A method for constructing an experimental model of the effects of 25-hydroxyvitamin D3 on pigs

Abstract

The present invention relates to the technical field of animal-related experimental model construction, and discloses a method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs, including setting up two groups of equal-weight piglet experiments, feeding equal amounts of feed respectively, and adding 25-hydroxyvitamin D3 to one group. The weight of each piglet is obtained through a weighing channel, and the piglet food intake is monitored, and the weight changes of piglets with the same food intake after 90 days are compared to evaluate the effect of 25-hydroxyvitamin D3 on pigs. The weight of each piglet can be obtained before and after each feeding through the weighing channel, and each piglet does not need to be weighed by artificial capture, which greatly improves the convenience of obtaining the weight of the piglets; piglets with the same food intake are obtained for comparison, and the variable of food intake is controlled, so that the specific effect of 25-hydroxyvitamin D3 on pigs can be more accurately evaluated, and the interference of other factors can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of constructing animal-related experimental models, and specifically to a method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs. Background Art

[0002] As an important agricultural animal, the health of pigs directly affects the safety of meat food and economic benefits. By conducting experiments on the effects of certain substances on pigs, we can reveal the effects of substances on pig growth, reproduction, immune response and disease resistance, and provide a scientific basis for feed additives, drug use and disease prevention. In addition, the physiological structure of pigs is similar to that of humans, and the research results can help predict the potential impact of the substance on human health and promote the development of cross-species medical research.

[0003] 25-Hydroxyvitamin D3 is the first metabolite in the vitamin D3 metabolic chain. It participates in the synthesis of calcium-binding proteins in the small intestinal mucosal cells. Vitamin D3 can also promote the flow of calcium ions between cells. However, ordinary vitamin D3 is easily affected by fat, bile, intestinal inflammation or damage, and oxidative factors such as high copper and high iron in the raw materials. The activity of 25-hydroxyvitamin D3 actually produced in the animal body is greatly reduced. Direct intake of 25-hydroxyvitamin D3 simplifies the metabolic process of vitamin D3, utilizes different intestinal absorption patterns and bypasses the liver's transfer process to provide more and more effectively released active metabolites, ensure calcium absorption and pig bone strength, and maximize pig production performance.

[0004] In the experiment of studying the effect of 25-hydroxyvitamin D3 on pigs, the effect of 25-hydroxyvitamin D3 on pigs is generally determined by comparing the weight of pigs and according to the change of the weight of pigs. Therefore, during the experiment, the pigs need to be weighed. It is more common to capture each pig and weigh them separately. The whole weighing process is very time-consuming and it is difficult to weigh frequently. If the weighing times are not sufficient, it is difficult to accurately obtain the weight changes of pigs and obtain more detailed experimental results. In addition, the control of the pigs' food intake is not clear enough, and the food intake of each pig cannot be obtained. When obtaining the experimental results by comparison, the experimental variable of food intake cannot be guaranteed to be consistent, which affects the accuracy of the experimental results. In order to meet the weighing needs, this scheme intends to propose a method that can reduce the time of the weighing process and thus accurately understand the weight changes. Summary of the invention

[0005] The present invention provides a method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs, which helps solve the problems mentioned in the above background technology.

[0006] The present invention provides the following technical solution: a method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs, comprising:

[0007] Set up the first experimental group and the second experimental group, each experimental group contains the same number of piglets of the same weight, and set up a feeding area and a living area for each experimental group;

[0008] A weighing channel was set up between the feeding area and the living area of ​​each experimental group;

[0009] The same amount of feed was placed in the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups;

[0010] The piglets in each experimental group were driven from the living area through the weighing tunnel to the feeding area for feeding;

[0011] S1, obtaining the weight values ​​measured by each weight measuring unit on the weighing channel, and dividing the weight values ​​into a measurement group every 4;

[0012] S2, comparing the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet;

[0013] S3. If the weight values ​​in the measurement group represent the weight of the same piglet, calculate and record the weight of the piglet;

[0014] S4. If the weight values ​​in the measurement group do not represent the weight of the same piglet, obtain the partition position and lower the partition at the partition position;

[0015] S5. After the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded;

[0016] When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel;

[0017] The weight of each piglet after feeding is obtained through the weighing channel, and the food intake of each piglet is calculated;

[0018] After 24 h, the second feeding was carried out, and the weight of each piglet before feeding was measured through the weighing channel;

[0019] Obtain and compare the piglets with the same food intake in the first experimental group and the second experimental group, and obtain the weight change of the piglets with the same food intake after 24 hours;

[0020] The experiment was carried out for 90 days. After 90 days, the piglets in the first experimental group and the second experimental group with the same food intake were compared to obtain the effect of 25-hydroxyvitamin D3 on pigs.

[0021] Optionally, a weighing channel is set between the feeding area and the living area of ​​each experimental group, specifically:

[0022] A weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the first experimental group;

[0023] A weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the second experimental group;

[0024] The same amount of feed was respectively put into the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups, specifically:

[0025] Feed supplemented with 25-hydroxyvitamin D3 was placed in the feeding area of ​​the first experimental group;

[0026] The same amount of feed without 25-hydroxyvitamin D3 was placed in the feeding area of ​​the second experimental group.

[0027] Optionally, the step of obtaining the weight values ​​measured by each weight measurement unit on the weighing channel and dividing the weight values ​​into a measurement group every 4 units specifically includes:

[0028] Multiple independent weight measuring units are arranged on the weighing channel;

[0029] Obtain the weight measurement unit of the weight value measured on the weighing channel and record it as the effective measurement unit;

[0030] The direction in which the piglets move is regarded as the positive direction, and the valid measuring units existing on the weighing channel are divided into a group of 4 in the opposite direction of the positive direction, which is recorded as a measuring group;

[0031] Get the weight values ​​measured by the effective measuring units in each measurement group respectively.

[0032] Optionally, comparing the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet specifically includes:

[0033] Get the average weight of all piglets, recorded as D;

[0034] Obtain the total weight of feed added to the feeding area each time, recorded as G;

[0035] Get the number of all piglets, recorded as B;

[0036] The average weight of feed fed to each piglet is calculated as S = G ÷ B;

[0037] The average weight of each piglet after eating was recorded as N;

[0038] The result of D+S is used as the value of N;

[0039] Calculate the sum of the 4 weight values ​​in each measurement group and record the result as P;

[0040] Set the deviation threshold A;

[0041] When the piglets enter the eating area from the living area through the weighing channel to eat, for the measurement group on the weighing channel;

[0042] If the absolute value of the difference between P and D is greater than A, it is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and D is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet.

[0043] When the piglets enter the living area from the feeding area through the weighing tunnel, for the measurement group on the weighing tunnel;

[0044] If the absolute value of the difference between P and N is greater than A, it is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and N is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet.

[0045] Optionally, if the weight values ​​in the measurement group represent the weight of the same piglet, the weight of the piglet is calculated and recorded, specifically:

[0046] If the weight values ​​in a measurement group represent the weight of the same piglet, the measurement group is recorded as a valid measurement group;

[0047] Calculate the sum of the 4 weight values ​​in the valid measurement group, and the result is the weight of the piglets measured in the valid measurement group;

[0048] If the weight values ​​in the measurement group do not represent the weight of the same piglet, the partition position is obtained and the partition is lowered at the partition position, specifically:

[0049] Obtain the valid measurement units corresponding to the 4 weight values ​​in the measurement group, and obtain the positions of the 4 valid measurement units on the weighing channel;

[0050] Taking the direction of the piglet's movement as the positive direction, the four effective measuring units are sorted in front and back order according to their positions on the weighing channel;

[0051] Get the position of the first valid measurement unit, which is recorded as position one;

[0052] Taking the direction of the piglet's movement as the positive direction, obtain the position of a valid measurement unit adjacent to position 1 and before position 1, and record it as position 2;

[0053] Get the middle position between the first and second positions as the separator;

[0054] The control partition is lowered to the separation position to block the weighing channel.

[0055] Optionally, after the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded, specifically:

[0056] Keep the partition in the lowered position;

[0057] Taking the direction of the piglet's movement as the positive direction, starting from the location of the partition, obtain the first four weight measurement units that measure the weight values ​​in front of the partition on the weighing channel, and record them as the second measurement group;

[0058] The sum of the weight values ​​measured by the weight measuring units in the second measurement group is obtained, and the result is the weight of the piglet.

[0059] Optionally, obtaining the weight of each piglet after feeding through a weighing channel and calculating the food intake of each piglet specifically includes:

[0060] When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel;

[0061] Using steps S1-S5, the weight of each piglet after feeding was obtained through the weighing channel;

[0062] The weight of each piglet before feeding was recorded as the initial weight;

[0063] The weight of each piglet after feeding was recorded as the final weight;

[0064] Subtract the initial weight from the final weight of each piglet to obtain the food intake of the piglet.

[0065] Optionally, after 24 hours, a second feeding is performed, and the weight of each piglet before feeding is measured through a weighing channel, specifically including:

[0066] After 24 h, the piglets in each experimental group were driven from the living area through the weighing channel to the feeding area for the second feeding;

[0067] Using steps S1-S5, the weight of each piglet before the second feeding was obtained through the weighing channel and recorded as the No. 2 initial weight.

[0068] Optionally, the method of obtaining and comparing the piglets with the same food intake in the first experimental group and the second experimental group, and obtaining the weight change of the piglets with the same food intake after 24 hours, is specifically as follows:

[0069] Obtain the food intake of each piglet in the first experimental group, and obtain the food intake of each piglet in the second experimental group;

[0070] Obtain the difference between the No. 2 initial weight and the initial weight of each piglet, and record it as the weight change of the piglet;

[0071] The piglets with the same food intake in the first and second experimental groups were divided into a comparison group;

[0072] In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group were compared to obtain the effect of 25-hydroxyvitamin D3 on the daily weight of pigs;

[0073] The experiment was carried out for 90 days, and the piglets with the same food intake in the first experimental group and the second experimental group were compared after 90 days to obtain the effect of 25-hydroxyvitamin D3 on pigs, specifically:

[0074] During the 90 days of the experiment, the food intake of each piglet was recorded every 24 h;

[0075] By recording the food intake of each piglet every 24 hours, the total food intake of each piglet after 90 days was calculated;

[0076] The piglets with the same total food intake in the first and second experimental groups were divided into a comparison group;

[0077] In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group after 90 days were compared to obtain the effect of 25-hydroxyvitamin D3 on pigs.

[0078] The present invention has the following beneficial effects:

[0079] 1. In the experiment, the living area and feeding area of ​​the piglets were separated, and a weighing channel was set up between the two areas. The weight of each piglet could be obtained before and after each feeding through the weighing channel. There was no need to weigh each piglet manually, which greatly improved the convenience of obtaining the weight of the piglets. The piglets could be weighed before and after each feeding, which increased the number of weighings and enabled detailed monitoring of the daily weight changes of the piglets, so as to gain a more detailed understanding of the effects of 25-hydroxyvitamin D3 on pigs.

[0080] 2. By feeding the same amount of feed each time, the amount of food intake by the piglets in the two experimental groups is controlled to be basically the same each time, eliminating the influence of different food intake on the experimental results, and more accurately evaluating the effect of 25-hydroxyvitamin D3 on pigs.

[0081] 3. Obtain the weight changes of piglets with the same food intake every 24 hours, and accurately measure the specific effects of 25-hydroxyvitamin D3 on pigs to the day, so as to understand the weight change trend in more detail and monitor the weight changes in real time. In addition, daily records can more accurately evaluate the specific effects of substances on body weight, especially when weight changes are not obvious in the short term. Moreover, by reducing the time interval for data collection, the measurement errors caused by long time intervals can be reduced.

[0082] 4. Obtaining piglets with the same food intake for comparison and controlling the variable of food intake can more accurately evaluate the specific effects of 25-hydroxyvitamin D3 on pigs, reduce interference from other factors, avoid weight changes caused by different food intake, and reduce deviations in experimental results.

[0083] 5. An independent weight measurement unit is set on the weighing channel to obtain the weight of each piglet. By comparing the weight values ​​measured by the measuring unit, it can be determined whether the piglets are pushed and overlapped when passing through the weighing channel. The position of the overlap can be obtained based on the position of the measuring unit, so that the overlapping piglets can be separated to obtain the accurate weight of the piglets. BRIEF DESCRIPTION OF THE DRAWINGS

[0084] Figure 1 It is a schematic diagram of the position of the weighing channel of the present invention.

[0085] Figure 2 This is a schematic diagram of the measurement group division of the present invention.

[0086] Figure 3 It is a schematic diagram of the location of the separation position of the present invention. DETAILED DESCRIPTION

[0087] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0088] Embodiment, a method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs, comprising:

[0089] Set up the first experimental group and the second experimental group, each experimental group contains the same number of piglets of the same weight, and set up a feeding area and a living area for each experimental group;

[0090] A weighing channel was set up between the feeding area and the living area of ​​each experimental group. In the experiment, the living area and the feeding area of ​​the piglets were separated, and a weighing channel was set up between the two areas. The weight of each piglet could be obtained before and after each feeding through the weighing channel, and there was no need to weigh each piglet manually, which greatly improved the convenience of obtaining the weight of the piglets. The piglets could be weighed before and after each feeding, which increased the number of weighings, enabled detailed monitoring of the daily weight changes of the piglets, and provided a more detailed understanding of the effects of 25-hydroxyvitamin D3 on pigs.

[0091] An equal amount of feed was placed in the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups. By placing an equal amount of feed each time, the amount of food eaten by the piglets in the two experimental groups was kept basically the same, eliminating the impact of different feed amounts on the experimental results and more accurately evaluating the effect of 25-hydroxyvitamin D3 on pigs.

[0092] The piglets in each experimental group were driven from the living area through the weighing tunnel to the feeding area for feeding;

[0093] S1. Obtain the weight values ​​measured by each weight measuring unit on the weighing channel, and divide every 4 weight values ​​into a measurement group; pigs are animals that walk on four legs. For a piglet, when it passes through the weighing channel, it will generate pressure on 4 positions. Therefore, the weight values ​​are divided into a measurement group every 4. Under normal circumstances, each measurement group is the pressure generated by a piglet.

[0094] S2. Compare the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet. The weighing channel only allows one piglet to pass through. However, considering that piglets may push and shove when passing through the weighing channel, causing two adjacent piglets to overlap half of their bodies, the four weight values ​​in a measurement group do not represent the weight of one piglet.

[0095] S3. If the weight values ​​in the measurement group represent the weight of the same piglet, calculate and record the weight of the piglet;

[0096] S4. If the weight values ​​in the measurement group do not represent the weight of the same piglet, the separation position is obtained and the partition is lowered at the separation position; by obtaining the overlapping position of the two overlapping piglets, the two piglets are separated by the partition at this position, and the weight of each piglet after separation is obtained again.

[0097] S5. After the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded;

[0098] When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel;

[0099] The weight of each piglet after feeding is obtained through the weighing channel, and the food intake of each piglet is calculated;

[0100] After 24 h, the second feeding was carried out, and the weight of each piglet before feeding was measured through the weighing channel;

[0101] The piglets with the same food intake in the first experimental group and the second experimental group were compared, and the weight change of the piglets with the same food intake after 24 hours was obtained; the weight change of piglets with the same food intake was obtained every 24 hours, and the specific effect of 25-hydroxyvitamin D3 on pigs was accurately calculated to the day, so as to understand the trend of body weight changes in more detail and monitor the changes in body weight in real time; in addition, daily records can more accurately evaluate the specific effects of substances on body weight, especially when the weight changes are not obvious in the short term; and by reducing the time interval for data collection, the measurement errors caused by long time intervals can be reduced.

[0102] The experiment was carried out for 90 days, and after 90 days, the piglets in the first and second experimental groups with the same food intake were compared to obtain the effect of 25-hydroxyvitamin D3 on pigs. By comparing piglets with the same food intake and controlling the variable of food intake, the specific effect of 25-hydroxyvitamin D3 on pigs can be more accurately evaluated, the interference of other factors can be reduced, the weight change caused by different food intake can be avoided, and the deviation of experimental results can be reduced.

[0103] The weighing channel is set between the feeding area and the living area of ​​each experimental group, specifically:

[0104] Reference Figure 1 , a weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the first experimental group;

[0105] A weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the second experimental group;

[0106] The width of the weighing channel is related to the size of the piglets. The width of the weighing channel only allows one piglet to pass through. When the piglets pass through the weighing channel, they line up in a row and pass in turn.

[0107] The same amount of feed was respectively put into the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups, specifically:

[0108] Feed supplemented with 25-hydroxyvitamin D3 was placed in the feeding area of ​​the first experimental group;

[0109] The same amount of feed without 25-hydroxyvitamin D3 was placed in the feeding area of ​​the second experimental group.

[0110] The step of obtaining the weight values ​​measured by each weight measuring unit on the weighing channel and dividing the weight values ​​into a measurement group every 4 units specifically includes:

[0111] Multiple independent weight measuring units are arranged on the weighing channel;

[0112] Obtain the weight measurement unit of the weight value measured on the weighing channel and record it as the effective measurement unit;

[0113] Reference Figure 2 , taking the direction of the piglet's movement as the positive direction, and dividing the valid measuring units on the weighing channel into a group of 4 in the opposite direction of the positive direction, which is recorded as a measuring group;

[0114] Get the weight values ​​measured by the effective measuring units in each measurement group respectively.

[0115] The comparing of the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet specifically includes:

[0116] Get the average weight of all piglets, recorded as D;

[0117] Obtain the total weight of feed added to the feeding area each time, recorded as G;

[0118] Get the number of all piglets, recorded as B;

[0119] The average weight of feed fed to each piglet is calculated as S = G ÷ B;

[0120] The average weight of each piglet after eating was recorded as N;

[0121] The result of D+S is used as the value of N;

[0122] Calculate the sum of the 4 weight values ​​in each measurement group and record the result as P;

[0123] Set the deviation threshold A; A represents the maximum difference between the weight of the piglets represented by the measurement group and the average weight of the piglets when judging whether the weight values ​​in the measurement group represent the weight of the same piglet. The smaller the A value, the smaller the difference between the weight of the piglets represented by the measurement group and the average weight of the piglets. When A is equal to 0, when judging whether the weight values ​​in the measurement group represent the weight of the same piglet, the weight of the piglets measured by the measurement group must be equal to the average weight, and only then can it be determined that the measurement group represents the weight of the same piglet.

[0124] When the piglets enter the eating area from the living area through the weighing channel to eat, for the measurement group on the weighing channel;

[0125] If the absolute value of the difference between P and D is greater than A, it means that the weight of the piglets obtained by the measurement group is much greater than the average weight of the piglets in the experimental group, and the weight data obtained is unreasonable. It is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and D is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet.

[0126] When the piglets enter the living area from the feeding area through the weighing tunnel, for the measurement group on the weighing tunnel;

[0127] If the absolute value of the difference between P and N is greater than A, it means that the weight of the piglets obtained by the measurement group is much greater than the average weight of the piglets in the experimental group after eating. The obtained weight data is unreasonable. It is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and N is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet.

[0128] If the weight values ​​in the measurement group represent the weight of the same piglet, the weight of the piglet is calculated and recorded, specifically:

[0129] If the weight values ​​in a measurement group represent the weight of the same piglet, the measurement group is recorded as a valid measurement group;

[0130] Calculate the sum of the 4 weight values ​​in the valid measurement group, and the result is the weight of the piglets measured in the valid measurement group;

[0131] If the weight values ​​in the measurement group do not represent the weight of the same piglet, the partition position is obtained and the partition is lowered at the partition position, specifically:

[0132] Obtain the valid measurement units corresponding to the 4 weight values ​​in the measurement group, and obtain the positions of the 4 valid measurement units on the weighing channel;

[0133] Reference Figure 3 , taking the direction of the piglet's movement as the positive direction, the four effective measuring units are sorted in front and back order according to their positions on the weighing channel;

[0134] Get the position of the first valid measurement unit, which is recorded as position one;

[0135] Taking the direction of the piglet's movement as the positive direction, obtain the position of a valid measurement unit adjacent to position 1 and before position 1, and record it as position 2;

[0136] Get the middle position between the first and second positions as the separator;

[0137] The control partition is lowered to the separation position to block the weighing channel.

[0138] After the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded, specifically:

[0139] Keep the partition in the lowered state; at this time, separate the two overlapping piglets. The weight values ​​measured by the 4 effective measurement units in front of the partition represent the weight of one piglet.

[0140] Taking the direction of the piglet's movement as the positive direction, starting from the location of the partition, obtain the first four weight measurement units that measure the weight values ​​in front of the partition on the weighing channel, and record them as the second measurement group;

[0141] The sum of the weight values ​​measured by the weight measuring units in the second measurement group is obtained, and the result is the weight of the piglet.

[0142] The weight of each piglet after feeding is obtained through the weighing channel, and the food intake of each piglet is calculated, specifically including:

[0143] When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel;

[0144] Using steps S1-S5, the weight of each piglet after feeding was obtained through the weighing channel;

[0145] The weight of each piglet before feeding was recorded as the initial weight;

[0146] The weight of each piglet after feeding was recorded as the final weight;

[0147] Subtract the initial weight from the final weight of each piglet to obtain the food intake of the piglet.

[0148] After 24 hours, the second feeding was carried out, and the weight of each piglet before feeding was measured through the weighing channel, specifically including:

[0149] After 24 h, the piglets in each experimental group were driven from the living area through the weighing channel to the feeding area for the second feeding;

[0150] Using steps S1-S5, the weight of each piglet before the second feeding is obtained through the weighing channel, which is recorded as the second initial weight. The weight of each piglet obtained at this time is the weight after digesting the feed fed the previous day.

[0151] The piglets with the same food intake in the first experimental group and the second experimental group were compared to obtain the weight change of the piglets with the same food intake after 24 hours, specifically:

[0152] Obtain the food intake of each piglet in the first experimental group, and obtain the food intake of each piglet in the second experimental group;

[0153] Obtain the difference between the No. 2 initial weight and the initial weight of each piglet, and record it as the weight change of the piglet;

[0154] The piglets with the same food intake in the first and second experimental groups were divided into a comparison group;

[0155] In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group were compared to obtain the effect of 25-hydroxyvitamin D3 on the daily weight of pigs;

[0156] The experiment was carried out for 90 days, and the piglets with the same food intake in the first experimental group and the second experimental group were compared after 90 days to obtain the effect of 25-hydroxyvitamin D3 on pigs, specifically:

[0157] During the 90 days of the experiment, the food intake of each piglet was recorded every 24 h;

[0158] By recording the food intake of each piglet every 24 hours, the daily food intake of each piglet was added up to calculate the total food intake of each piglet after 90 days;

[0159] The piglets with the same total food intake in the first and second experimental groups were divided into a comparison group;

[0160] In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group after 90 days were compared to obtain the effect of 25-hydroxyvitamin D3 on pigs.

[0161] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0162] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs, characterized in that: include: Set up the first experimental group and the second experimental group, each experimental group contains the same number of piglets of the same weight, and set up a feeding area and a living area for each experimental group; A weighing channel was set up between the feeding area and the living area of ​​each experimental group; The same amount of feed was placed in the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups; The piglets in each experimental group were driven from the living area through the weighing tunnel to the feeding area for feeding; S1, obtaining the weight values ​​measured by each weight measuring unit on the weighing channel, and dividing the weight values ​​into a measurement group every 4; S2, comparing the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet; S3. If the weight values ​​in the measurement group represent the weight of the same piglet, calculate and record the weight of the piglet; S4. If the weight values ​​in the measurement group do not represent the weight of the same piglet, obtain the partition position and lower the partition at the partition position; S5. After the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded; When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel; The weight of each piglet after feeding is obtained through the weighing channel, and the food intake of each piglet is calculated; After 24 h, the second feeding was carried out, and the weight of each piglet before feeding was measured through the weighing channel; Obtain and compare the piglets with the same food intake in the first experimental group and the second experimental group, and obtain the weight change of the piglets with the same food intake after 24 hours; The experiment was carried out for 90 days, and after 90 days, the piglets in the first experimental group and the second experimental group with the same amount of food intake were compared to obtain the results of the effect of 25-hydroxyvitamin D3 on pigs; The step of obtaining the weight values ​​measured by each weight measuring unit on the weighing channel and dividing the weight values ​​into a measurement group every 4 weight values ​​specifically includes: Multiple independent weight measuring units are arranged on the weighing channel; Obtain the weight measurement unit of the weight value measured on the weighing channel and record it as the effective measurement unit; The direction in which the piglets move is regarded as the positive direction, and the valid measuring units existing on the weighing channel are divided into a group of 4 in the opposite direction of the positive direction, which is recorded as a measuring group; Respectively obtain the weight value measured by the effective measurement unit in each measurement group; The comparing of the four weight values ​​in each measurement group to determine whether the weight values ​​in the measurement group represent the weight of the same piglet specifically includes: Get the average weight of all piglets, recorded as D; Obtain the total weight of feed added to the feeding area each time, recorded as G; Get the number of all piglets, recorded as B; The average weight of feed fed to each piglet is calculated as S = G ÷ B; The average weight of each piglet after eating was recorded as N; The result of D+S is used as the value of N; Calculate the sum of the 4 weight values ​​in each measurement group and record the result as P; Set the deviation threshold A; When the piglets enter the eating area from the living area through the weighing channel to eat, for the measurement group on the weighing channel; If the absolute value of the difference between P and D is greater than A, it is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and D is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet. When the piglets enter the living area from the feeding area through the weighing tunnel, for the measurement group on the weighing tunnel; If the absolute value of the difference between P and N is greater than A, it is determined that the weight values ​​in the measurement group do not represent the weight of the same piglet. If the absolute value of the difference between P and N is less than or equal to A, it is determined that the weight values ​​in the measurement group represent the weight of the same piglet.

2. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 1, characterized in that: The weighing channel is set between the feeding area and the living area of ​​each experimental group, specifically: A weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the first experimental group; A weighing passage connecting the feeding area and the living area was set between the feeding area and the living area of ​​the second experimental group; The same amount of feed was respectively put into the feeding areas of the two experimental groups, and 25-hydroxyvitamin D3 was added to the feed in the feeding area of ​​one of the experimental groups, specifically: Feed supplemented with 25-hydroxyvitamin D3 was placed in the feeding area of ​​the first experimental group; The same amount of feed without 25-hydroxyvitamin D3 was placed in the feeding area of ​​the second experimental group.

3. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 1, characterized in that: If the weight values ​​in the measurement group represent the weight of the same piglet, the weight of the piglet is calculated and recorded, specifically: If the weight values ​​in a measurement group represent the weight of the same piglet, the measurement group is recorded as a valid measurement group; Calculate the sum of the 4 weight values ​​in the valid measurement group, and the result is the weight of the piglets measured in the valid measurement group; If the weight values ​​in the measurement group do not represent the weight of the same piglet, the partition position is obtained and the partition is lowered at the partition position, specifically: Obtain the valid measurement units corresponding to the 4 weight values ​​in the measurement group, and obtain the positions of the 4 valid measurement units on the weighing channel; Taking the direction of the piglet's movement as the positive direction, the four effective measuring units are sorted in front and back order according to their positions on the weighing channel; Get the position of the first valid measurement unit, which is recorded as position one; Taking the direction of the piglet's movement as the positive direction, obtain the position of a valid measurement unit adjacent to position 1 and before position 1, and record it as position 2; Get the middle position between the first and second positions as the separator; The control partition is lowered to the separation position to block the weighing channel.

4. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 1, characterized in that: After the partition is lowered, the weight value measured by the weight measuring unit is obtained again, and the weight of the piglet is calculated and recorded, specifically: Keep the partition in the lowered position; Taking the direction of the piglet's movement as the positive direction, starting from the location of the partition, obtain the first four weight measurement units that measure the weight values ​​in front of the partition on the weighing channel, and record them as the second measurement group; The sum of the weight values ​​measured by the weight measuring units in the second measurement group is obtained, and the result is the weight of the piglet.

5. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 1, characterized in that: The weight of each piglet after feeding is obtained through the weighing channel, and the food intake of each piglet is calculated, specifically including: When feeding was finished, the piglets in each experimental group were driven from the feeding area to the living area through the weighing tunnel; Using steps S1-S5, the weight of each piglet after feeding was obtained through the weighing channel; The weight of each piglet before feeding was recorded as the initial weight; The weight of each piglet after feeding was recorded as the final weight; Subtract the initial weight from the final weight of each piglet to obtain the food intake of the piglet.

6. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 5, characterized in that: After 24 hours, the second feeding was carried out, and the weight of each piglet before feeding was measured through the weighing channel, specifically including: After 24 h, the piglets in each experimental group were driven from the living area through the weighing channel to the feeding area for the second feeding; Using steps S1-S5, the weight of each piglet before the second feeding was obtained through the weighing channel and recorded as the second initial weight.

7. The method for constructing an experimental model of the effect of 25-hydroxyvitamin D3 on pigs according to claim 6, characterized in that: The piglets with the same food intake in the first experimental group and the second experimental group were compared to obtain the weight change of the piglets with the same food intake after 24 hours, specifically: Obtain the food intake of each piglet in the first experimental group, and obtain the food intake of each piglet in the second experimental group; Obtain the difference between the No. 2 initial weight and the initial weight of each piglet, and record it as the weight change of the piglet; The piglets with the same food intake in the first and second experimental groups were divided into a comparison group; In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group were compared to obtain the effect of 25-hydroxyvitamin D3 on the daily weight of pigs; The experiment was carried out for 90 days, and the piglets with the same food intake in the first experimental group and the second experimental group were compared after 90 days to obtain the effect of 25-hydroxyvitamin D3 on pigs, specifically: During the 90 days of the experiment, the food intake of each piglet was recorded every 24 h; By recording the food intake of each piglet every 24 hours, the total food intake of each piglet after 90 days was calculated; The piglets with the same total food intake in the first and second experimental groups were divided into a comparison group; In the comparison group, the weight changes of the piglets in the first experimental group and the piglets in the second experimental group after 90 days were compared to obtain the effect of 25-hydroxyvitamin D3 on pigs.

Images