid bottle, an identification component and a sample tube, wherein a sampling fan is fixedly arranged at the upper end of the sampling cup, the left end of the sampling cup is connected to the liquid storage bottle, and a first three-way valve is fixedly arranged at the lower end of the sampling cup; a second three-way valve is fixedly arranged at the right end of the first three-way valve, the left end of the first three-way valve is connected to the waste liquid bottle, a burette is fixedly arranged at the lower end of the second three-way valve, the identification component is located at the lower end of the burette, and the right end of the second three-way valve is connected to the sample tube; the identification component includes an identification shell, a detection tray, a receiving tray, an instrument tray, a rotating shaft and a replacement component, and the detection tray is located inside the identification shell. The present invention can realize the fully automatic integrated operation of sampling, titration and biological species identification, realize automatic rotation card replacement, and perform identification in multiple channels at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of aerosol devices, and in particular to a bioaerosol detection and sampling integrated machine and a use method thereof. Background Art

[0002] Bioaerosols refer to particles suspended in the air released by living organisms, including microorganisms such as bacteria, fungi, viruses, pollen, spores, and parasite eggs. These bioaerosols not only have the physical and chemical properties of ordinary aerosols, but also have biological characteristics, and can enter the human body through air transmission, causing various health problems.

[0003] The existing technology still has the following areas for improvement: the existing sampling, titration, and biological species identification of bioaerosols usually require three instruments for separate detection, and staff are required to move the samples between different instruments. During the transportation process, the samples are easily contaminated, resulting in inaccurate sample results. In addition, the existing instruments can usually only detect one sample and cannot achieve simultaneous detection of multiple samples, which is time-consuming and labor-intensive. Summary of the invention

[0004] In order to solve the above problems existing in the prior art, the present invention provides a bioaerosol detection and sampling all-in-one machine and a method of using the same, which can realize fully automatic integrated operation of sampling, titration, and biological species identification, realize automatic rotation and card replacement, and perform multi-channel identification simultaneously.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A bioaerosol detection and sampling machine comprises a sampling cup, a liquid storage bottle, a waste liquid bottle, an identification component and a sample retention tube, wherein a sampling fan is fixedly arranged at the upper end of the sampling cup, the left end of the sampling cup is connected to the liquid storage bottle, and a first three-way valve is fixedly arranged at the lower end of the sampling cup;

[0007] A second three-way valve is fixedly disposed at the right end of the first three-way valve, the left end of the first three-way valve is connected to the waste liquid bottle, a burette is fixedly disposed at the lower end of the second three-way valve, the identification component is located at the lower end of the burette, and the right end of the second three-way valve is connected to the sample retention tube;

[0008] The identification component includes an identification shell, a detection disk, a receiving disk, an instrument disk, a rotating shaft and a replacement component, wherein the detection disk is located inside the identification shell, the detection disk, the receiving disk and the instrument disk are all sleeved on the rotating shaft, the detection disk is located above the receiving disk, the receiving disk is located above the instrument disk, and the detection disk is fixedly connected to the rotating shaft;

[0009] The lower end of the detection plate is evenly fixed with three movable slide rails, the circumference of the receiving plate is evenly fixed with three receiving sliders, the circumference of the instrument plate is evenly fixed with three instrument sliders, and the three receiving sliders and the three instrument sliders are respectively slidably connected to the three movable slide rails;

[0010] The receiving tray is provided with a reagent card, the instrument tray is evenly provided with six photoelectric probes, the detection tray is provided with six detection holes, the receiving tray is provided with six detection holes, and the six detection holes, the six detection holes and the six photoelectric probes are all aligned;

[0011] A slide groove is provided on the rotating shaft, the receiving plate and the instrument plate are slidably connected in the slide groove, the three receiving sliders and the three instrument sliders are all provided with moving motors, and a controller is fixedly provided inside the identification shell.

[0012] Furthermore, a first peristaltic pump is fixedly arranged between the sampling cup and the liquid storage bottle, and a second peristaltic pump is fixedly arranged between the sampling cup and the first three-way valve.

[0013] Furthermore, the upper surface of the detection disk and the upper surface of the identification housing are located on the same horizontal plane.

[0014] Furthermore, the detection disk is a hexagonal structure, the receiving disk and the instrument disk are both circular structures, a movable hole is provided on the reagent card, and the width of the movable hole is greater than the diameter of the rotating shaft.

[0015] Furthermore, the lower end of the rotating shaft is rotatably connected to the inner bottom of the identification shell through a rotating fixing member, a rotating disk is fixedly sleeved on the rotating shaft, a driving shaft is arranged inside the identification shell, a driving motor is fixedly arranged at the lower end of the driving shaft, and a chuck change disk is sleeved on the driving shaft.

[0016] Furthermore, a driving rod is fixedly provided at the lower end of the chuck changing disc, the chuck changing disc cooperates with the rotating disc, the driving rod cooperates with the rotating hole opened on the rotating disc, and the driving motor is fixedly connected to the inner bottom of the identification shell through a motor fixing part.

[0017] Furthermore, an adsorption hole is opened on the right side of the identification shell, and the replacement assembly includes two suction cups, two transverse slide rails and two longitudinal slide rails. The two transverse slide rails are located on the right side of the identification shell, and the two longitudinal slide rails are respectively slidably connected to the two transverse slide rails through transverse sliders, and an adsorption rod is fixedly provided on the upper end of the two suction cups.

[0018] Furthermore, the two adsorption rods are slidably connected to the two longitudinal slide rails through longitudinal sliders, and an adsorption motor is fixedly provided on the upper ends of the two adsorption rods, and the upper ends of the two longitudinal slide rails are connected through a connecting piece.

[0019] A method for using a bioaerosol detection and sampling machine comprises the following steps:

[0020] S1: sampling and titration operation;

[0021] The first peristaltic pump is started to move the liquid medium in the liquid storage bottle to the sampling cup, and the sampling fan is started to collect the aerosol particles in the sampling cup. After the aerosol particles are mixed with the liquid medium, the sample passes through the second peristaltic pump, the first three-way valve and the second three-way valve in sequence, and falls into the detection hole through the burette;

[0022] S2: identification operation;

[0023] The controller starts the driving motor, the driving shaft drives the card changing disk to rotate, the driving rod rotates to each rotating hole in turn, drives the rotating disk to rotate, the receiving disk moves upward to contact with the lower end of the detection disk, the sample drips onto the reagent card through the detection hole, the instrument disk moves upward, and the photoelectric probe performs identification operation on the reagent card through the detection hole;

[0024] After the test, if the result is positive, the sample retention operation is performed, and the controller starts the second peristaltic pump to transport the excess sample in the sampling cup to the sample retention tube through the first three-way valve and the second three-way valve in sequence;

[0025] If the result is negative, the controller starts the second peristaltic pump to transfer the excess sample in the sampling cup to the waste bottle through the first three-way valve;

[0026] S3: cleaning operation;

[0027] The controller performs a cleaning procedure to transfer the liquid medium in the liquid storage bottle to the sampling cup for cleaning, and the cleaned liquid flows into the waste liquid bottle through the second peristaltic pump and the first three-way valve to perform the next round of sampling operation;

[0028] The controller starts, the longitudinal slider drives the two adsorption rods to move upward, the transverse slider drives the two longitudinal slide rails to move to the left, the two suction cups move to the reagent card, the longitudinal slider drives the two adsorption rods to move downward, the adsorption motor starts, the suction cup adsorbs the reagent card, the transverse slider drives the two longitudinal slide rails to move to the right, the used reagent card is moved to the outside, and then the new reagent card is adsorbed on the suction cup, and the new reagent card is moved to the receiving tray.

[0029] The beneficial effects of the present invention are:

[0030] (1) By arranging a sampling fan, a sampling cup, a liquid storage bottle, a first three-way valve and a second three-way valve, the technical effect that can be achieved is that the sampling fan is fixedly arranged at the upper end of the sampling cup, the left end of the sampling cup is connected to the liquid storage bottle, the first three-way valve is fixedly arranged at the lower end of the sampling cup, the second three-way valve is fixedly arranged at the right end of the first three-way valve, the burette is fixedly arranged at the lower end of the second three-way valve, and the identification component is located at the lower end of the burette;

[0031] The first peristaltic pump is started to move the liquid medium in the liquid storage bottle to the sampling cup, and the sampling fan is started to collect the aerosol particles in the sampling cup. After the aerosol particles are mixed with the liquid medium, the sample passes through the second peristaltic pump, the first three-way valve and the second three-way valve in sequence, and falls into the detection hole through the burette. The sampling fan and the sampling cup can automatically suck the aerosol particles and mix them with the liquid medium. The first three-way valve and the second three-way valve realize the fully automatic integrated operation of the three parts of sampling, titration and biological species identification. The liquid storage bottle is used to store the liquid medium and can mix and clean the sampling cup.

[0032] (2) By setting the identification component, the technical effect that can be achieved is that three movable slide rails are evenly fixedly arranged at the lower end of the detection disk, three receiving sliders are evenly fixedly arranged at the circumference of the receiving disk, and three instrument sliders are evenly fixedly arranged at the circumference of the instrument disk. The three receiving sliders and the three instrument sliders are respectively slidably connected to the three movable slide rails, a reagent card is arranged on the receiving disk, six photoelectric probes are evenly arranged on the instrument disk, six detection holes are opened on the detection disk, and six detection holes are opened on the receiving disk. The six detection holes, six detection holes and six photoelectric probes are all aligned;

[0033] The controller starts the driving motor, the active shaft drives the card changing disk to rotate, the driving rod rotates to each rotating hole in turn, drives the rotating disk to rotate, the receiving disk moves upward to contact with the lower end of the detection disk, the sample drips onto the reagent card through the detection hole, the instrument disk moves upward, and the photoelectric probe identifies the reagent card through the detection hole. The identification component adopts a hexahedral structure to rotate and automatically change the card, realizing six full-process automated operations, multi-channel integrated cards, and realizing synchronous multi-channel identification. There is no need for manual instrument replacement, and multiple samples can be automatically detected synchronously.

[0034] (3) By arranging the sampling cup, the waste liquid bottle, the sample retention tube, the first three-way valve and the second three-way valve, the technical effect that can be achieved is that the first three-way valve is fixedly arranged at the lower end of the sampling cup, the second three-way valve is fixedly arranged at the right end of the first three-way valve, the left end of the first three-way valve is connected to the waste liquid bottle, and the right end of the second three-way valve is connected to the sample retention tube;

[0035] After the test, if the result is positive, the sample retention operation is performed, and the controller starts the second peristaltic pump to transfer the excess sample in the sampling cup to the sample retention tube through the first three-way valve and the second three-way valve in sequence; if the result is negative, the controller starts the second peristaltic pump to transfer the excess sample in the sampling cup to the waste liquid bottle through the first three-way valve, and the negative samples that need to be retained are processed through the first three-way valve and the second three-way valve, and the positive samples are processed as waste liquid. There is no need for manual operation by the staff, and the samples can be automatically judged and operated, which is safer and more convenient.

[0036] (4) By setting the replacement components, the technical effect that can be achieved is that the two transverse slide rails are both located on the right side of the identification shell, the two longitudinal slide rails are respectively connected to the two transverse slide rails through transverse sliders, the upper ends of the two suction cups are respectively fixed with an adsorption rod, the two adsorption rods are respectively connected to the two longitudinal slide rails through longitudinal sliders, the upper ends of the two adsorption rods are respectively fixed with an adsorption motor, and the upper ends of the two longitudinal slide rails are connected through connecting pieces;

[0037] The controller performs a cleaning procedure to transport the liquid medium in the liquid storage bottle to the sampling cup for cleaning. The cleaned liquid flows into the waste liquid bottle through the second peristaltic pump and the first three-way valve to perform the next round of sampling operation; the controller starts, the longitudinal slider drives the two adsorption rods to move upward, the transverse slider drives the two longitudinal slide rails to move to the left, the two suction cups move to the reagent card, the longitudinal slider drives the two adsorption rods to move downward, the adsorption motor starts, the suction cup adsorbs the reagent card, the transverse slider drives the two longitudinal slide rails to move to the right, the used reagent card is moved to the outside, and then the new reagent card is adsorbed on the suction cup, and the new reagent card is moved to the receiving plate. The replacement component can replace the reagent card to prevent the staff from manually touching the contaminated reagent card, which is safer and more convenient. The waste liquid bottle collects the waste liquid, making the cleaning of the sampling cup more automated and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to facilitate understanding by those skilled in the art, the present invention is further described below with reference to the accompanying drawings.

[0039] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0040] Figure 2 A front cross-sectional view of the detection state of the identification component in the present invention;

[0041] Figure 3 A front cross-sectional view showing a replacement state of an identification component in the present invention;

[0042] Figure 4 A top view of a replacement component in the present invention;

[0043] Figure 5 A partial cross-sectional view of the detection state of the identification component in the present invention;

[0044] Figure 6 A partial cross-sectional view of the replacement status of the identification component in the present invention;

[0045] Figure 7 A top view of the detection disk of the present invention;

[0046] Figure 8 is a top view of the reagent card of the present invention;

[0047] Fig. 9 A top view of the rotating disk and the chuck changing disk in the present invention in a stopped state;

[0048] Fig.10 A top view of the rotating disk and the chuck changing disk in the present invention in the starting state;

[0049] Fig.11 It is a front cross-sectional view of the rotating disk and the chuck changing disk in the present invention in a stopped state;

[0050] Fig.12 It is a front cross-sectional view of the rotating disk and the chuck changing disk in the present invention in the starting state;

[0051] Description of main component symbols:

[0052] In the figure: 1, sampling fan; 2, sampling cup; 3, first peristaltic pump; 4, liquid storage bottle; 5, second peristaltic pump; 6, waste liquid bottle; 7, first three-way valve; 8, second three-way valve; 9, identification component; 10, sample tube;

[0053] 91. Identification shell; 92. Detection disk; 93. Rotating shaft; 94. Receiver disk; 95. Instrument disk; 96. Rotating disk; 97. Rotating fixture; 98. Card changer; 99. Driving motor; 910. Active shaft; 911. Driving rod; 912. Suction cup; 913. Adsorption rod; 914. Longitudinal slider; 915. Longitudinal slide rail; 916. Transverse slide rail; 917. Transverse slider; 918. Photoelectric probe; 919. Adsorption motor; 920. Connector; 921. Instrument slider; 922. Moving slide rail; 923. Receiver slider; 924. Detection hole; 925. Reagent card; 926. Controller; 927. Motor fixture; 928. Detection hole. DETAILED DESCRIPTION

[0054] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application; obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application without making creative work are within the scope of protection of the present application.

[0055] In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0056] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "set / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0057] Reference Figures 1 to 12 , which is a bioaerosol detection and sampling integrated machine disclosed in the present invention, includes a sampling cup 2, a liquid storage bottle 4, a waste liquid bottle 6, an identification component 9 and a sample tube 10, a sampling fan 1 is fixedly provided at the upper end of the sampling cup 2, the left end of the sampling cup 2 is connected to the liquid storage bottle 4, and a first three-way valve 7 is fixedly provided at the lower end of the sampling cup 2.

[0058] A second three-way valve 8 is fixedly provided at the right end of the first three-way valve 7, the left end of the first three-way valve 7 is connected to the waste liquid bottle 6, a burette is fixedly provided at the lower end of the second three-way valve 8, the identification component 9 is located at the lower end of the burette, and the right end of the second three-way valve 8 is connected to the sample tube 10.

[0059] The sampling fan 1 and the sampling cup 2 can automatically inhale aerosol particles and mix them with the liquid medium. The first three-way valve 7 and the second three-way valve 8 realize the fully automatic integrated operation of sampling, titration, and biological species identification. The liquid storage bottle 4 is used to store the liquid medium and can mix and clean the sampling cup 2.

[0060] The negative samples that need to be retained are processed through the first three-way valve 7 and the second three-way valve 8, and the positive samples are treated as waste liquid. There is no need for manual operation by the staff, and the samples can be automatically judged and operated, which is safer and more convenient.

[0061] The identification component 9 includes an identification shell 91, a detection disk 92, a receiving disk 94, an instrument disk 95, a rotating shaft 93 and a replacement component. The detection disk 92 is located inside the identification shell 91. The detection disk 92, the receiving disk 94 and the instrument disk 95 are all mounted on the rotating shaft 93. The detection disk 92 is located above the receiving disk 94. The receiving disk 94 is located above the instrument disk 95. The detection disk 92 is fixedly connected to the rotating shaft 93.

[0062] Three movable slide rails 922 are evenly fixedly set at the lower end of the detection plate 92, three receiving sliders 923 are evenly fixedly set at the circumference of the receiving plate 94, and three instrument sliders 921 are evenly fixedly set at the circumference of the instrument plate 95. The three receiving sliders 923 and the three instrument sliders 921 are slidably connected to the three movable slide rails 922 respectively.

[0063] A reagent card 925 is arranged on the receiving tray 94, six photoelectric probes 918 are evenly arranged on the instrument tray 95, six detection holes 924 are opened on the detection tray 92, and six detection holes 928 are opened on the receiving tray 94. The six detection holes 924, six detection holes 928 and six photoelectric probes 918 are all aligned.

[0064] A slide groove is provided on the rotating shaft 93, and the receiving plate 94 and the instrument plate 95 are slidably connected in the slide groove. The three receiving sliders 923 and the three instrument sliders 921 are all provided with moving motors, and a controller 926 is fixedly provided inside the identification shell 91.

[0065] A first peristaltic pump 3 is fixedly disposed between the sampling cup 2 and the liquid storage bottle 4, and a second peristaltic pump 5 is fixedly disposed between the sampling cup 2 and the first three-way valve 7. The upper surface of the detection disk 92 and the upper surface of the identification housing 91 are located on the same horizontal plane.

[0066] The detection tray 92 is a hexagonal structure, the receiving tray 94 and the instrument tray 95 are both circular structures, and a moving hole is opened on the reagent card 925, and the width of the moving hole is greater than the diameter of the rotating shaft 93.

[0067] The lower end of the rotating shaft 93 is rotatably connected to the inner bottom of the identification shell 91 through a rotating fixing member 97. A rotating disk 96 is fixedly sleeved on the rotating shaft 93. A driving shaft 910 is arranged inside the identification shell 91. A driving motor 99 is fixedly arranged at the lower end of the driving shaft 910. A chuck change disk 98 is sleeved on the driving shaft 910.

[0068] A driving rod 911 is fixedly provided at the lower end of the chuck changing plate 98, the chuck changing plate 98 cooperates with the rotating plate 96, the driving rod 911 cooperates with the rotating hole opened on the rotating plate 96, and the driving motor 99 is fixedly connected to the inner bottom of the identification shell 91 through the motor fixing part 927.

[0069] The identification component 9 adopts a hexahedral structure to rotate and automatically change the card, realizing six times of full-process automated operation, a multi-channel integrated card, and realizing synchronous multi-channel identification. There is no need for manual instrument replacement, and multiple samples can be automatically and synchronously detected.

[0070] An adsorption hole is provided on the right side of the identification shell 91. The replacement assembly includes two suction cups 912, two transverse slide rails 916 and two longitudinal slide rails 915. The two transverse slide rails 916 are both located on the right side of the identification shell 91. The two longitudinal slide rails 915 are slidably connected to the two transverse slide rails 916 through transverse sliders 917 respectively. An adsorption rod 913 is fixedly provided on the upper end of the two suction cups 912 respectively.

[0071] The two adsorption rods 913 are slidably connected to the two longitudinal slide rails 915 through the longitudinal sliders 914 respectively. An adsorption motor 919 is fixedly provided on the upper ends of the two adsorption rods 913 respectively. The upper ends of the two longitudinal slide rails 915 are connected through a connecting piece 920.

[0072] The replacement component can replace the reagent card 925, preventing the staff from manually touching the contaminated reagent card 925, which is safer and more convenient. The waste liquid bottle 6 collects waste liquid, making the cleaning of the sampling cup 2 more automated and convenient.

[0073] A method for using a bioaerosol detection and sampling machine comprises the following steps:

[0074] S1: sampling and titration operation;

[0075] The first peristaltic pump 3 is started to move the liquid medium in the liquid storage bottle 4 to the sampling cup 2, and the sampling fan 1 is started to collect the aerosol particles into the sampling cup 2. After the aerosol particles are mixed with the liquid medium, the sample passes through the second peristaltic pump 5, the first three-way valve 7 and the second three-way valve 8 in sequence, and falls into the detection hole 924 through the burette;

[0076] S2: identification operation;

[0077] The controller 926 starts the driving motor 99, the driving shaft 910 drives the card changing plate 98 to rotate, the driving rod 911 rotates to each rotating hole in turn, drives the rotating plate 96 to rotate, the receiving plate 94 moves upward to contact with the lower end of the detection plate 92, the sample drips onto the reagent card 925 through the detection hole 924, the instrument plate 95 moves upward, and the photoelectric probe 918 performs an identification operation on the reagent card 925 through the detection hole 928;

[0078] After the test, if the result is positive, the sample retention operation is performed, and the controller 926 starts the second peristaltic pump 5 to transport the excess sample in the sampling cup 2 to the sample retention tube 10 through the first three-way valve 7 and the second three-way valve 8 in sequence;

[0079] If the result is negative, the controller 926 starts the second peristaltic pump 5 to transfer the excess sample in the sampling cup 2 to the waste liquid bottle 6 through the first three-way valve 7;

[0080] S3: cleaning operation;

[0081] The controller 926 performs a cleaning procedure to deliver the liquid medium in the liquid storage bottle 4 to the sampling cup 2 for cleaning. The cleaned liquid flows into the waste liquid bottle 6 through the second peristaltic pump 5 and the first three-way valve 7 to perform the next round of sampling operation.

[0082] The controller 926 is started, the longitudinal slider 914 drives the two adsorption rods 913 to move upward, the transverse slider 917 drives the two longitudinal slide rails 915 to move to the left, the two suction cups 912 move to the reagent card 925, the longitudinal slider 914 drives the two adsorption rods 913 to move downward, the adsorption motor 919 is started, the suction cup 912 adsorbs the reagent card 925, the transverse slider 917 drives the two longitudinal slide rails 915 to move to the right, the used reagent card 925 is moved to the outside, and then the new reagent card 925 is adsorbed on the suction cup 912, and the new reagent card 925 is moved to the receiving tray 94.

[0083] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A bioaerosol detection and sampling machine, characterized by: The invention comprises a sampling cup (2), a liquid storage bottle (4), a waste liquid bottle (6), an identification component (9) and a sample retention tube (10); a sampling fan (1) is fixedly arranged at the upper end of the sampling cup (2); the left end of the sampling cup (2) is connected to the liquid storage bottle (4); and a first three-way valve (7) is fixedly arranged at the lower end of the sampling cup (2); A second three-way valve (8) is fixedly arranged at the right end of the first three-way valve (7), the left end of the first three-way valve (7) is connected to the waste liquid bottle (6), a burette is fixedly arranged at the lower end of the second three-way valve (8), the identification component (9) is located at the lower end of the burette, and the right end of the second three-way valve (8) is connected to the sample retention tube (10); The identification component (9) comprises an identification housing (91), a detection disk (92), a receiving disk (94), an instrument disk (95), a rotating shaft (93) and a replacement component, wherein the detection disk (92) is located inside the identification housing (91), the detection disk (92), the receiving disk (94) and the instrument disk (95) are all sleeved on the rotating shaft (93), the detection disk (92) is located above the receiving disk (94), the receiving disk (94) is located above the instrument disk (95), and the detection disk (92) is fixedly connected to the rotating shaft (93); Three movable slide rails (922) are evenly fixedly arranged at the lower end of the detection plate (92), three receiving slide blocks (923) are evenly fixedly arranged at the circumference of the receiving plate (94), and three instrument slide blocks (921) are evenly fixedly arranged at the circumference of the instrument plate (95), and the three receiving slide blocks (923) and the three instrument slide blocks (921) are respectively slidably connected to the three movable slide rails (922); The receiving tray (94) is provided with a reagent card (925), the instrument tray (95) is evenly provided with six photoelectric probes (918), the detection tray (92) is provided with six detection holes (924), the receiving tray (94) is provided with six detection holes (928), and the six detection holes (924), the six detection holes (928) and the six photoelectric probes (918) are all aligned; The rotating shaft (93) is provided with a slide groove, the receiving plate (94) and the instrument plate (95) are slidably connected in the slide groove, the three receiving slide blocks (923) and the three instrument slide blocks (921) are all provided with a moving motor, and a controller (926) is fixedly provided inside the identification housing (91); The lower end of the rotating shaft (93) is rotatably connected to the inner bottom of the identification housing (91) through a rotating fixing member (97); a rotating disk (96) is fixedly sleeved on the rotating shaft (93); a driving shaft (910) is arranged inside the identification housing (91); a driving motor (99) is fixedly arranged at the lower end of the driving shaft (910); and a chuck change disk (98) is sleeved on the driving shaft (910); A driving rod (911) is fixedly provided at the lower end of the card changing plate (98), the card changing plate (98) cooperates with the rotating plate (96), the driving rod (911) cooperates with the rotating hole provided on the rotating plate (96), and the driving motor (99) is fixedly connected to the inner bottom of the identification shell (91) through a motor fixing member (927).

2. The bioaerosol detection and sampling integrated machine according to claim 1, characterized in that: A first peristaltic pump (3) is fixedly arranged between the sampling cup (2) and the liquid storage bottle (4), and a second peristaltic pump (5) is fixedly arranged between the sampling cup (2) and the first three-way valve (7).

3. The bioaerosol detection and sampling integrated machine according to claim 1, characterized in that: The upper surface of the detection disk (92) and the upper surface of the identification housing (91) are located on the same horizontal plane.

4. The bioaerosol detection and sampling integrated machine according to claim 1, characterized in that: The detection plate (92) is a hexagonal structure, the receiving plate (94) and the instrument plate (95) are both circular structures, and a movable hole is provided on the reagent card (925), and the width of the movable hole is greater than the diameter of the rotating shaft (93).

5. The bioaerosol detection and sampling integrated machine according to claim 1, characterized in that: The right side of the identification shell (91) is provided with an adsorption hole, and the replacement component comprises two suction cups (912), two transverse slide rails (916) and two longitudinal slide rails (915). The two transverse slide rails (916) are both located on the right side of the identification shell (91), and the two longitudinal slide rails (915) are respectively slidably connected to the two transverse slide rails (916) through transverse sliders (917), and an adsorption rod (913) is fixedly provided on the upper end of each of the two suction cups (912).

6. The bioaerosol detection and sampling integrated machine according to claim 5, characterized in that: The two adsorption rods (913) are slidably connected to the two longitudinal slide rails (915) through longitudinal sliders (914), respectively; an adsorption motor (919) is fixedly provided at the upper end of the two adsorption rods (913), and the upper ends of the two longitudinal slide rails (915) are connected through a connecting piece (920).

7. A method for using a bioaerosol detection and sampling integrated machine, applied to the bioaerosol detection and sampling integrated machine as claimed in any one of claims 1 to 6, characterized in that: Includes steps: S1: sampling and titration operation; The first peristaltic pump (3) is started to move the liquid medium in the liquid storage bottle (4) into the sampling cup (2), the sampling fan (1) is started to collect the aerosol particles into the sampling cup (2), and after the aerosol particles are mixed with the liquid medium, the sample passes through the second peristaltic pump (5), the first three-way valve (7) and the second three-way valve (8) in sequence, and falls into the detection hole (924) through the burette; S2: identification operation; The controller (926) starts the driving motor (99), the driving shaft (910) drives the card changing disk (98) to rotate, the driving rod (911) rotates to each rotating hole in turn, drives the rotating disk (96) to rotate, the receiving disk (94) moves upward to contact with the lower end of the detection disk (92), the sample drips onto the reagent card (925) through the detection hole (924), the instrument disk (95) moves upward, and the photoelectric probe (918) performs an identification operation on the reagent card (925) through the detection hole (928); After the test, if the result is positive, the sample retention operation is performed, and the controller (926) starts the second peristaltic pump (5) to transport the excess sample in the sampling cup (2) to the sample retention tube (10) through the first three-way valve (7) and the second three-way valve (8) in sequence; If the result is negative, the controller (926) starts the second peristaltic pump (5) to transfer the excess sample in the sampling cup (2) to the waste liquid bottle (6) through the first three-way valve (7); S3: cleaning operation; The controller (926) performs a cleaning procedure to transfer the liquid medium in the liquid storage bottle (4) to the sampling cup (2) for cleaning. The cleaned liquid flows into the waste liquid bottle (6) through the second peristaltic pump (5) and the first three-way valve (7) to perform the next round of sampling operation. The controller (926) is started, the longitudinal slider (914) drives the two adsorption rods (913) to move upward, the transverse slider (917) drives the two longitudinal slide rails (915) to move leftward, the two suction cups (912) move to the reagent card (925), the longitudinal slider (914) drives the two adsorption rods (913) to move downward, the adsorption motor (919) is started, the suction cup (912) adsorbs the reagent card (925), the transverse slider (917) drives the two longitudinal slide rails (915) to move rightward, the used reagent card (925) is moved to the outside, and then a new reagent card (925) is adsorbed on the suction cup (912), and the new reagent card (925) is moved to the receiving tray (94).

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