A pineapple plant growth regulating composition and its regulator

Abstract

This invention provides a pineapple plant growth regulator composition and its regulator, relating to the field of plant growth regulators. The pineapple plant growth regulator composition of this invention comprises the following raw materials in parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, and 10-20 parts of jasmonic acid. This invention uses calcium diphenylureasulfonate, titanium citrate, N6-isopentenyladenine, 4-iodophenoxyacetic acid, and jasmonic acid as core components to prepare a pineapple plant growth regulator composition, which not only significantly increases the single fruit weight of pineapple but also improves fruit quality and effectively reduces the incidence of watercore disease. The plant growth regulator of this invention can enhance the pineapple's defense response against watercore disease, while promoting growth, improving its stress resistance, and increasing fruit quality and yield, resulting in significant benefits in production.

Description

Technical Field

[0001] This invention relates to the field of plant growth regulators, and particularly to a pineapple plant growth regulator composition and its regulator. Background Technology

[0002] Plant growth regulators can promote root development, flowering, and fruiting in crops, and are of great value in optimizing crop growth and improving crop yield and quality.

[0003] Pineapple (Ananas comosus L., Merr.) is one of the most important fruits in tropical regions. Watercore pineapple is a physiological disorder that begins to appear in the later stages of fruit development (2-4 weeks before harvest), causing the flesh of the affected fruit to become waterlogged. Because the intercellular spaces of the flesh are filled with fluid, watercore pineapples emit a fermented or foul odor as the disease progresses. Severe watercore can completely render the pineapple unmarketable. Studies have shown that changes in the content of endogenous hormones in the fruit can affect the occurrence of watercore; an increase in gibberellin can exacerbate the severity of watercore and negatively impact fruit quality.

[0004] Therefore, researching a new plant growth regulator for pineapples, which can reduce the incidence of watercore disease and improve the quality and yield of pineapples, is of great significance. Summary of the Invention

[0005] In view of this, the present invention proposes a pineapple plant growth regulating composition and its regulator to solve the above problems.

[0006] The technical solution of this invention is implemented as follows:

[0007] A pineapple plant growth regulator composition comprising the following raw materials in parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, and 10-20 parts of jasmonic acid.

[0008] Preferably, the pineapple plant growth regulator composition comprises the following raw materials in parts by weight: 35-45 parts of calcium diphenylureasulfonate, 17-19 parts of titanium citrate, 9-11 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, and 13-17 parts of jasmonic acid.

[0009] Preferably, the pineapple plant growth regulator composition comprises the following raw materials in parts by weight: 40 parts of calcium diphenylureasulfonate, 18 parts of titanium citrate, 10 parts of N6-isopentenyladenine, 4 parts of 4-iodophenoxyacetic acid, and 15 parts of jasmonic acid.

[0010] Preferably, the pineapple plant growth regulating composition further contains sodium diisooctyl succinate sulfonate.

[0011] Preferably, the pineapple plant growth regulating composition further contains cyclodextrin, which is composed of β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:4-5.

[0012] Preferably, the pineapple plant growth regulator composition further contains acetone and water, comprising the following raw materials in parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, 10-20 parts of jasmonic acid, 6-8 parts of sodium diisooctyl succinate sulfonate, 0.4-0.6 parts of cyclodextrin, 5-10 parts of acetone, and 90-110 parts of water.

[0013] Preferably, the pineapple plant growth regulator composition comprises the following raw materials in parts by weight: 40 parts of calcium diphenylureasulfonate, 18 parts of titanium citrate, 10 parts of N6-isopentenyladenine, 4 parts of 4-iodophenoxyacetic acid, 25 parts of jasmonic acid, 7 parts of sodium diisooctyl succinate sulfonate, 0.5 parts of cyclodextrin, 8 parts of acetone, and 100 parts of water.

[0014] Preferably, the preparation method of the pineapple plant growth regulator composition includes the following steps: adding calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stirring at 40-45°C for 20-40 min, adding 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stirring at 35-40°C for 10-20 min, and finally adding N6-isopentenyladenine, stirring at 20-30°C for 5-15 min to obtain the product.

[0015] Preferably, the stirring speed is 400-500 rpm.

[0016] A pineapple plant growth regulator comprising the composition described in any one of the present invention.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] (1) The present invention uses calcium diphenylureasulfonate, titanium citrate, N6-isopentenyladenine, 4-iodophenoxyacetic acid and jasmonic acid as core active components to prepare a pineapple plant growth regulator composition, which can not only significantly increase the single fruit weight of pineapple, but also improve the fruit quality and effectively reduce the incidence of watercore disease.

[0019] (2) The plant growth regulator of the present invention can enhance the pineapple’s defense response to watercore disease, while promoting growth, improving its stress resistance, improving fruit quality and yield, and achieving significant benefits in production.

[0020] (3) The present invention adds a certain proportion of β-cyclodextrin and γ-cyclodextrin as well as sodium diisooctyl succinate sulfonate to promote the hydrophilic interaction between the components of the system, effectively improve the stability of the product, and effectively promote the efficacy of the active components.

[0021] (4) The preparation process of the plant growth regulator of the present invention reduces the amount of organic solvent used to a large extent while ensuring product stability and product effect, and has the characteristics of being environmentally friendly and efficient. Detailed Implementation

[0022] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0023] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0024] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0025] Example 1

[0026] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0027] Table 1 Formulation of Pineapple Plant Growth Regulator Composition

[0028] Components Dosage (kg) Calcium diphenylureasulfonate 40.0 Titanium citrate (IV) 18.0 N6-Isopentenyladenine 10.0 4-Iodophenoxyacetic acid 4.0 Jasmonic acid 15.0 Sodium diisooctyl succinate sulfonate 7.0 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 0.5 acetone 5.0 water 100.0

[0029] 2. Preparation method of pineapple plant growth regulator

[0030] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0031] Example 2

[0032] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0033] Table 2 Formulation of Pineapple Plant Growth Regulator Composition

[0034] Components Dosage (kg) Calcium diphenylureasulfonate 50.0 Titanium citrate (IV) 15.0 N6-Isopentenyladenine 8.0 4-Iodophenoxyacetic acid 5.0 Jasmonic acid 15.0 Sodium diisooctyl succinate sulfonate 8.0 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 0.4 acetone 5.0 water 100.0

[0035] 2. Preparation method of pineapple plant growth regulator

[0036] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0037] Example 3

[0038] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0039] Table 3 Formulation of Pineapple Plant Growth Regulator Composition

[0040]

[0041]

[0042] 2. Preparation method of pineapple plant growth regulator

[0043] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0044] Example 4

[0045] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0046] Table 4 Formulation of Pineapple Plant Growth Regulator Composition

[0047] Components Dosage (kg) Calcium diphenylureasulfonate 47.0 Titanium citrate (IV) 15.0 N6-Isopentenyladenine 10.0 4-Iodophenoxyacetic acid 5.0 Jasmonic acid 10.0 Sodium diisooctyl succinate sulfonate 7.0 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 0.5 acetone 5.0 water 100.0

[0048] 2. Preparation method of pineapple plant growth regulator

[0049] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0050] Comparative Example 1

[0051] The main difference between this example and Example 1 is that jasmonic acid was not added to the formula, and the amount of 4-iodophenoxyacetic acid was increased.

[0052] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0053] Table 5. Formulation of Pineapple Plant Growth Regulator Composition

[0054] Components Dosage (kg) Calcium diphenylureasulfonate 40 Titanium citrate (IV) 18 N6-Isopentenyladenine 10 4-Iodophenoxyacetic acid 19 Jasmonic acid 0 Sodium diisooctyl succinate sulfonate 7 β-Cyclodextrin 0.5 acetone 5 water 100

[0055] 2. Preparation method of pineapple plant growth regulator

[0056] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0057] Comparative Example 2

[0058] The main difference between this example and Example 1 is that titanium citrate was not added to the formulation, and the amount of calcium diphenylureasulfonate was increased.

[0059] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0060] Table 6 Formulation of Pineapple Plant Growth Regulator Composition

[0061]

[0062]

[0063] 2. Preparation method of pineapple plant growth regulator

[0064] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0065] Comparative Example 3

[0066] The main difference between this example and Example 1 is that N6-isopentenyladenine was not added to the formulation, and the amount of titanium citrate (Ⅳ) was increased.

[0067] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0068] Table 7 Formulation of Pineapple Plant Growth Regulator Composition

[0069] Components Dosage (kg) Calcium diphenylureasulfonate 40 Titanium citrate (IV) 28 N6-Isopentenyladenine 0 4-Iodophenoxyacetic acid 4 Jasmonic acid 15 Sodium diisooctyl succinate sulfonate 7 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 0.5 acetone 5 water 100

[0070] 2. Preparation method of pineapple plant growth regulator

[0071] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally cool to 25℃±2℃ and stir at 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0072] Comparative Example 4

[0073] Compared with Example 1, the amounts of calcium diphenylureasulfonate, titanium citrate, N6-isopentenyladenine, 4-iodophenoxyacetic acid, and jasmonic acid added in this example are different.

[0074] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0075] Table 8 Formulation of Pineapple Plant Growth Regulator Composition

[0076] Components Dosage (kg) Calcium diphenylureasulfonate 22.0 Titanium citrate (IV) 30.0 N6-Isopentenyladenine 20.0 4-Iodophenoxyacetic acid 10.0 Jasmonic acid 5.0 Sodium diisooctyl succinate sulfonate 7.0 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 0.5 acetone 5.0 water 100.0

[0077] 2. Preparation method of pineapple plant growth regulator

[0078] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0079] Comparative Example 5

[0080] Compared to Example 1, this example uses only β-cyclodextrin and does not include γ-cyclodextrin.

[0081] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0082] Table 9 Formulation of Pineapple Plant Growth Regulator Composition

[0083]

[0084]

[0085] 2. The preparation method of the pineapple plant growth regulator is the same as that in Example 1.

[0086] Comparative Example 6

[0087] Compared with Example 1, this example reduces the amount of sodium diisooctyl succinate sulfonate added and increases the amount of cyclodextrin added.

[0088] 1. Formulation of Pineapple Plant Growth Regulator Composition

[0089] Table 10 Formulation of Pineapple Plant Growth Regulator Composition

[0090] Components Dosage (kg) Calcium diphenylureasulfonate 40.0 Titanium citrate (IV) 18.0 N6-Isopentenyladenine 10.0 4-Iodophenoxyacetic acid 4.0 Jasmonic acid 15.0 Sodium diisooctyl succinate sulfonate 5.0 Cyclodextrin (β-cyclodextrin + γ-cyclodextrin = 1:4, w / w) 2.0 acetone 5.0 water 100.0

[0091] 2. Preparation method of pineapple plant growth regulator

[0092] Specific steps: According to the formula table of pineapple plant growth regulator composition, weigh out each component raw material separately, add calcium diphenylurea sulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stir at 42℃±2℃ and 450rpm±50rpm for 30min, add 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stir at 37℃±2℃ and 450rpm±50rpm for 15min, and finally add N6-isopentenyladenine, stir at 25℃±2℃ and 450rpm±50rpm for 10min to obtain pineapple plant growth regulator.

[0093] Test case

[0094] Biological material: Golden pineapple, variety MD-2 Golden Pineapple.

[0095] The experiment was conducted at the Sanya Pineapple Experiment Base in Hainan. After the golden pineapple entered the reddening stage, plants with uniform growth and consistent development were selected, with a plant height of 80-90cm and 45-50 functional leaves.

[0096] Plant growth regulators: The plant growth regulators prepared in Examples 1-4 and Comparative Examples 1-6 were diluted 1000 times with water and then sprayed. A blank control (water) was set up. Each treatment was set up in 3 replicates, with 100 plants in each replicate, that is, 300 plants in each treatment.

[0097] See Table 1 for spraying timing. Spray the young pineapple fruits in the afternoon (on cloudy or overcast days), ensuring even coverage. If it rains within 24 hours of application, re-spray. Harvest the fruit on the day when approximately 50% of the fruit reaches the harvest standard.

[0098] Table 11 Timing of Plant Growth Regulator Spraying

[0099] Timing of spraying Spraying amount (raw material) 1st time 7 days after the redness appears Approximately 2ml / plant 2nd time 7 days after the first spraying Approximately 5ml / plant 3rd time 10 days after the second spraying Approximately 8m1 / plant 4th 7 days after the third spraying Approximately 10ml / plant

[0100] After harvest, the incidence of watercore disease in golden pineapple fruits was counted. If the disease severity reached moderate or above, the pineapple with watercore disease (i.e., the area of ​​watercore lesions accounted for ≥23% of the whole fruit area) would have a fermented smell or even rotten and foul odor, thus losing its commercial value.

[0101] Table 12 Effects of plant growth regulators on watercore disease in pineapple fruits

[0102] Group Incidence of watercore disease (moderate to high) CK 10.3％ Example 1 0.3％ Example 2 1.0％ Example 3 0.7％ Example 4 1.3％ Comparative Example 1 5.3％ Comparative Example 2 7.0％ Comparative Example 3 4.3％ Comparative Example 4 3.3％ Comparative Example 5 2.3％ Comparative Example 6 1.7％

[0103] As shown in Table 12, compared with the control group (CK), the incidence of moderate to severe hydrocardia in Examples 1-4 and Comparative Examples 1-6 was reduced to a certain extent (p<0.05), with the incidence of hydrocardia in Examples 1-4 being lower.

[0104] After harvest, the weight of individual golden pineapple fruits that had not lost their commercial value was tallied, and fruit quality was tested, including soluble sugar and vitamin C content. Soluble sugar was measured using a pocket digital refractometer, and vitamin C was measured according to GB5009.86-2016.

[0105] Table 13 Effects of plant growth regulators on the single fruit weight of Pineapple (Golden Pineapple)

[0106] Group Single fruit weight (g) Weight gain rate (mm) CK 1209.4±31.1 -- Example 1 1914.6±16.9 58.31％ Example 2 1837.2±19.4 51.91％ Example 3 1791.4±22.6 48.12％ Example 4 1848.3±24.3 52.83％ Comparative Example 1 1481.2±20.3 22.47％ Comparative Example 2 1537.1±25.4 27.10％ Comparative Example 3 1598.5±26.2 32.17％ Comparative Example 4 1625.9±20.8 34.44％ Comparative Example 5 1662.7±18.2 37.48％ Comparative Example 6 1700.2±17.0 40.58％

[0107] As shown in Table 13, compared with the control group (CK), the single fruit weight of golden pineapple in Examples 1-4 and Comparative Examples 1-6 was increased (p<0.05), with the weight gain rate of Examples 1-4 being higher.

[0108] Table 14 Effects of plant growth regulators on the fruit quality of Pineapple

[0109] Group Soluble sugars (mg / g) Vitamin C (mg / 100g) CK 157.13±4.67 24.08±0.53 Example 1 174.10±4.18 28.50±0.26 Example 2 173.92±4.83 27.00±0.34 Example 3 174.47±4.25 27.56±0.27 Example 4 172.28±3.85 26.75±0.38 Comparative Example 1 157.11±3.52 25.09±0.62 Comparative Example 2 153.19±4.17 25.66±0.59 Comparative Example 3 155.81±3.91 25.04±0.65 Comparative Example 4 159.78±3.68 25.72±0.72 Comparative Example 5 168.20±4.02 26.63±0.61 Comparative Example 6 167.69±4.27 26.28±0.47

[0110] As shown in Table 14, compared with the control group (CK), the soluble sugar and vitamin C content of the golden pineapple fruit in Examples 1-4 and Comparative Examples 1-6 were all increased (p<0.05), with the fruit quality of Examples 1-4 being better.

[0111] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A pineapple plant growth regulator composition, characterized in that, The raw materials include the following parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, 10-20 parts of jasmonic acid, 6-8 parts of sodium diisooctyl succinate sulfonate, 0.4-0.6 parts of cyclodextrin, 5-10 parts of acetone, and 90-110 parts of water, wherein the cyclodextrin is composed of β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:4-5.

2. The pineapple plant growth regulator composition according to claim 1, characterized in that, The raw materials include the following parts by weight: 40 parts calcium diphenylurea sulfonate, 18 parts titanium citrate, 10 parts N6-isopentenyl adenine, 4 parts 4-iodophenoxyacetic acid, 15 parts jasmonic acid, 7 parts sodium diisooctyl succinate sulfonate, 0.5 parts cyclodextrin, 8 parts acetone, and 100 parts water.

3. The pineapple plant growth regulator composition according to claim 1, characterized in that, The preparation method of the composition includes the following steps: adding calcium diphenylureasulfonate, titanium citrate, and sodium diisooctyl succinate sulfonate to acetone and water, stirring at 40-45°C for 20-40 min, adding 4-iodophenoxyacetic acid, jasmonic acid, and cyclodextrin, stirring at 35-40°C for 10-20 min, and finally adding N6-isopentenyladenine, stirring at 20-30°C for 5-15 min to obtain the composition.

4. The pineapple plant growth regulating composition according to claim 3, characterized in that, The stirring speed is 400-500 rpm.

5. A pineapple plant growth regulator, characterized in that, Contains the composition according to any one of claims 1-3.

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