A Method for Accelerating Generation and LED Light Regulation in Rapid Propagation of Soybeans

Abstract

The present invention discloses a method for regulating soybean rapid multiplication and generation addition by LED light, which includes: after soybean seeds germinate and enter the cotyledon stage, regulating the light quality, light intensity and photoperiod based on the growth stage of soybean until the soybean is completely mature; the growth stages of soybean include the cotyledon stage, the stage from the cotyledon stage to the second branching stage, the stage from the second branching stage to the flower and pod stage, the stage from the flower and pod stage to the maturity stage, and the maturity stage; the pods harvested after the soybean matures are dried and then stored at low temperature. The present invention provides an LED light regulation method and a cultivation method for significantly shortening the breeding generation propagation time of dryland soybeans based on the dynamic LED signal spectrum. Compared with the traditional simple photoperiod and light intensity regulation methods, while significantly shortening the breeding generation propagation time, it can ensure the germination rate of seeds during the rapid propagation process, providing an effective technical approach and method for ensuring sufficient high-quality offspring samples required for the purification process of rapid breeding generations of crops in a controllable environment.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural biotechnology, and particularly relates to a method for regulating LED light for rapid multiplication and generation acceleration of soybeans. Background Art

[0002] The multiplication and generation acceleration of high-quality soybean varieties is a key link in the breeding of new soybean varieties. In the process of soybean cross-breeding, it takes at least 10 generations from cross combination to variety approval. Among them, the first 5-6 generations are mainly for self-purification of heterozygous materials. The whole breeding process is very time-consuming and laborious, seriously restricting the speed of soybean trait improvement. Therefore, shortening the breeding cycle by accelerating the self-breeding speed of early generations has always been the goal pursued by soybean breeders.

[0003] At present, the main method for accelerating generations in conventional soybean breeding is to utilize the photothermal response characteristics of soybeans and the ecological conditions in various regions of China, combined with winter southward breeding, fresh seed direct seeding and molecular marker-assisted selection (MAS) technology to achieve multiple generations of breeding in one year. However, the rapid breeding process of soybeans still cannot get rid of the dependence on natural climate and is difficult to meet the needs of modern agriculture for the breeding of a large number of new varieties.

[0004] For plants, light is not only the energy source that drives plant photosynthesis, but also an important environmental signal that regulates the growth and development processes such as plant flowering and fruiting. Appropriate light environment regulation can accelerate the growth and development process of crops, promote early flowering and rapid fruiting. Therefore, the rapid breeding strategy of crops based on artificial control conditions has been taken seriously by breeders in various countries. As a new type of agricultural production method, the plant factory with high-efficiency and energy-saving LED as the core technology can provide the most suitable light temperature, nutrition and other environmental conditions for crop growth, can greatly shorten the growth period of crops and significantly increase the yield of crops. Under the controllable environment of the plant factory, conducting research on rapid soybean breeding is expected to greatly shorten the time consumed for self-purification of heterozygous soybean materials. However, the lack of LED light formula and its regulation technology for accelerating the soybean breeding process under controllable environment is one of the important industrial problems faced by rapid soybean breeding, and there is an urgent need to construct methods and strategies for LED light environment regulation that can completely get rid of the natural environment and accelerate the soybean crop breeding process. Summary of the Invention

[0005] To solve the above technical problems, the present invention proposes a method for regulating LED light for rapid multiplication and generation acceleration of soybeans to solve the problems existing in the above prior art.

[0006] To achieve the above object, the present invention provides a method for regulating LED light for rapid multiplication and generation acceleration of soybeans, including:

[0007] After the soybean sprouts enter the cotyledon stage, adjust the light quality, light intensity, and photoperiod according to the growth stage of the soybean until the soybean is completely mature. The growth stages of the soybean include the cotyledon stage, the cotyledon stage to the second branching stage, the second branching stage to the flowering and podding stage, the flowering and podding stage to the maturity stage, and the maturity stage.

[0008] Optionally, it also includes pre-treating and germinating the selected soybean seeds, including:

[0009] Evenly spread the soaked soybean seeds on a germination tray lined with wet gauze, add deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap to complete the pre-treatment of the soybean seeds, and germinate them in the dark at 20-25°C for 24-36 hours.

[0010] Optionally, when the growth stage of the soybean is the cotyledon stage, set the photoperiod to 10-13 hours, the light intensity to 200-300 μmol m -2 s -1 , and the light quality is sunlight-like LED white light with a spectrum of 400-780 nm.

[0011] Optionally, when the growth stage of the soybean is from the cotyledon stage to the second branching stage, set the photoperiod to 10-13 hours, the light intensity to 200-400 μmol m -2 s -1 , and the light quality is sunlight-like LED white light with a spectrum of 400-780 nm.

[0012] Optionally, when the growth stage of the soybean is from the second branching stage to the flowering and podding stage, set the light intensity to 200-500 μmolm -2 s -1 , the light cycle to 10-12 hours, and the light quality to sunlight-like LED white light or an LED combined light with a ratio of 1:1. The LED combined light includes three types: red and blue, blue and green, and blue and far red.

[0013] Optionally, when the growth stage of the soybean is from the flowering and podding stage to the maturity stage: set the photoperiod to 14-20 hours, the light quality to sunlight-like LED white light with a spectrum of 400-780 nm, and the light intensity to 300-500 μmol m -2 s -1 .

[0014] Optionally, when the growth stage of the soybean is the maturity stage, set the photoperiod to 24 hours, the light quality to sunlight-like LED white light with a spectrum of 400-780 nm, and the light intensity to 500-700 μmol m -2 s -1 .

[0015] Optionally, when the growth stage of the soybean is the cotyledon stage, from the cotyledon stage to the second branching stage, from the second branching stage to the flowering and podding stage, or from the flowering and podding stage to the maturity stage, the daytime temperature is set to 24-28 °C, the nighttime temperature is set to 20-22 °C, and the air humidity is set to 60-80%.

[0016] Optionally, when the growth stage of the soybean is the maturity stage, the air temperature is set to 30-35 °C and the air humidity is set to 40-60%.

[0017] Compared with the prior art, the present invention has the following advantages and technical effects:

[0018] The present invention provides a method for regulating and controlling the LED light for rapid multiplication and generation addition of soybeans. After the soybeans germinate and enter the cotyledon stage, the light quality, light intensity, and photoperiod are regulated based on the growth stage of the soybeans until the soybeans are completely mature. The growth stages of the soybeans include the cotyledon stage, from the cotyledon stage to the second branching stage, from the second branching stage to the flowering and podding stage, from the flowering and podding stage to the maturity stage, and the maturity stage. The pods harvested after the soybeans mature are dried and then stored at low temperature.

[0019] The present invention discloses an LED light regulation method and a cultivation method for significantly shortening the breeding generation propagation time of dry-cultivated soybeans based on a dynamic LED signal spectrum (green light, far-red light) in a controllable environment. Compared with the traditional simple photoperiod and light intensity regulation methods, the present invention can ensure the germination rate of seeds during the rapid propagation process while significantly shortening the breeding generation propagation time, providing an effective technical approach and method for ensuring a sufficient number of high-quality offspring samples required for the rapid breeding generation purification process of crops in a controllable environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings constituting a part of this application are used to provide a further understanding of this application. The schematic embodiments of this application and their descriptions are used to explain this application and do not constitute an improper limitation of this application. In the drawings:

[0021] Figure 1 It is a flowchart of the method of the embodiment of the present invention;

[0022] Figure 2 It is a statistical analysis chart of the agronomic traits of the rapidly propagated soybeans in Embodiment 1-Embodiment 4 of the embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] It should be noted that, without conflict, the embodiments in this application and the features in the embodiments can be combined with each other. The following will refer to the drawings and combine the embodiments to detail this application.

[0024] It should be noted that the steps shown in the flowchart of the accompanying drawings can be executed in a computer system such as a set of computer-executable instructions. And although the logical order is shown in the flowchart, in some cases, the steps shown or described can be executed in a different order than here.

[0025] Example 1

[0026] As Figure 1 shown, in this example, an LED light formula and cultivation method for accelerating the soybean breeding process are provided, including:

[0027] Seed selection: Preferably select soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0028] Seed soaking: Wash the selected soybean (Huang 13, Williams 82) seeds 3 times with tap water, and soak the seeds in tap water for 12 h at room temperature of 30 °C;

[0029] Seed pretreatment and germination: Evenly spread the soaked soybean seeds on a germination tray lined with wet gauze (15×15×2.0 cm), add 50 mL of deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap, and germinate in the dark at 25 °C for 24 h;

[0030] Cotyledon stage: Sow the germinated seeds into a 72-well (aperture 1.0 cm, hole depth 2.0 cm) seedling tray, add 1.5 cm of tap water to the tray, and transfer it to a cultivation environment with a day / night temperature of 26 °C / 20 °C, an air humidity of 75%, a light cycle of 12 h, and 300 μmol m -2 s -1 simulated sunlight LED white light (sunlike white LED light, W), and the spectrum is 400 - 780 nm for 7 days;

[0031] Management from cotyledon stage to second branch stage: Fix the soybean seedlings with consistent growth vigor with a 5 cm diameter planting sponge block, and transplant them to a 48-well seedling tray. Cultivate them for 7 days under a night temperature of 26 °C / 20 °C, an air humidity of 70%, a light cycle of 12 h, 300 μmol m -2 s -1 simulated sunlight LED white light and 1 / 2 Hoagland nutrient solution;

[0032] Management from second branch stage to flowering and podding stage: When the soybeans enter the second branch stage (V2), transplant the soybean plants to a hydroponic box (12 plants / box, plant spacing 10×10 cm), and under a light intensity of 300 μmol m -2 s -1Cultured under a blue (B, 450 nm) + far-red (Fr, 730 nm) LED light source. During the cultivation process, the day / night temperature, air humidity, and photoperiod were 26°C / 20°C, 70%, and 10 h, respectively. The standard Hoagland nutrient solution of 8 L was replaced every 7 days to ensure the normal water and nutrient requirements of soybeans.

[0033] Flowering and pod-setting stage to maturity stage: After the soybeans entered the flowering and pod-setting stage, a sunlight-like white light LED light source was used. Under a photoperiod of 20 h and a light intensity of 400 μmol m -2 s -1 Cultivated under the conditions of a day / night temperature of 26°C / 20°C and an air humidity of 70%. The Hoagland nutrient solution of 10 L was replaced every 7 days to ensure the normal water and nutrient requirements of hydroponic soybeans.

[0034] Maturity stage: After the soybean grains entered the maturity stage, the nutrient solution irrigation was stopped. A sunlight-like white light LED light source was used. Under a photoperiod of 24 h and a light intensity of 600 μmol m -2 s -1 , an air temperature of 35°C, and an air humidity of 50% for cultivation to promote the rapid ripening of soybean grains.

[0035] Harvest: When the leaves of the soybean plants turned yellow and the soybean grains were completely mature, the pods were harvested and put into kraft paper bags, treated in an oven at 35°C for 48 h, and the soybean grains were collected and stored under low temperature conditions of 4°C.

[0036] In the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 were 26.5 and 30.8 days respectively, the harvesting period was 63.3 days, and the seed germination rate was 85.1%; compared with the field (125 - 127 days), the growth period was advanced by 50.1%, and 5.7 generations of breeding could be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 were 22.3 and 26.7 days respectively, the harvesting period was 62.7 days, and the seed germination rate was 87.2%; compared with the field (140 - 142 days), the growth period was advanced by 55.8%, and 5.8 generations of breeding were expected to be achieved in one year.

[0037] Example 2

[0038] In this example, an LED light formula and cultivation method for accelerating the soybean breeding process are provided, including:

[0039] Seed selection: Select high-quality soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0040] Seed soaking: Wash the selected soybean (Zhonghuang 13, Williams 82) seeds 3 times with tap water and soak the seeds in tap water at room temperature of 25°C for 12 h;

[0041] Seed pretreatment and germination: Evenly place the soaked soybean seeds on a germination tray (15×15×2.0 cm) lined with wet gauze, add 50 mL of deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap, and germinate in the dark at 20 °C for 24 h;

[0042] Cotyledon stage: Sow the germinated seeds into a 72-hole seedling tray (hole diameter 1.0 cm, hole depth 2.0 cm), add 1.5 cm of tap water to the tray, and transfer to a growth environment with a day / night temperature of 26 °C / 20 °C, air humidity of 75%, a photoperiod of 12 h, and 300 μmol m -2 s -1 simulated sunlight white LED light (W), with a spectrum of 400 - 780 nm, and cultivate for 7 days;

[0043] Management from cotyledon stage to second branch stage: Fix the soybean seedlings with consistent growth vigor using a 5-cm diameter planting sponge block, transplant them to a 48-hole seedling tray, and cultivate under conditions of a night temperature of 26 °C / 20 °C, air humidity of 70%, a photoperiod of 12 h, and 300 μmol m -2 s -1 simulated sunlight white LED light and 1 / 2 Hoagland nutrient solution for 7 days.

[0044] Management from second branch stage to flowering and podding stage: When the soybeans enter the second branch stage (V2), transplant the soybean plants to a hydroponic box (12 plants / box, plant spacing 10×10 cm), and cultivate under an LED light source with a light intensity of 300 μmol m -2 s -1 , a light quality ratio of 1:1 red (R, 660 nm) + blue (B, 450 nm). During the cultivation process, the day / night temperature, air humidity, and photoperiod are 26 °C / 20 °C, 70%, and 10 h respectively. Replace 8 L of standard Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of the soybeans.

[0045] Management from flowering and podding stage to maturity stage: After the soybeans enter the flowering and podding stage, use a simulated sunlight white LED light source, with a photoperiod of 20 h, a light intensity of 400 μmol m -2 s -1 , and cultivate under conditions of a day / night temperature of 26 °C / 20 °C and air humidity of 70%. Replace 10 L of Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of the hydroponic soybeans.

[0046] Maturity stage: After the soybean seeds enter the maturity stage, stop irrigating the nutrient solution, use a simulated sunlight white LED light source, with a photoperiod of 24 h and a light intensity of 600 μmol m -2 s-1 Cultivate under the conditions of an air temperature of 35 °C and an air humidity of 50%, which promotes the rapid maturity of soybean grains.

[0047] Harvest: When the leaves of the soybean plants turn yellow and the soybean grains are completely mature, harvest the pods and put them into kraft paper bags, treat them in an oven at 35 °C for 48 h, collect the soybean grains and transfer them to be stored under low temperature conditions of 4 °C.

[0048] In the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 are 25.0 and 28.0 days respectively, the harvesting period is 71.0 days, and the seed germination rate is 94.7%; compared with the field (125 - 127 days), the growth period is advanced by 44.1%, and 5.1 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 21.0 and 22.67 days respectively, the harvesting period is 69.8 days, and the seed germination rate is 94.2%; compared with the field (140 - 142 days), the growth period is advanced by 50.8%, and 5.2 generations of breeding are expected to be achieved in one year.

[0049] Example 3

[0050] In this example, an LED light formula and cultivation method for accelerating the soybean breeding process are provided, including:

[0051] Seed selection: Preferably select soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0052] Seed soaking: Wash the selected soybean (Zhonghuang 13, Williams 82) seeds 3 times with tap water, and soak the seeds in tap water at room temperature of 25 °C for 12 h;

[0053] Seed pretreatment and germination: Evenly spread the soaked soybean seeds on a germination tray lined with wet gauze (15×15×2.0 cm), add 50 mL of deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap, and germinate in the dark at 20 °C for 24 h;

[0054] Cotyledon stage: Sow the germinated seeds into a 72 - hole (aperture 1.0 cm, hole depth 2.0 cm) seedling tray, add 1.5 cm of tap water to the tray, and transfer it to a day / night temperature of 26 °C / 20 °C, air humidity of 75%, light cycle of 12 h, 300 μmol m -2 s -1 Sun - like white LED light (W), with a spectrum of 400 - 780 nm, and cultivate for 7 days;

[0055] Cotyledon stage management to the second branching stage: After fixing soybean seedlings with consistent growth vigor using a planting sponge block with a diameter of 5 cm, transplant them into a 48-well seedling tray, and culture them for 7 days under the conditions of a night temperature of 26°C / 20°C, an air humidity of 70%, a light cycle of 12 h, and a simulated sunlight LED white light with 1 / 2 Hoagland nutrient solution. -2 s -1 class sunlight LED white light, 1 / 2 Hoagland nutrient solution conditions for 7 days.

[0056] Second branching stage to flowering and podding stage management: When soybeans enter the second branching stage (V2), transplant the soybean plants into a hydroponic box (12 plants / box, plant spacing 10×10 cm), and culture them under a light intensity of 300 μmol m -2 s -1 s, and a light quality ratio of 1:1 blue (B, 450 nm) + green (G, 530 nm) LED light source. During the cultivation process, the day / night temperature, air humidity, and light cycle are 26°C / 20°C, 70%, and 10 h respectively. Replace 8 L of standard Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of soybeans.

[0057] Flowering and podding stage to maturity stage: After soybeans enter the flowering and podding stage, use a simulated sunlight white light LED light source, and cultivate them under a light cycle of 20 h and a light intensity of 400 μmol m -2 s -1 with a day / night temperature of 26°C / 20°C and an air humidity of 70%. Replace 10 L of Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of hydroponic soybeans.

[0058] Maturity stage: After the soybean grains enter the maturity stage, stop irrigating the nutrient solution, use a simulated sunlight white light LED light source, and cultivate them under a light cycle of 24 h, a light intensity of 600 μmol m -2 s -1 , an air temperature of 35°C, and an air humidity of 50% to promote the rapid maturity of soybean grains.

[0059] Harvest: When the leaves of the soybean plants turn yellow and the soybean grains are completely mature, harvest the pods and put them into kraft paper bags, treat them in an oven at 35°C for 48 h, collect the soybean grains and transfer them to a 4°C low-temperature condition for storage.

[0060] In the above-mentioned scheme, the initial flowering stage and full-bloom stage of Zhonghuang 13 are 24.7 days and 27.7 days respectively, the harvesting period is 68.7 days, and the seed germination rate is 91.3%; compared with the field (125 - 127 days), the growth period is advanced by 46.6%, and 5.3 generations of breeding can be achieved in one year. The initial flowering stage and full-bloom stage of Williams 82 are 21.0 days and 22.67 days respectively, the harvesting period is 69.8 days, and the seed germination rate is 92.7%; compared with the field (140 - 142 days), the growth period is advanced by 50.1%, and 5.2 generations of breeding are expected to be achieved in one year.

[0061] Example 4

[0062] In this example, an LED light formula and cultivation method for accelerating the soybean breeding process are provided, including:

[0063] Seed selection: Preferably select soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0064] Seed soaking: Wash the selected soybean (Zhonghuang 13, Williams 82) seeds 3 times with tap water, and soak the seeds in tap water for 12 h at room temperature of 30 °C;

[0065] Seed pretreatment and germination acceleration: Evenly place the soaked soybean seeds on a germination tray (15×15×2.0 cm) lined with wet gauze, add 50 mL of deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap, and accelerate germination in the dark at 25 °C for 24 h;

[0066] Cotyledon stage: Sow the germinated seeds into a 72-hole (aperture 1.0 cm, hole depth 2.0 cm) seedling tray, add 1.5 cm of tap water to the tray, and transfer to a day / night temperature of 26 °C / 20 °C, air humidity of 75%, light cycle of 12 h, and 300 μmol m -2 s -1 Sunlike white LED light (W), with a spectrum of 400 - 780 nm, and cultivate for 7 days;

[0067] Management from cotyledon stage to second branch stage: Fix the soybean seedlings with consistent growth vigor using a 5-cm-diameter planting sponge block, transplant them to a 48-hole seedling tray, and cultivate under a night temperature of 26 °C / 20 °C, air humidity of 70%, light cycle of 12 h, 300 μmol m -2 s -1 Sunlike white LED light and 1 / 2 Hoagland nutrient solution for 7 days.

[0068] Management from the second branching stage to the flowering and podding stage: When the soybean enters the second branching stage (V2), transplant the soybean plants into a hydroponic box (12 plants / box, plant spacing 10×10 cm), and cultivate them under a sunlight-like white light LED light source with a light intensity of 300 μmol m -2 s -1 During the cultivation process, the day / night temperature, air humidity, and photoperiod are 26°C / 20°C, 70%, and 10 h respectively. Replace 8 L of standard Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of soybeans.

[0069] From the flowering and podding stage to the maturity stage: After the soybean enters the flowering and podding stage, use a sunlight-like LED white light source, and cultivate it under the conditions of a photoperiod of 20 h, a light intensity of 400 μmol m -2 s -1 The day / night temperature is 26°C / 20°C, and the air humidity is 70%. Replace 10 L of Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of hydroponic soybeans.

[0070] Maturity stage: After the soybean grains enter the maturity stage, stop irrigating the nutrient solution, and use a sunlight-like white light LED light source to cultivate under the conditions of a photoperiod of 24 h, a light intensity of 600 μmol m -2 s -1 , an air temperature of 35°C, and an air humidity of 50% to promote the rapid maturity of soybean grains.

[0071] Harvest: When the leaves of the soybean plants turn yellow and the soybean grains are completely mature, harvest the pods and put them into kraft paper bags, treat them in an oven at 35°C for 48 h, collect the soybean grains and transfer them to be stored under low temperature conditions of 4°C.

[0072] In the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 are 26.7 and 28.7 days respectively, the harvesting period is 75.6 days, and the seed germination rate is 90.7%; compared with the field (125 - 127 days), the growth period is advanced by 40.4%, and 4.8 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 21.6 and 24.0 days respectively, the harvesting period is 73.2 days, and the seed germination rate is 91.8%; compared with the field (140 - 142 days), the growth period is advanced by 48.4%, and 4.9 generations of breeding are expected to be achieved in one year.

[0073] Statistics on the appearance and germination rate of soybean grains accelerated by different LED light formulas in Examples 1 - 4 are as Figure 2As shown in the figure, BFr represents the rapid propagation of soybeans in Example 1, and blue (B, 450 nm) + far-red (Fr, 730 nm) LED light with a light intensity of 300 μmol m-2s-1 was used for treatment from the second branching stage to the flowering and podding stage; BR represents the rapid propagation of soybeans in Example 2, and blue (B, 450 nm) + red (R, 660 nm) LED light with a light intensity of 300 μmol m-2s-1 was used for treatment from the second branching stage to the flowering and podding stage; BG represents the rapid propagation of soybeans in Example 3, and blue (B, 450 nm) + green (G, 530 nm) LED light with a light intensity of 300 μmol m-2s-1 was used for treatment from the second branching stage to the flowering and podding stage; W represents the rapid propagation of soybeans in Example 4, and sunlike white LED light (W; spectrum 400 - 780 nm) with a light intensity of 300 μmol m-2s-1 was used for treatment from the second branching stage to the flowering and podding stage.

[0074] Example 5

[0075] Seed selection: Select soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0076] Seed soaking: Wash the selected soybean seeds (Huang 13, Williams 82) 3 times with tap water and soak the seeds in tap water for 12 h at room temperature of 25 °C;

[0077] Seed pretreatment and germination: Evenly place the soaked soybean seeds on a germination tray lined with wet gauze (15×15×2.0 cm), add 50 mL of deionized water, cover with a layer of wet gauze, seal the germination tray with plastic wrap, and germinate in the dark at 25 °C for 24 h;

[0078] Cotyledon stage: Sow the germinated seeds into a 72-well seedling tray (aperture 1.0 cm, hole depth 2.0 cm), add 1.5 cm of tap water to the tray, and transfer to a day / night temperature of 24 °C / 22 °C, air humidity of 80%, light cycle of 10 h, 200 μmol m -2 s -1 Sunlike white LED light (W), spectrum 400 - 780 nm, and culture for 7 days;

[0079] Management from cotyledon stage to second branching stage: Fix the soybean seedlings with consistent growth on a 5-cm diameter sponge block for planting, transplant them to a 48-well seedling tray, day / night temperature of 28 °C / 22 °C, air humidity of 60%, light cycle of 13 h, 400 μmol m -2 s -1Cultivated under class sunlight LED white light and 1 / 2 Hoagland nutrient solution for 7 days.

[0080] Management from the second branching stage to the flowering and podding stage: When the soybean enters the second branching stage (V2), transplant the soybean plants into a hydroponic box (12 plants / box, plant spacing 10×10 cm), and cultivate under a blue (B, 450 nm) + far red (Fr, 730 nm) LED light source with a light intensity of 200 μmol m -2 s -1 The day / night temperature, air humidity, and photoperiod during the cultivation process are 24°C / 20°C, 80%, and 10 h respectively. Replace 8 L of standard Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of soybeans.

[0081] From the flowering and podding stage to the maturity stage: After the soybean enters the flowering and podding stage, use a class sunlight LED white light source, and cultivate under a photoperiod of 20 h and a light intensity of 400 μmol m -2 s -1 The day / night temperature is 24°C / 20°C and the air humidity is 80%. Replace 10 L of Hoagland nutrient solution every 7 days to ensure the normal water and nutrient requirements of hydroponic soybeans.

[0082] Maturity stage: After the soybean grains enter the maturity stage, stop irrigating the nutrient solution, use a class sunlight LED white light source, and cultivate under a photoperiod of 24 h, a light intensity of 700 μmol m -2 s -1 , an air temperature of 30°C, and an air humidity of 40% to promote the rapid maturity of soybean grains.

[0083] Harvest: When the leaves of the soybean plants turn yellow and the soybean grains are completely mature, harvest the pods and put them into kraft paper bags, treat them in an oven at 35°C for 48 h, collect the soybean grains and transfer them to be stored under 4°C low temperature conditions.

[0084] In the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 are 27.8 and 31.2 days respectively, the harvesting period is 66.4 days, and the seed germination rate is 91.3%; compared with the field (125 - 127 days), the growth period is advanced by 44.7%, and 5.5 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 23.1 and 27.2 days respectively, the harvesting period is 64.4 days, and the seed germination rate is 91.6%; compared with the field (140 - 142 days), the growth period is advanced by 54.6%, and 5.7 generations of breeding are expected to be achieved in one year.

[0085] Example 6

[0086] Seed selection: Select soybean (Glycine max L. Merr) seeds that are complete, plump, and free from pests and diseases;

[0087] Seed soaking: The selected soybean seeds (Huang 13, Williams 82) were washed 3 times with tap water and soaked in tap water for 12 h at room temperature of 30 °C.

[0088] Seed pretreatment and germination promotion: The soaked soybean seeds were evenly spread on a germination tray lined with wet gauze (15×15×2.0 cm), 50 mL of deionized water was added, then covered with a layer of wet gauze, and the germination tray was sealed with plastic wrap and germinated in the dark at 25 °C for 24 h.

[0089] Cotyledon stage: The germinated seeds were sown into a 72-hole seedling tray (aperture 1.0 cm, hole depth 2.0 cm). After adding 1.5 cm of tap water to the tray, it was transferred to a growth environment with a day / night temperature of 26 °C / 20 °C, an air humidity of 75%, a photoperiod of 12 h, and illuminated with 300 μmol m -2 s -1 simulated sunlight LED white light (sunlike white LED light, W), with a spectrum of 400 - 780 nm, and cultured for 7 days.

[0090] Management from cotyledon stage to second branch stage: The soybean seedlings with consistent growth vigor were fixed with a 5-cm-diameter planting sponge block and transplanted into a 48-hole seedling tray. Cultured at a night temperature of 26 °C / 20 °C, an air humidity of 70%, a photoperiod of 12 h, and illuminated with 300 μmol m -2 s -1 simulated sunlight LED white light and under 1 / 2 Hoagland nutrient solution conditions for 7 days.

[0091] Management from second branch stage to flowering and podding stage: When the soybeans entered the second branch stage (V2), the soybean plants were transplanted into a hydroponic box (12 plants / box, plant spacing 10×10 cm), and cultured under a simulated sunlight white light LED light source with a light intensity of 300 μmol m -2 s -1 During the cultivation process, the day / night temperature, air humidity, and photoperiod were 26 °C / 20 °C, 70%, and 10 h respectively. The 8-L standard Hoagland nutrient solution was replaced every 7 days to ensure the normal water and nutrient requirements of the soybeans.

[0092] Management from flowering and podding stage to maturity stage: After the soybeans entered the flowering and podding stage, they were cultured under a simulated sunlight LED white light source with a photoperiod of 14 h and a light intensity of 300 μmol m -2 s -1 at a day / night temperature of 28 °C / 22 °C and an air humidity of 60%. The Hoagland nutrient solution was replaced every 7 days to ensure the normal water and nutrient requirements of the hydroponic soybeans.

[0093] Maturity stage: After the soybean seeds enter the maturity stage, stop irrigating the nutrient solution. Use a white light LED light source similar to sunlight. Cultivate under the conditions of a 24-hour photoperiod, a light intensity of 600 μmol m -2 s -1 , an air temperature of 35 °C, and an air humidity of 60% to promote the rapid maturity of soybean seeds.

[0094] Harvest: When the leaves of the soybean plants turn yellow and the soybean seeds are completely mature, harvest the pods and put them into kraft paper bags. Treat them in an oven at 35 °C for 48 hours, and collect the soybean seeds and store them under low temperature conditions of 4 °C.

[0095] In the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 are 26.4 and 28.9 days respectively, the harvesting period is 77.3 days, and the seed germination rate is 88.9%; compared with the field (125 - 127 days), the growth period is advanced by 39.1%, and 4.7 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 21.6 and 24.0 days respectively, the harvesting period is 75.4 days, and the seed germination rate is 88.2%; compared with the field (140 - 142 days), the growth period is advanced by 46.9%, and 4.8 generations of breeding are expected to be achieved in one year.

[0096] Comparative Example 1

[0097] The difference from Example 5 is only that during the management from the second branching stage to the flower and pod stage, it is cultivated under a blue (B, 450 nm) + far red (Fr, 730 nm) LED light source with a light intensity of 500 μmol m - 2 s -1 .

[0098] In Comparative Example 1, in the above scheme, the initial flowering stage and full flowering stage of Zhonghuang 13 are 24.2 and 27.6 days respectively, the harvesting period is 62.1 days, and the seed germination rate is 89.2%; compared with the field (125 - 127 days), the growth period is advanced by 51.1%, and 5.8 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 21.1 and 23.3 days respectively, the harvesting period is 61.5 days, and the seed germination rate is 90.3%; compared with the field (140 - 142 days), the growth period is advanced by 55.8%, and 5.9 generations of breeding are expected to be achieved in one year.

[0099] Comparative Example 2

[0100] The difference from Example 5 is only that the light quality regulation is omitted during the management from the second branching stage to the flower and pod stage.

[0101] In Comparative Example 2, the initial flowering stage and full-bloom stage of Zhonghuang 13 were 28.4 days and 30.5 days respectively, the harvesting period was 77.1 days, and the seed germination rate was 90.3%; compared with the field (125 - 127 days), the growth period was advanced by 39.3%, and 4.7 generations of breeding could be achieved in one year. The initial flowering stage and full-bloom stage of Williams 82 were 23.9 days and 26.2 days respectively, the harvesting period was 75.1 days, and the seed germination rate was 90.5%; compared with the field (140 - 142 days), the growth period was advanced by 47.1%, and 4.9 generations of breeding were expected to be achieved in one year.

[0102] Comparative Example 3

[0103] The difference from Example 5 was only that light quality regulation was omitted during the management from the second branching stage to the flowering and podding stage, and light regulation was omitted from the flowering and podding stage to the maturity stage.

[0104] In Comparative Example 3, the initial flowering stage and full-bloom stage of Zhonghuang 13 were 28.2 days and 31.0 days respectively, the harvesting period was 79.2 days, and the seed germination rate was 89.7%; compared with the field (125 - 127 days), the growth period was advanced by 37.6%, and 4.6 generations of breeding could be achieved in one year. The initial flowering stage and full-bloom stage of Williams 82 were 23.5 days and 26.9 days respectively, the harvesting period was 77.4 days, and the seed germination rate was 90.1%; compared with the field (140 - 142 days), the growth period was advanced by 45.4%, and 4.7 generations of breeding were expected to be achieved in one year.

[0105] Comparative Example 4

[0106] Same as Example 1, the difference was only that after the cotyledon stage, the germinated soybean seeds were directly sown into a cultivation box filled with 8 L of mixed substrate (peat: perlite = 3:1), and the plant spacing was 10×10 cm. After emergence, from the cotyledon stage management to the second branching stage to the maturity stage, substrate cultivation was adopted throughout. Every 3 days, 4 L of Hoagland nutrient solution was poured into each cultivation box to ensure the water and nutrient requirements for soybean growth.

[0107] In Comparative Example 4, the initial flowering stage and full-bloom stage of Zhonghuang 13 were 35.7 days and 40.1 days respectively, the harvesting period was 86.3 days, and the seed germination rate was 88.3%; compared with the field (125 - 127 days), the growth period was advanced by 32.1%, and 4.2 generations of breeding could be achieved in one year. The initial flowering stage and full-bloom stage of Williams 82 were 31.5 days and 37.4 days respectively, the harvesting period was 84.8 days, and the seed germination rate was 88.2%; compared with the field (140 - 142 days), the growth period was advanced by 40.1%, and 4.3 generations of breeding were expected to be achieved in one year.

[0108] Compared with the method in Example 1, in Comparative Example 4, the post-harvest time of Zhonghuang 13 and Williams 82 was postponed by 23 days and 22 days respectively, and 1.5 generations less were bred in one year.

[0109] Comparative Example 5

[0110] Same as Example 2, with the only difference being that after germination acceleration, the soybean seeds are directly sown into a cultivation box filled with 8 L of mixed substrate (peat: perlite = 3:1) during the cotyledon stage, and the plant spacing is 10×10 cm. After emergence, substrate cultivation is adopted throughout the whole process from the cotyledon stage to the second branching stage and maturity stage. Every 3 days, 4 L of Hoagland nutrient solution is poured into each cultivation box to ensure the water and nutrient requirements for soybean growth.

[0111] In Comparative Example 5, the initial flowering stage and full flowering stage of Zhonghuang 13 are 32.4 days and 37.2 days respectively, the harvesting period is 87.3 days, and the seed germination rate is 92.1%; compared with the field (125 - 127 days), the growth period is advanced by 31.2%, and 4.2 generations of breeding can be achieved in one year. The initial flowering stage and full flowering stage of Williams 82 are 31.5 days and 37.4 days respectively, the harvesting period is 85.4 days, and the seed germination rate is 93.4%; compared with the field (140 - 142 days), the growth period is advanced by 40.4%, and 4.3 generations of breeding are expected to be achieved in one year.

[0112] Compared with the method in Example 2, the post - harvest time of Zhonghuang 13 and Williams 82 in Comparative Example 5 is delayed by 18.7 days and 16.4 days respectively, and 1.0 generation less breeding can be achieved in one year.

[0113] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the technical field within the technical scope disclosed in the present application should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method for LED light regulation of soybean rapid propagation, characterized in that: The following steps are involved: After the soybeans germinate and enter the cotyledon stage, the light quality, light intensity and light cycle are adjusted according to the growth stage of the soybeans until the soybeans are fully mature; the growth stages of the soybeans include the cotyledon stage, the cotyledon stage to the second branch stage, the second branch stage to the flowering and pod stage, the flowering and pod stage to the maturity stage, and the maturity stage; When soybeans are in the cotyledon stage, the photoperiod is set to 10-13 h and the light intensity is set to 200-300 μmol m -2 s -1 , the light quality is sunlight-like LED white light, and the spectrum is 400~780 nm; When the growth stage of soybean was from cotyledon stage to second branching stage, the photoperiod was set to 10-13 h and the light intensity was set to 200-400 μmol m -2 s -1 , the light quality is sunlight-like LED white light, and the spectrum is 400~780 nm; When the soybean growth stage is from the second branching stage to the flowering and pod stage, the light intensity is set at 200-500 μmol m -2 s -1 , the photoperiod is 10-12 h, the light quality is sunlight-like LED white light or LED combination light with a ratio of 1:1, and the LED combination light includes red and blue, blue and green, and blue and far-red; When the soybean growth stage is from the flowering and pod stage to the maturity stage, the photoperiod is set to 14-20 h, the light quality is sunlight-like LED white light, the spectrum is 400-780 nm, and the light intensity is 300-500 μmol m -2 s -1 ; When the soybean growth stage was mature, the photoperiod was set to 24 h, the light quality was sunlight-like LED white light, the spectrum was 400-780 nm, and the light intensity was 500-700 μmol m -2 s -1 ; When the growth stages of soybean are cotyledon stage, cotyledon stage to second branch stage, second branch stage to flowering and pod stage, or flowering and pod stage to maturity stage, set the daytime temperature to 24-28 ℃, the nighttime temperature to 20-22 ℃, and the air humidity to 60-80%; When the soybean growth stage is mature, set the air temperature to 30~35℃ and the air humidity to 40~60%.

2. The LED light control method for soybean rapid propagation according to claim 1, characterized in that: The method also includes pre-treatment and germination of the selected soybean seeds, including: Spread the soaked soybean seeds evenly on a germination tray covered with wet gauze, add deionized water and cover with a layer of wet gauze, seal the germination tray with plastic wrap to complete the pretreatment of the soybean seeds, and germinate them in a dark environment at 20-25 ℃ for 24-36 hours.

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