Gastrodia bag breeding method

Abstract

This invention discloses a method for bagged breeding of Gastrodia elata, belonging to the field of Gastrodia elata cultivation technology. The method includes steps such as breeding substrate preparation, seed mixing, and breeding. This invention uses coconut coir as the main raw material to prepare the breeding substrate for Gastrodia elata cultivation and breeding. The prepared breeding substrate can better meet the nutrient, humidity, temperature, and pH conditions required for Gastrodia elata growth, and better promote the germination of Gastrodia elata seeds and increase the yield of Gastrodia elata. It solves the problems of poor moisture retention and easy compression of Gastrodia elata when using sandy soil as a substrate in conventional breeding methods, which leads to a decrease in the yield and quality of Gastrodia elata. At the same time, it can reduce labor input costs and has good application prospects in the field of Gastrodia elata cultivation technology.

Description

Technical Field

[0001] This invention relates to the field of Gastrodia elata cultivation technology, and in particular to a method for bagged breeding of Gastrodia elata. Background Technology

[0002] Gastrodia elata, a perennial edible fungus herb belonging to the genus Gastrodia in the Orchidaceae family, has been used medicinally in my country for over 2000 years and is a traditional and precious Chinese medicinal herb. Its tuber is used medicinally, and its main active ingredients are gastrodin and gastrodin aglycone. With the deepening of research on Gastrodia elata, new active ingredients and pharmacological effects are constantly being discovered, leading to a continuous increase in demand. Especially in recent years, the rapid advancement of the work on the homology of Gastrodia elata as both food and medicine has significantly boosted market demand. Therefore, large-scale, standardized, and efficient cultivation of Gastrodia elata has become inevitable.

[0003] Because the plastic bag cultivation method for Gastrodia elata can make better use of space and save costs, it can solve the problems of large propagation sites required for field sowing, susceptibility to environmental factors such as temperature and humidity, and difficulty in management, and is therefore widely used. In traditional bag cultivation methods, a mixture of sandy soil and sawdust is often used as a substrate for the breeding and cultivation of Gastrodia elata. However, the poor moisture retention of sandy soil and sawdust mixture can lead to the failure of Gastrodia elata seeds to germinate, resulting in empty bags. After the seeds germinate, the large mass of the sandy soil causes the Gastrodia elata seeds to be squeezed during growth, resulting in low yield and uneven seed size. Moreover, the bags filled with sandy soil are heavy, making stacking laborious and time-consuming. Summary of the Invention

[0004] Based on the above-mentioned technical problems, the purpose of this invention is to provide a method for bag-based breeding of Gastrodia elata, which solves the problems of insufficient moisture retention of the substrate in traditional bag-based cultivation, resulting in seed non-germination, and the large weight of the substrate causing compression of Gastrodia elata, thus reducing the yield and quality of Gastrodia elata.

[0005] The present invention solves the above-mentioned technical problems through the following technical means:

[0006] A method for breeding Gastrodia elata in bags, the method is as follows:

[0007] (1) Preparation of breeding substrate: Breeding substrate was prepared using coconut soil as raw material;

[0008] (2) Seed dressing: Germination fungus and Gastrodia elata seeds are mixed evenly to prepare germination fungus-seed mixture;

[0009] (3) Breeding: Using plastic bags as breeding bags, first spread the breeding substrate evenly in the breeding bag, then evenly sprinkle a layer of germination fungus-seed mixture, then place the fungus sticks and Armillaria mellea, and repeat the operation of spreading the breeding substrate, sprinkling the germination fungus-seed mixture and placing the fungus sticks and Armillaria mellea twice. Finally, after spreading a layer of breeding substrate, tie the breeding bag and place it in a shaded area under the forest for breeding.

[0010] Furthermore, the breeding substrate includes the following raw materials:

[0011] The breeding substrate includes the following raw materials:

[0012] Coconut clay, tert-butylethanolamine, diethylene glycol dimethyl ether, corn steep liquor, wheat bran, ammonium dihydrogen phosphate, magnesium sulfate.

[0013] Furthermore, the method for preparing the breeding substrate is as follows:

[0014] A: Dissolve tert-butylethanolamine in water and add it to coconut fiber. Heat the mixture to 60-80℃ and stir for 4-6 hours. After the reaction is complete, sonicate for 30-60 minutes. Then add diethylene glycol dimethyl ether, stir and mix evenly. Let stand for 1-2 days, filter to remove the filtrate to obtain the treated coconut fiber. Let it air dry under natural conditions for later use.

[0015] B: Dissolve ammonium dihydrogen phosphate and magnesium sulfate in water and add them to the dried treated coconut soil. Then add corn syrup and wheat bran and stir to mix evenly. Adjust the moisture content and pH to obtain the breeding substrate.

[0016] This invention uses coconut coir as a breeding substrate for the breeding and cultivation of Gastrodia elata. Coconut coir is lightweight, soft, and has good heat retention properties. When used for Gastrodia elata cultivation, it can better meet the humidity and temperature requirements for Gastrodia elata growth, and is less likely to cause compression of Gastrodia elata, thus improving the yield and quality of Gastrodia elata. In addition, using coconut coir as a substrate for the breeding and cultivation of Gastrodia elata can greatly reduce the weight of the bags and reduce the labor input costs during handling and stacking.

[0017] However, when cultivating Gastrodia elata in bags, the water in the breeding substrate, which is mainly composed of coconut coir, tends to flow from the upper layer to the lower layer due to gravity. This results in insufficient water content in the upper layer and excessive water content in the lower layer. Insufficient water content is not conducive to the growth of Gastrodia elata, while excessive water can cause Armillaria mellea to digest the Gastrodia elata, leading to empty shells and rotten Gastrodia elata. Therefore, it is necessary to treat the coconut coir substrate to inhibit the downward loss of water and maintain the relative stability of the water content in the upper and lower layers of the substrate.

[0018] Specifically, this invention first heats and reacts coconut fiber with tert-butylethanolamine, then ultrasonically treats it. Next, diethylene glycol dimethyl ether is added for further treatment to obtain treated coconut fiber. The reaction of tert-butylethanolamine with coconut fiber disrupts the intramolecular and intermolecular hydrogen bonds, thereby destroying the ordered structure of the coconut fiber and exposing a large number of hydrophilic hydroxyl functional groups within the fiber. Further, diethylene glycol dimethyl ether is grafted onto the cellulose molecular chain, reducing the intermolecular forces between the coconut fiber molecular chains and thus improving the water absorption and swelling properties of the coconut fiber. After adding water to adjust the substrate humidity, water molecules in the substrate can more firmly bind with the large number of exposed hydrophilic hydroxyl groups and be stored in the expanded fiber chain structure, thereby reducing the downward permeation of water in the coconut fiber substrate, ensuring the relative stability of the moisture content in the substrate, promoting better growth of Gastrodia elata seeds, and reducing the occurrence of empty shells and rotten seeds. Furthermore, the reduced structural order of the coconut fiber makes it easier to decompose and release nutrients, thus ensuring a comprehensive supply of nutrients in the substrate when combined with corn steep liquor, bran, and other raw materials.

[0019] Furthermore, in step A, the mass ratio of coconut fiber to tert-butylethanolamine and diethylene glycol dimethyl ether is (30-50):(0.4-0.6):(1-2).

[0020] Furthermore, in step B, the mass ratio of ammonium dihydrogen phosphate, magnesium sulfate, treated coconut coir, corn steep liquor, and wheat bran is (0.4-0.6):(0.2-0.4):(30-50):(2-4):(1-2).

[0021] Furthermore, in step B, the pH is adjusted to 5.5-6, and the water content is adjusted to 50-60%.

[0022] Furthermore, the mixing operation in step (2) is as follows:

[0023] Select germination bacteria with good growth and high germination rate. Decompose the germination bacteria into particles of 1-2 cm in size. Gently shake out the seeds from the capsules of Gastrodia elata and sprinkle them on the decomposed germination bacteria while sprinkling to mix the seeds and germination bacteria evenly. Mix 10-16 capsules of Gastrodia elata per kilogram of germination bacteria. After mixing evenly, let it stand at room temperature for 1-5 days to obtain the germination bacteria-seed mixture, which can then be used for Gastrodia elata breeding.

[0024] Furthermore, the substrate used for the mushroom substrate is any one of the following: Oak, Quercus acutissima, Quercus variabilis, and Quercus cuspidata.

[0025] Furthermore, the mushroom sticks are 5-10cm in diameter and 15-25cm in length, and are cut with 2-3 rows of fish-scale-like cuts that reach the xylem. The mushroom sticks are soaked in clean water for 2-3 days before use.

[0026] Furthermore, in step (3), the mushroom sticks are placed at intervals of 4 to 6 cm, and after the mushroom sticks are placed, the Armillaria mellea is placed at the fish-scale opening of the mushroom stick.

[0027] Beneficial effects:

[0028] 1. This invention improves the water retention capacity of coconut fiber by treating coconut soil, which can reduce the uneven water content in the substrate caused by water seepage downwards during the cultivation of Gastrodia elata, thus affecting its growth. In addition, the treated coconut soil has a loose structure, which can better decompose and release nutrients. Together with other raw materials in the substrate, it can ensure a more comprehensive supply of nutrients to the substrate, thus better promoting the germination and growth of Gastrodia elata.

[0029] 2. This invention uses coconut coir as the main raw material, mixed with corn steep liquor, wheat bran and other raw materials to prepare a Gastrodia elata breeding substrate. The breeding substrate is lightweight and has good heat retention, which can better meet the growth conditions of Gastrodia elata, promote seed germination, increase yield, and reduce labor costs in the breeding process, thus showing good application prospects. Attached Figure Description

[0030] Figure 1 : This is a diagram of the Gastrodia elata seed obtained from the breeding of experimental group 1 in Experiment 2 of this invention;

[0031] Figure 2 : This is a diagram of the Gastrodia elata seed obtained from the breeding of experimental group 1 in Experiment 2 of this invention. Detailed Implementation

[0032] The present invention will now be described in detail with reference to specific embodiments and accompanying drawings:

[0033] Example 1: Preparation of breeding substrate

[0034] A: Take 0.5 kg of tert-butylethanolamine, add it to 50 kg of water and stir to dissolve. Then add 40 kg of coconut fiber, heat to 70℃ and stir to react for 5 h. After the reaction is completed, sonicate for 40 min. Then add 1.5 kg of diethylene glycol dimethyl ether, stir to mix evenly, let stand for 2 days, filter to remove the filtrate to obtain the treated coconut fiber, and air dry the surface moisture under natural conditions for later use.

[0035] B: Take 0.5 kg of ammonium dihydrogen phosphate and 0.3 kg of magnesium sulfate, put them into 4 kg of water and stir to dissolve. Add the solution to 40 kg of dried treated coconut soil, then add 3 kg of corn syrup and 1.5 kg of wheat bran and stir to mix evenly. Adjust the moisture content to 55% and adjust the pH to 5.8 to obtain the breeding substrate.

[0036] Example 2: Preparation of breeding substrate

[0037] A: Take 0.4 kg of tert-butylethanolamine, add it to 40 kg of water and stir to dissolve. Then add 30 kg of coconut fiber, heat to 60℃ and stir to react for 6 hours. After the reaction is completed, sonicate for 30 minutes. Then add 1 kg of diethylene glycol dimethyl ether, stir to mix evenly, let stand for 1 day, filter to remove the filtrate to obtain the treated coconut fiber, and air dry the surface moisture under natural conditions for later use.

[0038] B: Take 0.4 kg of ammonium dihydrogen phosphate and 0.2 kg of magnesium sulfate, put them into 3 kg of water and stir to dissolve. Add the solution to 30 kg of dried treated coconut soil, then add 2 kg of corn syrup and 1 kg of wheat bran and stir to mix evenly. Adjust the moisture content to 50% and adjust the pH to 5.5 to obtain the breeding substrate.

[0039] Example 3: Preparation of breeding substrate

[0040] A: Take 0.6 kg of tert-butylethanolamine, add it to 60 kg of water and stir to dissolve it. Then add 50 kg of coconut fiber, heat it to 80℃ and stir to react for 5 h. After the reaction is completed, sonicate it for 60 min. Then add 2 kg of diethylene glycol dimethyl ether, stir and mix it evenly. After standing for 2 days, filter to remove the filtrate to obtain the treated coconut fiber. Let it air dry the surface moisture under natural conditions for later use.

[0041] B: Take 0.6 kg of ammonium dihydrogen phosphate and 0.4 kg of magnesium sulfate, put them into 5 kg of water and stir to dissolve. Add the solution to 50 kg of dried treated coconut soil, then add 4 kg of corn syrup and 2 kg of wheat bran and stir to mix evenly. Adjust the moisture content to 60% and adjust the pH to 6 to obtain the breeding substrate.

[0042] Comparative Example 1: Preparation of Breeding Substrate

[0043] In contrast to Example 1, the only difference is that in Comparative Example 1, tert-butylethanolamine was not added in step A of the breeding substrate preparation process; instead, water was directly added and heated for the reaction, as detailed below:

[0044] A: Add 40kg of coconut fiber to 50kg of water, heat to 70℃ and stir for 5 hours. After the reaction is complete, sonicate for 40 minutes. Then add 1.5kg of diethylene glycol dimethyl ether, stir and mix evenly. After standing for 2 days, filter to remove the filtrate to obtain the treated coconut fiber. Let it air dry under natural conditions for later use.

[0045] B: Same as Example 1.

[0046] Comparative Example 2: Preparation of Breeding Substrate

[0047] Compared with Example 1, the only difference is that diethylene glycol dimethyl ether was not added in step A of the breeding substrate preparation in Comparative Example 2, as detailed below:

[0048] A: Take 0.5 kg of tert-butylethanolamine, add it to 50 kg of water and stir to dissolve. Then add 40 kg of coconut fiber, heat to 70℃ and stir to react for 5 h. After the reaction is complete, sonicate for 40 min, let stand for 2 days, filter to remove the filtrate to obtain the treated coconut fiber, and air dry the surface moisture under natural conditions for later use.

[0049] B: Same as Example 1.

[0050] Comparative Example 3: Preparation of Breeding Substrate

[0051] Compared with Example 1, the only difference is that ultrasonic treatment was not performed in step A of the breeding substrate preparation in Comparative Example 3, as detailed below:

[0052] A: Take 0.5 kg of tert-butylethanolamine, add it to 50 kg of water and stir to dissolve. Then add 40 kg of coconut fiber, heat to 70℃ and stir to react for 5 hours. After the reaction is complete, add 1.5 kg of diethylene glycol dimethyl ether, stir to mix evenly, let stand for 2 days, filter to remove the filtrate to obtain the treated coconut fiber, and air dry the surface moisture under natural conditions for later use.

[0053] B: Same as Example 1.

[0054] Comparative Example 4: Preparation of Breeding Substrate

[0055] Compared with Example 1, the only difference is that in Comparative Example 4, the reaction in step A of the breeding substrate preparation was carried out at room temperature (25°C), as detailed below:

[0056] A: Take 0.5 kg of tert-butylethanolamine, add it to 50 kg of water and stir to dissolve. Then add 40 kg of coconut fiber and stir to react for 5 h at room temperature of 25 °C. After the reaction is completed, sonicate for 40 min. Then add 1.5 kg of diethylene glycol dimethyl ether, stir to mix evenly, let stand for 2 days, filter to remove the filtrate to obtain the treated coconut fiber, and air dry the surface moisture under natural conditions for later use.

[0057] B: Same as Example 1.

[0058] Comparative Example 5: Preparation of Breeding Substrate

[0059] Compared with Example 1, the only difference is that step A was omitted in the preparation of the breeding substrate in Comparative Example 5. Instead, coconut fiber was directly added to step B to prepare the breeding substrate, as detailed below:

[0060] Take 0.5 kg of ammonium dihydrogen phosphate and 0.3 kg of magnesium sulfate, put them into 4 kg of water and stir to dissolve. Add the solution to 40 kg of coconut soil, then add 3 kg of corn steep liquor and 1.5 kg of wheat bran and stir to mix evenly. Adjust the moisture content to 55% and adjust the pH to 5.8 to obtain the breeding substrate.

[0061] Comparative Example 6 (Blank Control): Preparation of Breeding Substrate

[0062] Compared with Example 1, the only difference is that in Comparative Example 6, coconut coir was not used in the preparation of the breeding substrate. Instead, conventional sandy soil and sawdust were mixed to prepare the breeding substrate, as detailed below:

[0063] Sandy soil and sawdust were mixed at a mass ratio of 5:1 to obtain a sandy soil-sawdust mixture. 0.5 kg of ammonium dihydrogen phosphate and 0.3 kg of magnesium sulfate were added to 4 kg of water and stirred to dissolve. The solution was then added to 40 kg of the sandy soil-sawdust mixture. 3 kg of corn steep liquor and 1.5 kg of wheat bran were added and stirred until well mixed. The moisture content was adjusted to 55%, and the pH was adjusted to 5.8 to obtain the breeding substrate.

[0064] Example 4: Bagged Gastrodia elata breeding method

[0065] (1) Preparation of breeding substrate: The breeding substrate was prepared using the method in Example 1;

[0066] (2) Seed dressing: Select germinating bacteria with good growth and high germination rate, decompose the germinating bacteria into particles about 1 cm in size, gently shake out the seeds from the capsule of Gastrodia elata and sprinkle them on the decomposed germinating bacteria, stirring while sprinkling to mix the seeds and germinating bacteria evenly. Mix the seeds according to the amount of 12 capsules of Gastrodia elata per kilogram of germinating bacteria. After mixing evenly, place at room temperature for 3 days to obtain the germinating bacteria-seed mixture, which can then be used for Gastrodia elata breeding.

[0067] (3) Breeding: Take a branch of Quercus acutissima with a diameter of about 6cm, cut it to a length of 15cm, and make 3 rows of fish-scale cuts reaching the xylem to obtain the mushroom log. Soak the mushroom log in clean water for 3 days in advance. Use a plastic bag as the breeding bag. First, lay a breeding substrate of about 5cm in the breeding bag, and then add 0.5kg / m 2 Sprinkle a layer of germination fungus-seed mixture evenly, then place the mushroom sticks at intervals of about 5cm. After placing the mushroom sticks, place the Armillaria mellea at the fish-scale opening of the mushroom sticks. Repeat the above operation twice: spreading the breeding substrate, sprinkling the germination fungus-seed mixture, and placing the mushroom sticks and Armillaria mellea. Finally, spread a layer of breeding substrate about 8cm thick on top, tie the breeding bag, and place it in a shaded area under the forest for breeding.

[0068] Experiment 1: Water-holding capacity test of breeding substrate

[0069] The water retention of the breeding substrates prepared in Example 1 and Comparative Examples 1-6 was determined using the following method: Seven flowerpots were filled with soil to half their capacity, and then the breeding substrates prepared in Example 1 and Comparative Examples 1-6 with a water content of 55% were filled on top, with a substrate thickness of 8 cm. The flowerpots were then sealed with plastic film and placed in a cool place for 20 and 40 days. The water content of the breeding substrate in each group was then measured. The experiment was repeated three times, and the data are shown in Table 1.

[0070] Table 1

[0071]

[0072]

[0073] Based on the data analysis in Table 1, we can conclude that:

[0074] (1) The breeding substrate prepared in Example 1 has a good water retention effect. After 20 days and 40 days, the substrate can still maintain a high water retention capacity, indicating that the breeding substrate prepared in this invention can lock in water well when used for Gastrodia elata cultivation and breeding, inhibit water seepage, and prevent excessive water seepage from causing the upper substrate to have too low water content and the lower substrate to have too high water content, thus affecting the growth of Gastrodia elata.

[0075] (2) In Comparative Example 1, tert-butylethanolamine was not used to treat the coconut fiber during the preparation of the breeding substrate. As a result, the abundant hydroxyl functional groups in the coconut fiber were not exposed, which greatly reduced the water-holding capacity of the substrate and caused a large amount of water loss. In Comparative Example 2, diethylene glycol dimethyl ether was not added during the preparation of the breeding substrate. As a result, the coconut fiber had poor expansion performance, and water molecules could occupy little space and were easily lost. In Comparative Example 3, ultrasonic treatment was not performed during the preparation of the breeding substrate. In Comparative Example 4, the breeding substrate was prepared under normal temperature conditions. Compared with Example 1, the water-holding capacity of the breeding substrates prepared in Comparative Examples 3 and 4 was greatly reduced. This is because under ultrasonic treatment and heating reaction conditions in Example 1, tert-butylethanolamine and other substances entered the coconut fiber more efficiently, thereby increasing the treatment effect.

[0076] Experiment 2: Bagged Gastrodia elata breeding experiment

[0077] In mid-May 2022, a bag-cultivation experiment of Gastrodia elata was conducted in Yingchuan Village, Pudi Township, Baili Rhododendron Management Area, Bijie City. The breeding substrates prepared in Example 1 and Comparative Examples 1-6 were used for Gastrodia elata breeding experiments. The experiment was divided into 7 groups: experimental group 1 and control groups 1-6. Experimental group 1 used the breeding substrate of Example 1 and the breeding method of Example 4; control groups 1-6 used the breeding substrates of Comparative Examples 1-6 and the breeding method of Example 4, respectively. Each group had 8 breeding bags, and each bag contained 6 Gastrodia elata capsule seeds. The average yield of Gastrodia elata seeds obtained from each group was recorded, and the data are shown in Table 2.

[0078] Table 2

[0079]

[0080] According to the data in Table 2:

[0081] (1) The yield of Gastrodia elata seeds in experimental group 1 was higher than that in control groups 1-6, and the obtained Gastrodia elata seeds showed good growth. Figure 1 , Figure 2 This indicates that by using coconut coir as a raw material to prepare a breeding substrate for the cultivation and breeding of Gastrodia elata according to the method of the present invention, the yield and quality of the Gastrodia elata seeds obtained through breeding can be significantly improved.

[0082] (2) In control groups 1 to 4, the water-holding capacity of the breeding substrate was reduced to varying degrees, and the unsuitable water content of the substrate affected the growth of Gastrodia elata, resulting in a reduction in the yield of Gastrodia elata seeds in control groups 1 to 4 to varying degrees.

[0083] (3) In control group 5, the coconut soil was not treated during the preparation of the breeding substrate. The coconut soil substrate has poor water retention and stable structure. The decomposable nutrients of germinating bacteria and Armillaria mellea are relatively reduced. Therefore, the yield of Gastrodia elata seeds in control group 5 is significantly reduced. In control group 6, conventional sand and sawdust are used as substrates. The substrate has poor water retention and causes compression to the growth of Gastrodia elata, resulting in a significant reduction in the yield of Gastrodia elata seeds.

[0084] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications and substitutions should be covered within the scope of the claims of the present invention. Technical aspects, shapes, and structures not described in detail in this invention are all well-known technologies.

Claims

1. A method for breeding Gastrodia elata in bags, characterized in that, The method is as follows: Step (1) Preparation of breeding substrate: Breeding substrate is prepared using coconut coir as raw material; Step (2) Seed mixing: Mix the germination fungus and Gastrodia elata seeds evenly to obtain a germination fungus-seed mixture; Step (3) Breeding: Use a plastic bag as a breeding bag. First, spread the breeding substrate evenly in the breeding bag, then evenly sprinkle a layer of germination fungus-seed mixture, then place the fungus sticks and Armillaria mellea, and repeat the operation of spreading the breeding substrate, sprinkling the germination fungus-seed mixture and placing the fungus sticks and Armillaria mellea twice. Finally, after spreading a layer of breeding substrate, tie the breeding bag and place it in a shaded area under the forest for breeding. The breeding substrate includes the following raw materials: Coconut clay, tert-butylethanolamine, diethylene glycol dimethyl ether, corn steep liquor, wheat bran, ammonium dihydrogen phosphate, magnesium sulfate; The method for preparing the breeding substrate is as follows: Step A: Dissolve tert-butylethanolamine in water and add it to coconut fiber. Heat the mixture to 60-80℃ and stir for 4-6 hours. After the reaction is complete, sonicate for 30-60 minutes. Then add diethylene glycol dimethyl ether and stir to mix evenly. After standing for 1-2 days, filter to remove the filtrate to obtain the treated coconut fiber. Let it air dry under natural conditions for later use. Step B: Dissolve ammonium dihydrogen phosphate and magnesium sulfate in water and add them to the dried treated coconut soil. Then add corn syrup and wheat bran and stir to mix evenly. Adjust the moisture content and pH to obtain the breeding substrate. In step A, the mass ratio of coconut fiber to tert-butylethanolamine and diethylene glycol dimethyl ether is (30-50):(0.4-0.6):(1-2). In step B, the mass ratio of ammonium dihydrogen phosphate, magnesium sulfate, treated coconut coir, corn steep liquor, and wheat bran is (0.4–0.6): (0.2–0.4): (30–50): (2–4): (1–2). In step B, the pH is adjusted to 5.5-6, and the water content is adjusted to 50-60%.

2. The method for bagged breeding of Gastrodia elata according to claim 1, characterized in that, The seed mixing operation in step (2) is as follows: First, decompose the germination bacteria into particles of 1-2 cm in size. Gently shake out the seeds from the Gastrodia elata capsules and sprinkle them onto the decomposed germination bacteria while sprinkling. Mix the seeds and germination bacteria evenly. Mix 10-16 Gastrodia elata capsules per kilogram of germination bacteria. After mixing evenly, let it stand at room temperature for 1-5 days to obtain the germination bacteria-seed mixture.

3. The method for bagged breeding of Gastrodia elata according to claim 2, characterized in that, The substrate used for the mushroom substrate is any one of the following: Oak, Quercus acutissima, Quercus variabilis, and Quercus cuspidata.

4. The method for bagged breeding of Gastrodia elata according to claim 3, characterized in that, The mushroom logs are 5-10 cm in diameter and 15-25 cm in length, and are cut with 2-3 rows of fish-scale-like cuts that reach the xylem.

5. The method for bagged breeding of Gastrodia elata according to claim 4, characterized in that, In step (3), the mushroom sticks are placed at intervals of 4-6cm. After the mushroom sticks are placed, the Armillaria mellea is placed at the fish-scale opening of the mushroom stick.

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