On-site Sampling and Detection Device and Method for Pig Manure for Detecting Drug Resistance in Piglet Diarrhea

Abstract

The present invention provides a device and method for on-site sampling and detection of pig manure for detecting drug resistance in piglet diarrhea, which relates to the technical field of sampling and detection. A clamping and rotating assembly is provided on a housing. A detection box is installed at the bottom of a sampling bottle. A piston housing and a liquid outlet control piston are installed on the outer wall of the sampling bottle. A pressure relief port is provided on the piston housing. A diaphragm is fixedly installed on the side wall of the sampling bottle. A liquid outlet cavity is arranged in the sampling bottle. A first through hole is provided on an inner housing. A second through hole is provided at the bottom of the sampling bottle. A first sealing ball and a second sealing ball are respectively installed in a constrained manner at the first through hole and the second through hole. A device pressing assembly for sample detection and a conveying assembly are also provided on the housing. The present invention realizes on-site sampling and detection of pig manure, ensures the timeliness of samples, and is detachably arranged, so that the piston housing, the liquid outlet control piston and the detection box can be reused, reducing the detection cost and avoiding cross-infection between samples, and improving the accuracy of detection results.

Description

Technical Field

[0001] The present invention relates to the technical field of sampling detection, and in particular to a device and method for on-site sampling and detection of pig manure for detecting the drug resistance of piglet diarrhea. Background Art

[0002] With the development of the breeding industry, diarrhea is one of the main causes of piglet death. In order to clarify the bacteria and drug resistance in piglet diarrhea, a common method is to collect pig manure into a sampling tube and send it to relevant departments for detection, analyze the bacteria contained in the pig manure, administer drugs to piglets according to the bacteria, and then sample again after drug administration to analyze the changes in bacteria in the pig manure, so as to obtain the drug resistance of the bacteria to such drugs. The pig manure samples collected by the traditional method need to be transported for a period of time before detection. During the transportation process, environmental factors change, and the original characteristics of the samples cannot be guaranteed, resulting in certain deviations in the detection results.

[0003] Therefore, there is a need for a device and method for on-site sampling and detection of pig manure for detecting the drug resistance of piglet diarrhea to solve the above problems. Summary of the Invention

[0004] The present invention provides a device and method for on-site sampling and detection of pig manure for detecting the drug resistance of piglet diarrhea, which realizes on-site sampling and detection of pig manure, ensures the timeliness of the samples, and also uses detachable disposable sampling bottles, glass slides and cover glasses, so that the piston housing, the liquid outlet control piston and the detection box can be reused, reducing the detection cost while avoiding cross-infection between samples and improving the accuracy of the detection results.

[0005] The technical solution of the present invention is realized as follows:

[0006] An on-site sampling and detection device for pig manure for detecting the drug resistance of piglet diarrhea, comprising a housing, and a mixing device for mixing the samples and reagents in the sampling bottle is arranged on the housing. The mixing device includes a rotating disk rotatably installed on the housing in the vertical direction, and at least one clamping and rotating assembly for clamping and driving the sampling bottle to rotate self is arranged on the top of the rotating disk;

[0007] The bottom of the sampling bottle is detachably installed with a detection box with an open top. A glass slide and a coverslip are detachably installed in the detection box. A piston housing and a liquid outlet control piston are detachably installed on the outer wall of the sampling bottle. The lower end of the liquid outlet control piston extends into the piston housing. A pressure relief port is provided on the side wall of the piston housing. A diaphragm is fixedly installed on the side wall of the sampling bottle opposite to the pressure relief port. An inner housing is arranged in the sampling bottle. A liquid outlet cavity is formed between the inner housing, the diaphragm and the sampling bottle. A first through hole is provided on the inner housing. A second through hole is provided at the bottom of the sampling bottle. A first sealing ball located in the sampling bottle is constrained and installed at the first through hole. A second sealing ball located in the liquid outlet cavity is constrained and installed at the second through hole;

[0008] The housing is also provided with a sample detection device and a conveying assembly for unlocking and conveying the detection box. A pressing assembly for pressing the coverslip is arranged between the sample detection device and the mixing device.

[0009] As a preferred technical solution, the rotating disk is horizontally arranged. The clamping and rotating assembly includes a rotating outer sleeve rotatably installed on the top of the rotating disk. A rotating inner sleeve is rotatably installed inside the rotating outer sleeve. Three sliding clamping blocks are slidably installed on the rotating outer sleeve along the radial direction. Each sliding clamping block is arranged above the rotating inner sleeve. Three arc-shaped grooves are provided on the top of the rotating inner sleeve. A fixing column is fixedly installed at the bottom of each sliding clamping block. Each fixing column is constrained and installed in the corresponding arc-shaped groove. The rotating inner sleeve is in transmission connection with an inner sleeve driving assembly. At least one avoidance hole is provided on the rotating disk. The avoidance hole is coaxially arranged with the rotating inner sleeve and the rotating outer sleeve.

[0010] As a preferred technical solution, a pair of first locking rods are fixedly installed at the bottom of the sampling bottle. A first end face is vertically arranged on each first locking rod. A pair of second locking rods are constrained and installed on the outer wall of the detection box. Two installation through grooves are provided on the detection box. The free end of each second locking rod passes through the corresponding installation through groove and is arranged inside the detection box. A second end face adapted to the corresponding first end face is vertically arranged at the free end of each second locking rod. A pair of handles are hinged on the outer wall of the detection box. A horizontally arranged push rod is fixedly installed at the free end of each handle. An arc-shaped rotating plate is hinged between each handle and the corresponding second locking rod. A guide block is provided on the top of each second locking rod. Each guide block is arranged outside the detection box.

[0011] As a preferred technical solution, a pair of horizontally arranged sliding column guiding holes are provided on the outer wall of the piston housing. A sliding column is slidably installed in each sliding column guiding hole. A first compression spring is arranged between each sliding column and the corresponding sliding column guiding hole. A connecting column is fixedly installed at the free end of each sliding column, and a limiting block is fixedly installed at the free end of each connecting column.

[0012] Two installation grooves are vertically arranged on the outer side wall of the sampling bottle. A pair of limiting block installation holes and limiting holes are horizontally arranged on the outer side wall of the sampling bottle. The upper end of each installation groove communicates with one of the limiting block installation holes, the lower end of each installation groove communicates with one of the limiting holes, each limiting block installation hole communicates with the adjacent limiting hole, the diameter of each limiting block installation hole is larger than the diameter of the corresponding limiting block, and the diameter of each limiting hole is smaller than the diameter of the corresponding limiting block.

[0013] As a preferred technical solution, the glass slide is horizontally installed in the test box in a detachable manner. The cover glass is arranged above the glass slide. A mounting plate is fixedly installed at both ends of the cover glass. A pair of damping sliding sleeves are fixedly installed on each mounting plate. Two pairs of vertically arranged damping guiding rods are fixedly installed in the test box. Each damping sliding sleeve is sleeved on the corresponding damping guiding rod. The damping sliding sleeve and the damping guiding rod do not move relative to each other without external force. A sample liquid flow groove is arranged on one of the mounting plates, and the sample liquid flow groove is arranged below the second through hole.

[0014] As a preferred technical solution, the conveying assembly includes a first sliding plate slidably installed horizontally below the sampling bottle. A second sliding plate is slidably installed vertically on the first sliding plate. Two push plates for pushing the corresponding handle to rotate are fixedly installed on the second sliding plate. A guiding inclined surface adapted to the corresponding push rod is arranged at the top of each push plate.

[0015] As a preferred technical solution, the pressing-down assembly includes a sliding rod slidably installed vertically on the housing. A pressure-relieving hole is arranged at the lower end of the sliding rod. A pressure-relieving column is slidably installed in the pressure-relieving hole. A horizontally arranged lower pressing plate is fixedly installed at the lower end of the pressure-relieving column. A second compression spring is arranged between the pressure-relieving column and the pressure-relieving hole. It also includes a right-angle connecting plate. The middle of the right-angle connecting plate is hinged to the upper end of the sliding rod through a connecting rod. One end of the right-angle connecting plate is hinged to the inner wall of the housing, and the other end of the right-angle connecting plate is hinged to a connecting plate driving cylinder, and the connecting plate driving cylinder is hinged to the inner wall of the housing.

[0016] As a preferred technical solution, the housing is provided with a detection chamber, the detection chamber is communicated with a vacuum generator, the detection device is arranged in the detection chamber, the sample detection device includes an electron source, two condenser lenses, a scanning coil and an objective lens fixedly installed on the housing from top to bottom, and a photoelectric detection element, a controller, a display screen and control buttons are also fixedly installed in the housing. The photoelectric detection element, the scanning coil, the display screen and the control buttons are all electrically connected to the controller.

[0017] As a preferred technical solution, a sample discharging assembly for discharging the detected test box is arranged on the housing. The sample discharging assembly includes a conveying groove. A sample discharging port is arranged on the housing on one side of the first sliding plate. The conveying groove is arranged between the horizontal conveying plate and the sample discharging port. A pushing box air cylinder for pushing the test box from the first sliding plate into the conveying groove is arranged on the other side of the first sliding plate.

[0018] The method of the on-site sampling and detection device for pig manure for detecting the drug resistance of piglet diarrhea specifically includes the following steps:

[0019] S1. Take an appropriate amount of piglet manure into the sampling bottle. Under the action of the mixing device, the sample in the sampling bottle is mixed evenly with the reagent to form a sample solution. At this time, the sampling bottle is located below the conveying assembly.

[0020] S2. Press down the liquid outlet control piston. The pressure inside the piston housing increases. The diaphragm bends and deforms towards the inside of the liquid outlet chamber. The pressure in the liquid outlet chamber is greater than the pressure in the sampling bottle. The first sealing ball moves towards the sampling bottle. The sample solution flows from the sampling bottle to the liquid outlet chamber through the first through hole.

[0021] S3. Pull up the liquid outlet control piston. The pressure inside the piston housing decreases. The diaphragm bends and deforms towards the inside of the piston housing. The pressure in the liquid outlet chamber is less than the pressure in the sampling bottle. The first sealing ball resets. The second sealing ball moves towards the liquid outlet chamber. The sample solution in the liquid outlet chamber flows through the second through hole to between the glass slide and the coverslip in the test box.

[0022] S4. Unlock the test box and the sampling bottle through the conveying assembly, and convey the test box to below the pressing assembly. The pressing assembly presses down the coverslip, and then the conveying assembly conveys the test box to below the detection device.

[0023] S5. The detection device detects the sample solution between the glass slide and the coverslip, and thus completes one-time on-site sampling and detection of piglet feces.

[0024] S6. After the detection is completed, remove the slide and the cover glass in the detection box, and remove the piston housing and the liquid outlet control piston on the sampling tube. The detection box, the piston housing and the liquid outlet control piston can be reused.

[0025] Adopting the above technical solution, the beneficial effects of the present invention are as follows:

[0026] Since the on-site sampling detection device for pig manure used for detecting the drug resistance of piglet diarrhea includes a detection box, during the use of this device, piglet feces samples are placed in a sampling bottle, the sampling bottle is placed on the housing and clamped by a clamping rotation assembly, the sampling bottle rotates under the drive of a rotating disk and the clamping rotation assembly, and the feces sample in the sampling bottle is mixed with a reagent to form a sample solution. Press down the liquid outlet control piston, the pressure in the piston housing increases, causing the diaphragm to bend and deform towards the liquid outlet cavity, the pressure in the liquid outlet cavity becomes larger, causing the first sealing ball to move towards the sampling bottle. The sample solution in the sampling bottle first flows into the liquid outlet cavity through the first through hole. Pull up the liquid outlet control piston, the pressure in the piston housing decreases, the diaphragm bends and deforms towards the piston housing, the pressure in the liquid outlet cavity is less than the pressure in the sampling bottle, the first sealing ball resets, making the pressure in the liquid outlet cavity less than the pressure in the detection box, the second sealing ball moves into the liquid outlet cavity, and the sample solution flows into the detection box through the second through hole. In the present invention, by pressing down and pulling up the liquid outlet control piston, the diaphragm deforms, realizing the adjustment of the pressure in the piston housing and the liquid outlet cavity, thus realizing the movement of the first sealing ball and the second sealing ball, and then realizing the alternating operation of the first through hole and the second through hole, and further realizing the flow of the sample solution from the collection bottle to the detection box.

[0027] After the sample solution flows into the detection box, the sample solution flows to the space between the slide and the cover glass through the sample solution flow groove. Then, the detection box is unlocked from the sampling bottle by the conveying assembly and conveyed to the lower part of the pressing assembly. The pressing assembly presses down the cover glass, and the conveying assembly conveys the detection box to the lower part of the detection device. The detection device detects the sample solution between the slide and the cover glass, thus completing an on-site sampling detection of piglet feces. In the present invention, through the cooperation of the mixing device, the conveying assembly, the pressing assembly and the detection device, the on-site sampling and detection of pig manure are realized, ensuring the timeliness of the sample. This device adopts detachable disposable sampling bottles, slides and cover glasses, making the piston housing, the liquid outlet control piston and the detection box reusable, reducing the detection cost while avoiding cross-infection between samples and improving the accuracy of the detection results.

[0028] Since the on-site sampling detection device for pig manure used for detecting the drug resistance of piglet diarrhea includes a mixing device, when using this device, the sampling bottle containing the sample is placed in one of the rotating inner sleeves. Driven by the inner sleeve drive assembly, the rotating inner sleeve rotates. As the rotating inner sleeve rotates, the arc-shaped groove pushes the fixed block to move, and the three sliding clamping blocks move towards the sampling bottle to clamp the sampling bottle. Then, through the rotation of the rotating outer sleeve, the self-rotation of the sampling bottle is realized. In the present invention, the rotation of the rotating disc and the self-rotation of the sampling bottle fully mix the sample and the reagent in the sampling bottle, improving the mixing efficiency.

[0029] Since the on-site sampling detection device for pig manure used for detecting the drug resistance of piglet diarrhea includes a conveying assembly, when the sample liquid flows out from the second through hole and falls into the sample liquid flow groove, at this time, the conveying device is located below the detection box, the second sliding plate moves upward, the guiding inclined surface abuts against the push rod and pushes the push rod to move away from the detection box. As the push rod moves, the handle rotates. Through the connection of the arc-shaped rotating plate, the hinged end of the second locking rod moves towards the first locking rod, the guiding block abuts against the detection box, and the free end of the second locking rod moves away from the first locking rod, and the first locking rod and the second locking rod are unlocked, that is, the unlocking of the detection box and the sampling bottle is realized.

[0030] Since the on-site sampling detection device for pig manure used for detecting the drug resistance of piglet diarrhea includes a pressing-down assembly, when the detection box and the sampling bottle are unlocked, the detection box falls onto the second sliding plate. As the first sliding plate moves, the glass slide moves to below the pressing plate. Driven by the connecting plate driving cylinder, the right-angled connecting plate rotates towards the connecting rod, and the connecting rod moves towards the sliding rod, causing the sliding rod to move downward in the vertical direction. During this process, the pressing plate first abuts against the top of the glass slide. As the sliding rod continues to press down, the second compression spring is compressed, and the glass slide receives a continuously slowly pressing-down force, which avoids the glass slide and the cover glass from being broken due to the strong pressing of the cover glass. Also, the sample liquid between the glass slide and the cover glass is evenly laid between the glass slide and the cover glass after being slowly stressed, facilitating subsequent sample detection.

[0031] Since the on-site sampling detection device for pig manure used for detecting the drug resistance of piglet diarrhea includes a detection device, when the conveying assembly conveys the detection box to below the detection device, the detection device starts to work. The electron beam emitted by the electron source passes through two condenser lenses and scans the sample surface in sequence, exciting the sample to generate various physical signals. The intensity of these physical signals varies with the surface characteristics of the sample. The physical signals are received by the photoelectric detection element and transmitted to the controller. The controller transmits it to the display screen, and the display screen displays the picture corresponding to the surface characteristics of the sample to analyze the sample. Description of the Drawings

[0032] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0033] Figure 1 is the structural schematic diagram of the present invention;

[0034] Figure 2 is Figure 1 the enlarged structural schematic diagram at position A in

[0035] Figure 3 is Figure 1 the enlarged structural schematic diagram at position B in

[0036] Figure 4 is the state reference diagram of the upper pull-out liquid control piston;

[0037] Figure 5 is Figure 4 the enlarged structural schematic diagram at position C in

[0038] Figure 6 is Figure 4 the enlarged structural schematic diagram at position D in

[0039] Figure 7 is the state reference diagram of sample detection;

[0040] Figure 8 is Figure 7 the sectional view in the E-E direction of

[0041] Figure 9 is the top view of the rotating disk;

[0042] Figure 10 is the structural schematic diagram of the sampling bottle;

[0043] Figure 11 is Figure 10 the enlarged structural schematic diagram at position F in

[0044] Figure 12 is the top view of the slide;

[0045] Figure 13 is the system structure block diagram of the PLC controller.

[0046] Wherein: 1. housing; 2. sampling bottle; 3. rotating disk; 4. detection box; 5. glass slide; 6. cover glass; 7. piston housing; 8. liquid outlet control piston; 9. pressure relief port; 10. diaphragm; 11. inner housing; 12. liquid outlet cavity; 13. first through hole; 14. second through hole; 15. first sealing ball; 16. second sealing ball; 17. rotating outer sleeve; 18. rotating inner sleeve; 19. sliding clamping block; 20. arc groove; 21. fixed column; 22. avoidance hole; 23. first locking rod; 24. first end face; 25. second locking rod; 26. installation through groove; 27. second end face; 28. handle; 29. push rod; 30. arc rotating plate; 31. guide block; 32. sliding column guide hole; 33. sliding column; 34. first compression spring; 35. connecting column; 36. limiting block; 37. installation groove; 38. limiting block installation hole; 39. limiting hole; 40. installation plate; 41. damping sliding sleeve; 42. damping guide rod; 43. sample liquid flow groove; 44. first sliding plate; 45. second sliding plate; 46. push plate; 47. guide inclined plane; 48. sliding rod; 49. pressure relief hole; 50. pressure relief column; 51. lower pressing plate; 52. second compression spring; 53. right-angle connecting plate; 54. connecting rod; 55. connecting plate driving cylinder; 56. detection cavity; 57. electron source; 58. condenser lens; 59. scanning coil; 60. objective lens; 61. photoelectric detection element; 62. controller; 63. display screen; 64. control button; 65. conveying groove; 66. sample discharging port; 67. pushing box cylinder; 68. hinge shaft; 69. through groove; 70. sealing door; 71. clamping block guide hole; 72. sealing cover; 73. sampling rod; 74. limiting plate; 75. limiting groove; 76. piston installation hole; 77. sliding groove; 78. convex block; 79. first driving motor; 80. driving gear; 81. first gear ring; 82. second gear ring; 83. driving bevel gear; 84. second driving motor; 85. slide rail; 86. first rodless cylinder; 87. vertical guide column; 88. second rodless cylinder. Detailed implementation mode

[0047] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0048] Embodiment 1:

[0049] As Figures 1 - 13As shown together, a pig manure on-site sampling and detection device for detecting the drug resistance of piglet diarrhea includes a housing 1. A mixing device for mixing the samples and reagents in the sampling bottle 2 is provided on the housing 1. The mixing device includes a rotating disk 3 rotatably installed on the housing 1 in the vertical direction. At least one clamping and rotating assembly for clamping and driving the sampling bottle 2 to rotate itself is provided on the top of the rotating disk 3.

[0050] As Figure 3 shown, the bottom of the sampling bottle 2 is detachably installed with a detection box 4 with an open top. A glass slide 5 and a coverslip 6 are detachably installed in the detection box 4. A piston housing 7 and a liquid outlet control piston 8 are detachably installed on the outer wall of the sampling bottle 2. The lower end of the liquid outlet control piston 8 extends into the piston housing 7. A pressure relief port 9 is provided on the side wall of the piston housing 7. A diaphragm 10 is fixedly installed on the side wall of the sampling bottle 2 opposite to the pressure relief port 9. An inner housing 11 is provided in the sampling bottle 2. An liquid outlet cavity 12 is formed between the inner housing 11, the diaphragm 10 and the sampling bottle 2. A first through hole 13 is provided on the inner housing 11. A second through hole 14 is provided at the bottom of the sampling bottle 2. A first sealing ball 15 located in the sampling bottle 2 is constrainedly installed at the first through hole 13. A second sealing ball 16 located in the liquid outlet cavity 12 is constrainedly installed at the second through hole 14.

[0051] The housing 1 is also provided with a sample detection device and a conveying assembly for unlocking and conveying the detection box 4. A pressing assembly for pressing down the coverslip 6 is provided between the sample detection device and the mixing device.

[0052] Among them, the rotating disk 3 is horizontally arranged. The clamping and rotating assembly includes a rotating outer sleeve 17 rotatably installed on the top of the rotating disk 3. A rotating inner sleeve 18 is rotatably installed inside the rotating outer sleeve 17. Three sliding clamping blocks 19 are slidably installed on the rotating outer sleeve 17 along the radial direction. Each sliding clamping block 19 is arranged above the rotating inner sleeve 18. Three arc-shaped grooves 20 are provided on the top of the rotating inner sleeve 18. A fixing column 21 is fixedly installed at the bottom of each sliding clamping block 19. Each fixing column 21 is constrainedly installed in the corresponding arc-shaped groove 20. The rotating inner sleeve 18 is in wheel transmission connection with an inner sleeve driving assembly. At least one avoidance hole 22 is provided on the rotating disk 3. The avoidance hole 22 is coaxially arranged with the rotating inner sleeve 18 and the rotating outer sleeve 17.

[0053] Moreover, a pair of first locking rods 23 are fixedly installed at the bottom of the sampling bottle 2. Each first locking rod 23 is vertically provided with a first end face 24. A pair of second locking rods 25 are constrained and installed on the outer wall of the detection box 4. There are two installation through slots 26 provided on the detection box 4. The free end of each second locking rod 25 passes through the corresponding installation through slot 26 and is arranged inside the detection box 4. The free end of each second locking rod 25 is vertically provided with a second end face 27 adapted to the corresponding first end face 24. A pair of handles 28 are hinged on the outer wall of the detection box 4. A horizontally arranged push rod 29 is fixedly installed at the free end of each handle 28. An arc-shaped rotating plate 30 is hinged between each handle 28 and the corresponding second locking rod 25. A guide block 31 is provided on the top of each second locking rod 25. Each guide block 31 is arranged outside the detection box 4. In the present invention, when the detection box 4 and the sampling bottle 2 are in a locked state, the first end face 24 and the corresponding second end face 27 are in a butting state.

[0054] In addition, a pair of horizontally arranged sliding column guide holes 32 are provided on the outer wall of the piston housing 7. A sliding column 33 is slidably installed in each sliding column guide hole 32. A first compression spring 34 is provided between each sliding column 33 and the corresponding sliding column guide hole 32. A connecting column 35 is fixedly installed at the free end of each sliding column 33. A limiting block 36 is fixedly installed at the free end of each connecting column 35.

[0055] As Figure 2 、 Figure 9 and Figure 10 collectively shown, two installation grooves 37 are vertically provided on the outer side wall of the sampling bottle 2. A pair of limiting block installation holes 38 and limiting holes 39 are horizontally provided on the outer side wall of the sampling bottle 2. The upper end of each installation groove 37 communicates with one of the limiting block installation holes 38, and the lower end of each installation groove 37 is connected to one of the limiting holes 39. Each limiting block installation hole 38 is connected to the adjacent limiting hole 39. The diameter of each limiting block installation hole 38 is larger than the diameter of the corresponding limiting block 36, and the diameter of each limiting hole 39 is smaller than the diameter of the corresponding limiting block 36.

[0056] Secondly, the glass slide 5 is horizontally installed in the detection box 4 in a detachable manner. The cover glass 6 is arranged above the glass slide 5. A mounting plate 40 is fixedly installed at both ends of the cover glass 6. A pair of damping sliding sleeves 41 are fixedly installed on each mounting plate 40. Two pairs of vertically arranged damping guide rods 42 are fixedly installed in the detection box 4. Each damping sliding sleeve 41 is sleeved on the corresponding damping guide rod 42. The damping sliding sleeve 41 and the damping guide rod 42 do not move relative to each other without external force. A sample liquid flow groove 43 is provided on one of the mounting plates 40. The sample liquid flow groove 43 is arranged below the second through hole 14.

[0057] The conveying assembly includes a first sliding plate 44 slidably mounted horizontally below the sampling bottle 2. A second sliding plate 45 is slidably mounted vertically on the first sliding plate 44. Two push plates 46 for pushing the corresponding handle 28 to rotate are fixedly mounted on the second sliding plate 45. A guiding inclined surface 47 adapted to the corresponding push rod 29 is provided at the top of each push plate 46.

[0058] The pressing-down assembly includes a sliding rod 48 slidably mounted vertically on the housing 1. A pressure-relieving hole 49 is provided at the lower end of the sliding rod 48. A pressure-relieving column 50 is slidably mounted in the pressure-relieving hole 49. A horizontally arranged lower pressing plate 51 is fixedly mounted at the lower end of the pressure-relieving column 50. A second compression spring 52 is provided between the pressure-relieving column 50 and the pressure-relieving hole 49. It further includes a right-angle connecting plate 53. The middle of the right-angle connecting plate 53 is hinged to the upper end of the sliding rod 48 through a connecting rod 54. One end of the right-angle connecting plate 53 is hinged to the inner wall of the housing 1. The other end of the right-angle connecting plate 53 is hinged to a connecting plate driving cylinder 55, and the connecting plate driving cylinder 55 is hinged to the inner wall of the housing 1.

[0059] As Figure 1 and Figure 13 As shown in [relevant figures] together, the housing 1 is provided with a detection chamber 56. The detection chamber 56 is communicated with a vacuum generator. The detection device is arranged in the detection chamber 56. The sample detection device includes an electron source 57, two condenser lenses 58, a scanning coil 59 and an objective lens 60 fixedly mounted on the housing 1 from top to bottom. An optoelectronic detection element 61, a controller 62, a display screen 63 and control buttons 64 are also fixedly mounted in the housing 1. The optoelectronic detection element 61, the scanning coil 59, the display screen 63 and the control buttons 64 are all electrically connected to the controller 62. In the present invention, the vacuum generator uses an air pump, the controller uses a single-chip microcomputer of the HD64F36109HV model of Renesas Corporation, the electron source 57 can use a laser or a laser diode, and the optoelectronic detection element 61 is an optoelectronic detector composed of a plurality of photodiodes.

[0060] As Figure 8 As shown in [relevant figure], a sample discharging assembly for discharging the detected detection box 4 is provided on the housing 1. The sample discharging assembly includes a conveying groove 65. A sample discharging port 66 is provided on the housing 1 on one side of the first sliding plate 44. The conveying groove 65 is arranged between the horizontal conveying plate and the sample discharging port 66. A box pushing cylinder 67 for pushing the detection box 4 from the first sliding plate 44 into the conveying groove 65 is provided on the other side of the first sliding plate 44.

[0061] The bottom inner wall of the liquid outlet chamber 12 is inclined towards the direction of the second through hole 14.

[0062] Each second locking rod 25 is installed on the corresponding detection box 4 through a hinge shaft 68. Each second locking rod 25 is provided with a constraint through groove 69, and each hinge shaft 68 is constrained and installed in the corresponding sliding rod through groove 69.

[0063] A sealing door 70 is provided at the nesting opening 66.

[0064] Three clamping block guiding holes 71 are provided on the side wall of each rotating outer sleeve 17. The outer end of each sliding clamping block 19 is slidably installed in the corresponding clamping block guiding hole 71, and the inner end face of each sliding clamping block 19 is arc-shaped.

[0065] The upper openings of the sampling bottles 2 are all threaded with sealing caps 72. A sampling rod 73 is fixedly installed at the bottom of the sealing cap 72. Three limiting plates 74 are fixedly installed on the outer side walls of the sampling bottles 2. Three limiting grooves 75 are provided on the rotating inner sleeve 18. Each limiting groove 75 is adapted to the corresponding limiting plate 74. An outlet control piston mounting hole 76 is provided on one of the limiting plates 74.

[0066] A plurality of sliding grooves 77 are provided on the top of the rotating disk 3. A convex block 78 is fixedly installed at the bottom of each rotating outer sleeve 17. Each convex block 78 is constrained and installed in the corresponding sliding groove 77. A first driving motor 79 is fixedly installed on the housing 1. The first driving motor 79 is in transmission connection with the rotating disk 3. A driving gear 80 is fixedly installed on the motor shaft of the first driving motor 79. A first toothed ring 81 is fixedly installed on the outer circumferential surface of the rotating outer sleeve 17. The first toothed ring 81 is in transmission connection with the driving gear 80.

[0067] The inner sleeve driving assembly includes a second toothed ring 82 fixedly installed at the bottom of the rotating inner sleeve 18. Three driving bevel gears 83 are rotatably installed on each rotating outer sleeve 17. One of the driving bevel gears 83 is in transmission connection with a second driving motor 84. Each driving bevel gear 83 meshes with the corresponding second toothed ring 82.

[0068] A slide rail 85 is horizontally arranged in the housing 1. The first sliding plate 44 is slidably installed on the slide rail 85. A first rodless cylinder 86 is fixed in the housing 1. The main body of the first rodless cylinder 86 is connected to the first sliding plate 44. Four vertical guide posts 87 and a pair of second rodless cylinders 88 are symmetrically arranged on the first sliding plate 44. The second rodless cylinders 88 are vertically arranged. The second sliding plate 45 is slidably installed on the four vertical guide posts 87. The bodies of the two second rodless cylinders 88 are fixedly connected to the second sliding plate 45.

[0069] Embodiment 2:

[0070] The method of the on-site pig manure sampling and detection device for detecting the drug resistance of piglet diarrhea specifically includes the following steps:

[0071] S1. Take an appropriate amount of piglet feces and place them in the sampling bottle 2. Under the action of the mixing device, the sample in the sampling bottle 2 is evenly mixed with the reagent to form a sample solution. At this time, the sampling bottle 2 is located below the conveying component.

[0072] S2. Press down the liquid outlet control piston 8. The pressure inside the piston housing 7 increases, the diaphragm 10 bends and deforms towards the inside of the liquid outlet cavity 12. The pressure in the liquid outlet cavity 12 is greater than the pressure in the sampling bottle 2, and the first sealing ball 15 moves towards the sampling bottle 2. The sample solution flows from the sampling bottle 2 into the liquid outlet cavity 12 through the first through hole 13.

[0073] S3. Pull up the liquid outlet control piston 8. The pressure inside the piston housing 7 decreases, the diaphragm 10 bends and deforms towards the inside of the piston housing 7. The pressure in the liquid outlet cavity 12 is less than the pressure in the sampling bottle 2, the first sealing ball 15 resets, and the second sealing ball 16 moves into the liquid outlet cavity 12. The sample solution in the liquid outlet cavity 12 flows between the glass slide 5 and the cover glass 6 in the detection box 4 through the second through hole 14.

[0074] S4. Unlock the detection box 4 and the sampling bottle 2 through the conveying component, and convey the detection box 4 below the pressing component. The pressing component presses down the cover glass 6, and then the conveying component conveys the detection box 4 below the detection device.

[0075] S5. The detection device detects the sample solution between the glass slide 5 and the cover glass 6, and thus completes one-time on-site sampling and detection of piglet feces.

[0076] S6. After the detection is completed, remove the glass slide 5 and the cover glass 6 in the detection box 4, and remove the piston housing 7 and the liquid outlet control piston 8 on the sampling tube. The detection box 4, the piston housing 7 and the liquid outlet control piston 8 can be reused.

[0077] In summary, the present invention realizes on-site sampling and detection of pig manure, ensures the timeliness of the sample, and also adopts detachable disposable sampling bottles, glass slides and cover glasses, enabling the piston housing, the liquid outlet control piston and the detection box to be reused. While reducing the detection cost, it also avoids cross-infection between samples and improves the accuracy of the detection results.

[0078] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A pig manure on-site sampling and detection device for detecting drug resistance in piglet diarrhea, comprising a housing, characterized in that, A mixing device for mixing the sample and reagent in the sampling bottle is provided on the housing. The mixing device includes a rotating disk rotatably mounted on the housing in the vertical direction, and at least one clamping and rotating assembly for clamping and driving the sampling bottle to rotate itself is provided on the top of the rotating disk; The bottom of the sampling bottle is detachably installed with a detection box with an open top. A glass slide and a coverslip are detachably installed in the detection box. A piston housing and a liquid outlet control piston are detachably installed on the outer wall of the sampling bottle. The lower end of the liquid outlet control piston extends into the piston housing. A pressure relief port is provided on the side wall of the piston housing. A diaphragm is fixedly installed on the side wall of the sampling bottle opposite to the pressure relief port. An inner housing is provided in the sampling bottle. A liquid outlet cavity is formed between the inner housing, the diaphragm and the sampling bottle. A first through hole is provided on the inner housing. A second through hole is provided at the bottom of the sampling bottle. A first sealing ball located in the sampling bottle is constrainedly installed at the first through hole. A second sealing ball located in the liquid outlet cavity is constrainedly installed at the second through hole; A sample detection device and a conveying assembly for unlocking and conveying the detection box are further provided on the housing. A pressing assembly for pressing the coverslip is provided between the sample detection device and the mixing device; The rotating disk is horizontally arranged. The clamping and rotating assembly includes a rotating outer sleeve rotatably installed on the top of the rotating disk. A rotating inner sleeve is rotatably installed inside the rotating outer sleeve. Three sliding clamping blocks are slidably installed on the rotating outer sleeve along the radial direction. Each sliding clamping block is arranged above the rotating inner sleeve. Three arc-shaped grooves are provided on the top of the rotating inner sleeve. A fixing column is fixedly installed at the bottom of each sliding clamping block. Each fixing column is constrainedly installed in the corresponding arc-shaped groove. The rotating inner sleeve is in wheel transmission connection with an inner sleeve driving assembly. At least one avoidance hole is provided on the rotating disk. The avoidance hole is coaxially arranged with the rotating inner sleeve and the rotating outer sleeve.

2. The on-site sampling and detection device for pig manure used for detecting drug resistance of piglet diarrhea according to claim 1, wherein, A pair of first locking rods are fixedly installed at the bottom of the sampling bottle. A first end face is vertically provided on each first locking rod. A pair of second locking rods are constrainedly installed on the outer wall of the detection box. Two installation through grooves are provided on the detection box. The free end of each second locking rod passes through the corresponding installation through groove and is arranged inside the detection box. A second end face adapted to the corresponding first end face is vertically provided at the free end of each second locking rod. A pair of handles are hinged on the outer wall of the detection box. A horizontally arranged push rod is fixedly installed at the free end of each handle. An arc-shaped rotating plate is hinged between each handle and the corresponding second locking rod. A guide block is provided on the top of each second locking rod. Each guide block is arranged outside the detection box.

3. The on-site sampling and detection device for pig manure used for detecting drug resistance of piglet diarrhea according to claim 1, wherein, A pair of horizontally arranged sliding column guide holes are provided on the outer wall of the piston housing. A sliding column is slidably installed in each sliding column guide hole. A first compression spring is arranged between each sliding column and the corresponding sliding column guide hole. A connecting column is fixedly installed at the free end of each sliding column, and a limiting block is fixedly installed at the free end of each connecting column. Two installation grooves are vertically arranged on the outer side wall of the sampling bottle. A pair of limiting block installation holes and limiting holes are horizontally arranged on the outer side wall of the sampling bottle. The upper end of each installation groove communicates with one of the limiting block installation holes, and the lower end of each installation groove is connected to one of the limiting holes. Each limiting block installation hole is connected to the adjacent limiting hole. The diameter of each limiting block installation hole is larger than the diameter of the corresponding limiting block, and the diameter of each limiting hole is smaller than the diameter of the corresponding limiting block.

4. The on-site sampling and detection device for pig manure used for detecting drug resistance of piglet diarrhea according to claim 1, characterized in that, The glass slide is horizontally installed in the detection box in a detachable manner. The cover glass is arranged above the glass slide. A mounting plate is fixedly installed at both ends of the cover glass. A pair of damping sliding sleeves are fixedly installed on each mounting plate. Two pairs of vertically arranged damping guide rods are fixedly installed in the detection box. Each damping sliding sleeve is sleeved on the corresponding damping guide rod. The damping sliding sleeve and the damping guide rod do not move relative to each other without external force. A sample liquid flow groove is arranged on one of the mounting plates, and the sample liquid flow groove is arranged below the second through hole.

5. The on-site sampling and detection device for pig manure used for detecting drug resistance of piglet diarrhea according to claim 2, wherein The conveying assembly includes a first sliding plate slidably installed horizontally below the sampling bottle. A second sliding plate is slidably installed vertically on the first sliding plate. Two push plates for pushing the corresponding handle to rotate are fixedly installed on the second sliding plate. A guiding inclined surface adapted to the corresponding push rod is arranged at the top of each push plate.

6. The on-site sampling and detection device for pig manure used for detecting the drug resistance of piglet diarrhea according to claim 2, wherein, The pressing-down assembly includes a sliding rod slidably installed vertically on the housing. A pressure-relieving hole is arranged at the lower end of the sliding rod. A pressure-relieving column is slidably installed in the pressure-relieving hole. A horizontally arranged lower pressing plate is fixedly installed at the lower end of the pressure-relieving column. A second compression spring is arranged between the pressure-relieving column and the pressure-relieving hole. It also includes a right-angle connecting plate. The middle of the right-angle connecting plate is hinged to the upper end of the sliding rod through a connecting rod. One end of the right-angle connecting plate is hinged to the inner wall of the housing, and the other end of the right-angle connecting plate is hinged to a connecting plate driving cylinder, and the connecting plate driving cylinder is hinged to the inner wall of the housing.

7. The on-site pig manure sampling and detection device for detecting drug resistance of piglet diarrhea according to claim 1, wherein, The housing is provided with a detection cavity. The detection cavity is communicated with a vacuum generator. The detection device is arranged in the detection cavity. The sample detection device includes an electron source, two condenser lenses, a scanning coil, and an objective lens fixedly installed on the housing from top to bottom. A photoelectric detection element, a controller, a display screen, and control buttons are also fixedly installed in the housing. The photoelectric detection element, the scanning coil, the display screen, and the control buttons are all electrically connected to the controller.

8. The on-site sampling and detection device for pig manure used for detecting the drug resistance of piglet diarrhea according to claim 5, characterized in that, A sampling component for discharging the detected test box is arranged on the shell. The sampling component includes a conveying groove. A sampling port is arranged on the shell on one side of the first sliding plate. The conveying groove is arranged between the horizontal conveying plate and the sampling port. A push box cylinder for pushing the test box from the first sliding plate into the conveying groove is arranged on the other side of the first sliding plate.

9. A method for using the on-site sampling and detection device for pig manure for detecting drug resistance of piglet diarrhea according to any one of claims 1-8, characterized in that, Specifically, it includes the following steps: S1. Take an appropriate amount of piglet feces and place it in the sampling bottle. Under the action of the mixing device, the sample in the sampling bottle is evenly mixed with the reagent to form a sample solution. At this time, the sampling bottle is located below the conveying component. S2. Press down the liquid outlet control piston. The pressure inside the piston housing increases. The diaphragm bends and deforms towards the inside of the liquid outlet cavity. The pressure in the liquid outlet cavity is greater than the pressure in the sampling bottle. The first sealing ball moves towards the sampling bottle. The sample solution flows from the sampling bottle to the liquid outlet cavity through the first through hole. S3. Pull up the liquid outlet control piston. The pressure inside the piston housing decreases. The diaphragm bends and deforms towards the inside of the piston housing. The pressure in the liquid outlet cavity is less than the pressure in the sampling bottle. The first sealing ball resets. The second sealing ball moves towards the liquid outlet cavity. The sample solution in the liquid outlet cavity flows through the second through hole to between the glass slide and the coverslip in the test box. S4. Unlock the test box and the sampling bottle through the conveying component, and convey the test box to below the pressing component. The pressing component presses down the coverslip, and then the conveying component conveys the test box to below the detection device. S5. The detection device detects the sample solution between the glass slide and the coverslip, and thus completes a on-site sampling and detection of piglet feces. S6. After the detection is completed, remove the glass slide and the coverslip in the test box, and remove the piston housing and the liquid outlet control piston on the sampling tube. The test box, the piston housing and the liquid outlet control piston can be reused.

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