Composition for improving skin aging, preparation method and use thereof

Abstract

The present invention belongs to the field of cosmetic technology, and specifically relates to a composition for improving skin aging, a preparation method thereof and a use thereof. The present invention provides a composition for improving skin aging, which is an oil-soluble package made of superoxide dismutase, carboxymethyl chitosan and yeast extract as core raw materials, and is matched with 1,2-pentanediol, jojoba seed oil, caprylic / capric triglyceride and an emulsifier. The composition for improving skin aging has the advantages of high stability and good skin transdermal permeability, and can effectively ensure the anti-aging effect of the composition. At the same time, the composition for improving skin aging also has significant antioxidant capacity and the effect of enhancing skin barrier function, which can fundamentally delay the process of skin aging.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition for improving skin aging, a preparation method and use thereof. Background Art

[0002] Skin aging is a long and complex evolutionary process. With the in-depth study of the mechanism of skin aging, there are many theories about the mechanism of skin aging, such as: the theory of gene regulation of skin aging, the theory of free radicals of skin aging, the theory of metabolic disorders of skin aging, and the photostimulation of skin aging. Among them, genetic changes are the root of skin aging, free radical damage to cells is the cause of skin aging, metabolic disorders of the body are the basis of skin aging, and the external environment, such as light stimulation, air pollution and other harmful factors, accelerate the process of skin aging.

[0003] Skin is the largest organ in the human body. If it is affected by internal factors and external factors in the environment for a long time, the functional integrity of the epidermis and dermis will be damaged, and symptoms such as dryness, sagging, dullness and deepening of wrinkles will gradually appear. These symptoms have attracted more and more attention. At present, anti-aging products have become the most popular products among consumers, especially anti-aging cosmetics are the most popular products among female consumers.

[0004] Patent document CN107411997A discloses an anti-aging cream composition. The anti-aging cream contains nonapeptide-1, tripeptide-1 copper, acetyl octapeptide-3 and palmitoyl tripeptide-5. The composition can improve dull yellow skin color and the first appearance of fine lines on the skin, and can also reduce and alleviate persistent and deep smile lines, dry lines, nasolabial folds, etc., and has good anti-aging effects.

[0005] Patent document CN109288770A discloses an anti-aging composition. The anti-aging composition contains glyceryl glucoside, sodium hyaluronate, barley extract, and ascorbic acid polypeptide. The components of the composition can be directly engulfed by skin cells to regenerate epidermal and dermal cells, accelerate metabolism, and effectively reduce fine lines, wrinkles and skin roughness, which can resist aging more comprehensively and make the product have better anti-aging effect.

[0006] Patent document CN117064065A discloses an anti-aging composition and its application. The anti-aging composition contains collagen peptides, hyaluronic acid or its salt and zinc as main active ingredients. Collagen peptides, hyaluronic acid or its salt and zinc can interact with each other to promote cell growth, division, proliferation and differentiation. While maintaining their respective effects, they also play a synergistic role to enhance human protein synthesis, making the skin smooth, delicate, shiny and no longer rough, and having good anti-aging effects.

[0007] However, the anti-aging cosmetics on the market currently mainly use peptides as the main ingredients for wrinkle removal and anti-aging. The stability of such peptide cosmetics is poor during the shelf life, and the active degradation of the peptide active ingredients is prone to occur, resulting in the defect that the wrinkle removal and anti-aging effects are not lasting. Therefore, researching and developing anti-aging cosmetics with obvious anti-aging effects, lasting effects, and high stability is still a problem that needs to be solved urgently. Summary of the invention

[0008] In order to solve the defects of the prior art, the present invention provides a composition for improving skin aging, and a preparation method and use thereof. The present invention provides an oil-soluble encapsulated composition for improving skin aging, which is made of superoxide dismutase, carboxymethyl chitosan and yeast extract as core raw materials, and is matched with 1,2-pentanediol, jojoba seed oil, caprylic / capric triglyceride and an emulsifier. The components in the composition cooperate with each other to achieve the effects of whitening, moisturizing, wrinkle removal and anti-aging.

[0009] The present invention provides a composition for improving skin aging, comprising the following components and their mass percentages:

[0010] Superoxide dismutase 0.5-1.5%, carboxymethyl chitosan 1-2%, yeast extract 2-8%, emulsifier 18-25%, 1,2-pentanediol 10-15%, jojoba seed oil 10-15%, caprylic / capric triglyceride 35-45%, purified water added to 100%.

[0011] Furthermore, the mass ratio of the superoxide dismutase to carboxymethyl chitosan is 1:(1.2-2.8).

[0012] Furthermore, the mass ratio of the superoxide dismutase to carboxymethyl chitosan is 1:2.

[0013] Furthermore, the mass ratio of superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:(1.2-2.8):(2-5).

[0014] Furthermore, the mass ratio of superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:4.

[0015] Furthermore, the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of (1-2):(3-6).

[0016] Furthermore, the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:5.

[0017] The present invention also provides a method for preparing the composition for improving skin aging, comprising the following steps:

[0018] Step S1, adding yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol into purified water, and stirring evenly at 350-450 rpm to obtain an aqueous phase;

[0019] Step S2, mixing the emulsifier, jojoba seed oil and caprylic / capric triglyceride, and stirring at 500-600 rpm for 10-15 min to obtain an oil phase;

[0020] Step S3, under stirring, add the water phase obtained in step S1 to the oil phase obtained in step S2, and then stir at 400-500 rpm for 20-25 min to obtain the product.

[0021] In addition, the present invention also provides the use of the composition for improving skin aging in the preparation of anti-aging cosmetics. The cosmetics include BB cream, lotion, essence oil, face cream and other full-oil formula beauty cosmetics.

[0022] As the inventors further studied the mechanism of skin aging, they found that enhancing skin barrier function and improving skin antioxidant capacity are important ways to delay skin aging. Superoxide dismutase (SOD) is a class of enzymes that can catalyze superoxide anion free radicals (O 2 - ) is disproportionated to H 2 O 2 and O 2 The enzyme is a natural free radical scavenger in the body. However, natural SOD has the defects of poor stability and low activity, which seriously affects the application of SOD in cosmetics.

[0023] In order to improve the stability of SOD, the inventor consulted a large number of literature and materials, and for the first time proposed the combined use of SOD and carboxymethyl chitosan in the study of anti-aging cosmetics. After a lot of trial and error, the inventor found that the use of a specific ratio of carboxymethyl chitosan and SOD can not only improve the stability of SOD, but also improve the enzyme activity of SOD, thereby solving the defects of poor stability and low activity of SOD during use.

[0024] At the same time, the inventors also found that the mixed use of carboxymethyl chitosan and SOD in a specific ratio can synergistically enhance the effect of scavenging active oxygen free radicals. Although SOD can scavenge superoxide anion free radicals, it does not have a direct scavenging effect on hydroxyl free radicals, while carboxymethyl chitosan has a strong scavenging ability for hydroxyl free radicals and DPPH free radicals. The combined use of the two in a specific ratio can complementarily enhance the scavenging effect of active oxygen free radicals, reduce the damage of active oxygen free radicals to the skin, and thus slow down the process of skin aging.

[0025] In addition, the inventors found in in-depth research that the activity of catalase has a huge impact on the scavenging effect of reactive oxygen free radicals. When SOD dismutates superoxide anion radicals into hydrogen peroxide, it needs to be acted upon by catalase to convert hydrogen peroxide into water and oxygen. If the activity of catalase is reduced, the content of hydrogen peroxide in the body will increase, thereby increasing the content of hydroxyl free radicals, and the effect of scavenging excess reactive oxygen free radicals cannot be achieved. In response to this, the inventors proposed the idea of ​​mixing yeast extract with SOD and carboxymethyl chitosan. During the experiment, it was unexpectedly discovered that when SOD, carboxymethyl chitosan and yeast extract were mixed in a specific ratio, the scavenging effect on reactive oxygen free radicals was far better than when SOD and carboxymethyl chitosan were mixed. The inventors speculate that the reason is that the components with strong nucleophilic properties in the yeast extract can stimulate and enhance the activity of catalase. When SOD, carboxymethyl deacetylated chitosan and yeast extract are mixed in a specific ratio, the interaction between the three can accelerate the scavenging effect of catalase on hydrogen peroxide, hydroxyl radicals and superoxide anion radicals, thereby improving the antioxidant capacity of the composition for improving skin aging.

[0026] In addition, the composition for improving skin aging provided by the present invention also has the effect of enhancing the skin barrier function and promoting the production of skin collagen. The inventors have found that yeast extract can not only allow the skin to easily absorb nutrients, but also help to moisturize and repair the skin barrier, and promote the metabolism of keratinocytes; while carboxymethyl deacetylated chitosan can accelerate the regeneration rate of epidermal cells, and the two can synergistically enhance the barrier function of the skin and improve the barrier ability of the skin to the external environment. At the same time, the inventors also found that yeast extract can not only promote the proliferation of fibroblasts, but also provide the required amino acid raw materials for the synthesis of collagen, while carboxymethyl deacetylated chitosan can stimulate fibroblasts to synthesize collagen, and the two can synergistically increase the content of collagen in the skin quickly, and finally SOD is used to stabilize the collagen and elastin in the skin. The synergistic effect of the three can effectively maintain the content of collagen in the skin, keep the skin firm and elastic, and can fundamentally delay the problem of skin aging.

[0027] Furthermore, in order to better solve the problem of skin absorption of cosmetics, the inventors used an oil-soluble encapsulation system to encapsulate the core ingredients, which can not only effectively improve the transdermal absorption rate of SOD and carboxymethyl deacetylated chitosan, but also further improve the stability of the composition, thereby ensuring the anti-aging effect of the composition.

[0028] Compared with the prior art, the composition for improving skin aging provided by the present invention has the following advantages:

[0029] (1) The composition for improving skin aging provided by the present invention has the advantages of high stability and good skin transdermal permeability, which can effectively ensure the anti-aging effect of the composition.

[0030] (2) The composition for improving skin aging provided by the present invention can not only improve the skin's antioxidant capacity, but also effectively enhance the skin's barrier function, thereby solving the aging problems of skin collagen loss, decreased elasticity, and dull skin tone from both internal and external aspects, and can fundamentally delay the process of skin aging. DETAILED DESCRIPTION

[0031] The present invention is further described below through the description of specific implementation methods, but this is not a limitation of the present invention. Those skilled in the art can make various modifications or improvements based on the basic concept of the present invention, but as long as they do not deviate from the basic concept of the present invention, they are all within the scope of the present invention. The raw materials used in the present invention are all conventional cosmetic raw materials and commercially available products. Among them: the English name of the superoxide dismutase is: SUPEROXIDE DISMUTASE, CAS No.: 9054-89-1; the English name of the carboxymethyl deacetylated chitosan is: CARBOXYMETHYL CHITOSAN, the molecular formula is: C 20 H 37 N 3 O 14 , CAS number is: 83512-85-0; the English name of the yeast extract is: YEAST EXTRACT, CAS number is: 8013-01-2.

[0032] Example 1: A composition for improving skin aging

[0033] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0034] 1.5% superoxide dismutase, 1.8% carboxymethyl chitosan, 4.5% yeast extract, 18% emulsifier, 15% 1,2-pentanediol, 15% jojoba seed oil, 38% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:1.2:3; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:3.

[0035] Preparation method:

[0036] Step S1, adding yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol into purified water, and stirring evenly at 350 rpm to obtain an aqueous phase;

[0037] Step S2, mixing the emulsifier, jojoba seed oil and caprylic / capric triglyceride, and stirring at 500 rpm for 15 min to obtain an oil phase;

[0038] Step S3, under stirring, add the water phase obtained in step S1 to the oil phase obtained in step S2, and then stir at 400 rpm for 25 minutes to obtain.

[0039] Example 2: A composition for improving skin aging

[0040] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0041] 1% superoxide dismutase, 2% carboxymethyl chitosan, 4% yeast extract, 20% emulsifier, 12% 1,2-pentanediol, 14% jojoba seed oil, 40% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:4; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:5.

[0042] Preparation method:

[0043] Step S1, adding yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol into purified water, and stirring evenly at 400 rpm to obtain an aqueous phase;

[0044] Step S2, mixing the emulsifier, jojoba seed oil and caprylic / capric triglyceride, and stirring at 550 rpm for 12 min to obtain an oil phase;

[0045] Step S3, under stirring, add the water phase obtained in step S1 to the oil phase obtained in step S2, and then stir at 450 rpm for 22 minutes to obtain.

[0046] Example 3: A composition for improving skin aging

[0047] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0048] 0.5% superoxide dismutase, 1.4% carboxymethyl chitosan, 2.5% yeast extract, 25% emulsifier, 10% 1,2-pentanediol, 12% jojoba seed oil, 42% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2.8:5; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:4.

[0049] Preparation method:

[0050] Step S1, adding yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol into purified water, and stirring evenly at 450 rpm to obtain an aqueous phase;

[0051] Step S2, mixing the emulsifier, jojoba seed oil and caprylic / capric triglyceride, and stirring at 600 rpm for 10 min to obtain an oil phase;

[0052] Step S3, under stirring, add the water phase obtained in step S1 to the oil phase obtained in step S2, and then stir at 500 rpm for 20 minutes to obtain.

[0053] Comparative Example 1: A composition for improving skin aging

[0054] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0055] 1% superoxide dismutase, 2% trehalose, 4% yeast extract, 20% emulsifier, 12% 1,2-pentanediol, 14% jojoba seed oil, 40% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:4; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:5.

[0056] The preparation method is similar to that of Example 2.

[0057] The difference from Example 2 is that carboxymethyl chitosan is replaced by trehalose.

[0058] Comparative Example 2: A composition for improving skin aging

[0059] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0060] 1% superoxide dismutase, 2% carboxymethyl chitosan, 4% glutathione, 20% emulsifier, 12% 1,2-pentanediol, 14% jojoba seed oil, 40% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:4; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:5.

[0061] The preparation method is similar to that of Example 2.

[0062] The difference from Example 2 is that yeast extract is replaced by glutathione.

[0063] Comparative Example 3: A composition for improving skin aging

[0064] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0065] 1.5% superoxide dismutase, 1.5% carboxymethyl chitosan, 1.5% yeast extract, 20% emulsifier, 12% 1,2-pentanediol, 14% jojoba seed oil, 40% caprylic / capric triglyceride, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:1:1; the emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:5.

[0066] The preparation method is similar to that of Example 2.

[0067] The difference from Example 2 is that the mass ratio of superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:1:1.

[0068] Comparative Example 4: A composition for improving skin aging

[0069] The composition for improving skin aging is composed of the following components in terms of mass percentage:

[0070] 1% superoxide dismutase, 2% carboxymethyl chitosan, 4% yeast extract, 12% 1,2-pentanediol, and purified water added to 100%; the mass ratio of the superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:4.

[0071] Preparation method:

[0072] Yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol are added into purified water, and stirred evenly at 400 rpm to obtain the product.

[0073] Test Example 1: Test for determination of SOD activity in a composition for improving skin aging

[0074] 1. Test materials:

[0075] The compositions for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 3 and Comparative Example 4.

[0076] 2. Test methods:

[0077] The activity of SOD in the composition for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 3 and Comparative Example 4 was measured. The determination method was determined by referring to the method for self-oxidation of pyrogallol in GB / T5009.171-2003. 10 mL of each sample of the composition for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 3 and Comparative Example 4 was taken, placed in a sealed vial in a 40°C constant temperature heating box, and samples were taken regularly (5 days, 10 days, 15 days, 30 days, 60 days) for self-oxidation rate determination.

[0078] Among them: The activity calculation formula of SOD is as follows:

[0079]

[0080] Where: △A325 is the reaction rate of the self-oxidation tube;

[0081] △A′325 is the reaction rate of the sample tube;

[0082] D is the dilution factor of the test sample.

[0083] Vt is the total volume of the reaction solution, in ml;

[0084] V is the volume of the test sample added, in ml;

[0085] 3. Test results:

[0086] The test results are shown in Table 1.

[0087] Table 1 Determination results of SOD activity in the composition for improving skin aging

[0088] 5 days 10 days 15 days 30 days 60 days Example 1 5.24％ 4.16％ 3.57％ 3.17％ 2.19％ Example 2 5.86％ 5.25％ 4.62％ 3.83％ 3.21％ Example 3 5.17％ 4.09％ 3.43％ 2.89％ 2.08％ Comparative Example 1 0.80％ -2.54％ -5.63％ -8.26％ -18.33％ Comparative Example 3 1.83％ -1.26％ -3.13％ -5.08％ -7.64％ Comparative Example 4 1.34％ -1.65％ -3.37％ -5.76％ -8.12％

[0089] As can be seen from Table 1, the SOD activity in the composition for improving skin aging prepared in Examples 1 to 3 of the present invention has a high stability under a high temperature condition of 40° C. However, the SOD activity in the composition for improving skin aging prepared in Comparative Examples 1, 3, and 4 decreased significantly, indicating that the components in the composition for improving skin aging provided by the present invention act synergistically to improve the stability and activity of SOD, and can ensure the efficacy of SOD in the composition for improving skin aging.

[0090] Test Example 2: Antioxidant Determination Test of Composition for Improving Skin Aging

[0091] 1. Test materials:

[0092] The compositions for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4.

[0093] 2. Test methods:

[0094] The superoxide anion free radicals ((O 2 - ·) clearance rate and hydroxyl radical (·OH) clearance rate.

[0095] 2.1. Determination of superoxide anion free radical scavenging rate:

[0096] The scavenging rate of superoxide anions was determined by the pyrogallol method. The final concentration of pyrogallol was 0.1 mmol / L. 100 μL of the composition for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 was added to a constant temperature water bath at 25° C., and double distilled water was used as a control. A was measured at 320 nm at 4 min. 320 The background setting uses double distilled water instead of pyrogallol. The superoxide anion radical scavenging rate is calculated as follows:

[0097] Clearance S% = [A 空 -(A 样 -A 底 )] / A 空 ×100%

[0098] Among them: A 空 is the absorbance value of the blank tube;

[0099] A 样 Indicates the absorbance value of the sample tube;

[0100] A 底 Indicates the background absorbance value.

[0101] 2.2 Determination of hydroxyl radical scavenging rate:

[0102] The content of hydroxyl free radicals was indirectly determined by measuring the change in absorbance of hydroxyl free radicals generated by Fenton reaction after reacting with a specific color developer using spectrophotometry. Take 10 mL of the composition for improving skin aging prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4, add a Tris-HCl solution with a pH of 4.5 into a volumetric flask, and dilute to 25 mL with double distilled water, and measure the absorbance of the following solutions: (1) 5 mL of methyl violet solution (2.0×10 -3 mol / L), and its absorbance is measured as A; (2) 5 mL of methyl violet solution (2.0×10 -3 mol / L)、2.5mLFe 2+ Solution (1.0×10-3 mol / L)、1.25mLH 2 O 2 Solution (0.12%), measured its absorbance as A 0 ; (3) 2.5 mL of methyl violet solution (2.0 × 10 -3 mol / L)、2.5mLFe 2+ Solution (1.0×10 -3 mol / L)、1.25mLH 2 O 2 solution (0.12%), 10 mL sample solution, and its absorbance was measured as As.

[0103] The hydroxyl radical scavenging rate was calculated as follows:

[0104] Clearance S% = [(A s -A 0 ) / (AA 0 )]×100%

[0105] 3. Test results:

[0106] The test results are shown in Tables 2 and 3.

[0107] Table 2 Antioxidant test results of the composition for improving skin aging on day 0

[0108] Group Superoxide anion free radical scavenging rate (%) Hydroxyl free radical scavenging rate (%) Example 1 81.6 95.8 Example 2 85.9 98.2 Example 3 80.4 93.5 Comparative Example 1 42.8 55.4 Comparative Example 2 68.5 73.6 Comparative Example 3 70.5 79.8 Comparative Example 4 74.5 87.1

[0109] Table 3 Antioxidant test results of the composition for improving skin aging on the 90th day

[0110]

[0111]

[0112] It can be seen from Table 2 and Table 3 that the components of the composition for improving skin aging prepared in Examples 1 to 3 of the present invention interact with each other to enhance the scavenging of superoxide anion free radicals and hydroxyl free radicals. At the same time, the components of the composition for improving skin aging interact with each other to enhance the stability of the composition, and the anti-aging effect of the composition for improving skin aging can be well maintained.

[0113] Test Example 3: Anti-aging test of the composition for improving skin aging

[0114] 1. Test materials:

[0115] Compositions for improving skin aging were prepared by Example 2, Comparative Example 1 and Comparative Example 2.

[0116] 2. Test methods:

[0117] The detection was performed with reference to the method for determining the hydroxyproline content in the paper "Experimental Study on the Anti-Aging Effect of Chitosan on Rat Skin" published by Chen Chunye.

[0118] 2.1. Sample preparation:

[0119] 8 healthy male SD rats were selected, weighing 180-200g, and the skin of the tested parts was tested without abnormality. They were kept in separate cages and allowed to drink water freely. The backs of the SD rats were depilated and cleaned, and 4 2.0cm×2.0cm squares were drawn on the depilated parts of the SD rats and marked. The film was applied in the corresponding squares for the determination of hydroxyproline. Specifically, the composition for improving skin aging was prepared by quantitatively dropping Example 2, Comparative Example 1 and Comparative Example 2 with a dropper, and at the same time, chitosan with different molecular weights was mixed and dissolved in 1% dilute acetic acid, and the chitosan solution with a final mass fraction of 1% was added as a control group. After dropping, the chitosan solution was evenly applied once a day for 30 consecutive days. On the 31st day, the skin of the rat's back was taken, the connective tissue and fat were scraped off, the epidermis was peeled off, and the dermis was retained. Then, the dermis tissue was cut into pieces as a sample, 5 mg of dermis tissue sample was weighed and added to a 5 mL stoppered test tube, 2 mL of 6 mol / L hydrochloric acid was added, and the mixture was heated at 125°C for 2 h for hydrolysis. 1 mL of 10 mol / L sodium hydroxide was added and water was added to 5 mL to prepare a skin digestion solution for the determination of hydroxyproline content.

[0120] 2.2. Determination of Hydroxyproline Content:

[0121] 0.1 mL of skin digestion solution was taken and the content of hydroxyproline in rat skin was determined by ultraviolet spectrophotometry at 562 nm. The regression equation was calculated as follows: y = 0.000004x - 0.0032, R 2 =0.9426, there is a good linear correlation between hydroxyproline concentration and absorbance.

[0122] 3. Test results:

[0123] The test results are shown in Table 4.

[0124] Table 4 Anti-aging test results of the composition for improving skin aging

[0125] Group Quantity(n) Hydroxyproline content (ng) Control group 8 7835.574±800.325 Example 2 8 <![CDATA[20562.118±1597.342 ** ]]> Comparative Example 1 8 8152.689±1867.224 Comparative Example 2 8 <![CDATA[11564.335±1579.348 * ]]>

[0126] Note: Compared with the control group, * " indicates P < 0.05," ** ” indicates P < 0.01.

[0127] Hydroxyproline is the most important, most abundant and most stable amino acid in collagen, and its content can indirectly reflect the content of collagen fibers, thereby reflecting the degree of dermal aging. As shown in Table 4, the composition for improving skin aging provided by the present invention can significantly increase the content of hydroxyproline in rat skin tissue, thereby promoting skin collagen synthesis, enhancing skin elasticity, and having the effect of delaying skin aging.

[0128] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A composition for improving skin aging, characterized in that: Includes the following ingredients and their mass percentages: Superoxide dismutase 0.5-1.5%, carboxymethyl chitosan 1-2%, yeast extract 2-8%, emulsifier 18-25%, 1,2-pentanediol 10-15%, jojoba seed oil 10-15%, caprylic / capric triglyceride 35-45%, purified water added to 100%; The emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of (1-2):(3-6).

2. The composition for improving skin aging according to claim 1, characterized in that: The mass ratio of the superoxide dismutase to carboxymethyl chitosan is 1:(1.2-2.8).

3. The composition for improving skin aging according to claim 2, characterized in that: The mass ratio of the superoxide dismutase to carboxymethyl chitosan is 1:

2.

4. The composition for improving skin aging according to claim 2, characterized in that: The mass ratio of superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:(1.2-2.8):(2-5).

5. The composition for improving skin aging according to claim 4, characterized in that: The mass ratio of superoxide dismutase, carboxymethyl chitosan and yeast extract is 1:2:

4.

6. The composition for improving skin aging according to claim 1, characterized in that: The emulsifier is composed of sorbitan stearate and PEG-100 stearate mixed in a mass ratio of 1:

5.

7. The method for preparing the composition for improving skin aging according to any one of claims 1 to 6, characterized in that: The following steps are involved: Step S1, adding yeast extract, superoxide dismutase, carboxymethyl chitosan and 1,2-pentanediol into purified water, and stirring evenly at 350-450 rpm to obtain an aqueous phase; Step S2, mixing the emulsifier, jojoba seed oil and caprylic / capric triglyceride, and stirring at 500-600 rpm for 10-15 min to obtain an oil phase; Step S3, under stirring, adding the water phase obtained in step S1 to the oil phase obtained in step S2, and then stirring at 400-500 rpm for 20-25 min to obtain the product.

8. Use of the composition for improving skin aging according to any one of claims 1 to 6 in the preparation of anti-aging cosmetics.

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