Composition containing samaras oil for assisting in lowering blood lipids and preparation method thereof

Abstract

The present invention relates to the technical field of reducing blood lipids, and in particular to a composition containing samara oil for assisting in reducing blood lipids and a preparation method thereof. The composition prepared by the present invention using samara oil, red yeast rice, taurine, bamboo leaf flavonoids, thread-leaf broom extract, Lactobacillus reuteri GMNL-263, erythritol, resistant dextrin, phospholipids and oligofructose as raw materials has a good effect, uses edible substances as raw materials, can be used daily, has low impact on human body functions, and also has a high efficiency in assisting in reducing blood lipids.

Description

Technical Field

[0001] The invention relates to the technical field of lowering blood lipids, and in particular to a composition containing samaras oil for assisting in lowering blood lipids and a preparation method thereof. Background Art

[0002] Cardiovascular and cerebrovascular diseases are manifestations of systemic vascular diseases or systemic vascular diseases in the heart and brain. There are four main causes: vascular factors such as atherosclerosis, hypertensive arteriosclerosis, and arteritis; hemodynamic factors such as hypertension; blood rheology abnormalities such as hyperlipidemia and diabetes; and blood component factors such as leukemia, anemia, and thrombocytosis.

[0003] Hyperlipidemia is the culprit for repeated increases in blood pressure and an important inducing factor for cardiovascular and cerebrovascular diseases. Hyperlipidemia can cause lipids to deposit on the arterial wall, forming plaques, which in turn causes the arterial wall to become hard and deformed. Hyperlipidemia is an important risk factor for coronary heart disease, which can cause coronary artery sclerosis, affect myocardial blood supply, lead to myocardial ischemia, and thus cause diseases such as angina pectoris and myocardial infarction. Hyperlipidemia increases the risk of thrombosis, leading to stroke. Hyperlipidemia can lead to aggravated inflammatory reactions in blood vessels and affect the patency of blood vessels. Arteriosclerosis and lipid deposition caused by hyperlipidemia increase the pressure exerted by blood on the blood vessel wall, leading to high blood pressure. Long-term hyperlipidemia may cause damage to pancreatic islet cells and abnormal insulin secretion, thus causing insulin resistance and diabetes. Hyperlipidemia is one of the important factors leading to obesity. Hyperlipidemia can lead to liver diseases such as fatty liver, liver fibrosis, and cirrhosis. Long-term hyperlipidemia can impair immune function and increase the risk of diseases such as cancer. Hyperlipidemia can lead to neurological diseases such as Parkinson's disease and Alzheimer's disease.

[0004] There are many drugs for treating hyperlipidemia, but long-term use will bring more side effects and affect the normal function of the human body. Therefore, it is a technical problem that needs to be solved to provide a composition that has good auxiliary effect in lowering blood lipids and can be used daily and has little effect on the body. Summary of the invention

[0005] The present invention is a solution to the above technical problems. The present invention proposes a composition containing samaras oil for assisting in reducing blood lipids and a preparation method thereof. The present invention uses edible raw materials to prepare the composition, which can be used daily; the raw materials are derived from food and have little effect on human body functions; and it also has a highly effective effect of assisting in reducing blood lipids.

[0006] On the one hand, the present invention provides a composition containing samaras oil for assisting in lowering blood lipids. The raw materials for preparing the composition include samaras oil, red yeast rice, taurine, bamboo leaf flavonoids, broom extract, Lactobacillus reuteri GMNL-263, erythritol, resistant dextrin, phospholipids and oligofructose.

[0007] Furthermore, the samara oil used in the present invention can be in the form of powder, liquid or other forms, or in the form of samara oil microcapsule powder.

[0008] Furthermore, water can be used as a raw material for preparing the composition.

[0009] Furthermore, the composition containing samara oil for assisting in lowering blood lipids includes the following preparation raw materials: 3-15g samara oil, 0.1-0.5g red yeast rice, 0.01-0.05g taurine, 0.5-2.5g bamboo leaf flavonoids, 2-4g broom extract, Lactobacillus reuteri GMNL-263, 0.1-0.4g erythritol, 8-50g resistant dextrin, 3-6g phospholipids, and 0.2-0.8g oligofructose.

[0010] Furthermore, the mass ratio of the samarassa oil to the broom extract is 1.5-2.5:1.

[0011] Furthermore, the composition includes the following raw materials, in parts by mass: 3.5-13.5g of samarass oil, 0.15-0.3g of red yeast rice, 0.01-0.03g of taurine, 0.8-2.3g of bamboo leaf flavonoids, 2.5-3.7g of broom extract, Lactobacillus reuteri GMNL-263, 0.1-0.3g of erythritol, 10-48g of resistant dextrin, 3.2-5.5g of phospholipids, and 0.25-0.6g of oligofructose.

[0012] Furthermore, the composition is in the form of powder, granules or tablets.

[0013] Further, other plant extract oil raw materials can be used according to functional requirements, such as 0.1-6g of perilla seed oil, etc. The perilla seed oil used can be added in powder, liquid or other forms in combination with dosage form requirements.

[0014] Furthermore, the preparation method of the Broom Flower Extract comprises the following steps:

[0015] Step 1: crush the dried broom to obtain powder, add water, stir at 50-70° C. for 6-15 hours, filter to obtain a filtrate and a filter residue, and freeze-dry the filtrate to obtain an intermediate material;

[0016] Step 2: Mix the filter residue with an ethanol aqueous solution having a concentration of 65-78 wt%, stir at 40-55° C. for 5-12 hours, filter to obtain a filter solution, and then concentrate under reduced pressure to obtain a concentrate;

[0017] Step 3: Mix the concentrate with an extractant composed of ethyl acetate and water in a mass ratio of 4-6:1, shake well, and extract at a temperature of 35-45° C. for 4-10 hours, stand to separate the layers, then pour out the water layer, and concentrate the remaining material under reduced pressure until it does not contain ethyl acetate to obtain a material;

[0018] Step 4: Mix the intermediate material and the material to obtain the broom extract.

[0019] Furthermore, in step 2, the solution is concentrated under reduced pressure to 8-15% of the mass of the filtered solution.

[0020] Furthermore, in step 1, the mass ratio of powder to water is 1:20-50.

[0021] Furthermore, in step 2, the mass ratio of the filter residue to the ethanol aqueous solution is 1:15-40.

[0022] Furthermore, in step 3, the mass ratio of the concentrate to the extractant is 1:10-30.

[0023] Furthermore, in step 3, the temperature of the reduced pressure concentration is 50-55°C and the pressure is 4.5×10 3 -7.2×10 3 Pa.

[0024] On the other hand, the present invention also provides a method for preparing the composition, comprising the following steps:

[0025] S1: mixing lecithin, samaras oil, partially resistant dextrin, and broom extract with hot water, and spray drying to obtain a first mixture;

[0026] S2: mixing oligofructose, erythritol, remaining resistant dextrin, Lactobacillus reuteri and water, and freeze-drying to obtain a second mixture;

[0027] S3: Red yeast rice, taurine, brassica juncea, the first mixture and the second mixture are combined, and then crushed and sterilized to obtain the composition.

[0028] Furthermore, in S1, the portion of resistant dextrin accounts for 30%-70% of the total mass of resistant dextrin.

[0029] Furthermore, in S1, the temperature of the hot water is 50-65°C.

[0030] Furthermore, in S2, Lactobacillus reuteri can be added in the form of live bacteria or non-live bacteria.

[0031] Furthermore, the present invention uses Lactobacillus reuteri GMNL-263 as a functional ingredient, and its usage amount can be conventionally selected according to the functional strength requirements.

[0032] Furthermore, in S2, the amount of Lactobacillus reuteri added can be 8×10 3 -6.5×10 9 CFU / 10g total amount of non-aqueous raw materials. Non-aqueous raw materials refer to all raw materials other than water in the preparation of raw materials.

[0033] Beneficial effects:

[0034] The composition prepared by the present invention, which includes wing nut oil, red yeast rice, taurine, bamboo leaf flavonoids, broom extract, Lactobacillus reuteri GMNL-263, erythritol, resistant dextrin, phospholipids and oligofructose, has good effect, uses edible substances as raw materials, can be used daily, has low impact on human body functions, and has a high efficiency in assisting the effect of lowering blood lipids.

[0035] The composition prepared by the present invention has a good auxiliary lipid-lowering effect. In the hyperlipidemia model test, the composition of the present invention was used for 25 days at a dosage of 1.2g / kg mice, which can reduce the TG, TC, and LDL-C content in the mouse serum to 2.18±0.17mmol / L, 5.15±0.45mmol / L, and 0.72±0.07mmol / L, and increase the HDL-C content in the mouse serum to 2.21±0.27mmol / L. And compared with the mouse serum in the model group, the reduction rates of TG, TC, and LDL-C were 36.26%, 29.55%, and 28.71%, and the increase rate of HDL-C content was 33.13%.

[0036] The process for extracting the broom flower extract of the present invention has a certain influence on the auxiliary blood lipid lowering effect of the product; and in the process of using ethanol aqueous solution for extraction, when the mass concentration of the ethanol aqueous solution is 65%-78%, the auxiliary blood lipid lowering effect is better; and the effect of the extractant composed of ethyl acetate used in the present invention is better.

[0037] The present invention adopts a specific process to obtain the genus smilax extract and the wing fruit oil to form a cooperative relationship, which can synergistically promote the auxiliary blood lipid lowering effect of the composition to a certain extent. When the ratio of the two is in the range of 1.5-2.5:1, the product shows better reduction of TG, TC, LDL-C content in serum and better increase of HDL-C content.

[0038] The present invention adopts Lactobacillus reuteri GMNL-263, which can make the product have better effects of reducing TG, TC, and LDL-C contents in serum and better increasing HDL-C content. DETAILED DESCRIPTION

[0039] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0040] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0041] 1. Preparation of Broom Extract

[0042] Broom extract 1: The preparation steps are as follows:

[0043] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 30 times the mass of water, stir at 65°C and 100 rpm for 10 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -30°C to a water content of 3.47% to obtain an intermediate material 1;

[0044] Step 2: the filter residue was mixed with a 75wt% ethanol aqueous solution in a mass ratio of 1:28, stirred at 50°C for 10 hours, filtered through a 0.22μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 52°C to 12.5% ​​of the mass of the filtered solution to obtain a concentrate;

[0045] Step 3: The concentrate was mixed with an extractant consisting of ethyl acetate and water in a mass ratio of 5:1 in a mass ratio of 1:20, shaken and extracted at a temperature of 40°C for 7.2 hours, allowed to stand and stratify, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions, concentrated under reduced pressure until no ethyl acetate was present, to obtain material 2;

[0046] Step 4: Mix the intermediate material 1 and the material 2 to obtain the broom extract 1.

[0047] Broom extract 2: Preparation steps are as follows:

[0048] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 25 times the mass of water, stir at 58°C and 100 rpm for 14 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -28°C to a water content of 3.38% to obtain an intermediate material 1;

[0049] Step 2: the filter residue was mixed with a 65wt% ethanol aqueous solution at a mass ratio of 1:30, stirred at 52°C for 9 hours, filtered through a 0.22μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 53°C to 12% of the mass of the filtered solution to obtain a concentrate;

[0050] Step 3: The concentrate was mixed with an extractant consisting of ethyl acetate and water in a mass ratio of 4.5:1 in a mass ratio of 1:25, shaken and extracted at a temperature of 42°C for 7 hours, allowed to stand and stratified, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions, concentrated under reduced pressure until no ethyl acetate was present, to obtain material 2;

[0051] Step 4: Mix the intermediate material 1 and the material 2 to obtain the broom extract 2.

[0052] Broom extract 3: Preparation steps are as follows:

[0053] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 30 times the mass of water, stir at 65°C and 100 rpm for 10 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -30°C to a water content of 3.47% to obtain an intermediate material 1;

[0054] Step 2: The filter residue was mixed with a 55wt% ethanol aqueous solution in a mass ratio of 1:28, stirred at 50°C for 10 hours, filtered through a 0.22μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 52°C to 12.5% ​​of the mass of the filtered solution to obtain a concentrate;

[0055] Step 3: The concentrate was mixed with an extractant consisting of ethyl acetate and water in a mass ratio of 5:1 in a mass ratio of 1:20, shaken and extracted at a temperature of 40°C for 7.2 hours, allowed to stand and stratify, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions, concentrated under reduced pressure until no ethyl acetate was present, to obtain material 2;

[0056] Step 4: Mix the intermediate material 1 and the material 2 to obtain the broom extract 3.

[0057] Broom extract 4: The preparation steps are as follows:

[0058] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 30 times the mass of water, stir at 65°C and 100 rpm for 10 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -30°C to a water content of 3.47% to obtain an intermediate material 1;

[0059] Step 2: the filter residue was mixed with an ethanol aqueous solution having a concentration of 87 wt % in a mass ratio of 1:28, stirred at 50° C. for 10 hours, filtered through a 0.22 μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 52° C. to 12.5% ​​of the mass of the filtered solution to obtain a concentrate;

[0060] Step 3: The concentrate was mixed with an extractant consisting of ethyl acetate and water in a mass ratio of 5:1 in a mass ratio of 1:20, shaken and extracted at a temperature of 40°C for 7.2 hours, allowed to stand and stratify, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions, concentrated under reduced pressure until no ethyl acetate was present, to obtain material 2;

[0061] Step 4: Mix the intermediate material 1 and the material 2 to obtain the broom extract 4.

[0062] Broom extract 5: Preparation steps are as follows:

[0063] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 30 times the mass of water, stir at 65°C and 100 rpm for 10 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -30°C to a water content of 3.47% to obtain an intermediate material 1;

[0064] Step 2: the filter residue was mixed with a 75wt% ethanol aqueous solution in a mass ratio of 1:28, stirred at 50°C for 10 hours, filtered through a 0.22μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 52°C to 12.5% ​​of the mass of the filtered solution to obtain a concentrate;

[0065] Step 3: The concentrate was mixed with an extractant composed of cyclohexane and water in a mass ratio of 5:1 in a mass ratio of 1:20, shaken and extracted at a temperature of 40°C for 7.2 hours, allowed to stand and stratify, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions and concentrated under reduced pressure until cyclohexane-free to obtain material 2;

[0066] Step 4: Mix the intermediate material 1 and the material 2 to obtain the Broom extract 5.

[0067] Broom extract 6: Preparation steps are as follows:

[0068] Step 1: Crush the dried broom to obtain powder (particle size is 0.05-1 mm), add 30 times the mass of water, stir at 65°C and 100 rpm for 10 hours, filter with a 0.22 μm pore size filter membrane to obtain a filtrate and a filter residue, and freeze-dry the filtrate at -30°C to a water content of 3.47% to obtain an intermediate material 1;

[0069] Step 2: the filter residue was mixed with a 75wt% ethanol aqueous solution in a mass ratio of 1:28, stirred at 50°C for 10 hours, filtered through a 0.22μm pore size filter membrane to obtain a filtered solution, and then concentrated under reduced pressure at 52°C to 12.5% ​​of the mass of the filtered solution to obtain a concentrate;

[0070] Step 3: The concentrate was mixed with an extractant composed of chloroform and water in a mass ratio of 5:1 in a mass ratio of 1:20, shaken and extracted at a temperature of 40°C for 7.2 hours, allowed to stand and stratify, and then the water layer was poured out, and the remaining material was 52°C, 5.3×10 3 Pa conditions, concentrated under reduced pressure until chloroform was not contained, to obtain material 2;

[0071] Step 4: Mix the intermediate material 1 and the material 2 to obtain the broom extract 6.

[0072] 2. Preparation of the composition

[0073] Source of raw materials: wing fruit oil: Shanxi Qierkang Wing Fruit Biological Products Co., Ltd., each 100g contains oleic acid 30.0g, linoleic acid 45.0g, linolenic acid 6.0g, vitamin E 987mg; red yeast rice: CAS NO: 97404-52-9, food grade, Henan Kangzhiwang Biotechnology Co., Ltd.; taurine: CAS NO: 107-35-7, food grade, Xi'an Tianfeng Biotechnology Co., Ltd.; bamboo leaf flavonoids: CAS NO: 91771-33-4, food grade, Nanjing Puyi Biotechnology Co., Ltd.; erythritol CAS NO: 149-32-6, food grade, Taizhou Jufengyuan Biotechnology Co., Ltd.; resistant dextrin: CAS NO: 9004-53-9, food grade, Zhengzhou Yuhe Food Additive Co., Ltd.; phospholipids: soybean lecithin, CAS NO: 8002-43-5, food grade, Henan Zhuogu Biotechnology Co., Ltd.; oligofructose: CAS NO: 308066-66-2, food grade, Nantong Tianxiang Bioengineering Co., Ltd.; Lactobacillus reuteri GMNL-263: Jingyue Biotechnology Co., Ltd.

[0074] 1. Composition 1: The preparation steps are as follows:

[0075] S1: weigh the raw materials: 5g samara oil, 0.18g red yeast rice, 0.02g taurine, 1.5g bamboo leaf flavonoids, 2.5g genus broom extract 1, 0.1g erythritol, 45g resistant dextrin, 3.9g phospholipids and 0.3g oligofructose; wherein the mass ratio of samara oil to the genus broom extract 1 is 2:1; Lactobacillus reuteri GMNL-263 is fed in the form of an aqueous solution, and the concentration of the bacteria in the aqueous solution is 1.12×10 9 CFU / g, the amount of feed solution was 10 g;

[0076] The lecithin, samaras oil, part of the resistant dextrin (50% of the total mass of the resistant dextrin), and the extract of the genus Glechoma longituba were mixed with hot water at 58° C., stirred at 200 rpm for 1 hour, and spray-dried to a water content of 3.27% to obtain a first mixture 1;

[0077] S2: mixing oligofructose, erythritol, remaining resistant dextrin, Lactobacillus reuteri aqueous solution and water at 70 rpm for 0.5 hour, and freeze-drying to a water content of 3.59% to obtain a second mixture 2;

[0078] S3: Red yeast rice, taurine, bamboo leaf brass, the first mixture 1 and the second mixture 2 are combined, then crushed, particles with a particle size between 20 mesh and 100 mesh are selected, and sterilized to obtain the composition.

[0079] 2. Composition 2: The preparation steps are as follows:

[0080] S1: Weigh the raw materials: 5.4g samara oil, 0.15g red yeast rice, 0.01g taurine, 1.5g bamboo leaf flavonoids, 2.3g line leaf broom extract 2, 0.11g erythritol, 48g resistant dextrin, 3.2g phospholipids and 0.25g oligofructose; wherein the mass ratio of samara oil to the line leaf broom extract 2 is 2.35:1; Lactobacillus reuteri GMNL-263 is fed in the form of an aqueous solution, and the concentration of the bacteria in the aqueous solution is 1.2×10 9 CFU / g, the amount of feed solution was 10 g;

[0081] The lecithin, samaras oil, part of the resistant dextrin (60% of the total mass of the resistant dextrin), and the extract of Glechoma longituba were mixed with hot water at 60° C., stirred at 180 rpm for 0.8 hours, and spray-dried to a water content of 3.35% to obtain a first mixture 1;

[0082] S2: mixing oligofructose, erythritol, remaining resistant dextrin, Lactobacillus reuteri aqueous solution and water at 80 rpm for 0.5 hour, and freeze-drying to a water content of 3.78% to obtain a second mixture 2;

[0083] S3: Red yeast rice, taurine, bamboo leaf brass, the first mixture 1 and the second mixture 2 are combined, then crushed, particles with a particle size between 20 mesh and 100 mesh are selected, and sterilized to obtain the composition.

[0084] 3. Composition 3: The only difference from composition 1 is that the broom extract 3 is used to replace the broom extract 1, and the rest are the same.

[0085] 4. Composition 4: The only difference from composition 1 is that the broom extract 4 is used to replace the broom extract 1, and the rest are the same.

[0086] 5. Composition 5: The only difference from composition 1 is that the broom extract 5 is used to replace the broom extract 1, and the rest are the same.

[0087] 6. Composition 6: The only difference from composition 1 is that the broom extract 6 is used to replace the broom extract 1, and the rest are the same.

[0088] 7. Composition 7: The only difference from composition 1 is that samaras oil is not used, the amount of the broom extract 1 is 7.5 g, and the rest are the same.

[0089] 8. Composition 8: The only difference from composition 1 is that the broom extract 1 is not used, the samaras oil is 7.5 g, and the rest are the same.

[0090] 9. Composition 9: The only difference from Composition 1 is that Composition 9 uses 2.5 g of wingnut oil and 5 g of the broom extract 1, and the ratio of the two is 0.5:1. Other components are the same.

[0091] 10. Composition 10: The only difference from composition 1 is that composition 10 uses 6 g of wingnut oil and 1.5 g of the broom extract 1, and the ratio of the two is 4:1. Other components are the same.

[0092] 11. Composition 11: The only difference between composition 1 and composition 1 is that composition 11 does not use Lactobacillus reuteri GMNL-263, and all other aspects are the same.

[0093] 3. Effect Test

[0094] Experimental subjects: 18±2g male Kunming mice.

[0095] High-fat feed: 1.2wt% cholesterol, 9.5wt% egg yolk powder, 10wt% lard, 0.25wt% bile salt and the remainder basic feed.

[0096] Experimental process:

[0097] Adaptive feeding: mice were fed with basic feed in an environment of 25±2℃ and relative humidity of 50±5% for one week;

[0098] Grouping: The adaptive pattern mice were randomly grouped according to body weight into a blank group, a model control group, a positive control group, and combination groups 1-11, a total of 14 groups, with 10 mice in each group.

[0099] Hyperlipidemia model establishment: Except for the blank group which was fed with basic feed, the mice in other groups were fed with high-fat feed for about 30 days in an environment of 25±2℃ and relative humidity of 50±5%. The serum TC and TG levels were measured to determine the hour when hyperlipidemia was formed in this group.

[0100] Drug administration: After the modeling was completed, each group of mice was given drug treatment; the drugs in the positive control group and the combination group were diluted with normal saline to form a suspension and then gavaged. The dosage of crude drug in the positive control group was 0.063 g / kg mouse (the drug was diluted with normal saline, and the gavage volume was 0.1 mL / 10 g mouse. The drug was Jiangzhiling Tablets (Tai Chi), product number 14002358005), and the dosage of crude drug in the combination group was 1.2 g / kg mouse (the above-mentioned composition 1-11 was diluted with normal saline, and the gavage volume was 0.1 mL / 10 g mouse); the model group and the blank group were gavaged with normal saline equal to the suspension; gavage was continued for 25 days, and the environment was 25±2°C and the relative humidity was 50±5%.

[0101] Detection: After 25 days of administration, the mice were fasted for 16 hours and blood was collected to detect the TG, TC, HDL-C, and LDL-C parameter values ​​in the serum. The results are shown in Table 1.

[0102] Table 1 Parameter values ​​of TG, TC, LDL-C and HDL-C in mouse serum

[0103] test TG / mmol / L TC / mmol / L LDL-C / mmol / L HDL-C / mmol / L Blank group 1.23±0.24 4.69±0.62 0.53±0.06 2.35±0.21 Model Group 3.42±0.39 7.31±0.74 1.01±0.12 1.66±0.19 Positive control group 2.43±0.27 5.26±0.57 0.83±0.04 2.14±0.24 Composition 1 set 2.18±0.17 5.15±0.45 0.72±0.07 2.21±0.27 Composition 2 sets 2.20±0.28 5.19±0.78 0.73±0.08 2.20±0.18 Composition 3 groups 2.34±0.33 5.44±0.63 0.78±0.09 2.13±0.23 Composition 4 groups 2.25±0.21 5.27±0.55 0.74±0.10 2.17±0.15 Composition 5 groups 2.29±0.30 5.35±0.51 0.76±0.13 2.15±0.27 Composition 6 groups 2.37±0.24 5.50±0.49 0.79±0.05 2.11±0.20 Composition 7 groups 2.39±0.25 5.54±0.81 0.80±0.06 2.10±0.16 Composition 8 groups 2.65±0.18 6.01±0.67 0.89±0.11 1.97±0.24 Composition 9 groups 2.47±0.35 5.68±0.52 0.82±0.10 2.06±0.29 Composition 10 groups 2.28±0.25 5.33±0.64 0.76±0.08 2.16±0.18 Composition 11 groups 2.40±0.16 5.56±0.50 0.78±0.05 2.09±0.23

[0104] The reduction rate of TG, TC, and LDL-C content in each group compared with the model group and the increase rate of HDL-C content were calculated. The results are shown in Table 2.

[0105] Table 2: Reduction rate of TG, TC, LDL-C content and increase rate of HDL-C content in each group compared with the model group

[0106] test TG reduction rate / % TC reduction rate / % LDL-C reduction rate / % HDL-C increase rate / % Model Group / / / / Positive control group 28.95% 28.04% 17.82% 28.92% Composition 1 set 36.26% 29.55% 28.71% 33.13% Composition 2 sets 35.67% 29.00% 27.72% 32.53% Composition 3 groups 31.58% 25.58% 22.77% 28.31% Composition 4 groups 34.21% 27.91% 26.73% 30.72% Composition 5 groups 33.04% 26.81% 24.75% 29.52% Composition 6 groups 30.70% 24.76% 21.78% 27.11% Composition 7 groups 30.12% 24.21% 20.79% 26.51% Composition 8 groups 22.51% 17.78% 11.88% 18.67% Composition 9 groups 27.78% 22.30% 18.81% 24.10% Composition 10 groups 33.33% 27.09% 24.75% 30.12% Composition 11 groups 29.82% 23.94% 22.67% 25.90%

[0107] It can be seen from the above test results that the composition prepared by the present invention has a good auxiliary lipid-lowering effect. In the hyperlipidemia model test, the dosage was 1.2g / kg mice using the composition of the present invention for 25 days, which can reduce the TG, TC, and LDL-C levels in the mouse serum to 2.18±0.17mmol / L, 5.15±0.45mmol / L and 0.72±0.07mmol / L, and increase the HDL-C content in the mouse serum to 2.21±0.27mmol / L. And compared with the mouse serum in the model group, the reduction rates of TG, TC, and LDL-C were 36.26%, 29.55%, and 28.71%, and the increase rate of HDL-C content was 33.13%.

[0108] The test results of composition group 1 and groups 3-6 show that the process of extracting the broom extract of the present invention has a certain influence on the product's auxiliary lipid-lowering effect; and in the process of extraction with ethanol aqueous solution, when the mass concentration of ethanol aqueous solution is 65%-78%, the auxiliary lipid-lowering effect is better; and the effect of the extraction agent composed of ethyl acetate used in the present invention is better.

[0109] The test results of composition group 1 and group 7-10 show that the extract of the genus Glechoma longituba obtained by a specific process and the winged fruit oil of the present invention form a cooperative relationship, which can synergistically promote the auxiliary blood lipid lowering effect of the composition to a certain extent. When the ratio of the two is in the range of 1.5-2.5:1, the product shows better reduction of TG, TC, LDL-C content in serum and better increase of HDL-C content.

[0110] The present invention adopts Lactobacillus reuteri GMNL-263, which can make the product have better effects of reducing TG, TC, and LDL-C contents in serum and better increasing HDL-C content.

[0111] The composition prepared by the present invention, which includes wing nut oil, red yeast rice, taurine, bamboo leaf flavonoids, broom extract, Lactobacillus reuteri GMNL-263, erythritol, resistant dextrin, phospholipids and oligofructose, has good effect, uses edible substances as raw materials, can be used daily, has low impact on human body functions, and has a high efficiency in assisting the effect of lowering blood lipids.

[0112] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition containing samaras oil for assisting in lowering blood lipids, characterized in that: The raw materials for preparation are: 3-15g wingnut oil, 0.1-0.5g red yeast rice, 0.01-0.05g taurine, 0.5-2.5g bamboo leaf flavonoids, 2-4g thread-leaf broom extract, Lactobacillus reuteri GMNL-263, 0.1-0.4g erythritol, 8-50g resistant dextrin, 3-6g phospholipids and 0.2-0.8g oligofructose; The mass ratio of the samarassima oil to the safflower extract is 1.5-2.5:1; The preparation method of the broom extract comprises the following steps: Step 1: crush the dried broom to obtain powder, add water, stir at 50-70° C. for 6-15 hours, filter to obtain a filtrate and a filter residue, and freeze-dry the filtrate to obtain an intermediate material; Step 2: Mix the filter residue with an ethanol aqueous solution having a concentration of 65-78 wt%, stir at 40-55° C. for 5-12 hours, filter to obtain a filter solution, and then concentrate under reduced pressure to obtain a concentrate; Step 3: Mix the concentrate with an extractant composed of ethyl acetate and water in a mass ratio of 4-6:1, shake well, and extract at a temperature of 35-45° C. for 4-10 hours, stand to separate the layers, then pour out the water layer, and concentrate the remaining material under reduced pressure until it does not contain ethyl acetate to obtain a material; Step 4: Mix the intermediate material and the material to obtain the broom extract.

2. The composition according to claim 1, characterized in that The preparation raw materials are: 3.5-13.5g wingnut oil, 0.15-0.3g red yeast rice, 0.01-0.03g taurine, 0.8-2.3g bamboo leaf flavonoids, 2.5-3.7g thread-leaf broom extract, Lactobacillus reuteri GMNL-263, 0.1-0.3g erythritol, 10-48g resistant dextrin, 3.2-5.5g phospholipids and 0.25-0.6g oligofructose.

3. The composition according to claim 1, characterized in that In step 2, the solution is concentrated under reduced pressure to 8-15% of the mass of the filtered solution.

4. The composition according to claim 1, characterized in that In step 1, the mass ratio of powder to water is 1:20-50; In step 2, the mass ratio of the filter residue to the ethanol aqueous solution is 1:15-40; In step 3, the mass ratio of the concentrate to the extractant is 1:10-30.

5. The composition according to claim 1, characterized in that In step 3, the temperature of the reduced pressure concentration is 50-55°C and the pressure is 4.5×10 3 -7.2×10 3 Pa.

6. A method for preparing a composition for assisting in lowering blood lipids containing samaras oil according to any one of claims 1 to 5, characterized in that: The steps include: S1: weighing raw materials; stirring and mixing lecithin, samaras oil, partially resistant dextrin, and broom extract with hot water, and spray drying to obtain a first mixture; S2: mixing oligofructose, erythritol, remaining resistant dextrin, Lactobacillus reuteri GMNL-263 and water, and freeze-drying to obtain a second mixture; S3: combining red yeast rice, taurine, bamboo leaf flavonoids, the first mixture and the second mixture, and then crushing and sterilizing them to obtain the composition.

7. A composition containing samaras oil for assisting in lowering blood lipids, characterized in that: The preparation raw materials are: 3-15g wingnut oil, 0.1-0.5g red yeast rice, 0.01-0.05g taurine, 0.5-2.5g bamboo leaf flavonoids, 2-4g thread-leaf broom extract, Lactobacillus reuteri GMNL-263, 0.1-0.4g erythritol, 8-50g resistant dextrin, 3-6g phospholipids, 0.2-0.8g oligofructose and 0.1-6g powdered perilla seed oil; The mass ratio of the samarassima oil to the safflower extract is 1.5-2.5:1; The preparation method of the broom extract comprises the following steps: Step 1: crush the dried broom to obtain powder, add water, stir at 50-70° C. for 6-15 hours, filter to obtain a filtrate and a filter residue, and freeze-dry the filtrate to obtain an intermediate material; Step 2: Mix the filter residue with an ethanol aqueous solution having a concentration of 65-78 wt%, stir at 40-55° C. for 5-12 hours, filter to obtain a filter solution, and then concentrate under reduced pressure to obtain a concentrate; Step 3: Mix the concentrate with an extractant composed of ethyl acetate and water in a mass ratio of 4-6:1, shake well, and extract at a temperature of 35-45° C. for 4-10 hours, stand to separate the layers, then pour out the water layer, and concentrate the remaining material under reduced pressure until it does not contain ethyl acetate to obtain a material; Step 4: Mix the intermediate material and the material to obtain the broom extract.