A hydrogel for antibacterial treatment of skin surface and preparation method thereof

Abstract

The invention discloses a hydrogel for skin surface antibacterial and a preparation method thereof, belonging to the field of antibacterial gel. The present invention first modifies 6-amino-β-cyclodextrin to obtain acryloyl cyclodextrin (Ac-βCD); then adamantane is grafted onto hyaluronic acid to obtain adamantane-hyaluronic acid (Ad-HA); acrylyl cyclodextrin (Ac-βCD) is dispersed in water to obtain aqueous solution I; adamantane-hyaluronic acid (Ad-HA), an antibacterial agent, a moisturizer and a photoinitiator are dispersed in water to obtain aqueous solution II; aqueous solution I and aqueous solution II are mixed, left to stand, and ultraviolet irradiation is performed to obtain a hydrogel for skin surface antibacterial. The hydrogel for skin surface antibacterial in the present invention can be directly applied to the skin surface and kept moist for a long time, and its antibacterial rate for Gram-negative bacteria and Gram-positive bacteria reaches more than 99%, effectively inhibiting bacterial growth and protecting human health.

Description

Technical Field

[0001] The invention relates to a hydrogel for antibacterial treatment of skin surface and a preparation method thereof, belonging to the field of antibacterial gel dressings. Background Art

[0002] The skin is the largest organ in the human body, and it has the functions of protection, excretion, regulating body temperature, and sensing external stimuli. However, when the skin is damaged by external factors or the innate immune system is damaged, it becomes vulnerable to bacteria or viruses and causes infection. Prevention is the simplest way to reduce transmission, and proper cleaning and hygiene are the most effective strategies to resist the "pandemic" and control infection.

[0003] Antibacterial hand sanitizers, antibacterial hydrogels, etc. are common commercial antibacterial agents in life. These commercial antibacterial agents are almost products that directly add a single antibacterial agent. Long-term use of low-concentration antibacterial agents can easily lead to the formation of bacterial resistance. Traditional antibacterial agents have some defects, including poor skin compliance, poor permeability of active ingredients, and short antibacterial time. Moreover, frequent use of alcohol-containing hand sanitizers can deprive the skin of sebum and moisture, which can easily cause skin damage, including dermatitis, dry or cracked skin, as well as peeling, redness or itching, and reduce its ability to fight against another bacteria / virus (Ismail A, Raya NR, Orabi A, et al. Antibiotics, 2022, (11): 1606).

[0004] Chlorhexidine gluconate (CHG) is one of the most commonly used antibacterial agents, which is highly effective against Gram-positive and Gram-negative bacteria. CHG is widely used in local skin treatments, such as preoperative skin disinfection and hand washing. Ordinary antibacterial solutions containing CHG have rapid antibacterial activity. However, when these liquid antibacterial agents are applied to the skin, the active ingredients are easily removed from the skin, resulting in a very short effective antibacterial time.

[0005] Chlorhexidine gluconate (CHG) is also used to prepare antibacterial wound dressings or thermosensitive gels. For example, the literature (Lu Jinhua, Liu Qingcui, Su Hongrui. Preparation and prescription research of chlorhexidine gluconate thermosensitive gel [J]. Today's Pharmacy, 2016, 026 (002): 90-91, 94.) discloses the preparation of thermosensitive gel using poloxamer 407 as a matrix, but it is not suitable for application on the body surface; the literature (Apichaya Kapanya; Runglawan Somsunan; Robert Molloy; Supat Jiranusornkul; Worraanong Leewattanapasuk; Leena Jongpaiboonkit; Yen Kong. Synthesis of polymeric hydrogels incorporating chlorhexidine gluconate as antibacterial wound dressings [J]. Journal of Biomaterials Science, Polymer Edition, 2020, Vol. 31(7): 895-909) discloses an antibacterial hydrogel based on poly(sodium 2-acrylamide-2-methylpropane sulfonate) and gelatin combined with chlorhexidine gluconate (CHG) to prepare drugs, but it cannot adapt to irregularly shaped wounds.

[0006] Therefore, there is a great demand in the market for skin antibacterial agents that are safe, non-toxic, have long-term effectiveness, and have mechanical properties that are compatible with the skin. Summary of the invention

[0007] [Technical issues]

[0008] Conventional antibacterial agents used on the skin have poor skin compliance, poor penetration of active ingredients, short antibacterial time, and poor mechanical strength, flexibility and skin adaptability.

[0009] [Technical solution]

[0010] In order to solve the above problems, the present invention first modifies 6-amino-β-cyclodextrin to obtain acryloyl cyclodextrin (Ac-βCD); then adamantane is grafted onto hyaluronic acid to obtain adamantane-hyaluronic acid (Ad-HA); acrylyl cyclodextrin (Ac-βCD) is dispersed in water to obtain aqueous solution I; adamantane-hyaluronic acid (Ad-HA), an antibacterial agent, a moisturizer and a photoinitiator are dispersed in water to obtain aqueous solution II; aqueous solution I and aqueous solution II are mixed, allowed to stand, and ultraviolet irradiated to obtain a hydrogel for skin surface antibacterial. The hydrogel for skin surface antibacterial prepared by the present invention can be directly applied to the skin surface and keep it moist for a long time. Its antibacterial rate for Gram-negative bacteria and Gram-positive bacteria is more than 99%, which effectively inhibits bacterial growth and protects human health.

[0011] The first object of the present invention is to provide a method for preparing a hydrogel for antibacterial treatment of skin surface, comprising the following steps:

[0012] (1) dispersing acryloyl cyclodextrin (Ac-βCD) in water to obtain aqueous solution I;

[0013] (2) dispersing adamantane-hyaluronic acid (Ad-HA), an antibacterial agent, a moisturizing agent and a photoinitiator in water to obtain aqueous solution II;

[0014] (3) The aqueous solution I and the aqueous solution II are mixed, allowed to stand, and irradiated with ultraviolet light to obtain a hydrogel for antibacterial treatment of the skin surface.

[0015] In one embodiment of the present invention, the preparation method of acryloyl cyclodextrin (Ac-βCD) in step (1) is:

[0016] 6-amino-β-cyclodextrin was dissolved in water and modified by acryloyl chloride to obtain acryloyl cyclodextrin (Ac-βCD);

[0017] Specific;

[0018] 1 g of 6-amino-β-cyclodextrin was dissolved in an alkaline solution, the pH was adjusted to 10, acryloyl chloride was added in an ice bath to react, and after the reaction was completed, the solution was concentrated, precipitated with acetone, filtered, washed, and dried to obtain acryloyl cyclodextrin (Ac-βCD);

[0019] Among them, the mass ratio of 1g 6-amino-β-cyclodextrin and acryloyl chloride is 1g:96mg;

[0020] The alkaline solution is an aqueous solution of sodium bicarbonate;

[0021] The reaction time is 10h.

[0022] In one embodiment of the present invention, the concentration of the aqueous solution I in step (1) is 2-4% (w / v, g / mL).

[0023] In one embodiment of the present invention, the preparation method of adamantane-hyaluronic acid (Ad-HA) in step (2) is:

[0024] Hyaluronic acid is dissolved in water, and adamantane is grafted onto hyaluronic acid through a carbodiimide coupling reaction to obtain adamantane-hyaluronic acid (Ad-HA); the molecular weight of hyaluronic acid is 40kDa-4000kDa;

[0025] Specifically,

[0026] Dissolving hyaluronic acid (HA) in water to obtain a hyaluronic acid aqueous solution; wherein the amount ratio of hyaluronic acid (HA) to water is 1 g:90 mL;

[0027] Dissolve 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC), 1-hydroxybenzotriazole (HOBt) and 1-adamantanemethane in dimethyl sulfoxide (DMSO) to obtain a mixed solution; wherein the amount ratio of 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC), 1-hydroxybenzotriazole (HOBt), 1-adamantanemethane and dimethyl sulfoxide (DMSO) is 610 mg: 50 mg: 90 mg: 250 mL;

[0028] The mixed solution and the hyaluronic acid aqueous solution were mixed and reacted at 45° C. for 24 hours. After the reaction, acetone was used for precipitation, and the mixture was filtered and washed to obtain adamantane-hyaluronic acid (Ad-HA).

[0029] In one embodiment of the present invention, the concentration of adamantane-hyaluronic acid (Ad-HA) in the aqueous solution II of step (2) is 2-4% (w / v, g / mL); the concentration of the moisturizer is 4-6% (w / v, g / mL); the concentration of the photoinitiator is 0.4-0.6% (w / v, g / mL); the concentration of the antibacterial agent is 0.2-2% (w / v, g / mL); the antibacterial agent is a cationic antibacterial agent, including one or more of chlorhexidine gluconate (CHG), benzalkonium chloride, and benzethonium chloride.

[0030] In one embodiment of the present invention, in step (2), when the antibacterial agent is chlorhexidine glucose (CHG), it is added in the form of an aqueous solution, and the concentration of the chlorhexidine glucose (CHG) aqueous solution is 15-20% (w / v, g / mL).

[0031] In one embodiment of the present invention, the moisturizing agent in step (2) is one or more of polyols such as glycerol, propylene glycol, and butylene glycol.

[0032] In one embodiment of the present invention, the photoinitiator in step (2) is photoinitiator 2959.

[0033] In one embodiment of the present invention, the volume ratio of aqueous solution I to aqueous solution II in step (3) is 1:5-5:1.

[0034] In one embodiment of the present invention, the standing in step (3) is 20-30° C. (normal temperature) for 8-12 minutes.

[0035] In one embodiment of the present invention, the ultraviolet irradiation in step (3) is ultraviolet light with a wavelength of 365 nm and an irradiation time of 5-30 min.

[0036] The second object of the present invention is the hydrogel for skin surface antibacterial prepared by the method of the present invention.

[0037] The third object of the present invention is the use of the hydrogel for skin surface antibacterial according to the present invention in the field of wound dressings or cleansers.

[0038] The fourth object of the present invention is to provide a hand washing gel, which uses the hydrogel for antibacterial treatment of skin surface of the present invention.

[0039] The fifth object of the present invention is to provide a skin antibacterial agent, which uses the hydrogel for antibacterial on the skin surface described in the present invention.

[0040] [Beneficial Effects]

[0041] (1) The hydrogel prepared by the present invention for antibacterial treatment of skin surface is mild and comfortable, and has no toxic side effects.

[0042] (2) The hydrogel for skin surface antibacterial prepared by the present invention can be adhered to the skin to provide long-term release of antibacterial agents.

[0043] (3) The hydrogel for antibacterial treatment of the skin surface prepared by the present invention can moisturize the skin for a long time and keep it moist.

[0044] (4) The hydrogel for skin surface antibacterial prepared by the present invention has good mechanical properties and can adapt to the skin so as not to break when used in active areas.

[0045] (5) The hydrogel for skin surface antibacterial prepared by the present invention achieves encapsulation and sustained release of antibacterial agents through the host-guest interaction between cyclodextrin and chlorhexidine gluconate. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 Schematic diagram of the mechanism of the hydrogel prepared by the present invention for antibacterial treatment of skin surface. DETAILED DESCRIPTION

[0047] The preferred embodiments of the present invention are described below. It should be understood that the embodiments are for better explaining the present invention and are not used to limit the present invention.

[0048] Test method:

[0049] 1. Mechanical properties:

[0050] The 1 mm thick hydrogel was cut into dumbbell-shaped tensile test samples of 4*50 mm and tested using a universal testing machine at a speed of 100 mm / min.

[0051] 2. Moisturizing properties:

[0052] A disc-shaped hydrogel sample with a diameter of 1 cm and a thickness of 1 mm was placed in a constant temperature and humidity environment (25° C., 70% RH), and its weight was measured regularly.

[0053] 3. Cytotoxicity:

[0054] The sample was mixed with MES buffer at a mass ratio of 1:5 to extract the extract, and the cytotoxicity of the extract was characterized by the MTT method.

[0055] 4. Antibacterial properties:

[0056] Escherichia coli (10 4 cfu / mL) and Staphylococcus aureus (10 4 cfu / mL) as working bacterial solution, take 0.1mL of working bacterial solution to 5mL of test sample and mix well, take 1.0mL of mixed solution to 9mL of diluent at the test time, dilute in sequence, take 1mL to a sterile plate in parallel and then pour the culture medium, culture at 35±2℃ for 48h to count the viable bacteria, and calculate the antibacterial rate of the sample with the following formula:

[0057] X=(A–B) / A×100%

[0058] Wherein, X: inhibition rate, %; A: average colony count of control samples; B: average colony count of test samples.

[0059] Evaluation criteria:

[0060] If the antibacterial rate is ≥50-90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has a strong antibacterial effect.

[0061] 5. Long-term antibacterial effect:

[0062] After rubbing the arm evenly, take a test sample (5cm*5cm) and stick it on the divided arm surface. Take the sample at intervals from 0h to 48h. Use a sterile cotton swab to take standardized samples at the arm patch. Then dilute and pour the parallel sampling liquid for culture. After culture at 37±2℃ for 24h, count and calculate the long-term antibacterial effect of the test sample.

[0063] The raw materials used in the embodiment:

[0064] Hyaluronic acid: 40 kDa-4000 kDa, purchased from Bloomage Biotech Co., Ltd.;

[0065] Chlorhexidine gluconate (CHG) aqueous solution: 20% (w / v, g / mL), purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0066] Benzalkonium chloride and benzethonium chloride: purchased from Lonza (China) Investment Co., Ltd.;

[0067] Photoinitiator 2959: purity 98%, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0068] The preparation method of acryloyl cyclodextrin (Ac-βCD) is as follows:

[0069] 1 g of 6-amino-β-cyclodextrin was dissolved in 50 mL of 0.2 M sodium bicarbonate aqueous solution, and the pH of the solution was adjusted to 10 with 0.1 M NaOH; 96 mg of acryloyl chloride was added under ice bath conditions and reacted for 10 hours; after the reaction, the solution was concentrated, precipitated with acetone, filtered, washed and collected to obtain acryloyl cyclodextrin (Ac-βCD).

[0070] The preparation method of adamantane-hyaluronic acid (Ad-HA) is as follows:

[0071] Dissolve 1 g of hyaluronic acid (HA) in 90 mL of deionized water to obtain a hyaluronic acid aqueous solution;

[0072] 610 mg of 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC), 50 mg of 1-hydroxybenzotriazole (HOBt) and 90 mg of 1-adamantanemethane were dissolved in 250 mL of dimethyl sulfoxide (DMSO) to obtain a mixed solution;

[0073] The mixed solution and the hyaluronic acid aqueous solution were mixed and reacted at 45° C. for 24 hours. After the reaction, acetone was used for precipitation, and the precipitate was washed and collected after filtration to obtain adamantane-hyaluronic acid (Ad-HA).

[0074] The w / v involved in the examples is g / mL, and % is the ratio of a substance to the solvent (water).

[0075] Example 1

[0076] A method for preparing a hydrogel for antibacterial treatment of skin surface comprises the following steps:

[0077] (1) dispersing acryloyl cyclodextrin (Ac-βCD) in water to obtain a 4% w / v aqueous solution I;

[0078] (2) dispersing adamantane-hyaluronic acid (Ad-HA), chlorhexidine gluconate (CHG) aqueous solution, a humectant and a photoinitiator 2959 in water to obtain an aqueous solution II; wherein the concentration of adamantane-hyaluronic acid (Ad-HA) is 4% w / v; the concentration of the humectant is 5% w / v; the concentration of the photoinitiator 2959 is 0.5% w / v; and the concentration of the chlorhexidine gluconate (CHG) is 0.2% w / v;

[0079] (3) Aqueous solution I and aqueous solution II were mixed in a volume ratio of 1:1, allowed to stand at 25°C (normal temperature) for 10 min, and irradiated with 365 nm ultraviolet light for 30 min to obtain a hydrogel for antibacterial treatment of the skin surface.

[0080] Example 2

[0081] A method for preparing a hydrogel for antibacterial treatment of skin surface comprises the following steps:

[0082] (1) dispersing acryloyl cyclodextrin (Ac-βCD) in water to obtain a 2% w / v aqueous solution I;

[0083] (2) dispersing adamantane-hyaluronic acid (Ad-HA), chlorhexidine gluconate (CHG) aqueous solution, a humectant and a photoinitiator 2959 in water to obtain an aqueous solution II; wherein the concentration of adamantane-hyaluronic acid (Ad-HA) is 2% w / v; the concentration of the humectant is 5% w / v; the concentration of the photoinitiator 2959 is 0.5% w / v; and the concentration of the chlorhexidine gluconate (CHG) is 0.2% w / v;

[0084] (3) Aqueous solution I and aqueous solution II were mixed in a volume ratio of 1:1, allowed to stand at 25°C (normal temperature) for 10 min, and irradiated with 365 nm ultraviolet light for 30 min to obtain a hydrogel for antibacterial treatment of the skin surface.

[0085] Example 3

[0086] The concentration of chlorhexidine gluconate (CHG) in step (2) of Example 1 was adjusted to 1% w / v, and the other conditions were kept consistent with Example 1 to obtain a hydrogel for antibacterial treatment of the skin surface.

[0087] Example 4

[0088] The concentration of chlorhexidine gluconate (CHG) in step (2) of Example 1 was adjusted to 2% w / v, and the other conditions were kept consistent with Example 1 to obtain a hydrogel for antibacterial treatment of the skin surface.

[0089] Example 5

[0090] The glycerol in step (2) of Example 1 was adjusted to propylene glycol, and the other parts were kept consistent with Example 1 to obtain a hydrogel for antibacterial treatment of the skin surface.

[0091] Example 6

[0092] The ultraviolet irradiation time in step (3) of Example 4 was adjusted to 20 min, and the other conditions were kept consistent with Example 4 to obtain a hydrogel for antibacterial treatment of the skin surface.

[0093] Example 7

[0094] The chlorhexidine gluconate (CHG) in step (2) of Example 1 was adjusted to benzalkonium chloride, and the other steps were kept consistent with Example 1 to obtain a hydrogel.

[0095] Comparative Example 1

[0096] The glycerol and chlorhexidine gluconate (CHG) in step (2) of Example 1 were omitted, and the other steps were the same as those of Example 1 to obtain a hydrogel.

[0097] Comparative Example 2

[0098] Chlorhexidine gluconate (CHG) in step (2) of Example 1 was omitted, and the rest was kept the same as in Example 1 to obtain a hydrogel.

[0099] Comparative Example 3

[0100] The glycerol in step (2) of Example 1 was omitted, and the other steps were the same as those of Example 1 to obtain a hydrogel.

[0101] Comparative Example 4

[0102] A method for preparing a hydrogel for antibacterial treatment of skin surface comprises the following steps:

[0103] Acryloyl cyclodextrin (Ac-βCD), adamantane-hyaluronic acid (Ad-HA), chlorhexidine gluconate (CHG) aqueous solution, moisturizer and photoinitiator 2959 were dispersed in water, allowed to stand at 25°C (normal temperature) for 10 min, and irradiated with 365 nm ultraviolet light for 30 min to obtain a hydrogel;

[0104] The concentration of acryloyl cyclodextrin (Ac-βCD) is 2% w / v, the concentration of adamantane-hyaluronic acid (Ad-HA) is 2% w / v; the concentration of moisturizer is 2.5% w / v; the concentration of photoinitiator 2959 is 0.25% w / v, and the concentration of chlorhexidine gluconate (CHG) is 0.2% w / v.

[0105] Comparative Example 5

[0106] The concentration of chlorhexidine gluconate (CHG) in step (2) of Example 1 was adjusted to 3% w / v, and the other conditions were kept consistent with Example 1 to obtain a hydrogel.

[0107] Comparative Example 6

[0108] The ultraviolet irradiation in step (3) of Example 1 was adjusted to irradiation with 280 nm ultraviolet light for 30 min, and the rest was kept the same as Example 1 to obtain a hydrogel.

[0109] Comparative Example 7

[0110] The acryloyl cyclodextrin (Ac-βCD) in step (1) of Example 1 was adjusted to cyclodextrin, the adamantane-hyaluronic acid in step (2) was adjusted to ordinary hyaluronic acid, and the rest was kept the same as in Example 1 to obtain a hydrogel.

[0111] The hydrogels obtained in the examples and comparative examples were subjected to performance tests, and the test results are as follows:

[0112] (1) Mechanical properties:

[0113] Table 1 shows the test results of mechanical properties. It can be seen from Table 1 that Examples 1-7 have good mechanical properties, with elongation of 180-300% and tensile strength of 60-100 kPa.

[0114] Table 1

[0115]

[0116] (2) Moisturizing properties:

[0117] Table 2 shows the test results of the moisturizing performance. It can be seen from Table 2 that the samples of Examples 1-7 can maintain more than 80% of their own weight after 24 hours, while the samples of Comparative Examples 1 and 3 are dry after 24 hours.

[0118] Table 2

[0119]

[0120] (3) Cytotoxicity:

[0121] The samples of Examples 1-7 and Comparative Examples 1-4, 6, and 7 all have good cell compatibility, while the cell compatibility of Comparative Example 5 is relatively poor.

[0122] (4) Antibacterial properties:

[0123] Table 3 shows the test results of the antibacterial rate. It can be seen from Table 3 that the antibacterial rates of Examples 1-7 for Escherichia coli and Staphylococcus aureus are above 98%; the antibacterial rates of Comparative Examples 1 and 2 are lower than 40%.

[0124] Table 3

[0125]

[0126] (5) Long-term antibacterial effect:

[0127] Table 4 shows the long-term antibacterial test results. It can be seen from Table 4 that Examples 1-7 can achieve long-term antibacterial effects on Escherichia coli and Staphylococcus aureus; Comparative Examples 1 and 2 fail quickly, Comparative Example 3 fails after 12 hours, and Comparative Examples 4 and 6 fail after 6 hours.

[0128] Table 4

[0129]

[0130] Note: “-” represents no microbial growth; “*” represents bacterial colony count <5; “**” represents bacterial colony count <10; “***” represents bacterial colony count ≥10 or colony agglomeration.

[0131] Although the present invention has been disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for preparing a hydrogel for antibacterial treatment of skin surface, characterized in that: The steps include: (1) dispersing acryloyl cyclodextrin Ac-βCD in water to obtain aqueous solution I; wherein the concentration of aqueous solution I is 2-4% g / mL; (2) Dispersing adamantane-hyaluronic acid Ad-HA, an antibacterial agent, a moisturizing agent and a photoinitiator in water to obtain an aqueous solution II; wherein the concentration of adamantane-hyaluronic acid Ad-HA in the aqueous solution II is 2-4% g / mL; the concentration of the moisturizing agent is 4-6% g / mL; the concentration of the photoinitiator is 0.4-0.6% g / mL; the concentration of the antibacterial agent is 0.2-2% g / mL; the antibacterial agent is a cationic antibacterial agent, which is one or more of chlorhexidine gluconate CHG, benzalkonium chloride, and benzethonium chloride; the moisturizing agent is one or more of glycerol, propylene glycol, and butylene glycol; (3) Mixing aqueous solution I and aqueous solution II, allowing them to stand, and irradiating them with ultraviolet light to obtain a hydrogel for antibacterial treatment of the skin surface; wherein the volume ratio of aqueous solution I to aqueous solution II is 1:5-5:1; and the ultraviolet light irradiation is performed at a wavelength of 365 nm for 5-30 minutes.

2. The method according to claim 1, characterized in that The photoinitiator in step (2) is photoinitiator 2959.

3. The method according to claim 1, characterized in that The step (3) is to stand at 20-30°C for 8-12 minutes.

4. The method according to claim 1, characterized in that: The preparation method of acryloyl cyclodextrin Ac-βCD in step (1) is: 6-amino-β-cyclodextrin was dissolved in water and modified by acryloyl chloride to obtain acryloyl cyclodextrin Ac-βCD.

5. The method according to claim 1, characterized in that: The preparation method of adamantane-hyaluronic acid Ad-HA in step (2) is: Hyaluronic acid is dissolved in water, and adamantane is grafted onto the hyaluronic acid through a carbodiimide coupling reaction to obtain adamantane-hyaluronic acid Ad-HA; wherein the molecular weight of the hyaluronic acid is 40kDa-4000kDa.

6. The method according to claim 4, characterized in that The preparation method of acryloyl cyclodextrin Ac-βCD in step (1) is specifically as follows: 1 g of 6-amino-β-cyclodextrin was dissolved in an alkaline solution, the pH was adjusted to 10, acryloyl chloride was added in an ice bath to react, after the reaction was completed, the solution was concentrated, precipitated with acetone, filtered, washed and dried to obtain acryloyl cyclodextrin Ac-βCD; Among them, the mass ratio of 6-amino-β-cyclodextrin and acryloyl chloride is 1 g:96 mg; The alkaline solution is an aqueous solution of sodium bicarbonate; The reaction time is 10 h.

7. A hydrogel for skin surface antibacterial prepared by the method according to any one of claims 1 to 6.

8. Use of the hydrogel for antibacterial treatment of skin surface according to claim 7 in the field of wound dressing or cleaning agent.

9. A hand washing gel, characterized in that: The hydrogel for antibacterial on skin surface as claimed in claim 7 is used.

10. A skin antibacterial agent, characterized in that: The hydrogel for antibacterial on skin surface as claimed in claim 7 is used.

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