A moisturizing and repairing bath oil

Abstract

The present invention discloses a moisturizing and repairing bath oil, belonging to the technical field of daily chemical products. Its raw materials include PEG-5 brassinolide, lanolin oil, betula oil, michelia alba oil, macadamia nut oil, babassu oil, vanilla planifolia oil, a mixed fermentation extract of borage seeds and coix seeds, wherein the mixed fermentation extract of borage seeds and coix seeds is fermented with borage seeds and coix seeds as raw materials and trichoderma reesei as the fermentation strain. The bath oil provided by the present invention has remarkable effects of cleaning, moisturizing, repairing, antioxidant and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemical products, and particularly relates to a moisturizing and repairing bath oil. Background Art

[0002] With the acceleration of the modern life rhythm, consumers' awareness of health and beauty has increased. People's demands for bath products have become increasingly diverse. They not only pursue moderate foam and excellent cleaning effects, but also pay more attention to the mildness of the product during use and the skin nourishing effect after use, avoiding irritation or damage to the skin. In recent years, the concept of "nourishing the skin with oil" has become increasingly popular among consumers in the Chinese beauty market. They are more willing to try various oil products, such as makeup remover oil, skin moisturizing oil, body oil, and massage oil, etc. These products show great growth potential in the market, and bath oil is one of the best.

[0003] Traditional bath products are mainly divided into two categories: one is soap-based body wash, which is made by the high-temperature saponification reaction of fatty acids and strong alkalis (such as potassium hydroxide or sodium hydroxide), or compounded with other ingredients. Although this type of body wash has strong cleaning power, it has a strong smell, high alkalinity, and excessive degreasing power, which easily leads to problems such as dryness, tightness, and itching of the skin after bathing. The other is surfactant-based body wash, which is compounded by various surfactants. Some surfactants have strong degreasing power, and after washing, it may not feel refreshing enough and there may be a residue feeling. Long-term use may also damage the sebum film on the skin surface, weaken the natural barrier of the skin, make the skin become fragile and dry, and easily cause various skin problems. Therefore, after bathing, skin care products such as body lotion or skin moisturizing oil are often needed to relieve these skin problems.

[0004] Under such circumstances, bath oil containing oil components has gradually become a new choice for people's daily washing and care. With its mild cleaning power and good nourishing effect, it is loved by more and more consumers. In recent years, the market share of bath oil has been continuously expanding. However, bath oil also has some problems, such as paying attention to moisturizing and cleaning power while ignoring the repair and nourishment of the skin. Therefore, there is an urgent need to provide a bath oil prepared from natural plant extracts, which has multiple functions such as moisturizing, moisturizing, and repairing while having basic cleaning ability. Summary of the Invention

[0005] The present invention provides a moisturizing and repairing bath oil, which plays a role in skin moisturizing and soothing through natural ingredients extracted from plants.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] In the first aspect, the present invention provides a preparation method of a mixed fermentation extract, and its steps are specifically as follows:

[0008] S1: Take dry borage seeds and adlay seeds, grind and crush them, and then soak them in water for 12h - 16h.

[0009] S2: Take the suspension after soaking in S1 for 12 hours, sterilize it at high temperature, wait until it cools to room temperature, inoculate Trichoderma reesei, ferment for 3 - 4 days, the initial fermentation temperature is 28 - 30°C, and then inactivate and filter to obtain filtrate A.

[0010] S3: Freeze-dry filtrate A to obtain the mixed fermentation extract of borage seeds and adlay seeds.

[0011] Preferably, the mass fraction ratio of borage seeds to adlay seeds in S1 is 1:2 - 3; the mass percentage of Trichoderma reesei in S2 accounts for 2 - 4% of the total mass of borage seeds and adlay seeds.

[0012] In the second aspect, the present invention provides an active composition, and its specific raw material mass composition is as follows:

[0013] PEG - 5 brassinolide 0.3 - 0.6 parts;

[0014] Lanolin oil 1 - 2 parts;

[0015] Betula oil 2 - 4 parts;

[0016] Michelia alba oil 1 - 3 parts;

[0017] Macadamia nut oil 1 - 2 parts;

[0018] Babassu oil 1 - 2 parts;

[0019] Vanilla planifolia oil 0.5 - 0.9 parts;

[0020] The mixed fermentation extract of borage seeds and adlay seeds 0.1 - 0.5 parts.

[0021] In the third aspect, the present invention provides a bath oil, and its specific raw material mass composition is as follows:

[0022] Active composition 1 - 5wt%;

[0023] Emulsifier 10 - 30wt%;

[0024] Surfactant 15 - 40wt%;

[0025] Humectant 0.5 - 5wt%;

[0026] Antioxidant 0.01 - 0.3wt%.

[0027] The balance is made up with base oil.

[0028] The base oil can be one or more of jojoba seed oil, camellia seed oil, Korean pine seed oil, and sunflower seed oil; the emulsifier can be one or more of lauryl polyether-4 and PEG-25 hydrogenated castor oil; the surfactant can be one or more of MIPA lauryl polyether sulfate and stearamidopropyl dimethylamine; the humectant can be one or more of propylene glycol and glycerin; the antioxidant is pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamate).

[0029] In the present invention: Trichoderma reesei is innovatively used to ferment borage seeds and adlay seeds, so as to increase the volatile substances therein, such as sterols, furans, alcohols, ketones, alkanes, etc., which have significant antioxidant, antibacterial, moisturizing and other effects on the skin. Through the compounding of the mixed fermentation extract of borage seeds and adlay seeds with PEG-5 brassicasterol, lanolin oil, betulin oil, Michelia alba oil, macadamia seed oil, babassu oil, and vanilla planifolia oil, through the compounding effect among various plant extracts, synergistic effects are achieved, making it have significant moisturizing, antioxidant, and antibacterial effects on the skin.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0031] 1. By using Trichoderma reesei to ferment the mixture of borage seeds and adlay seeds, the volatile substances in the borage seeds and adlay seeds are enriched, and new volatile substances are generated.

[0032] 2. By compounding the mixed fermentation product of borage seeds and adlay seeds with PEG-5 brassicasterol, lanolin oil, betulin oil, Michelia alba oil, macadamia seed oil, babassu oil, and vanilla planifolia oil, synergistic effects are achieved among the plant extracts, making the composition have significant moisturizing, antioxidant, and antibacterial effects on the skin. Detailed implementation manners

[0033] To better understand the present invention, the following specific embodiments are used to further describe the present invention. The terms used in the embodiments are for describing specific implementation manners and do not constitute a limitation on the protection scope of the present invention.

[0034] For the experimental methods without specific conditions indicated in the following embodiments, they are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are calculated by weight.

[0035] The sources of some raw materials are as follows:

[0036] PEG-5 brassicasterol: purchased from Shanghai Yuanye Bio-Technology Co., Ltd.

[0037] Lanolin oil: purchased from Shanghai Yuanye Bio-Technology Co., Ltd.

[0038] Betula platyphylla Suk. oil: Purchased from Wuhan Kemic Biopharmaceutical Technology Co., Ltd.

[0039] Michelia alba DC. oil: Purchased from Shanghai Macklin Biochemical Co., Ltd.

[0040] Macadamia ternifolia F. Muell. seed oil: Purchased from Hubei Yamaide Biopharmaceutical Co., Ltd.

[0041] Orbignya phalerata Mart. oil: Purchased from Guangdong Yunxing Biotechnology Co., Ltd.

[0042] Vanilla planifolia Andrews oil: Purchased from Wuhan Wande Chemical Co., Ltd.

[0043] Borago officinalis L. seeds: Purchased from Shuyang Tianzhiqu Landscape Greening Engineering Co., Ltd.

[0044] Coix lacryma-jobi L. seeds: Purchased from Bozhou Tongxintang Biotechnology Co., Ltd.

[0045] Trichoderma reesei: Purchased from Ningbo Mingzhou Biotechnology Co., Ltd., with the number BMZ115313.

[0046] Trichoderma viride: Purchased from Ningbo Mingzhou Biotechnology Co., Ltd., with the number BMZ127533.

[0047] Borago officinalis L. seed oil: Purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.

[0048] Coix lacryma-jobi L. seed oil: Purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.

[0049] Preparation, including the following steps:

[0050] Preparation steps of the mixed fermentation product of Borago officinalis L. seeds and Coix lacryma-jobi L. seeds are as follows:

[0051] S1: Take the dried Borago officinalis L. seeds and Coix lacryma-jobi L. seeds, grind and crush them, and then soak them in water for 12h - 16h.

[0052] S2: Take the suspension after soaking in S1 for 12 hours, sterilize it at high temperature, wait until it cools to room temperature, inoculate Trichoderma reesei, ferment for 3 - 4 days, the initial fermentation temperature is 28 - 30 °C, and then inactivate and filter to obtain filtrate A.

[0053] S3: Freeze-dry filtrate A to obtain the mixed fermentation extract of Borago officinalis L. seeds and Coix lacryma-jobi L. seeds.

[0054] Example 1: Comply with the preparation process of S1 - S3. In S1, the mass fraction ratio of borage seeds to adlay seeds is 1:2.5; soak in water for 14 h. In S2, the mass percentage of Trichoderma reesei accounts for 3% of the total mass of borage seeds and adlay seeds; the fermentation time is 4 days, and the initial fermentation temperature is 29 °C.

[0055] Example 2: Comply with the preparation process of S1 - S3. In S1, the mass fraction ratio of borage seeds to adlay seeds is 1:2; soak in water for 12 h. In S2, the mass percentage of Trichoderma reesei accounts for 5% of the total mass of borage seeds and adlay seeds; the fermentation time is 4 days, and the initial fermentation temperature is 28 °C.

[0056] Example 3: Comply with the preparation process of S1 - S3. In S1, the mass fraction ratio of borage seeds to adlay seeds is 1:3; soak in water for 16 h. In S2, the mass percentage of Trichoderma reesei accounts for 4% of the total mass of borage seeds and adlay seeds; the fermentation time is 3 days, and the initial fermentation temperature is 30 °C.

[0057] Comparative Example 1: In S1, change borage seeds and adlay seeds to only borage seeds, and the rest is the same as Example 1.

[0058] Comparative Example 2: In S1, change borage seeds and adlay seeds to only adlay seeds, and the rest is the same as Example 1.

[0059] Comparative Example 3: In S2, change Trichoderma reesei to Trichoderma viride, and the rest is the same as Example 1.

[0060] An active composition, and the specific mass parts composition of raw materials is shown in Table 1 in detail.

[0061] Table 1

[0062]

[0063]

[0064] A bath oil, and the specific raw materials for preparation are shown in Table 2;

[0065] Table 2

[0066]

[0067] Note: The emulsifier is: the mass fraction ratio of laureth - 4 to PEG - 25 hydrogenated castor oil is 1:0.7; the surfactant is: MIPA lauryl polyether sulfate; the humectant is: glycerol; the antioxidant is: pentaerythritol tetra(3 - (3,5 - di - tert - butyl - 4 - hydroxyphenyl) propionate); the balance is made up with base oil, and the base oil is sunflower seed oil, camellia seed oil and Korean pine seed oil with a mass fraction ratio of 1:0.7:0.5.

[0068] Safety test

[0069] Taking the "Technical Specifications for Cosmetics Safety" in 2015 as the reference standard, the irritation evaluation of the bath oils in Examples 9 - 15 was carried out. The test method was the skin patch test, and 70 people randomly distributed aged 16 - 65 years were tested.

[0070] Test method: Put the test substance into the patch tester, with a dosage of 0.020 - 0.025 g. Cover the patch tester with the test substance on the back or the flexor side of the forearm of the subject with a non - irritating cloth - based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester for 30 minutes, observe the skin reaction after the indentation disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test respectively.

[0071] Evaluation criteria:

[0072] Grade 0: Negative reaction;

[0073] Grade 1: Suspected reaction, only with faint erythema;

[0074] Grade 2: Weak positive reaction, with erythema, infiltration, edema, and may have papules;

[0075] Grade 3: Strong positive reaction, with erythema, infiltration, edema, may have papules, and the reaction can exceed the test area;

[0076] Grade 4: Extremely strong positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0077] Test results: The skin reactions of all subjects were negative.

[0078] The above test results show that the bath oil provided by the present invention is mild and non - irritating to the skin and is safe to use.

[0079] Zebrafish tail moisturizing test

[0080] The zebrafish epidermis has a certain osmotic pressure tolerance range. Exceeding the tolerance range will cause water loss. Treating zebrafish with a high - osmotic pressure solution will cause its tail to shrink and become smaller due to water loss. Therefore, an induction model for zebrafish water replenishment and moisturization can be established to further evaluate the effect of moisturizing products.

[0081] (1) Fish pairing and breeding: Pair zebrafish for breeding according to a male - female ratio of 2:1, and collect fish eggs and incubate them overnight (in a 28 °C incubator).

[0082] (2) Experimental grouping: The experiment set up a blank control group and an experimental group. The experimental group included a model group, a positive group, and a sample group, with 8 2 - day - post - fertilization (dpf) larvae in each group.

[0083] (3) Preparation of solution: Accurately weigh 1 mg of the samples of Examples 9-15 and Comparative Examples 14-24, and prepare the sample solution to be tested. Use urea (0.05%) as the positive control for the experiment, and add 1 mg of the sample to each well. After the administration, place the 6-well plate in an incubator at 28°C and incubate it in the dark for 24 h.

[0084]

[0085] Test results: As shown in Table 6 below.

[0086] Table 3 Zebrafish Moisturizing Effect Test

[0087]

[0088]

[0089] According to the results analysis in Table 3: From the results of Examples 9-15, it can be seen that the compositions provided in the examples of the present invention have good efficacy, especially the effects of Examples 10-11 are better, indicating that the process and formula combination defined in the present invention can effectively improve the inhibitory effect on the reduction of zebrafish tail area.

[0090] From the results of Example 10 and Comparative Examples 17-23, the composition has a significant synergistic effect.

[0091] By comparing the results of Example 10 with those of Comparative Examples 14-16, it can be seen that the composition has a synergistic effect, and different strains or different raw materials using the same process parameters cannot achieve the inhibitory effect on the reduction of zebrafish tail area defined in the present invention.

[0092] By comparing Example 10 with Comparative Example 24, the effect of Example 10 is the best, and the process defined in the present invention can effectively improve the inhibition of the composition on the reduction of zebrafish tail area.

[0093] ABTS + Free Radical Inhibition Experiment

[0094] Take 7 mmol / L ABTS + 5 mL and 88 μL of 140 mmol / L potassium persulfate are mixed evenly to prepare the ABTS + free radical stock solution, which is stored in the dark for later use. Take 0.1 mL of the test solutions of Examples 9-15 and Comparative Examples 14-24 at 10 mg / L, add 3.9 mL of the ABTS + working solution, shake for 30 s, let it stand at room temperature for 6 min, then use absolute ethanol as the control, measure the absorbance at 734 nm, and use Vc as the positive control. Each sample is measured 3 times repeatedly, and the scavenging rate of ABTS+ free radicals is calculated according to the following formula:

[0095]

[0096] Where Ai is the absorbance value of the test solution added; Aj is the background value of the test solution; A0 is the control value without adding the test solution.

[0097] Table 4

[0098] Experimental group <![CDATA[ABTS + Free radical scavenging rate / % <!-- 8 -->]]> Example 9 79.39 Example 10 83.17 Example 11 83.42 Example 12 80.52 Example 13 81.47 Example 14 80.33 Example 15 78.49 Comparative Example 14 67.41 Comparative Example 15 65.37 Comparative Example 16 32.54 Comparative Example 17 55.93 Comparative Example 18 58.42 Comparative Example 19 64.37 Comparative Example 20 75.37 Comparative Example 21 43.51 Comparative Example 22 57.64 Comparative Example 23 48.39 Comparative Example 24 15.17 Positive control 100

[0099] Comparing the results of Examples 9-15 and Comparative Examples 14-24, it can be seen that when using the specific combination and specific ratio of the present invention and a specific extraction process, the radical scavenging rate of the composition against ABTS can be significantly improved. + radical scavenging rate.

[0100] Comparing the results of Example 10 and Comparative Examples 17-23, it can be seen that when using the specific combination and specific ratio of the present invention, the radical scavenging rate of the composition against ABTS can be significantly improved. + radical scavenging rate.

[0101] Comparing the results of Example 10 and Comparative Examples 14-16, it can be seen that when using the specific extraction process of the present invention, the radical scavenging rate of the composition against ABTS can be significantly improved. + radical scavenging rate.

[0102] Using sensory testing

[0103] Regarding the usability properties of the bath oil of the present invention, such as mildness, moisturizing property, cleaning property, and antipruritic effect, after 10 volunteers used it, the effect was evaluated. The scoring standard adopted a 10-point system, and the higher the score, the better the effect. The statistical results were taken as the average value. See Table 5 for specific test data.

[0104] Table 5

[0105]

[0106] The results show that: From the results of the examples and comparative examples, it can be obtained in terms of mildness, moisturizing property, cleaning property, and whether there is skin itching that the examples are superior to the comparative examples. Therefore, when prepared according to the ratios defined in the present invention, any omission cannot achieve the effects achieved by the present invention. And the omissions in the comparative examples also prove that there is a certain synergistic effect among the ratios defined in the present invention.

[0107] The embodiments described above are some embodiments of the present application, rather than all embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application claimed, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative efforts fall within the scope of protection of the present application.

Claims

1. An active composition, characterized in that, Comprising the following raw materials in parts by mass: PEG-5 brassinolide 0.3 - 0.6 parts; Lanolin oil 1 - 2 parts; Betula platyphylla Suk. oil 2 - 4 parts; Michelia alba DC. oil 1 - 3 parts; Macadamia ternifolia seed oil 1 - 2 parts; Orbignya phalerata Mart. oil 1 - 2 parts; Vanilla planifolia Andr. var. genuina (Wall.) Rolfe oil 0.5 - 0.9 parts; Mixed fermentation extract of Borago officinalis L. seeds and Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf seeds 0.1 - 0.5 parts; The extraction method of the said mixed fermentation extract is as follows: S1: Take dried Borago officinalis L. seeds and Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf seeds, grind and crush them, and then soak them in water for 12h - 16h; S2: Take the suspension after soaking in S1, after high-temperature sterilization, wait until it cools to room temperature, inoculate Trichoderma reesei, ferment for 3 - 4 days, the initial fermentation temperature is 28 - 30°C, inactivate and filter to obtain filtrate A; S3: Freeze-dry filtrate A to obtain the mixed fermentation extract of Borago officinalis L. seeds and Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf seeds; The mass fraction ratio of Borago officinalis L. seeds to Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf seeds is 1:2 - 3; the mass percentage of Trichoderma reesei accounts for 2 - 4% of the total mass of Borago officinalis L. seeds and Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf seeds.

2. A bath oil, characterized in that Containing 1% - 5% by mass of the composition described in claim 1.

3. The bath oil according to claim 2, wherein It also includes base oil, emulsifier, surfactant, humectant, antioxidant.

4. The bath oil according to claim 3, characterized in that, The base oil is jojoba seed oil and / or camellia seed oil and / or Korean pine seed oil and / or sunflower seed oil.

5. The bath oil according to claim 3, wherein The emulsifier is laureth-4 and / or PEG-25 hydrogenated castor oil.

6. The bath oil according to claim 3, characterized in that, The surfactant is sodium lauryl polyether sulfate MIPA salt and / or stearamidopropyl dimethylamine.

7. The bath oil according to claim 3, wherein The humectant is one or more of propylene glycol and glycerol; the antioxidant is pentaerythrityl tetrakis (bis - tert-butylhydroxyhydrocinnamate).

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