A barley sprout growing method that is easy to powder process and has little sediment after the product is brewed

Abstract

A method for cultivating barley seedlings that is easy to process into powder and produces less sediment after brewing involves directly cultivating barley seeds in soil under high temperature, high humidity, and high fertilizer conditions after germination. The resulting barley seedlings have shorter cellulose chains. After freeze-drying and pulverizing, the content of insoluble cellulose in the barley seedling powder is greatly reduced, resulting in significantly less sediment after brewing with water, thus improving the product's taste and increasing its competitiveness.

Description

Technical Field

[0001] This invention belongs to the field of agricultural product planting technology, specifically relating to a method for planting barley seedlings that is easy to process into powder and produces little sediment after brewing. Background Technology

[0002] Barley grass powder is made from the tender leaves of high-quality organic barley seedlings that have just overwintered and grown to 25-40 cm in height, using advanced freeze-drying and pulverizing technology. It is rich in dietary fiber, vitamins, and trace elements, making it a pure natural health food.

[0003] However, it has been found that barley grass powder produces a lot of sediment after brewing. There is currently no effective method to fundamentally reduce the sediment residue left after brewing barley grass powder. Summary of the Invention

[0004] The purpose of this invention is to provide a barley seedling cultivation method that is easy to process into powder and produces less sediment after brewing. This method effectively reduces the cellulose chain length in barley seedlings, thereby significantly reducing sedimentation in barley seedling powder prepared from barley seedlings after brewing.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A method for cultivating barley seedlings that is easy to process into powder and produces little sediment after brewing includes the following steps:

[0007] 1) Seed pretreatment

[0008] Disinfect plump, disease-free barley seeds, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts.

[0009] 2) Soil cultivation treatment

[0010] After germination, the seeds were transferred to soil that was evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 25–35 days to obtain barley seedlings. The cultivation conditions were: temperature 22–32℃, humidity 50–85%, light 13–17 h / d, and light intensity 5000–7000 Lux.

[0011] The compound short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitosan oligosaccharide, and gibberellin. The mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and water is 0.1–2:20–60:20–50:0–0.1:50–150. The concentration of chitosan oligosaccharide is 0–200 mg / L, and the concentration of gibberellin is 10 mg / L. -10 ~10 -7 mol / L.

[0012] Preferably, in step 1), the barley seeds are disinfected with a disinfectant solution at a concentration of 5-10%.

[0013] Preferably, in step 1), the seeds show white sprouts 22-26 hours after germination.

[0014] A barley seedling powder is prepared from barley seedlings obtained by the above-mentioned planting method.

[0015] This invention allows for direct soil cultivation after seed germination, eliminating the need for a low-temperature vernalization process. Seeds that have sprouted are directly cultivated in soil at high temperatures, with controlled temperatures of 22–32°C and humidity of 50–85%. Compound short-chain fertilizer is also added. Under the high-temperature, high-humidity, and high-fertilizer growing environment, the germinated seeds do not form sufficient cellulose in the plant because they did not undergo low-temperature vernalization in the early stages. As a result, the plants grow faster, are greener, and have shorter cellulose chains during the later soil cultivation process.

[0016] In this invention, the combination of high temperature, high humidity, high fertilizer, long light duration, appropriate growth hormone and nutrients that improve hormone tolerance greatly promotes the growth rate of seedlings, shortens the seedling cultivation time, reduces the time for cellulose to be generated in the seedlings, thereby reducing the proportion of cellulose and the length of fiber chains, thus reducing the phenomenon of sedimentation after product brewing.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] This invention involves directly cultivating barley seeds in soil under high temperature, high humidity, and high fertilizer conditions after germination. Compared with conventional cultivation techniques, this method eliminates the vernalization process, shortens the seedling cultivation time, promotes seedling growth, and reduces the time for cellulose to be generated in the seedlings, thereby reducing the proportion of cellulose and the length of fiber chains.

[0019] The barley seedlings obtained using the planting technology of this invention are freeze-dried and pulverized to obtain barley seedling powder. Because the cellulose chain length in barley seedlings is relatively short, the content of insoluble cellulose in barley seedling powder is greatly reduced. After being brewed with water, sedimentation is significantly reduced, improving the taste of the product and increasing its competitiveness.

[0020] Currently, there are almost no such technologies. Normal technologies focus on improving lodging resistance (increasing cellulose content and chain length) and the main technical direction is to improve seed quality. There are very few reverse interventions during the seedling stage. Detailed Implementation

[0021] The present invention will be further described below with reference to specific embodiments.

[0022] In the following examples, the barley seed variety used was “H30”, which was cultivated by the Institute of Biotechnology, Shanghai Academy of Agricultural Sciences; the chitosan, ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and gibberellin used were all of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd.

[0023] Example 1: A method for cultivating cereal crops that is easy to process into powder and produces little sediment after brewing, comprising the following steps:

[0024] 1) Seed pretreatment

[0025] Disinfect plump, disease-free barley seeds with a 5% disinfectant solution, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts.

[0026] 2) Soil cultivation treatment

[0027] After germination, the seeds were transferred to soil that was evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 35 days to obtain barley seedlings. The cultivation conditions were: temperature 32℃, humidity 85%, light 15h / d, and light intensity 5000Lux.

[0028] The compound short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitosan oligosaccharide, and gibberellin. The mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and water is 0.1:20:30:0.1:50. The concentration of chitosan oligosaccharide is 200 mg / L, and the concentration of gibberellin is 10 mg / L. -10 mol / L.

[0029] The obtained barley grass was freeze-dried and pulverized to obtain barley grass powder. The amount of insoluble cellulose, the molecular weight of cellulose, and the cellulose content were measured.

[0030] Example 2: A method for cultivating cereal crops that is easy to process into powder and produces little sediment after brewing, comprising the following steps:

[0031] 1) Seed pretreatment

[0032] Disinfect plump, disease-free barley seeds with a 10% disinfectant solution, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts.

[0033] 2) Soil cultivation treatment

[0034] After germination, the seeds were transferred to soil that was evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 25 days to obtain barley seedlings. The cultivation conditions were: temperature 28℃, humidity 65%, light 13h / d, and light intensity 6000Lux.

[0035] The compound short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitosan oligosaccharide, and gibberellin. The mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and water is 1:60:20:0.05:100, the concentration of chitosan oligosaccharide is 100 mg / L, and the concentration of gibberellin is 10 mg / L. -7 mol / L.

[0036] The obtained barley grass was freeze-dried and pulverized to obtain barley grass powder. The amount of insoluble cellulose, the molecular weight of cellulose, and the cellulose content were measured.

[0037] Example 3: A method for cultivating cereal crops that is easy to process into powder and produces little sediment after brewing, comprising the following steps:

[0038] 1) Seed pretreatment

[0039] Disinfect plump, disease-free barley seeds with a 7% disinfectant solution, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts.

[0040] 2) Soil cultivation treatment

[0041] After germination, the seeds were transferred to soil that was evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 30 days to obtain barley seedlings. The cultivation conditions were: temperature 22℃, humidity 50%, light 17h / d, and light intensity 7000Lux.

[0042] The compound short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitosan oligosaccharide, and gibberellin. The mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and water is 2:60:40:0.01:150. The concentration of chitosan oligosaccharide is 10 mg / L, and the concentration of gibberellin is 10 mg / L. -8 mol / L.

[0043] The obtained barley grass was freeze-dried and pulverized to obtain barley grass powder. The amount of insoluble cellulose, the molecular weight of cellulose, and the cellulose content were measured.

[0044] Example 4: A method for cultivating cereal crops that is easy to process into powder and produces little sediment after brewing, comprising the following steps:

[0045] 1) Seed pretreatment

[0046] Disinfect plump, disease-free barley seeds with a 7% disinfectant solution, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts.

[0047] 2) Soil cultivation treatment

[0048] After germination, the seeds were transferred to soil that was evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 30 days to obtain barley seedlings. The cultivation conditions were: temperature 25℃, humidity 70%, light 16h / d, and light intensity 7000Lux.

[0049] The compound short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitosan oligosaccharide, and gibberellin. The mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, and water is 2:60:40:0.01:150. The concentration of chitosan oligosaccharide is 10 mg / L, and the concentration of gibberellin is 10 mg / L. -8 mol / L.

[0050] The obtained barley grass was freeze-dried and pulverized to obtain barley grass powder. The amount of insoluble cellulose, the molecular weight of cellulose, and the cellulose content were measured.

[0051] The comparison is based on existing products, specifically barley grass powder already available on the market.

[0052] The present invention detects the lignin content, cell wall cellulose content, and cell wall extract content in the barley grass powder obtained in the examples and comparative examples, respectively.

[0053] 1. Cell wall extraction method:

[0054] 1.1: Weigh out an appropriate amount of dried plant powder;

[0055] 1.2: Add 1 mL of 70% ethanol, vortex thoroughly, centrifuge at 10000 rpm for 10 min, and discard the supernatant;

[0056] 1.3: Add 1 mL of chloroform / methanol (1:1 v / v) solution, vortex thoroughly, centrifuge at 10000 rpm for 10 min, and discard the supernatant after centrifugation;

[0057] 1.4: Add 1 mL of acetone, vortex thoroughly, and then vacuum dry the acetone.

[0058] 1.5: Add 1.5 mL of 0.1 M pH 5.0 sodium acetate buffer and heat at 80 °C for 20 min;

[0059] 1.6: Add 10 μL of 0.01% sodium azide, amylase, and pullulanase, and incubate overnight at 37°C;

[0060] 1.7: The reaction was terminated by heating at 100℃, centrifuged at 10000rpm for 10min, the supernatant was discarded, and the precipitate was washed with distilled water;

[0061] 1.8: Add 1 mL of acetone, vortex thoroughly, and then vacuum dry the acetone.

[0062] 1.9: The residue was dried at 40°C to obtain cell wall material.

[0063] 2. Detection of cell wall lignin content

[0064] This invention utilizes a Thermo Fisher Scientific (USA) multi-functional microplate reader (Multiskan GO) for quantitative analysis of target analytes. Corning (USA) microplates are used, requiring a well-to-well difference of less than 0.002 at the target wavelength.

[0065] Weigh 2 mg of the cell wall material from the embodiments and comparative examples of the present invention, add 100 μL of acetyl bromide (25%) solution, and incubate at 37°C for 6 h; then add 100 μL of 2M sodium hydroxide and 100 μL of 0.5M hydroxylamine hydrochloride, vortex to mix, and bring the volume to 2 mL with acetic acid; after centrifugation at 10000 rpm for 10 min, take the supernatant and measure the absorbance at 280 nm.

[0066]

[0067] In the formula: ABS is the absorbance value; Coeff is the absorption coefficient; Weight is the mass of the cell wall material in mg; 0.539 cm is the optical path length.

[0068] Note: Multiply the percentage lignin content value by 10 to get the lignin content (μg / mg cell wall material).

[0069] 3. Cell wall cellulose content detection

[0070] 3.1: Weigh 2 mg of cell wall material into a centrifuge tube;

[0071] 3.2: Add 0.5 mL of 2 M TFA, react at 100 °C for 6 h, centrifuge, discard the supernatant, and dry under nitrogen.

[0072] 3.3: Add 0.5 mL of isopropanol to the centrifuge tube, vortex to mix, evaporate to dryness, and repeat this step 3 times;

[0073] 3.4: Add 0.3 mL of 75% sulfuric acid to the tube, mix well, let stand at room temperature for 1 hour, and then add distilled water to make up to 1 mL.

[0074] 3.5: Centrifuge at 10000 rpm for 10 min, take 100 μL of supernatant, and add 200 μL of 2 mg / mL anthrone.

[0075] 3.6: React at 880℃ for 30 min, and measure the absorbance at 625 nm.

[0076] The cell wall cellulose content in the sample can be calculated based on the standard curve:

[0077] Cell wall cellulose content (μg / mg cell wall material) = C × (V ÷ V1) × D ÷ M;

[0078] In the formula: C is the result calculated based on the standard curve; M is the actual weighed mass; V is the total volume; V1 is the volume used in the reaction; and D is the dilution factor.

[0079] The percentage content of cell wall cellulose (μg / mg cell wall material) divided by 10 is the mass percentage content.

[0080] The results of the embodiments and comparative measurements of the present invention are shown in Table 1.

[0081] Table 1

[0082]

[0083]

[0084] As can be seen from Table 1, the cell wall extract, lignin content, and cellulose content in the barley grass powder obtained in the embodiments of the present invention are significantly lower than those in the comparative example.

Claims

1. A method for cultivating barley seedlings that is easy to process into powder and produces little sediment after brewing, comprising the following steps: 1) Seed pretreatment Disinfect plump, disease-free barley seeds, rinse them clean, wrap them in a damp cloth, and place them in a sealed plastic container to germinate until the seeds show white sprouts. 2) Soil cultivation treatment After germination, the seeds were transferred to soil evenly mixed with compound short-chain fertilizer and cultivated in an artificial climate chamber for 25–35 days to obtain barley seedlings. The cultivation conditions were: temperature 28–32℃, humidity 85%, light 13–17 h / d, and light intensity 5000–7000 Lux. The composite short-chain fertilizer is composed of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate, water, chitooligosaccharide and gibberellin, wherein the mass ratio of ammonium nitrate, potassium nitrate, calcium nitrate, sodium molybdate and water is 0.1-2:20-60:20-50:0-0.1:50-150, the concentration of chitooligosaccharide is 10-200 mg / L, and the concentration of gibberellin is 10 -10 -10 -7 mol / L. It reduced the content of cell wall lignin and cell wall cellulose in barley seedlings.

2. The barley seedling planting method according to claim 1, characterized in that, In step 1), the barley seeds are disinfected with a disinfectant solution at a concentration of 5-10%.

3. The barley seedling planting method according to claim 1, characterized in that, In step 1), the seeds will show white sprouts 22-26 hours after germination.

4. A barley grass powder, characterized in that, The barley seedlings are prepared by the barley seedling planting method according to any one of claims 1 to 3.

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