Composition for repairing skin barrier and enhancing skin tolerance and application thereof

Abstract

The present invention relates to the field of cosmetic technology, and specifically discloses a composition that is beneficial to repairing the skin barrier and enhancing the skin's tolerance, and its application. A composition that is beneficial to repairing the skin barrier and enhancing the skin's tolerance, comprising peony root extract, rosemary extract, olive leaf extract, zelan extract and purple coneflower extract; the mass ratio of peony root extract, rosemary extract, olive leaf extract, zelan extract and purple coneflower extract is 1: (0.1~0.2): (0.1~0.2): (0.1~0.2): (0.1~0.2). The composition that is beneficial to repairing the skin barrier and enhancing the skin's tolerance prepared in the present application can significantly improve the repair effect of the skin barrier and enhance the skin's tolerance.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition and application thereof that is beneficial to repairing skin barrier and enhancing skin tolerance. Background Art

[0002] The integrity of the skin barrier function is one of the key factors in maintaining skin health, and the strength of skin tolerance is directly related to the skin's resistance to external stimuli. The weakening of the skin barrier function can lead to problems such as skin moisture loss, increased sensitivity, and inflammatory response, while the weakening of skin tolerance makes the skin more susceptible to external irritants, allergens, and microorganisms.

[0003] In modern life, due to environmental pollution, ultraviolet radiation, chemical exposure, and bad living habits, the problem of impaired skin barrier function and weakened skin tolerance is becoming increasingly serious. Therefore, developing a cosmetic that can effectively repair the skin barrier is of great significance to improving skin health.

[0004] At present, there are some products on the market for repairing the skin barrier, but these products often focus on a single ingredient or a single mechanism and have limited effects. Although they help to restore the skin barrier, they fail to truly enhance the skin's tolerance and cannot fundamentally improve the skin barrier function. When the skin is stimulated by external factors, allergic symptoms may still recur. Therefore, it is necessary to develop a cosmetic that can effectively repair the skin barrier and substantially enhance the skin's tolerance. Summary of the invention

[0005] In order to develop a cosmetic that can effectively repair the skin barrier and substantially enhance skin tolerance, the present application provides a composition that is beneficial to repairing the skin barrier and enhancing skin tolerance and its application. The present application found in the study that further adding rosemary extract, olive leaf extract, zedoaria extract and purple coneflower extract to peony root extract can significantly improve the repair effect of the skin barrier and enhance skin tolerance.

[0006] In the first aspect, the present application provides a composition that is beneficial for repairing the skin barrier and enhancing skin tolerance, which adopts the following technical solution:

[0007] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance, comprising peony root extract, rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract; the mass ratio of the peony root extract, rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract is 1: (0.1-0.2): (0.1-0.2): (0.1-0.2): (0.1-0.2).

[0008] In the above technical scheme, peony root extract has good anti-inflammatory and antioxidant properties, can help reduce skin inflammation and resist free radical damage, and adding it to the composition helps to repair damaged skin barrier. And rosemary extract, olive leaf extract, zelan extract and purple coneflower extract synergistically can significantly improve the repair effect of peony root extract on skin barrier, not only can promote skin to return to the initial state before skin barrier is damaged, but also can further promote the strengthening of skin barrier, significantly enhance skin tolerance, enhance skin resistance to external stimuli, especially resistance to external environmental pollutants. Meanwhile, the application is by peony root extract, rosemary extract, olive leaf extract, zelan extract and purple coneflower extract these five kinds of plant extracts contain nutrients, deep nourishment to skin, improve skin state, to achieve the purpose of comprehensively improving skin health.

[0009] Preferably, the mass ratio of the peony root extract, rosemary extract, olive leaf extract, zedoary extract and echinacea extract is 1:0.1:0.1:0.1:0.1.

[0010] In the above technical scheme, the present application ensures that each extract in the composition exerts the maximum effect by further optimizing the dosage ratio of each raw material, and the synergistic effect between rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract is optimal, further enhancing the repair effect of peony root extract on the skin barrier, and further enhancing the skin's tolerance, so that the composition can more effectively enhance the skin's tolerance while repairing the skin barrier, providing comprehensive protection for the skin.

[0011] Preferably, the preparation method of the peony root extract comprises the following steps:

[0012] Step 1: Take peony root, wash, dry, grind into powder, mix the peony root and water in a ratio of 1: (8-10) (w / v), sterilize, and obtain a fermentation substrate;

[0013] Step 2: adding rapeseed meal of 1% to 2% (w / w) of the mass of peony root to the fermentation substrate, inoculating a mixed fermentation strain composed of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi, and fermenting at 32 to 35° C. with stirring for 30 to 36 hours to obtain a fermentation liquid;

[0014] Step 3: Centrifuge the fermentation liquid, take the supernatant, and freeze-dry it in vacuum to obtain the peony root extract.

[0015] In the above technical scheme, the present application adopts a liquid fermentation method to extract peony root extract, selects strains composed of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi, and adds rapeseed meal and peony root extract for mixed fermentation, which effectively improves the extraction rate of active ingredients in peony root, making peony root extract easier to be absorbed by the skin. At the same time, such an extraction method also unexpectedly produces active ingredients that are more beneficial to skin barrier repair and skin tolerance enhancement, further significantly enhancing the effect of peony root extract in repairing skin barrier and enhancing skin tolerance.

[0016] Preferably, step 1 is as follows: take peony root, wash, dry, and grind into powder, mix the peony root and water in a ratio of 1: (8-10) (w / v) evenly, add 0.3%-0.5% (w / w) of the peony root mass of mixed enzyme, and perform enzymolysis for 5-6 hours at 40-50°C with stirring, sterilize, and obtain a fermentation substrate; the mixed enzyme is a mixture of cellulase, pectinase and mesophilic amylase in a mass ratio of 1: (0.7-1): (0.2-0.3).

[0017] In the above technical scheme, the present application pre-enzymes the fermentation substrate, and through the use of cellulase, pectinase and medium-temperature amylase, it can effectively decompose the different components in the peony root, and can further promote the peony root to release more active ingredients, thereby further improving the extraction efficiency of the effective ingredients in the subsequent fermentation process.

[0018] Preferably, in step 2, the usage ratio of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi is 1:(0.5-1):(0.2-0.4).

[0019] In the above technical scheme, the present application further optimizes the ratio of mixed fermentation strains to ensure the maximization of the synergistic effect during the fermentation process. The present application more effectively decomposes the complex components in the peony root through the different metabolic pathways and enzyme activities of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi, obtains active ingredients that are more conducive to skin absorption, and further enhances the repair effect of the peony root extract on the skin barrier and the enhancement effect on skin tolerance.

[0020] Preferably, the sterilization conditions of step 1 are: sterilization at 121°C for 15 min; the stirring conditions of steps 1 and 2 are: 150-200 r / min; and the centrifugation conditions of step 3 are: centrifugation at 8000-10000 r / min for 15-20 min.

[0021] In the above technical scheme, the present application obtains a peony root extract with higher activity by further optimizing the process conditions of peony root extract. Such peony root extract has better effects on skin barrier repair and skin tolerance enhancement.

[0022] In the second aspect, the application of a composition that is beneficial for repairing the skin barrier and enhancing skin tolerance provided by the present application adopts the following technical solution:

[0023] The invention discloses an application of a composition that is beneficial to repairing the skin barrier and enhancing the skin tolerance in the preparation of cosmetics, which comprises adding the composition that is beneficial to repairing the skin barrier and enhancing the skin tolerance into the cosmetics, and the cosmetics include but are not limited to lotions, emulsions, creams, essences and the like.

[0024] Preferably, the composition that is beneficial for repairing the skin barrier and enhancing skin tolerance is added in an amount of 0.14% to 10% in cosmetics.

[0025] In the above technical solution, the present application can significantly improve the skin care effect of the product by adding a composition that is beneficial to repairing the skin barrier and enhancing skin tolerance to cosmetics. Specifically, adding such a composition to cosmetics can effectively resist oxidation and inflammation, effectively relieve uncomfortable symptoms such as dry skin, sensitivity, redness and swelling, effectively repair the skin barrier and enhance skin tolerance, ensuring that the maximum skin care effect is exerted without increasing the burden on the skin.

[0026] In summary, the present application includes at least one of the following beneficial technical effects:

[0027] 1. This application further adds four raw materials, namely, rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract, to the peony root extract. Through the synergistic effect of these four raw materials, the skin barrier function is significantly improved, the skin tolerance is enhanced, and the skin's defense ability against external stimuli is enhanced, especially effectively reducing the damage of the external environment to the skin. At the same time, these five plant extracts can also deeply nourish the skin, improve skin condition, and make the skin healthier.

[0028] 2. The present application adopts a specific fermentation and enzymatic hydrolysis process to extract peony root, which not only further improves the extraction rate of active ingredients in peony root extract and enhances skin absorption, but also such a fermentation method unexpectedly produces active ingredients that are more beneficial to skin barrier repair and skin tolerance enhancement, further enhancing the skin barrier repair effect and skin tolerance enhancement effect of peony root extract. DETAILED DESCRIPTION

[0029] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] Preparation Example 1

[0031] A method for preparing a peony root extract comprises the following steps:

[0032] Step 1: Take peony root, wash and dry it, grind it into powder, pass it through a 60-mesh sieve, mix the peony root and water in a ratio of 1:8 (w / v), add 0.5% (w / w) mixed enzyme by weight of peony root, perform enzymatic hydrolysis at 40°C and 200r / min for 5h, and sterilize it at 121°C for 15min to obtain a fermentation substrate.

[0033] Step 2: Take the fermentation substrate, add rapeseed meal at 1% (w / w) of the mass of peony root, mix evenly, then add mixed fermentation bacteria solution at 1% (w / w) of the mass of peony root, and ferment at 32° C. and 200 r / min for 36 hours to obtain fermentation liquid.

[0034] Step 3: Centrifuge the fermentation liquid at 8000 r / min for 20 min, take the supernatant, add it into a freeze dryer, and vacuum freeze-dry to obtain the peony root extract.

[0035] The mixed enzyme is a mixture of cellulase, pectinase and medium-temperature amylase in a mass ratio of 1:1:0.3.

[0036] The mixed fermentation bacterial culture liquid is a mixture of Lactobacillus plantarum culture liquid, Bacillus subtilis culture liquid and Candida blancoi culture liquid in a volume ratio of 1:1:0.4.

[0037] The Lactobacillus plantarum liquid is obtained by activating and culturing Lactobacillus plantarum, and the concentration of the Lactobacillus plantarum liquid is 10 8 cfu / mL.

[0038] The Bacillus subtilis liquid is obtained by activating and culturing Bacillus subtilis, and the concentration of the Bacillus subtilis liquid is 10 8 cfu / mL.

[0039] Among them, the Candida blancoi liquid is obtained by activating and culturing Candida blancoi, and the concentration of the Candida blancoi liquid is 10 8 cfu / mL.

[0040] Among them, cellulase was purchased from Novozymes (China) Biotechnology Co., Ltd.

[0041] Among them, pectinase was purchased from Shandong Pingju Biotechnology Co., Ltd.

[0042] Among them, medium-temperature amylase was purchased from Shandong Pingju Biotechnology Co., Ltd.

[0043] Among them, Lactobacillus plantarum was purchased from the China Industrial Microbiology Culture Collection Center and is Lactobacillus plantarum CICC25160.

[0044] Among them, Bacillus subtilis was Bacillus subtilis CICC10262 purchased from the China Industrial Microbiological Culture Collection Center.

[0045] Among them, the Candida blancoi was purchased from the China Industrial Microbiological Culture Collection Center and is designated as Candida blancoi CICC33217.

[0046] Preparation Example 2

[0047] A method for preparing a peony root extract, which is different from Preparation Example 1, comprises the following steps:

[0048] Step 1: Take peony root, wash and dry it, grind it into powder, pass it through a 60-mesh sieve, mix the peony root and water in a ratio of 1:10 (w / v), add 0.3% (w / w) mixed enzyme by weight of peony root, perform enzymatic hydrolysis at 50°C and 200r / min for 6h, and sterilize it at 121°C for 15min to obtain the fermentation substrate.

[0049] Step 2: Take the fermentation substrate, add rapeseed meal with a content of 2% (w / w) of the mass of peony root, mix well, then add mixed fermentation bacteria liquid with a content of 2% (w / w) of the mass of peony root, and ferment at 35° C. and 150 r / min for 30 hours to obtain the fermentation liquid.

[0050] Step 3: Centrifuge the fermentation liquid at 10000 r / min for 15 min, take the supernatant, add it into a freeze dryer, and vacuum freeze-dry to obtain the peony root extract.

[0051] The mixed enzyme is a mixture of cellulase, pectinase and medium-temperature amylase in a mass ratio of 1:0.7:0.2.

[0052] The mixed fermentation bacterial culture liquid is a mixture of Lactobacillus plantarum culture liquid, Bacillus subtilis culture liquid and Candida blancoi culture liquid in a volume ratio of 1:0.5:0.2.

[0053] Preparation Example 3

[0054] A method for preparing a peony root extract, which is different from Preparation Example 1, comprises the following steps:

[0055] Step 1: Take peony root, wash and dry it, grind it into powder, pass it through a 60-mesh sieve, mix the peony root and water in a ratio of 1:9 (w / v), add 0.4% (w / w) mixed enzyme by weight of peony root, perform enzymatic hydrolysis at 45°C and 180r / min for 5.5h, and sterilize it at 121°C for 15min to obtain the fermentation substrate.

[0056] Step 2: Take the fermentation substrate, add rapeseed meal with a proportion of 1.5% (w / w) by weight of peony root, mix well, then add mixed fermentation bacteria solution with a proportion of 1.5% (w / w) by weight of peony root, and ferment at 34° C. and 180 r / min for 34 hours to obtain the fermentation solution.

[0057] Step 3: Centrifuge the fermentation liquid at 8000 r / min for 20 min, take the supernatant, add it into a freeze dryer, and vacuum freeze-dry to obtain the peony root extract.

[0058] The mixed enzyme is a mixture of cellulase, pectinase and medium-temperature amylase in a mass ratio of 1:0.8:0.25.

[0059] The mixed fermentation bacterial culture liquid is a mixture of Lactobacillus plantarum culture liquid, Bacillus subtilis culture liquid and Candida blancoi culture liquid in a volume ratio of 1:0.9:0.3.

[0060] Preparation Example 4

[0061] A method for preparing a peony root extract, which is different from Preparation Example 1 in that step 1 is as follows:

[0062] Peony roots were taken, washed, dried, ground into powder, passed through a 60-mesh sieve, and evenly mixed with water in a ratio of 1:8 (w / v), and sterilized at 121° C. for 15 min to obtain a fermentation substrate.

[0063] Preparation Example 5

[0064] A method for preparing a peony root extract, which is different from Preparation Example 1 in that an equal amount of rapeseed meal in step 2 is replaced by soybean meal.

[0065] Preparation Example 6

[0066] A method for preparing a peony root extract is different from Preparation Example 1 in that the mixed fermentation bacterial culture liquid is a mixture of Bacillus subtilis liquid and Candida blancoi liquid in a volume ratio of 1:0.4.

[0067] Preparation Example 7

[0068] A method for preparing a peony root extract is different from Preparation Example 1 in that the mixed fermentation bacterial culture is a mixture of Lactobacillus plantarum culture and Bacillus subtilis culture in a volume ratio of 1:0.4.

[0069] Preparation Example 8

[0070] A method for preparing a peony root extract is different from Preparation Example 1 in that the mixed fermentation bacterial culture liquid is a mixture of Lactobacillus plantarum liquid and Candida blancoi liquid in a volume ratio of 1:0.4.

[0071] Preparation Example 9

[0072] A method for preparing a peony root extract, which is different from Preparation Example 1 in that the peony root extract is prepared by water extraction. The specific preparation method comprises the following steps:

[0073] Step 1: Take peony root, wash and dry it, grind it into powder, pass it through a 60-mesh sieve, mix the peony root and water in a ratio of 1:8 (w / v), add 0.5% (w / w) mixed enzyme by weight of peony root, perform enzymolysis at 40°C and 200r / min for 5h, and sterilize it at 121°C for 15min to obtain enzymolysis solution.

[0074] Step 2: Take the enzymatic hydrolyzate and extract it in a water bath at 80°C for 3 hours to obtain an extract.

[0075] Step 3: Centrifuge the extract at 8000 r / min for 20 min, take the supernatant, add it into a freeze dryer, and vacuum freeze-dry to obtain the peony root extract.

[0076] The mixed enzyme is a mixture of cellulase, pectinase and medium-temperature amylase in a mass ratio of 1:1:0.3.

[0077] Example 1

[0078] A composition for repairing skin barrier and enhancing skin tolerance comprises 10kg of peony root extract, 1kg of rosemary extract, 1kg of olive leaf extract, 1kg of zedoaria extract and 1kg of echinacea extract.

[0079] Wherein, the peony root extract is prepared according to Preparation Example 1.

[0080] Among them, rosemary extract is prepared by water extraction with an extraction ratio of 1:10.

[0081] Among them, the olive leaf extract is prepared by water extraction with an extraction ratio of 1:10.

[0082] Among them, the zelan extract is prepared by water extraction method with an extraction ratio of 1:10.

[0083] Among them, the purple coneflower extract was prepared by water extraction with an extraction ratio of 1:10.

[0084] The preparation method of the composition that is beneficial to repairing the skin barrier and enhancing the skin tolerance is to mix all the raw materials evenly to obtain the composition that is beneficial to repairing the skin barrier and enhancing the skin tolerance.

[0085] Example 2

[0086] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance, which is different from Example 1 in that it includes 10kg of peony root extract, 2kg of rosemary extract, 2kg of olive leaf extract, 2kg of zedoaria extract and 2kg of purple coneflower extract.

[0087] Among them, the peony root extract is prepared by Preparation Example 2.

[0088] Example 3

[0089] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 3.

[0090] Example 4

[0091] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 4.

[0092] Example 5

[0093] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 5.

[0094] Example 6

[0095] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 6.

[0096] Example 7

[0097] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 7.

[0098] Example 8

[0099] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 8.

[0100] Example 9

[0101] A composition that is beneficial for repairing skin barrier and enhancing skin tolerance. The difference from Example 1 is that the peony root extract is prepared by Preparation Example 9.

[0102] Comparative Example 1

[0103] A composition, which is different from Example 9 in that the rosemary extract is replaced by the Centella asiatica extract in an equal amount.

[0104] Comparative Example 2

[0105] A composition, which is different from Example 9 in that the olive leaf extract is replaced by Centella asiatica extract in an equal amount.

[0106] Comparative Example 3

[0107] A composition, which is different from Example 9 in that an equal amount of the Zedoaria extract is replaced by a Centella asiatica extract.

[0108] Comparative Example 4

[0109] A composition, which is different from Example 9 in that an equal amount of the Echinacea purpurea extract is replaced by a Centella asiatica extract.

[0110] Experimental Samples: The compositions for repairing the skin barrier and enhancing skin tolerance of the above-mentioned embodiments and comparative examples and water were respectively mixed uniformly at a ratio of 5:95 (w / v) to obtain experimental samples.

[0111] Experimental method: A 3D skin model (Henkel, Germany, The skin damage model was constructed by treating the positive control group and the experimental group with PM2.5 (collected in a southern city) for 24 hours. Then, the experimental group was treated with 2 mL / cm 2 The experimental samples were smeared with the specification of 2mL / cm 2 The samples were smeared with pure water and cultured for 14 consecutive days. On the 15th day, PM2.5 was used to treat the positive control group and the experimental group again for 24 hours.

[0112] Before model construction, 30 minutes after successful model construction, on the 7th day, on the 14th day, and after PM2.5 re-stimulation, a transmembrane cell resistor meter (EVOM2 transmembrane cell resistor meter, WPI, USA) was used to measure the transcutaneous transmembrane resistance (TER), and a skin moisture tester (TM300 skin moisture loss tester, CK, Germany) was used to measure the transepidermal water loss (TWEL). The change rates of the measured values ​​on the 7th day, the 14th day, and after re-stimulation were calculated. The change rate calculation formula is as follows: Change rate on the 7th day and the 14th day (%) = [(subsequent measurement value - measurement value 30 minutes after successful model construction) / measurement value 30 minutes after successful model construction] * 100%, change rate after re-stimulation (%) = [(measurement value after re-stimulation - measurement value on the 14th day) / measurement value on the 14th day] * 100%. The experimental results are shown in Tables 1 and 2.

[0113] Table 1:

[0114]

[0115]

[0116]

[0117] Table 2:

[0118]

[0119]

[0120] By specifically analyzing the experimental results in Table 1 and Table 2, we can conclude that the compositions of Examples 1-9 have significant effects of repairing the skin barrier and enhancing skin tolerance.

[0121] Specifically combined with the analysis of Example 9 and Comparative Examples 1-4, Comparative Examples 1-4 respectively replaced rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract with Centella asiatica extract, and Example 9 had a good effect of repairing the skin barrier and enhancing skin tolerance; it can be seen that only the synergistic effect of rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract can significantly enhance the repair effect of peony root extract on the skin barrier, promote the recovery of the skin barrier, promote the strengthening of the skin barrier, significantly enhance skin tolerance, and enhance the skin's resistance to external stimuli, especially to external environmental pollutants.

[0122] Specifically combined with the analysis of Example 1 and Examples 4-9, Example 1 and Examples 4-9 respectively adopted different extraction processes to extract peony root extract. Example 1 has excellent effects of repairing skin barrier and enhancing skin tolerance. It can be seen that the present application further improves the extraction rate of active ingredients in peony root by optimizing the extraction process of peony root extract, and also produces active ingredients that are more beneficial to skin barrier repair and skin tolerance enhancement through unique fermentation strains, thereby further promoting skin barrier repair and skin tolerance enhancement.

[0123] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A composition that is beneficial for repairing skin barrier and enhancing skin tolerance, characterized in that: The invention comprises peony root extract, rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract; the mass ratio of the peony root extract, rosemary extract, olive leaf extract, zedoary extract and purple coneflower extract is 1: (0.1-0.2): (0.1-0.2): (0.1-0.2): (0.1-0.2), The preparation method of the peony root extract comprises the following steps: Step 1: Take peony root, wash, dry, grind into powder, mix peony root and water in a mass ratio of 1: (8-10) and sterilize to obtain fermentation substrate; Step 2: adding rapeseed meal of 1% to 2% of the weight of peony root to the fermentation substrate, inoculating a mixed fermentation strain composed of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi, and fermenting at 32 to 35° C. with stirring for 30 to 36 hours to obtain a fermentation liquid; Step 3: Centrifuge the fermentation liquid, take the supernatant, and freeze-dry it in vacuum to obtain the peony root extract.

2. A composition for repairing skin barrier and enhancing skin tolerance according to claim 1, characterized in that: The mass ratio of the peony root extract, rosemary extract, olive leaf extract, zedoary extract and echinacea extract is 1:0.1:0.1:0.1:0.

1.

3. A composition for repairing skin barrier and enhancing skin tolerance according to claim 1, characterized in that: The step 1 is as follows: Peony roots are taken, washed, dried, and ground into powder, and the peony roots and water are evenly mixed in a mass ratio of 1: (8-10), and a mixed enzyme of 0.3%-0.5% by mass of the peony roots is added, and enzymolysis is performed at 40-50° C. under stirring conditions for 5-6 hours, and sterilized to obtain a fermentation substrate; the mixed enzyme is a mixture of cellulase, pectinase and medium-temperature amylase in a mass ratio of 1: (0.7-1): (0.2-0.3).

4. A composition for repairing skin barrier and enhancing skin tolerance according to claim 1, characterized in that: In the step 2, the usage ratio of Lactobacillus plantarum, Bacillus subtilis and Candida blancoi is 1:(0.5-1):(0.2-0.4).

5. A composition for repairing skin barrier and enhancing skin tolerance according to claim 1, characterized in that: The sterilization condition of step 1 is: sterilization at 121° C. for 15 min; the stirring condition of steps 1 and 2 is: 150-200 r / min; the centrifugation condition of step 3 is: centrifugation at 8000-10000 r / min for 15-20 min.

6. Use of a composition that is beneficial for repairing skin barrier and enhancing skin tolerance in the preparation of cosmetics, characterized in that: The composition for repairing the skin barrier and enhancing skin tolerance as claimed in any one of claims 1 to 5 is added to cosmetics, and the cosmetics include but are not limited to lotions, emulsions, creams, and essences.

7. Use of the composition for repairing skin barrier and enhancing skin tolerance according to claim 6 in preparing cosmetics, characterized in that: The composition that is beneficial to repairing the skin barrier and enhancing skin tolerance is added in an amount of 0.14% to 10% in cosmetics.

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