Medicinal tea for nourishing stomach, aiding digestion and resisting allergy and preparation method thereof
By using raw materials such as crape myrtle, jasmine and pomegranate flowers, combined with white tea polysaccharides and other ingredients, and using supercritical CO2 extraction and ultrasonic extraction, a medicinal tea with stomach nourishing and digestion and anti-allergic effects was prepared, which solved the problems of stomach discomfort and allergies in modern life, and achieved the improvement of the functionality and nutritional components of tea beverages.
Patent Information
- Application Number
- CN202410835307.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-26
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2044-06-26
AI Technical Summary
Modern life leads to the widespread gastric discomfort and allergies, and existing tea drinks lack effective functions to nourish the stomach and aid digestion and anti-allergic functions.
Using raw materials such as crape myrtle, jasmine and pomegranate flowers, combined with white tea polysaccharides, coix seed, monkey erectus mushrooms and other ingredients, a medicinal tea that nourishes the stomach, aids digestion and anti-allergicity through supercritical CO2 extraction, ultrasonic extraction and microwave-assisted enzymatic decomposition are prepared.
This medicinal tea can effectively inhibit gastric ulcers, enhance gastrointestinal function, significantly reduce IgE content, provide good anti-allergic effects, and at the same time improve the function and nutritional content of the tea, improve taste and flavor.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of medicinal tea preparation, in particular to a medicinal tea capable of nourishing the stomach, aiding digestion and resisting allergy and a preparation method thereof. Background Art
[0002] Fast-paced life and high-pressure work have led to many people having irregular work and rest schedules, unbalanced diets, excessive intake of high-salt, high-oil and high-fat foods, and increased stomach discomfort symptoms. The elderly have declining physical functions and aging organs, and many people often have stomach discomfort. Nowadays, many people are prone to allergies due to environmental pollution, pressure, and microbial infection, which seriously affects people's health. Therefore, the pursuit of health has become particularly important for everyone. People have begun to pursue health in different ways. People's requirements for drinking tea are no longer limited to quenching thirst. At present, there are many varieties of tea sold on the market, which play the role of clearing heat, quenching thirst, and refreshing. They only pay attention to aroma and taste, and ignore other effects. Summary of the invention
[0003] In view of this, the present invention provides a medicinal tea capable of nourishing the stomach, aiding digestion and resisting allergy and a preparation method thereof.
[0004] The technical solution of the present invention is achieved in this way:
[0005] A stomach-nourishing, digestion-aiding and anti-allergic medicinal tea comprises the following raw materials in parts by weight: 10-15 parts of crape myrtle flowers, 15-20 parts of jasmine flowers, 10-15 parts of pomegranate flowers, 3-7 parts of white tea polysaccharides, 6-10 parts of coix seeds, 3-5 parts of hericium erinaceus, 1-3 parts of tangerine peel, 2-4 parts of yam, 2-6 parts of natto dry powder, and 1-3 parts of sodium citrate.
[0006] Furthermore, the following raw materials are included in proportion by weight: 13 parts of crape myrtle flower, 18 parts of jasmine flower, 13 parts of pomegranate flower, 5 parts of white tea polysaccharide, 8 parts of coix seed, 4 parts of hericium erinaceus, 2 parts of tangerine peel, 3 parts of yam, 4 parts of natto dry powder, and 2 parts of sodium citrate.
[0007] Furthermore, the preparation steps of the white tea polysaccharide include: crushing the white tea, performing supercritical CO2 extraction, separating the extract, concentrating under reduced pressure, precipitating the concentrate with alcohol, refining and drying to obtain the white tea polysaccharide.
[0008] Furthermore, the preparation steps of the white tea polysaccharide are specifically as follows: crushing the white tea into 100-200 meshes, performing supercritical CO2 extraction for 30-60 min at a temperature of 30-40°C, a pressure of 100-200 bar, and a flow rate of 10-20 mL / min, separating the extract by centrifugation at 3000-5000 rpm for 10-20 min, concentrating under reduced pressure, precipitating the concentrate with 50-80% v / v ethanol for 30-40 min, refining, and drying at 40-60°C for 2-4 h to obtain white tea polysaccharide.
[0009] A method for preparing a medicinal tea for nourishing the stomach, aiding digestion and resisting allergies, the specific steps comprising:
[0010] S1. Wash fresh crape myrtle flowers, jasmine flowers and pomegranate flowers, precool, freeze-dry, grind into powder, add into ethanol-ammonium sulfate aqueous two-phase system, perform ultrasonic extraction, collect the extract, concentrate, spray dry, and obtain powder A;
[0011] S2, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding them to the enzyme solution, performing microwave-assisted enzymolysis, filtering the extract through an ultrafiltration membrane, concentrating the filtrate under reduced pressure, and spray drying to obtain powder B;
[0012] S3. Add powder A and powder B into water, stir ultrasonically, add white tea polysaccharide, natto dry powder and sodium citrate, continue stirring, and homogenize to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0013] Furthermore, in step S1, the precooling is performed at 0-5°C for 10-12 hours; and the ultrasonic extraction is performed at a temperature of 20-40°C and a power of 300-400W for 2-4 hours.
[0014] Furthermore, in step S1, the concentration of the ethanol-ammonium sulfate aqueous two-phase system is 30-50% v / v, wherein the volume ratio of ethanol to ammonium sulfate is 1-3:1; and the amount of the ethanol-ammonium sulfate aqueous two-phase system added is 6-8 times the total weight of the crape myrtle flower, jasmine flower and pomegranate flower.
[0015] Furthermore, in step S2, the amount of the enzyme solution added is 4-6 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam; the enzyme solution is composed of protease, amylase and cellulase in a mass ratio of 1:1-3:3-7, and the enzyme activity is 80-100 IU / mL; the microwave-assisted enzymolysis is performed at 400-600W and 50-60°C for 1-3h.
[0016] Furthermore, in step S2, the molecular weight cutoff of the ultrafiltration membrane is 2kDa-4kDa.
[0017] Furthermore, in step S3, the liquid-to-solid ratio of water to powder A and powder B is 10-20:1 mL / g; the ultrasonic stirring is at 400-600 rpm and 20-30°C for 20-40 min; the continued stirring time is 40-60 min; the homogenization speed is 2000-3000 rpm, the time is 5-10 min, and the temperature is 30-40°C.
[0018] Compared with the prior art, the present invention has the following beneficial effects:
[0019] 1. The medicinal tea prepared by the present invention helps to inhibit gastric ulcers, thereby achieving the effects of nourishing the stomach and aiding digestion and anti-allergic effects, and can comprehensively enhance the functionality of the medicinal tea and provide nutritional ingredients. The present invention uses crape myrtle flowers, jasmine flowers and pomegranate flowers as main active ingredients, and compound other raw material components. The ingredients are safe, without additives, and synergistic. The prepared medicinal tea has no bitter taste, has enhanced flavor, is more popular with consumers, and has good market value.
[0020] 2. The preparation method of the medicinal tea of the present invention adopts an ethanol-ammonium sulfate two-phase aqueous system, assisted by ultrasonic extraction and microwave-assisted enzymatic hydrolysis technology, so as to better extract the active ingredients and retain the active ingredients from being degraded. The preparation process of the present invention improves the stability and biological activity of the medicinal tea and has high practical value. DETAILED DESCRIPTION
[0021] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.
[0022] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.
[0023] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.
[0024] Example 1
[0025] A stomach-nourishing, digestion-aiding and anti-allergic medicinal tea comprises the following raw materials in parts by weight: 13 parts of crape myrtle flowers, 18 parts of jasmine flowers, 13 parts of pomegranate flowers, 5 parts of white tea polysaccharides, 8 parts of coix seeds, 4 parts of hericium erinaceus, 2 parts of tangerine peel, 3 parts of yam, 4 parts of natto dry powder, and 2 parts of sodium citrate.
[0026] The preparation method of the above-mentioned medicinal tea for nourishing the stomach, aiding digestion and resisting allergies comprises the following specific steps:
[0027] S1. Grind the white tea into 150 mesh, place it in a supercritical extraction device, set the temperature to 35°C, the pressure to 150 bar, and the CO2 flow rate to 15 mL / min, perform supercritical CO2 extraction for 45 minutes, centrifuge the extract at 4000 rpm for 15 minutes for separation, concentrate the separated liquid under reduced pressure, then use 65% v / v ethanol to precipitate the concentrated liquid for 35 minutes, further purify it, and dry it at 50°C for 3 hours to obtain white tea polysaccharides;
[0028] S2, wash fresh crape myrtle, jasmine and pomegranate flowers, precool at 3 ° C for 11 hours, freeze-dry, and grind into powder, add the powder into a 40% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 7 times the total weight of crape myrtle, jasmine and pomegranate, wherein the volume of ethanol to ammonium sulfate is 2:1, perform ultrasonic extraction at 30 ° C, 350W for 3 hours, collect the extract, concentrate the extract, and spray dry to obtain powder A;
[0029] S3, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding an enzyme solution composed of protease, amylase and cellulase in a mass ratio of 1:2:5, which is 5 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam, wherein the enzyme activities of protease, amylase and cellulase are all 90 IU / mL, and assisted enzymolysis is carried out under microwave conditions of 500 W and 55° C. for 2 h, and the extract is filtered through an ultrafiltration membrane with a molecular weight cutoff of 3 kDa, and the filtrate is concentrated under reduced pressure, and spray-dried to obtain powder B;
[0030] S4. Add powder A and powder B into water at a liquid-solid ratio of 15:1 mL / g, stir ultrasonically at 500 rpm and 25°C for 30 min, add white tea polysaccharide, natto dry powder and sodium citrate and continue stirring for 50 min, homogenize at 2500 rpm and 35°C for 8 min to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0031] Example 2
[0032] A stomach-nourishing, digestion-aiding and anti-allergic medicinal tea comprises the following raw materials in parts by weight: 10 parts of crape myrtle flowers, 15 parts of jasmine flowers, 10 parts of pomegranate flowers, 3 parts of white tea polysaccharides, 6 parts of coix seeds, 3 parts of hericium erinaceus, 1 part of tangerine peel, 2 parts of yam, 2 parts of natto dry powder, and 1 part of sodium citrate.
[0033] The preparation method of the above-mentioned medicinal tea for nourishing the stomach, aiding digestion and resisting allergies comprises the following specific steps:
[0034] S1. Grind the white tea into 100 meshes, place in a supercritical extraction device, set the temperature to 30°C, the pressure to 100 bar, the CO2 flow rate to 10 mL / min, perform supercritical CO2 extraction for 30 min, centrifuge the extract at 3000 rpm for 10 min for separation, concentrate the separated liquid under reduced pressure, then use 50% v / v ethanol to precipitate the concentrated liquid for 30 min, further purify, and dry at 40°C for 2 h to obtain white tea polysaccharides;
[0035] S2, washing fresh crape myrtle, jasmine and pomegranate flowers, precooling at 0°C for 10 h, freeze-drying, and crushing into powder, adding the powder into a 30% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 6 times the total weight of crape myrtle, jasmine and pomegranate, wherein the volume of ethanol and ammonium sulfate is 1:1, ultrasonic extraction is performed at 20°C and 300°C for 2 h, collecting the extract, concentrating the extract, and spray drying to obtain powder A;
[0036] S3, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding an enzyme solution composed of protease, amylase and cellulase in a mass ratio of 1:1:3, which is 4 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam, wherein the enzyme activities of protease, amylase and cellulase are all 80 IU / mL, and assisted enzymolysis is carried out under microwave conditions of 400 W and 50° C. for 1 h, and the extract is filtered through an ultrafiltration membrane with a molecular weight cutoff of 2 kDa, and the filtrate is concentrated under reduced pressure, and spray-dried to obtain powder B;
[0037] S4. Add powder A and powder B into water at a liquid-solid ratio of 10:1 mL / g, stir ultrasonically at 400 rpm and 20°C for 20 min, add white tea polysaccharide, natto dry powder and sodium citrate and continue stirring for 40 min, homogenize at 2000 rpm and 30°C for 5 min to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0038] Example 3
[0039] A stomach-nourishing, digestion-aiding and anti-allergic medicinal tea comprises the following raw materials in parts by weight: 15 parts of crape myrtle flowers, 20 parts of jasmine flowers, 15 parts of pomegranate flowers, 7 parts of white tea polysaccharides, 10 parts of coix seeds, 5 parts of hericium erinaceus, 3 parts of tangerine peel, 4 parts of yam, 6 parts of natto dry powder, and 3 parts of sodium citrate.
[0040] The preparation method of the above-mentioned medicinal tea for nourishing the stomach, aiding digestion and resisting allergies comprises the following specific steps:
[0041] S1. Grind the white tea into 200 meshes, place in a supercritical extraction device, set the temperature to 40°C, the pressure to 200 bar, the CO2 flow rate to 20 mL / min, perform supercritical CO2 extraction for 60 min, centrifuge the extract at 5000 rpm for 20 min for separation, concentrate the separated liquid under reduced pressure, then use 80% v / v ethanol to precipitate the concentrated liquid for 40 min, further purify, and dry at 60°C for 4 h to obtain white tea polysaccharides;
[0042] S2, wash fresh crape myrtle, jasmine and pomegranate flowers, precool at 5 ° C for 12 hours, freeze-dry, and grind into powder, add the powder into a 50% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 8 times the total weight of crape myrtle, jasmine and pomegranate, wherein the volume of ethanol to ammonium sulfate is 3:1, perform ultrasonic extraction at 40 ° C, 400 W for 4 hours, collect the extract, concentrate the extract, and spray dry to obtain powder A;
[0043] S3, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding an enzyme solution composed of protease, amylase and cellulase in a mass ratio of 1:3:7, which is 6 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam, wherein the enzyme activity of protease, amylase and cellulase is 100 IU / mL, and assisted enzymolysis is carried out under microwave conditions of 600 W and 60° C. for 3 h, and the extract is filtered through an ultrafiltration membrane with a molecular weight cutoff of 4 kDa, and the filtrate is concentrated under reduced pressure, and spray-dried to obtain powder B;
[0044] S4. Add powder A and powder B into water at a liquid-solid ratio of 20:1 mL / g, stir ultrasonically at 600 rpm and 30°C for 40 min, add white tea polysaccharide, natto dry powder and sodium citrate and continue stirring for 60 min, homogenize at 3000 rpm and 40°C for 10 min to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0045] Comparative Example 1
[0046] The difference from Example 1 is that the amount of raw materials is different, and the rest is consistent with Example 1.
[0047] A stomach-nourishing, digestion-aiding and anti-allergic medicinal tea comprises the following raw materials in parts by weight: 9 parts of crape myrtle flowers, 10 parts of jasmine flowers, 9 parts of pomegranate flowers, 2 parts of white tea polysaccharides, 11 parts of coix seeds, 6 parts of hericium erinaceus, 4 parts of tangerine peel, 5 parts of yam, 1 part of natto dry powder, and 0.8 parts of sodium citrate.
[0048] Comparative Example 2
[0049] The difference from Example 1 is that: Lagerstroemia indica is missing, and the missing raw materials are allocated to other components in proportion. The rest is consistent with Example 1.
[0050] Step S2 of the preparation method of the stomach-nourishing, digestion-aiding and anti-allergic medicinal tea is as follows: wash fresh jasmine flowers and pomegranate flowers, precool them at 3°C for 11 hours, freeze-dry them, and grind them into powder; add the powder into a 40% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 7 times the total weight of the jasmine flowers and pomegranate flowers, wherein the volume of ethanol to ammonium sulfate is 2:1; perform ultrasonic extraction at 30°C and 350W for 3 hours; collect the extract, concentrate the extract, and spray-dry to obtain powder A.
[0051] Comparative Example 3
[0052] The difference from Example 1 is that jasmine is missing, and the missing raw materials are allocated to other components in proportion. The rest is consistent with Example 1.
[0053] Step S2 of the preparation method of the stomach-nourishing, digestion-aiding and anti-allergic medicinal tea is as follows: wash fresh crape myrtle flowers and pomegranate flowers, precool at 3°C for 11 hours, freeze-dry, and grind into powder; add the powder into a 40% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 7 times the total weight of the crape myrtle flowers and pomegranate flowers, wherein the volume of ethanol to ammonium sulfate is 2:1; perform ultrasonic extraction at 30°C and 350W for 3 hours; collect the extract, concentrate the extract, and spray-dry to obtain powder A.
[0054] Comparative Example 4
[0055] The difference from Example 1 is that pomegranate flowers are missing, and the missing raw materials are allocated to other components in proportion. The rest is consistent with Example 1.
[0056] Step S2 of the preparation method of the stomach-nourishing, digestion-aiding and anti-allergic medicinal tea is as follows: wash fresh crape myrtle flowers and jasmine flowers, precool them at 3°C for 11 hours, freeze-dry them, and grind them into powder; add the powder into a 40% v / v ethanol-ammonium sulfate two-phase aqueous system whose weight is 7 times the total weight of the crape myrtle flowers and jasmine flowers, wherein the volume of ethanol to ammonium sulfate is 2:1; perform ultrasonic extraction at 30°C and 350W for 3 hours; collect the extract, concentrate the extract, and spray-dry it to obtain powder A.
[0057] Comparative Example 5
[0058] The difference from Example 1 is that white tea polysaccharide is missing, and the missing raw materials are allocated to other components in proportion. The rest is the same as Example 1.
[0059] The steps of preparing the medicinal tea for nourishing the stomach, aiding digestion and resisting allergy are as follows:
[0060] S1. Wash fresh crape myrtle, jasmine and pomegranate flowers, precool at 3°C for 11h, freeze-dry and grind into powder, add the powder into a 40% v / v ethanol-ammonium sulfate aqueous two-phase system whose weight is 7 times the total weight of crape myrtle, jasmine and pomegranate, wherein the volume of ethanol to ammonium sulfate is 2:1, perform ultrasonic extraction at 30°C and 350W for 3h, collect the extract, concentrate the extract, spray dry and obtain powder A;
[0061] S2, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding an enzyme solution composed of protease, amylase and cellulase in a mass ratio of 1:2:5, which is 5 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam, wherein the enzyme activities of protease, amylase and cellulase are all 90IU / mL, and assisted enzymolysis is carried out under microwave conditions of 500W and 55°C for 2h, and the extract is filtered through an ultrafiltration membrane with a molecular weight cutoff of 3kDa, and the filtrate is concentrated under reduced pressure and spray-dried to obtain powder B;
[0062] S3. Add powder A and powder B into water at a liquid-solid ratio of 15:1 mL / g, stir ultrasonically at 500 rpm and 25°C for 30 min, add natto powder and sodium citrate and continue stirring for 50 min, homogenize at 2500 rpm and 35°C for 8 min to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0063] Comparative Example 6
[0064] The difference from Example 1 is that the ethanol-ammonium sulfate aqueous two-phase system is replaced by ethanol solution, and the rest is the same as Example 1.
[0065] The step S2 of the preparation method of the medicinal tea for nourishing the stomach, aiding digestion and resisting allergy is changed to: wash fresh crape myrtle flowers, jasmine flowers and pomegranate flowers, precool them at 3°C for 11 hours, freeze-dry them, and grind them into powders, add the powders into a 40% v / v ethanol solution whose weight is 7 times the total weight of the crape myrtle flowers, jasmine flowers and pomegranate flowers, perform ultrasonic extraction at 30°C and 350W for 3 hours, collect the extract, concentrate the extract, and spray dry it to obtain powder A;
[0066] Comparative Example 7
[0067] The difference from Example 1 is that all extraction methods in steps S2 and S3 are replaced by conventional water extraction, and the rest are consistent with Example 1.
[0068] The steps of preparing the medicinal tea for nourishing the stomach, aiding digestion and resisting allergy are as follows:
[0069] S1. Grind the white tea into 150 mesh, place it in a supercritical extraction device, set the temperature to 35°C, the pressure to 150 bar, and the CO2 flow rate to 15 mL / min, perform supercritical CO2 extraction for 45 minutes, centrifuge the extract at 4000 rpm for 15 minutes for separation, concentrate the separated liquid under reduced pressure, then use 65% v / v ethanol to precipitate the concentrated liquid for 35 minutes, further purify it, and dry it at 50°C for 3 hours to obtain white tea polysaccharides;
[0070] S2, washing fresh crape myrtle, jasmine and pomegranate flowers, precooling at 3 ° C for 11 hours, freeze-drying, crushing into powder, adding the powder into water, the amount of water added is 7 times the total weight of crape myrtle, jasmine and pomegranate flowers, extracting at 30 ° C for 3 hours, collecting the extract, concentrating the extract, spray drying, and obtaining powder A;
[0071] S3, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding water, the amount of water added is 5 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam, extracting at 55° C. for 2 h, filtering the extract through an ultrafiltration membrane with a molecular weight cutoff of 3 kDa, concentrating the filtrate under reduced pressure, and spray drying to obtain powder B;
[0072] S4. Add powder A and powder B into water at a liquid-solid ratio of 15:1 mL / g, stir ultrasonically at 500 rpm and 25°C for 30 min, add white tea polysaccharide, natto dry powder and sodium citrate and continue stirring for 50 min, homogenize at 2500 rpm and 35°C for 8 min to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
[0073] Test situation
[0074] 1. Test results of stomach-nourishing effect
[0075] 120 healthy SD rats (half male and half female, weighing 150±20g) were taken, and adaptive feeding was performed for one week. The rats were randomly divided into 12 groups, each with 10 rats, recorded as blank control group, model group, Example 1-3 group and Comparative Example 1-7 group. The Example 1-3 group and Comparative Example 1-7 group were given the prepared medicinal tea, twice a day, 10mL each time, and the remaining blank control group and model group were given an equal amount of distilled water for 15 days. The model group, Example 1-3 group and Comparative Example 1-7 group rats were given 200mg / kg acetylsalicylic acid for 4 days. After the last administration, the rats were fasted but not watered for 24 hours, and the animals were killed for sampling. Detection index: observe the size, depth, base cleanliness, presence or absence of white moss, surrounding mucosal color, elasticity and congestion and edema of gastric antral ulcer. The ulcer index was determined and calculated according to Guth. Calculation was based on the length of mucosal ulcers and erosions (mm): spot erosions <1 mm were scored as 1 point, 1-2 mm as 2 points, 2-3 mm as 3 points, 3-4 mm as 4 points, and so on, with 1 point for each additional 1 mm. The score was doubled when the erosion width was >1 mm. The cumulative score of the entire stomach was the ulcer index of the rat. Calculation formula: ulcer inhibition rate (%) = (ulcer index of the model group - ulcer index of the embodiment group or the comparative group) / ulcer index of the model group × 100%. The results are shown in Table 1.
[0076] Table 1
[0077]
[0078] As can be seen from Table 1, compared with Comparative Examples 1-7, the medicinal tea prepared in Examples 1-3 of the present invention can help inhibit gastric ulcers, indicating that it has the effect of nourishing the stomach and aiding digestion.
[0079] 2. Anti-allergy test results
[0080] Experimental method: 120 healthy white guinea pigs (half male and half female, 4-5 weeks old, weighing 150±20g) were randomly divided into 12 groups, 10 in each group, recorded as blank control group, model group, Example 1-3 group and Comparative Example 1-7 group. The prepared medicinal tea was given to Example 1-3 group and Comparative Example 1-7 group respectively, twice a day, 5 mL each time, and the remaining blank control group and model group were given equal amount of distilled water for gavage for 30 days. The white guinea pigs in the model group, Example 1-3 group and Comparative Example 1-7 group were sensitized once on the 1st day and the 8th day, and challenged on the 15th day. The white guinea pigs in the model group, Example 1-3 group and Comparative Example 1-7 group were intradermally injected with 5% trichloroethylene olive oil solution, with a skin induction concentration of 40% and a challenge concentration of 20%. The skin reaction of the animals was observed and recorded 24 hours after the challenge, and then the animals were anesthetized and blood was collected, serum was separated, and serum IgE content was detected. The results are shown in Table 2.
[0081] Table 2
[0082]
[0083] Compared with the blank control group, * P<0.05; compared with the model group, ** P<0.05.
[0084] As can be seen from Table 2, compared with Comparative Examples 1-7, the medicinal tea prepared in Examples 1-3 of the present invention can significantly reduce the content of IgE, indicating that it has a good anti-allergic effect.
[0085] 3. Sensory Evaluation
[0086] 100 people were selected and divided into 10 groups. The stomach-nourishing, digestion-aiding and anti-allergic medicinal teas prepared in Examples 1-3 and Comparative Examples 1-7 of the present invention were tasted and scored from the aspects of taste and smell. The score range was 1-10 points (decimals were allowed). 9-10 points: warm and comfortable taste, full flavor, lasting aftertaste, strong aroma, and fresh and lively tea soup; 7-8 points: smooth taste, moderate taste, moderate aftertaste, moderate aroma, and moderate freshness of tea soup; 4-6 points: uneven taste, weak taste, short aftertaste, weak aroma, and lack of freshness of tea soup; 1-3 points: rough taste, lack of taste, almost no aftertaste, almost no aroma, and stale tea soup. The scores were averaged and the results were recorded as shown in Table 3.
[0087] Table 3
[0088]
[0089] As can be seen from Table 3, compared with Comparative Examples 1-7, the taste and smell of the medicinal tea prepared in Examples 1-3 of the present invention are well received by consumers, indicating that the raw materials, their proportions and process conditions of the present invention are extremely important for the prepared products.
[0090] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A medicinal tea for nourishing the stomach, aiding digestion and resisting allergies, characterized in that: The following raw materials are included in parts by weight: 10-15 parts of crape myrtle flowers, 15-20 parts of jasmine flowers, 10-15 parts of pomegranate flowers, 3-7 parts of white tea polysaccharides, 6-10 parts of coix seeds, 3-5 parts of hericium erinaceus, 1-3 parts of tangerine peel, 2-4 parts of yam, 2-6 parts of natto dry powder, and 1-3 parts of sodium citrate; The preparation steps of the white tea polysaccharide include: crushing white tea, performing supercritical CO2 extraction, separating the extract, concentrating under reduced pressure, precipitating the concentrate with alcohol, refining and drying to obtain the white tea polysaccharide; The preparation method of the medicinal tea comprises the following specific steps: S1. Wash fresh crape myrtle flowers, jasmine flowers and pomegranate flowers, precool, freeze-dry, grind into powder, add into ethanol-ammonium sulfate aqueous two-phase system, perform ultrasonic extraction, collect the extract, concentrate, spray dry, and obtain powder A; S2, crushing coix seeds, hericium erinaceus, tangerine peel and yam, adding them to the enzyme solution, performing microwave-assisted enzymolysis, filtering the extract through an ultrafiltration membrane, concentrating the filtrate under reduced pressure, and spray drying to obtain powder B; The amount of the enzyme solution added is 4-6 times the total weight of coix seeds, hericium erinaceus, tangerine peel and yam; the enzyme solution is composed of protease, amylase and cellulase in a mass ratio of 1:1-3:3-7, and the enzyme activity is 80-100 IU / mL; the microwave-assisted enzymolysis is performed at 400-600W and 50-60°C for 1-3h; S3. Add powder A and powder B into water, stir ultrasonically, add white tea polysaccharide, natto dry powder and sodium citrate, continue stirring, and homogenize to obtain a medicinal tea that nourishes the stomach, aids digestion and has anti-allergic properties.
2. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: In terms of weight, the ingredients include: 13 parts of crape myrtle flowers, 18 parts of jasmine flowers, 13 parts of pomegranate flowers, 5 parts of white tea polysaccharides, 8 parts of coix seeds, 4 parts of hericium erinaceus, 2 parts of tangerine peel, 3 parts of yam, 4 parts of natto dry powder, and 2 parts of sodium citrate.
3. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: The preparation steps of the white tea polysaccharide are specifically as follows: crushing the white tea into 100-200 meshes, performing supercritical CO2 extraction for 30-60 min at a temperature of 30-40° C., a pressure of 100-200 bar, and a flow rate of 10-20 mL / min, separating the extract by centrifugation at 3000-5000 rpm for 10-20 min, concentrating under reduced pressure, precipitating the concentrate with 50-80% v / v ethanol for 30-40 min, refining, and drying at 40-60° C. for 2-4 h to obtain the white tea polysaccharide.
4. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: In step S1, the precooling is performed at 0-5°C for 10-12 hours; and the ultrasonic extraction is performed at a temperature of 20-40°C and a power of 300-400W for 2-4 hours.
5. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: In step S1, the concentration of the ethanol-ammonium sulfate aqueous two-phase system is 30-50% v / v, wherein the volume ratio of ethanol to ammonium sulfate is 1-3:1; and the amount of the ethanol-ammonium sulfate aqueous two-phase system added is 6-8 times the total weight of the crape myrtle flower, jasmine flower and pomegranate flower.
6. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: In step S2, the molecular weight cut-off of the ultrafiltration membrane is 2 kDa-4 kDa.
7. The stomach-nourishing, digestion-aiding and anti-allergic medicinal tea according to claim 1, characterized in that: In step S3, the liquid-to-solid ratio of water to powder A and powder B is 10-20:1 mL / g; the ultrasonic stirring is at 400-600 rpm and 20-30° C. for 20-40 min; the continued stirring time is 40-60 min; the homogenization speed is 2000-3000 rpm, the time is 5-10 min, and the temperature is 30-40° C.
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