Application of fermented mucus lactobacillus IOB802 in anti-aging

Abstract

The present invention provides an application of fermented Lactobacillus mucoides IOB802 in the direction of anti-aging, and relates to the field of microbial technology. The DPPH free radical scavenging rate of fermented Lactobacillus mucoides IOB802 is as high as 94.37-98.97%, and the hydroxyl free radical scavenging rate is as high as 97.56-99.28%, which means that it can reduce the damage of free radicals to cells and the oxidative damage caused by hydroxyl free radicals, and has in vitro antioxidant capacity. Reducing the aging manifestations of individuals is mainly reflected in delaying memory decline, weakened motor ability, decreased skin moisture content and decreased collagen content. Fermented Lactobacillus mucoides IOB802 can also enhance the antioxidant activity of blood, thereby delaying aging.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to an application of fermented mucus lactobacillus IOB802 in the direction of anti-aging. Background Art

[0002] Aging is a degeneration of the body's structure and function caused by multiple factors inside and outside the body, and the degeneration process is irreversible. Aging is mainly manifested in the degeneration of the body's biochemical indicators, stress resistance, learning and memory ability, immune function, and tissues and organs. Among them, skin aging is one of the most obvious manifestations of body aging.

[0003] Currently, there are many anti-aging methods and products on the market, but most of them have certain limitations.

[0004] Chemically synthesized anti-aging products often have the risk of side effects. Although many chemically synthesized anti-aging drugs or cosmetics can delay aging to a certain extent, they may cause adverse reactions such as skin allergies and endocrine disorders. Traditional natural anti-aging ingredients have limited effects. Some natural extracts such as vitamin C and E are widely used in anti-aging products, but their anti-aging effects are often not significant enough and their stability is poor. Existing anti-aging methods lack specificity. The aging mechanisms of different individuals are different, and most of the current anti-aging methods are broad-spectrum, making it difficult to precisely intervene in specific aging factors.

[0005] In the field of microorganisms, although people have realized that probiotics have many benefits for human health, especially lactic acid bacteria, whose effects in inhibiting harmful bacteria, regulating the intestinal environment, and enhancing immunity have been confirmed by many studies at home and abroad, there are relatively few studies on the application of lactic acid bacteria in anti-aging. The mechanism of action of some known lactic acid bacteria in anti-aging is still unclear, and its anti-aging effect needs to be further improved.

[0006] Therefore, existing anti-aging technologies and products have problems such as side effects, limited effects, and lack of specificity. There is an urgent need to develop a safe, effective, and targeted anti-aging method. The discovery of fermented Lactobacillus mucilaginosus IOB802 provides new opportunities and challenges in the anti-aging field and is expected to fill the gaps in existing anti-aging technologies. Summary of the invention

[0007] The present invention provides an application of fermented mucus lactobacillus IOB802 in the direction of anti-aging. The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum, was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120.

[0008] Preferably, the fermented Lactobacillus mucilaginosus IOB802 has at least one of the following functions:

[0009] (1) Has antioxidant capacity in vitro;

[0010] (2) Reduce the individual's signs of aging;

[0011] (3) Enhance the antioxidant activity of blood.

[0012] Preferably, the in vitro antioxidant capacity includes but is not limited to DPPH free radical scavenging capacity and hydroxyl free radical scavenging capacity; the DPPH free radical scavenging capacity is characterized by the DPPH free radical scavenging rate, and the highest DPPH free radical scavenging rate is 94.37~98.97%; the hydroxyl free radical scavenging capacity is characterized by the hydroxyl free radical scavenging rate, and the highest hydroxyl free radical scavenging rate is 97.56~99.28%.

[0013] Preferably, the aging symptoms of the individual include, but are not limited to, decreased memory, decreased motor skills, decreased skin moisture content, and decreased collagen content.

[0014] Preferably, the enhancement of blood antioxidant activity includes, but is not limited to, increasing serum total antioxidant capacity, increasing glutathione peroxidase activity and reducing malondialdehyde content.

[0015] Preferably, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare a fermented Lactobacillus mucilaginosus IOB802 bacterial preparation, a fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation and exopolysaccharide.

[0016] Preferably, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare food, health products and anti-aging medicines.

[0017] Preferably, the preparation method of the fermented Lactobacillus mucilaginosus IOB802 bacterial preparation comprises:

[0018] The fermented mucus lactobacillus IOB802 in the cryopreservation tube is inoculated into the primary culture medium at a 1% inoculation amount, and the culture is kept in a closed environment at 37°C for 22 hours to obtain a seed solution; the seed solution is inoculated into the secondary culture medium at a 1% inoculation amount, and the culture is kept in a closed environment at 37°C for 22 hours to obtain a fermented mucus lactobacillus IOB802 fermentation liquid; the fermented mucus lactobacillus IOB802 fermentation liquid is centrifuged at a speed of 5000r / min for 30 minutes at 4°C, the supernatant is discarded, the bacterial mud is pre-frozen at -80°C for 4 hours, and vacuum freeze-dried to obtain a fermented mucus lactobacillus IOB802 bacterial preparation;

[0019] Preferably, the method for preparing a postbiotic preparation of fermented Lactobacillus mucilaginosus IOB802 comprises: heat-inactivating the fermented Lactobacillus mucilaginosus IOB802 bacterial preparation at 85° C. for 15 min to obtain a postbiotic preparation of fermented Lactobacillus mucilaginosus IOB802.

[0020] Preferably, the method for preparing the extracellular polysaccharide comprises:

[0021] The fermented Lactobacillus mucilaginosus IOB802 in the cryopreservation tube was inoculated into the primary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a seed solution; the seed solution was inoculated into the secondary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a fermented Lactobacillus mucilaginosus IOB802 fermentation solution;

[0022] The fermentation liquid of Lactobacillus mucilaginosus IOB802 was heated at 100℃ for 30 min, cooled to room temperature, centrifuged at 8000 r / min for 20 min at 4℃, and the supernatant was taken; anhydrous ethanol was added to the supernatant, and the added volume was twice that of the supernatant. After standing at 4℃ overnight, centrifuged. The precipitate was redissolved in distilled water, 10% trichloroacetic acid was added, stirred at 0℃ for 4h, and the supernatant was centrifuged to adjust its pH value to 7-7.5, and 2 times the volume of anhydrous ethanol was added for alcohol precipitation. After standing at 4℃ overnight, centrifuged, the precipitate was redissolved in distilled water, and the extracellular polysaccharide was obtained after freeze-drying.

[0023] Beneficial effects of the present invention:

[0024] The present invention provides an application of fermented mucus lactobacillus IOB802 in the direction of anti-aging. On the one hand, fermented mucus lactobacillus IOB802 has in vitro antioxidant capacity, and its DPPH free radical scavenging rate is up to 94.37~98.97%, and the hydroxyl free radical scavenging rate is up to 97.56~99.28%. It means that it can reduce the damage of free radicals to cells and the oxidative damage caused by hydroxyl free radicals, so as to show antioxidant effects in vitro, maintain the normal function of cells, reduce the level of oxidative stress, reduce the damage to cell structure and function, help to delay the occurrence and development of aging, and reduce the aging characteristics of individuals, which are mainly reflected in delaying memory loss, weakened motor ability, decreased skin water content and decreased collagen content. On the other hand, fermented mucus lactobacillus IOB802 can enhance blood antioxidant activity, and there is a close connection between this characteristic and delaying aging, which is mainly manifested in improving serum total antioxidant capacity, enhancing glutathione peroxidase activity and reducing malondialdehyde content.

[0025] Glutathione peroxidase is a key enzyme in the blood antioxidant system. It plays an important role in catalyzing the reduction of hydrogen peroxide and organic hydroperoxides. The increase in its activity helps to accelerate the removal of harmful peroxides in the body and further protect cells from oxidative damage. Cells are the basic units that make up the body. The health and stability of cells is the key to delaying aging. When cells are protected from oxidative attacks, their functions can be maintained normally, thereby preventing or slowing down the aging process.

[0026] Malondialdehyde is the end product of lipid peroxidation, and its content can indirectly reflect the degree of lipid peroxidation in the body. An increase in the content of malondialdehyde indicates that after the body's cells are attacked by free radicals, lipids in structures such as cell membranes undergo peroxidation, which will destroy the integrity and normal function of the cell membrane. Fermentation of Lactobacillus mucilaginosus IOB802 reduces the content of malondialdehyde, which means that the occurrence of lipid peroxidation is reduced, the integrity of cell structures such as cell membranes is protected, the normal physiological function of cells is maintained, and ultimately helps to delay aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 To obtain the changes of platform time of mice during the training phase of water maze experiment;

[0028] Figure 2 The time the mouse stayed in the target quadrant and the number of times it crossed the platform during the test phase;

[0029] Figure 3 This is the change in the open field test data for mice;

[0030] Figure 4 is the total antioxidant capacity (T-AOC) of mouse serum;

[0031] Figure 5 is the activity of glutathione peroxidase (GSH-Px) in mouse serum;

[0032] Figure 6 is the malondialdehyde (MDA) content in mouse serum;

[0033] Figure 7 The water content and collagen content of mouse skin were measured by hydroxyproline (HYP) content;

[0034] Figure 8 Mouse skin condition.

[0035] The fermented mucus lactobacillus IOB802 used in the present invention is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120. DETAILED DESCRIPTION

[0036] The present invention provides an application of fermented mucus lactobacillus IOB802 in the direction of anti-aging. The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120.

[0037] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 has at least one of the following functions:

[0038] (1) Has antioxidant capacity in vitro;

[0039] (2) Reduce the individual's signs of aging;

[0040] (3) Enhance the antioxidant activity of blood.

[0041] In a preferred embodiment of the present invention, the in vitro antioxidant capacity includes but is not limited to DPPH free radical scavenging capacity and hydroxyl free radical scavenging capacity, the DPPH free radical scavenging capacity is characterized by the DPPH free radical scavenging rate, and the maximum DPPH free radical scavenging rate is 94.37~98.97%; the hydroxyl free radical scavenging capacity is characterized by the hydroxyl free radical scavenging rate, and the maximum hydroxyl free radical scavenging rate is 97.56~99.28%.

[0042] In a preferred embodiment of the present invention, the aging characteristics of the individual include but are not limited to decreased memory, decreased motor ability, decreased skin moisture content and decreased collagen content.

[0043] In a preferred embodiment of the present invention, the enhancement of blood antioxidant activity includes but is not limited to increasing serum total antioxidant capacity, increasing glutathione peroxidase activity and reducing malondialdehyde content.

[0044] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare a fermented Lactobacillus mucilaginosus IOB802 bacterial preparation, a fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation and exopolysaccharide.

[0045] In a preferred embodiment of the present invention, the fermented Lactobacillus mucilaginosus IOB802 can be used to prepare food, health products and anti-aging medicines.

[0046] In a preferred embodiment of the present invention, the preparation method of the fermented Lactobacillus mucilaginosus IOB802 bacterial preparation comprises:

[0047] The fermented mucus Lactobacillus IOB802 in the freezing tube was inoculated into the primary culture medium at a rate of 1%, and the culture was closed and statically cultured in a 37°C environment for 22 hours to obtain a seed solution; the seed solution was inoculated into the secondary culture medium at a rate of 1%, and the culture was closed and statically cultured in a 37°C environment for 22 hours to obtain a fermented mucus Lactobacillus IOB802 fermentation liquid; the fermented mucus Lactobacillus IOB802 fermentation liquid was centrifuged at a speed of 5000r / min for 30min at 4°C, the supernatant was discarded, the bacterial sludge was pre-frozen at -80°C for 4 hours, and then vacuum freeze-dried to obtain a fermented mucus Lactobacillus IOB802 bacterial preparation.

[0048] In the present invention, the formula of the primary culture medium and the secondary culture medium is: 20g of tryptone, 20g of glucose, 5g of sodium acetate, 2g of triammonium citrate, 1mL of Tween 80, 2g of dipotassium hydrogen phosphate, 0.1g of manganese sulfate, 0.2g of magnesium sulfate and 1000mL of distilled water are mixed, the pH is adjusted to natural, and after stirring evenly, sterilized at 121°C and 0.1MPa for 20min.

[0049] In a preferred embodiment of the present invention, the method for preparing the fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation comprises: heat-inactivating the fermented Lactobacillus mucilaginosus IOB802 bacterial preparation at 85° C. for 15 minutes to obtain the fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation.

[0050] In a preferred embodiment of the present invention, the method for preparing the extracellular polysaccharide comprises:

[0051] The fermented Lactobacillus mucilaginosus IOB802 in the cryopreservation tube was inoculated into the primary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a seed solution; the seed solution was inoculated into the secondary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a fermented Lactobacillus mucilaginosus IOB802 fermentation solution;

[0052] The fermentation broth of Lactobacillus mucilaginosus IOB802 was heated at 100°C for 30 min, cooled to room temperature, and centrifuged at 8000 r / min for 20 min at 4°C to obtain the supernatant; anhydrous ethanol was added to the supernatant in a volume twice that of the supernatant, and the mixture was allowed to stand at 4°C overnight and then centrifuged; the precipitate was redissolved in distilled water, 10% trichloroacetic acid was added, and the mixture was stirred at 0°C for 4h, and the supernatant was obtained by centrifugation, and its pH value was adjusted to 7-7.5, and 2 times the volume of anhydrous ethanol was added for re-alcohol precipitation, and the mixture was allowed to stand at 4°C overnight and then centrifuged. The precipitate was redissolved in distilled water and freeze-dried to obtain extracellular polysaccharides.

[0053] In the present invention, the formula of the primary culture medium and the secondary culture medium is: 20g of tryptone, 20g of glucose, 5g of sodium acetate, 2g of triammonium citrate, 1mL of Tween 80, 2g of dipotassium hydrogen phosphate, 0.1g of manganese sulfate, 0.2g of magnesium sulfate and 1000mL of distilled water are mixed, the pH is adjusted to natural, and after stirring evenly, sterilized at 121°C and 0.1MPa for 20min.

[0054] Example 1

[0055] Preparation method of fermented mucus lactobacillus IOB802 bacterial preparation

[0056] 1. Culture medium formula:

[0057] The primary culture medium and the secondary culture medium were modified MRS liquid culture medium, and their formula was as follows: 20 g of tryptone, 20 g of glucose, 5 g of sodium acetate, 2 g of triammonium citrate, 1 mL of Tween 80, 2 g of dipotassium hydrogen phosphate, 0.1 g of manganese sulfate, 0.2 g of magnesium sulfate and 1000 mL of distilled water were mixed, the pH was adjusted to natural, and after stirring evenly, sterilized at 121°C and 0.1 MPa for 20 min.

[0058] 2. Process

[0059] The fermented mucus Lactobacillus IOB802 in the cryopreserved tube was inoculated into the primary culture medium at a 1% inoculation amount, and was cultured in a sealed static environment at 37°C for 22 hours to obtain a seed solution. The seed solution was inoculated into the secondary culture medium at a 1% inoculation amount, and was cultured in a sealed static environment at 37°C for 22 hours to obtain a fermented mucus Lactobacillus IOB802 fermentation solution.

[0060] The fermented Lactobacillus mucilaginosus IOB802 broth was centrifuged at 5000 rpm for 30 min at 4° C., the supernatant was discarded, the bacterial sludge was pre-frozen at -80° C. for 4 h, and then vacuum freeze-dried to obtain the fermented Lactobacillus mucilaginosus IOB802 bacterial preparation.

[0061] 3. Activity detection

[0062] According to the method of GB4789.352024 National Food Safety Standard Food Microbiology Test Lactic Acid Bacteria Test, the final test result was 3.60×10 10 CFU / g.

[0063] Example 2

[0064] Preparation of postbiotic preparations from fermented Lactobacillus mucilaginosus IOB802

[0065] The fermented Lactobacillus mucilaginosus IOB802 bacterial preparation in Example 1 was heat-inactivated at 85° C. for 15 min to obtain a fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation.

[0066] Example 3

[0067] Extraction of exopolysaccharide from fermented Lactobacillus mucilaginosus IOB802

[0068] 1. Culture medium formula:

[0069] The primary culture medium and the secondary culture medium were modified MRS liquid culture medium, and their formula was as follows: 20 g of tryptone, 20 g of glucose, 5 g of sodium acetate, 2 g of triammonium citrate, 1 mL of Tween 80, 2 g of dipotassium hydrogen phosphate, 0.1 g of manganese sulfate, 0.2 g of magnesium sulfate and 1000 mL of distilled water were mixed, the pH was adjusted to natural, and after stirring evenly, sterilized at 121°C and 0.1 MPa for 20 min.

[0070] 2. Process

[0071] The fermented Lactobacillus mucilaginosus IOB802 in the cryopreservation tube was inoculated into the primary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a seed solution; the seed solution was inoculated into the secondary culture medium at a 1% inoculation amount, and was cultured in a sealed environment at 37°C for 22 hours to obtain a fermented Lactobacillus mucilaginosus IOB802 fermentation solution;

[0072] The fermentation broth of Lactobacillus mucilaginosus IOB802 was heated at 100℃ for 30 min, cooled and centrifuged (4℃, 8000 r / min, 20 min) to obtain the supernatant. Anhydrous ethanol was added to the supernatant in a volume twice that of the supernatant, and the mixture was allowed to stand at 4℃ overnight and then centrifuged; the precipitate was redissolved in distilled water, 10% trichloroacetic acid was added, and the mixture was stirred at 0℃ for 4 h, and the supernatant was centrifuged to obtain the supernatant, and the pH value was adjusted to 7-7.5, and 2 times the volume of anhydrous ethanol was added for alcohol precipitation, and the mixture was allowed to stand at 4℃ overnight and then centrifuged. The precipitate was redissolved in distilled water and freeze-dried to obtain the extracellular polysaccharide.

[0073] Example 4

[0074] In vitro study on the antioxidant activity of fermented Lactobacillus mucilaginosus IOB802

[0075] 1. DPPH free radical scavenging rate determination

[0076] Preparation of test samples: Fermented Lactobacillus mucilaginosus IOB802 bacterial preparation was diluted with normal saline to a high dose of 10 9 CFU / mL, medium dose 10 8 CFU / mL and low dose 10 7 CFU / mL, fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation was diluted in the same proportion, i.e., diluted with normal saline to a high dose of 10 9 CFU / mL, medium dose 10 8 CFU / mL, low dose 10 7 CFU / mL, fermented Lactobacillus mucilaginosus IOB802 exopolysaccharide was diluted with normal saline to a high dose of 5 mg / mL, a medium dose of 0.5 mg / mL, and a low dose of 0.05 mg / mL.

[0077] Add 0.1 mL of the sample to be tested and 0.1 mL of 0.2 mmol / L DPPH-ethanol solution to the test tubes, mix thoroughly, and react at room temperature in the dark for 30 min. Take distilled water as the blank group, measure the absorbance at 517 nm, and repeat three times for each group. Take 0.1 mL of saline and 0.1 mL of anhydrous ethanol as the control group, and calculate the DPPH free radical scavenging rate according to formula ①.

[0078] DPPH free radical scavenging rate (%) = [A0–(A1–A2)] / A0×100%①

[0079] Where: A0 is the absorbance of the blank group, A1 is the absorbance of the sample group, and A2 is the absorbance of the control group (absolute ethanol is used instead of DPPH-ethanol solution).

[0080] Table 1 DPPH free radical scavenging rate

[0081]

[0082] 2. Hydroxyl free radical scavenging rate determination

[0083] Preparation of test samples: Fermented Lactobacillus mucilaginosus IOB802 bacterial preparation was diluted with normal saline to a high dose of 10 9 CFU / mL, medium dose 10 8 CFU / mL, low dose 10 7 CFU / mL, fermented Lactobacillus mucilaginosus IOB802 postbiotic preparation was diluted in the same proportion, i.e., diluted with normal saline to a high dose of 10 9 CFU / mL, medium dose 10 8 CFU / mL, low dose 10 7 CFU / mL, fermented Lactobacillus mucilaginosus IOB802 exopolysaccharide was diluted with normal saline to a high dose of 5 mg / mL, a medium dose of 0.5 mg / mL, and a low dose of 0.05 mg / mL.

[0084] Take 0.1 mL of the sample to be tested, add 0.1 mL each of 6 mmol / L FeSO4 solution and 6 mmol / L H2O2 solution, mix thoroughly, let stand at room temperature for 10 min, then add 0.1 mL of 6 mmol / L salicylic acid solution and mix thoroughly; let stand at room temperature for 30 min, then measure the absorbance at 510 nm, and repeat three times for each group.

[0085] The control group was as follows: 0.1 mL of normal saline was taken, 0.1 mL of 6 mmol / L FeSO4 solution and 0.1 mL of 6 mmol / L H2O2 solution were added, the mixture was mixed thoroughly, and after standing at room temperature for 10 min, 0.1 mL of 6 mmol / L distilled water was added and the mixture was mixed thoroughly; after standing at room temperature for 30 min, the absorbance was measured at 510 nm.

[0086] The hydroxyl radical scavenging rate was calculated according to formula ②.

[0087] Hydroxyl free radical scavenging rate (%) = 1-[(A1-A2) / A0]×100%②

[0088] Where: A0 is the absorbance of the blank group, A1 is the absorbance of the sample, and A2 is the absorbance of the control group (distilled water is used instead of salicylic acid).

[0089] Table 2 Hydroxyl radical scavenging rate

[0090]

[0091] The high-dose fermented Lactobacillus muciniphila IOB802 bacterial preparation, postbiotic preparation and exopolysaccharide had high scavenging rates for DPPH free radicals and hydroxyl free radicals. It can be seen that the fermented Lactobacillus muciniphila IOB802 bacterial preparation, postbiotic preparation and exopolysaccharide all had in vitro antioxidant activity.

[0092] Example 5

[0093] Effects of fermented Lactobacillus mucilaginosus IOB802 on memory in aging mice

[0094] Four-week-old SPF male Kunming mice weighing 18±2 g were selected. After 7 days of adaptive feeding, they were randomly divided into 7 groups, with 6 mice in each group. Except for the normal group, mice in the other groups were subcutaneously injected with 200 mg / (kg·d) D-galactose in the back of the neck every day to establish an aging model. The mice in the normal group were injected with an equal volume of saline every day. The mice in the high-dose bacterial preparation group were gavaged with 0.2 mL10 9 CFU / mL of fermented mucus Lactobacillus IOB802 bacterial preparation, the mice in the low-dose group were gavaged with 0.2 mL10 8 CFU / mL fermented mucus Lactobacillus IOB802 bacterial preparation; mice in the high-dose group of postbiotic preparation were gavaged with 0.2 mL10 9 CFU / mL of fermented mucus Lactobacillus IOB802 postbiotic preparation, the mice in the low-dose group of postbiotic preparation were gavaged with 0.2 mL10 8 CFU / mL fermented Lactobacillus mucosa IOB802 postbiotic preparation; the exopolysaccharide group was gavaged with 0.2 mL 5 mg / mL exopolysaccharide; the normal group and model group mice were gavaged with an equal volume of normal saline daily for 5 consecutive weeks. During the experiment, the mice were free to eat and drink.

[0095] The water maze experiment lasted for 6 days. The first 5 days were trained at a fixed time. The mice were randomly placed in the water with their heads facing the pool wall at one of the four starting positions: east, west, south, and north. The time (s) for the mice to find the underwater platform was recorded. In the first 2 days of training, if this time exceeded 60s, the animals were guided to the platform and allowed to stay on the platform for 10s. After the training was completed, the mice were removed, wiped clean, dried under air conditioning, and returned to the cage. Each animal was trained 4 times a day, with an interval of 15-20min between two training sessions.

[0096] The changes in the time it takes for mice to obtain the platform during the first 5 days of training are as follows: Figure 1 As shown, the time taken by the model group mice to obtain the platform was significantly longer than that of the normal group, which proved that the learning and memory ability of the model group mice declined and the aging model was successfully established. The low-dose group of fermented mucus Lactobacillus IOB802 bacterial preparation and the low-dose group of postbiotic preparation significantly shortened the time taken by mice to obtain the platform, and on the 5th day, it was close to the time taken by the normal group mice to obtain the platform.

[0097] On the 6th day, the platform was removed and the mouse was placed in the water from the opposite side of the original platform quadrant. The time (s) the mouse spent in the target quadrant (i.e., the quadrant where the platform was originally placed) and the number of times it entered the quadrant were recorded.

[0098] On the 6th day of the test phase, the time mice stayed in the target quadrant and the number of times they crossed the platform were as follows: Figure 2 Similar to the exopolysaccharide group, after treatment with fermented Lactobacillus mucilaginosus IOB802 bacterial preparation and postbiotic preparation, the target quadrant residence time and platform crossing times of mice were significantly higher than those of the model group, and were close to those of the normal group, indicating that the learning and memory abilities of mice were improved to varying degrees.

[0099] Example 6

[0100] Effects of fermented Lactobacillus mucilaginosus IOB802 on the locomotor activity of aging mice

[0101] The animal experiment design and gavage groups involved in this embodiment are the same as those in Example 5, and the open field experiment was carried out starting from the 5th week. Place the box in a quiet environment, and start recording and timing when the mouse is placed in the center of the box. After 5 minutes, take out the mouse, stop recording, and clean the box to ensure that there is no residual information such as odor in the box. Replace the mouse and repeat the recording and timing steps. According to the video, count the movement time (s), residence time (s), and total movement distance (m) of each mouse in the center area.

[0102] like Figure 3 As shown, compared with the normal group, the total movement distance, average moving speed and central area movement time of the aged mice in the model group decreased, while the residence time increased, indicating that the autonomous movement and exploratory behavior of the aged mice to the new environment decreased; compared with the model group, after treatment with the fermented Lactobacillus mucus IOB802 bacterial preparation and the postbiotic preparation, the open field test indicators of the mice were better than those of the model group.

[0103] Example 7

[0104] Effects of fermented Lactobacillus mucilaginosus IOB802 on serum antioxidant capacity of aging mice

[0105] The gavage time involved in this example was extended to 6 weeks, and the rest of the animal experimental design and gavage groups were the same as in Example 5. The total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content of mouse serum were determined according to the kit experimental method.

[0106] like Figure 4 , Figure 5 and Figure 6As shown, compared with the model group, the activities of T-AOC and GSH-Px in the serum of mice treated with fermented Lactobacillus mucinous IOB802 bacterial preparations and postbiotic preparations by gavage were significantly increased, and the content of MDA, a product of lipid peroxidation, was significantly decreased, indicating that fermented Lactobacillus mucinous IOB802 bacterial preparations and postbiotic preparations can exert strong antioxidant capacity in mice and delay the aging of mice.

[0107] Example 8

[0108] Effects of fermented Lactobacillus mucilaginosus IOB802 on skin moisture and collagen content in aging mice

[0109] The animal experiment design and gavage groups involved in this example are the same as those in Example 5. Skin water content and collagen content are the main reference indicators for judging the degree of skin aging. The skin water content of mice is detected using a skin moisture detector, and the collagen content is indirectly estimated by the hydroxyproline (HYP) content. The hydroxyproline content detection is performed according to the kit method.

[0110] The water content and collagen content of mouse skin were measured by hydroxyproline (HYP) content. Figure 7 As shown, the skin water content and collagen content of the fermented Lactobacillus mucus IOB802 bacterial preparation group and the fermented Lactobacillus mucus IOB802 postbiotic preparation group were higher than those of the model group, proving that the fermented Lactobacillus mucus IOB802 bacterial preparation and postbiotic preparation can slow down the loss of water and collagen in the mouse skin.

[0111] Example 9

[0112] Effects of fermented Lactobacillus mucilaginosus IOB802 on skin tissue of aging mice

[0113] The animal experiment design and gavage groups involved in this example are the same as those in Example 5. The skin tissues of the backs of the mice were taken for HE staining and Masson staining to directly observe the skin condition. The skin tissues of the mice in each group were fixed and stained and observed. The results are as follows: Figure 8 As shown. The boundary between the epidermis and dermis of the model group mice was unclear, the dermal fiber tissue was loosely arranged and disordered, and the collagen fiber content was significantly reduced. After intervention with fermented mucus lactobacillus IOB802 bacterial preparations and postbiotic preparations, the skin tissue of the mice improved, the dermal fiber tissue structure was restored, and the collagen fiber content increased. This proves that fermented mucus lactobacillus IOB802 bacterial preparations and postbiotic preparations have delayed skin aging to a certain extent.

Claims

1. An application of fermented Lactobacillus mucilaginosus IOB802 in the preparation of health foods and anti-aging drugs that are helpful for anti-oxidation, characterized in that: The fermented mucus lactobacillus IOB802 is classified and named as fermented mucus lactobacillus Limosilactobacillus fermentum , was deposited on August 5, 2021 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC, with the address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23120.

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