Reagent filling equipment

Abstract

The present invention discloses a reagent perfusion device. The reagent perfusion device includes a platform, a storage container, a loading device, a reagent network and a blocking device. The storage container is used to store reagents, the loading device is used to clamp a chip, the chip is provided with a reagent channel, the reagent network includes a pump and a pipeline connected to the pump, the pump is used to pump the reagent in the storage container into the reagent channel through the pipeline, and the blocking device is used to block the reagent channel after the reagent is pumped into the reagent channel; wherein the storage container, the loading device, the reagent network and the blocking device are all arranged on the platform. In this way, the reagent perfusion device is used to facilitate the chip perfusion of reagents without manual participation, thereby improving the efficiency of the chip perfusion of reagents, and the pump can realize the quantitative perfusion of reagents, and the reagents are transported from the storage container to the chip through the pipeline, so that the reagents are not easy to overflow during the transfer process.

Description

Technical Field

[0001] The present invention relates to the technical field of biochips, and in particular to a reagent perfusion device. Background Art

[0002] In the process of molecular biology research and application, the analyzer can be used to perform electrophoresis analysis on samples containing biological substances such as DNA, RNA or protein through electrophoresis technology. For nucleic acid samples containing DNA or RNA, when the chip is in the analysis station, the electrophoresis mechanism of the analyzer applies voltage to the nucleic acid sample in the chip, and the nucleic acid sample moves under the action of the electric field force. Since there are nucleic acid fragments of different lengths in the nucleic acid sample, differential separation of nucleic acid fragments of different lengths is achieved to form multiple bands. After the nucleic acid sample completes the electrophoresis separation, the imaging mechanism of the analyzer takes a photo of the nucleic acid sample and analyzes the photo to obtain information such as the length, concentration and integrity of the nucleic acid fragments corresponding to each band of the nucleic acid sample.

[0003] Before placing the chip at the analysis station, gel and buffer need to be injected into the reagent channel of the chip. During electrophoresis, the buffer plays a role in maintaining the degree of dissociation, and the nucleic acid sample is electrophoretically separated in the gel. In the related art, manual perfusion of gel and buffer is adopted. However, manual perfusion has low efficiency, reagents are not easy to quantify, and reagents are easy to overflow during the transfer process. Summary of the invention

[0004] The invention provides a reagent perfusion device.

[0005] The reagent perfusion equipment of the embodiment of the present application includes a platform, a storage container, a loading device, a reagent network and a blocking device. The storage container is used to store reagents, the loading device is used to clamp the chip, the chip is provided with a reagent channel, the reagent network includes a pump and a pipeline connected to the pump, the pump is used to pump the reagent in the storage container into the reagent channel through the pipeline, and the blocking device is used to block the reagent channel after the reagent is pumped into the reagent channel; wherein the storage container, the loading device, the reagent network and the blocking device are all arranged on the platform.

[0006] In this way, the loading device can keep the position of the chip stable during the reagent perfusion process, the reagent in the storage container can be pumped into the reagent channel of the chip through the reagent network, and the blocking device blocks the reagent channel after the reagent enters the reagent channel to prevent leakage of the reagent. The reagent perfusion equipment is used to facilitate the perfusion of the chip with reagents without manual intervention, thereby improving the efficiency of perfusing the chip with reagents, and the pump can realize quantitative perfusion of the reagent, and transport the reagent from the storage container to the chip through the pipeline, so that the reagent is not easy to overflow during the transfer process.

[0007] In certain embodiments, the blocking device includes a heating rod and a hot melt head, one end of the hot melt head is connected to the heating rod, and the other end is used to abut against the chip to block the reagent channel by hot melting.

[0008] In some embodiments, the hot melt head includes a hot melt block and a clamping block arranged on one side in the height direction of the hot melt block, the end face of the hot melt block and the end face of the clamping block are both facing the loading device, the end face of the clamping block is a plane, and the end face of the hot melt block is a concave surface that is concave away from the loading device.

[0009] In certain embodiments, the chip is provided with a liquid inlet and a connecting channel, the liquid inlet is connected to the reagent channel through the connecting channel, the reagent perfusion device includes a pressing block connected to the pipeline, the pressing block includes an interface connected to the liquid inlet, and the pump is used to drive the reagent in the storage container to pass through the interface, the liquid inlet and the connecting channel in sequence and enter the reagent channel.

[0010] In certain embodiments, the reagents include gel and cleaning fluid, the reagent channel is provided with a plurality of first channels connected one-to-one with the reagent channels, the liquid inlet hole includes a plurality of first holes connected one-to-one with the first channels, the interface includes a first interface connected with the first hole, and the pump is used to drive the gel or the cleaning fluid to pass through the first interface, the first hole and the first channel in sequence and enter the corresponding reagent channel.

[0011] In some embodiments, the interface includes a second interface connected to the pipeline, and the second interface is connected to the first interface in a one-to-one correspondence.

[0012] In certain embodiments, a plurality of the second interfaces are arranged in two rows, each row of the second interfaces is arranged along a first direction, and along the first direction, the two rows of the second interfaces are staggered, and the first direction is the same as the arrangement direction of the reagent channels.

[0013] In certain embodiments, the pressing block comprises an intersecting first surface and a second surface, wherein an angle formed between the first surface and the second surface in a direction toward the loading device is an acute angle; wherein one row of the second interfaces is arranged on the first surface, and another row of the second interfaces is arranged on the second surface.

[0014] In certain embodiments, the reagents include a buffer solution and a cleaning solution, a plurality of reagent channels are provided, the connecting channel includes a second channel connected to the reagent channel, the liquid inlet hole includes a second hole connected to the second channel, the interface includes a third interface connected to the second hole, and the pump is used to drive the buffer solution or the cleaning solution to pass through the third interface, the second hole, and the second channel in sequence and enter the reagent channel.

[0015] In certain embodiments, there are multiple second channels, and there are two second holes. The first second channel connects one end of the first reagent channel and one of the second holes, the last second channel connects one end of the last reagent channel and another second hole, and the remaining second channels connect two adjacent reagent channels.

[0016] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0018] Figure 1 is a schematic structural diagram of a chip according to an embodiment of the present invention;

[0019] Figure 2 is a schematic structural diagram of a reagent perfusion device according to one embodiment of the present invention;

[0020] Figure 3 is a schematic structural diagram of a reagent perfusion device according to one embodiment of the present invention;

[0021] Figure 4 is a schematic structural diagram of a reagent perfusion device according to one embodiment of the present invention;

[0022] Figure 5 is a schematic structural diagram of a reagent perfusion device according to one embodiment of the present invention;

[0023] Figure 6 is a schematic cross-sectional view of a partial structure of a pressing block according to an embodiment of the present invention;

[0024] Figure 7 It is a partial structural schematic diagram of a pressing block according to an embodiment of the present invention;

[0025] Figure 8 is a schematic structural diagram of a reagent perfusion device according to one embodiment of the present invention;

[0026] Fig. 9is a schematic structural diagram of a reagent perfusion device according to another embodiment of the present invention;

[0027] Fig.10 is a schematic structural diagram of a reagent perfusion device according to another embodiment of the present invention;

[0028] Fig.11 yes Fig.10 Schematic cross-sectional view along the AA direction;

[0029] Fig.12 is a schematic structural diagram of a blocking device according to an embodiment of the present invention;

[0030] Fig.13 is a schematic structural diagram of a reagent perfusion device according to another embodiment of the present invention;

[0031] Fig.14 is a schematic structural diagram of a reagent perfusion device according to another embodiment of the present invention;

[0032] Fig.15 is a schematic structural diagram of a reagent perfusion device according to another embodiment of the present invention;

[0033] Fig.16 is a schematic cross-sectional view of a storage container according to an embodiment of the present invention;

[0034] Fig.17 yes Figure 5 Schematic cross-sectional view along direction BB.

[0035] Explanation of reference numerals: 100, reagent filling device; 10, platform; 11, supporting foot; 12, shell; 13, hot air blower; 14, lighting device; 20, storage container; 21, heating device; 22, heat preservation device; 30, loading device; 31, loading space; 40, reagent network; 41, pump; 50, blocking device; 51, heating rod; 52, hot melt head; 53, hot melt block; 54, pressing block; 55, adapter; 60, first driving member; 61, motor; 62, output shaft; 63, first detection device; 64, first stopper; 65, second driving member; 66, second detection device; 67, second stopper; 70, heat dissipation device; 71 , fan; 72, housing; 73, air outlet; 80, pressure block; 81, interface; 811, first interface; 812, second interface; 82, first surface; 83, second surface; 813, third interface; 84, third surface; 85, fourth surface; 86, main body; 87, connecting part; 88, guide groove; 90, rotary valve; 91, common port; 92, first port; 200, chip; 201, substrate; 202, cover plate; 210, reagent channel; 220, liquid inlet hole; 221, first hole; 222, second hole; 230, connecting channel; 231, first channel; 232, second channel; 240, sealing ring; 241, through hole. DETAILED DESCRIPTION

[0036] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0037] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0038] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0040] The disclosure below provides many different embodiments or examples to realize different structures of the present invention. In order to simplify the disclosure of the present invention, the parts and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the present invention. In addition, the present invention can repeat reference numbers and / or reference letters in different examples, and this repetition is for the purpose of simplicity and clarity, which itself does not indicate the relationship between the various embodiments and / or settings discussed. In addition, the present invention provides various specific examples of processes and materials, but those of ordinary skill in the art can be aware of the application of other processes and / or the use of other materials.

[0041] See also Figure 1-Figure 3 The reagent perfusion device 100 of the embodiment of the present application includes a platform 10, a storage container 20, a loading device 30, a reagent network 40 and a blocking device 50. The storage container 20 is used to store reagents, the loading device 30 is used to clamp the chip 200, and the chip 200 is provided with a reagent channel 210. The reagent network 40 includes a pump 41 and a pipeline connected to the pump 41. The pump 41 is used to pump the reagent in the storage container 20 into the reagent channel 210 through the pipeline. The blocking device 50 is used to block the reagent channel 210 after the reagent is pumped into the reagent channel 210; wherein the storage container 20, the loading device 30, the reagent network 40 and the blocking device 50 are all arranged on the platform 10.

[0042] In this way, the loading device 30 can keep the position of the chip 200 stable during the reagent perfusion process, and the reagent in the storage container 20 can be pumped into the reagent channel 210 of the chip 200 through the reagent network 40. The blocking device 50 blocks the reagent channel 210 after the reagent enters the reagent channel 210 to prevent leakage of the reagent. The reagent perfusion equipment 100 is used to facilitate the perfusion of the reagent into the chip 200 without manual intervention, thereby improving the efficiency of perfusing the reagent into the chip 200. The pump 41 can realize quantitative perfusion of the reagent and transport the reagent from the storage container 20 to the chip 200 through the pipeline, so that the reagent is not easy to overflow during the transfer process.

[0043] Specifically, the reagent perfusion device 100 is used to inject reagents into the chip 200. The chip 200 can be used to load samples containing biological substances such as DNA, RNA or protein, and the sample in the chip 200 is subjected to electrophoresis analysis by using an analyzer. The analyzer may include a loading mechanism, a fixing mechanism, an electrophoresis mechanism and an imaging mechanism. The loading mechanism loads the sample into the reagent channel 210 of the chip 200. The chip 200 after loading can be directly placed in the analysis station of the fixing mechanism, or can be placed at the entrance of the analyzer. The chip 200 located at the entrance is moved to the analysis station of the fixing mechanism by a moving mechanism such as a mechanical arm or a conveyor belt. When the chip 200 is at the analysis station, the fixing mechanism fixes the chip 200 to prevent the chip 200 from moving when the chip 200 is subsequently subjected to electrophoretic separation and photographic imaging. After the fixing mechanism completes the fixing of the chip 200, the electrophoresis mechanism performs electrophoretic separation on the sample in the chip 200. After the electrophoresis separation is completed, the imaging mechanism takes a photo of the sample and then analyzes the photo to obtain information such as the fragment length, concentration and integrity of the sample. After the analysis is completed, the fixing mechanism releases the chip 200 and does not fix the chip 200, so that the chip 200 can be moved and recovered. After the analysis and recovery of one chip 200 is completed, the above operation is performed on the next chip 200 until the analysis and recovery of all chips 200 are completed.

[0044] The platform 10 may be a flat plate, the surface of which is in the same horizontal plane. The storage container 20, the loading device 30 and the pump 41 may be fixed on the upper surface of the platform 10 by fasteners. The blocking device 50 may be partially fixed on the lower surface of the platform 10, or partially penetrate the platform 10. The reagent network 40 and the blocking device 50 may be arranged on the same side of the loading device 30, or may be arranged on both sides of the loading device 30 respectively. Since the loading device 30 clamps the chip 200, that is to say, the reagent network 40 and the blocking device 50 may be arranged on the same side of the chip 200, or may be arranged on both sides of the chip 200 respectively. The lower surface of the platform 10 may be provided with a plurality of support legs 11, which provide support for the platform 10.

[0045] The storage container 20 can be a reagent bottle, and the storage container 20 is connected to the pump 41 through a pipeline. Reagents include but are not limited to cleaning solutions, gels, and buffers. The number of storage containers 20 can be multiple, and multiple storage containers 20 can store multiple different reagents. The cross-sectional shape of the pipeline can be circular, and the length and diameter of the pipeline can be set according to actual needs. The pump 41 can be a quantitative pump, such as a piston pump, a vane pump, or a gear pump, etc. The pump 41 can provide positive pressure or negative pressure.

[0046] See also Figure 2 and Figure 4 , the loading device 30 can fix the chip 200, and provide support and protection for the chip 200. The loading device 30 can be formed with a vertical loading space 31, and the chip 200 is vertically located in the loading space 31, that is, the thickness direction of the chip 200 is the horizontal direction. When the chip 200 is provided with a single reagent channel 210, the length of the reagent channel 210 can extend along the length direction of the chip 200; when the chip 200 is provided with multiple reagent channels 210, such as 4, 8, 12 or 16, the length of the reagent channel 210 can extend along the width direction of the chip 200, and the multiple reagent channels 210 can be arranged along the length direction of the chip 200. The material of the chip 200 includes inorganic insulating materials, organic insulating materials, polymer insulating materials, composite materials, polymers or combined materials, preferably polypropylene materials, which have good light transmittance, and the surface of the polypropylene material will not dissociate ions in the water environment. In the case of no surface treatment, the electroosmotic effect can be avoided, thereby avoiding affecting the electrophoretic separation process of the sample. The chip 200 is made by one or more combination of laser engraving technology, micro-electromechanical processing technology, screen printing technology, 3D printing technology, and photolithography technology. The reagent channel 210 is formed on the chip 200 by engraving or injection molding technology. The reagent channel 210 can have a certain length to provide a sufficient moving distance for the sample to allow the sample to be differentially separated. The cross-sectional shape of the reagent channel 210 can be rectangular or arc-shaped.

[0047] The blocking device 50 can block the reagent channel 210 by hot melting or by setting a plug. After the reagent channel 210 is blocked, the two sides of the reagent channel 210 can be cut to cut off the excess part of the chip 200 to make a chip 200 for electrophoresis.

[0048] See also Figure 1 and Figure 4In some embodiments, the chip 200 is provided with a liquid inlet 220 and a connecting channel 230, the liquid inlet 220 is connected to the reagent channel 210 through the connecting channel 230, the reagent perfusion device 100 includes a pressing block 80 connected to the pipeline, the pressing block 80 includes an interface 81 connected to the liquid inlet 220, and the pump 41 is used to drive the reagent in the storage container 20 to pass through the interface 81, the liquid inlet 220 and the connecting channel 230 in sequence and enter the reagent channel 210.

[0049] See also Figure 1 and Figure 5 The chip 200 includes a substrate 201 and a cover plate 202 disposed on the substrate 201. The reagent channel 210 and the connection channel 230 are formed on the substrate 201. The liquid inlet 220 is a hole formed by extending inward from the surface of the cover plate 202 of the chip 200. The cover plate 202 can provide protection for the substrate 201. After the analysis process is completed, the sample in the reagent channel 210 may leak. The cover plate 202 can prevent the sample from overflowing the chip 200. The cover plate 202 can be made of a transparent material, which can ensure that when the imaging mechanism takes pictures of the sample, a clear photo can be obtained smoothly, and the photo will not be blurred due to the cover plate 202.

[0050] See also Figure 1 , Figure 4 and Figure 6 In some embodiments, the reagent includes gel and cleaning fluid, a plurality of reagent channels 210 are provided, the connecting channel 230 includes a plurality of first channels 231 connected to the reagent channels 210 in a one-to-one correspondence, the liquid inlet hole 220 includes a plurality of first holes 221 connected to the first channels 231 in a one-to-one correspondence, the interface 81 includes a first interface 811 connected to the first hole 221, and the pump 41 is used to drive the gel or the cleaning fluid to pass through the first interface 811, the first hole 221 and the first channel 231 in sequence and enter the corresponding reagent channel 210.

[0051] The number of the storage containers 20 may be two, and the two storage containers 20 contain gel and cleaning solution respectively. The cleaning solution can be used to clean the pipeline before the chip 200 is filled with gel.

[0052] Different types of gels can be used, including but not limited to agarose, polyacrylamide and starch. The gel is a porous structure and can be used as a sieving medium to separate proteins, nucleic acids and particles. Polyacrylamide gel can be used to separate nucleic acids, including small fragments of nucleic acids (e.g., about 5bp-500bp). Agarose gel can be used to separate proteins, including proteins above about 200kDa. Agarose gel can be used to separate nucleic acids, including nucleic acids from about 50bp in size to and including nucleic acids of several Mb in size. When pouring agarose gel, the gel needs to be heated to prevent the gel from solidifying so as not to affect the pouring efficiency. When the chip 200 is poured with agarose gel by the reagent pouring device 100, the difficulty of artificially pouring gel can be reduced due to the high temperature. When pouring polyacrylamide gel, there is no need to heat the gel. When the chip 200 is poured with polyacrylamide gel by the reagent pouring device 100, the inconsistent techniques of artificial pouring and the long pouring time can be prevented, resulting in low pouring efficiency.

[0053] Different gel compositions can be used. The porosity of the gel can be affected by the composition of the gel. Gels of different porosities can provide improved resolution for samples of a specific size range. In some cases, an agarose gel can include between about 0.7% and about 2% agarose. In some cases, an agarose gel can include between about 0.7% and about 3% agarose. In some cases, a polyacrylamide gel can include between about 6% and about 15% polyacrylamide.

[0054] The gel may contain a fluorescent dye, and through the transparent bottom plate, the gel with the fluorescent dye can excite fluorescence in real time, and through the transparent cover plate 202, the emission filter can be used to observe and record the entire sample separation process in real time, and photos can also be taken at the same time to obtain results in real time and archive them. The cleaning solution includes but is not limited to one or more of water, organic solvents, and inorganic solvents.

[0055] Different reagent channels 210 can be loaded with different types or the same type of gel. For example, some reagent channels 210 are loaded with agarose gel while other reagent channels 210 are loaded with polyacrylamide gel.

[0056] Different reagent channels 210 may be loaded with gels of the same or different densities or porosities. For example, some reagent channels 210 may be loaded with 6% polyacrylamide gels while other reagent channels 210 may be loaded with 12% polyacrylamide gels.

[0057] Different reagent channels 210 can be used to separate the same type of material at the same time, can be used to separate different types of materials at the same time, and can be used to separate materials of different size ranges. Enhanced resolution within each subset of the size range of the sample material can be provided by using different gel compositions customized for each size range. In some cases, molecules that differ by at least 1, 2, 3, 4, or 5 orders of magnitude in size or molecular weight can be separated or resolved within a gel. For example, nucleic acid molecules between 10 bases and 10kb bases can be separated or resolved within a gel. In another example, the sample can include sample molecules of three size ranges, and a portion of the sample can be loaded into three reagent channels 210; the first size range is well resolved in the first reagent channel 210, the second size range is well resolved in the second reagent channel 210, and the third size range is well resolved in the third reagent channel 210.

[0058] The first channel 231 may extend along a straight path from one end of the reagent channel 210 to the corresponding first hole 221 to shorten the path of the gel from the first hole 221 through the first channel 231 into the reagent channel 210 , thereby improving the perfusion efficiency of the gel.

[0059] See also Figure 1 and Figure 4 In some embodiments, the reagent channel 210 extends from bottom to top, the first hole 221 is located on one side of the lower end of the reagent channel 210, and the pump 41 is used to drive the gel or cleaning solution through the first hole 221 from bottom to top into the corresponding reagent channel 210.

[0060] In this way, the gel can be gathered in the lower part of the reagent channel 210 to prevent the gel from remaining in the upper part of the reagent channel 210. The reagent channel 210 can be the channel of the electrophoresis chip 200, or it can be the channel of the simulation chip 200. The electrophoresis chip 200 is used to perform electrophoresis to separate samples. The simulation chip 200 is used to connect to the pipeline before the gel is injected into the electrophoresis chip 200, so that the cleaning solution flows into the simulation chip 200 through the pipeline, and the pump 41 and the pipeline are cleaned. The simulation chip 200 is discarded after the cleaning solution cleans the pipeline. The pump 41 is used to drive the gel into the reagent channel 210 of the electrophoresis chip 200, and to drive the cleaning solution into the reagent channel 210 of the simulation chip 200. The gel is in liquid state when entering the reagent channel 210. After the perfusion is completed, it can be naturally left to stand to solidify, or it can be transferred to a curing device to accelerate curing to solidify.

[0061] See also Figure 6 In some embodiments, the interface 81 includes a second interface 812 connected to the pipeline, and the second interface 812 is connected to the first interface 811 in a one-to-one correspondence.

[0062] The first interface 811 may be located on the surface of the pressing block 80 facing the chip 200, the second interface 812 may be located on the surface of the pressing block 80 facing the storage container 20, and the surface where the first interface 811 is located may be in contact with the surface where the first hole 221 is located, so that the gel or cleaning solution in the storage container 20 flows into the reagent channel 210 through the second interface 812 and the first interface 811. Multiple second interfaces 812 may be arranged in one row or in multiple rows.

[0063] See also Figure 1 and Figure 7 In some embodiments, the plurality of second interfaces 812 are arranged in two rows, each row of second interfaces 812 is arranged along a first direction X, and along the first direction X, the two rows of second interfaces 812 are staggered, and the first direction X is the same as the arrangement direction of the reagent channel 210 .

[0064] In this way, the distance between two adjacent second interfaces 812 in each row of second interfaces 812 can be increased, which facilitates the installation and removal of the pipeline and the second interfaces 812. The two rows of second interfaces 812 can be located on the same surface of the pressing block 80, or on different surfaces of the pressing block 80. The number of the two rows of second interfaces 812 can be the same or different.

[0065] See also Figure 5-Figure 7 In some embodiments, the pressing block 80 includes an intersecting first surface 82 and a second surface 83, and in the direction of the first surface 82 and the second surface 83 toward the loading device 30, the angle formed between the first surface 82 and the second surface 83 is an acute angle; one row of second interfaces 812 is arranged on the first surface 82, and another row of second interfaces 812 is arranged on the second surface 83.

[0066] Since the second interface 812 on the first surface 82 extends perpendicular to the first surface 82, and the second interface 812 on the second surface 83 extends perpendicular to the second surface 83, in the direction in which the first surface 82 and the second surface 83 are away from the loading device 30, the angle formed between the central axis of the second interface 812 on the first surface 82 and the central axis of the second interface 812 on the second surface 83 is an obtuse angle, which can reduce the interference between the two rows of second interfaces 812, increase the connection space between the second interface 812 and the pipeline, and facilitate the installation and disassembly of the pipeline and the second interface 812.

[0067] The angle formed between the first surface 82 and the second surface 83 can be 30°, 50°, 70° or other angles, and the second interface 812 can be formed by the first surface 82 and the second surface 83 extending inwardly along the surface perpendicular to itself. In one embodiment, the number of reagent channels 210 is 16, and the number of first channels 231, first holes 221, first interfaces 811, and second interfaces 812 are all 16, of which 8 second interfaces 812 are located on the first surface 82 of the pressing block 80, and the other 8 second interfaces 812 are located on the second surface 83 of the pressing block 80.

[0068] See also Figure 5 and Figure 7 In some embodiments, the reagent network 40 further includes a rotary valve 90 disposed on the platform 10, the rotary valve 90 includes a common port 91 and a plurality of first ports 92, the common port 91 is connected to the storage container 20 through a pipeline, the first ports 92 are connected to the second interface 812 in a one-to-one correspondence, and the common port 91 selectively connects to one of the first ports 92. In this way, different gels can be poured into different reagent channels 210. The pipeline can be connected to the first port 92 and the second interface 812 through a connector.

[0069] The rotary valve 90 is located between the pressing block 80 and the storage container 20, and is arranged on the upper surface of the platform 10. The number of common ports 91 is one, and the number of first ports 92 is consistent with the number of reagent channels 210. The shapes of the common port 91 and the first port 92 can be regular shapes such as circular and polygonal, or irregular shapes. In the embodiment of the present application, in order to facilitate the formation, manufacture and / or connection of the common port 91 and the first port 92 with common pipelines, the common port 91 and the first port 92 are circular. The common port 91 is located in the middle position of the rotary valve 90, and the first port 92 is arranged at intervals along the circumference of the rotary valve 90.

[0070] See also Figure 8 In some embodiments, the reagent perfusion device 100 includes a first driving member 60 disposed on the platform 10, and the first driving member 60 is used to drive the pressing block 80 to move relative to the chip 200 so that the pressing block 80 is pressed against the chip 200. The first driving member 60 can provide driving force for the movement of the pressing block 80 through a motor 61, hydraulic pressure, an air pump, etc.

[0071] See also Figure 5 and Figure 8In some embodiments, the first driving member 60 is arranged on the lower surface of the platform 10, the pressing block 80 includes a main body 86 and a connecting portion 87, the main body 86 is connected to the connecting portion 87, a guide groove 88 is provided on the platform 10 and penetrates the platform 10 along the thickness direction of the platform 10, the connecting portion 87 is penetrated in the guide groove 88, the connecting portion 87 is connected to the first driving member 60, and the first driving member 60 is used to drive the connecting portion 87 to drive the main body 86 to move toward the loading device 30.

[0072] The first driving member 60 is arranged on the lower surface of the platform 10 to save space on the upper surface of the platform 10 and improve the space utilization rate of the platform 10. The main body 86 is mechanically connected to the connecting part 87, the interface 81 is arranged on the main body 86, and the length direction of the guide groove 88 is the thickness direction of the chip 200. The first driving member 60 can be a screw motor 61, the connecting part 87 is slidably connected to the output shaft 62 of the motor 61, the axial direction of the output shaft 62 is consistent with the thickness direction of the chip 200, and the rotation of the motor 61 drives the connecting part 87 to move relative to the axial direction of the output shaft 62, thereby driving the main body 86 to move closer to or away from the loading device 30, so that the interface 81 is connected or disconnected with the liquid inlet 220. The first driving member 60 can also be connected to the connecting part 87 through a transmission device such as a gear, a rack, a conveyor belt, a chain, etc. and output torque.

[0073] See also Figure 8 The lower surface of the platform 10 may be provided with a first detection device 63, which includes a first stopper 64. When the pressing block 80 is pressed against the chip 200, the connecting portion 87 contacts the first stopper 64, the first detection device 63 sends a signal, and the first driving member 60 stops working. In some embodiments, the first detection device 63 may be a photoelectric sensor.

[0074] See also Fig. 9 In some embodiments, the first driving member 60 , the connecting portion 87 and the first detecting device 63 are disposed on the upper surface of the platform 10 .

[0075] See also Figure 1 , Fig.10 and Fig.11 In some embodiments, the reagent includes a buffer solution and a cleaning solution, and the reagent channel 210 is provided with multiple channels. The connecting channel 230 includes a second channel 232 connected to the reagent channel 210, the liquid inlet hole 220 includes a second hole 222 connected to the second channel 232, and the interface 81 includes a third interface 813 connected to the second hole 222. The pump 41 is used to drive the buffer solution or the cleaning solution to pass through the third interface 813, the second hole 222, and the second channel 232 in sequence and enter the reagent channel 210.

[0076] The number of storage containers 20 can be two, and the two storage containers 20 contain a buffer solution and a cleaning solution respectively. The cleaning solution can clean the pipeline before the buffer solution is perfused into the chip 200. The buffer solution can make the solution have a certain conductivity to facilitate the migration of DNA molecules, and can also play a role in maintaining the degree of dissociation, thereby achieving differential separation of sample fragments of different lengths. Sample fragments of different lengths form strips in the reagent channel 210 to complete the electrophoresis separation process.

[0077] The buffer may be a TAE buffer, which is composed of Tris base, acetic acid, and ethylenediaminetetraacetic acid (EDTA). The buffer may also be a MOPS buffer, a TBE buffer, or other buffers corresponding to the sample separation requirements. The buffers used in each reagent channel 210 may be the same or different.

[0078] See also Fig.11 The pressing block 80 includes a third surface 84 and a fourth surface 85 relative to each other, the fourth surface 85 is fitted with the chip 200, the third surface 84 is connected to the pipeline through a joint, and the third interface 813 extends from the third surface 84 to the fourth surface 85. The third interface 813 can be stepped, that is, the size of the third interface 813 close to the third surface 84 can be larger than the size of the third interface 813 close to the fourth surface 85, and the part of the third interface 813 close to the third surface 84 can be a threaded hole, so that the joint can be screwed into the third interface 813 from the third surface 84, thereby fixed on the pressing block 80.

[0079] See also Figure 1 and Fig. 9 In some embodiments, the reagent channel 210 extends from top to bottom, the second hole 222 is located on one side of the high end of the reagent channel 210, and the pump 41 is used to drive the buffer solution or the cleaning solution to enter the reagent channel 210 from top to bottom through the second hole 222.

[0080] In this way, the buffer solution can be gathered in the upper space of the reagent channel 210, which is convenient for separating the gel and the buffer solution. The reagent channel 210 can be the channel of the electrophoresis chip 200, or it can be the channel of the simulation chip 200. The electrophoresis chip 200 is used to perform electrophoresis to separate samples. The simulation chip 200 is used to connect to the pipeline before injecting the buffer solution into the electrophoresis chip 200, so that the cleaning solution flows into the simulation chip 200 through the pipeline, and the pump 41 and the pipeline are cleaned. The simulation chip 200 is discarded after the cleaning solution cleans the pipeline. The pump 41 is used to drive the buffer solution into the reagent channel 210 of the electrophoresis chip 200, and to drive the cleaning solution into the reagent channel 210 of the simulation chip 200.

[0081] See also Figure 1In some embodiments, there are multiple second channels 232 and two second holes 222. The first second channel 232 connects one end of the first reagent channel 210 and one of the second holes 222. The last second channel 232 connects one end of the last reagent channel 210 and another second hole 222. The remaining second channels 232 connect two adjacent reagent channels 210.

[0082] In this way, multiple reagent channels 210 can be connected in series through multiple second channels 232, so that the buffer solution can enter the multiple reagent channels 210 in sequence from one of the second holes 222, and the air in the reagent channel 210 flows out from another second hole 222. After the perfusion is completed, the excess buffer solution flows out from another second hole 222.

[0083] The second channel 232 may extend along a curved path or a zigzag path from one end of the reagent channel 210 to one end of the adjacent reagent channel 210. In one embodiment, the number of the reagent channels 210 is 16, the number of the second channels 232 is 17, the number of the second holes 222 is two, the first second channel 232 connects the first reagent channel 210 and one of the second holes 222, the second second channel 232 connects the first reagent channel 210 and the second reagent channel 210, the third second channel 232 connects the second reagent channel 210 and the third reagent channel 210, and so on, the sixteenth second channel 232 connects the fifteenth reagent channel 210 and the sixteenth reagent channel 210, and the seventeenth second channel 232 connects the sixteenth reagent channel 210 and another second hole 222.

[0084] In one embodiment, the number of reagent channels 210 is 4, the number of second channels 232 is 8, the number of second holes 222 is two, the first second channel 232 connects the first reagent channel 210 and one of the second holes 222, the second second channel 232 connects the first reagent channel 210, the third second channel 232 and the fourth second channel 232 connect the second reagent channel 210, the fifth second channel 232 and the sixth second channel 232 connect the third reagent channel 210, the seventh second channel 232 connects the fourth reagent channel 210, and the eighth second channel 232 connects the fourth reagent channel 210 and another second hole 222. The second second channel 232 and the third second channel 232, the fourth second channel 232 and the fifth second channel 232, and the sixth second channel 232 and the seventh second channel 232 may be connected through grooves or holes to buffer the flow of the buffer solution.

[0085] In one embodiment, the number of reagent channels 210 and second channels 232 is 16, the number of second holes 222 is two, one end of the 16 second channels 232 is connected to the 16 reagent channels 210 one by one, the other end of the 16 second channels 232 is connected through a confluence channel, and the two second holes 222 are respectively located at the two ends of the confluence channel.

[0086] See also Fig.11 and Fig.14 In some embodiments, a sealing ring 240 is disposed between the pressing block 80 and the chip 200 , and the sealing ring 240 seals the gap between the interface 81 and the liquid inlet 220 .

[0087] In this way, the sealing performance between the pressing block 80 and the chip 200 can be improved, and the reagent can be prevented from leaking from the gap between the interface 81 and the liquid inlet 220 , thereby improving the efficiency of perfusing the reagent into the chip 200 .

[0088] Specifically, the sealing ring 240 can be made of rubber material, and can be arranged on the side of the pressing block 80 facing the chip 200, or on the side of the chip 200 facing the pressing block 80. The sealing ring 240 is formed with a through hole 241 along the thickness direction, and the interface 81 is connected to the liquid inlet through the through hole 241.

[0089] In some embodiments, the number of storage containers 20 is three, and the three storage containers 20 store cleaning liquid, gel and buffer respectively. The pressing block 80 includes two main bodies 86 and a connecting part 87, wherein the bottom surface of one main body 86 is connected to the upper surface of the connecting part 87 to connect the storage container 20 storing cleaning liquid or buffer with the second hole 222, and the lower surface of the connecting part 87 extends upward to form an installation space, which penetrates the connecting part 87 along the thickness direction of the connecting part 87, and the other main body 86 is located in the installation space and connected to the connecting part 87 through the side or top surface to connect the storage container 20 storing cleaning liquid or gel with the first hole 221. Two guide grooves 88 are provided on the platform 10, and the length direction of the guide grooves 88 is the same as the thickness direction of the chip 200. The two guide grooves 88 are respectively located on both sides of the length direction of the main body 86, and the length direction of the main body 86 is the same as the arrangement direction of the reagent channel 210. The connection position of the connecting part 87 and the two guide grooves 88 is respectively located on both sides of the length direction of the main body 86. The upper surface of the platform 10 is provided with a first driving member 60, which is connected to the connecting portion 87. The first driving member 60 can be located on the side of one of the guide grooves 88 away from the installation space, or can be located between one of the guide grooves 88 and the installation space. The first driving member 60 drives the connecting portion 87 to slide on the guide groove 88, driving the connecting portion 87 to move toward the loading device 30, so as to drive the two main bodies 86 to move toward the chip 200 at the same time, so that the reagent perfusion device 100 has the function of perfusing gel and buffer at the same time. In other embodiments, the upper surface of the platform 10 extends upward to form a boss, the first driving member 60 is arranged on the boss, and the position where the connecting portion 87 is connected to the first driving member 60 is located between the two main bodies 86.

[0090] See also Figure 1 and Fig.10 In some embodiments, the blocking device 50 includes a heating rod 51 and a hot melt head 52, one end of the hot melt head 52 is connected to the heating rod 51, and the other end is used to abut against the chip 200 to block the reagent channel 210 by hot melting.

[0091] Specifically, the heating rod 51 can heat the hot melt head 52 by resistive heating. The hot melt head 52 can be made of a material with good thermal conductivity such as metal. In this way, the temperature generated by the heating rod 51 can be transferred to the hot melt head 52, so that the hot melt head 52 can hot-melt the connecting channels 230 at both ends of the reagent channel 210.

[0092] The temperature of the hot melt head 52 can be higher than the melting point of the chip so that the chip can be melted by heat. The temperature and time of the hot melt head 52 can be specially set according to the characteristics of the chip 200, so that the chip 200 after hot melting will not be drawn, have a smooth surface and a regular shape.

[0093] The length direction of the heating rod 51 can be the same as the arrangement direction of the reagent channels 210, and there can be multiple hot melt heads 52, which are arranged along the length direction of the heating rod 51. The multiple hot melt heads 52 can be arranged in a one-to-one correspondence with the multiple first channels 231, so that each hot melt head 52 can heat-seal the corresponding first channel 231. The multiple hot melt heads 52 can be arranged in a one-to-one correspondence with the multiple second channels 232, so that each hot melt head 52 can heat-seal the corresponding second channel 232.

[0094] In one embodiment, the blocking device 50 includes a plurality of plugs, one plug is disposed in each first hole 221, so that the plug blocks the first channel 231. Alternatively, the blocking device 50 includes two plugs, one plug is disposed in each of the two second holes 222, so that the plug blocks the second channel 232.

[0095] See also Fig.11 and Fig.12 In some embodiments, the hot melt head 52 includes a hot melt block 53 and a clamping block 54 arranged on one side in the height direction of the hot melt block 53. The end faces of the hot melt block 53 and the clamping block 54 are both facing the loading device 30. The end face of the clamping block 54 is a plane, and the end face of the hot melt block 53 is a concave surface that is concave away from the loading device 30.

[0096] Specifically, the size of the end face of the hot melt block 53 can be the same as or different from the size of the end face of the pressing block 54. The degree of concavity of the end face of the hot melt block 53 can be set according to the cross-sectional shape and cross-sectional size of the connecting channel 230. Since the connecting channel 230 is generally a circular channel, the end face of the hot melt block 53 is a concave surface, so that the side wall of the connecting channel 230 is closer to the hot melt block 53, and the end face of the hot melt block 53 is close to each other. The distance between the side wall of the connecting channel 230 is close, so that the heating temperature near the connecting channel 230 is high, the heating range is wide, and the heating is uniform, which is more conducive to the hot melting of the connecting channel 230.

[0097] The length of the pressing block 54 can be smaller than that of the hot melt block 53. When the hot melt head 52 performs hot melting on the chip 200, the hot melt block 53 first softens the side wall of the connecting channel 230 by heat, and moves the molten part to the connecting channel 230 to fill the connecting channel 230. The plane of the pressing block 54 flattens and compacts the hot melt surface, so that the cooling and forming connecting channel 230 has high sealing performance, is not easy to leak, and has a smooth surface and a regular shape, which improves the appearance of the chip 200 and prevents the hot melt block 53 from adhering to the molten material when it is separated from the hot melt surface. The hot melt block 53 can be made of a thermally conductive material, such as copper; the pressing block 54 can be made of a thermally insulating material, such as Teflon.

[0098] See also Figure 1 and Fig.13 In some embodiments, the reagent perfusion device 100 includes a second driving member 65 disposed on the platform 10, and the second driving member 65 is used to drive the heating rod 51 to move relative to the chip 200, and the heating rod 51 drives the hot melt head 52 to move relative to the chip 200 to block the reagent channel 210. The second driving member 65 can provide driving force for the movement of the blocking device 50 through the motor 61, hydraulic pressure, air pump, etc.

[0099] See also Figure 2 and Figure 8 In some embodiments, the second driving member 65 is disposed on the lower surface of the platform 10, the blocking device 50 includes an adapter 55, the adapter 55 is passed through the platform 10, the heating rod 51 is disposed on the adapter 55, the adapter 55 is connected to the second driving member 65, the second driving member 65 is used to drive the adapter 55 to drive the heating rod 51 to move toward the loading device 30, and the heating rod 51 drives the hot melt head 52 to move toward the loading device 30.

[0100] The second driving member 65 is arranged on the lower surface of the platform 10 to save space on the upper surface of the platform 10 and improve the space utilization rate of the platform 10. The second driving member 65 can be a screw motor 61, and the adapter 55 is slidably connected to the output shaft 62 of the motor 61. The axial direction of the output shaft 62 is consistent with the thickness direction of the chip 200. The motor 61 rotates to drive the adapter 55 to move relative to the axial direction of the output shaft 62, thereby driving the heating rod 51 to move closer to or away from the loading device 30, so that the hot melt head 52 is closer to or away from the chip 200. The second driving member 65 can also be connected to the adapter 55 through a transmission device such as a gear, a rack, a conveyor belt, a chain, etc. and output torque.

[0101] See also Figure 8 The lower surface of the platform 10 may be provided with a second detection device 66, which includes a second stopper 67. When the pressing block 54 flattens and compacts the hot melt surface, the adapter 55 contacts the second stopper 67, the second detection device 66 sends a signal, and the second driving member 65 stops working. In some embodiments, the second detection device 66 may be a photoelectric sensor.

[0102] See also Fig.14 In some embodiments, the second driving member 65 , the adapter 55 and the second detecting device 66 may be disposed on the upper surface of the platform 10 .

[0103] In some embodiments, the number of the blocking device 50 and the second driving member 65 is two, and the two second driving members 65 are connected to the two blocking devices 50 in a one-to-one correspondence. The height of the hot melt head 52 of one of the blocking devices 50 is the same as the height of the first channel 231 to block the first channel 231, one of the second driving members 65 is located on the platform 10 and is located on one side of the length direction of the heating rod 51 of one of the blocking devices 50, and the adapter 55 of one of the blocking devices 50 is connected to one end of the length direction of the heating rod 51 of one of the blocking devices 50 and one of the second driving members 65.

[0104] The height of the hot melt head 52 of the other sealing device 50 is the same as the height of the second channel 232 to block the second channel 232. The upper surface of the platform 10 can extend upward to form a boss. The other second driving member 65 is located on the boss and between the heating rods 51 of the two sealing devices 50. The adapter 55 of the other sealing device 50 is connected to the bottom of the heating rod 51 of the other sealing device 50 and the other second driving member 65.

[0105] The two second driving members 65 respectively drive the adapter 55 of the corresponding blocking device 50 to move toward the loading device 30, so as to drive the hot melt head 52 of the corresponding blocking device 50 to move toward the chip 200, so that the reagent filling device 100 has the function of blocking the first channel 231 and the second channel 232 at the same time.

[0106] See also Figure 2 and Figure 3 In some embodiments, the reagent perfusion device 100 includes a heat sink 70 disposed on the platform 10, and the heat sink 70 is used to dissipate heat from the heating rod 51. The heat sink 70 can accelerate the heat dissipation of the heating rod 51 after the heating of the heating rod 51 is completed, improve the heat dissipation efficiency of the heating rod 51, and reduce the impact of the residual heat of the heating rod 51 on the chip 200.

[0107] See also Figure 2 and Figure 3 In some embodiments, the heat dissipation device 70 includes a fan 71 and a housing 72, the heating rod 51 is located in the housing 72, and the fan 71 is located on one side of the heating rod 51. The fan 71 and the housing 72 can be fixed to the upper surface of the platform 10 by fasteners, the housing 72 extends along the fan 71 toward the heating rod 51, and the housing 72 is provided with an air outlet 73, which is located at one end of the housing 72 close to the heating rod 51. The airflow passing through the fan 71 exchanges heat at the heating rod 51 and then is discharged from the air outlet 73.

[0108] See also Figure 3 and Fig.15 In some embodiments, the volume height of the housing 72 tends to decrease from the fan 71 toward the heating rod 51.

[0109] The volume of the housing 72 decreases from the fan 71 to the heating rod 51, so that the airflow passing through the fan 71 can be gathered at the heating rod 51, improving the heat exchange efficiency with the heating rod 51, thereby further accelerating the heat dissipation of the heating rod 51. In addition, the housing 72 with a decreasing volume height can reduce the heat exchange between the heating rod 51 and the surrounding environment when the fan 71 remains closed, reduce heat loss, and help the heating rod 51 to heat the hot melt head 52 more efficiently so that the hot melt head 52 can hot-melt and seal the connecting channel 230, thereby improving the perfusion efficiency of the reagent.

[0110] For example, when the hot melt head 52 is hot melting the first channel 231, the height of the top surface of the housing 72 is from high to low from the direction of the fan 71 to the heating rod 51; when the hot melt head 52 is hot melting the second channel 232, the height of the bottom surface of the housing 72 is from low to high from the direction of the fan 71 to the heating rod 51. The heat dissipation speed and heat dissipation time of the fan 71 can be specially set according to the characteristics of the chip 200.

[0111] See also Figure 2 and Fig.16 In some embodiments, the reagent filling device 100 includes a heating device 21 and a heat preservation device 22 . The heating device 21 is disposed on the side wall of the storage container 20 . The heating device 21 is used to heat the storage container 20 . The heat preservation device 22 wraps the heating device 21 .

[0112] In this way, the heating device 21 heats the storage container 20, and the insulation device 22 insulates the storage container 20, so that the temperature of the storage container 20 is maintained within a preset temperature range, which can prevent the reagent in the storage container 20 from solidifying, thereby improving the efficiency of perfusing the reagent into the chip 200.

[0113] Specifically, the heating device 21 may be a ceramic heating plate, and the heat-insulating device 22 may be a heat-insulating coating applied on the surface of the heating device 21 , or a heat-insulating board or heat-insulating felt wrapped on the surface of the heating device 21 .

[0114] See also Fig.17 In some embodiments, the reagent perfusion device 100 includes a housing 12 and a hot air blower 13 disposed on a platform 10 , a space is formed between the housing 12 and the platform 10 , and the hot air blower 13 is used to heat the space.

[0115] In this way, the hot air blower 13 can provide a heating environment to heat the pipeline, thereby preventing the reagent from solidifying during the transportation process, thereby improving the efficiency of perfusing the chip 200 with the reagent.

[0116] Specifically, the housing 12 and the hot air blower 13 can be fixed to the upper surface of the platform 10 by fasteners, and the housing 12 isolates the components on the upper surface of the platform 10 from the external environment. All the components on the upper surface of the platform 10 are accommodated in the space formed by the housing 12 and the platform 10. The hot air blower 13 is located on the side of the chip 200 away from the blocking device 50 and toward the side of the reagent network 40.

[0117] See also Figure 4 In some embodiments, the reagent perfusion device 100 includes an illumination device 14 disposed on the platform 10 . The illumination device 14 is located on one side of the loading device 30 and is used to illuminate the chip 200 .

[0118] Specifically, the reagent filling device 100 usually uses quantitative reagents for filling. Through the lighting device 14, the human eye can assist in determining the position of the gel and buffer in the reagent channel 210 to prevent excessive or insufficient reagent volume, so as to ensure that the volume of the gel and buffer is within a preset range.

[0119] The lighting device 14 can be fixed to the upper surface of the platform 10 by fasteners. The lighting device 14 is located on one side of the cover plate 202 of the chip 200 . The light beam emitted by the lighting device 14 can pass through the cover plate 202 and irradiate the reagent channel 210 .

[0120] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0121] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A reagent perfusion device, It is characterized in that include: platform; a storage container for storing reagents, wherein the reagents include gel and / or buffer; A loading device, used for clamping the chip, wherein the loading device forms a vertical loading space, the chip is vertically located in the loading space, and the chip is provided with a reagent channel, which extends from bottom to top; a reagent network, the reagent network comprising a pump and a pipeline connected to the pump, the pump being used to pump the reagent in the storage container into the reagent channel through the pipeline; A blocking device, the blocking device is used to block the reagent channel after the reagent is pumped into the reagent channel; Wherein, the storage container, the loading device, the reagent network and the blocking device are all arranged on the platform.

2. The reagent injection device according to claim 1, It is characterized in that The blocking device comprises a heating rod and a hot melt head, one end of the hot melt head is connected to the heating rod, and the other end is used to abut against the chip to block the reagent channel by hot melting.

3. The reagent injection device according to claim 2, It is characterized in that The hot melt head includes a hot melt block and a pressing block arranged on one side of the hot melt block in the height direction, the end surface of the hot melt block and the end surface of the pressing block are both facing the loading device, the end surface of the pressing block is a plane, and the end surface of the hot melt block is a concave surface that is concave away from the loading device.

4. The reagent injection device according to claim 1, It is characterized in that The chip is provided with a liquid inlet and a connecting channel, the liquid inlet is connected to the reagent channel through the connecting channel, the reagent perfusion device includes a pressing block connected to the pipeline, the pressing block includes an interface connected to the liquid inlet, and the pump is used to drive the reagent in the storage container to pass through the interface, the liquid inlet and the connecting channel in sequence and enter the reagent channel.

5. The reagent injection device according to claim 4, It is characterized in that The reagents include gel and cleaning fluid, and there are multiple reagent channels. The connecting channel includes multiple first channels that are connected to the reagent channels in a one-to-one correspondence. The liquid inlet hole includes multiple first holes that are connected to the first channels in a one-to-one correspondence. The interface includes a first interface that is connected to the first hole. The pump is used to drive the gel or the cleaning fluid to pass through the first interface, the first hole and the first channel in sequence and enter the corresponding reagent channel.

6. The reagent injection device according to claim 5, It is characterized in that The interface includes a second interface connected to the pipeline, and the second interface is in one-to-one communication with the first interface.

7. The reagent injection device according to claim 6, It is characterized in that The plurality of second interfaces are arranged in two rows, each row of the second interfaces is arranged along a first direction, and along the first direction, the two rows of the second interfaces are staggeredly distributed, and the first direction is the same as the arrangement direction of the reagent channels.

8. The reagent perfusion device according to claim 7, It is characterized in that The pressing block includes an intersecting first surface and a second surface, and in the direction in which the first surface and the second surface are toward the loading device, the angle formed between the first surface and the second surface is an acute angle; one row of the second interfaces is arranged on the first surface, and another row of the second interfaces is arranged on the second surface.

9. The reagent perfusion device according to claim 4, It is characterized in that The reagents include a buffer solution and a cleaning solution, a plurality of reagent channels are provided, the connecting channel includes a second channel connected to the reagent channel, the liquid inlet hole includes a second hole connected to the second channel, the interface includes a third interface connected to the second hole, and the pump is used to drive the buffer solution or the cleaning solution to pass through the third interface, the second hole and the second channel in sequence and enter the reagent channel.

10. The reagent perfusion device according to claim 9, It is characterized in that There are multiple second channels, and there are two second holes. The first second channel connects one end of the first reagent channel and one of the second holes, the last second channel connects one end of the last reagent channel and another second hole, and the remaining second channels connect two adjacent reagent channels.

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