A vertical phase-separated intestinal bionic reactor and control method thereof

Abstract

The present invention belongs to the technical field of bioreactors, and provides a vertical phase-separated intestinal bionic reactor and a control method thereof. The bionic reactor includes a tank body, a feeding device, a bionic intestinal device, a discharging device and a stirring device; the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device; the bionic intestinal device includes a bionic intestine, and the bionic intestine is a hollow tubular structure, and a stirring device is arranged in a spiral around the tank body. The present invention is based on the traditional CSTR fully mixed anaerobic reactor and process, introduces intestinal bionic technology, and designs the specific connection and execution mode of the intestine and the reactor, realizes the two-phase separation reaction of the material and the inoculum through the intestinal bionic system, has new improvements and breakthroughs in the structure and operation mode of the bioreactor, and greatly improves the degradation efficiency of cellulose materials.

Description

Technical Field

[0001] The invention belongs to the technical field of bioreactors, and in particular relates to a vertical phase-separated intestinal bionic reactor and a control method thereof. Background Art

[0002] As the global demand for controlling fossil fuel consumption and reducing CO2 emissions has become a consensus, the development of biomass energy utilization has become an important task. Crop straw is considered to be an ideal energy raw material due to its high biomass yield. Green resource processing can be achieved by anaerobic fermentation of straw microorganisms to produce methane. However, when cellulose materials such as straw are used as the main raw materials for microbial anaerobic fermentation, they are prone to float up to form a scum layer crust, which leads to low gas production efficiency. It also reduces the effective volume of the fermentation tank, so that the straw cannot be completely decomposed, seriously affecting the mass transfer, heat transfer and fluidity of the solid, liquid and gas phases in the fermentation tank, and the product accumulates and leads to inhibition of biochemical reactions. Therefore, it is urgent to construct a new biochemical reactor to reduce material crusting and increase the mass transfer effect of the material and liquid during the biochemical reaction in the reactor.

[0003] Intestinal bionics is an in vitro experimental device that constructs an animal intestinal environment in engineering practice or laboratory. This device can simulate physiological conditions such as flow mixing in the gastrointestinal tract, optimize fluid dynamics design and reduce the occurrence of blockage problems, greatly improving the flexibility and effectiveness of experimental research. The Chinese invention patent with application number CN202110696460.2 discloses a simulated intestinal high-content organic material anaerobic digestion reactor. The device uses three horizontal installation cylinders to be installed in layers in sequence to avoid blockage problems. However, it does not consider the solid-liquid-gas separation process, which may lead to sediment accumulation and decreased reaction effect. The utility model authorized patent with application number CN202122485066.2: a multifunctional fully mixed bioreactor, which reacts in a traditional fermentation tank, and there are limitations in stirring, resulting in uneven mixing of materials, decreased gas production rate, and foam and scum on the upper part of the reactor and a large amount of deposited solids at the bottom.

[0004] Therefore, there is still much room for improvement in the intestinal bionic devices currently used for microbial fermentation of cellulosic materials. Summary of the invention

[0005] The technical problem to be solved by the present invention is that the existing microbial fermentation bionic devices have defects such as sediment accumulation and uneven mixing. The shortcomings and defects mentioned in the above background technology are overcome and a vertical phase-separated intestinal bionic reactor and a reaction method thereof are provided.

[0006] In order to solve the above technical problems, the technical solution proposed by the present invention is:

[0007] A vertical phase-separated intestinal bionic reactor, the bionic reactor comprising a feeding device, a tank body, a bionic intestinal device, a discharging device and a stirring device; the feeding device is arranged at the upper end of the tank body, the discharging device is arranged at the lower end of the tank body, the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device;

[0008] The bionic intestinal device comprises one or more bionic intestinals, and the bionic intestinals are in a hollow tubular structure, and a stirring device is arranged in a spiral manner inside a tank.

[0009] The present application designs a vertical phase-separated intestinal bionic reactor. After the material is put into the feed port, it falls in the bionic intestine and reacts with the microorganisms in the reactor. During the reaction, the material is stabilized in the bionic intestine, ensuring a good solid-phase separation effect, less scum formation, and can avoid accumulation and precipitation due to contact with the inner wall of the reactor or the agitator; the material spirally falls through the intestinal structure and is operated in conjunction with the stirring device, which is beneficial to increasing the reaction time and contact area between the material and the microorganisms in the inoculated mud, thereby increasing the reaction rate. The generated biogas passes through the outer wall of the bionic intestine and enters the tank, and overflows through buoyancy, and then realizes gas phase separation, which greatly improves the reaction rate and continuous reaction efficiency.

[0010] Preferably, the tank body comprises a reactor tank body, and the inoculated mud is stored in the reactor tank body.

[0011] Under the action of mechanical stirring, the inoculated mud around the bionic intestine maintains good fluidity, which can reduce the accumulation of intermediates in the biochemical reaction, prevent the occurrence of acid inhibition, and improve the degradation efficiency; these advantages work together to effectively ensure the stability of the fermentation process of the bionic intestine reactor; on the other hand, stirring can accelerate the escape of the generated biogas and achieve the separation of the gas and solid-liquid mixed areas.

[0012] Preferably, the bionic intestine is in a spiral shape, which includes a starting section, a spiral section and an exit section;

[0013] The starting section is connected to the feeding device, and the angle between the starting section and the horizontal plane is 40-90°;

[0014] The spiral section is arranged along the inner wall of the tank, and the angle between the spiral section and the horizontal plane at any point in the falling direction is 30-60°

[0015] The outlet section is connected to the discharging device, and the angle between the outlet section and the horizontal plane is 40-90°.

[0016] The spiral section is arranged close to the inner wall of the tank and can be wound in one section, one circle or multiple circles to meet the reaction time required for the degradation of different materials according to the requirements of the height-to-diameter ratio and residence time of different bioreactors.

[0017] .The material enters the spiral section along the starting section. Due to the design of the spiral section and the pushing effect of the material in the bionic intestine, the material is pushed forward at a certain speed; at the same time, the speed can be adjusted and the retention can be carried out according to the process requirements, thereby controlling the reaction time, which is conducive to the full reaction of the material. Combined with the stirring device, the mixing degree of the material in the intestine and the liquid in the tank is further improved, the mass transfer and stirring effects are enhanced, and the gas production is increased. In addition, the hollow structure of the bionic intestine allows the generated gas to be discharged through the hollow part, avoiding the phenomenon of material floating.

[0018] More preferably, the ratio of the total height of the spiral segment in the vertical direction to the radius of the circular projection formed by the spiral segment in the horizontal direction is 2-3, and the ratio of the total height of the spiral segment in the vertical direction to the height of the tank body is 18%-42%.

[0019] Preferably, the material of the bionic intestine is at least one of metal or plastic, and the inner diameter of the bionic intestine is 1 / 10-1 / 4 of the inner diameter of the bionic reactor.

[0020] More preferably, the bionic intestine adopts a hollow design as a whole, which can increase the mixing surface area of ​​the microbial mud and the material. When a metal or plastic hollow material is used, its porosity is between 50% and 95%.

[0021] Preferably, the stirring device includes a motor, a coupling and a stirring shaft. The motor is arranged on the outside of the reactor tank body and is connected to the stirring shaft through the coupling. The stirring shaft vertically passes through the outer shell of the reactor tank body to the inside of the tank body. The stirring shaft extends below the liquid level of the inoculation mud in the reactor tank body. One or more paddles are arranged on the stirring shaft.

[0022] The motor drives the stirring shaft to rotate, which accelerates the mixing of the material and the surrounding inoculum mud, improves the heat and mass transfer rates, maintains the stability of the microbial community structure, and promotes the reaction.

[0023] Preferably, the surface of the paddle blade is arc-shaped and is connected to the stirring shaft through fastening sleeve one and fastening sleeve two. Fastening sleeve one controls the angle between the paddle blade and the horizontal plane, and fastening sleeve two controls the blade height of the paddle blade and the direction of the blade in the horizontal direction of the stirring shaft.

[0024] The direction, number and height of the paddles can be adjusted on the stirring shaft, which can adapt to intestinal structures of different lengths and angles, and can promptly stir the VFA produced during the reaction and the accumulated ammonia nitrogen solution evenly.

[0025] More preferably, the stirring shaft comprises a single propeller blade and / or multiple propeller blades.

[0026] Preferably, the feeding device comprises one or more feeding ports, the feeding port comprises a feeding conduit, the feeding conduit is in the shape of a funnel, and has an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level of the inoculated mud in the reactor tank, and is connected to the bionic intestine through a clamp, and the open funnel end is sealed;

[0027] The design of the feeding device ensures the effective sealing of the feeding port and the position below the liquid level to prevent biogas leakage, while allowing multiple feeding ports to be set according to the actual reaction tank volume and fermentation requirements; the feeding port is sealed with a metal clamp and a rubber gasket, which is opened during feeding and sealed and locked under normal circumstances to prevent biogas leakage.

[0028] Preferably, the discharging device comprises a discharging port and a connecting elbow, and the discharging port is connected to the bionic intestine via the connecting elbow;

[0029] Preferably, the bionic reactor further comprises a temperature control system, and the temperature control system comprises a water bath jacket arranged outside the reactor tank;

[0030] The water bath jacket is heated or cooled by a hot water circulation system, so that the fermentation process is carried out within the optimal temperature range, ensuring the activity of the microorganisms and the fermentation efficiency. The temperature control of the water bath jacket is precise and can be adjusted according to the fermentation needs, thereby improving the overall performance of the system.

[0031] The reactor tank is also provided with a glass window and a supporting base.

[0032] The glass window is arranged corresponding to the liquid level of the inoculated mud, so as to facilitate observation of the distribution of the fermentation liquid level and the gas production.

[0033] Under the same technical concept, the present application also provides a control method for a vertical phase-separated intestinal bionic reactor, comprising the following steps:

[0034] (1) Adding inoculum mud into the vertical phase-separated intestinal biomimetic reactor tank;

[0035] (2) feeding the material from the feeding device into the bionic intestinal device, turning on the stirring device to stir, and cultivating the inoculated mud;

[0036] (3) After the inoculation mud is cultivated, the material is added. After the reaction between the material and the inoculation mud is completed, the discharging device is opened to discharge the material and collect it.

[0037] More preferably, the inoculated mud should be sieved to remove impurities larger than 10 mm before use, and the mass ratio of VS in the inoculated mud is 0.7%-3%;

[0038] Preferably, in step (3), the organic load is set or adjusted according to the amount of material input and the material residence time.

[0039] Preferably, when the material is discharged and collected in step (3), the discharge mechanism (7) in the discharge device is opened to discharge the material, and the inoculated mud is retained in the bionic reactor for repeated use.

[0040] More preferably, the material is a substrate including cellulose material, high-solid livestock and poultry manure, and energy crops.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] (1) The present invention is based on the traditional CSTR fully mixed anaerobic reactor and process, introduces intestinal bionic technology, and designs the specific connection and control method between the intestine and the reactor. The two-phase separation reaction of the material and the inoculum is realized through the intestinal bionic system, which has made new improvements and breakthroughs in the structure and operation mode of the bioreactor;

[0043] (2) During the actual operation of the bionic intestinal device of the present invention, it is ensured that the solid material and the inoculated mud can be fully contacted while being separated and added, thereby improving the mass transfer effect and being able to discharge the generated gas in a timely manner; the present invention effectively improves the gas production efficiency of anaerobic fermentation and provides an efficient and stable anaerobic fermentation solution;

[0044] (3) By changing the layout of the blades, the locally accumulated ammonia nitrogen and VFA are evenly dispersed, so that the stirring effect can be better, the stability of the microbial community structure in the reactor is maintained, and acid inhibition is avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0046] Figure 1 It is a schematic diagram of the internal cross-sectional structure of a vertical phase-separated intestinal biomimetic reactor;

[0047] Figure 2 It is a schematic diagram of the external structure of the vertical phase-separated intestinal biomimetic reactor;

[0048] Figure 3 is a comparison chart of gas production of microbial fermentation reaction in Example 1 and Comparative Example 1;

[0049] Figure 4 is a schematic diagram of the bionic intestinal structure in Example 2;

[0050] Figure 5 Schematic diagram of the stirring shaft structure of Example 1;

[0051] Figure 6 This is a schematic diagram of the bionic intestinal structure.

[0052] Among them, 1. motor; 2. feed port; 3. bionic intestine; 4. bionic intestine tubular surface; 5. water bath jacket; 6. reactor tank; 7. discharge mechanism; 8. coupling; 9. glass window; 10. stirring shaft; 11. support base; 12. connecting elbow; 13. feed duct; 18. paddle; 21. clamp; 22. exhaust port; 23. first sampling port; 24. second sampling port; 25. third sampling port; 26. water inlet; 27. water outlet; 30. fastening sleeve one; 31. fastening sleeve two; A. feed section; B. spiral section; C: outlet section. DETAILED DESCRIPTION

[0053] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with the accompanying drawings and preferred embodiments of the present invention, but the protection scope of the present invention is not limited to the following specific embodiments.

[0054] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0055] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0056] like Figure 1 , 2 , 4, 5, among which, 1. motor; 2. feed port; 3. bionic intestine; 4. tubular surface of bionic intestine; 5. water bath jacket; 6. reactor tank; 7. discharge mechanism; 8. coupling; 9. glass window; 10. stirring shaft; 11. support base; 12. connecting elbow; 13. feed duct; 21. clamp; 22. exhaust port; 23. first sampling port; 24. second sampling port; 25. third sampling port; 26. water inlet; 27. water outlet, A. feed section; B. spiral section; C: outlet section.

[0057] The present application provides a vertical phase-separated intestinal biomimetic reactor, including a tank body, a feeding device, a discharging device, a stirring device, a biomimetic intestinal device and a temperature control device. The tank body includes a reactor tank body 6, which is installed vertically and fixed on a supporting base 11 by bolt connection. One or more feed ports 2 are arranged at the upper end of the reactor tank body 6; the feed port 2 is funnel-shaped, with an open funnel end and a sealed end, wherein the sealed end connects the feed conduit 13 to the biomimetic intestinal 3 through a clamp 21, and the biomimetic intestinal tract is located below the liquid level in the reactor tank body 6. The open funnel end is sealed with a metal clamp and a rubber gasket, which is opened during feeding and sealed and locked under normal conditions to prevent biogas leakage.

[0058] The stirring device is located at the upper end of the reactor tank body 6 and is connected and fixed to the stirring shaft 10 through a coupling 8; the stirring device includes a motor 1, a coupling 8 and a stirring shaft 10, the motor 1 is arranged at the uppermost end of the reactor tank body 6, and is connected to the stirring shaft 10 through the coupling 8, the stirring shaft 10 vertically passes through the outer shell of the reactor tank body 6 to the inside of the tank body, the stirring shaft 10 extends below the liquid level in the reactor tank body 6, and one or more paddles 29 are arranged on the stirring shaft 10.

[0059] The stirring shaft 10 is a built-in design, with a spiral blade layout. The blade surface of the paddle 18 is arc-shaped and fixed on the stirring shaft 10. The paddle 18 is distributed in parallel with the intestine and is connected to the stirring shaft 10 through a fastening sleeve 1 30 and a fastening sleeve 2 31. The fastening sleeve 1 30 controls the angle between the blade 29 and the horizontal plane, and the fastening sleeve 2 31 controls the blade height of the paddle 29 and the direction of the blade in the horizontal direction of the stirring shaft 10. The paddles are distributed in the spiral section of the bionic intestine, which can effectively mix the materials and promote the reaction. The propeller blade length is sufficient to avoid collision with the outer wall of the intestine, and the blade surface is arc-shaped, which can fully disturb the fermented liquid and ensure the stirring shear force without damaging the microorganisms. The blade can adjust the angle through the fastening sleeve to ensure that it is parallel to the direction of the curved section of the intestine, further improving the stirring effect; the rotation speed of the stirrer can be adjusted to meet the stirring requirements under different reaction conditions. The motor drives the stirring shaft to rotate, accelerating the mixing of the inoculated mud around the bionic intestine, increasing the heat and mass transfer rates, maintaining the stability of the microbial community structure, and promoting the reaction. According to the reaction conditions at different stages, the stable performance of anaerobic fermentation to produce methane can be maintained.

[0060] The bionic intestinal device includes a bionic intestinal 3, which is a mesh hollow tube structure, and a stirring device is arranged in a spiral inside the tank body, which is arranged as one or more, close to the inner wall of the reactor tank body 6, and connected to the feeding device and the discharging device through a clamp respectively; the bionic intestinal tubular surface 4 is a curved surface, which can increase the specific surface area in contact with the fermentation liquid during the reaction process; the intestinal pores can meet the requirement of less than half of the volume of the added material. The entrance adopts an open trumpet shape to ensure smooth entry of the material.

[0061] The reaction area of ​​the bionic intestinal device is as follows Figure 6 As shown, the design simulates the biological intestine and is spirally distributed, and the shape includes a starting section A, a spiral section B and an exit section C;

[0062] The starting section A is connected to the feeding device, and the angle with the horizontal plane is 40-90°;

[0063] The spiral section B is set along the inner wall of the tank, and the angle between any part of the spiral section and the horizontal plane in the falling direction is 30-60°

[0064] The outlet section C is connected to the discharging device, and the angle between the outlet section C and the horizontal plane is 40-90°.

[0065] The spiral section B is arranged close to the inner wall of the tank and can be wound in one section, one circle or multiple circles to meet the reaction time required for the degradation of different materials according to the requirements of the height-to-diameter ratio and residence time of different bioreactors.

[0066] The material enters the spiral section along the starting section. Due to the design of the spiral section, the material can fall at a certain speed to avoid blockage and jamming; it can also fall slowly to control the reaction time, which is more conducive to the full reaction of the material. In combination with the stirring device, the mixing degree of the material in the intestine and the liquid in the tank is further improved, the mass transfer and stirring effects are improved, and the gas production is increased. At the same time, due to the hollow structure of the bionic intestine, the generated gas is discharged from the hollow position to avoid the phenomenon of pushing the material up.

[0067] The relationship between the bionic intestinal coil diameter, number of turns and angle and the tank height-to-diameter ratio is:

[0068]

[0069] Where h is the height of the tank (vertical height of the bionic intestine), n is the number of spiral turns, θ is the spiral angle, which is the angle between the spiral segment and the horizontal plane; d is the diameter of the bionic intestine, and D is the spiral diameter of the pipeline inside the cylinder.

[0070] The ratio of the total height of the spiral segment in the vertical direction to the tank diameter is within (1.7~4):1. When the ratio is 4:1, it means that the bionic intestinal spiral segment is higher, which means more spiral turns, which can be used to degrade difficult-to-degrade materials such as straw, increase the contact time in the intestine, and make it fully degraded; when the ratio is 1.7:1, it means that the bionic intestinal spiral segment is shorter, which means fewer spiral turns, which can be used to degrade easily degradable materials such as sewage, livestock and poultry manure, etc., to ensure its degradation efficiency while improving the reaction efficiency.

[0071] In the tank, the material in the intestine is only distributed in the intestine and cannot be directly dispersed in the external liquid, preventing the material from floating on the surface of the reaction liquid and inhibiting the overflow of the gas produced by the material reaction, which in turn causes the material to block the exhaust hole and inhibit gas production, causing the system to become "instable".

[0072] The cross-sectional diameter of the bionic intestine 3 tubular structure is 20-40 mm, and the wall thickness is 1-2 mm.

[0073] The bionic intestine 3 is made of at least one of metal and plastic.

[0074] A water bath jacket 5 is provided outside the reaction tank body to maintain a stable temperature inside the reaction tank. The water bath jacket 5 is heated or cooled by a hot water circulation system. Hot water is introduced from the water inlet 26 and discharged from the water outlet 27, so that the fermentation process is carried out within the optimal temperature range to ensure the activity of the microorganisms and the fermentation efficiency. The temperature control of the water bath jacket is accurate and can be adjusted according to the fermentation requirements, thereby improving the overall performance of the system. A glass window 9 is also provided on the reactor tank body 6 to observe the internal conditions of the reactor.

[0075] The discharge device is located at the lower end of the solid phase separation of the reaction tank, including a discharge mechanism 7 and a connecting elbow 12. The discharge mechanism 7 is connected to the bionic intestine 3 through the connecting elbow 12. During discharge, the material is discharged and the microbial mud continues to remain in the fermentation tank, ensuring efficient biogas production while maintaining the stability of the fermentation system.

[0076] The upper end of the reactor tank body 6 is provided with an exhaust port 22 for exhausting the gas generated during the reaction process, and is also provided with a first sampling port 23, a second sampling port 24, and a third sampling port 25 for collecting samples.

[0077] The inoculated mud is stored in the reactor tank 6. During the reaction, the solid phase and liquid phase separation are located in the reaction tank, the gas phase separation is located on the bionic intestine, and the gas phase separation reaction area is arranged and distributed along the hollow part of the bionic intestine.

[0078] Embodiment 1:

[0079] This embodiment adopts Figure 1 The vertical phase-separated intestinal bionic reactor shown is used for microbial reaction. The bionic intestine 3 adopts a hollow mesh stainless steel structure, and its cross-sectional diameter is 1 / 10-1 / 4 of the inner diameter of the bionic reactor. The porosity of the surface of the bionic intestine 3 is 50%-95%.

[0080] The inoculum used in this example was taken from the discharge port of a restaurant waste treatment plant. The anaerobic digestion temperature of the restaurant waste treatment plant was maintained at 55°C, and a water bath was used for temperature cycling during the experiment. The inoculum was left for 2 days without adding any substance to eliminate background gas.

[0081] In this example, Miscanthus materials were collected on a farm and naturally air-dried at room temperature for 30 days. Subsequently, the Miscanthus stems were crushed to a particle size suitable for passing through a 20-mesh screen to promote particle size reduction. At the same time, the Miscanthus leaves were mechanically shredded to reduce their physical size to a diameter of about 1-1.5 cm. All samples were carefully packaged and stored at 4°C before use.

[0082] The reaction specifically comprises the following steps:

[0083] (1) Pretreatment of inoculum mud: Add inoculum mud into the tank of the vertical phase-separated intestinal biomimetic reactor; before use, the inoculum mud should be sieved to remove impurities larger than 10 mm, and the VS ratio (mass ratio) in the inoculum mud should be 0.7%-2%;

[0084] (2) Start-up phase: 0.2-0.5gVSL -1 d -1 The organic load of the material is put into the bionic intestinal device from the feeding device, and the stirring device is turned on for stirring. The material falls along the bionic intestinal tract and is fully mixed with the microorganisms in the inoculated mud, and gas is started to be produced, thereby increasing the organic load and enriching the advantageous organic load rate of the fermentation material;

[0085] (3) After the inoculation sludge culture is completed, the organic load is increased by 0.5-1.0gVSL each time -1 d -1 , the material is put in for continuous reaction, the material falls along the bionic intestinal device, stays at the connection between the bionic intestinal device and the discharge device, and reacts with the inoculated mud. The residence time is 8 days. After the reaction is completed, the discharge device is opened to collect the reacted material.

[0086] During the experiment, no agglomeration or floating, aggregation or clogging of the reaction materials was observed, and the reaction materials remained evenly distributed and maintained a stable flow. After the reaction was completed, it was observed that the reaction materials were evenly mixed inside, and there was no incomplete reaction.

[0087] The gas volume received during the reaction is measured using a gas collection device. The test results are as follows: Figure 3 As shown in the middle curve. It can be seen from the figure that there is no obvious difference between the two in the early stage (within 40 days) when the materials are just added. As the reaction proceeds and the materials are gradually added, the advantages of the bionic intestine begin to emerge, and the gas production begins to increase compared with the tank without bionic intestine. At about 120 days, the gas production is about 2-3 times that of the tank without bionic intestine. And during the whole fermentation process, the gas production of the bionic intestine is always higher than that of the tank without bionic intestine. It is precisely because of the many advantages of the bionic intestine mentioned above that its gas production is much higher than that of the tank without bionic intestine.

[0088] The degradation of lignocellulose in the fermented material was detected by a semi-automatic cellulose analyzer and a fiber detergent method. The test results are shown in Table 1: The detection method includes: sample defatting: accurately weigh 0.5 g of fermentation material into a 150 mL conical flask, add 30 mL of a mixture of ethanol and ether (volume ratio of 15:1), place it in an ultrasonic cleaner and ultrasonicate it at room temperature for 1 hour. After taking it out, transfer the sample to a G2 glass core crucible containing 2 g of quartz sand for suction filtration, and wash the conical flask and residue with ethanol, and then wash with 5 mL of acetone.

[0089] Acid detergent fiber determination: Add about 100mL 2mol / L hydrochloric acid detergent to the degreased sample in the hot extraction unit, add 3 to 5 drops of n-octanol, quickly heat to boiling, and adjust the power to maintain a slightly boiling state for 1h. After the heating is completed, filter immediately and rinse the tube wall and residue with water above 90°C until the filtrate is free of foam. Place the hot-extracted sample and crucible on the cold extraction unit of the fiber analyzer, rinse the residue with acetone 3 times, and ensure that the residue is fully mixed with acetone until the filtrate is colorless. After drying, put the crucible and residue in a (105±2)℃ oven and dry for 3 to 4h to constant weight and then weigh. Place the crucible and residue in a muffle furnace and weigh after ash treatment at (510±10)℃ for 3h. The cellulose degradation rate data obtained under the above conditions are shown in Table 1.

[0090] The lignocellulose components of the Miscanthus material before treatment were as follows: hemicellulose 30.87%, cellulose 39.40%, lignin 10.29% by mass

[0091] The lignocellulose components in the fermented material are as follows: hemicellulose 8.37%, cellulose 7.57%, lignin 3.01% by mass

[0092] Table 1

[0093]

[0094] Lignocellulose is composed of lignin, cellulose and hemicellulose. The degradation rate of lignocellulose is one of the indicators that characterize the biodegradation characteristics of biomass after the same pretreatment and the same reaction time. The key to improving methane yield is to increase the degradation rate of lignocellulose. During the fermentation process, it is mainly for the reactor efficiency to accelerate the destruction of the chemical bonds connecting hemicellulose, cellulose and lignin. It can be seen from the data in the table that the degradation rate of lignocellulose in the material after the microbial reaction of the vertical phase-separated intestinal biomimetic reactor of this application is significantly improved, indicating that the fermentation efficiency is also significantly improved.

[0095] Embodiment 2:

[0096] This embodiment uses a vertical phase-separated intestinal biomimetic reactor for microbial reaction. The only difference from Example 1 is that the biomimetic intestine 3 in this embodiment uses Figure 4 The hollow stainless steel structure shown has a pore size range of 20-40mm, a wall thickness of 1-2mm, and a pore diameter range of 10-15mm. Due to the change in the specific shape, this bionic intestine can be used for different types of materials, thereby improving the versatility and applicability of the bionic intestine anaerobic biomass reactor.

[0097] The material used in this application is corn stalks, which are dried for one month and then broken and sieved into 2-3 cm blocks for testing.

[0098] The reaction specifically comprises the following steps:

[0099] (1) Pretreatment of materials: Add inoculum mud into the tank of the vertical phase-separated intestinal biomimetic reactor; before use, the inoculum mud should be sieved to remove impurities larger than 10 mm, and the VS ratio (mass ratio) in the inoculum mud should be 0.7%-2%;

[0100] (2) 0.5 g VSL per day -1 d -1 The organic load of the material is put into the bionic intestinal device from the feeding device, and the stirring device is turned on for stirring. The material falls along the bionic intestinal tract and is fully mixed with the microorganisms in the inoculated mud, and gas production begins, thereby increasing the organic load and enriching the dominant microorganisms suitable for the fermentation material;

[0101] (3) After the inoculation sludge culture is completed, the organic load is increased by 0.5 g VSL each time -1 d -1 , put in materials for continuous reaction, and when the materials fall to the connection between the bionic intestine device and the discharging device until the reaction is completed, open the discharging device and collect the reacted materials.

[0102] Comparative Example 1:

[0103] The present invention adopts a conventional microbial reactor, and the rest of the conventional microbial reactor is consistent with Example 1, the only difference being that the bionic intestinal structure therein is removed.

[0104] This comparative example compares a conventional bioreactor with the vertical phase-separated intestinal biomimetic reactor of Example 1.

[0105] In this comparative example, an anaerobic fermentation test was conducted. The added materials and the inoculum mud used were consistent with those in Example 1. Mechanical stirring was used to uniformly mix the materials and the inoculum mud in the reaction device, and the materials were discharged after the reaction.

[0106] The degradation of lignocellulose in the fermented material was detected, and the detection data are shown in Table 1.

[0107] The gas volume received during the reaction is measured using a gas collection device. The test results are as follows: Figure 3 As shown in curve B.

Claims

1. A vertical phase-separated intestinal biomimetic reactor, characterized in that: The bionic reactor comprises a feeding device, a tank body, a bionic intestinal device, a discharging device and a stirring device; the bionic intestinal device and the stirring device are arranged inside the tank body, one end of the bionic intestinal device is connected to the feeding device, and the other end is connected to the discharging device; The bionic intestinal device comprises one or more bionic intestinals (3), wherein the bionic intestinals (3) are in the form of a hollow tube structure and a stirring device is arranged spirally around the intestinal tract; The bionic intestine (3) is in a spiral shape, and the spiral shape includes a starting section, a spiral section, and an exit section; The starting section is connected to the feeding device, and the angle between the starting section and the horizontal plane is 40-90°; The spiral section is arranged along the inner wall of the tank, and the angle between the spiral section and the horizontal plane at any point in the falling direction is 30-60°; The outlet section is connected to the discharging device, and the angle between the outlet section and the horizontal plane is 40-90°; The tank body comprises a reactor tank body (6); The stirring device comprises a motor (1), a coupling (8) and a stirring shaft (10); the motor (1) is arranged outside the reactor tank body (6) and connected to the stirring shaft (10) via the coupling (8); the stirring shaft (10) vertically passes through the outer shell of the reactor tank body (6) to the interior of the tank body; The feeding device comprises one or more feeding ports (2), wherein the feeding port (2) is in the shape of a funnel, and has an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level of the reactor tank (6) via a feeding conduit (13), and is connected to the bionic intestine (3) via a clamp (21), and the open funnel end is sealed.

2. The bionic reactor according to claim 1, characterized in that: Inoculum mud is stored in the reactor tank (6).

3. The bionic reactor according to claim 2, characterized in that: The material of the bionic intestine (3) is at least one of metal or plastic, and the inner diameter of the bionic intestine (3) is 1 / 10 to 1 / 4 of the inner diameter of the bionic reactor.

4. The bionic reactor according to claim 2, characterized in that: The stirring shaft (10) extends below the liquid level of the inoculum mud in the reactor tank (6), and one or more blades (29) are provided on the stirring shaft (10).

5. The bionic reactor according to claim 2, characterized in that: The blade of the paddle (29) is fan-shaped and is connected to the stirring shaft (10) via a first fastening sleeve (30) and a second fastening sleeve (31). The first fastening sleeve (30) controls the angle between the blade of the paddle (29) and the horizontal plane, and the second fastening sleeve (31) controls the blade height of the paddle (29) and the orientation of the blade in the horizontal direction of the stirring shaft (10).

6. The bionic reactor according to claim 2, characterized in that: The feeding device comprises one or more feeding ports (2), wherein the feeding port (2) is in the shape of a funnel, and has an open funnel end and a sealed end, wherein the sealed end is connected to below the liquid level of the inoculated mud in the reactor tank (6) through a feeding conduit (13), and is connected to the bionic intestine (3) through a clamp (21), and the open funnel end is sealed; The discharging device comprises a discharging mechanism (7) and a connecting elbow (12); the discharging mechanism (7) is connected to the bionic intestine (3) via the connecting elbow (12); The bionic reactor further comprises a temperature control system, the temperature control system comprising a water bath jacket (5) arranged outside the reactor tank body (6); the reactor tank body (6) is also provided with a glass window (9) and a support base (11).

7. A control method for a vertical phase-separated intestinal biomimetic reactor as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: (1) Adding inoculum mud into the vertical phase-separated intestinal biomimetic reactor tank; (2) feeding the material from the feeding device into the bionic intestinal device, turning on the stirring device to stir, and cultivating the inoculated mud; (3) After the inoculation mud is cultivated, the input material slides down the bionic intestinal device and stays in the bionic intestinal device, reacts with the inoculation mud during the sliding and staying period, and after the reaction is completed, the discharging device is opened to discharge the material and collect it.

8. The reaction control method according to claim 7, characterized in that: In step (3), the organic load is set or adjusted according to the amount of material input and the material residence time.

9. The reaction control method according to claim 7, characterized in that: When the material is discharged and collected in step (3), the discharge mechanism (7) in the discharge device is opened to discharge the material, and the inoculated mud is retained in the bionic reactor for repeated use.

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