A method for preventing and treating seabuckthorn cluster tip blight

Abstract

The application discloses a prevention and treatment method of Hippophae rhamnoides L. cluster top disease, which adopts a physical prevention and treatment method of spraying potassium permanganate in mid-April and spraying microbial agent + phosphorus and potassium compound foliar fertilizer from early May in combination with artificial removal to prevent and treat the Hippophae rhamnoides L. cluster top disease. The prevention and treatment method can effectively inhibit the development of the Hippophae rhamnoides L. cluster top disease. Field prevention and treatment tests show that after the prevention and treatment method is used, the plants with the Hippophae rhamnoides L. cluster top disease can grow a large number of symptomless tender branches in autumn, and the plants with the disease in the second year are all free of the cluster top symptom and in a healthy state. The prevention and treatment method has a good prevention and control effect on the Hippophae rhamnoides L. cluster top disease and is suitable for popularization and application in Hippophae rhamnoides L. cluster top disease prevention and control practices.

Description

Technical Field

[0001] This application relates to the field of plant disease control, and more specifically, to a method for controlling sea buckthorn tuft top disease. Background Technology

[0002] In recent years, a new disease has emerged on sea buckthorn, such as Figure 1 As shown, in the early stages of the disease, one or more branches simultaneously exhibit curling of the tender buds and leaves at the branch tips. Figure 1 In the case of disease B), as the disease spreads, the leaves at the tips of the young branches of the entire plant clump together and form spherical clusters, leading to the death of the affected branches the following year. Generally, the entire plant will die within 2-3 years of infection (e.g., ...). Figure 2 (As shown). Currently, this disease has occurred and caused damage on a large scale in Inner Mongolia Autonomous Region, Liaoning Province, and Hebei Province, and there is a trend towards a pandemic. More than 7,000 mu of sea buckthorn plantations belonging to Chengde Yuhangren Alpine Plant Application Technology Co., Ltd. in Weichang County have been affected to varying degrees. Furthermore, current research indicates that once this disease occurs, it is difficult to control. The inventors' team, together with experts from the Institute of Forest Ecology, Environment and Nature Conservation of the Chinese Academy of Forestry, conducted a preliminary study on the pathogen of this new disease appearing on sea buckthorn. The results showed that phytoplasma was not detected in any of the diseased samples (phytoplasma infection usually causes clustered leaf symptoms), ruling out the possibility of phytoplasma as the pathogen; in the transcriptome results, only a few samples tested positive for viruses, indicating that viruses are not the main pathogen; high-throughput sequencing and pathogen isolation methods also failed to obtain other potential pathogens; electron microscopy observation of the diseased tissue revealed that the pathogen is suspected to be a type of bacteria located in the xylem and phloem that cannot yet be artificially cultured. Figure 3 The specific type of pathogen still needs further clarification. Currently, based on the symptoms, the disease is named sea buckthorn cluster top disease.

[0003] In 2023 (Patent No.: ZL2023107260973), Xia Bo et al. proposed a method for controlling sea buckthorn leaf curl disease, disclosing that the pathogen is a virus. They described a method using a drug composed of spirotetramat, tannic acid, nicotinic acid, emulsifiable concentrate emamectin benzoate, and amino oligosaccharides, sprayed at specific stages of sea buckthorn growth and development, which showed some effectiveness in controlling the disease. However, due to the different pathogens, the virus-based control method in patent ZL2023107260973 is not applicable to the control of sea buckthorn cluster top disease in Weichang County. Given the increasingly serious damage caused by sea buckthorn cluster top disease, there is an urgent need for an effective control measure. Summary of the Invention

[0004] This invention provides a method for the prevention and control of sea buckthorn cluster top disease, comprising the following steps:

[0005] (1) In mid-April, during the leaf-bearing period of sea buckthorn, spray with potassium permanganate solution;

[0006] (2) In early May, microbial inoculants and foliar fertilizer are sprayed every 7 days, and 3 times of spraying is a spraying cycle; the microbial inoculants are selected from a combined inoculant of Bacillus subtilis and Paenibacillus polymyxa; the foliar fertilizer is a phosphorus-potassium compound foliar fertilizer containing trace elements;

[0007] (3) When it is found that the leaves of the sea buckthorn have been dead and become spherical during the spraying process, the diseased branches are manually cut off and collected for unified burning.

[0008] Specifically, the microbial inoculants are selected from 2.5×10 8 -2.5×10 9 viable bacteria / mL of Bacillus subtilis and 2.5×10 8 -2.5×10 9 viable bacteria / mL of Paenibacillus polymyxa; the foliar fertilizer comprises the following components in parts by weight: 20-30 parts of potassium nitrate, 70-90 parts of potassium dihydrogen phosphate, and 5 parts of trace elements, wherein the trace elements comprise the following components in parts by weight: 2 parts of zinc, 1 part of boron, 0.5 part of copper, 0.5 part of iron, 0.5 part of manganese, and 0.5 part of molybdenum; a mixed solution is obtained by mixing 200 ml of the Bacillus subtilis, 200 ml of the Paenibacillus polymyxa, and 100-150 g of the phosphorus-potassium compound foliar fertilizer according to a proportion, and the mixed solution is diluted and sprayed.

[0009] More specifically, the microbial inoculants are selected from 2.5×10 8 viable bacteria / mL of Bacillus subtilis and 2.5×10 8 viable bacteria / mL of Paenibacillus polymyxa; the foliar fertilizer comprises the following components in parts by weight: 20 parts of potassium nitrate, 75 parts of potassium dihydrogen phosphate, and 5 parts of trace elements; a mixed solution is obtained by mixing 200 ml of the Bacillus subtilis, 200 ml of the Paenibacillus polymyxa, and 100 g of the phosphorus-potassium compound foliar fertilizer according to a proportion.

[0010] The mass percentage concentration of the potassium permanganate solution is 2.5‰, and the dosage per mu is 50-60 liters.

[0011] The dosage of the mixed solution of the microbial inoculants and the foliar fertilizer per mu is 500-600 ml, and the mixed solution is diluted 100 times for application.

[0012] The potassium permanganate solution and the mixed solution of the microbial inoculants and the foliar fertilizer are uniformly sprayed on the tree trunks, branches, leaves, weeds, and land in the planting garden.

[0013] The step (2) further comprises spraying another cycle after 15-20 days from the end of the first spraying cycle.

[0014] The beneficial effects of the present application include:

[0015] According to the previous research results of the seabuckthorn cluster top disease pathogen, the physical prevention method of spraying potassium permanganate in mid-April, spraying microbial agent + phosphorus and potassium compound foliar fertilizer from early May, and artificial removal is selected to prevent and control the seabuckthorn cluster top disease, and the prevention and control method can effectively inhibit the development of the seabuckthorn cluster top disease. Field control test shows that after the prevention and control method is used, the diseased seabuckthorn plants can grow many and long symptomless shoots in autumn, and the diseased seabuckthorn plants have no cluster top symptoms in the second year, and show a healthy state. The prevention and control method has good prevention and control effect on the seabuckthorn cluster top disease, and is suitable for popularization and application in the seabuckthorn cluster top disease prevention and control practice. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 Fig. 1 is a symptom diagram of seabuckthorn cluster top disease, wherein A is a healthy plant diagram, and B is a symptom diagram of seabuckthorn cluster top disease;

[0017] Figure 2 Fig. 2 is a seabuckthorn tree killed by seabuckthorn cluster top disease;

[0018] Figure 3 Fig. 3 is an electron microscope diagram of a diseased seabuckthorn leaf;

[0019] Figure 4 Fig. 4 is a prevention and control effect diagram of treatment 2 in embodiment 1, wherein A is a new shoot diagram of seabuckthorn in autumn after treatment 2, and B is a healthy state diagram of branch in the next year after treatment 2. DETAILED DESCRIPTION

[0020] The application will be further described and explained with reference to the following examples. The described examples are only a part of the embodiments of the application, but not all the embodiments. Based on the application and the examples, all other applications and embodiments obtained by those skilled in the art without creative labor are within the protection scope of the application.

[0021] In the following examples, the experimental methods used are conventional methods unless otherwise specified.

[0022] In the following examples, the materials, reagents, etc. used are commercially available unless otherwise specified.

[0023] Example 1, prevention and control test of seabuckthorn cluster top disease

[0024] For seabuckthorn orchards with a high incidence of seabuckthorn cluster top disease, felling and destruction are not feasible because some seemingly healthy plants may already be infected by the pathogen, even if they haven't yet developed symptoms. In this situation, the inventors, based on previous research on the classification of the pathogen causing seabuckthorn cluster top disease, selected two groups of agents combined with physical control measures for a comparative efficacy test. Treatment 1 used a 2.5‰ potassium permanganate solution, and Treatment 2 used potassium permanganate plus microbial inoculants and a phosphorus-potassium compound foliar fertilizer. A control group was selected by spraying with plain water. The experimental site was a affected seabuckthorn plantation of Chengde Yuhangren Alpine Plant Application Technology Co., Ltd. Specific treatments are as follows:

[0025] Treatment 1: Spray with a 2.5‰ potassium permanganate solution

[0026] The specific steps for step 1 are as follows:

[0027] (1) In mid-April 2022, during the leafing period of sea buckthorn, a 2.5‰ (mass percentage) potassium permanganate solution was evenly sprayed onto the trunk, branches, leaves, weeds, and ground in the plantation. The amount of potassium permanganate solution used was 50 liters per mu.

[0028] (2) Starting in early May, spray a 2.5‰ (by weight) potassium permanganate solution every 7 days for 3 consecutive times, constituting one spraying cycle. When spraying, ensure even coverage of the tree trunk, branches, leaves, weeds, and ground in the plantation. The dosage of potassium permanganate solution is 50 liters per acre. After the first spraying cycle ends, spray for another cycle 20 days later.

[0029] (3) If the sea buckthorn leaves are found to be dead and spherical during the spraying process, the diseased branches should be manually cut off and collected for unified burning.

[0030] Treatment 2: Spraying microbial inoculants and foliar fertilizer

[0031] Microbial inoculant of 2.5×10⁻⁶ was selected. 8 live bacteria / mL of Bacillus subtilis

[0032] subtilis agent + 2.5×10 8 viable bacteria / mL of Paenibacillus

[0033] Polymyxa agent. The foliar fertilizer should be a phosphorus and potassium compound foliar fertilizer, comprising the following components by weight: 20 parts potassium nitrate, 75 parts potassium dihydrogen phosphate, and 5 parts trace elements, wherein the trace elements include the following components by weight ratio: 2 parts zinc, 1 part boron, 0.5 parts copper, 0.5 parts iron, 0.5 parts manganese, and 0.5 parts molybdenum. Specifically, mix 200 ml of Bacillus subtilis agent with...

[0034] 200ml Bacillus polymyxa agent and 100g of the potassium phosphate compound foliar fertilizer are mixed in a ratio to obtain a mixed solution, and the mixed solution is diluted 100 times and used for spraying.

[0035] The specific treatment of treatment 2 is as follows:

[0036] (1) In the middle of April 2022, during the leafing period of sea buckthorn, 2.5‰ (mass percentage) potassium permanganate solution was uniformly sprayed on the tree trunk, branches, leaves, and weeds and soil in the plantation, with a dosage of 50 liters per mu.

[0037] (2) Starting in early May, the diluted solution of the mixed solution of the microbial agent and the foliar fertilizer described above was sprayed once every 7 days, for 3 times as a spraying period. When spraying, the tree trunk, branches, leaves, and weeds and soil in the plantation were uniformly sprayed. The spraying amount was 50 liters per mu. After 20 days of the end of the first spraying period, another period was sprayed.

[0038] (3) During the spraying process, when the sea buckthorn leaves were found to be severely dead and spherical, etc., the diseased branches were manually cut off and collected for unified burning.

[0039] Field control effect investigation was carried out in autumn of the same year and spring of the next year. The results showed that in the test field sprayed with 2.5‰ potassium permanganate (treatment 1), the plants infected with sea buckthorn cluster tip disease had new symptom-free shoots in autumn, but the number was less and the shoots were shorter than those of treatment 2. The second year, the diseased sea buckthorn plants still had cluster tip symptoms, but the symptoms were lighter than the control group. However, after the combined use of microbial agents and foliar fertilizers, the diseased sea buckthorn plants had more and longer symptom-free shoots in autumn, and the second year, the diseased sea buckthorn plants had no cluster tip symptoms, showing a healthy state (as shown in Figure 4 The use of potassium permanganate and the combination of microbial agents composed of Bacillus subtilis and Bacillus polymyxa and potassium phosphate compound foliar fertilizer has good prevention and control effect on sea buckthorn cluster tip disease, and is suitable for popularization and application in practice.

Claims

1. A method for controlling Hippophae rhamnoides cluster tip blight, characterized by, The method comprises the following steps: (1) in the middle of April, when the sea-buckthorn leaves, spray potassium permanganate solution; (2) in early May, spray microbial agent and foliar fertilizer every 7 days, for 3 times as a spraying period; the microbial agent is bacillus subtilis agent and paenibacillus polymyxa agent; the foliar fertilizer is phosphorus and potassium compound foliar fertilizer containing trace elements; (3) when the sea-buckthorn leaves are found to be dead and spherical, cut off the diseased branches manually and collect them for burning; The microbial inoculant is selected from 2.5 x 10 8 -2.5 x 10 9 viable cells / mL of Bacillus subtilis inoculant and 2.5 x 10 8 -2.5 x 10 9 viable cells / mL of Paenibacillus polymyxa inoculant; The foliar fertilizer comprises the following components in parts by weight: potassium nitrate 20-30 parts, potassium dihydrogen phosphate 70-90 parts, and trace elements 5 parts, wherein the trace elements comprise the following components in parts by weight: zinc 2 parts, boron 1 part, copper 0.5 part, iron 0.5 part, manganese 0.5 part, and molybdenum 0.5 part; The mixed solution is obtained by mixing 200 ml of bacillus subtilis agent, 200 ml of paenibacillus polymyxa agent, and 100-150 g of the phosphorus and potassium compound foliar fertilizer, and the mixed solution is diluted and sprayed.

2. The control method according to claim 1, characterized by, The microbial agent is selected from 2.5 x 10 8 live bacteria / mL of Bacillus subtilis agent and 2.5 x 10 8 live bacteria / mL of Paenibacillus polymyxa agent; The foliar fertilizer comprises the following components in parts by weight: potassium nitrate 20 parts, potassium dihydrogen phosphate 75 parts, and trace elements 5 parts; The mixed solution is obtained by mixing 200 ml of bacillus subtilis agent, 200 ml of paenibacillus polymyxa agent, and 100 g of the phosphorus and potassium compound foliar fertilizer.

3. The control method according to claim 1 or 2, characterized by, The mass percentage concentration of the potassium permanganate solution is 2.5 ‰.

4. The control method according to claim 3, characterized by, The dosage of the potassium permanganate solution per mu is 50-60 liters.

5. The control method according to claim 3, characterized by, The dosage of the mixed solution of the microbial agent and the foliar fertilizer per mu is 500-600 ml, and the mixed solution is diluted 100 times for application.

6. The control method according to claim 3, characterized by, The potassium permanganate solution and the mixed solution of the microbial agent and the foliar fertilizer are uniformly sprayed on the tree trunks, branches, leaves, weeds, and land in the plantation.

7. The control method according to claim 3, characterized by, The step (2) further comprises spraying another period after 15-20 days from the end of the first spraying period.

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