A dendrobium beverage for synergistically assisting blood sugar and lipid reduction and preparation method thereof

Abstract

The invention discloses a dendrobium beverage for synergistically assisting hypoglycemic and lipid-lowering and a preparation method thereof. The beverage comprises the following components by weight percentage: 12-18% of dendrobium officinale slurry, 4-8% of kudingcha-walnut green peel composite extraction powder, 2-3% of xylitol, 1-2% of citric acid solution, 0.01-0.02% of composite gel, 0.02-0.03% of high-ester pectin, 0.02-0.03% of sodium hexametaphosphate, and the balance is water. The kudingcha-walnut green peel composite extraction powder is used to enhance the auxiliary hypoglycemic and lipid-lowering effect of the dendrobium beverage, and the composite gel can avoid the occurrence of instability problems such as aggregation, flocculation and precipitation in the system under high or low temperature conditions, thereby improving product quality.

Description

Technical Field

[0001] The invention relates to a beverage containing natural substances and a preparation method thereof, in particular to a dendrobium beverage having synergistic auxiliary blood sugar and lipid lowering functions and a preparation method thereof. Background Art

[0002] Hypertension, hyperlipidemia and hyperglycemia are commonly known as the "three highs". With the improvement of living standards and the acceleration of the pace of life, the "three highs" have become the "number one killer" of human beings. Among them, high blood sugar can cause diabetes, and high blood lipids can cause cardiovascular and cerebrovascular diseases such as vascular embolism. Once suffering from high blood sugar and high blood lipids, patients can only take long-term medication, and medication cannot avoid the problem of high blood sugar and high blood lipids leading to decreased physical function.

[0003] Dendrobium officinale belongs to the genus Dendrobium of the Orchidaceae family and is a precious Chinese medicinal material. Polysaccharides are its main active ingredients. Dendrobium officinale has high medicinal value. Its main functional ingredients include dendrobium polysaccharides, dendrobine, amino acids, peptides, phenolic compounds and some metal elements. Among all the active ingredients, the content of polysaccharides is the highest (18% to 30%). Many studies have shown that Dendrobium has the effects of lowering blood sugar, lowering blood lipids, anti-tumor, anti-oxidation, and improving immunity.

[0004] Traditional Chinese medicine beverages made from natural plants are also gaining popularity, becoming an industry with great market prospects and development potential in my country. Currently, the auxiliary hypoglycemic and lipid-lowering effects of dendrobium beverages on the market mostly rely on polysaccharides extracted from dendrobium, and the auxiliary hypoglycemic and lipid-lowering effects are not obvious. Some compound beverages improve the functional effects of beverages by superimposing raw materials with auxiliary hypoglycemic and lipid-lowering functions. However, in the prior art, the superposition of active ingredients in the raw materials of compound beverages will change the way the active ingredients are absorbed and decomposed in the organism, causing some of the active ingredients in the compound beverage to lose their original functions, and the auxiliary hypoglycemic and lipid-lowering effects are not significantly improved. Now the market urgently needs a dendrobium beverage that has obvious auxiliary hypoglycemic and lipid-lowering effects and is safe and non-toxic. Summary of the invention

[0005] The purpose of the present invention is to provide a stable, safe and non-toxic synergistic auxiliary blood sugar and lipid lowering dendrobium beverage and a preparation method thereof.

[0006] The technical solution of the present invention is a dendrobium beverage for synergistically assisting in reducing blood sugar and lipids. The beverage comprises the following components by weight percentage: 12-18% of dendrobium officinale slurry, 4-8% of kudingcha tea-walnut green peel composite extraction powder, 2-3% of xylitol, 1-2% of citric acid solution, 0.01-0.02% of composite gel, 0.02-0.03% of high-ester pectin, 0.02-0.03% of sodium hexametaphosphate, and the balance is water.

[0007] The preparation method of the above beverage comprises the following steps:

[0008] (1) Preparation of Dendrobium officinale slurry: rub and clean fresh stems of Dendrobium officinale, blanch them in boiling water for 5 minutes, take them out, wash and cut them into small pieces of about 2 cm, blanch them for 2 to 10 minutes, the blanching temperature is 60 to 90° C., and after cooling, add 1 to 4 times the mass of softened water to coarsely crush them; after coarse crushing, grind them at a pressure of 10 to 30 MPa for 5 to 10 minutes to obtain Dendrobium officinale slurry;

[0009] (2) Preparation of Kudingcha-walnut green peel composite extract powder: grind walnut green peel and Kudingcha according to a weight ratio of 1:12-25, add 70% ethanol solution by volume according to the mass concentration of the feed liquid of 0.05-0.1 g / mL for leaching, ultrasonic extraction at a power of 400 W for 30-60 min, let the extract stand for 30 min and centrifuge at a speed of 3500-5000 r / min for 10 min, take the supernatant and filter to obtain Kudingcha-walnut green peel extract, add the composite extractant to the Kudingcha-walnut green peel extract, mix evenly, and the composite extractant occupies 80% of the total volume of the mixed solution. 60% of the total volume, heated in a water bath at 20-30°C for 10 minutes, and centrifuged to obtain centrifuged liquid 1 and solid matter 1; continued to add 10% by volume of the composite extractant to the centrifuged liquid 1, heated in a water bath at 40-50°C for 10 minutes, and centrifuged again to obtain centrifuged liquid 2 and solid matter 2; continued to add the composite extractant to the centrifuged liquid 2 until the volume fraction of the composite extractant was 90%, heated in a water bath at 60-70°C for 10 minutes, and centrifuged to obtain centrifuged liquid 3 and solid matter 3; the solid matter obtained by the three centrifugations was washed and dried to obtain the Kudingcha-walnut green peel composite extraction powder;

[0010] (3) Preparation of Dendrobium beverage: First add Kudingcha tea-walnut green peel composite extract powder to the Dendrobium officinale slurry, then add xylitol, citric acid solution, composite gel, high ester pectin, sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool.

[0011] Furthermore, the preparation method of the composite extractant is:

[0012] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 4 to 6 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 55 to 70°C for 3 hours, 24 to 58% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 40 to 60°C for 12 hours, and then filtered, the filtrate is concentrated to 40 to 50% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0013] Furthermore, the preparation method of the composite gel is:

[0014] 1) Peach gum is crushed and passed through a 50-mesh sieve to obtain peach gum powder, water is added according to a solid-liquid ratio of 0.3-0.9 g / L, and after swelling for 24-48 hours, extraction is performed at 90° C. with stirring for 1-3 hours, and the supernatant is obtained by centrifugation after cooling;

[0015] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.0-1.5 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.5-10, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1-2.5 hours to obtain solution 1;

[0016] 3) Add 8 to 15 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 20 to 46 parts of the supernatant obtained in step 1), stir evenly, heat to 65 to 80° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0017] Beneficial effects:

[0018] (1) Flavonoids have antioxidant activity and hypoglycemic function. Kuding tea contains rich flavonoid active substances, which have hypoglycemic effect after being compounded with Dendrobium candidum. Kuding tea contains natural polyphenols, which also have obvious hypoglycemic effect. However, Kuding tea combined phenol is easily hydrolyzed in the gastric environment, resulting in Kuding tea-containing beverages mainly relying on flavonoids extracted from Kuding tea to exert hypoglycemic effect, and the hypoglycemic effect of Kuding tea cannot be fully exerted. The scheme of the present invention forms a conjugate by walnut green peel extract and Kuding tea combined phenol, thereby reducing the digestibility of Kuding tea combined phenol, which is beneficial to the body's digestion and absorption of hypoglycemic active ingredients, and further improves the auxiliary hypoglycemic effect of the beverage.

[0019] (2) Unsaturated fatty acids have a lipid-lowering effect. The unsaturated fatty acids contained in purslane can effectively inhibit pancreatic lipase and have a good lipid-lowering effect. This process can promote the phenolic substances in purslane to activate the body's thermogenic fat and induce the transformation of energy storage fat cells into thermogenic fat precursors, inhibiting the expression of energy storage fat-specific genes, thereby regulating energy metabolism balance and further promoting lipid metabolism.

[0020] (3) The effects of different extraction solvents on the hypoglycemic properties of flavonoids and combined phenols contained in Kudingcha are significantly different. As the polarity of the solvent decreases, the content of active substances and the hypoglycemic properties of Kudingcha also decrease. The scheme of the present invention combines the phytosterols contained in sunflower seeds with the unsaturated fatty acids in purslane to obtain a variety of unsaturated fatty acid phytosterol esters, so that the composite extractant can efficiently extract the active substances in Kudingcha without destroying the hypoglycemic properties of the active substances.

[0021] (4) Due to the large difference in properties between the raw material slurry and the particles of the auxiliary blood sugar and lipid lowering active ingredient, there are obvious differences in particle migration and particle size changes at different temperatures. The use of conventional stabilizers will also cause the system to aggregate, flocculate and precipitate under high or low temperature conditions, affecting product quality. Modification methods such as degradation, oxidation, carboxyl alkylation, hydroxyalkylation and hydrophobization of active groups such as hydroxyl groups and glycosidic bonds in the agar molecular structure can give it more and stronger functional properties. The scheme of the present invention uses agar activated by 2,3-epoxypropyl to react with highly branched sugar groups in peach gum to form a composite gel with an interconnected void structure, thereby improving the suspension force of the gel molecules in the system, controlling the particle migration rate in the system, and improving the stability of the system.

[0022] (5) The peach gum polysaccharide invention scheme utilizes peach gum polysaccharide to form a composite gel to improve the stability of the system. On the other hand, since peach gum has many functional groups, good water solubility, good biocompatibility and good emulsification, it can well compensate for the defects of the raw material slurry and the auxiliary blood sugar and lipid lowering active ingredient particles in the invention scheme that the properties are quite different, and the particles agglomerate under high or low temperature conditions, resulting in system instability. DETAILED DESCRIPTION

[0023] Example 1

[0024] Preparation of composite extractant:

[0025] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 4 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 70°C for 3 hours, 24% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the pH of the solution to neutral, the mixture is heated in a water bath at 60°C for 12 hours, and then filtered, the filtrate is concentrated to 40% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0026] Preparation of composite gel:

[0027] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.3 g / L, and after swelling for 24 h, the powder was extracted by stirring at 90° C. for 1 h, and the supernatant was obtained by centrifugation after cooling;

[0028] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.0 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 10, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1 hour to obtain solution 1;

[0029] 3) Add 15 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 20 parts of the supernatant obtained in step 1), stir evenly, heat to 80° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0030] Preparation of Dendrobium drink for synergistically assisting in reducing blood sugar and lipids:

[0031] (1) Preparation of Dendrobium officinale slurry: fresh stems of Dendrobium officinale were scrubbed clean, blanched in boiling water for 5 minutes, taken out, washed, cut into small pieces of about 2 cm, blanched for 2 minutes at 60° C., cooled, and then coarsely crushed by adding 1 times the mass of softened water; after coarsely crushing, the pulping treatment was performed at a pressure of 10 MPa for 5 minutes to obtain Dendrobium officinale slurry;

[0032] (2) Preparation of Kudingcha-walnut green peel composite extract powder: The ground walnut green peel and Kudingcha were mixed in a weight ratio of 1:12, and an ethanol solution with a volume fraction of 70% was added to the material solution at a mass concentration of 0.1 g / mL for leaching, and ultrasonic extraction was performed at a power of 400 W for 60 minutes. The extract was allowed to stand for 30 minutes and then centrifuged at a speed of 3500 r / min for 10 minutes. The supernatant was filtered to obtain a Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract and mixed evenly, with the composite extractant accounting for 60% of the total volume of the mixed solution. %, heating in a water bath at 30°C for 10 minutes, centrifuging to obtain centrifuge liquid 1 and solid matter 1; continuing to add 10% by volume of the composite extractant to the centrifuge liquid 1, heating in a water bath at 50°C for 10 minutes, and centrifuging again to obtain centrifuge liquid 2 and solid matter 2; continuing to add the composite extractant to the centrifuge liquid 2 until the volume fraction of the composite extractant is 90%, heating in a water bath at 60°C for 10 minutes, and centrifuging to obtain centrifuge liquid 3 and solid matter 3; washing and drying the solid matter obtained by the three centrifugations to obtain Kudingcha-walnut green peel composite extraction powder;

[0033] (3) Preparation of Dendrobium beverage: add 12% of Dendrobium officinale slurry and 8% of Kudingcha tea-walnut green peel composite extract powder by weight percentage, then add 2% of xylitol, 2% of citric acid solution, 0.01% of composite gel, 0.03% of high ester pectin, and 0.02% of sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool.

[0034] Example 2

[0035] Preparation of composite extractant:

[0036] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 6 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 55°C for 3 hours, 58% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the pH of the solution to neutral, the mixture is heated in a water bath at 40°C for 12 hours, and then filtered, the filtrate is concentrated to 50% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0037] Preparation of composite gel:

[0038] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.9 g / L, and after swelling for 48 h, the powder was extracted by stirring at 90° C. for 3 h, and the supernatant was obtained by centrifugation after cooling;

[0039] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.5 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.5, mixing the solution, and reacting in a constant temperature water bath at 50° C. for 2.5 h to obtain solution 1;

[0040] 3) Add 8 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 46 parts of the supernatant obtained in step 1), stir evenly, heat to 65° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0041] Preparation of Dendrobium drink for synergistically assisting in reducing blood sugar and lipids:

[0042] (1) Preparation of Dendrobium officinale slurry: fresh stems of Dendrobium officinale were scrubbed clean, blanched in boiling water for 5 minutes, taken out, washed, cut into small pieces of about 2 cm, blanched for 10 minutes at 90° C., cooled, and then coarsely crushed by adding 4 times the mass of softened water; after coarse crushing, the pulping treatment was performed at a pressure of 30 MPa for 10 minutes to obtain Dendrobium officinale slurry;

[0043] (2) Preparation of Kudingcha-walnut green peel composite extract powder: The ground walnut green peel and Kudingcha were mixed at a weight ratio of 1:25, and an ethanol solution with a volume fraction of 70% was added to the mixture at a mass concentration of 0.05 g / mL for extraction. The mixture was subjected to ultrasonic extraction at a power of 400 W for 30 min. The extract was allowed to stand for 30 min and then centrifuged at a speed of 5000 r / min for 10 min. The supernatant was filtered to obtain a Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract and the mixture was mixed evenly. The composite extractant accounted for 60% of the total volume of the mixture. %, heating in a water bath at 30°C for 20 minutes, centrifuging to obtain centrifuge liquid 1 and solid matter 1; continuing to add 10% by volume of the composite extractant to the centrifuge liquid 1, heating in a water bath at 40°C for 10 minutes, and centrifuging again to obtain centrifuge liquid 2 and solid matter 2; continuing to add the composite extractant to the centrifuge liquid 2 until the volume fraction of the composite extractant is 90%, heating in a water bath at 70°C for 10 minutes, and centrifuging to obtain centrifuge liquid 3 and solid matter 3; washing and drying the solid matter obtained by the three centrifugations to obtain Kudingcha-walnut green peel composite extraction powder;

[0044] (3) Preparation of Dendrobium beverage: add 18% of Dendrobium officinale slurry and 4% of Kudingcha tea-walnut green peel composite extract powder by weight percentage, then add 3% of xylitol, 1% of citric acid solution, 0.02% of composite gel, 0.02% of high ester pectin, and 0.03% of sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool.

[0045] Example 3

[0046] Preparation of composite extractant:

[0047] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 63°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 50°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0048] Preparation of composite gel:

[0049] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.6 g / L, and after swelling for 36 hours, the powder was extracted by stirring at 90° C. for 2 hours, and the supernatant was obtained by centrifugation after cooling;

[0050] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.3 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.8, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1.8 h to obtain solution 1;

[0051] 3) Add 11.5 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 33 parts of the supernatant obtained in step 1), stir evenly, heat to 73° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0052] Preparation of Dendrobium drink for synergistically assisting in reducing blood sugar and lipids:

[0053] (1) Preparation of Dendrobium officinale slurry: fresh stems of Dendrobium officinale were scrubbed clean, blanched in boiling water for 5 minutes, taken out, washed, cut into small pieces of about 2 cm, blanched for 6 minutes at a blanching temperature of 75° C., cooled, and then coarsely crushed by adding 2.5 times the mass of softened water; after coarsely crushing, the pulping treatment was performed at a pressure of 20 MPa for 8 minutes to obtain Dendrobium officinale slurry;

[0054] (2) Preparation of Kudingcha-walnut green peel composite extract powder: The ground walnut green peel and Kudingcha were mixed at a weight ratio of 1:19, and an ethanol solution with a volume fraction of 70% was added to the mixture at a mass concentration of 0.08 g / mL for extraction. The mixture was subjected to ultrasonic extraction at a power of 400 W for 45 min. The extract was allowed to stand for 30 min and then centrifuged at a speed of 4250 r / min for 10 min. The supernatant was filtered to obtain a Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract and the mixture was mixed evenly. The composite extractant accounted for 60% of the total volume of the mixture. %, heating in a water bath at 25°C for 20 minutes, centrifuging to obtain centrifuge liquid 1 and solid matter 1; continuing to add 10% by volume of the composite extractant to the centrifuge liquid 1, heating in a water bath at 45°C for 10 minutes, and centrifuging again to obtain centrifuge liquid 2 and solid matter 2; continuing to add the composite extractant to the centrifuge liquid 2 until the volume fraction of the composite extractant is 90%, heating in a water bath at 65°C for 10 minutes, and centrifuging to obtain centrifuge liquid 3 and solid matter 3; washing and drying the solid matter obtained by the three centrifugations to obtain Kudingcha-walnut green peel composite extraction powder;

[0055] (3) Preparation of Dendrobium beverage: add 15% of Dendrobium officinale slurry and 6% of Kudingcha tea-walnut green peel composite extract powder by weight percentage, then add 2.5% of xylitol, 1.5% of citric acid solution, 0.01% of composite gel, 0.03% of high ester pectin, and 0.03% of sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool.

[0056] Comparative Example 1

[0057] The difference of this comparative example is that in the process of preparing the synergistic auxiliary blood sugar and lipid lowering dendrobium beverage, kudingcha powder is used to replace kudingcha-walnut green peel composite extraction powder;

[0058] Preparation of Kudingcha powder: The ground Kudingcha is added to a 70% ethanol solution with a mass concentration of 0.08 g / mL for extraction, and ultrasonic extraction is performed at a power of 400 W for 45 minutes. The extract is centrifuged for 30 minutes at a centrifugal speed of 4250 r / min and a centrifugal time of 10 minutes. The supernatant is filtered to obtain a Kudingcha extract. A composite extractant is added to the Kudingcha extract, and the mixture is mixed evenly, wherein the composite extractant accounts for 60% of the total volume of the mixture. The mixture is heated in a water bath at 25° C. for 20 minutes. Centrifugal separation is performed to obtain centrifugal liquid 1 and solid matter 1; 10% by volume of the composite extractant is continuously added to the centrifugal liquid 1, heated in a water bath at 45°C for 10 minutes, and centrifuged again to obtain centrifugal liquid 2 and solid matter 2; the composite extractant is continuously added to the centrifugal liquid 2 until the volume fraction of the composite extractant is 90%, heated in a water bath at 65°C for 10 minutes, and centrifuged to obtain centrifugal liquid 3 and solid matter 3; the solid matter obtained by the three centrifugal separations is washed and dried to obtain Kudingcha powder.

[0059] The rest is the same as in Example 3.

[0060] Comparative Example 2

[0061] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, tangerine peel rich in flavonoids is used instead of kudingcha to prepare tangerine peel-walnut green peel composite extraction powder;

[0062] Preparation of tangerine peel-walnut green peel composite extract powder: the ground walnut green peel and tangerine peel are mixed in a weight ratio of 1:19, and a 70% ethanol solution is added to the slurry according to a mass concentration of 0.08 g / mL for leaching, and ultrasonic extraction is performed at a power of 400 W for 45 minutes. The extract is allowed to stand for 30 minutes and centrifuged at a speed of 4250 r / min for 10 minutes. The supernatant is filtered to obtain a tangerine peel-walnut green peel extract, and a composite extractant is added to the tangerine peel-walnut green peel extract, and the composite extractant accounts for 60% of the total volume of the mixed solution. The method comprises the following steps: heating in a water bath at 5°C for 20 minutes, and centrifuging to obtain a centrifuge liquid 1 and a solid substance 1; continuously adding a composite extractant with a volume fraction of 10% to the centrifuge liquid 1, heating in a water bath at 45°C for 10 minutes, and centrifuging again to obtain a centrifuge liquid 2 and a solid substance 2; continuously adding the composite extractant to the centrifuge liquid 2 until the volume fraction of the composite extractant is 90%, heating in a water bath at 65°C for 10 minutes, and centrifuging to obtain a centrifuge liquid 3 and a solid substance 3; and washing and drying the solid substances obtained by the three centrifugations to obtain a tangerine peel-walnut green peel composite extraction powder.

[0063] The rest is the same as in Example 3.

[0064] Comparative Example 3

[0065] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, the preparation of the Kudingcha-walnut green peel composite extract powder is as follows:

[0066] The ground walnut green peel and Kudingcha were mixed in a weight ratio of 1:19, and an ethanol solution with a volume fraction of 70% was added according to a feed liquid mass concentration of 0.08 g / mL for leaching. The ultrasonic extraction was carried out at a power of 400 W for 45 minutes. The extract was allowed to stand for 30 minutes and centrifuged at a speed of 4250 r / min for 10 minutes. The supernatant was filtered to obtain a Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract until the volume fraction of the composite extractant was 90%. The mixture was mixed evenly, with the composite extractant accounting for 60% of the total volume of the mixed solution. The mixture was heated in a water bath at 45°C for 40 minutes. The solid matter obtained by centrifugation was washed and dried to obtain a Kudingcha-walnut green peel composite extract powder.

[0067] The rest is the same as in Example 3.

[0068] Comparative Example 4

[0069] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, the preparation of the Kudingcha-walnut green peel composite extract powder is as follows:

[0070] The ground walnut green peel and Kudingcha were mixed in a weight ratio of 1:19, and an ethanol solution with a volume fraction of 70% was added according to a feed liquid mass concentration of 0.08g / mL for leaching. The ultrasonic extraction was performed at a power of 400W for 45min, and the extract was centrifuged for 30min at a centrifugal speed of 4250r / min and a centrifugal time of 10min. The supernatant was filtered to obtain Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract, and the composite extractant accounted for 30% of the total volume of the mixed solution, and the mixture was heated in a water bath at 25°C for 20min. Centrifugal separation is performed to obtain centrifugal liquid 1 and solid matter 1; the composite extractant is continuously added to centrifugal liquid 2 until the volume fraction of the composite extractant is 60%, the mixture is heated in a water bath at 45°C for 10 minutes, and centrifugal separation is performed again to obtain centrifugal liquid 2 and solid matter 2; the composite extractant is continuously added to centrifugal liquid 2 until the volume fraction of the composite extractant is 90%, the mixture is heated in a water bath at 65°C for 10 minutes, and centrifugal separation is performed to obtain centrifugal liquid 3 and solid matter 3; the solid matter obtained by the three centrifugal separations is washed and dried to obtain Kudingcha tea-walnut green peel composite extraction powder.

[0071] The rest is the same as in Example 3.

[0072] Comparative Example 5

[0073] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, the preparation of the Kudingcha-walnut green peel composite extract powder is as follows:

[0074] The ground walnut green peel and Kudingcha were mixed in a weight ratio of 1:19, and an ethanol solution with a volume fraction of 70% was added according to a feed liquid mass concentration of 0.08 g / mL for leaching. The ultrasonic extraction was performed at a power of 400 W for 45 min, and the extract was centrifuged for 30 min at a centrifugal speed of 4250 r / min and a centrifugal time of 10 min. The supernatant was filtered to obtain Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract, and the composite extractant accounted for 60% of the total volume of the mixed solution. The mixture was heated in a water bath at 25°C for 20 min, centrifugation to obtain centrifugal liquid 1 and solid matter 1; continue to add 10% volume fraction of the composite extractant to the centrifugal liquid 1, heat in a water bath at 25°C for 10 minutes, and centrifuge again to obtain centrifugal liquid 2 and solid matter 2; continue to add the composite extractant to the centrifugal liquid 2 until the volume fraction of the composite extractant is 90%, heat in a water bath at 25°C for 10 minutes, and centrifuge to obtain centrifugal liquid 3 and solid matter 3; wash and dry the solid matter obtained by the three centrifugations to obtain Kudingcha-walnut green peel composite extraction powder.

[0075] The rest is the same as in Example 3.

[0076] Comparative Example 6

[0077] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, the preparation of the Kudingcha-walnut green peel composite extract powder is as follows:

[0078] The ground walnut green peel and Kudingcha were mixed in a weight ratio of 1:19, and an ethanol solution with a volume fraction of 70% was added according to a feed liquid mass concentration of 0.08 g / mL for leaching. The ultrasonic extraction was performed at a power of 400 W for 45 min, and the extract was centrifuged for 30 min at a centrifugal speed of 4250 r / min and a centrifugal time of 10 min. The supernatant was filtered to obtain Kudingcha-walnut green peel extract. A composite extractant was added to the Kudingcha-walnut green peel extract, and the composite extractant accounted for 60% of the total volume of the mixed solution. The mixture was heated in a water bath at 65°C for 20 min, centrifugation to obtain centrifugal liquid 1 and solid matter 1; continue to add 10% volume fraction of the composite extractant to the centrifugal liquid 1, heat in a water bath at 65°C for 10 minutes, and centrifuge again to obtain centrifugal liquid 2 and solid matter 2; continue to add the composite extractant to the centrifugal liquid 2 until the volume fraction of the composite extractant is 90%, heat in a water bath at 65°C for 10 minutes, and centrifuge to obtain centrifugal liquid 3 and solid matter 3; wash and dry the solid matter obtained by the three centrifugations to obtain Kudingcha-walnut green peel composite extraction powder.

[0079] The rest is the same as in Example 3.

[0080] Comparative Example 7

[0081] The difference of this comparative example is that in the process of preparing the synergistic auxiliary hypoglycemic and lipid-lowering dendrobium beverage, 75% by volume ethanol is used to replace the composite extractant to prepare the Kudingcha-walnut green peel composite extraction powder;

[0082] Preparation of Kudingcha-walnut green peel composite extract powder: The ground walnut green peel and Kudingcha are mixed in a weight ratio of 1:19, and a 70% ethanol solution is added to the material liquid at a mass concentration of 0.08 g / mL for extraction. The ultrasonic extraction is performed at a power of 400 W for 45 minutes. The extract is allowed to stand for 30 minutes and centrifuged at a speed of 4250 r / min for 10 minutes. The supernatant is filtered to obtain a Kudingcha-walnut green peel extract. 75% ethanol by volume is added to the Kudingcha-walnut green peel extract as an extractant, and the mixture is mixed evenly. The extractant accounts for 1% of the mixed solution. The method comprises the following steps: adding 10% of the total volume of the extractant to the centrifugal liquid 1, heating the extractant in a water bath at 45°C for 10 minutes, and centrifuging to obtain a centrifugal liquid 2 and a solid substance 2; adding the extractant to the centrifugal liquid 2 until the volume fraction of the extractant is 90%, heating the extractant in a water bath at 65°C for 10 minutes, and centrifuging to obtain a centrifugal liquid 3 and a solid substance 3; washing and drying the solid substances obtained by the three centrifugal separations to obtain a Kudingcha tea-walnut green peel composite extraction powder.

[0083] The rest is the same as in Example 3.

[0084] Comparative Example 8

[0085] This comparative example prepares a composite extractant:

[0086] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 50°C for 3 hours. Purslane in an amount of 41% by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 50°C for 12 hours, and then filtered. After the filtrate is concentrated to 45% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0087] The rest is the same as in Example 3.

[0088] Comparative Example 9

[0089] This comparative example prepares a composite extractant:

[0090] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 75°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 50°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0091] The rest is the same as in Example 3.

[0092] Comparative Example 10

[0093] This comparative example prepares a composite extractant:

[0094] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, and the amount of sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, heated in a water bath at 63°C for 3 hours, acetic acid solution is added to adjust the pH of the solution to neutral, heated in a water bath at 50°C for 12 hours and filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, and an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0095] The rest is the same as in Example 3.

[0096] Comparative Example 11

[0097] This comparative example prepares a composite extractant:

[0098] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 63°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 35°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0099] The rest is the same as in Example 3.

[0100] Comparative Example 12

[0101] This comparative example prepares a composite extractant:

[0102] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 63°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 65°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, an equal volume of glycidyl ester is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0103] The rest is the same as in Example 3.

[0104] Comparative Example 13

[0105] This comparative example prepares a composite extractant:

[0106] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 63°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 50°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, and an equal volume of glycerol is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0107] The rest is the same as in Example 3.

[0108] Comparative Example 14

[0109] This comparative example prepares a composite extractant:

[0110] The roasted sunflower seeds are crushed and passed through a 400-mesh sieve to obtain sunflower seed powder, 0.8 mol / L sodium bicarbonate solution is added, the amount of the sodium bicarbonate solution added is 5 times the weight of the sunflower seed powder, and the mixture is heated in a water bath at 63°C for 3 hours, 41% of purslane by weight of the sunflower seed powder is added, acetic acid solution is added to adjust the solution pH to neutral, the mixture is heated in a water bath at 50°C for 12 hours, and then filtered, the filtrate is concentrated to 45% of the volume in a rotary evaporator, and an equal volume of ethyl acetate is added for extraction 3 times, and the extract is collected to obtain a composite extractant.

[0111] The rest is the same as in Example 3.

[0112] Comparative Example 15

[0113] The difference of this comparative example is that no composite gel is added during the preparation of the synergistic auxiliary blood sugar and lipid lowering dendrobium beverage;

[0114] Preparation of dendrobium beverage: add 15% of dendrobium officinale slurry and 6% of kudingcha tea-walnut green peel composite extract powder by weight percentage, then add 2.5% of xylitol, 1.5% of citric acid solution, 0.03% of high-ester pectin, and 0.03% of sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool.

[0115] Comparative Example 16

[0116] This comparative example prepares the composite gel:

[0117] 1) guar gum flakes were crushed through a 50-mesh sieve to obtain guar gum powder, water was added according to a solid-liquid ratio of 0.6 g / L, and the mixture was swollen for 36 h, and then stirred and extracted at 90° C. for 2 h. After cooling, the mixture was centrifuged to obtain a supernatant;

[0118] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.3 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.8, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1.8 h to obtain solution 1;

[0119] 3) Add 11.5 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 33 parts of the supernatant obtained in step 1), stir evenly, heat to 73° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0120] The rest is the same as in Example 3.

[0121] Comparative Example 17

[0122] This comparative example prepares the composite gel:

[0123] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.6 g / L, and after swelling for 36 hours, the powder was extracted by stirring at 90° C. for 2 hours, and the supernatant was obtained by centrifugation after cooling;

[0124] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.3 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.8, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1.8 h to obtain solution 1;

[0125] 3) Add 33 parts of the supernatant obtained in step 1) to 100 parts of solution 1, stir evenly, raise the temperature to 73° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0126] The rest is the same as in Example 3.

[0127] Comparative Example 18

[0128] This comparative example prepares the composite gel:

[0129] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.6 g / L, and after swelling for 36 hours, the powder was extracted by stirring at 90° C. for 2 hours, and the supernatant was obtained by centrifugation after cooling;

[0130] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.3 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.8, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1.8 h to obtain solution 1;

[0131] 3) Add 11.5 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 33 parts of the supernatant obtained in step 1), stir evenly, heat to 60° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0132] The rest is the same as in Example 3.

[0133] Comparative Example 19

[0134] This comparative example prepares the composite gel:

[0135] 1) Peach gum was crushed and passed through a 50-mesh sieve to obtain peach gum powder, water was added according to a solid-liquid ratio of 0.6 g / L, and after swelling for 36 hours, the powder was extracted by stirring at 90° C. for 2 hours, and the supernatant was obtained by centrifugation after cooling;

[0136] 2) adding 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.3 g / L, stirring and dispersing, adding sodium bicarbonate solution to adjust the pH to 9.8, mixing the solution, and reacting it in a constant temperature water bath at 50° C. for 1.8 h to obtain solution 1;

[0137] 3) Add 11.5 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 33 parts of the supernatant obtained in step 1), stir evenly, heat to 85° C., react at constant temperature for 3 hours, filter the product, wash it twice with 40% ethanol by volume, and dry the product to obtain a composite gel.

[0138] Performance Testing

[0139] Auxiliary hypoglycemic and lipid-lowering performance: 5-week-old male C57BL / 6 mice were purchased and randomly divided into groups with 10 mice in each group. All animals were rested for 1 week before the experiment. The dendrobium beverage prepared in the embodiment or comparative example was gavaged at a dose of 0.1 ml / 10 g·BW, and the blank control group was given an equal amount of normal saline. Each group was gavaged once a day for 20 days. After 20 days of gavage, the growth and weight of the mice were recorded, and blood was collected to detect biochemical indicators. The main examination indicators include triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein and blood sugar.

[0140] TC, total cholesterol; TG, triglycerides; HDL-C, high-density lipoprotein; LDL-C, low-density lipoprotein; GLU, blood glucose

[0141] Stability performance: After the finished beverage is stored at a temperature of T℃ for 48 hours, it is analyzed by vertical scanning macro analysis (Turbiscan). The sample to be tested is placed in the sample pool, and 840nm near-infrared light is used as the light source to scan the sample. The scanning temperature is T℃, the total scanning time is 60min, and the scanning interval time is 5min. According to the principle of multiple light scattering, when the average diameter of particles in the liquid system changes in the dispersed phase of the system, the intensity of transmitted light and the intensity of backscattered light will change accordingly. The stability of the system is quantified by the instability kinetic index (TSI). If the TSI value is larger, it means that the particles in the system move violently and the stability of the system is worse. On the contrary, the smaller the TSI value is, the better the stability of the system.

[0142] The technical indicators of Examples 1 to 3 of the present invention are compared with those of Comparative Examples 1 to 19, as shown in Tables 1 to 3.

[0143] Table 1

[0144] <![CDATA[GLU(mmol·L -1 )]]> Blank control group 23.07±2.21 Example 1 15.36±1.01 Example 2 16.12±1.15 Example 3 15.06±0.93 Comparative Example 1 20.34±1.74 Comparative Example 2 20.74±0.98 Comparative Example 3 19.36±2.74 Comparative Example 4 17.36±1.48 Comparative Example 5 18.02±1.17 Comparative Example 6 17.95±1.23

[0145] Table 2

[0146]

[0147] Table 3

[0148]

[0149] Combining the blank control group, Examples 1 to 3 and Comparative Examples 1 to 6 in Table 1, it can be seen that the present invention combines the peach green peel extract with Kudingcha, and adopts a specific extractant and extraction method in the process, which significantly improves the body's digestion and absorption of hypoglycemic active ingredients, and has a significant hypoglycemic effect; Combining the blank control group, Examples 1 to 3 and Comparative Examples 7 to 14 in Table 2, it can be seen that the phytosterols contained in sunflower seeds are combined with the unsaturated fatty acids in Portulaca oleracea, and the obtained composite extractant can efficiently extract the active substances in Kudingcha, and achieve the lipid-lowering function without destroying the hypoglycemic active substances; Combining Examples 1 to 3 and Comparative Examples 15 to 19 in Table 3, it can be seen that the composite gel formed by peach gum polysaccharide can improve the stability of the system, making up for the fact that the properties of the Dendrobium officinale slurry and the auxiliary hypoglycemic and lipid-lowering active ingredient particles in the invention scheme are quite different, and the particles agglomerate under high or low temperature conditions, resulting in system instability.

Claims

1. A dendrobium beverage that assists in reducing blood sugar and lipids, characterized in that: The beverage comprises the following components by weight percentage: 12-18% of Dendrobium officinale slurry, 4-8% of Kudingcha tea-walnut green peel composite extraction powder, 2-3% of xylitol, 1-2% of citric acid solution, 0.01-0.02% of composite gel, 0.02-0.03% of high ester pectin, 0.02-0.03% of sodium hexametaphosphate, and the balance is water; The preparation method of the beverage comprises the following steps: (1) preparing a Dendrobium officinale slurry: scrubbing and cleaning fresh stems of Dendrobium officinale, blanching them in boiling water for 5 minutes, taking them out, washing and cutting them into 2 cm small segments, blanching them for 2 to 10 minutes at a blanching temperature of 60 to 90° C., cooling them with water, adding 1 to 4 times the mass of softened water for coarse crushing; after coarse crushing, grinding them at a pressure of 10 to 30 MPa for 5 to 10 minutes to obtain a Dendrobium officinale slurry; (2) Preparation of Kudingcha-walnut green peel composite extract powder: grind walnut green peel and Kudingcha in a weight ratio of 1:12-25, add 70% ethanol solution by volume at a feed liquid mass concentration of 0.05-0.1 g / mL for leaching, ultrasonic extraction at a power of 400 W for 30-60 min, let the extract stand for 30 min and centrifuge at a speed of 3500-5000 r / min for 10 min, take the supernatant and filter to obtain Kudingcha-walnut green peel extract, add composite extractant to Kudingcha-walnut green peel extract, mix evenly, the composite extractant accounts for 60% of the total volume of the mixed solution, heat in a water bath at 20-30° C. for 10 min, and centrifuge to obtain centrifuge liquid 1 and solid matter 1; Adding 10% by volume of the composite extractant to the centrifugal separation liquid 1, heating in a water bath at 40-50° C. for 10 min, and centrifuging again to obtain the centrifugal separation liquid 2 and the solid matter 2; Continue to add the composite extractant to the centrifugal separation liquid 2 until the volume fraction of the composite extractant is 90%, heat in a water bath at 60-70° C. for 10 min, and centrifuge to obtain the centrifugal separation liquid 3 and the solid matter 3; The solid matter obtained by three centrifugal separations is washed and dried to obtain Kudingcha tea-walnut green peel composite extraction powder; (3) Preparation of Dendrobium beverage: first add Kudingcha-walnut peel composite extract powder to the Dendrobium officinale slurry, then add xylitol, citric acid solution, composite gel, high ester pectin, sodium hexametaphosphate in sequence, add water and mix again, then homogenize, fill, sterilize and cool; The preparation method of the composite extractant comprises the following steps: crushing roasted sunflower seeds and passing them through a 400-mesh sieve to obtain sunflower seed powder, adding a 0.8 mol / L sodium bicarbonate solution, wherein the amount of the sodium bicarbonate solution added is 4 to 6 times the weight of the sunflower seed powder, heating in a water bath at 55 to 70° C. for 3 hours, adding 24 to 58% of purslane by weight of the sunflower seed powder, adding an acetic acid solution to adjust the pH of the solution to neutral, heating in a water bath at 40 to 60° C. for 12 hours, filtering, concentrating the filtrate to 40 to 50% of the volume in a rotary evaporator, adding an equal volume of glycidyl ester for extraction 3 times, and collecting the extract to obtain the composite extractant; The preparation method of the composite gel comprises the following steps: 1) crushing peach gum through a 50-mesh sieve to obtain peach gum powder, adding water according to a solid-liquid ratio of 0.3-0.9 g / L, swelling for 24-48 hours, extracting with stirring at 90° C. for 1-3 hours, cooling and centrifuging to obtain a supernatant; 2) Add 80% ethanol solution by volume to agar at a solid-liquid ratio of 1.0-1.5 g / L, stir and disperse, then add sodium bicarbonate solution to adjust the pH to 9.5-10, mix the solution, and react in a constant temperature water bath at 50°C for 1-2.5 hours to obtain solution 1; 3) Add 8-15 parts of 2,3-epoxypropyl ester to 100 parts of solution 1, add 20-46 parts of the supernatant obtained in step 1), stir evenly, raise the temperature to 65-80° C., react at a constant temperature for 3 hours, filter the product, wash it twice with 40% by volume ethanol, and dry the product to obtain a composite gel.

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