An oil-control and moisturizing composition, its preparation method and application

Abstract

The present invention relates to an oil-control and moisturizing composition, its preparation method and application, belonging to technical field A. An oil-control and moisturizing composition comprises the following components: white flower leaf extract, mangosteen peel extract, eryngium maritimum extract, carboxymethyl chitosan, a moisturizing agent, a skin conditioner, a chelating agent, a preservative, a pH regulator and deionized water. By combining mangosteen peel extract, eryngium maritimum extract, white flower leaf extract and carboxymethyl chitosan, the present invention can effectively reduce the level of lipid peroxidation, enhance the oxidative stress ability, reduce the blockage of pores by lipid peroxidation products, facilitate the entry of moisturizing factors into the skin, and achieve a significant oil-control and moisturizing effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to an oil-control and moisture-retaining composition, a preparation method thereof, and an application thereof. Background Art

[0002] The sebaceous gland, as a key component of the skin, shoulders the important task of secreting sebum. However, when sebum secretion exceeds an appropriate amount, it not only affects beauty but may also induce a series of skin problems. The causes of abnormal sebaceous gland function are quite complex, and the pathological changes it causes are also diverse. Sebum secretion is affected by multiple factors, including endocrine status, skin humidity, age, gender, and diet and nutrition. When the sebaceous gland is overly active, in addition to causing enlarged pores and oily skin, it may also trigger skin diseases such as acne and seborrheic dermatitis. Therefore, regulating sebaceous gland secretion and maintaining oil balance are crucial for maintaining a healthy skin state.

[0003] In the current market, many oil-control products mainly relieve oily skin problems through single means such as cleaning oil, moisturizing, and shrinking pores. Although these methods can improve the skin condition to a certain extent, they do not fundamentally solve the problem of sebaceous gland imbalance. To achieve a radical cure, it is necessary to scientifically and reasonably condition the imbalanced sebaceous gland. Summary of the Invention

[0004] The purpose of the present invention is to provide an oil-control and moisture-retaining composition, a preparation method thereof, and an application thereof. Through the combination of mangosteen pericarp extract, eryngium maritimum extract, white flower leaf extract, and carboxymethyl chitosan, the present invention can effectively reduce the level of lipid peroxidation, enhance the oxidative stress ability, reduce the blockage of pores by lipid peroxidation products, facilitate the entry of moisturizing factors into the skin, and achieve a significant oil-control and moisture-retaining effect.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] An oil-control and moisture-retaining composition, comprising the following components in parts by weight:

[0007]

[0008]

[0009] As a preferred technical solution of the present invention, the oil-control and moisture-retaining composition further comprises the following components in parts by weight: 15 - 24 parts of a moisturizer, 0.1 - 0.2 parts of a skin conditioner, 0.1 - 0.3 parts of a chelating agent, 0.2 - 0.3 parts of a preservative, 0.1 - 0.3 parts of a pH regulator, and 50 - 60 parts of deionized water.

[0010] As a preferred technical solution of the present invention, the moisturizer is one or more of betaine, glycerol, erythritol, and xylitol.

[0011] As a preferred technical solution of the present invention, the humectant is composed of betaine, glycerol, erythritol and xylitol mixed in a mass ratio of 2:5:0.6:0.8.

[0012] As a preferred technical solution of the present invention, the skin conditioner is one or both of ceramide and cholesterol.

[0013] As a preferred technical solution of the present invention, the chelating agent is disodium EDTA; the preservative is one or more of sorbic acid, sodium sorbate and potassium sorbate.

[0014] As a preferred technical solution of the present invention, the pH regulator is one or more of disodium hydrogen phosphate, citric acid and sodium citrate.

[0015] As a preferred technical solution of the present invention, the extract of Eryngium maritimum is rich in flavonoids and phenolic acids, has the ability to scavenge free radicals and chelate metal ions, and effectively reduces the level of lipid peroxidation by enhancing the activities of SOD enzyme, CAT enzyme and GPx enzyme, thereby strengthening the oxidative stress defense mechanism of organisms.

[0016] As a preferred technical solution of the present invention, the extract of Brassica oleracea var. botrytis contains phenolic acids and rutin, which can not only reduce sebum secretion but also inhibit the proliferation of bacteria by promoting the expression of antimicrobial peptides in keratinocytes, and reduce the production of ROS through the antioxidant effect of rutin, thereby improving sebum quality and reducing lipid peroxidation.

[0017] As a preferred technical solution of the present invention, the xanthones rich in the extract of Garcinia mangostana pericarp belong to flavonoid compounds, have good antioxidant, free radical scavenging and antibacterial abilities, and have certain cytotoxicity, and can promote apoptosis. The extract of Garcinia mangostana pericarp has good antioxidant activity, antibacterial, anti-inflammatory and anti-allergic effects.

[0018] As a preferred technical solution of the present invention, positively charged carboxymethyl chitosan can interact with the charged bacterial cell membrane. Through this interaction, carboxymethyl chitosan can effectively adsorb and precipitate bacteria, and further penetrate the cell wall into the bacteria. During this process, it can disrupt the normal metabolism and synthesis process of bacteria.

[0019] Furthermore, due to the high content of flavonoids and phenols in the mangosteen pericarp extract and Eryngium maritimum extract, the present invention has a strong ability to promote free radical scavenging activity. The mangosteen pericarp extract, in combination with carboxymethyl chitosan, interacts with the charged cell membranes of bacteria, significantly enhancing the antibacterial effect against Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus pyogenes, and reducing inflammatory factors caused by bacterial proliferation. The Eryngium maritimum extract, on the other hand, increases the activities of antioxidant enzymes such as SOD enzyme, CAT enzyme, and GPx enzyme. Combining with the phenolic acids and rutin contained in the Brassica oleracea var. acephala leaf extract, it can not only reduce sebum secretion, but also inhibit bacterial proliferation by promoting the expression of antimicrobial peptides in keratinocytes. Coupled with the antioxidant effect of rutin to reduce the production of ROS, thereby improving the quality of sebum. That is, the combination of mangosteen pericarp extract, Eryngium maritimum extract, Brassica oleracea var. acephala leaf extract, and carboxymethyl chitosan can effectively reduce the level of lipid peroxidation, enhance the oxidative stress ability, reduce the blockage of pores by lipid peroxidation products, facilitate the entry of moisturizing factors into the skin, and achieve the effect of oil control and moisturization.

[0020] In addition, the mangosteen pericarp extract has a good antihistamine effect and can reduce allergic reactions, thereby avoiding excessive secretion of sebaceous glands caused by inflammatory stimulation. Moreover, the aldehyde group in the Eryngium maritimum extract reacts with the amino group of carboxymethyl chitosan to form a jelly-like moist gel, which is suitable for topical application on the skin surface and has good skin compatibility. Carboxymethyl chitosan has good water solubility. By adding a moisturizer, it can form a moisturizing film on the skin surface, which can effectively lock the moisture on the skin surface. Combining with the action of moisturizing factors, it can effectively prevent the evaporation of skin moisture, thus achieving the moisturizing effect.

[0021] The present invention discloses a preparation method of an oil-control and moisturizing composition, and the preparation method includes the following steps:

[0022] S1. After heating deionized water to 75 - 85°C, add a moisturizer and a chelating agent and stir and mix at 600 - 800 r / min;

[0023] S2. Cool down to 60 - 65°C, add carboxymethyl chitosan, a preservative, and a skin conditioner, and stir evenly;

[0024] S3. Cool down to 45 - 50°C, add Brassica oleracea var. acephala leaf extract, mangosteen pericarp extract, Eryngium maritimum extract, carboxymethyl chitosan, and a pH regulator, keep stirring at 35 - 45°C, and filter to obtain the oil-control and moisturizing composition.

[0025] The present invention discloses the application of an oil-control and moisturizing composition as described above in cosmetics.

[0026] The preparation method of the mangosteen peel extract disclosed by the present invention comprises the following steps: adding mangosteen peel powder into an ethanol solution, mixing, subjecting to microwave extraction, taking the filtrate for reduced pressure concentration, and performing freeze drying to obtain the mangosteen peel extract.

[0027] In the preparation method of the mangosteen peel extract, the dosage ratio of the mangosteen peel powder to the ethanol solution is 120 - 130 mL:5 g; the mass fraction of the ethanol solution is 60 - 70%; the microwave extraction is to microwave for 2 - 3 min at 80 - 120 W and then extract at 65 - 70°C for 2 - 4 h; the temperature of the reduced pressure concentration is 50°C.

[0028] As a preferred technical solution of the present invention, the mangosteen peel powder is obtained by cutting the cleaned mangosteen peel into pieces, drying at 60°C, and then grinding and pulverizing.

[0029] The present invention discloses a preparation method of the white flower leaf extract, comprising the following steps:

[0030] 1) Adding an ethanol solution to the white flower leaf particles, extracting multiple times, centrifuging, and combining the supernatant to obtain the white flower leaf supernatant;

[0031] 2) Performing reduced pressure concentration on the white flower leaf supernatant to obtain the concentrated white flower leaf supernatant;

[0032] 3) Adding ethanol to the concentrated white flower leaf supernatant, refrigerating at low temperature, centrifuging, and discarding the supernatant to obtain the white flower leaf extract.

[0033] Among them, the white flower leaf particles are obtained by drying, pulverizing, and sieving the white flower leaves through a 40 - mesh sieve;

[0034] As a preferred technical solution of the present invention, in step 1), the dosage ratio of the white flower leaves to the ethanol solution is 1 g:30 - 35 mL; the multiple extractions are performed at 60 - 70°C for 2 - 3 times; the centrifuging conditions are centrifuging at a rotation speed of 5000 - 6000 r / min for 8 - 12 min;

[0035] As a preferred technical solution of the present invention, in step 2), the reduced pressure concentration conditions are concentration at 55 - 60°C.

[0036] As a preferred technical solution of the present invention, in step 3), the volume ratio of the concentrated white flower leaf supernatant to the ethanol is 1:4.0 - 4.5; the ethanol is anhydrous ethanol, the low - temperature refrigeration is refrigeration at 4°C; the centrifuging conditions are centrifuging at 6000 - 7000 r / min for 8 - 12 min.

[0037] The present invention discloses a method for preparing the extract of Eryngium maritimum, comprising the following steps: mixing the powder of Eryngium maritimum and an ethanol solution, heating under reflux, performing vacuum filtration, taking the filtrate for rotary evaporation, drying, and cooling to room temperature to obtain the extract of Eryngium maritimum.

[0038] In the method for preparing the extract of Eryngium maritimum, the dosage ratio of the powder of Eryngium maritimum and the ethanol solution is 1 g: 30 - 40 mL; the mass fraction of the ethanol solution is 30 - 40%; the heating under reflux is at a temperature of 60 - 70 °C and reflux for 4 - 6 h.

[0039] The beneficial effects of the present invention:

[0040] Through the combination of the extract of mangosteen pericarp, the extract of Eryngium maritimum, the extract of white flower leaf, and carboxymethyl chitosan, the present invention can effectively reduce the level of lipid peroxidation, enhance the oxidative stress ability, reduce the blockage of pores by lipid peroxidation products, facilitate the entry of moisturizing factors into the skin, and achieve a significant effect of oil control and moisturization. Specific embodiments

[0041] To further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following, in combination with embodiments, details the specific embodiments, structures, features, and effects according to the present invention.

[0042] Example 1

[0043] An oil-control and moisturizing composition comprises the following components in parts by weight:

[0044]

[0045] The moisturizer is composed of betaine, glycerol, erythritol, and xylitol mixed in a mass ratio of 2: 5: 0.6: 0.8; the skin conditioner is ceramide; the chelating agent is disodium EDTA; the preservative is sodium sorbate; the pH regulator is disodium hydrogen phosphate.

[0046] The method for preparing the extract of mangosteen pericarp comprises the following steps: adding the powder of mangosteen pericarp into an ethanol solution and mixing, performing microwave extraction, taking the filtrate for vacuum concentration, and freeze-drying to obtain the extract of mangosteen pericarp; wherein, the dosage ratio of the powder of mangosteen pericarp and the ethanol solution is 120 mL: 5 g; the mass fraction of the ethanol solution is 60%; the microwave extraction is to microwave at 80 W for 2 min and then extract at 65 °C for 2 h; the temperature of the vacuum concentration is 50 °C.

[0047] The method for preparing the extract of white flower leaf comprises the following steps:

[0048] 1) Add an ethanol solution to the Brassica oleracea var. alboglabra leaf granules. After extracting multiple times, centrifuge and combine the supernatant to obtain the Brassica oleracea var. alboglabra leaf supernatant; among them, the dosage ratio of the Brassica oleracea var. alboglabra leaves to the ethanol solution is 1 g: 30 mL; extracting multiple times means extracting 2 times at 60 °C; the centrifugation conditions are centrifuging at a speed of 5000 r / min for 8 min.

[0049] 2) Concentrate the Brassica oleracea var. alboglabra leaf supernatant under reduced pressure to obtain the concentrated Brassica oleracea var. alboglabra leaf supernatant; among them, the reduced pressure concentration conditions are concentrating at 55 °C.

[0050] 3) Add ethanol to the concentrated Brassica oleracea var. alboglabra leaf supernatant, refrigerate at low temperature, and after centrifuging and discarding the supernatant, obtain the Brassica oleracea var. alboglabra leaf extract; among them, the volume ratio of the concentrated Brassica oleracea var. alboglabra leaf supernatant to ethanol is 1: 4.0; the ethanol is anhydrous ethanol, and the low-temperature refrigeration is refrigerating at 4 °C; the centrifugation conditions are centrifuging at 6000 r / min for 8 min.

[0051] Among them, the Brassica oleracea var. alboglabra leaf granules are obtained by drying, pulverizing, and sieving the Brassica oleracea var. alboglabra leaves through a 40-mesh sieve.

[0052] The preparation method of the Eryngium maritimum extract includes the following steps: Mix the Eryngium maritimum powder and the ethanol solution, heat under reflux, filter under reduced pressure, take the filtrate for rotary evaporation, dry, and cool to room temperature to obtain the Eryngium maritimum extract; among them, the dosage ratio of the Eryngium maritimum powder to the ethanol solution is 1 g: 30 mL; the mass fraction of the ethanol solution is 30%; the heating under reflux is at a temperature of 60 °C and reflux for 4 h.

[0053] A preparation method of an oil-control and moisture-retention composition includes the following steps:

[0054] S1. Heat deionized water to 75 °C, then add a moisturizer and a chelating agent and stir and mix at 600 r / min.

[0055] S2. Cool down to 60 °C, add carboxymethyl chitosan, a preservative, and a skin conditioner, and stir evenly.

[0056] S3. Cool down to 45 °C, add the Brassica oleracea var. alboglabra leaf extract, the mangosteen pericarp extract, the Eryngium maritimum extract, carboxymethyl chitosan, and a pH regulator, keep warm and stir at 35 °C, and filter to obtain the oil-control and moisture-retention composition.

[0057] Example 2

[0058] An oil-control and moisture-retention composition includes the following components by weight:

[0059]

[0060] The moisturizer is prepared by mixing betaine, glycerol, erythritol and xylitol in a mass ratio of 2:5:0.6:0.8; the skin conditioner is ceramide; the chelating agent is disodium EDTA; the preservative is sodium sorbate; the pH regulator is disodium hydrogen phosphate.

[0061] The preparation method of the mangosteen peel extract comprises the following steps: adding mangosteen peel powder into an ethanol solution, mixing, performing microwave extraction, taking the filtrate for reduced pressure concentration, and freeze-drying to obtain the mangosteen peel extract; wherein, the dosage ratio of the mangosteen peel powder to the ethanol solution is 123 mL:5 g; the mass fraction of the ethanol solution is 63%; the microwave extraction is to microwave at 93 W for 2.3 min and then extract at 67°C for 2.7 h; the temperature of the reduced pressure concentration is 50°C.

[0062] The preparation method of the white flower leaf extract comprises the following steps:

[0063] 1) Adding an ethanol solution to white flower leaf particles, extracting multiple times, centrifuging, and combining the supernatant liquids to obtain a white flower leaf supernatant liquid; wherein, the dosage ratio of the white flower leaf to the ethanol solution is 1 g:32 mL; the multiple extractions are to extract at 63°C for 2 times; the centrifuging conditions are to centrifuge at a rotational speed of 5300 r / min for 9 min;

[0064] 2) Performing reduced pressure concentration on the white flower leaf supernatant liquid to obtain a white flower leaf concentrated supernatant liquid; wherein, the reduced pressure concentration conditions are to concentrate at 57°C;

[0065] 3) Adding ethanol to the white flower leaf concentrated supernatant liquid, performing low-temperature refrigeration, centrifuging, and discarding the supernatant liquid to obtain a white flower leaf extract; wherein, the volume ratio of the white flower leaf concentrated supernatant liquid to the ethanol is 1:4.2; the ethanol is anhydrous ethanol, the low-temperature refrigeration is to refrigerate at 4°C; the centrifuging conditions are to centrifuge at 6300 r / min for 9 min.

[0066] Among them, the white flower leaf particles are obtained by drying, pulverizing, and sieving the white flower leaves through a 40-mesh sieve;

[0067] The preparation method of the Eryngium maritimum extract comprises the following steps: mixing Eryngium maritimum powder and an ethanol solution, heating under reflux, performing reduced pressure filtration, taking the filtrate for rotary evaporation, drying, and cooling to room temperature to obtain the Eryngium maritimum extract; wherein, the dosage ratio of the Eryngium maritimum powder to the ethanol solution is 1 g:33 mL; the mass fraction of the ethanol solution is 33%; the heating under reflux is at a temperature of 63°C and a reflux time of 4.7 h.

[0068] A preparation method of an oil-control and moisturizing composition comprises the following steps:

[0069] S1. After heating deionized water to 78°C, add a humectant and a chelating agent, and stir and mix at 660 r / min;

[0070] S2. Cool down to 62°C, add carboxymethyl chitosan, a preservative, and a skin conditioner, and stir evenly;

[0071] S3. Cool down to 47°C, add white flower leaf extract, mangosteen peel extract, eryngium maritimum extract, carboxymethyl chitosan, and a pH regulator, keep warm and stir at 38°C, and filter to obtain an oil-control and moisture-retaining composition.

[0072] Example 3

[0073] An oil-control and moisture-retaining composition comprises the following components by weight:

[0074]

[0075] The humectant is composed of betaine, glycerol, erythritol, and xylitol mixed in a mass ratio of 2:5:0.6:0.8; the skin conditioner is ceramide; the chelating agent is disodium EDTA; the preservative is sodium sorbate; the pH regulator is disodium hydrogen phosphate.

[0076] The preparation method of the mangosteen peel extract comprises the following steps: Add mangosteen peel powder to an ethanol solution, mix, perform microwave extraction, take the filtrate, perform vacuum concentration, and perform freeze-drying to obtain the mangosteen peel extract; wherein, the dosage ratio of the mangosteen peel powder to the ethanol solution is 127 mL:5 g; the mass fraction of the ethanol solution is 67%; the microwave extraction is to microwave at 107 W for 2.7 min and then extract at 68°C for 3.3 h; the temperature of the vacuum concentration is 50°C.

[0077] The preparation method of the white flower leaf extract comprises the following steps:

[0078] 1) Add an ethanol solution to white flower leaf particles, perform multiple extractions, centrifuge, and combine the supernatant to obtain a white flower leaf supernatant; wherein, the dosage ratio of the white flower leaf to the ethanol solution is 1 g:33 mL; the multiple extractions are to extract 3 times at 67°C; the centrifugation condition is to centrifuge at a rotation speed of 5600 r / min for 11 min;

[0079] 2) Perform vacuum concentration on the white flower leaf supernatant to obtain a white flower leaf concentrated supernatant; wherein, the vacuum concentration condition is to concentrate at 58°C;

[0080] 3) Ethanol is added to the concentrated supernatant of Brassica rapa var. chinensis leaf, followed by cold storage at low temperature. After centrifugation, the supernatant is discarded to obtain the Brassica rapa var. chinensis leaf extract. Among them, the volume ratio of the concentrated supernatant of Brassica rapa var. chinensis leaf to ethanol is 1:4.3; the ethanol is anhydrous ethanol, and the low-temperature refrigeration is refrigeration at 4°C; the centrifugation conditions are centrifugation at 6600 r / min for 11 min.

[0081] Among them, the Brassica rapa var. chinensis leaf particles are obtained by drying, pulverizing, and sieving the Brassica rapa var. chinensis leaves through a 40-mesh sieve.

[0082] The preparation method of the Eryngium maritimum extract includes the following steps: After mixing the Eryngium maritimum powder and the ethanol solution, heat under reflux, perform vacuum filtration, take the filtrate for rotary evaporation, dry, and cool to room temperature to obtain the Eryngium maritimum extract. Among them, the dosage ratio of the Eryngium maritimum powder to the ethanol solution is 1 g:37 mL; the mass fraction of the ethanol solution is 37%; the heat reflux is at a temperature of 67°C and a reflux time of 5.3 h.

[0083] A preparation method of an oil-control and moisturizing composition includes the following steps:

[0084] S1. After heating deionized water to 82°C, add a moisturizer and a chelating agent and stir and mix at 730 r / min;

[0085] S2. Cool down to 63°C, add carboxymethyl chitosan, a preservative, and a skin conditioner, and stir evenly;

[0086] S3. Cool down to 48°C, add the Brassica rapa var. chinensis leaf extract, the mangosteen pericarp extract, the Eryngium maritimum extract, carboxymethyl chitosan, and a pH regulator, keep stirring at 42°C, and filter to obtain the oil-control and moisturizing composition.

[0087] Example 4

[0088] An oil-control and moisturizing composition includes the following components by weight:

[0089]

[0090] The moisturizer is composed of betaine, glycerol, erythritol, and xylitol mixed in a mass ratio of 2:5:0.6:0.8; the skin conditioner is ceramide; the chelating agent is disodium EDTA; the preservative is sodium sorbate; the pH regulator is disodium hydrogen phosphate.

[0091] The preparation method of the mangosteen pericarp extract comprises the following steps: adding mangosteen pericarp powder into an ethanol solution, mixing, performing microwave extraction, taking the filtrate for reduced pressure concentration, and performing freeze drying to obtain the mangosteen pericarp extract; wherein, the dosage ratio of the mangosteen pericarp powder to the ethanol solution is 130 mL: 5 g; the mass fraction of the ethanol solution is 70%; the microwave extraction is performed by microwaving at 120 W for 3 min and then extracting at 70°C for 4 h; the temperature of the reduced pressure concentration is 50°C.

[0092] The preparation method of the extract of Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaves comprises the following steps:

[0093] 1) Adding an ethanol solution to the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf particles, performing multiple extractions, centrifuging, and combining the supernatant liquids to obtain the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf supernatant liquid; wherein, the dosage ratio of the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaves to the ethanol solution is 1 g: 35 mL; the multiple extractions are performed by extracting at 70°C for 3 times; the centrifuging conditions are centrifuging at a rotation speed of 6000 r / min for 12 min;

[0094] 2) Performing reduced pressure concentration on the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf supernatant liquid to obtain the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf concentrated supernatant liquid; wherein, the reduced pressure concentration conditions are concentrating at 60°C;

[0095] 3) Adding ethanol to the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf concentrated supernatant liquid, performing low-temperature refrigeration, centrifuging, and discarding the supernatant liquid to obtain the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf extract; wherein, the volume ratio of the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf concentrated supernatant liquid to the ethanol is 1: 4.5; the ethanol is anhydrous ethanol, the low-temperature refrigeration is refrigerating at 4°C; the centrifuging conditions are centrifuging at 7000 r / min for 12 min.

[0096] Among them, the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaf particles are obtained by drying, pulverizing, and sieving the Brassicacampestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee leaves through a 40-mesh sieve;

[0097] The preparation method of the Eryngium maritimum extract comprises the following steps: mixing Eryngium maritimum powder and an ethanol solution, performing heating under reflux, performing reduced pressure suction filtration, taking the filtrate for rotary evaporation, drying, and cooling to room temperature to obtain the Eryngium maritimum extract; wherein, the dosage ratio of the Eryngium maritimum powder to the ethanol solution is 1 g: 40 mL; the mass fraction of the ethanol solution is 40%; the heating under reflux is at a temperature of 70°C and a reflux time of 6 h.

[0098] A preparation method of an oil-control and moisture-retention composition comprises the following steps:

[0099] S1. Heating deionized water to 85°C, adding a moisturizing agent and a chelating agent, and stirring and mixing at 800 r / min;

[0100] S2. Cooling to 65°C, adding carboxymethyl chitosan, a preservative, and a skin conditioner, and stirring evenly;

[0101] S3. Cool down to 50°C, add white flower leaf extract, mangosteen peel extract, Eryngium maritimum extract, carboxymethyl chitosan and a pH regulator, keep warm and stir at 45°C, then filter to obtain the oil-control and moisture-retention composition.

[0102] Comparative Examples 1-4

[0103] Compared with Example 3, the difference lies in that the weight ratios of the white flower leaf extract, mangosteen peel extract, Eryngium maritimum extract and carboxymethyl chitosan are as shown in Table 1, and the other components, preparation steps and parameters are the same.

[0104] Table 1

[0105]

[0106] Test Example 1

[0107] Antioxidant test:

[0108] 1. Experiment on scavenging DPPH free radicals

[0109] Samples to be tested: The oil-control and moisture-retention compositions prepared in Examples 1-4 and Comparative Examples 1-4;

[0110] In a 10 mL colorimetric tube, add 4.0 mL of DPPH solution and the sample to be tested in sequence, then add absolute ethanol to the scale line and mix well. Immediately use a 1 cm cuvette to measure the absorbance at a wavelength of 517 nm and record it as Ai. Then, after standing at room temperature in the dark for 30 minutes, measure the absorbance again and record it as Aj. At the same time, set up a blank control group, only add DPPH ethanol solution, measure its absorbance value and record it as Ac. Calculate the DPPH free radical scavenging rate K(%) according to the following formula:

[0111] K = [1 - (Ai - Aj) / Ac] * 100%;

[0112] The experiment was repeated 3 times and the average value was taken. The test results are shown in Table 2.

[0113] 2. Experiment on evaluating the antioxidant efficacy of cells

[0114] First, prepare sample solutions (Examples 1-4, Comparative Examples 1-4) with a concentration of 0.5% in serum-free DMEM culture medium respectively, filter and sterilize with a 0.22 μm filter membrane, and then prepare a dichlorofluorescein diacetate (DCFH-DA) probe solution: dilute according to the ratio of

[0115] 0.375 μL DCFH-DA: 1 mL PBS. Take HaCaT cells in the logarithmic growth phase, at a density of 1×10 5Inoculate at a density of cells / mL into a 12-well culture plate. Select 10 of them, with 2 mL of cell suspension in each well, and place them in a carbon dioxide incubator for routine culture at 37 °C and 5% CO2 for 24 h. For the model group and the experimental group, irradiate with UVA at an intensity of 2000 μW / cm 2 for 1 h, and irradiate with UVB at an intensity of 700 μW / cm 2 for 3 min. The normal control group is not irradiated. After irradiation, remove the old culture medium, add the sample solution to the experimental group, and add serum-free DMEM culture medium to the model group and the normal control group, 2 mL in each well. After continuing to culture for 16 hours, remove all the culture medium, wash twice with PBS, add 1.5 mL of DCFH-DA solution to each well, and place it in a 37 °C cell culture incubator for incubation for 30 minutes, mixing once every 5 minutes during this period to ensure sufficient binding of the probe; remove the probe, wash twice with pre-warmed serum-free medium, add 1 mL of serum-free medium to each well, and incubate at 37 °C for 10 minutes; after washing once with PBS, digest the cells with trypsin, then wash twice with PBS, and resuspend with 300 μL of PBS; perform dual-channel detection by flow cytometry (filter the cells through a 100-mesh filter before loading), use the FL1-H channel, and collect 10,000 cells for each sample.

[0116] ROS scavenging rate = (model group - experimental group) / model group × 100%;

[0117] The experimental results of each sample are shown in Table 2.

[0118] Table 2

[0119]

[0120]

[0121] It can be seen from the test results in Table 2 that compared with Comparative Examples 1-4, the oil-control and moisture-retention compositions prepared by the inventive scheme have significantly better DPPH scavenging rate and ROS scavenging rate than Comparative Documents 1-4.

[0122] Test Example 2

[0123] A total of 80 volunteers with oily or combination skin aged between 25 and 35 years, with healthy and undamaged skin, were selected, including 40 males and 40 females. Subsequently, these 80 volunteers were randomly and evenly divided into 8 groups. Each group used the oil-control and moisture-retention compositions prepared in Examples 1-4 and Comparative Examples 1-4 respectively. During the experiment, the forehead area of the volunteers' faces was used as the test area for applying the samples, and the usage frequency was once in the morning and once in the evening every day for 28 days.

[0124] On the 0th day and the 28th day of using the product, the skin surface oil content of the forehead area was detected 3 times using an oil measuring instrument, and the average value was taken. By comparing the oil content at these two time points, the reduction in the skin surface oil content was calculated.

[0125] The results are shown in Table 3.

[0126] Table 3

[0127]

[0128]

[0129] As can be seen from Table 3, by reducing the level of lipid peroxidation, the oil-control and moisturizing compositions of Examples 1-4 of the present invention can effectively reduce the oil secretion on the skin surface. While for the oil-control and moisturizing compositions of Comparative Examples 1-4, the reduction in the oil secretion on the skin surface is relatively low.

[0130] Test Example 3

[0131] Test samples: The oil-control and moisturizing compositions prepared from Examples 1-4 and Comparative Examples 1-4.

[0132] Participant selection: Eighty volunteers aged between 30 and 45 years with skin dryness problems were selected, including 40 males and 40 females. These volunteers were randomly assigned to 8 groups, with 10 volunteers in each group. The facial area of the volunteers was used as the test area, and the corresponding test samples were applied.

[0133] Usage method and period: The volunteers were required to apply the samples once on the facial area every morning and evening for 28 consecutive days.

[0134] Evaluation method: On the 0th day and the 28th day of using the product, the skin moisture content of the volunteers was detected using a skin moisture content tester Corneometer CM825. By comparing the data before and after using the samples, the oil-control and moisturizing effects of each sample were evaluated. The results are shown in Table 4.

[0135] Table 4

[0136]

[0137]

[0138] As can be seen from Table 4, the oil-control and moisturizing compositions of Examples 1-4 of the present invention can effectively lock in the moisture on the skin surface. Combining with the action of the moisturizing factors, it can effectively prevent the evaporation of skin moisture, thus achieving the moisturizing effect. While for the oil-control and moisturizing compositions of Comparative Examples 1-4, the growth rate of the skin surface moisture content is relatively low.

[0139] The above are only the preferred embodiments of the present invention and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above with the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. An oil-control and moisture-retention composition, characterized in that, It is composed of the following components by weight parts: White flower leaf extract 2 - 4 parts Mangosteen peel extract 3.0 - 4.5 parts Eryngium maritimum extract 0.8 - 1.6 parts Carboxymethyl chitosan 0.5 - 0.8 parts Humectant 15 - 24 parts Skin conditioner 0.1 - 0.2 parts Chelating agent 0.1 - 0.3 parts Preservative 0.2 - 0.3 parts pH regulator 0.1 - 0.3 parts Deionized water 50 - 60 parts.

2. The oil-control and moisture-retaining composition according to claim 1, wherein: The humectant is one or more of betaine, glycerol, erythritol and xylitol.

3. The oil-control and moisture-retaining composition according to claim 1, wherein: The humectant is composed of betaine, glycerol, erythritol and xylitol mixed in a mass ratio of 2:5:0.6:0.

8.

4. The oil-control and moisture-retention composition according to claim 1, wherein: The skin conditioner is one or both of ceramide and cholesterol.

5. The oil-control and moisture-retaining composition according to claim 1, wherein: The chelating agent is disodium EDTA; the preservative is one or more of sorbic acid, sodium sorbate and potassium sorbate.

6. The oil-control and moisture-retaining composition according to claim 1, wherein: The pH regulator is one or more of disodium hydrogen phosphate, citric acid and sodium citrate.

7. A method for preparing an oil-control and moisturizing composition according to any one of claims 1-6, characterized in that, The preparation method includes the following steps: S1. After heating deionized water to 75 - 85 °C, add the humectant and chelating agent and stir and mix at 600 - 800 r / min; S2. Cool down to 60 - 65 °C, add carboxymethyl chitosan, preservative and skin conditioner, and stir evenly; S3. Cool down to 45 - 50 °C, add white flower leaf extract, mangosteen peel extract, eryngium maritimum extract and pH regulator, keep warm and stir at 35 - 45 °C, filter to obtain the oil-control and moisture-retention composition.

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