A kind of dwarf double-spined borer attractant composition and application

Abstract

The present invention discloses a dwarf two-spined borer attractant composition and application. The active substance in the dwarf two-spined borer attractant is composed of volatile substances from the surface of dwarf two-spined borer adults and active ingredients from volatile substances in Terminalia microphylla leaves, specifically 1 to 10 μg / μL of oleic acid, 1 to 10 μg / μL of petroselinic acid, 1 μg / μL of p-ethylacetophenone, 1 μg / μL of 3-ethylacetophenone, and 100 μg / μL of 1,3-diethylbenzene. The active substance is used as an effective ingredient and paraffin is used as a diluent to further obtain an attractant preparation. The preparation has a significant attraction effect on dwarf two-spined borer adults, is used in a small amount, is easy to use, has strong targeting, can be simultaneously applied to dwarf two-spined borer prevention and control and population dynamics monitoring, and is of great significance for the research and development of new green control technologies for dwarf two-spined borer.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and particularly relates to a dwarf double-spined borer attractant composition and application. Background Art

[0002] Sinoxylon spp. are stem-boring pests of urban forest plants. They have a varied diet and a wide host range, attacking a variety of broadleaf logs, newly sawn boards, square timber, and newly debarked logs. They also attack old, diseased, and weakened trees. Adults and larvae feed on the branches of host plants, as well as wooden products such as pallets, padding, and furniture, severely impacting plant growth, ornamental value, ecological benefits, and the lifespan of the finished boards. Because they hide in the xylem, they are difficult to detect and control, making this group of pests the most important pests of urban forest plants. The Sinoxylon pygmaeum (Coleoptera: Scolytidae) is a serious stem-boring pest of Terminalia mantaly, a widely cultivated urban forest plant in my country. It bores into the xylem and feeds, causing leaf drop in mild cases and, in severe cases, dieback of lateral branches or even complete hollowing of entire branches, impacting the plant's growth, development, landscape, and ecological impacts. Sinoxylon pygmaeum exhibits no obvious symptoms in the early stages of its infestation, only becoming apparent later, when the infestation is severe, after the optimal control period has passed. Under natural conditions, the insect's natural enemies have limited control capabilities; light trapping, commonly used in physical control, is ineffective; and chemical agents used in emergency control are ineffective and can easily pollute the environment and harm natural enemies. Therefore, there is an urgent need to explore green, healthy, and sustainable control technologies. Summary of the Invention

[0003] The purpose of the present invention is to provide a dwarf two-spined borer attractant composition and application, so as to provide technical support for the green prevention and control of the insect.

[0004] To achieve the above objectives, the present invention provides the following technical solutions:

[0005] A dwarf double-spined borer attractant composition, the active substances of which are composed of volatiles from the surface of adult beetles and plant volatiles, wherein the volatiles from the surface of adult beetles are oleic acid and petroselinic acid, and the plant volatiles are p-ethylacetophenone, 3-ethylacetophenone and 1,3-diethylbenzene.

[0006] The following scheme is further preferred:

[0007] A dwarf double-spined borer attractant composition, whose active substances consist of 1-10 μg / μL of oleic acid, 1-10 μg / μL of petroselinic acid, 1 μg / μL of p-ethylacetophenone, 1 μg / μL of 3-ethylacetophenone and 100 μg / μL of 1,3-diethylbenzene.

[0008] The following scheme is further preferred:

[0009] A dwarf double-spined borer attractant composition, whose active substances consist of 1 μg / μL of oleic acid, 1 μg / μL of petroselinic acid, 1 μg / μL of p-ethylacetophenone, 1 μg / μL of 3-ethylacetophenone and 100 μg / μL of 1,3-diethylbenzene.

[0010] The present invention also claims the use of the aforementioned Dwarf Dipsacillus attractant composition to prepare a Dwarf Dipsacillus attractant. The Dwarf Dipsacillus attractant is prepared by diluting adult volatiles and plant volatiles with liquid paraffin to a specific concentration, then adding a specific volume (5 μL) to the bait core. The prepared bait core is stored in a refrigerator at -20°C until ready for use.

[0011] The present invention also claims to protect the use of the dwarf two-spined borer attractant composition in the prevention and control of dwarf two-spined borer and population monitoring.

[0012] The present invention has the following beneficial effects:

[0013] The present invention proves through gas chromatography-electroantennary coupling technology (GC-EAD) and behavioral experiments that volatiles on the surface of adult beetles and plant volatiles can attract adult dwarf two-spined borers. The attractant composition of the present invention has a strong synergistic trapping effect on adult dwarf two-spined borers, is environmentally friendly, and harmless to humans. It can be accurately applied to the prediction, trapping and prevention of dwarf two-spined borers, and has good economic and ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The GC-EAD reaction of male and female adults of the present invention on the body surface extracts of the adults. A, GC-EAD reaction of the antennae of male and female adults of the present invention on the body surface extracts of the female adult; B, GC-EAD reaction of the antennae of male and female adults of the present invention on the body surface extracts of the male adult.

[0015] Figure 2 This is the GC-EAD reaction of male and female adults of the dwarf borer to volatiles from Terminalia microphylla branches.

[0016] Figure 3Figure 2 shows the directional behavioral responses of male and female adults of the present invention to four different concentrations of oleic acid and petroselinic acid extracts from their body surfaces. A, Selection rate of female dwarf ...

[0017] Figure 4 The directional behavioral responses of male and female adults of the dwarf schizophora to the optimal ratio of two combinations of body surface extracts. A, Selection rate of male and female adults of the dwarf schizophora to a mixture of 1 μg / μL oleic acid and petroselinic acid plus 1 μg / μL; B, Selection rate of male and female adults of the dwarf schizophora to a mixture of 1 μg / μL oleic acid and petroselinic acid plus 10 μg / μL. Data in the figure are mean ± standard error. * indicates a significant difference between the treatment and the control at the P < 0.05 level; NS indicates no significant difference between the treatment and the control at the P < 0.05 level (independent sample t-test).

[0018] Figure 5 The directional behavioral responses of male and female adults of the present invention to four different concentrations of the Terminalia microphylla branch volatiles p-ethylacetophenone, 3-ethylacetophenone, and 1,3-diethylbenzene. A, Selection rate of female dwarf ...

[0019] Figure 6The directional behavioral responses of male and female adults of the dwarf double-spined borer to four different concentration mixtures of three active ingredients from Terminalia microphylla volatiles. A, The selection rate of male and female adults of the dwarf double-spined borer to a mixture of 1 μg / μL + 1 μg / μL of p-ethylacetophenone and 3-ethylacetophenone; B, The selection rate of male and female adults of the dwarf double-spined borer to a mixture of 1 μg / μL + 100 μg / μL of p-ethylacetophenone and 1,3-diethylbenzene; C, The selection rate of male and female adults of the dwarf double-spined borer to a mixture of 1 μg / μL + 100 μg / μL of 3-ethylacetophenone and 1,3-diethylbenzene. D, The selection rate of male and female adults of Diplodocus dwarfus to the mixture of p-ethylacetophenone, 3-ethylacetophenone and 1,3-diethylbenzene at the ratio of 1 μg / μL+1 μg / μL+100 μg / μL; The data in the figure are mean ± SD; * indicates that the difference between the treatment and the control is significant at the P < 0.05 level; NS indicates that the difference between the treatment and the control is not significant at the P < 0.05 level (independent sample t test).

[0020] Figure 7 This figure shows the directional behavioral responses of male and female adults of the dwarf borer to the optimal ratio of active ingredients from body surface extracts and Terminalia microphylla volatiles. Data in the figure are mean ± standard error; NR indicates individuals that did not respond to the treatment; N indicates the total sample size; ** and * indicate significant differences between the treatment and the control at P < 0.01 and P < 0.05, respectively; NS indicates no significant difference between the treatment and the control at P < 0.05 (independent sample t-test). DETAILED DESCRIPTION

[0021] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the accompanying drawings and specific embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1

[0023] Collection, analysis and determination of active substances from the body surface extracts of male and female adults of Diplocera dwarfi

[0024] Collection of active substances from the body extracts of male and female adults of the dwarf beetle: Surface volatiles were collected using a solution extraction method. The entire beetle was soaked in an organic solvent, with 5 μL of chromatography-grade n-hexane added per beetle. The extraction was carried out at room temperature for 30 minutes. The supernatant was aspirated, filtered, and transferred to a clean chromatography vial. The supernatant was concentrated to the required volume using nitrogen gas before instrumental analysis. In this experiment, chromatography-grade n-hexane was used to extract the surface volatiles.

[0025] GC-MS conditions: The GC column was an HP-5MS capillary column (30 m × 250 μm × 0.25 μm, Agilent Technologies), with a 1 μL injection volume and splitless injection. The inlet temperature was 250°C, and the temperature program was as follows: initial temperature 60°C, hold for 2 min, then increase to 250°C at a rate of 10°C / min and hold for 5 min. The ionization mode was EI, with an ionization energy of 70 eV and a mass scan range of 30–500 amu. The components were qualitatively analyzed by comparing their mass spectra with those of standard compounds in the NIST 17.0 standard library.

[0026] GC-EAD reactions to surface extracts of male and female adult dwarf beetles: First, use a sharp scalpel to sever the antennae of active male and female dwarf beetles from their bases, removing a small portion of the distal end of the telophase. Then, moisten the antennae with saline. Connect the head and antennae to the reference and recording electrodes, respectively. The column temperature ramp is the same as for GC-MS. Crude surface extracts of male and female adult beetles are tested, using live adult beetles. Three replicates are performed. The peak shapes and retention times of the compounds on the GC-EAD chromatograms are compared. Combined with the GC-MS results, the substances that induce electrophysiological reactions in the antennae of male and female dwarf beetles are identified.

[0027] like Figure 1 As shown, the extracts from the body surface of female dwarf beetles were analyzed by GC-EAD, and the substances that can induce electroantennary responses in male and female beetles are: octadecane, docosane, cis-9-tricosene, n-pentacosane, Octadecyl2-chloropropanoate, 2-Heptadecanol, n-nonacosane, oleic acid, 9-methylnonadecane, n-heneicosane, heptadecane, and petroselinic acid. Among them, Figure 1 In A, peak 1 is octadecane, peak 2 is 9-methylnonadecane, peak 3 is n-nonacosane, peak 4 is octadecyl 2-chloropropionate, peak 5 is oleic acid, peak 6 is 11-decylhexanedecane, peak 7 is 9-octylheptadecane, and peak 8 is cis-6-octadecenoic acid. Figure 1 In B, peak 1 is octadecane, peak 2 is docosane, peak 3 is entelophene, peak 4 is pentacosane, peak 5 is oleic acid, peak 6 is 2-heptadecanol, and peak 7 is octadecenoic acid.

[0028] Example 2

[0029] Collection, Analysis and Determination of Volatiles from Terminalia microphylla Branches

[0030] Collection of Terminalia microphylla branch volatiles: Branch volatiles were collected using dynamic headspace adsorption. Healthy Terminalia microphylla branches that had not been sprayed with chemical agents were selected. A sampling bag was placed around 8 to 10 healthy branches, and the sampling device was connected sequentially with Teflon tubing. The inlet was connected to an activated carbon drying tower, and the outlet was connected to an adsorption tube (15 cm long, 0.3 cm inner diameter). The adsorption tube was filled with 100 mg of adsorbent and then connected to an atmospheric sampler. Before sampling, the seal was checked. The sampling bag was evacuated and then inflated. Sampling was then performed at a controlled airflow of 200 ml / min. Each collection lasted 8 hours and was repeated three times. After collection, the adsorption tube was immediately sealed with aluminum foil and parafilm, placed in an ice box, and brought back to the laboratory. After elution with 1 ml of chromatographic-grade n-hexane, the sample was stored in a refrigerator (-18°C) until testing.

[0031] The analysis and determination were carried out according to the GC-MS detection conditions and GC-EAD test method in Example 1.

[0032] like Figure 2 As shown in the figure, the volatiles of Terminalia microphylla branches were analyzed by GC-EAD, and the substances that can induce the electroantennal response of male and female dwarf beetles are: cyclohexane, heptane, octane, decane, 1,3-diethylbenzene, m-isopropyl toluene, p-isopropylbenzyl alcohol, carvacrol, 3-ethylacetophenone and p-ethylacetophenone. Among them, Figure 2 Among them, peak 1 and peak 2 are both 1,3-diethylbenzene, peak 3 is p-cymene 1-methyl-3-(1-methylethyl)-benzene, peak 4 is 3-ethylacetophenone, and peak 5 is p-ethylacetophenone.

[0033] Example 3

[0034] Directional behavioral responses of male and female adults of the dwarf borer to a single active substance extracted from their body surface

[0035] The single active ingredients of the body surface extract, oleic acid and petroselinic acid, were selected, and paraffin was used as the solvent. The two active substances of the body surface extract, oleic acid and petroselinic acid, were diluted into four concentration gradients of 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL, and paraffin was used as the control.

[0036] During the test, 5 μL of the sample and liquid paraffin were dropped onto 1 cm × 2 cm qualitative filter papers, and placed in the odor source chambers of the two arms of the "Y" olfactometer, with liquid paraffin as the control. During the test, 5 μL of the sample and liquid paraffin were dropped onto 1 cm × 2 cm qualitative filter papers, and placed in the odor source chambers of the two arms of the "Y" olfactometer, with liquid paraffin as the control. Ventilate for 30 seconds to allow the odor to fully flow through the olfactometer. The gas flow rate was controlled at 300 mL min -1. Only one virgin adult female / male of the dwarf double-spined borer was released each time. Each adult was observed for 5 minutes. Those that entered more than half of the side arm and stayed there for 30 seconds were counted as choosing, otherwise it was recorded as no response and the adult was replaced and retested. Each adult was used only once. Ten adults were tested in each group, and 5 groups were repeated. After every 5 adults were tested, the position of the tube walls on both sides of the olfactometer was swapped to eliminate environmental influences. After each group was tested, the olfactometer and connecting tube were cleaned with anhydrous ethanol. The number of adults that had behavioral responses to the treatment and control was recorded.

[0037] Statistical analysis: The independent sample T-test was used to analyze the single behavioral responses of the dwarf beetle to the body surface extracts.

[0038] like Figure 3 -A shows that the selection rates of female dwarf two-spined borer to oleic acid concentrations of 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL were 47.9±11.0% (t6=-0.277, P=0.761), 62.4±7.7% (t6=1.567, P=0.168), 57.1±8.5% (t6=0.758, P=0.477) and 47.5±4.8% (t6=-0.397, P=0.705), respectively, and the selection rates for the control were 52.1±11.0%, 37.6±7.7%, 42.9±8.5% and 52.5±4.8%, respectively. There was no significant difference between the treatments and the control.

[0039] like Figure 3 -B shows that the selection rate of male insects for oleic acid concentration of 1μg / μL was 67.7±2.6%, and the selection rate for the control was 32.3±2.6%, which were significantly higher than those of the control (t6=3.464, P<0.05); while the selection rates for 0.1μg / μL, 10μg / μL and 100μg / μL were 53.8±7.5% (t6=0.397, P=0.705), 70.1±11.3% (t6=1.414, P=0.207) and 55.5±16.4% (t6=0.190, P=0.855), respectively, and the selection rates for the control were 46.2±7.5%, 29.9±11.3% and 44.5±16.4%, respectively. There was no significant difference between the treatments and the control.

[0040] like Figure 3-C shows that the selection rates of female dwarf two-spined borers to petroselinic acid concentrations of 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL were 60.0±8.2% (t6=1.732, P=0.134), 56.7±7.9% (t6=0.632, P=0.550), 57.5±16.5% (t6=0.471, P=0.654) and 57.1±13.4% (t6=0.000, P=1.000), respectively, and the selection rates to the control were 40.0±8.2%, 43.3±7.9%, 42.5±16.5% and 42.9±13.4%, respectively. There was no significant difference between the treatments and the control.

[0041] like Figure 3 -D shows that the selection rates of male insects to 1μg / μL and 10μg / μL petroselinic acid concentrations were 76.7±9.3% (t6=2.554, P<0.05) and 69.3±10.3% (t6=2.530, P<0.05), respectively, while the selection rates to the control were 23.3±9.3% and 30.7±10.3%, respectively, which were significantly higher than the control; while the selection rates to 0.1μg / μL and 100μg / μL were 45.9±5.3% (t6=-0.562, P=0.595) and 55.0±9.6% (t6=0.739, P=0.488), respectively, while the selection rates to the control were 44.1±5.3 and 45.0±9.6%, respectively. There was no significant difference between the treatments and the control.

[0042] Example 4

[0043] Orientational behavioral responses of male and female adults of Diplocera pygmystris to a mixture of body surface extracts

[0044] like Figure 4 As shown, the mixture of the surface extract of the adult dwarf beetle comprises:

[0045] Ratio 1: 1 μg / μL oleic acid and 1 μg / μL petroselinic acid.

[0046] Ratio 2: 1 μg / μL oleic acid and 10 μg / μL petroselinic acid.

[0047] The directional behavioral responses of male and female adults of the dwarf borer to the optimal concentration mixture of the two body surface extracts were studied using a "Y" type olfactometer.

[0048] During the test, 5 μL of the mixture and paraffin were each dropped onto 1 cm x 2 cm qualitative filter paper. The paper was then placed in the odor source chambers of the two arms of a "Y"-shaped olfactometer, with paraffin serving as a control. The behavioral responses of male and female adults of the dwarf beetle to the mixture of body surface extracts were measured using the test and analysis methods described in Example 3.

[0049] like Figure 4 As shown in Figure 1-A, the female beetles of the dwarf beetle had a selection rate of 72.9±5.5% (t8=2.959, P<0.05) for the surface extract mixture 1, compared to 27.1±5.5% for the control, which was significantly higher than the control. The male beetles had a selection rate of 71.9±6.5% (t8=2.959, P<0.05) for the surface extract mixture 1, compared to 28.1±6.5% for the control, which was significantly higher than the control.

[0050] like Figure 4 As shown in Figure 2B, females of the dwarf beetle had a selection rate of 47.0±6.8% (t8=2.959, P=0.343) for the surface extract mixture 2, compared to 53.0±6.8% for the control, showing no significant difference from the control. Males had a selection rate of 58.6±10.2% (t8=1.086, P=0.320) for the surface extract mixture 2, compared to 41.4±10.2% for the control, showing no significant difference from the control.

[0051] Example 5

[0052] Behavioral responses of male and female adults of Diplocera pygmystriata to single active substances from plant volatiles

[0053] Single active ingredients of plant volatiles, p-ethylacetophenone, 3-ethylacetophenone and 1,3-diethylbenzene, were selected and diluted with paraffin as solvent to four concentration gradients of 0.1 μg / μL, 1 μg / μL, 10 μg / μL and 100 μg / μL, with paraffin as the control.

[0054] During the test, 5 μL of the test sample and paraffin were each dropped onto 1 cm x 2 cm qualitative filter papers. The papers were then placed in the odor source chambers of the two arms of a "Y"-shaped olfactometer, with paraffin serving as a control. The behavioral responses of male and female adults of the dwarf borer to a single active ingredient from Terminalia microphylla were measured using the test and analysis methods described in Example 3.

[0055] like Figure 5 -A shows that the selection rates of female dwarf two-spined borer to 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL of p-ethylacetophenone were 52.5±2.5% (t6=0.000, P=1.000), 48.1±7.8% (t6=0.000, P=1.000), 51.8±1.8% (t6=0.361, P=0.730) and 61.1±1.5% (t6=-1.439, P=0.200), respectively, and the selection rates to the control were 47.5±2.5%, 51.9±7.8%, 48.2±1.8% and 38.9±1.5%, respectively. There was no significant difference between the treatments and the control.

[0056] like Figure 5 -B shows that the selection rate of male insects for 1μg / μL of p-ethylacetophenone was 58.5±13.3%, and the selection rate for the control was 41.5±13.3%, which were significantly higher than those of the control (t6=3.396, P<0.05); while the selection rates for 0.1μg / μL, 10μg / μL and 100μg / μL were 53.1±8.4% (t6=0.471, P=0.654), 42.3±13.7% (t6=-1.177, P=0.284) and 38.9±6.5% (t6=2.236, P=0.067), respectively, and the selection rates for the control were 46.9±8.4%, 57.7±13.7% and 61.1±6.5%, respectively. There were no significant differences between the treatments and the control.

[0057] like Figure 5 -C shows that the selection rate of female dwarf beetles to 3-ethylacetophenone at a concentration of 1 μg / μL was 70.1±8.7%, while the selection rate to the control was 29.9±8.7%, which were significantly higher than those of the control (t6=2.923, P<0.05); while the selection rates to 0.1 μg / μL, 10 μg / μL and 100 μg / μL were 42.4±13.5% (t6=-0.905, P=0.401), 44.4±9.1% (t6=-0.880, P=0.413) and 55.7±14.5% (t6=0.139, P=0.894), respectively, while the selection rates to the control were 57.6±13.5%, 55.6±9.1% and 44.2±14.5%, respectively. There were no significant differences between the treatments and the control.

[0058] like Figure 5 -D shows that the selection rates of male insects for 3-ethylacetophenone at concentrations of 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL were 52.5±5.7% (t6=0.655, P=0.537), 71.1±12.5% ​​(t6=2.144, P=0.076), 64.6±10.0% (t6=1.886, P=0.108) and 55.2±15.3% (t6=-0.545, P=0.606), respectively, and the selection rates for the control were 47.5±5.7%, 28.9±12.5%, 35.4±10.0% and 44.8±15.3%, respectively. There were no significant differences between the treatments and the control.

[0059] like Figure 5-E shows that the selection rate of female dwarf beetles to 1,3-diethylbenzene at a concentration of 100 μg / μL was 70.4±4.9%, while the selection rate to the control was 29.6±4.9%, which were significantly higher than those of the control (t6=3.667, P<0.05); while the selection rates to 0.1 μg / μL, 1 μg / μL and 10 μg / μL were 66.5±8.1% (t6=2.377, P=0.055), 65.0±11.9% (t6=2.211, P=0.736) and 41.5±4.6% (t6=-1.464, P=0.194), respectively, while the selection rates to the control were 33.5±8.1%, 35.0±11.9% and 58.5±4.6%, respectively. There were no significant differences between the treatments and the control.

[0060] like Figure 5 -F, the selection rates of male insects for 1,3-diethylbenzene concentrations of 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL were 23.2±13.5% (t6=-1.711, P=0.537), 56.3±8.5% (t6=1.028, P=0.344), 28.8±11.3% (t6=-1.904, P=0.106) and 31.9±5.7% (t6=-2.000, P=0.092), respectively, and the selection rates for the control were 76.8±13.5%, 43.7±8.5%, 71.2±11.3% and 68.1±5.7%, respectively. There were no significant differences between the treatments and the control.

[0061] Example 6

[0062] Orientational behavioral responses of male and female adults of the dwarf borer to a mixture of plant volatiles

[0063] A mixture of volatiles from Terminalia microphylla is prepared, comprising:

[0064] Ratio 3: 1 μg / μL of p-ethylacetophenone and 1 μg / μL of 3-ethylacetophenone.

[0065] Ratio 4: 1 μg / μL of p-ethylacetophenone and 100 μg / μL of 1,3-diethylbenzene.

[0066] Ratio 5: 1 μg / μL 3-ethylacetophenone and 100 μg / μL 1,3-diethylbenzene.

[0067] Ratio 6: 1 μg / μL of p-ethylacetophenone, 1 μg / μL of 3-ethylacetophenone, and 100 μg / μL of 1,3-diethylbenzene.

[0068] A "Y"-type olfactometer was used to study the directional behavioral responses of male and female adults of the dwarf borer to the optimal concentration mixture of these four plant volatiles.

[0069] During the test, 5 μL of the mixture and paraffin were each dropped onto 1 cm x 2 cm qualitative filter paper. The paper was then placed in the odor source chambers of the two arms of a "Y"-shaped olfactometer, with paraffin serving as a control. The behavioral responses of male and female adults of the dwarf pygmy borer to the optimal concentration of the mixture of plant volatiles were measured using the experimental and analytical methods described in Example 3.

[0070] like Figure 6 As shown in Figure 1-A, females of the dwarf dwarf borer had a selection rate of 51.9±1.9% (U=3.000, P=0.456) for the optimal plant volatile mixture 3, compared to 48.1±1.9% for the control, with no significant difference between the treatments. Males had a selection rate of 53.7±1.9% (U=1.500, P=0.114) for the optimal plant volatile mixture 3, compared to 46.3±1.9% for the control, with no significant difference between the treatments and the control.

[0071] like Figure 6 As shown in Figure 3B, females of the dwarf borer exhibited a 44.9 ± 16.5% preference for the optimal plant volatile mixture (t6 = -0.343, P = 0.749), compared to 55.1 ± 16.5% for the control, with no significant difference between the treatments. Males exhibited a 41.3 ± 12.5% ​​preference for the optimal plant volatile mixture (U = 3.000, P = 0.487), compared to 58.7 ± 12.5% ​​for the control, with no significant difference between the treatments and the control.

[0072] like Figure 6 As shown in Figure 3-C, female D. pygmyspinosa had a selection rate of 50.9±15.4% for the optimal plant volatile mixture of Ratio 5 (t6=0.000, P=1.000), compared to 49.1±15.4% for the control, with no significant difference between the treatments and the control. Males had a selection rate of 34.2±4.6% for the optimal plant volatile mixture of Ratio 5 (t6=0.000, P=1.000), compared to 65.8±4.6% for the control, significantly higher than the control (U=0.000, P<0.05).

[0073] like Figure 6As shown in Figure 3-D, female D. pygmyspinosa selected 70.6±2.4% of the plant volatile mixture at its optimal concentration, compared to 29.4±2.4% of the control (U=0.000, P<0.05). Males selected 41.2±15.5% of the plant volatile mixture at its optimal concentration, compared to 58.8±15.5% of the control (t8=-0.857, P=0.440), with no significant difference between the treatments and the control.

[0074] Example 7

[0075] Orientational behavioral responses of male and female adults of the dwarf borer to a mixture of body surface extracts and plant volatiles

[0076] Preparation of topical extracts and plant volatile mixtures, including:

[0077] Ratio 7: 1 μg / μL oleic acid, 1 μg / μL petroselinic acid, 1 μg / μL p-ethylacetophenone, 1 μg / μL 3-ethylacetophenone and 100 μg / μL 1,3-diethylbenzene.

[0078] The directional behavioral responses of male and female adults of Diplocera pygmystris to the optimal concentration mixture of body surface extracts and plant volatiles (7) were studied using a "Y" type olfactometer.

[0079] During the test, 5 μL of the mixture and paraffin were dropped onto 1 cm x 2 cm qualitative filter papers, respectively. The papers were then placed in the odor source chambers of the two arms of a "Y"-shaped olfactometer, with paraffin serving as a control. The behavioral responses of male and female adults of the dwarf borer to the optimal concentration of the mixture of surface extracts and plant volatiles (7) were measured using the experimental and analytical methods described in Example 3.

[0080] like Figure 7 As shown, females of the dwarf beetle exhibited a selectivity of 51.9±3.7% (U=3.000, P=0.456) for the optimal mixture of body surface extracts and plant volatiles at concentrations of 7, compared to 48.1±3.7% for the control, with no significant difference between the treatments and the control. Males exhibited a selectivity of 71.9±5.9% for the optimal mixture of body surface extracts and plant volatiles at concentrations of 7, compared to 28.1±5.9% for the control, significantly higher than the control (U=0.000, P<0.043).

[0081] The above disclosure is only a specific embodiment of the present invention, but the present invention is not limited thereto. Any changes that can be conceived by those skilled in the art should fall within the scope of protection of the present invention.

Claims

1. A dwarf beetle attractant composition, characterized in that: The active substance consists of 1 μg / μL of oleic acid, 1 μg / μL of petroselinic acid, 1 μg / μL of p-ethylacetophenone, 1 μg / μL of 3-ethylacetophenone and 100 μg / μL of 1,3-diethylbenzene.

2. Use of the dwarf two-spined borer attractant composition according to any one of claim 1 to prepare a dwarf two-spined borer attractant.

3. Use of the attractant composition of dwarf two-spined borer according to any one of claims 1 in the prevention and control of dwarf two-spined borer and population monitoring.

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