A neuroendoscopic submucosal stem cell transplantation system and device for nasal olfactory region

Abstract

The present invention provides a system and device for submucosal stem cell transplantation in the olfactory region under neuroendoscopy, and relates to the field of intelligent medical care. The system includes: a fixing module for fixing a subject in a supine position; a puncture point positioning module for positioning the middle turbinate and the corresponding nasal septum mucosal area on the opposite side of the middle turbinate under neuroendoscopy; any point in the head area of ​​the nasal septum mucosal area is used as the puncture point; a puncture module for puncturing the puncture piece along the puncture point, and after the puncture, the puncture piece continues to move forward Mcm under the mucosa pointing to the head side of the anterior wall of the sphenoid sinus; a stem cell injection module for controlling the puncture piece to inject stem cells, and after the injection, the puncture piece is withdrawn, and the transplantation is completed. The present application locates the puncture site through a unique method, and injects stem cells based on the puncture site, which can achieve a clinical effect of high transplantation efficiency and good treatment effect.

Description

Technical Field

[0001] The present invention relates to the field of intelligent medical treatment, and more specifically, to a system and device for submucosal stem cell transplantation in the nasal olfactory region under neuroendoscopy. Background Art

[0002] In recent years, stem cell therapy for neurodegenerative diseases (Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer's disease, etc.) has become a key breakthrough area for researchers. Stem cell therapy can repair damaged DA neurons, rebuild brain neural circuits, and restore dopamine secretion levels, and is expected to become a new treatment approach.

[0003] Stem cell-related adverse events are closely related to the route of administration. Although intrathecal, intravenous or arterial injection is a common route of delivery for stem cell therapy, preclinical studies have shown that the nasal mucosal route of stem cell transplantation is a promising cell delivery method for the treatment of neurological diseases, with multiple advantages such as simple operation, convenience, and non-invasiveness, which can effectively reduce the incidence of adverse reactions. Therefore, there is an urgent need for a nasal mucosal route of stem cell transplantation system with high transplantation efficiency and good therapeutic effect for the study of related diseases. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a system and device for submucosal stem cell transplantation in the olfactory region through a neuroendoscopy; the system of the present invention locates the puncture site through a unique method, and injects stem cells based on the puncture site, which can achieve a clinical effect of high transplantation efficiency and good treatment effect.

[0005] The first aspect of the present application discloses a neuroendoscopic transnasal olfactory submucosal stem cell transplantation system, the system comprising:

[0006] A fixation module, used to fix the subject in a supine position;

[0007] A puncture point positioning module is used to locate the middle turbinate and the corresponding nasal septum mucosa area on the opposite side of the middle turbinate under neuroendoscopy; any point in the head side area of ​​the nasal septum mucosa area is used as the puncture point;

[0008] A puncture module, used for puncturing the puncture member along the puncture point, and after puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus;

[0009] The stem cell injection module is used to control the puncture member to inject stem cells. After the injection, the puncture member is withdrawn and the transplantation is completed.

[0010] In some embodiments, the head side region is one third of the head side region;

[0011] Optionally, any point on the region is preferably a point on the region closest to the external nostril;

[0012] Optionally, the head side of the anterior sphenoid sinus wall is the direction of the upper half of the anterior sphenoid sinus wall.

[0013] In some embodiments, M is a positive number greater than 0; the range of M is 3-5, preferably 4.

[0014] In some embodiments, in the stem cell injection module, the injection speed of stem cells is 100-200 μL / min, preferably 150 μL / min.

[0015] In some embodiments, in the stem cell injection module, after the stem cells are injected, the puncture member remains in its original position for N minutes and then withdraws. Optionally, N is a positive number greater than 0. The range of M is 1-4, preferably 3.

[0016] In some embodiments, between the fixing module and the puncture point positioning module, the system further includes: a disinfection module for disinfecting the nasal cavity on the surgical side;

[0017] Optionally, between the disinfection module and the puncture point positioning module, the system further includes: an anesthesia module for anesthetizing the middle turbinate and nasal septum area in the nasal cavity on the surgical side;

[0018] Optionally, the fixation module fixes the subject's head in a supine position.

[0019] In some embodiments, after the stem cell injection module, the system further comprises a hemostasis module for stopping bleeding at the puncture point, and the transplantation is completed.

[0020] The second aspect of the present application discloses a neuroendoscopic transnasal olfactory submucosal stem cell transplantation device, the device comprising: a single or multiple processors and a memory; the memory is used to store a single or multiple computer programs; when the single or multiple computer programs are executed by the single or multiple processors, the following method is implemented:

[0021] 201, immobilized subjects in supine position;

[0022] 202. Locate the middle turbinate and the corresponding nasal septum mucosa region on the opposite side of the middle turbinate under neuroendoscopy; any point in the head side region of the nasal septum mucosa region is used as the puncture point;

[0023] 203, puncture the puncture member along the puncture point, and after puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus;

[0024] 204, controlling the puncture member to inject stem cells, and withdrawing the puncture member after the injection, and the transplantation is completed.

[0025] In some embodiments, between 201 and 202, the method further includes:

[0026] 2011, disinfect the nasal cavity on the surgical side;

[0027] 2012. Anesthetize the middle turbinate and nasal septum areas in the nasal cavity on the surgical side.

[0028] In some embodiments, the device further comprises: a neuroendoscope, a fixing device for fixing the subject, stem cells, and a hemostatic device;

[0029] Optionally, the fixing device for fixing the subject includes any one or more of the following: a fixing device for fixing the subject's body, a fixing device for fixing the subject's head;

[0030] Optionally, the fixing member includes any one or more of the following: a fixing frame, a fixing pillow, a fixing clip;

[0031] Optionally, the hemostatic element comprises the following: gelatin sponge;

[0032] Optionally, the puncture member includes any one or more of the following: a puncture needle, a puncture cylinder, a puncture tube, and a puncture rod.

[0033] This application has the following beneficial effects:

[0034] 1. This application innovatively discloses a neuroendoscopic transnasal olfactory submucosal stem cell transplantation system, which locates the puncture point through a three-step method, first by roughly locating the middle turbinate and the corresponding nasal septum mucosal area on the opposite side of the middle turbinate (this area is the olfactory mucosal area), and then further finely locating the head area in the olfactory mucosal area (the upper third of the head), and finally locating the arc in the head area, and selecting any point from the arc as the puncture point, preferably selecting the point closest to the external nostril in the arc as the puncture point; further, based on the puncture point, along a fixed angle (the target area where the stem cells in the head of the anterior wall of the sphenoid sinus are ultimately to be), a fixed distance is advanced, and after reaching the appropriate position, the stem cell injection operation is performed to complete the transplantation operation. This transplantation system not only has high transplantation efficiency and good therapeutic effect, but also contributes to the treatment research related to neurodegenerative diseases; and the system is simple, convenient, non-invasive, and highly repeatable, which significantly improves the patient's comfort during and after the operation.

[0035] 2. After the stem cells were transplanted using the neuroendoscopic transnasal olfactory submucosal stem cell transplantation system disclosed in the present application, the results of evaluating the subjects using a neurological function scoring scale showed that the treatment effect was better, the subject response rate was improved, and the range of motion of the upper and lower limbs was significantly increased.

[0036] 3. The present application innovatively discloses a neuroendoscopic transnasal olfactory submucosal stem cell transplantation device, which can implement the steps of the above-mentioned transplantation method to form a clinical "automatic processing station" executed by the machine end, thereby ensuring the standardization and automation of the transplantation process; at the same time, the present device also discloses specific components required for the implementation of the operation, such as the neuroendoscope, the fixing parts for fixing the subject, stem cells, hemostatic parts, etc., to ensure the smooth progress of the machine end. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0038] Figure 1 is a flowchart of a transplantation system provided by the first aspect of an embodiment of the present invention;

[0039] Figure 2 is a schematic diagram showing the sphenoid sinus opening and the middle turbinate region provided by an embodiment of the present invention;

[0040] Figure 3 is a schematic diagram of performing puncture based on puncture points provided by an embodiment of the present invention;

[0041] 1. Superior turbinate; 2. Middle turbinate; 3. Sphenoid sinus opening; 4. Olfactory area; 5. Puncture device. DETAILED DESCRIPTION

[0042] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0043] In some of the processes described in the specification and claims of the present invention and the above-mentioned figures, multiple operations that appear in a specific order are included, but it should be clearly understood that these operations may not be executed in the order in which they appear in this article or executed in parallel. The serial numbers of the operations, such as 101, 102, etc., are only used to distinguish different operations, and the serial numbers themselves do not represent any execution order. In addition, these processes may include more or fewer operations, and these operations may be executed in sequence or in parallel. It should be noted that the descriptions of "first", "second", etc. in this article are used to distinguish different messages, devices, modules, etc., do not represent the order of precedence, and do not limit the "first" and "second" to be different types.

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0045] Figure 1 The present invention provides a schematic flow chart of a neuroendoscopic transnasal olfactory submucosal stem cell transplantation system. Specifically, the system includes the following:

[0046] 101: fixation module, used to fix the subject in supine position;

[0047] In some embodiments, the term "subject" or "test subject" or "test sample" used herein refers to any animal (e.g., mammal), including but not limited to humans, non-human primates, rodents, etc., which will be the recipient of a specific treatment. Generally, the terms "subject" and "patient" are used interchangeably herein when referring to human subjects. Preferably, the subject is a human.

[0048] In some embodiments, the sample to be tested is a patient undergoing clinical prognosis assessment.

[0049] In some embodiments, between the fixing module and the puncture point positioning module, the system further includes: a disinfection module for disinfecting the nasal cavity on the surgical side, specifically, using complex iodine for disinfection;

[0050] Optionally, between the disinfection module and the puncture point positioning module, the system further includes: an anesthesia module, used to anesthetize the middle turbinate and nasal septum area in the nasal cavity on the surgical side, specifically, placing a cotton pad containing lidocaine and ropivacaine between the right middle turbinate and nasal septum for 2 minutes for anesthesia;

[0051] Optionally, the fixation module fixes the subject's head in a supine position.

[0052] 102: a puncture point positioning module, used for locating the middle turbinate and the corresponding nasal septum mucosa area on the opposite side of the middle turbinate under neuroendoscopy; any point in the head side area of ​​the nasal septum mucosa area is used as the puncture point;

[0053] In some embodiments, the head side region is one third of the head side region;

[0054] Optionally, any point on the region is preferably a point on the region closest to the external nostril;

[0055] 103: a puncture module, used for puncturing the puncture member along the puncture point, and after the puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus;

[0056] In some embodiments, the cephalad side of the anterior sphenoid sinus wall is the direction of the upper half of the anterior sphenoid sinus wall.

[0057] In some embodiments, M is a positive number greater than 0; the range of M is 3-5, preferably 4. M is determined based on the distance from the puncture point to the target point in the area where the cell is to be located;

[0058] 104: A stem cell injection module, used to control the puncture member to inject stem cells, and to withdraw the puncture member after the injection, and the transplantation is completed.

[0059] In some embodiments, in the stem cell injection module, the injection speed of stem cells is 100-200 μL / min, preferably 150 μL / min.

[0060] In some embodiments, in the stem cell injection module, after the stem cells are injected, the puncture member remains in its original position for N minutes and then withdraws. Optionally, N is a positive number greater than 0. The range of M is 1-4, preferably 3.

[0061] In some embodiments, after the stem cell injection module, the system further comprises a hemostasis module for stopping bleeding at the puncture point, and the transplantation is completed.

[0062] The transplantation system disclosed in the present application can effectively enable stem cells to bypass the blood-brain barrier and quickly enter the central nervous system. The nasal mucosa has a large area and is rich in submucosal blood vessels. Cells can pass through the blood-brain barrier and reach the brain via the olfactory nerve, trigeminal nerve and cerebrospinal fluid pathways.

[0063] The second aspect of the present application discloses a neuroendoscopic transnasal olfactory submucosal stem cell transplantation device, the device comprising: a single or multiple processors and a memory; the memory is used to store a single or multiple computer programs; when the single or multiple computer programs are executed by the single or multiple processors, the following method is implemented:

[0064] 201 fix the subject in a supine position using a processor by retrieving a computer program in a memory;

[0065] Running the computer program to cause the operating processor or processing device to perform 202 positioning the middle turbinate and the corresponding nasal septum mucosa region on the opposite side of the middle turbinate under neuroendoscopy; any point in the head side region of the nasal septum mucosa region is used as a puncture point;

[0066] Running the computer program to control the processor or processing device to select the point closest to the external nostril in the area as the puncture point;

[0067] Running the computer program to control the processor or the processing device to perform 203 puncturing the puncture member along the puncture point, and after the puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus;

[0068] The computer program is run to control the processor or the processing device to perform 204 to control the puncture member to inject stem cells. After the injection, the puncture member is operated and the transplantation is completed.

[0069] In some embodiments, between 201 and 202, the method further includes:

[0070] Running the computer program to cause the operating processor or processing device to perform the operation of disinfecting the nasal cavity on the surgical side;

[0071] The computer program is run to enable the operating processor or processing device to perform an operation of anesthetizing the middle turbinate and nasal septum areas in the nasal cavity on the surgical side.

[0072] In some embodiments, the device further comprises: a neuroendoscope, a fixing device for fixing the subject, stem cells, and a hemostatic device;

[0073] Optionally, the fixing device for fixing the subject includes any one or more of the following: a fixing device for fixing the subject's body, a fixing device for fixing the subject's head;

[0074] Optionally, the fixing member includes any one or more of the following: a fixing frame, a fixing pillow, a fixing clip;

[0075] Optionally, the hemostatic element comprises the following: gelatin sponge;

[0076] Optionally, the puncture member includes any one or more of the following: a puncture needle, a puncture cylinder, a puncture tube, and a puncture rod.

[0077] Compared with the reported operation method of inserting a hose into the nasal cavity and directly dripping stem cells into the nasal cavity, the differences and advantages of the technical solution disclosed in this application in terms of operation are summarized as follows:

[0078]

[0079]

[0080] In terms of therapeutic effect, the advantages of the technical solution disclosed in this application are summarized as follows:

[0081] In some embodiments, for patients with cerebral infarction, there is currently no effective treatment for patients with cerebral infarction. We have used the system disclosed in this application to complete neural stem cell transplantation treatment for 22 patients with cerebral infarction, and the patients' neurological functions have improved to varying degrees.

[0082] The total score of the neurological function rating scale FM-UE gradually increased over time (the therapeutic effect improved), and there were significant statistical differences between the 1-month follow-up and the 3-month follow-up compared with before transplantation (P less than 0.05). Within 1 month after transplantation, the patients obtained a median FM-UE score change of 5 points (△FM-UEmedian); within 1-3 months after transplantation, the patients obtained a median FM-UE score change of 1 point;

[0083] It can be found that the speed of improvement of upper limb function is gradually slowing down over time, but the patients are still improving as a whole (i.e., △FM-UEmedian>0), and the improvement values ​​are all statistically significant (P less than 0.05). According to the CID of △FM-UE: 4.25-7.25, the patient response rate at each follow-up time point was compared at three levels (△FM-UE≥5,≥6,≥7): regardless of the CID, the response rate at 3 months after transplantation was higher than the response rate at 1 month after transplantation.

[0084] The changes in the NIHSS scores of the patients were observed. Patients with NIHSS=0 had already developed NIHSS at the 1-month follow-up, and patients with NIHSS=6 had cleared NIHSS at the 3-month follow-up. The median NIHSS change within 1 month after transplantation was -2, which was statistically significant.

[0085] The changes in the mRS scores of the patients' neurological function rating scale were observed. The number of patients with mRS=4 (moderate to severe disability) gradually decreased with the follow-up time; the proportion of patients with mRS=0, 1 or 2 (achieving functional independence) gradually increased with the follow-up time, with the proportion being 26.32% at the 1-month follow-up and 31.58% at the 3-month follow-up.

[0086] In the upper limb range of motion assessment, a total of 4 movements had significant changes before and after the follow-up, and the range of motion was increased compared with before the transplantation, namely: shoulder flexion, shoulder extension, shoulder abduction, and elbow flexion and extension. In the lower limb range of motion assessment, a total of 4 movements had significant changes before and after the follow-up, and the range of motion was increased compared with before the transplantation, namely: hip flexion, hip extension, hip abduction, and knee flexion and extension.

[0087] Specifically, among the 22 patients, 6 patients achieved a 5-point FM-UE score change 1 month after neural stem cell transplantation using this method compared with before transplantation, and 11 patients achieved a 5-point or higher FM-UE score change. 4 patients achieved a 1-point FM-UE score change 3 months after transplantation compared with 1 month after transplantation, and 11 patients achieved a 1-point or higher FM-UE score change.

[0088] Control group: 10 patients, using existing treatment methods, no changes at 1 month and 3 months.

[0089] From the above data, it can be seen that compared with existing treatment methods, the neural stem cell transplantation involved in this method has better treatment and recovery effects on patients 1 month and 3 months after surgery.

[0090] In certain specific embodiments of the device for submucosal stem cell transplantation in the olfactory region under neuroendoscopy, the processor may be a processing device, including one or more general processing devices such as a microprocessor, a central processing unit (CPU), a graphics processing unit (GPU), an accelerated processing unit (APU), etc. The processor may generate intermediate data using a software program, and may generate an intermediate 2D image or 3D image using a software program, and then the intermediate 2D image or 3D image may be subsequently stored in a memory. In certain specific embodiments, the processor may also extract a stored computer program from the memory for implementation, and multiple processors may work individually or in combination.

[0091] In certain embodiments, the computer program includes a computer execution program, a computer positioning program, and a computer judgment program. The computer execution program can execute fixed actions of the target processor or processing device in sequence. The computer positioning program can scan the target area according to a fixed image or feature and determine the target position. The computer judgment program can appear in the computer execution program or the computer positioning program to assist in executing commands or positioning commands. The computer judgment program can also be used as an independent unit to judge the information collected by position sensors, pressure sensors, photoreceptors, pH sensors, etc. and output signals to command the computer program whether to proceed to the next small module program.

[0092] In certain specific embodiments, the memory includes read-only memory (ROM), phase-change random access memory (PRAM), static random access memory (SRAM), flash memory, random access memory (RAM), dynamic random access memory (DRAM) such as synchronous DRAM (SDRAM), electrically erasable programmable read-only memory (EEPROM), static memory (e.g., flash memory, flash disk, static random access memory), and other types of random access memory, cache memory, registers, compact disk read-only memory (CD-ROM), digital versatile disk (DVD) or other optical storage device, cassette, other magnetic storage device, or any other non-transitory medium that can be used to store information (e.g., stored in any format) including images, data, or computer executable instructions that can be accessed by a processor or any other type of computer device. Computer program instructions can be accessed by a processor, read from ROM or any other suitable memory location and loaded into RAM for execution by the processor. For example, the memory can store one or more software applications. The software applications stored in the memory can include, for example, operating systems for common computer systems and for devices controlled by software.

[0093] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0094] In the several embodiments provided in the present application, it should be understood that the disclosed systems, devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interfaces, devices or units, which can be electrical, mechanical or other forms.

[0095] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0096] In addition, each functional unit in each embodiment of the present invention may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.

[0097] The exemplary embodiments of the present disclosure described in detail above are merely illustrative and not restrictive. It should be understood by those skilled in the art that various modifications and combinations may be made to these embodiments or their features without departing from the principles and spirit of the present disclosure, and such modifications should fall within the scope of the present disclosure.

Claims

1. A neuroendoscopic transnasal olfactory submucosal stem cell transplantation system, characterized in that: The system comprises: A fixation module, used to fix the subject in a supine position; The puncture point positioning module is used to locate the middle turbinate and the corresponding nasal septum mucosa area on the opposite side of the middle turbinate as the olfactory mucosa area under neuroendoscopy; finely locate the upper third of the head side of the olfactory mucosa area; locate the arc of the upper third of the head side, and select the point closest to the external nostril from the arc as the puncture point; A puncture module, used for puncturing the puncture member along the puncture point, and after puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus; The stem cell injection module is used to control the puncture member to inject stem cells. After the injection, the puncture member is withdrawn and the transplantation is completed.

2. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: The head side of the anterior sphenoid sinus wall is the direction of the upper half of the anterior sphenoid sinus wall.

3. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: The M is a positive number greater than 0; the range of M is 3-5.

4. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 3, characterized in that: The M is 4.

5. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: In the stem cell injection module, the speed of injecting stem cells is 100-200 μl / min.

6. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 5, characterized in that: The rate of stem cell injection was 150 μl / min.

7. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: In the stem cell injection module, after the stem cells are injected, the puncture member remains in the original position for N min and then withdraws.

8. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 7, characterized in that: The N is a positive number greater than 0; the range of N is 1-4.

9. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 8, characterized in that: The N is 3.

10. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: Between the fixing module and the puncture point positioning module, the system further includes: a disinfection module for disinfecting the nasal cavity on the surgical side.

11. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 10, characterized in that: Between the disinfection module and the puncture point positioning module, the system further includes: an anesthesia module for anesthetizing the middle turbinate and nasal septum area in the nasal cavity on the surgical side.

12. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: The fixing module fixes the subject's head in a supine position.

13. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation system according to claim 1, characterized in that: The system further comprises a hemostasis module for hemostasis of the puncture point.

14. A neuroendoscopic transnasal olfactory submucosal stem cell transplantation device, characterized in that: The device comprises: a single or multiple processors and a memory; the memory is used to store a single or multiple computer programs; when the single or multiple computer programs are executed by the single or multiple processors, the following method is implemented: 201, immobilized subjects in supine position; 202. Locate the middle turbinate and the corresponding nasal septum mucosa area on the opposite side of the middle turbinate as the olfactory mucosa area under neuroendoscopy; carefully locate the upper third of the head side of the olfactory mucosa area; locate the arc of the upper third of the head side, and select the point closest to the external nostril from the arc as the puncture point; 203, puncture the puncture member along the puncture point, and after puncture, the puncture member continues to move forward Mcm under the mucosa toward the head side of the anterior wall of the sphenoid sinus; 204, controlling the puncture member to inject stem cells, and withdrawing the puncture member after the injection, and the transplantation is completed.

15. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 14, characterized in that: Between 201 and 202, the methods implemented also include: 2011, disinfect the nasal cavity on the surgical side; 2012. Anesthetize the middle turbinate and nasal septum areas in the nasal cavity on the surgical side.

16. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 14, characterized in that: The device also includes: a neuroendoscope, a fixing device for fixing the subject, stem cells, and a hemostatic device.

17. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 16, characterized in that: The fixing device for fixing the subject includes any one or more of the following: a fixing device for fixing the subject's body, and a fixing device for fixing the subject's head.

18. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 17, characterized in that: The fixing member includes any one or more of the following: a fixing frame, a fixing pillow, and a fixing clip.

19. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 16, characterized in that: The hemostatic device includes the following: gelatin sponge.

20. The neuroendoscopic transnasal olfactory submucosal stem cell transplantation device according to claim 14, characterized in that: The puncture member includes any one or more of the following: a puncture needle, a puncture cylinder, a puncture tube, and a puncture rod.

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