Pantoea ananatis strain yn-tcw-7 and applications thereof

Abstract

This invention discloses a strain of Pantothecinus pineapple, YN-TCW-7, and its applications, belonging to the field of microbial technology. The strain was deposited on June 28, 2024, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 31117. This bacterium and its fermentation products exhibit significant inhibitory effects on root and stem pathogens of leguminous crops and tobacco, including *Phytophthora indicum*, *Phytophthora sclerotiorum*, *Phytophthora radiata*, *Phytophthora causal agent*, *Phytophthora causal agent*, *Phytophthora radiata*, *Phytophthora causal agent*, *Phytophthora radiata*, *Phytophthora radiata*, *Phytophthora radiata*, and *Phytophthora fusarium*, thus playing an important role in the prevention and control of microbial diseases in leguminous crops and tobacco.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a strain of Pantotheca pineapple YN-TCW-7 and its applications. Background Technology

[0002] Legumes and tobacco are important oilseed and cash crops. In recent years, due to climate warming, the promotion of high-yield legume varieties, and changes in farming practices, especially the promotion of dense planting, significant changes have occurred in field microclimates and soil microecologies, leading to increasingly severe soil-borne and foliar diseases in legumes and tobacco. Fungal diseases, due to the large number of pathogens, significant regional differences, and the continuous reporting of new pathogens in various regions, coupled with the lack of effective control methods in production, have become the most important diseases affecting legume and tobacco production.

[0003] The main pathogens affecting leguminous crop production include soybean root rot fungus (Fusarium graminearum), peanut white mold fungus (Sclerotium rolfsii), peanut root rot fungus (Fusarium solani), peanut black rot fungus (Calonectria ilicicola), and peanut stem rot fungus (Lasiodiplodia pseudotheobromae). Once these diseases occur, they are difficult to control, and severe outbreaks can cause significant yield reductions.

[0004] Tobacco Fusarium root rot is a soil-borne root and stem disease that seriously affects tobacco production. It is primarily caused by *Fusarium oxysporum* and occurs from the seedling stage to harvest, damaging the vascular bundles of the roots and leading to wilting and death of the tobacco plants. In recent years, the affected area has been continuously expanding in various tobacco-growing regions, with increasing damage and greater difficulty in control. Tobacco red spot disease, caused by *Alternaria alternate*, is an important disease in the middle and late stages of tobacco growth, occurring in all major tobacco-growing regions and becoming the second most common tobacco disease after viral diseases.

[0005] Currently, the control of pests and diseases in legumes and tobacco mainly relies on resistant plant varieties and chemical control. However, the problem of pest resistance is becoming increasingly serious, and chemical pesticide residues cause serious harm, such as toxicity and environmental pollution, posing a significant threat to human health and the ecosystem, and hindering the sustainable development of the crop cultivation industry. Therefore, finding an economical, safe, and effective control measure is urgently needed. Biological control measures utilize beneficial microorganisms to kill or reduce the number of pathogens to control the occurrence and development of plant diseases. Discovering and creating highly efficient biological agents is an important measure for the safe and effective control of such diseases. Summary of the Invention

[0006] This invention provides a strain of Pantoea ananatis YN-TCW-7, which was deposited at the China General Microbiological Culture Collection Center on June 28, 2024, with accession number CGMCCNo.31117.

[0007] The aforementioned Pantothecin YN-TCW-7 pineapple strain exhibits significant inhibitory effects on pathogens affecting legumes and tobacco roots and stems, including soybean root rot fungus, peanut white mold fungus, peanut root rot fungus, peanut black rot fungus, peanut stem rot fungus, tobacco red spot fungus, and tobacco falciparum fungus.

[0008] This invention provides the application of the above-mentioned Pantothecin pineapple YN-TCW-7 in the preparation of agents that inhibit plant pathogens; the plant is selected from legumes and / or tobacco; the legume is selected from peanuts and / or soybeans; the pathogen is selected from one or more of the following: Soybean root rot fungus, Peanut white mold fungus, Peanut root rot fungus, Peanut black rot fungus, Peanut stem rot fungus, Tobacco red spot fungus, and Tobacco Fusarium wilt fungus.

[0009] This invention provides the application of the above-mentioned Pantothecin pineapple YN-TCW-7 in the preparation of formulations that promote plant growth.

[0010] This invention provides the application of the above-mentioned Pantotheca pineapple YN-TCW-7 in the preparation of formulations that promote plant growth while simultaneously inhibiting plant pathogens.

[0011] This invention provides a microbial preparation containing the above-mentioned Pantothecin pineapple YN-TCW-7.

[0012] In one specific embodiment, the microbial preparation is prepared by the following method:

[0013] The activated Pantotheca pineapple YN-TCW-7 was inoculated into LB liquid medium and cultured in a shaker at 28°C with shaking at 180 rpm until the bacterial suspension concentration reached 10. 7 CFU / mL was used to obtain the microbial preparation.

[0014] The above LB liquid culture medium formula is as follows:

[0015] 10g tryptone, 5g yeast extract, 10g sodium chloride, 1000mL distilled water, adjust pH to 7.0, autoclave at 121℃ for 20min.

[0016] The beneficial effects of this invention are as follows:

[0017] This invention provides a pineapple pancreatobacterium, which and its fermentation products have a significant inhibitory effect on root and stem pathogens of leguminous crops and tobacco, including soybean root rot fungus, peanut white mold fungus, peanut root rot fungus, peanut black rot fungus, peanut stem rot fungus, tobacco red spot fungus, and tobacco falciparum fungus. Therefore, it is of great significance for the prevention and control of microbial diseases of leguminous crops and tobacco. Attached Figure Description

[0018] Figure 1 The colony morphology of strain YN-TCW-7 on LB medium;

[0019] Figure 2 This is a 16S rDNA gel electrophoresis image of strain YN-TCW-7.

[0020] Figure 3 An evolutionary tree was constructed for strain YN-TCW-7 and other bacteria;

[0021] Figure 4 The inhibitory effect of strain YN-TCW-7 on soybean root rot pathogen;

[0022] Figure 5 The inhibitory effect of strain YN-TCW-7 on peanut white rot fungus;

[0023] Figure 6 The inhibitory effect of strain YN-TCW-7 on peanut root rot pathogen;

[0024] Figure 7 The inhibitory effect of strain YN-TCW-7 on peanut black rot fungus;

[0025] Figure 8 The inhibitory effect of strain YN-TCW-7 on peanut stem rot pathogen;

[0026] Figure 9 The inhibitory effect of strain YN-TCW-7 on Tobacco Aster spp. was investigated.

[0027] Figure 10 The inhibitory effect of strain YN-TCW-7 on tobacco fusarium wilt;

[0028] Figure 11 The effect of strain YN-TCW-7 on the growth promotion of potted peanuts;

[0029] Figure 12 The effect of strain YN-TCW-7 on the growth promotion of potted soybeans;

[0030] Figure 13 The effect of strain YN-TCW-7 on the growth promotion of peanuts in the field;

[0031] Figure 14The effect of strain YN-TCW-7 on promoting the growth of soybeans in the field. Detailed Implementation

[0032] Strain isolation and identification:

[0033] Marigold plants were sourced from robust leaves and roots of marigold plants from Tengchong, Yunnan. After thorough washing, the plants were blotted dry with filter paper, soaked in 75% ethanol for 2-3 minutes, rinsed three times with sterile water, soaked in 0.2% mercuric chloride for 3 minutes, and rinsed three times with sterile water. The mixture was then ground into a slurry and serially diluted 10 times. -1 -10 -6 Using a micropipette, 50 μL of homogenate at different dilutions was pipetted onto LB agar plates. The plates were then spread evenly using a sterile spreader, sealed, and incubated upside down at 28°C for 48 hours. A batch of single colonies from the plates was picked, streaked onto LB agar plates, and incubated upside down at 28°C for 1-2 days to obtain a bacterial strain. This strain was designated YN-TCW-7.

[0034] Following the methods described in Bergey's Manual of Bacterial Identification (8th Edition), strain YN-TCP-8 was identified by morphological and physiological-biochemical characteristics. The specific results are as follows:

[0035] 1. Morphological characteristics

[0036] The colonies are round, pale yellow, opaque, viscous, and have neat edges, like... Figure 1 As shown, microscopic observation of the bacterial cell morphology reveals that it is a Gram-negative, rod-shaped bacterium.

[0037] 2. Biological characteristics

[0038] The strain YN-TCW-7 is Gram-negative and VP-positive. It can hydrolyze gum and citrate, but not starch. It can utilize sugars such as lactose, glucose, and sucrose.

[0039] The results of physiological and biochemical identification are shown in Table 1:

[0040] Table 1. Physiological and biochemical characteristics of active strain YN-TCW-7

[0041] Gram staining - Mingjiao hydrolysis + VP Measurement + Starch hydrolysis - citrate + maltose + lactose + sucrose +

[0042] Note: "+" indicates a positive result; "-" indicates a negative result.

[0043] 3. Genetic characteristics

[0044] Three identical colonies were selected for genomic DNA extraction. The extraction method was mainly performed according to the instructions of the TIANGENTIAN amp BACTERia DNA Kit, and the steps are as follows:

[0045] (1) Take 1 mL of bacterial culture medium, centrifuge at 10,000 rpm for 1 min, and aspirate the supernatant as much as possible;

[0046] (2) Add 200 μL of buffer GA to the bacterial pellet and shake until the bacterial pellet is completely suspended;

[0047] (3) Add 4 μL of RNAase (100 mg / mL) solution, shake for 15 seconds, and let stand at room temperature for 5 minutes;

[0048] (4) Add 20 μL of proteinase K solution to the tube and mix well;

[0049] (5) Add 220 μL buffer GB, shake for 15 seconds, place at 70°C for 10 minutes, and centrifuge to remove water droplets from the inner wall of the tube cap.

[0050] (6) Add 220 μL of anhydrous ethanol, shake well for 15 seconds. At this time, flocculent precipitate may appear. Briefly centrifuge to remove water droplets from the inner wall of the tube cap.

[0051] (7) Add the solution and flocculent precipitate obtained in the previous step to an adsorption column CB3 (place the adsorption column in the collection tube), centrifuge at 12000 rpm for 30s, discard the waste liquid and place the adsorption column CB3 into the collection tube.

[0052] (8) Add 500 μL of buffer GD to the adsorption column CB3 (check that anhydrous ethanol has been added before use), centrifuge at 12000 rpm for 30 s, discard the waste liquid and put the adsorption column CB3 into the collection tube.

[0053] (9) Add 700 μL of washing solution PW to the adsorption column CB3 (check that anhydrous ethanol has been added before use), centrifuge at 12000 rpm for 30 s, discard the waste liquid and put the adsorption column CB3 into the collection tube.

[0054] (10) Add 500 μL of washing solution PW to the adsorption column CB3, centrifuge at 12000 rpm for 30 s, discard the waste liquid and put the adsorption column CB3 into the collection tube.

[0055] (11) Put the adsorption column CB3 back into the collection tube, centrifuge at 12000 rpm for 2 min, discard the waste liquid, and place the adsorption column CB3 at room temperature for several minutes to thoroughly dry the residual rinsing liquid in the adsorption material.

[0056] (12) Transfer the adsorption column CB3 into a clean centrifuge tube, add 50-200 μL of elution buffer TE to the middle of the adsorption membrane, place at room temperature for 2-5 min, centrifuge at 12000 rpm for 2 min, collect the solution into a centrifuge tube, and store at -20℃.

[0057] PCR and sequencing

[0058] The extracted DNA was amplified by PCR according to the instructions of the TaKaRa 16S rDNA Bacterial Identification PCR Kit. The forward primer was 5'-AGAGTTTGATCATGGCTCAG-3' (27F), and the reverse primer was 3'-CGCTTACCTTGTTACGACTT-5' (1492R). The PCR amplification conditions were as follows: 94℃ pre-denaturation for 5 min; 94℃ denaturation for 1 min; 53℃ annealing for 1 min; 72℃ extension for 90 s; and 72℃ final extension for 5 min, for a total of 30 cycles. The PCR products were separated by 1% agarose gel electrophoresis, stained with EB, and observed under a 3UV™ Transilluminator (UVP, USA). Electrophoresis showed that the 16S rDNA fragment of strain YN-TCW-7 was approximately 1500 bp in size, which was consistent with the expected design of the kit. The amplification results are shown in the figure below. Figure 2 .

[0059] Based on pre-designed primers and the expected amplified fragment size, the target fragment was cut under UV light and bound to a marker. DNA was then recovered using the Silica Bead DNA Gel Extraction Kit. The 16S rDNA sequence was determined by Qingdao Qingke Biotechnology Co., Ltd. The sequencing results showed that the 16S rDNA fragment of strain YN-TCW-7 consisted of 1435 base pairs, and its nucleic acid sequence is as follows:

[0060] 16S rDNA:

[0061]

[0062] The determined sequence was compared with sequences in GenBank (NCBI, http: / / blast.ncbi.nlm.nih.gov / Blast.cgi) using the BLAST program. Then, 16S rDNA sequences from species and genera closely related to the test strain were obtained from GenBank for identification. The results showed that the 16S rDNA sequence of this active strain was highly homologous (100%) to the 16S rDNA sequence of *Pantoea ananatis*, a *Bacillus* species in the GenBank gene bank. Developmental tree construction and homology analysis yielded the following results: Figure 3 As shown, strain YN-TCW-7 and *Pantoea ananatis* (Accession No: CP028033.1) of the genus *Pantoea* form a separate clade, with the closest evolutionary distance, reflecting their closest phylogenetic relationship. Analysis using DANMAN software revealed 100% homology between the two. Based on the results of physiological and biochemical characterization and 16S rDNA sequence analysis, strain YN-TCW-7 was identified as *Pantoea ananatis*.

[0063] This strain was deposited on June 28, 2024, at the China General Microbiological Culture Collection Center (CGMCC, address: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100101, China), with accession number CGMCC No. 31117.

[0064] Other materials used in this invention, unless otherwise stated, are commercially available. Other terms used in this invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art. The invention is further described in detail below with reference to specific embodiments and data. The following embodiments are merely illustrative and not intended to limit the scope of the invention in any way.

[0065] Example 1

[0066] Antibacterial function test:

[0067] Single colonies of YN-TCW-7 were picked and inoculated into LB medium and cultured at 28°C and 200 rpm for 12 h. 100 μL of the culture was then transferred to 20 mL of LB medium and cultured in a shaker at 28°C and 180 rpm until the bacterial suspension concentration reached 10⁻⁶. 6cfu / mL. In a sterile operating table, inoculate a 0.5 cm diameter pathogenic fungal disc onto a PDA agar plate, add 6 μL of LYN-TCW-7 culture medium at a distance of 2.5 cm from the disc, and incubate upside down in a 28°C incubator for 10-15 days.

[0068] The pathogens tested were *Fusarium graminearum*, *Sclerotium rolfsii*, *Fusarium solani*, *Calonectriailicicola*, *Lasiodiplodia pseudotheobromae*, *Alternaria alternate*, and *Fusarium oxysporum*.

[0069] Formula for calculating antibacterial rate:

[0070] Inhibition rate (%) = (Control colony diameter - Treated colony diameter) / Control colony diameter × 100

[0071] The test results are as follows Figures 4-10 As shown, Pantothecin pineapple YN-TCW-7 has a good inhibitory effect on various pathogens, with an inhibition rate of over 50%.

[0072] Example 2

[0073] Preparation of fermentation preparations:

[0074] Single colonies of strain YN-TCW-7 were streaked onto LB agar plates and incubated upside down at 28°C for 1-2 days. The activated strain YN-TCW-7 was then inoculated onto LB liquid medium and cultured at 180 rpm for 3 days at 28°C until the bacterial suspension concentration reached 10⁻⁶. 7 cfu / mL.

[0075] The above LB solid culture medium formula is as follows:

[0076] 10g tryptone, 5g yeast extract, 10g sodium chloride, 15g agar powder, 1000mL distilled water, adjust pH to 7.0, autoclave at 121℃ for 20min.

[0077] The above LB liquid culture medium formula is as follows:

[0078] 10g tryptone, 5g yeast extract, 10g sodium chloride, 1000mL distilled water, adjust pH to 7.0, autoclave at 121℃ for 20min.

[0079] Example 3

[0080] 1. Experiment on the control of flowering plant diseases by strain YN-TCW-7 in indoor potted plants

[0081] After activation, the strain was inoculated into LB liquid medium and cultured in a shaker at 28°C for 180 rpm for 3 days to achieve a bacterial suspension concentration of 10. 7 cfu / mL.

[0082] Propagation of preserved pathogens of peanut white mold and peanut root rot: Place oat grains in Erlenmeyer flasks, soak in distilled water for 6 hours, drain the water, and autoclave at 121℃ for 20 minutes; inoculate peanut white mold and peanut root rot pathogens into sterilized oat grains respectively, and incubate at 28℃ for 7 days, shaking the inoculation flasks twice a day to ensure that all oat grains have pathogens, thus obtaining infected oat grains.

[0083] Pathogen inoculation: Peanuts (Huayu 36) were planted in pots in an artificial climate chamber. The mass ratio of soil:vermiculite:nutrient soil was 2:1:1. The cultivation temperature was 28℃ during the day and 25℃ at night, with a 12-hour light / 12-hour dark cycle. Peanuts were planted individually, with 3 seeds per pot. Ten seeds of each pathogen were inoculated around the peanut plants using the topsoil spreading method, followed by covering with a thin layer of soil and watering.

[0084] YN-TCW-7 inoculation: YN-TCW-7 fermentation liquid was applied to the roots at the sowing period and 10 days after sowing, with 10 pots per treatment, 3 plants per pot, and replicated 3 times. Each pot was irrigated with 100 mL of a 100-fold diluted fermentation liquid each time. Two controls were set up: 800-fold diluted 50% carbendazim solution and water. Peanut disease incidence was observed daily. After 75 days of planting, the incidence of peanut white mold and peanut root rot was investigated. Peanuts were watered once daily.

[0085] Peanut white mold disease grading standards: Grade 0: No symptoms in the plant; Grade 1: Lesions only occur at the base of the stem; Grade 2: Constriction symptoms appear at the base of the stem, and the infected parts showing systemic symptoms (wilt, death, wilting, etc.) account for less than one-third of the whole plant; Grade 3: The infected parts showing systemic symptoms account for less than two-thirds of the whole plant; Grade 4: The infected parts showing systemic symptoms account for more than two-thirds of the whole plant.

[0086] Peanut root rot grading standards: Grade 0: No lesions on the stem base and main root; Grade 1: A small number of lesions on the stem base and main root; Grade 3: Many lesions on the stem base and main root, with the lesion area accounting for 1 / 4 to 1 / 2 of the total area of ​​the stem base and root; Grade 5: Many and large lesions on the stem base and main root, with the lesion area accounting for 1 / 2 to 3 / 4 of the total area of ​​the stem base and root; Grade 7: Lesions on the stem base and main root merge together, forming a phenomenon of wrapping around the stem, but the root system is not dead; Grade 9: Root necrosis, and the above-ground parts of the plant wilt or die.

[0087] Disease incidence rate = (Number of diseased plants / Total number of plants) × 100%

[0088] Disease index = ∑(Representative value of disease severity level × Number of diseased plants at each level) × 100 / (Total number of plants surveyed × Representative value of the highest disease severity level)

[0089] Prevention and control effect = [(Control disease index - Treatment disease index) / Control disease index] × 100%

[0090] The results of the pot experiment are shown in Tables 2 and 3:

[0091] Table 2. Control effect of strain YN-TCW-7 on peanut white mold in potted plants.

[0092]

[0093] Table 3. Control effect of strain YN-TCW-7 on peanut root rot in potted plants.

[0094]

[0095] Peanut strains were treated with the fermentation broth of biocontrol fungus YN-TCW-7. YN-TCW-7 showed a 48.5% control effect on white mold disease in indoor potted peanuts (Table 2); and a 48.6% control effect on root rot disease in indoor potted peanuts (Table 3), comparable to the control fungicide carbendazim. This indicates that biocontrol fungus YN-TCW-7 has significant control effects on both white mold disease and root rot disease in peanuts.

[0096] 2. Field test of strain YN-TCW-7 for the control of peanut white mold and root rot

[0097] The experiment was conducted at the Laixi Experimental Farm of the Shandong Peanut Research Institute, in a field where peanuts had been continuously cropped, resulting in severe outbreaks of peanut white mold and root rot. A randomized block design was used, with each peanut plot measuring 70m². 2 Four replicates were performed. Water served as a blank control, and 50% carbendazim at 800 times dilution served as a positive control. YN-TCW-7 fermentation broth was applied to the roots at sowing time and 10 days after sowing, with approximately 100 mL of a 100-fold diluted fermentation broth applied to each plant each time. Other field management was the same as normal production. Disease incidence was assessed after 75 days.

[0098] The results of the field trials are shown in Tables 4 and 5:

[0099] Table 4. Field application efficacy of strain YN-TCW-7 against peanut white mold disease.

[0100]

[0101] Table 5. Field application efficacy of strain YN-TCW-7 against peanut root rot.

[0102]

[0103] The control efficacy of YN-TCW-7 against peanut white mold was 41.2% (see Table 4); the control efficacy against peanut root rot was 40.0% (see Table 5). This indicates that the biocontrol agent YN-TCW-7 has significant control effects on both peanut white mold and root rot, and its control efficacy against these diseases is basically equivalent to that of carbendazim treatment.

[0104] In summary, treatment of peanut strains with the fermentation broth of biocontrol bacteria YN-TCW-7 showed significant control effects against peanut white mold and root rot in both indoor pot and field trials.

[0105] Example 4

[0106] 1. Experiment on the growth-promoting effect of strain YN-TCW-7 in indoor potted plants

[0107] Peanut (Huayu 36) and soybean (Zhonghuang 37) were irrigated with YN-TCW-7 fermentation liquid at the sowing period and 10 days after sowing, with 10 pots of 3 plants per pot for each treatment, and replicated 3 times. Each pot was irrigated with 100 mL of 100-fold diluted fermentation liquid, with the same volume of water used as a control. Peanuts / soybeans were harvested 4 months after sowing. The potted peanuts / soybeans were removed, and growth indicators such as main stem height, lateral branch length, diameter at ground level, and number of branches were measured. The number of pods was recorded. The pods were washed with running water to remove soil particles, air-dried, and weighed. The above-ground and underground parts of the peanut / soybean plants were cut off with scissors, placed in a drying oven at 80℃ to constant weight, and then weighed.

[0108] The test results are as follows Figures 11-12 And as shown in Tables 6 and 7:

[0109] Table 6. Effects of YN-TCW-7 on the growth and development of potted peanuts

[0110]

[0111] Table 7 Effects of YN-TCW-7 on the growth and development of potted soybeans

[0112]

[0113] Peanuts and soybeans treated with YN-TCW-7 showed significantly higher growth indicators than the control group, indicating that YN-TCW-7 significantly promoted the growth and development of peanuts and soybeans and increased their yield.

[0114] 2. Field yield-increasing effect test of strain YN-TCW-7

[0115] The experiment was conducted at the Laixi Experimental Farm of the Shandong Peanut Research Institute, using a randomized block design with 70m² of peanut / soybean plots. 2 The experiment was repeated four times, with water serving as a blank control. YN-TCW-7 fermentation solution was applied to the roots at sowing time and 10 days after sowing, with approximately 100 mL of a 100-fold diluted fermentation solution applied per clump each time. Other field management was the same as normal production. Each plot yielded 12 m² of harvest. 2 The survey investigated peanut and soybean production.

[0116] The test results are as follows Figures 13-14 And as shown in Tables 8 and 9:

[0117] Table 8. Effects of YN-TCW-7 on peanut yield in the field.

[0118]

[0119]

[0120] Table 9. Effects of YN-TCW-7 on soybean yield in field fields.

[0121]

[0122] Peanut yields treated with YN-TCW-7 inoculant increased by 21.7% compared to the control, while soybean yields treated with YN-TCW-7 inoculant increased by 8.7% compared to the control. This indicates that strain YN-TCW-7 has a good yield-increasing effect on both peanuts and soybeans.

[0123] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A pineapple plant with Pantothecin ( Pantoea ananatis YN-TCW-7, characterized in that, The strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on June 28, 2024, with accession number CGMCC No. 31117.

2. The use of the Pantothecin pineapple YN-TCW-7 of claim 1 in the preparation of a formulation that promotes plant growth while simultaneously inhibiting plant pathogens; The plant is selected from legumes and / or tobacco; the legume is selected from peanuts and / or soybeans; The pathogen was selected from soybean root rot fungus. Fusarium graminearum Peanut white mold fungus Sclerotium rolfsii Peanut root rot pathogen Fusarium solani Peanut black rot fungus Calonectria ilicicola Peanut stem rot fungus Lasiodiplodia pseudotheobromae Tobacco red spot bacterium Alternaria alternate Tobacco Fusarium Fusarium oxysporum One or more of them.

3. A microbial preparation, characterized in that, The microbial preparation contains Pantothecin pineapple YN-TCW-7 as described in claim 1.

4. The microbial preparation according to claim 3, characterized in that, The microbial preparation is prepared by the following method: The activated Pantotheca pineapple YN-TCW-7 was inoculated into LB liquid medium and cultured in a shaker at 28°C with shaking at 180 r / min until the bacterial suspension concentration reached 10. 7 CFU / mL was used to obtain the microbial preparation.

5. The microbial preparation according to claim 4, characterized in that, The LB liquid culture medium formulation is as follows: 10g tryptone, 5g yeast extract, 10g sodium chloride, 1000mL distilled water, adjust pH to 7.0, autoclave at 121℃ for 20min.

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