New use of processed Atractylodes macrocephala Koidz. in the preparation of products for preventing and / or treating obesity

Abstract

The present invention belongs to the technical field of traditional Chinese medicine, and relates to a new use of processed Atractylodes macrocephala Koidz. in the preparation of products for preventing and / or treating obesity. The preparation method of the processed Atractylodes macrocephala Koidz. is as follows: Weigh Fructus Aurantii Immaturus, add water with a volume 6 - 16 times that of Fructus Aurantii Immaturus, soak for 0 - 2 h, boil for 0.5 - 3 h, filter, repeat the extraction of the medicinal residues 1 - 2 times, filter, combine the filtrates, concentrate to obtain Fructus Aurantii Immaturus juice; Weigh raw Atractylodes macrocephala Koidz., add Fructus Aurantii Immaturus juice, soak for 12 - 36 h, stir-fry at 100 - 150 °C for 4 - 12 min, and bake at 30 - 70 °C for 24 - 72 h to obtain the product. The present invention for the first time proves through animal experiments that the processed Atractylodes macrocephala Koidz. can significantly improve diet-induced obesity and can be used in the preparation of products for preventing and / or treating obesity.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and relates to a new use of processed Atractylodes macrocephala Koidz. in the preparation of products for preventing and / or treating obesity. Background Art

[0002] With the improvement of people's living standards and the change of lifestyle, the number of obese people is increasing. Obesity not only affects people's external image, but is also closely related to a series of diseases such as diabetes, atherosclerosis, and hypertension. The causes of obesity are mainly related to genetics, diet and living habits, drugs, and physiological factors.

[0003] In recent years, with the obvious side effects of Western medicines emerging continuously, the research reports on traditional Chinese medicine in the field of weight loss have gradually increased.

[0004] Aurantii Fructus Immaturus and Atractylodes macrocephala Koidz. are classic herb pairs in the formula for strengthening the spleen and regulating qi in traditional Chinese medicine clinics, and are commonly used in the clinical treatment of gastrointestinal diseases. In addition to being used in combination with Atractylodes macrocephala Koidz. as a medicinal flavor, Aurantii Fructus Immaturus can also be used to process Atractylodes macrocephala Koidz. The applicant previously studied the processed products of Atractylodes macrocephala Koidz. processed with Aurantii Fructus Immaturus. The prior application publication numbers are CN107115374A, CN106924389A, and CN106822210B, and it was found that the obtained processed Atractylodes macrocephala Koidz. products have good spleen-strengthening effects and can alleviate the dryness of Atractylodes macrocephala Koidz.

[0005] Based on the applicant's new discovery of the use of processed Atractylodes macrocephala Koidz., the present invention is specifically proposed. Summary of the Invention

[0006] The purpose of the present invention is to provide a new use of processed Atractylodes macrocephala Koidz. in the preparation of products for preventing and / or treating obesity, so as to provide ideas for developing new products for preventing and / or treating obesity.

[0007] The above object of the present invention is achieved by the following technical solutions:

[0008] A new use of processed Atractylodes macrocephala Koidz. in the preparation of products for preventing and / or treating obesity, wherein the preparation method of the processed Atractylodes macrocephala Koidz. is as follows: weigh Aurantii Fructus Immaturus, add 6 - 16 times the volume of water, soak for 0 - 2.0 h, boil for 0.5 - 3 h, filter, repeat the extraction of the medicinal residues 1 - 2 times, filter, combine the filtrates, concentrate to obtain Aurantii Fructus Immaturus juice; weigh raw Atractylodes macrocephala Koidz., add Aurantii Fructus Immaturus juice, soak for 12 - 36 h, stir-fry at 100 - 150 °C for 4 - 12 min, and then bake at 30 - 70 °C for 24 - 72 h to obtain.

[0009] Preferably, the preparation method of the processed Atractylodes macrocephala Koidz. product is as follows: Weigh Fructus Aurantii Immaturus, add 14.3 times the volume of water, soak for 0.5 h, boil for 1.5 h, filter, add 12 times the volume of water to the residue, boil for 1.5 h, filter, combine the filtrates, concentrate to obtain Fructus Aurantii Immaturus juice; Weigh raw Atractylodes macrocephala Koidz., add Fructus Aurantii Immaturus juice, soak for 24 h, stir-fry at 110 °C for 6 min and then bake at 40 °C for 24 h to obtain the product.

[0010] Preferably, the weight of Fructus Aurantii Immaturus corresponding to the Fructus Aurantii Immaturus juice is 0.1 - 10% of the weight of raw Atractylodes macrocephala Koidz.

[0011] Preferably, the weight of Fructus Aurantii Immaturus corresponding to the Fructus Aurantii Immaturus juice is 1 - 10% of the weight of raw Atractylodes macrocephala Koidz.

[0012] Preferably, the weight of Fructus Aurantii Immaturus corresponding to the Fructus Aurantii Immaturus juice is 10% of the weight of raw Atractylodes macrocephala Koidz.

[0013] Preferably, the product is a drug, a health product, a beverage or a food.

[0014] Preferably, the processed Atractylodes macrocephala Koidz. product constitutes the drug as a single component or in combination with other pharmaceutically acceptable traditional Chinese medicines having a weight loss effect.

[0015] Preferably, the drug further comprises a pharmaceutically acceptable carrier and / or excipient.

[0016] Preferably, the dosage form of the drug includes one or more of decoction, herbal soup, tablets, granules and capsules.

[0017] Preferably, the decoction is a water decoction.

[0018] Preferably, the preparation method of the water decoction comprises the following steps: pulverize the processed Atractylodes macrocephala Koidz. product, add 6 - 10 times the volume of water, soak for 20 - 40 min, reflux extract 1 - 3 times, each time for 1 - 2 h, filter, collect the filtrate, and concentrate at 60 °C to obtain the product.

[0019] Preferably, the preparation method of the water decoction comprises the following steps: pulverize the processed Atractylodes macrocephala Koidz. product, add 8 times the volume of water, soak for 30 min, reflux extract 8 times, each time for 1.5 h, filter, collect the filtrate, and concentrate to 0.5 g / mL at 60 °C to obtain the product.

[0020] Preferably, the obesity is diet-induced obesity.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention for the first time proves through animal experiments that the processed Atractylodes macrocephala Koidz. product can significantly improve diet-induced obesity and can be used to prepare products for preventing and / or treating obesity. Description of the Drawings

[0023] Figure 1 A in it is the body weight of male rats in each group 1 day before drug administration. In the figure: **P<0.01 indicates extremely significant difference compared with the model group, and ns indicates no significant difference;

[0024] B is the body weight change rate of male rats in each group on the 7th day after drug administration. In the figure, **P<0.01 indicates extremely significant difference compared with the model group, and *P<0.05 indicates significant difference compared with the model group;

[0025] C is the body weight change rate of male rats in each group on the 14th day after drug administration. In the figure, **P<0.01 indicates extremely significant difference compared with the model group;

[0026] D is the body weight change rate of male rats in each group on the 23rd day after drug administration. In the figure, **P<0.01 indicates extremely significant difference compared with the model group. Specific implementation mode

[0027] The present invention will be further described below by way of specific examples. The medicinal materials used in the examples and comparative examples of the present invention are all the medicinal materials included in the Pharmacopoeia of 2020 edition. After identification, all indicators meet the regulations.

[0028] Example 1

[0029] Weigh 100 g of Fructus Aurantii Immaturus, soak it in 1430 mL of water for 30 min, boil it for 1.5 h, filter it. Add 1200 mL of water to the medicinal residues and boil for 1.5 h, then filter. Combine the filtrates and concentrate to 500 mL to obtain Fructus Aurantii Immaturus juice. Among them, 1 mL of Fructus Aurantii Immaturus juice is equivalent to 0.2 g of Fructus Aurantii Immaturus;

[0030] Weigh Rhizoma Atractylodis Macrocephalae, add 50 mL of Fructus Aurantii Immaturus juice, and the corresponding dosage of Fructus Aurantii Immaturus is 10% of that of Rhizoma Atractylodis Macrocephalae. Soak it for 24 h and turn it over from time to time until the juice is exhausted and the medicine is penetrated. Stir-fry it at 110 °C for 6 min, take it out, and bake it in an oven at 40 °C for 24 h to obtain the processed product of Rhizoma Atractylodis Macrocephalae;

[0031] Crush the processed product of Rhizoma Atractylodis Macrocephalae, soak it in 8 times the volume of water for 30 min, reflux and extract 8 times, each time for 1.5 h, filter it, collect the filtrate, and concentrate it to 0.5 g / mL at 60 °C to obtain the decoction.

[0032] Example 2

[0033] Weigh 100 g of Fructus Aurantii Immaturus, soak it in 1430 mL of water for 30 min, boil it for 1.5 h, filter it. Add 1200 mL of water to the medicinal residues and boil for 1.5 h, then filter. Combine the filtrates and concentrate to 500 mL to obtain Fructus Aurantii Immaturus juice. Among them, 1 mL of Fructus Aurantii Immaturus juice is equivalent to 0.2 g of Fructus Aurantii Immaturus;

[0034] Weigh the raw Atractylodes macrocephala Koidz., add 50 mL of Fructus Aurantii Immaturus juice, and the corresponding dosage of Fructus Aurantii Immaturus is 1% of the raw Atractylodes macrocephala Koidz.. Soak for 24 h, turning it over from time to time until the juice is exhausted and the medicine is thoroughly penetrated. Stir-fry at 110 °C for 6 min, take it out, and bake it in an oven at 40 °C for 24 h to obtain the processed product of Atractylodes macrocephala Koidz..

[0035] Crush the processed product of Atractylodes macrocephala Koidz., add water at 8 times the volume for soaking for 30 min, reflux and extract 8 times, 1.5 h each time, filter, collect the filtrate, and concentrate it to 0.5 g / mL at 60 °C to obtain the decoction.

[0036] Example 3

[0037] Weigh 100 g of Fructus Aurantii Immaturus, add 1430 mL of water for soaking for 30 min, boil for 1.5 h, filter, add 1200 mL of water to the medicinal residues and boil for 1.5 h, filter, combine the filtrates, and concentrate to 500 mL to obtain Fructus Aurantii Immaturus juice, where 1 mL of Fructus Aurantii Immaturus juice is equivalent to 0.2 g of Fructus Aurantii Immaturus.

[0038] Weigh the raw Atractylodes macrocephala Koidz., add 50 mL of Fructus Aurantii Immaturus juice, and the corresponding dosage of Fructus Aurantii Immaturus is 5% of the raw Atractylodes macrocephala Koidz.. Soak for 24 h, turning it over from time to time until the juice is exhausted and the medicine is thoroughly penetrated. Stir-fry at 110 °C for 6 min, take it out, and bake it in an oven at 40 °C for 24 h to obtain the processed product of Atractylodes macrocephala Koidz..

[0039] Crush the processed product of Atractylodes macrocephala Koidz., add water at 8 times the volume for soaking for 30 min, reflux and extract 8 times, 1.5 h each time, filter, collect the filtrate, and concentrate it to 0.5 g / mL at 60 °C to obtain the decoction.

[0040] Example 4

[0041] Weigh 100 g of Fructus Aurantii Immaturus, add 1430 mL of water for soaking for 30 min, boil for 1.5 h, filter, add 1200 mL of water to the medicinal residues and boil for 1.5 h, filter, combine the filtrates, and concentrate to 500 mL to obtain Fructus Aurantii Immaturus juice, where 1 mL of Fructus Aurantii Immaturus juice is equivalent to 0.2 g of Fructus Aurantii Immaturus.

[0042] Weigh the raw Atractylodes macrocephala Koidz., add 50 mL of Fructus Aurantii Immaturus juice, and the corresponding dosage of Fructus Aurantii Immaturus is 0.1% of the raw Atractylodes macrocephala Koidz.. Soak for 24 h, turning it over from time to time until the juice is exhausted and the medicine is thoroughly penetrated. Stir-fry at 110 °C for 6 min, take it out, and bake it in an oven at 40 °C for 24 h to obtain the processed product of Atractylodes macrocephala Koidz..

[0043] Crush the processed product of Atractylodes macrocephala Koidz., add water at 8 times the volume for soaking for 30 min, reflux and extract 8 times, 1.5 h each time, filter, collect the filtrate, and concentrate it to 0.5 g / mL at 60 °C to obtain the decoction.

[0044] Control Example 1

[0045] Weigh 1400 g of Fructus Aurantii Immaturus and Atractylodis Macrocephalae with a mass ratio of 2:1, add 1400 mL of water, soak for 30 min, decoct for 40 min, filter, add 8400 mL of water to the residue and continue to decoct for 40 min, filter, combine the filtrates, and concentrate to 0.5 g / mL at 60 °C to obtain the water decoction.

[0046] Comparative Example 2

[0047] Weigh 100 g of Fructus Aurantii Immaturus, add 1430 mL of water, soak for 30 min, boil for 1.5 h, filter, add 1200 mL of water to the residue and boil for 1.5 h, filter, combine the filtrates, and concentrate to 500 mL to obtain the Fructus Aurantii Immaturus juice. Among them, 1 mL of the Fructus Aurantii Immaturus juice is equivalent to 0.2 g of Fructus Aurantii Immaturus.

[0048] Weigh raw Atractylodis Macrocephalae, add 50 mL of the Fructus Aurantii Immaturus juice, and the corresponding dosage of Fructus Aurantii Immaturus is 10 times that of raw Atractylodis Macrocephalae. Soak for 24 h and turn it over from time to time until the juice is exhausted and the medicine is penetrated. Stir-fry at 110 °C for 6 min, take out, and bake in an oven at 40 °C for 24 h to obtain the processed product of Atractylodis Macrocephalae.

[0049] Crush the processed product of Atractylodis Macrocephalae, add 8 times the volume of water, soak for 30 min, reflux extract 8 times, 1.5 h each time, filter, collect the filtrate, and concentrate to 0.5 g / mL at 60 °C to obtain the water decoction.

[0050] Comparative Example 3

[0051] Weigh 10 g of Fructus Aurantii Immaturus and 100 g of raw Atractylodis Macrocephalae, add 8 times the volume of water, soak for 30 min, reflux extract 8 times, 1.5 h each time, filter, collect the filtrate, and concentrate to 0.5 g / mL at 60 °C to obtain the water decoction.

[0052] Experimental Example

[0053] I. Materials and Methods

[0054] 1. Experimental materials

[0055] 1.1 Materials and reagents

[0056] The medicinal materials required for the experiment include: Orlistat, Fructus Aurantii Immaturus, and Atractylodis Macrocephalae.

[0057] Other materials: Ultra-pure water (sterilized); syringe; gavage needle; rat fixator, etc.

[0058] 1.2 Experimental instruments

[0059] Electronic scale; autoclave; electric heating mantle; rotary evaporator; electrothermal blast drying oven; ultra-pure water machine.

[0060] 1.3 Experimental drugs

[0061] Test drugs: The water decoctions of Examples 1 - 4 and Comparative Examples 1 - 3.

[0062] Positive drug: Four capsules of orlistat (trade name: Orlistat Capsules, specification: 0.12 g, manufacturer: Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd.) were dissolved in 51 mL of physiological saline.

[0063] 1.4 Experimental animals

[0064] 54 male SD rats weighing over 500 g (provided by the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine).

[0065] 2. Experimental methods

[0066] During the experiment, the animals were all raised in the same environment, maintaining a room temperature of 24 °C. The blank group was fed with 23 g / rat of ordinary feed, while the model group, positive group (ALST), groups of Examples 1 - 4, and groups of Comparative Examples 1 - 3 were all fed with 23 g / rat of high-fat feed.

[0067] After 7 days of feeding the rats, they were randomly grouped:

[0068] Male: 8 rats in the blank group; 6 rats in the model group and Comparative Example 3 group; 5 rats in the positive group, groups of Examples 1 - 4, and Comparative Example 2 group; 4 rats in Comparative Example 1 group.

[0069] Except for the blank group and the model group, the positive group, groups of Examples 1 - 4, and groups of Comparative Examples 1 - 3 were intragastrically administered at a fixed dose of 3.78 g at the same time every day for 23 consecutive days. The food intake and body weight were recorded every day during the administration period.

[0070] II. Experimental results: The results are shown in Table 1 and Figure 1 .

[0071] The formula for calculating the body weight change rate is: (body weight after administration - body weight before administration) / body weight before administration × 100%

[0072] Table 1 Body weight change rate data

[0073]

[0074] Note: Compared with the model group, * indicates P < 0.05, and ** indicates P < 0.01.

[0075] From Table 1 and Figure 1 the following conclusions can be drawn:

[0076] By comparing the body weights of the rats in the blank group and the model group, there was a highly significant difference between the blank group and the model group, indicating that the model was successfully established ( Figure 1 A in

[0077] Compared with the model group, there were extremely significant differences in the body weight change rates on the 7th, 14th, and 23rd days after administration in the Example 1 group, indicating that the processed Atractylodes macrocephala Koidz. product of Example 1 had a significant curative effect, and the curative effect was proportional to time ( Figure 1 B-D in); compared with the model group, there was a significant difference in the body weight change rate on the 7th day after administration in the positive group and the Comparative Example 3 group, however, there were no significant differences in the body weight change rates on the 14th and 23rd days.

[0078] In summary, it shows that the Aurantii Fructus Immaturus-processed Atractylodes macrocephala Koidz. composition in the Example 1 group has a significant weight loss effect on diet-induced obesity, and the effect is better than that of the positive group, Examples 2-4, and Comparative Examples 1-3, providing ideas for the development of new obesity treatment products.

[0079] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than a limitation on the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art does not depart from the essence and scope of the technical solution of the present invention.

Claims

1. Use of a processed Atractylodes macrocephala product in preparing a drug for treating obesity, the preparation method of the processed Atractylodes macrocephala product comprising: weighing Immaturus citrus aurantium, adding 6-16 times the volume of water, soaking for 0-2.0h, boiling for 0.5-3h, filtering, repeatedly extracting the residue for 1-2 times, filtering, combining the filtrate, and concentrating to obtain Immaturus citrus aurantium juice; weighing raw Atractylodes macrocephala, adding Immaturus citrus aurantium juice, soaking for 12-36h, frying at 100-150°C for 4-12min, and then baking at 30-70°C for 24-72h; the weight of Immaturus citrus aurantium corresponding to the Immaturus citrus juice is 10% of that of the raw Atractylodes macrocephala; the obesity is diet-induced obesity.

2. The use according to claim 1, characterized in that The preparation method of the processed Atractylodes macrocephala is as follows: weigh the immature bitter orange, add 14.3 times the volume of water, soak for 0.5 hours, boil for 1.5 hours, filter, add 12 times the volume of water to the residue, boil for 1.5 hours, filter, combine the filtrate, concentrate, and obtain the immature bitter orange juice; weigh the raw Atractylodes macrocephala, add the immature bitter orange juice, soak for 24 hours, stir-fry at 110°C for 6 minutes, and then bake at 40°C for 24 hours.

3. The use according to claim 1, characterized in that The medicine further comprises a pharmaceutically acceptable carrier and / or excipient.

4. The use according to claim 1, characterized in that The dosage form of the medicine includes one or more of decoction, soup, tablet, granule and capsule.

5. The use according to claim 4, characterized in that The decoction is a water decoction.

6. The use according to claim 5, characterized in that The preparation method of the water decoction comprises the following steps: crushing the processed Atractylodes macrocephala, adding 6-10 times the volume of water, soaking for 20-40 minutes, refluxing extraction for 1-3 times, each time for 1-2 hours, filtering, collecting the filtrate, and concentrating at 60°C to obtain the decoction.

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