A microbial fermentation mechanism for producing sodium hyaluronate gel

By using U-shaped floating shells and spoiler mechanisms in the microbial fermentation mechanism of sodium hyaluronate gel, the problems of bubble accumulation and uneven oxygen distribution are solved, and the fermentation effect is significantly improved.

CN119020149BActive Publication Date: 2025-03-18YANGZHOU MEIRUI BIOLOGY LTD CO
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Patent Information

Application Number
CN202411139663.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-08-20
Publication Date
2025-03-18
Estimated Expiration
2044-08-20

AI Technical Summary

Technical Problem

During the microbial fermentation process of sodium hyaluronate gel, the accumulation of bubbles and uneven oxygen distribution will affect the growth and metabolism of microorganisms and reduce the fermentation effect.

Method used

A microbial fermentation mechanism is designed, employing multiple U-shaped floating shells and spoiler mechanisms. The U-shaped floating shell pierces the bubbles through the spikes and vibrates the bubbles by slapping the liquid surface to eliminate bubble accumulation; the spoiler mechanism sprays oxygen through rotation to improve the uniform distribution of oxygen in the liquid.

Benefits of technology

Effectively remove bubbles generated during fermentation, ensure uniform transmission of gas in the fermentation broth, improve the growth and metabolic efficiency of microorganisms, and improve the fermentation effect.

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Abstract

The present invention relates to the technical field of sodium hyaluronate gel production, specifically a microbial fermentation mechanism for sodium hyaluronate gel production, comprising a tank body, a feed pipe and an exhaust pipe installed on the top of the tank body, a discharger installed at the bottom of the tank body, and an oxygen supply pipe symmetrically fixedly penetrating the inner wall of the tank body, wherein a motor is fixedly installed at the top of the tank body, and a cylindrical tube is fixedly connected to the output shaft end of the motor. A plurality of U-shaped floating shells will continuously rotate on the surface of the fermentation liquid, and adapt to the height changes of the liquid level, and the bubbles on the surface of the fermentation liquid are continuously punctured and eliminated through the spikes at the bottom of the U-shaped floating shells, and the U-shaped floating shells can also slap the liquid surface to vibrate the liquid surface, so that the bubbles generated during the fermentation process can be well removed, thereby avoiding the accumulation of bubbles on the liquid surface, avoiding affecting the transmission of gas in the fermentation liquid and reducing the ventilation effect, and avoiding affecting the growth and metabolic process of microorganisms.
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Description

Technical Field

[0001] The invention relates to the technical field of sodium hyaluronate gel production, in particular to a microbial fermentation mechanism for sodium hyaluronate gel production. Background Art

[0002] Sodium hyaluronate gel is a gel-like substance made from sodium hyaluronate. Sodium hyaluronate gel is often used in skin care products to provide deep moisturizing to the skin, repair the skin barrier, and reduce wrinkles.

[0003] The production of sodium hyaluronate gel usually uses microbial fermentation, and the microbial fermentation tank is the core equipment to achieve this process. The fermentation tank is a closed reactor that can provide a suitable environment for the growth and metabolism of microorganisms, such as temperature, pH value, oxygen supply and stirring rate. The microorganisms in the fermentation tank produce hyaluronic acid through the fermentation process, and then through subsequent extraction, purification and modification, sodium hyaluronate is obtained, and finally a gel product is made.

[0004] During the fermentation process, a culture medium containing a carbon source, a nitrogen source, minerals and other necessary nutrients needs to be prepared in the fermenter, and then the pre-cultured microorganisms (such as Streptococcus or Staphylococcus) are inoculated into the fermenter, and finally the liquid is stirred by the stirring component in the fermenter.

[0005] However, during the fermentation process, microorganisms need to be provided with sufficient oxygen, which is usually achieved by bubbling air. Since surface tension is generated when gas contacts liquid, bubbles are formed. Secondly, mechanical stirring in the fermentation tank will release dissolved gases in the liquid and form bubbles. Bubbles will gradually accumulate and float on the liquid surface. The foam will affect the transmission of gas in the fermentation liquid, reduce the ventilation effect, and further affect the growth and metabolic process of microorganisms. Secondly, the existing oxygen supply method mainly transports oxygen to the fermentation tank through an oxygen supply pipe. The oxygen is not evenly distributed in the liquid, especially when the liquid is deep. The microorganisms at the bottom often do not get enough oxygen, which affects the fermentation effect. To this end, we propose a microbial fermentation mechanism for the production of sodium hyaluronate gel. Summary of the invention

[0006] The object of the present invention is to provide a microbial fermentation mechanism for producing sodium hyaluronate gel to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a microbial fermentation mechanism for the production of sodium hyaluronate gel, comprising a tank body, a feed pipe and an exhaust pipe installed on the top of the tank body, a discharger installed at the bottom of the tank body, and an oxygen supply pipe symmetrically fixedly penetrating the inner wall of the tank body, a motor is fixedly installed at the top of the tank body, a cylindrical barrel is fixedly connected to the output shaft end of the motor, the cylindrical barrel movably penetrates the inner wall of the top of the tank body, a stirring assembly is provided at the bottom end of the cylindrical barrel, a plurality of bubble breaking mechanisms are provided between the cylindrical barrel and the tank body, and a turbulence mechanism is provided on both of the oxygen supply pipes;

[0008] The bubble breaking mechanism includes a horizontal plate, which is connected to the outer wall of the cylindrical tube through an elastic mechanism. A plurality of connecting columns are slidably inserted through the inner wall of the horizontal plate. The bottom ends of the plurality of connecting columns are fixedly connected with U-shaped floating shells. Two adjacent U-shaped floating shells fit together. The bottoms of the plurality of U-shaped floating shells are fixedly connected with a plurality of spikes. A clamping mechanism is provided between the plurality of connecting columns and the cylindrical tube.

[0009] Preferably, the stirring assembly comprises a main shaft, the main shaft is fixedly connected to the bottom end of the cylindrical barrel, and a plurality of stirring blades are fixedly sleeved on the outer wall of the main shaft.

[0010] Preferably, the elastic mechanism includes a slide groove, which is opened on the outer wall of the cylindrical tube near the lower edge, and a slider is fitted on the bottom inner wall of the slide groove, and one end of the slider located outside the cylindrical tube is fixedly connected to the end of the cross plate near the cylindrical tube, and the upper end of the slider is fixedly connected to a connecting rod, and a fixed block is slidably sleeved on the outer wall of the connecting rod near the upper edge, and one end of the fixed block is fixedly connected to the inner wall of the cylindrical tube, and a second spring is fixedly connected between the fixed block and the slider, and the second spring is slidably sleeved on the outer wall of the connecting rod.

[0011] Preferably, the clamping mechanism comprises a plurality of square rods, which are slidably inserted into a side wall of the horizontal plate, and the plurality of square rods respectively correspond to the positions of a plurality of connecting columns, and one end of the plurality of square rods corresponding to the connecting columns is fixedly connected with a rubber block, and the plurality of rubber blocks are respectively close to the plurality of connecting columns, and elastic mechanisms are provided between the plurality of square rods and the horizontal plate, and the upper ends of the plurality of square rods away from the horizontal plate are fixedly connected with triangular blocks, and the upper edges of the inclined surfaces of the plurality of triangular blocks are jointly fitted with a limiting rod, and an L-shaped rod is fixedly connected between one end of the limiting rod and the outer wall of the cylindrical tube.

[0012] Preferably, the elastic mechanism includes two U-shaped blocks, and the two U-shaped blocks are respectively fixedly connected to the two side walls of the square rod away from the cross plate, the inner walls of the two U-shaped blocks are slidably interspersed with guide columns, one end of the two guide columns are fixedly connected to the side wall of the cross plate, and the outer walls of the two guide columns are slidably sleeved with springs 1, and the two springs 1 are respectively fixedly connected between the two U-shaped blocks and the cross plate.

[0013] Preferably, the bubble breaking mechanism also includes an arc block and a plurality of limit plates, the plurality of limit plates are respectively fixedly connected to the top ends of a plurality of connecting columns, the lower ends of the plurality of limit plates are all in contact with the upper end of the cross plate, the arc block is fixedly connected to the inner wall of the tank body near the cross plate, and the upper end surface of the arc block is a slope.

[0014] Preferably, the spoiler mechanism includes a fixing frame, which is fixedly connected to the outer wall of the oxygen supply tube, a pillar is slidably inserted into the middle inner wall of the fixing frame, a connecting plate is fixedly connected to one end of the pillar close to the oxygen supply tube, a conical rubber plug is rotatably connected to the wall of the connecting plate away from the pillar, the conical surface of the conical rubber plug fits the end of the oxygen supply tube, a spring three is fixedly connected between the wall of the pillar corresponding to the connecting plate and the fixing frame, and the spring three is slidably sleeved on the outer wall of the pillar.

[0015] Preferably, the spoiler mechanism further comprises a plurality of blades, which are fixedly connected to the outer wall of the conical rubber plug in a central annular array around the conical rubber plug, and the plurality of blades are all arranged at an inclination.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The microbial fermentation tank produced by the sodium hyaluronate gel is provided with a bubble breaking mechanism, an elastic mechanism, a clamping mechanism and an elastic mechanism that cooperate with each other. A plurality of U-shaped floating shells will rotate continuously on the surface of the fermentation liquid and adapt to the height changes of the liquid surface. The bubbles on the surface of the fermentation liquid are continuously punctured and eliminated by the spikes at the bottom of the U-shaped floating shells. At the same time, the U-shaped floating shells can also slap the liquid surface to vibrate the liquid surface, thereby effectively removing the bubbles generated during the fermentation process, thereby avoiding the accumulation of bubbles on the liquid surface, avoiding affecting the transmission of gas in the fermentation liquid and reducing the ventilation effect, and avoiding affecting the growth and metabolic process of the microorganisms.

[0018] 2. By setting up a turbulent mechanism, oxygen will be sprayed out in a ring-shaped and rotating manner, so that the oxygen will be distributed more evenly in the liquid, and the fermentation liquid at the bottom of the tank will be fully exposed to oxygen, thereby improving the fermentation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 It is a cross-sectional view of the tank body, the exhaust pipe and the feed pipe of the present invention;

[0021] Figure 3 It is a structural display diagram of the cylindrical barrel of the present invention;

[0022] Figure 4 For the present invention Figure 3 A magnified view of the structure at A;

[0023] Figure 5 A cross-sectional view of a cylindrical barrel and one of the horizontal plates of the present invention;

[0024] Figure 6 For the present invention Figure 5 A magnified view of the structure at B in FIG.

[0025] Figure 7 is a cross-sectional view of the oxygen supply tube and the fixing frame of the present invention;

[0026] Figure 8 It is a cross-sectional view of the tank body of the present invention.

[0027] In the attached drawings, the list of parts represented by each reference numeral is as follows: 1. Motor; 2. Exhaust pipe; 3. Discharger; 4. Oxygen supply pipe; 5. Tank; 6. Feed pipe; 7. Cylindrical tube; 8. Arc block; 9. Cross plate; 10. Connecting column; 11. U-shaped floating shell; 12. Main shaft; 13. Stirring blade; 14. Limiting plate; 15. Slide; 16. L-shaped rod; 17. Limiting rod; 18. Spike; 19. Triangular block; 20. U-shaped block; 21. Square rod; 22. Guide column; 23. Spring one; 24. Rubber block; 25. Slider; 26. Spring two; 27. Fixed block; 28. Connecting rod; 29. ​​Fixed frame; 30. Connecting plate; 31. Conical rubber plug; 32. Blade; 33. Spring three; 34. Pillar. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1: Please refer to Figure 1 - Figure 8 A microbial fermentation mechanism for producing sodium hyaluronate gel, comprising a tank body 5, a feed pipe 6 and an exhaust pipe 2 installed on the top of the tank body 5, a discharger 3 installed at the bottom of the tank body 5, and an oxygen supply pipe 4 symmetrically fixedly penetrating the inner wall of the tank body 5, a motor 1 is fixedly installed at the top of the tank body 5, a cylindrical barrel 7 is fixedly connected to the output shaft end of the motor 1, the cylindrical barrel 7 movably penetrates the inner wall of the top of the tank body 5, a stirring assembly is arranged at the bottom end of the cylindrical barrel 7, a plurality of bubble breaking mechanisms are arranged between the cylindrical barrel 7 and the tank body 5, and a turbulent mechanism is arranged on both oxygen supply pipes 4;

[0030] The bubble breaking mechanism includes a transverse plate 9, which is connected to the outer wall of the cylindrical tube 7 through an elastic mechanism. A plurality of connecting columns 10 are slidably inserted through the inner wall of the transverse plate 9. The bottom ends of the plurality of connecting columns 10 are fixedly connected with U-shaped floating shells 11. Two adjacent U-shaped floating shells 11 fit each other. The bottoms of the plurality of U-shaped floating shells 11 are fixedly connected with a plurality of spikes 18. A clamping mechanism is provided between the plurality of connecting columns 10 and the cylindrical tube 7.

[0031] The stirring assembly comprises a main shaft 12 , which is fixedly connected to the bottom end of the cylindrical barrel 7 , and a plurality of stirring blades 13 are fixedly sleeved on the outer wall of the main shaft 12 .

[0032] Specifically, when the cylindrical barrel 7 rotates, the main shaft 12 and the plurality of stirring blades 13 are driven to rotate together, thereby facilitating stirring of the fermentation liquid.

[0033] The elastic mechanism includes a slide groove 15, which is opened on the outer wall of the cylindrical tube 7 near the lower edge. A slider 25 is fitted on the bottom inner wall of the slide groove 15. The end of the slider 25 located outside the cylindrical tube 7 is fixedly connected to the end of the cross plate 9 near the cylindrical tube 7. The upper end of the slider 25 is fixedly connected to a connecting rod 28. A fixed block 27 is slidably sleeved on the outer wall of the connecting rod 28 near the upper edge. One end of the fixed block 27 is fixedly connected to the inner wall of the cylindrical tube 7. A spring 26 is fixedly connected between the fixed block 27 and the slider 25. The spring 26 is slidably sleeved on the outer wall of the connecting rod 28.

[0034] The clamping mechanism includes multiple square rods 21, which are slidably inserted into one side wall of the cross plate 9. The multiple square rods 21 correspond to the positions of the multiple connecting columns 10 respectively. One ends of the multiple square rods 21 corresponding to the connecting columns 10 are fixedly connected with rubber blocks 24. The multiple rubber blocks 24 are respectively close to the multiple connecting columns 10. Elastic mechanisms are provided between the multiple square rods 21 and the cross plate 9. The upper ends of the multiple square rods 21 are fixedly connected with triangular blocks 19 away from the cross plate 9. The upper edges of the inclined surfaces of the multiple triangular blocks 19 are jointly fitted with a limiting rod 17, and an L-shaped rod 16 is fixedly connected between one end of the limiting rod 17 and the outer wall of the cylindrical tube 7.

[0035] The elastic mechanism includes two U-shaped blocks 20, and the two U-shaped blocks 20 are respectively fixedly connected to the two side walls of the square rod 21 away from the horizontal plate 9. The inner walls of the two U-shaped blocks 20 are slidably interspersed with guide columns 22, and one end of the two guide columns 22 is fixedly connected to the side wall of the horizontal plate 9. The outer walls of the two guide columns 22 are slidably sleeved with springs 23, and the two springs 23 are respectively fixedly connected between the two U-shaped blocks 20 and the horizontal plate 9.

[0036] The bubble breaking mechanism also includes an arc block 8 and a plurality of limit plates 14. The plurality of limit plates 14 are respectively fixedly connected to the top ends of the plurality of connecting columns 10. The lower ends of the plurality of limit plates 14 are all in contact with the upper ends of the transverse plates 9. The arc block 8 is fixedly connected to the inner wall of the tank body 5 near the transverse plate 9. The upper end surface of the arc block 8 is a slope.

[0037] In this embodiment, raw materials such as culture medium and pre-cultured microorganisms can be placed into the tank body 5 along the feed pipe 6, and then the feed pipe 6 is sealed with a tube cover. The oxygen supply pipe 4 can be connected to the pipeline of the existing oxygen delivery equipment to transport oxygen into the tank body 5. The exhaust pipe 2 can be connected to the pipeline of the existing equipment for treating exhaust gas. The tank body 5 has its own temperature control system. This is a well-known existing technology and will not be elaborated on.

[0038] Then, the motor 1 is started to drive the cylindrical barrel 7 and the stirring assembly to rotate together, so as to stir the fermentation liquid and promote mixing. When the cylindrical barrel 7 rotates, it can drive the multiple fixed blocks 27 to rotate. Each fixed block 27 can drive the connected connecting rod 28, spring 26, slider 25 and cross plate 9 to rotate together. Each cross plate 9 can drive the connected multiple connecting columns 10 and multiple U-shaped floating shells 11 to rotate together. The multiple U-shaped floating shells 11 can float on the liquid surface. It is worth mentioning that when the culture medium and the pre-cultured microorganisms are placed in the tank body 5, as the liquid level rises, the liquid surface will contact the bottom of the multiple U-shaped floating shells 11, and as the liquid level continues to rise, the multiple U-shaped floating shells 11 will float up, and each U-shaped floating shell 11 will drive the connected connecting column 10 to slide up in the horizontal plate 9. When the middle of the connecting column 10 rises to correspond to the horizontal plate 9, the liquid level stops rising, and the culture medium and the pre-cultured microorganisms are added at this time. When the stirring component stirs the liquid, the liquid level height near the stirring component area will be lower than the liquid level height away from the stirring component area (this is because the centrifugal force brought by the stirring liquid causes the liquid to flow outward from the stirring center area, causing the liquid level near the stirring component to lower). At this time, the height of the U-shaped floating shell 11 near the stirring component will be lower than the height of the U-shaped floating shell 11 away from the stirring component, thereby adapting to the change in liquid level. At the same time, when the U-shaped buoy 11 rotates on the liquid surface, the spikes 18 at the bottom thereof will continuously puncture the bubbles generated during the fermentation process, thereby preventing the bubbles from accumulating on the liquid surface, affecting the transmission of gas in the fermentation liquid and reducing the ventilation effect, and affecting the growth and metabolic process of the microorganisms.

[0039] It should be noted that each cross plate 9 will pass through the slope of multiple arc blocks 8 in sequence when rotating. When each cross plate 9 passes through an arc block 8, the cross plate 9 will first move up along the slope. At this time, the cross plate 9 will drive the slider 25 to slide upward in the corresponding slide groove 15, and the slider 25 will drive the corresponding connecting rod 28 to slide up in the corresponding fixed block 27 and squeeze the spring 26. The cross plate 9 will also drive multiple square rods 21 and multiple guide columns 22 to move upward. The multiple square rods 21 can drive the rubber blocks 24, triangular blocks 19 and U-shaped blocks 20 connected to each other to move upward together. The inclined surfaces of the multiple triangular blocks 19 will squeeze the limiting rod 17, and the limiting rod 17 is fixed to the cylindrical tube 7 through the L-shaped rod 16. Then, the multiple triangular blocks 19 will move toward the direction of the cross plate 9 and always keep in contact with the limiting rod 17. The multiple triangular blocks 19 can drive the U-shaped blocks 20 connected to each other to slide outside the corresponding guide column 22 and squeeze the spring 1 23, and the multiple triangular blocks 1 9 can drive the square rods 21 connected to each other to slide into the cross plate 9, and the multiple square rods 21 can drive the rubber blocks 24 connected to each other to squeeze the corresponding connecting columns 10, thereby clamping the multiple connecting columns 10. As the cross plate 9 continues to move upward, the clamped multiple connecting columns 10 will also move upward, and the multiple U-shaped floating shells 11 will also move upward. As the cross plate 9 continues to move upward, the multiple rubber blocks 24 will squeeze and deform and always clamp the corresponding connecting columns 10 until the cross plate 9 leaves the arc block 8. At this time, under the action of the spring second 26, the cross plate 9 will automatically move downward. Under the action of the spring one 23, each cross plate 9 will move away from the corresponding connecting column 10, then each rubber block 24 will loosen the corresponding connecting column 10, then the multiple connecting columns 10 and the multiple U-shaped floating shells 11 will move downward, and the multiple U-shaped floating shells 11 will hit the liquid surface, thereby beating and vibrating the liquid surface, thereby further eliminating bubbles, and further preventing bubbles from affecting the growth and metabolic process of microorganisms. At the same time, when the fermentation liquid is subsequently discharged along the discharge device 3, the up and down movement of the multiple U-shaped floating shells 11 will also cause the liquid adhering to the bottom to be quickly shaken off, reducing adhesion and making the discharge more thorough.

[0040] Example 2: Please refer to Figure 7 The spoiler mechanism includes a fixing frame 29, which is fixedly connected to the outer wall of the oxygen supply pipe 4. A pillar 34 is slidably inserted into the middle inner wall of the fixing frame 29. A connecting plate 30 is fixedly connected to one end of the pillar 34 close to the oxygen supply pipe 4. The connecting plate 30 is rotatably connected to a conical rubber plug 31 away from the wall of the pillar 34. The conical surface of the conical rubber plug 31 fits the end of the oxygen supply pipe 4. A spring three 33 is fixedly connected between the wall of the connecting plate 30 corresponding to the pillar 34 and the fixing frame 29. The spring three 33 is slidably sleeved on the outer wall of the pillar 34.

[0041] The spoiler mechanism further includes a plurality of blades 32 , which are fixedly connected to the outer wall of the conical rubber plug 31 in a central annular array around the conical rubber plug 31 , and the plurality of blades 32 are all arranged at an inclination.

[0042] In this embodiment, when oxygen is introduced into the oxygen supply tube 4, under the action of air pressure, the conical rubber plug 31 will be pushed outward, the conical rubber plug 31 will drive the connecting plate 30 to move together, the connecting plate 30 will drive the support 34 to slide outward in the fixing frame 29, and the connecting plate 30 will also squeeze the spring three 33 connected to the fixing frame 29, so that oxygen is ejected from the gap between the outer wall of the conical rubber plug 31 and the end of the oxygen supply tube 4, and the oxygen will be ejected in a ring form along the conical surface of the conical rubber plug 31. At the same time, the oxygen will also pass through the gaps between the multiple blades 32, causing the multiple blades 32 to rotate with the conical rubber plug 31, so that the oxygen will be in a ring shape and ejected in a rotating manner, so that the oxygen will be more evenly distributed in the liquid, and the fermentation liquid at the bottom of the tank body 5 will also be fully exposed to the oxygen, thereby improving the fermentation effect.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microbial fermentation mechanism for producing sodium hyaluronate gel, comprising a tank body (5), a feed pipe (6) and an exhaust pipe (2) installed on the top of the tank body (5), a discharger (3) installed on the bottom of the tank body (5), and an oxygen supply pipe (4) symmetrically fixed and penetrating the inner wall of the tank body (5), characterized in that: A motor (1) is fixedly mounted on the top of the tank body (5); a cylindrical barrel (7) is fixedly connected to the output shaft end of the motor (1); the cylindrical barrel (7) movably penetrates the inner wall of the top of the tank body (5); a stirring assembly is provided at the bottom of the cylindrical barrel (7); a plurality of bubble breaking mechanisms are provided between the cylindrical barrel (7) and the tank body (5); and a flow disturbance mechanism is provided on both of the two oxygen supply pipes (4); The bubble breaking mechanism comprises a horizontal plate (9), wherein the horizontal plate (9) is connected to the outer wall of the cylindrical tube (7) through an elastic mechanism, and a plurality of connecting columns (10) are slidably inserted through the inner wall of the horizontal plate (9), and the bottom ends of the plurality of connecting columns (10) are fixedly connected to a U-shaped floating shell (11), and two adjacent U-shaped floating shells (11) are fitted together, and the bottoms of the plurality of U-shaped floating shells (11) are fixedly connected to a plurality of spikes (18), and a clamping mechanism is provided between the plurality of connecting columns (10) and the cylindrical tube (7); The elastic mechanism comprises a slide groove (15), wherein the slide groove (15) is provided on the outer wall of the cylindrical tube (7) near the lower edge, and a slider (25) is fitted on the inner wall of the bottom of the slide groove (15), and one end of the slider (25) located outside the cylindrical tube (7) is fixedly connected to one end of the cross plate (9) near the cylindrical tube (7), and the upper end of the slider (25) is fixedly connected to a connecting rod (28), and a fixing block (27) is slidably sleeved on the outer wall of the connecting rod (28) near the upper edge, and one end of the fixing block (27) is fixedly connected to the inner wall of the cylindrical tube (7), and a second spring (26) is fixedly connected between the fixing block (27) and the slider (25), and the second spring (26) is slidably sleeved on the outer wall of the connecting rod (28); The clamping mechanism comprises a plurality of square rods (21), the plurality of square rods (21) are slidably inserted into a side wall of the horizontal plate (9), the plurality of square rods (21) respectively correspond to the positions of the plurality of connecting columns (10), one end of the plurality of square rods (21) corresponding to the connecting columns (10) is fixedly connected to a rubber block (24), the plurality of rubber blocks (24) are respectively close to the plurality of connecting columns (10), elastic mechanisms are arranged between the plurality of square rods (21) and the horizontal plate (9), the upper ends of the plurality of square rods (21) away from the horizontal plate (9) are fixedly connected to a triangular block (19), the upper edges of the inclined surfaces of the plurality of triangular blocks (19) are jointly fitted with a limiting rod (17), and an L-shaped rod (16) is fixedly connected between one end of the limiting rod (17) and the outer wall of the cylindrical tube (7); The elastic mechanism comprises two U-shaped blocks (20), the two U-shaped blocks (20) are respectively fixedly connected to the two side walls of the square rod (21) away from the horizontal plate (9), the inner walls of the two U-shaped blocks (20) are slidably interspersed with guide columns (22), one end of the two guide columns (22) are fixedly connected to the side wall of the horizontal plate (9), and the outer walls of the two guide columns (22) are slidably sleeved with springs (23), and the two springs (23) are respectively fixedly connected between the two U-shaped blocks (20) and the horizontal plate (9); The bubble breaking mechanism further comprises an arc block (8) and a plurality of limit plates (14); the plurality of limit plates (14) are respectively fixedly connected to the top ends of the plurality of connecting columns (10); the lower ends of the plurality of limit plates (14) are all in contact with the upper ends of the transverse plates (9); the arc block (8) is fixedly connected to the inner wall of the tank body (5) near the transverse plate (9); and the upper end surface of the arc block (8) is a sloped surface.

2. The microbial fermentation mechanism for producing sodium hyaluronate gel according to claim 1, characterized in that: The stirring assembly comprises a main shaft (12), the main shaft (12) being fixedly connected to the bottom end of the cylindrical barrel (7), and a plurality of stirring blades (13) being fixedly sleeved on the outer wall of the main shaft (12).

3. The microbial fermentation mechanism for producing sodium hyaluronate gel according to claim 1, characterized in that: The spoiler mechanism comprises a fixing frame (29), the fixing frame (29) being fixedly connected to the outer wall of the oxygen supply pipe (4), a pillar (34) being slidably inserted through the middle inner wall of the fixing frame (29), a connecting plate (30) being fixedly connected to one end of the pillar (34) close to the oxygen supply pipe (4), a conical rubber plug (31) being rotatably connected to the connecting plate (30) away from the wall surface of the pillar (34), the conical surface of the conical rubber plug (31) being fitted to the end of the oxygen supply pipe (4), a spring three (33) being fixedly connected between the wall surface of the connecting plate (30) corresponding to the pillar (34) and the fixing frame (29), and the spring three (33) being slidably sleeved on the outer wall of the pillar (34).

4. The microbial fermentation mechanism for producing sodium hyaluronate gel according to claim 3, characterized in that: The spoiler mechanism further comprises a plurality of blades (32), wherein the plurality of blades (32) are fixedly connected to the outer wall of the conical rubber plug (31) in a central annular array around the conical rubber plug (31), and the plurality of blades (32) are all arranged at an inclination.

Citation Information

Patent Citations

  • Constant pressure fermentation tank

    CN108004128A

  • Stirring type microbial fermentation tank capable of efficiently dissolving oxygen

    CN118006444A

  • Compound air inlet unit of airlift fermentation jar

    CN206244797U