Compound medicine composition and preparation method thereof and application in preparation of medicine for reducing infection rate of snail oocysts or improving hatching rate

Abstract

The present invention discloses a compound pharmaceutical composition, a preparation method thereof, and an application thereof in the preparation of a drug for reducing the infection rate of Dongfeng snail egg cysts or improving the hatching rate. According to weight parts, the compound pharmaceutical composition includes: 10 to 15 parts of a Chinese medicine compound composition and 5 to 10 parts of cecropin; according to weight parts, the Chinese medicine compound composition is made of the following raw materials, the raw materials including: 10 to 30 parts of Pulsatilla striata, 20 to 30 parts of Macleaya cordata, 10 to 20 parts of Osmanthus fragrans, 10 to 20 parts of Rhizoma Dioscoreae, 10 to 30 parts of Dictamni, 20 to 30 parts of Rhizoma Dioscoreae, 5 to 10 parts of Trichosanthes kirilowii, and 5 to 15 parts of Erigeron breviscapus. The compound pharmaceutical composition can significantly inhibit the red, white, and yellow infection of Dongfeng snail egg cysts, effectively prevent large-scale cross-infection of egg cysts, reduce the infection rate of egg cysts, and improve the hatching rate of Dongfeng snail egg cysts, thereby improving economic benefits.

Description

Technical Field

[0001] The invention belongs to the technical field of babylonica seedling cultivation, and more specifically, relates to a compound pharmaceutical composition and a preparation method thereof, and an application in the preparation of a drug for reducing the infection rate of babylonica oocysts or improving the hatching rate. Background Art

[0002] The Dongfeng conch, also known as the sea pig conch, sweet conch, and southern wind conch, belongs to the phylum Mollusca, class Gastropoda, subclass Prosobranchia, order Neogastropoda, family Buccinidae, and genus Babylonia. It is the main farmed shellfish species in my country.

[0003] Dongfeng snails have high requirements for water quality, especially during the egg sac hatching period in the seedling stage. Once the water quality is poor, it is very easy to be infected, and this infection is often difficult to detect in the early stage of egg sac hatching, which brings economic losses to the Dongfeng snail seedling work. During the seedling process, the egg sacs and water bodies are disinfected. However, if the inside of the egg sac is already infected, only the surface of the body can be disinfected, and the internal source of infection cannot be completely eliminated. In the later stage of egg sac hatching, especially in the trochophore larvae and intramembranous disc larvae, when the larvae are about to hatch, there will often be large-scale outbreaks of cross-infection, and the egg sacs will quickly show red, white, and yellow infection phenomena, which will cause the death of the larvae inside the membrane, greatly reducing the hatching rate.

[0004] At present, the prevention of red, white, and yellow infections caused by the egg cysts of the snail mainly relies on the use of chemical reagents to disinfect the egg cysts. However, in the late stage of egg cyst hatching, such disinfectants cannot be used because they will irritate the snail larvae and cause their death. At present, there is no safe and effective targeted drug, and manual selection cannot fundamentally eliminate the infection, so prevention and control work is relatively difficult.

[0005] Patent Publication No. CN101301365A discloses a Chinese and Western compound medicine composition for treating bacterial enteritis in aquatic animals, including 8-15 parts of Pulsatilla chinensis, 30-50 parts of Rhubarb, 15-25 parts of Scutellaria baicalensis, and 5-15 parts of Florfenicol. It has a good therapeutic effect on bacterial enteritis in pike, and treats both the symptoms and the root causes, thereby reducing the recurrence rate and mortality rate of aquatic animal diseases. Patent Publication No. CN105998207A discloses a Chinese medicine composition for preventing and treating bacterial diseases in aquatic animals, which includes silver leaf Croton root and Macleaya cordata, and the antibacterial effect is significantly enhanced, and the antibacterial effect is better than the existing products, and can be widely used in antibacterial agents, especially in the preparation of aquaculture antibacterial agents. However, the above-mentioned Chinese medicine composition is not for Dongfeng snails, and it is not applied to Dongfeng snail egg capsules, nor does it reflect the treatment of the red, white, and yellow infection phenomenon that Dongfeng snail egg capsules will quickly present. Therefore, finding safe, effective and low-toxic preventive and therapeutic drugs has become an urgent problem that needs to be solved. Summary of the invention

[0006] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide a compound pharmaceutical composition, which is used in combination with a traditional Chinese medicine compound composition and cecropin, and can significantly inhibit the infection phenomenon of redness, whitening and yellowing of the egg cysts of the baby snail, effectively prevent large-scale cross-infection of the egg cysts, reduce the infection rate of the egg cysts and increase the hatching rate of the baby snail egg cysts, thereby improving the economic benefits of aquaculture.

[0007] The second object of the present invention is to provide a method for preparing the compound pharmaceutical composition.

[0008] The third object of the present invention is to provide a compound pharmaceutical composition for use in the preparation of a medicine for reducing the infection rate of babylonica oocysts and / or improving the hatching rate of babylonica oocysts.

[0009] The fourth object of the present invention is to provide a method for hatching baby conch egg capsules.

[0010] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0011] A compound pharmaceutical composition comprises, by weight, 10-15 parts of a Chinese medicinal compound composition and 5-10 parts of cecropin; the Chinese medicinal compound composition is made of the following raw materials, by weight, the raw materials comprising: 10-30 parts of Pulsatilla striata, 20-30 parts of Macleanopsis pilosula, 10-20 parts of Osmanthus fragrans, 10-20 parts of Rhizoma Cyperi, 10-30 parts of Dictamni cortex, 20-30 parts of Rhizoma Cyperi, 5-10 parts of Thunbergia tenuifolia, and 5-15 parts of Erigeron breviscapus.

[0012] The present invention provides a compound pharmaceutical composition including a Chinese medicine compound composition and cecropin through research. Among them, the Chinese medicine compound composition has antibacterial, antiviral and antiparasitic effects. These ingredients can inhibit or kill pathogens such as bacteria, viruses or parasites that cause egg cyst infection, thereby reducing the infection rate of the egg cyst of the baby snail. In addition, the Chinese medicine compound composition has the function of regulating the immune system of the baby snail, and by enhancing the immune ability of the baby snail itself, it can better resist the invasion of pathogens and reduce the occurrence of egg cyst infection. Further, the Chinese medicine compound composition can improve the microecological balance of the breeding environment of the baby snail, regulate the microbial community structure in the water body, inhibit the growth of harmful microorganisms, create favorable conditions for the hatching of the egg cyst, and then improve the hatching rate. Finally, the Chinese medicine compound composition also has antioxidant and anti-inflammatory properties, which can reduce the adverse effects of environmental stress on the hatching process of the baby snail embryo, protect the normal development and hatching of the embryo, and reduce the risk of infection.

[0013] Furthermore, the present invention uses cecropin and a Chinese medicine compound composition. Cecropin has excellent antibacterial effects, can act on the cell membrane of microorganisms, change its permeability, cause physical damage to the membrane, and lead to extravasation of cell contents, thereby killing bacteria and controlling the bacterial content in the water to be lower than the safe concentration. In addition, cecropin also has an immunomodulatory effect, can participate in the immune response of the embryo, and enhance the immune function of the embryo.

[0014] The invention combines the above-mentioned Chinese medicine compound composition with cecropin to have a synergistic effect, can significantly inhibit the infection phenomenon of the red, white and yellowing of the egg cysts of the baby snails, reduce the infection rate of the egg cysts and increase the hatching rate of the egg cysts of the baby snails.

[0015] Specifically, Pulsatilla chinensis (Bunge) Regel, a perennial herb of the Ranunculaceae family and the genus Pulsatilla, has rhizomes, oval leaves and blue-purple calyx.

[0016] Specifically, Macleaya cordata (Willd.) R.Br. is an upright herb of the genus Macleaya in the family Papaveraceae, with smooth green stems; leaves are broadly ovate or nearly circular, with white powder on the back, often light red; large panicles with many flowers, terminal and axillary, with rod-shaped flower buds and sepals obovate-oblong.

[0017] Specifically, osmanthus (Osmanthus sp.), an evergreen tree or shrub of the genus Oleaceae, has thin and weak pedicels, very short filaments, and extremely fragrant flowers; the fruit is crooked, generally oval, and purple-black; the leaves are opposite, leathery, and are oval, oblong, or elliptical-lanceolate.

[0018] Specifically, Rhaponticum uniflorum (L.) DC. is a plant of the Asteraceae family and the genus Rhaponticum. The rhizome is thick and thick. The root is straight and 1-3 cm in diameter. The stem is erect, unbranched, clustered or solitary, grayish white, covered with cotton wool, with a base diameter of 0.5-1 cm, and a petiole length of 6-20 cm. The lateral lobes are 5-12 pairs, elliptical or oblanceolate.

[0019] Specifically, Dictamni bark is the root bark of Dictamni and Dictamni angustifolia, which are perennial herbs of the Rutaceae family. It is mainly produced in Liaoning, Hebei, Sichuan, Jiangsu and other places. The roots are dug up in spring and autumn, the sand and rough bark are removed, the root bark is peeled, sliced ​​and dried. It is used raw.

[0020] Specifically, the root of Patrinia rupestris (Pall.) Juss. subsp. scabra (Bunge) HJ Wang or Patrinia heterophylla Bunge. of the Patriniaceae family.

[0021] Specifically, Erycibe obtusifolia Benth. is a plant of the Convolvulaceae family, with a plant length of up to 20 meters; young branches are angular; leaves are leathery, elliptical or obovate, with a solitary or rounded apex, a cuneate base, and glabrous on both sides; axillary inflorescences have few or many flowers, terminal inflorescences are racemose, the inflorescence axis is covered with light brown short soft hands, the anthers are gradually pointed at the apex, and are nearly as long as the filaments; berries are red or yellow, ovate-elliptical.

[0022] Specifically, Erigeron chinensis is a perennial herb of the genus Erigeron in the Asteraceae family, with woody rhizomes; the leaves are mainly concentrated at the base, rosette-shaped; they survive the flowering period, and are obovate-lanceolate or broadly spoon-shaped; the stems and leaves are few, narrowly oblong-lanceolate or narrowly lanceolate; the stems and leaves are few, narrowly oblong-lanceolate or narrowly lanceolate; the involucre is hemispherical, and the bracts are linear-lanceolate; the achenes are narrowly oblong.

[0023] Preferably, the Chinese medicine compound composition comprises, by weight: 20-25 parts of Pulsatilla striata, 24-28 parts of Macleaya cordata, 15-18 parts of Osmanthus fragrans, 15-18 parts of Rhizoma Cyperi, 15-25 parts of Dictamni cortex, 22-26 parts of Rhizoma Cyperi, 6-9 parts of Caulis Trichosanthis, and 10-14 parts of Erigeron breviscapus. Further preferably, the Chinese medicine compound composition comprises: 25 parts of Pulsatilla striata, 26 parts of Macleaya cordata, 17 parts of Osmanthus fragrans, 18 parts of Rhizoma Cyperi, 20 parts of Dictamni cortex, 24 parts of Rhizoma Cyperi, 8 parts of Caulis Trichosanthis, and 12 parts of Erigeron breviscapus.

[0024] Preferably, based on weight, it comprises 12 to 14 parts of the Chinese medicine compound composition and 7 to 9 parts of cecropin.

[0025] Furthermore, the present invention claims a method for preparing a compound pharmaceutical composition, comprising the following steps:

[0026] S1. A mixture of Pulsatilla, Bo Luo Hui, Osmanthus fragrans, Lophatherum gracile, Dictamni, Toutou Hui, Ding Gong Teng, and Erigeron breviscapus was extracted by decoction, and the extract was post-treated to obtain a Chinese medicine compound composition;

[0027] S2. Mix the Chinese medicine compound composition and cecropin to obtain a compound pharmaceutical composition.

[0028] Preferably, in step S1, the post-treatment is selected from one or more of filtration, concentration and drying. More specifically, the pore size of the filtration is 180-200 mesh. More specifically, after drying, the granules are further crushed to obtain powders with a particle size of 50-100 mesh.

[0029] Preferably, in step S1, before extracting by decoction, the mixture of raw materials is crushed into powder.

[0030] Preferably, the specific operation of the decoction method is: mix a mixture of Pulsatilla striata, Macleaya rotundus, Osmanthus fragrans, Rhizoma Cyperi, Cortex Dictamni, Rhizoma Cyperi, Thunbergia paniculata, and Erigeron breviscapus with water in a mass ratio of 1:8-10, soak, decoct at least twice, mix the decocted medicinal liquids, and obtain an extract.

[0031] More preferably, the soaking time is 30 to 60 minutes.

[0032] More preferably, the decoction is divided into two times. In the first decoction, after boiling, the heat is reduced to low and the decoction is continued for 30 to 60 minutes, and then the medicinal liquid is poured out; water is added for the second decoction, and the second decoction time is 30 to 60 minutes.

[0033] Furthermore, the present invention seeks to protect the use of a compound pharmaceutical composition in the preparation of a method for reducing the infection rate of babylonica oocysts and / or improving the hatching rate of babylonica oocysts.

[0034] Specifically, the symptoms of infection with the Dongfeng snail oocyst are: the Dongfeng snail oocyst appears one or more of red, white or yellow in appearance, and the embryo inside the Dongfeng snail oocyst dissolves and dies.

[0035] Furthermore, the present invention seeks to protect a method for hatching baby cones oocysts, wherein the above-mentioned compound pharmaceutical composition is added into a breeding water body where baby cones oocysts are cultured.

[0036] Preferably, 1 to 5 grams of the compound pharmaceutical composition is added into every cubic meter of aquaculture water.

[0037] Preferably, the egg capsules of the baby snails are disinfected before the compound pharmaceutical composition is added. More specifically, the baby snail egg capsules can be disinfected with a disinfectant conventionally used in the art, such as nanosilver, and more specifically, the concentration of the nanosilver can be 3 to 6 ppm.

[0038] Preferably, during the incubation of the Dongfeng snail oocysts, the water temperature is maintained at 26-30°C, the seawater salinity is maintained at 26-30, and the pH value is maintained at 8.0-8.3.

[0039] Specifically, the babylonia is of the genus Babylonia (Babylonia), including but not limited to Babylonia areolata, Babylonia lutosa, Babylonia formosae, etc.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] The invention provides a compound pharmaceutical composition, which is used in combination with the traditional Chinese medicine compound composition and cecropin, and can significantly inhibit the infection phenomenon of redness, whiteness and yellowing of egg cysts of baby snails, effectively prevent large-area cross-infection of egg cysts, reduce the infection rate and mortality rate of larvae, increase the hatching rate of baby snail egg cysts, and improve economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is a schematic diagram of a normal embryo in a normal oocyst under a microscope in Example 5.

[0043] Figure 2 This is a schematic diagram of a normal oocyst on the first day of incubation in Example 5.

[0044] Figure 3 This is a schematic diagram of the oocyst on the 5th day of incubation in Example 5.

[0045] Figure 4 This is a schematic diagram of embryo dissolution and necrosis after oocyst infection under a microscope in Comparative Example 1.

[0046] Figure 5 This is a schematic diagram of the infected and necrotic oocysts in Comparative Example 1.

[0047] Figure 6 This is a schematic diagram of the infected yellow oocysts in Comparative Example 1.

[0048] Figure 7 This is a schematic diagram of the infected reddish oocysts in Comparative Example 1.

[0049] Figure 8 This is a schematic diagram of the infected whitish oocysts in Comparative Example 1. DETAILED DESCRIPTION

[0050] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0051] Example 1 Preparation of a compound pharmaceutical composition

[0052] (1) Weighing materials: according to weight, weigh 20 parts of Pulsatilla striata, 20 parts of Macleanium wilfordii, 10 parts of Osmanthus fragrans, 10 parts of Rhizoma Cyperi, 10 parts of Dictamni cortex, 20 parts of Rhizoma Cyperi, 8 parts of Trichosanthes kirilowii, and 10 parts of Erigeron breviscapus, wash them clean, and grind them in a grinder.

[0053] (2) Decocting the medicinal materials: Use distilled water, add 8 times the total mass of the raw materials in step (1), and soak the medicinal materials for 30 minutes so that the effective ingredients can be easily decocted. Decoction the soaked raw materials together with the soaking water in a casserole. Decoction twice. After boiling for the first time, turn to low heat and continue to decoct for 40 minutes, pour out the medicinal liquid, add water for the second decoction, and the second decoction time is 40 minutes; mix the medicinal liquids from the two decoctions to obtain an extract.

[0054] (3) Filtering the liquid medicine: The leachate is filtered through a 200-mesh stainless steel filter to remove impurities and larger particles to obtain a relatively pure liquid medicine.

[0055] (4) Concentration: Place the drug solution in a vacuum rotary evaporator, not exceeding 2 / 3 of the capacity, turn on the vacuum pump, and gradually reduce the pressure in the system to reach the required vacuum degree. Set the temperature to 50°C, the drug solution begins to evaporate, and the solvent is condensed and collected in the receiving bottle.

[0056] (5) Drying: The temperature of the freeze dryer is set at -60°C, the solvent in step (4) is placed in the freeze dryer to freeze into a solid, and then the water therein is directly sublimated into a gaseous state under vacuum conditions, and the dried solvent is taken out.

[0057] (6) Powdering: The dried solvent is crushed into a particle size of 80 meshes to obtain a Chinese medicine compound composition.

[0058] (7) 10 parts of the Chinese medicine compound composition were mixed with 10 parts of cecropin (source: Liangshan Xingzhen Biotechnology Co., Ltd.) to obtain a compound pharmaceutical composition.

[0059] Example 2 Preparation of a compound pharmaceutical composition

[0060] The difference between this embodiment and embodiment 1 is that: in terms of weight, the Chinese medicine compound composition comprises: 10 parts of Pulsatilla striata, 25 parts of Macleaya cordata, 15 parts of Osmanthus fragrans, 10 parts of Rhizoma Cyperi, 20 parts of Dictamni, 25 parts of Rhizoma Cyperi, 5 parts of Caulis Trichosanthis, and 15 parts of Erigeron breviscapus;

[0061] 15 parts of the traditional Chinese medicine compound composition are mixed with 10 parts of cecropin to obtain a compound medicine composition.

[0062] Example 3 Preparation of a compound pharmaceutical composition

[0063] The difference between this embodiment and embodiment 1 is that: in terms of weight, the Chinese medicine compound composition comprises: 25 parts of Pulsatilla striata, 26 parts of Macleaya cordata, 17 parts of Osmanthus fragrans, 18 parts of Rhizoma Cyperi, 20 parts of Dictamni, 24 parts of Rhizoma Cyperi, 8 parts of Caulis Trichosanthis, and 12 parts of Erigeron breviscapus;

[0064] 13 parts of the traditional Chinese medicine compound composition are mixed with 8 parts of cecropin to obtain a compound pharmaceutical composition.

[0065] Example 4 Preparation of a compound pharmaceutical composition

[0066] The difference between this embodiment and embodiment 1 is that: in terms of weight, the Chinese medicine compound composition comprises: 16 parts of Pulsatilla striata, 26 parts of Macleaya cordata, 16 parts of Osmanthus fragrans, 16 parts of Rhizoma Cyperi, 24 parts of Dictamni cortex, 24 parts of Rhizoma Cyperi, 8 parts of Trichosanthes kirilowii, and 12 parts of Erigeron breviscapus;

[0067] 14 parts of the traditional Chinese medicine compound composition are mixed with 9 parts of cecropin to obtain a compound pharmaceutical composition.

[0068] Example 5 Hatching of Babylonia snail oocysts

[0069] The parent snails of Babylonia square are cultured in cement pools with a temporary water depth of 40cm. Small fish, shrimps and crabs are used as baits. Feeding is done once a day, and the amount of feed is 10% of the parent snail's body weight, and it can be adjusted flexibly according to the amount of leftover bait. During the culture period, the water is run 24 hours a day, and the water volume is changed by more than 1000%. When changing the water, the leftover bait and feces are removed.

[0070] When the parent snails start to mate, the hatching pool is prepared. The hatching pool has an area of ​​12 square meters and a water depth of 1.2 meters. The seawater is pumped into the reservoir during high tide, and then enters the hatching pool in the nursery workshop after sedimentation and sand filtration. When the number of egg sacs produced by the parent snails is large, the egg sacs are removed and disinfected with 4ppm nanosilver (source: Guangdong Yuejinran Biotechnology Co., Ltd.) for 10 seconds. The egg sacs are rinsed with clean seawater and transferred to the hatching pool for incubation.

[0071] The compound pharmaceutical composition prepared in Example 1 was added to the hatching pond once a day, with an amount of 5 grams per cubic meter of aquaculture water, and the water temperature was maintained at 26-28° C., the seawater salinity was maintained at 28-29, and the pH value was maintained at 8.0-8.1. Figure 1 This is a schematic diagram of a normal embryo in a normal oocyst under a microscope in Example 5. Figure 2 This is a schematic diagram of a normal oocyst on the first day of incubation in Example 5. Figure 3 This is a schematic diagram of the egg capsule on the 5th day of incubation in Example 5. As can be seen from the above figure, the egg capsule of the baby snail has almost no infection and necrosis.

[0072] When the egg capsules of the snails are hatched, feed the planktonic larvae with shrimp pond algae. Use an 80-mesh net to filter the algae water during feeding. The feeding amount is adjusted according to the change in water color after feeding. Generally, the water becomes clear within 12 hours after feeding. Feed twice a day, once in the morning and once in the evening.

[0073] Example 6 Hatching of Babylonia snail oocysts

[0074] The difference between this embodiment and embodiment 5 is that the compound pharmaceutical composition prepared in embodiment 2 is adopted.

[0075] Example 7 Hatching of Babylonia snail oocysts

[0076] The difference between this embodiment and embodiment 5 is that the compound pharmaceutical composition prepared in embodiment 3 is adopted.

[0077] Comparative Example 1

[0078] The difference between this comparative example and Example 5 is that the compound pharmaceutical composition prepared in Example 1 is not used.

[0079] Comparative Example 2

[0080] The difference between this comparative example and Example 5 is that the compound pharmaceutical composition prepared in Example 1 contains only cecropin but no Chinese medicine compound composition.

[0081] Comparative Example 3

[0082] The difference between this comparative example and Example 5 is that the compound pharmaceutical composition prepared in Example 1 contains only the Chinese medicine compound composition but does not contain cecropin.

[0083] Comparative Example 4

[0084] The difference between this comparative example and Example 5 is that the Chinese medicine compound composition only contains Pulsatilla scutellariae.

[0085] Comparative Example 5

[0086] The difference between this comparative example and Example 5 is that the Chinese medicine compound composition only contains Macleaya cordata.

[0087] Comparative Example 6

[0088] The difference between this comparative example and Example 5 is that Bacillus subtilis is used instead of the compound pharmaceutical composition.

[0089] Test Case

[0090] The indicators observed in the above embodiments and comparative examples were tested using the following methods.

[0091] Number of early veliger larvae: The three-point sampling method is used for sampling. A 250mL beaker is used to take a cup each at the front, middle and back of the nursery pond. The average number of early veliger larvae per milliliter of water is calculated and multiplied by the water volume to obtain the number of early veliger larvae.

[0092] Total number of eggs: Randomly sample the egg sacs in the hatching pool three times, taking 100 grams each time, calculate the number of egg sacs contained in them, and find the weight of each egg sac. Then randomly take 10 egg sacs, calculate the number of embryos in each egg sac, and calculate the number of eggs contained in each egg sac. The total weight of the egg sacs divided by the weight of each egg sac and multiplied by the number of eggs contained in each egg sac will give the total number of eggs.

[0093] Infection rate: The ratio of infected oocysts to the total number of hatched oocysts. Infected oocysts are picked out and counted. The total number of hatched oocysts is the total weight of oocysts divided by the weight of each oocyst.

[0094] The calculation formula is as follows:

[0095] Hatching rate = number of early disc larvae / total number of eggs × 100%.

[0096] Infection rate = number of infected oocysts / total number of hatched oocysts × 100%.

[0097] Judgment criteria: The appearance of the infected oocysts is one or more of red, white or yellow, and the embryos inside the oocysts are dissolved and die. The test results of the embodiments and comparative examples are shown in Table 1.

[0098] Table 1

[0099] index Example 5 Example 6 Example 7 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Infection rate % 3 5 2 85 52 55 66 Hatching rate% 89 86 90 8 47 40 16 index Comparative Example 5 Comparative Example 6 Infection rate % 63 69 Hatching rate% 19 14

[0100] As can be seen from the above Examples 5 to 7, the compound pharmaceutical composition provided by the present invention can significantly inhibit the infection phenomenon of redness, whiteness and yellowing of the egg capsules of the baby snails, effectively prevent large-scale cross-infection of the egg capsules, reduce the infection rate of the baby snail larvae and increase the hatching rate of the baby snail egg capsules. The infection rate of the baby snail egg capsules is ≤5%, and the hatching rate is ≥86%; further, the infection rate of the baby snail egg capsules is ≤3%, and the hatching rate is ≥89%.

[0101] It can be seen from Example 5, Comparative Example 1, Comparative Example 2 and Comparative Example 3 that the combination of the Chinese medicine compound and cecropin has the effect of synergistically reducing the infection rate of the larvae of the baby snail and increasing the hatching rate of the egg capsules of the baby snail. Figures 3 to 8 As shown, they are schematic diagrams of embryonic dissolution and necrosis, infected necrotic oocysts, infected yellow oocysts, infected red oocysts, and infected white oocysts after oocyst infection under a microscope in Comparative Example 1. It can be seen from the figure and the above comparative example data that it is difficult to achieve the technical effect of the present invention without using the compound pharmaceutical composition, or only using the traditional Chinese medicine compound composition or only using cecropin.

[0102] It can be seen from Example 5, Comparative Example 4 and Comparative Example 5 that it is also difficult to achieve the technical effect of the present invention by only using Pulsatilla striata or Macleaya cordata which are conventionally used for bactericidal or bacteriostatic purposes in the art.

[0103] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. A compound pharmaceutical composition for reducing the infection rate of babylonica oocysts and / or increasing the hatching rate of babylonica oocysts, characterized in that: The composition is composed of the following components by weight: 10 to 15 parts of the Chinese medicine compound composition and 5 to 10 parts of cecropin; The Chinese medicine compound composition is made of the following raw materials in parts by weight, and the raw materials are composed of the following components: 10 to 30 parts of Pulsatilla striata, 20 to 30 parts of Macleanopsis pilosula, 10 to 20 parts of Osmanthus fragrans, 10 to 20 parts of Rhizoma Cyperi, 10 to 30 parts of Cortex Dictamni, 20 to 30 parts of Rhizoma Cyperi, 5 to 10 parts of Thunbergia tenuifolia, and 5 to 15 parts of Erigeron breviscapus.

2. The compound pharmaceutical composition according to claim 1, characterized in that: The raw materials are composed of the following components in parts by weight: 20-25 parts of Pulsatilla striata, 24-28 parts of Macleanium wilfordii, 15-18 parts of Osmanthus fragrans, 15-18 parts of Rhizoma Cyperi, 15-25 parts of Cortex Dictamni, 22-26 parts of Rhizoma Cyperi, 6-9 parts of Thunbergia tenuifolia, and 10-14 parts of Erigeron breviscapus.

3. The compound pharmaceutical composition according to claim 1, characterized in that: The compound medicine composition consists of the following components in parts by weight: 12 to 14 parts of the Chinese medicine compound composition and 7 to 9 parts of cecropin.

4. The method for preparing the compound pharmaceutical composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. A mixture of Pulsatilla, Bo Luo Hui, Osmanthus fragrans, Lophatherum gracile, Dictamni, Toutou Hui, Ding Gong Teng, and Erigeron breviscapus was extracted by decoction, and the extract was post-treated to obtain a Chinese medicine compound composition; S2. Mix the Chinese medicine compound composition and cecropin to obtain a compound pharmaceutical composition.

5. The preparation method according to claim 4, characterized in that: In the step S1, the post-treatment is selected from one or more of filtration, concentration and drying.

6. Use of the compound pharmaceutical composition according to any one of claims 1 to 3 in the preparation of a drug for reducing the infection rate of babylonica oocysts and / or increasing the hatching rate of babylonica oocysts.

7. A method for hatching baby conch egg capsules, characterized in that: The compound pharmaceutical composition according to any one of claims 1 to 3 is added into aquaculture water in which babylonica oocysts are cultured.

8. The incubation method according to claim 7, characterized in that: 1 to 5 grams of the compound pharmaceutical composition is added into every cubic meter of aquaculture water.

9. The incubation method according to claim 7, characterized in that: Before adding the compound pharmaceutical composition, the egg cysts of the baby snail are disinfected.

10. The incubation method according to claim 7, characterized in that: During the incubation period of Dongfeng snail egg sacs, the water temperature is maintained at 26-30℃, the seawater salinity is 26-30, and the pH value is 8.0-8.3.

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